Brassinosteroid negatively regulates jasmonate inhibition of root growth in Arabidopsis
作者：
Huang, Ying;Han, Chengyun;Peng, Wen;Peng, Zhihong;Xiong, Xingyao;...
期刊：
PLANT SIGNALING & BEHAVIOR ,2010年5(2):140-142 ISSN：1559-2316
通讯作者：
Ren, Chunmei
作者机构：
[Ren, Chunmei; Han, Chengyun; Peng, Zhihong; Huang, Ying] Hunan Agr Univ, Crop Gene Engn Key Lab Hunan Prov, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China.;[Han, Chengyun; Peng, Wen; Huang, Ying; Xie, Daoxin] Tsinghua Univ, Sch Life Sci, Beijing, Peoples R China.;[Xiong, Xingyao; Zhu, Qi] Hunan Agr Univ, Coll Hort & Landscape, Changsha, Hunan, Peoples R China.;[Gao, Bida] Hunan Agr Univ, Coll Biosafety Sci & Technol, Changsha, Hunan, Peoples R China.
通讯机构：
[Ren, Chunmei] H;Hunan Agr Univ, Crop Gene Engn Key Lab Hunan Prov, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China.
关键词：
jasmonate signaling;root growth;brassinosteroid;brassinazole;arabidopsis
摘要：
Jasmonate (JA) inhibits root growth of Arabidopsis thaliana seedlings. The mutation in COI1, that plays a central role in JA signaling, displays insensitivity to JA inhibition of root growth. To dissect JA signaling pathway, we recently isolated one mutant named psc1, which partially suppresses coi1 insensitivity to JA inhibition of root growth. As we identified the PSC1 gene as an allele of DWF4 that encodes a key enzyme in brassinosteroid (BR) biosynthesis, we hypothesized and demonstrated that BR is involved in JA signaling and negatively regulates JA inhibition of root growth. In our Plant Physiology paper, we analyzed effects of psc1 or exogenous BR on the inhibition of root growth by JA. Here we show that treatment with brassinazole (Brz), a BR biosynthesis inhibitor, increased JA sensitivity in both coi1-2 and wild type, which further confirms that BR negatively regulates JA inhibition of root growth. Since effects of psc1, Brz and exogenous BR on JA inhibition of root growth were mild, we suggests that BR negatively finely regulates JA inhibition of root growth in Arabidopsis. © 2010 Landes Bioscience.
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英文
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Profiling and characterisation by liquid chromatography/multi-stage mass spectrometry of the chlorogenic acids in Gardeniae Fructus
作者：
Clifford, Michael N.* ;Wu, Weiguo;Kirkpatrick, Jo;Jaiswal, Rakesh;Kuhnert, Nikolai
期刊：
Rapid Communications in Mass Spectrometry ,2010年24(21):3109-3120 ISSN：0951-4198
通讯作者：
Clifford, Michael N.
作者机构：
[Clifford, Michael N.; Kirkpatrick, Jo] Univ Surrey, Ctr Nutr & Food Safety, Guildford GU2 5XH, Surrey, England.;[Jaiswal, Rakesh; Kuhnert, Nikolai] Jacobs Univ Bremen, Sch Engn & Sci Chem, D-28759 Bremen, Germany.;[Wu, Weiguo] Hunnan Agr Univ, Coll Food Sci & Technol, Changsha 41028, Hunan, Peoples R China.
通讯机构：
[Clifford, Michael N.] U;Univ Surrey, Ctr Nutr & Food Safety, Guildford GU2 5XH, Surrey, England.
摘要：
The chlorogenic acids of Gardeniae Fructus used traditionally as a Chinese herbal medicine (zhizi) have been investigated qualitatively by liquid chromatography/multi-stage mass spectrometry (LC/MS4). Twenty-nine chlorogenic acids were detected and twenty-five characterised to regioisomer level on the basis of their fragmentation, twenty-four for the first time from this source. Assignment to the level of individual regioisomers was possible for three caffeoylquinic acids, three dicaffeoylquinic acids, three sinapoylquinic acids, four caffeoyl-sinapoylquinic acids, two feruloyl-sinapoylquinic acids, one p-coumaroyl-sinapoylquinic acid, three (3-hydroxy, 3-methyl)glutaroyl-quinic acids, two (3-hydroxy, 3-methyl)glutaroyl-feruloylquinic acids, one (3-hydroxy, 3-methyl)glutaroyl-dicaffeoylquinic acid, and one (3-hydroxy, 3-methynglutaroyl-caffeoyl-feruloylquinic acid. Six (3-hydroxy, 3-methyl)glutaroyl-caffeoylquinic acids were detected and two were tentatively assigned as 3-caffeoyl-4-(3-hydroxy, 3-methyl)glutaroylquinic acid and 3-caffeoyl-5-(3-hydroxy, 3-methyl)glutaroylquinic acid. The (3-hydroxy, 3-methyl)glutaroyl residue modifies the mass spectral fragmentation behavior and elution sequence compared with the chlorogenic acids that contain only a cinnamic acid residue(s). Fourteen of these twenty-nine chlorogenic acids have not previously been reported from any source. Copyright (C) 2010 John Wiley & Sons, Ltd.
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Molecular and biological characterization of a severe isolate of Tomato chlorotic dwarf viroid containing a novel terminal right (TR) domain sequence
作者：
Singh, Rudra P.* ;Dilworth, Andrea D.;Ao, Xiaoping;Singh, Mathuresh;Misra, Santosh
期刊：
European Journal of Plant Pathology ,2010年127(1):63-72 ISSN：0929-1873
通讯作者：
Singh, Rudra P.
作者机构：
[Singh, Rudra P.; Dilworth, Andrea D.] Agr & Agri Food Canada, Potato Res Ctr, Fredericton, NB E3B 4Z7, Canada.;[Ao, Xiaoping] Hunan Agr Univ, Fac Hort & Landscape, Changsha 410128, Hunan, Peoples R China.;[Singh, Mathuresh] Agr Certificat Serv, Fredericton, NB E3B 8B7, Canada.;[Misra, Santosh] Univ Victoria, Dept Biochem & Microbiol, Victoria, BC V8W 3P6, Canada.;[Singh, Rudra P.] Agr & Agri Food Canada, Potato Res Ctr, 850 Lincoln Rd, Fredericton, NB E3B 4Z7, Canada.
通讯机构：
[Singh, Rudra P.] A;Agr & Agri Food Canada, Potato Res Ctr, 850 Lincoln Rd, Fredericton, NB E3B 4Z7, Canada.
关键词：
Evolution of viroid;Extreme stunted plants;Sequence variants;Viroid strain;Variation of symptoms
摘要：
Tomato chlorotic dwarf viroid (TCDVd) manually inoculated to transgenic (cv.'Desiree') potato plants containing antimicrobial cationic peptides failed to develop symptoms in above ground plant parts, but infected tubers were symptomatic. Plants from the infected tubers (second generation plants) emerged as either severely stunted (bushy stunt isolate, BSI) or tall and symptomless. Molecular characterization of BSI isolates showed TCDVd sequence variants 95 to 98% identical to TCDVd sequences from the database, while a viroid variant identical to TCDVd type isolate (acc # AF162131) was cloned from symptomless plants. The TCDVd BSI variants had novel U165C, GU177-178AA, and UCAC181-184CUUU nucleotide substitutions in the terminal right (TR) domain of the viroid molecule. The cloned viroid cDNAs of the BSI were infectious to experimental (cv. 'Sheyenne') tomato plants causing stunted plants with profuse auxiliary shoots. Visual evaluation of the susceptibility of the BSI to 18 potato and 21 tomato cultivars revealed severe symptoms in most cultivars of both species. The progeny variants accumulating in each potato and tomato cultivar exhibited the same novel TR domain in most cultivars, with only a slight variation in a few. The severity of the stunting symptoms induced by TCDVd from BSI isolates in both potato and tomato cultivars has not been noted previously with other TCDVd isolates and, as such, it is proposed that this new isolate be recognized as a distinct genotype. Emergence of this type of sequence variant in commercial fields or commercial tomato greenhouses could potentially cause relevant losses in both crops. © 2009 KNPV.
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英文
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Simultaneous elution of polycyclic aromatic hydrocarbons and heavy metals from contaminated soil by two amino acids derived fromβ-cyclodextrins
作者：
Yang, Chengjian;Zeng, Qingru* ;Wang, Yunzhong;Liao, Bohan;Sun, Jian;...
期刊：
环境科学学报(英文版) ,2010年22(12):1910-1915 ISSN：1001-0742
通讯作者：
Zeng, Qingru
作者机构：
[Liao, Bohan; Zeng, Qingru; Yang, Chengjian] Hunan Agr Univ, Dept Environm Sci, Changsha 410128, Hunan, Peoples R China.;[Wang, Yunzhong; Shi, Hui; Chen, Xingdou; Yang, Chengjian] Xian Architecture Sci Technol Univ, Sch Environm & Municipal Engn, Xian 710055, Peoples R China.;[Sun, Jian] S China Univ Technol, Minist Educ, Key Lab Pollut Control & Ecol Remediat Ind Agglom, Dept Environm Sci & Engn, Guangzhou 510006, Guangdong, Peoples R China.
通讯机构：
[Zeng, Qingru] H;Hunan Agr Univ, Dept Environm Sci, Changsha 410128, Hunan, Peoples R China.
关键词：
anthracene;cadmium;cyclodextrin;solubilization;elution;soil
摘要：
Two highly water-soluble amino acids,which derived fromβ-CDs,i.e.,glutamic acid-β-cyclodextrin(GluCD)and ethylene-diamine-β-cyclodextrin(EDCD),were synthesized and were examined for their effect on solubilization of anthracene(ANT),complexation of cadmium(Cd~(2+)),and elution removal of ANT and Cd~(2+)in soil.The results showed that GluCD and EDCD were powerful complexant for ANT and Cd~(2+).In the presence of 10 g/L GluCD and EDCD,the solubilization of ANT increased by 47.04 and 23.85 times compared to the control,respectively.GluCD resulted in approximately 90%complexation of Cd~(2+)while 70%complexation was observed for EDCD.Simultaneously,GluCD and EDCD could greatly enhance the elution removal of ANT and Cd~(2+)from soil.GluCD resulted in the highest elution effciency of ANT and Cd~(2+).With the addition of 10 g/L GluCD,53.5%of ANT and 85.6%of Cd~(2+)were eluted,respectively.The ANT had a negligible effect on the Cd~(2+)removal due to different complexing sites of ANT and Cd~(2+),while Cd~(2+)enhanced the ANT removal under the addition of GluCD because Cd~(2+)neutralized the-COOH group of GluCD.Adversely,the removal of ANT was decreased with Cd~(2+)under the addition of EDCD,this was due to the fact that Cd~(2+)enhanced the polarity of EDCD molecule and inhibited the complexation between ANT and EDCD.The study suggested that GluCD could be preferred and be successfully applied to remediation of heavy metals or organic compounds in contaminated soil
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英文
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Gynostemma pentaphyllum protects mouse male germ cells against apoptosis caused by zearalenone via Bax and Bcl-2 regulation
作者：
Yuan, Hui;Deng, Youtian;Yuan, Liyun;Wu, Jing;Yuan, Zhihang;...
期刊：
Toxicology Mechanisms and Methods ,2010年20(3):153-158 ISSN：1537-6516
通讯作者：
He, Zuping
作者机构：
[Wu, Jing; Zhu, Li; Yuan, Liyun; Li, Rongfang; Yi, Jine; Wen, Lixin; Deng, Youtian; Guo, Chengzhi; Yuan, Hui; Yuan, Zhihang; Zhang, Ming] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[He, Zuping] Georgetown Univ, Med Ctr, Dept Biochem & Mol & Cellular Biol, Washington, DC 20057 USA.;[He, Zuping] Georgetown Univ, Med Ctr, Dept Biochem & Mol & Cellular Biol, 3900 Reservoir Rd,NW, Washington, DC 20057 USA.
通讯机构：
[He, Zuping] G;Georgetown Univ, Med Ctr, Dept Biochem & Mol & Cellular Biol, 3900 Reservoir Rd,NW, Washington, DC 20057 USA.
关键词：
Gynostemma pentaphyllum;anti-toxicity;Zearalenone;male germ cells;Bax and Bcl-2
摘要：
The objective of this study was to explore the effects of Gynostemma pentaphyllum on Zearalenone-induced apoptosis in mouse male germ cells. Fifty Kunming male mice at 25-days-old were classified into five groups: group A was the control (10% ethanol, 0.5 ml/day); group B with 10 μg Zearalenone/day; group C with 10 μg Zearalenone and 50 mg/kg/day Gynostemma pentaphyllum; group D with 10 μg Zearalenone and 100 mg/kg/day Gynostemma pentaphyllum; and group E with 10 μg Zearalenone and 200 mg/kg/day Gynostemma pentaphyllum. It was found that Gynostemma pentaphyllum has a marked effect on protecting male germ cells against Zearalenone-induced apoptosis, as evidenced by a reduced apoptosis rate of male germ cells and Bax expression as well as an enhancement of Bcl-2 expression in Gynostemma pentaphyllum-treated groups compared to the control. In addition, Gynostemma pentaphyllum remarkably improved pathologic changes of testicular tissue, reduced the content of malondialdehyde (MDA), and increased the activity of superoxide dismutase (SOD) caused by Zearalenone. Taken together, these results suggest that Gynostemma pentaphyllum protects against toxicity caused by Zearalenone through anti-oxidation and anti-apoptosis via the regulation of Bax and Bcl-2 expression. © 2010 Informa UK Ltd.
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英文
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Effect of copper, ferrous iron and zinc from different sources on growth performance and fecal copper, ferrous iron and zinc balance in growing pigs
作者：
Deng, Yuelin;Mi, Many;Chen, Feng;Deng, Dun;Ying, Peng* ;...
期刊：
Journal of Food, Agriculture and Environment ,2010年8(3-4):71-77 ISSN：1459-0255
通讯作者：
Ying, Peng
作者机构：
[Chen, Feng; Deng, Yuelin; Deng, Dun] Guangdong Wens Foodstuffs Grp Co Ltd, Guangzhou 527439, Guangdong, Peoples R China.;[Ying, Peng] Hunan Open Univ, Kaifung 410004, Hunan, Peoples R China.;[Chen, Feng; Deng, Yuelin] S China Agr Univ, Guangzhou 510640, Peoples R China.;[Fang, Jun] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410126, Hunan, Peoples R China.;[Wang, Mi; Mi, Many] Chinese Acad Agr Sci, Shanghai Vet Res Inst, Shangshai 200241, Peoples R China.
通讯机构：
[Ying, Peng] H;Hunan Open Univ, Kaifung 410004, Hunan, Peoples R China.
关键词：
Copper;Ferrous iron;Growing pig;Proteinate;Zinc
摘要：
The objective of the present study was to evaluate the effect of dietary supplemented with copper, ferrous iron and zinc together from their proteinates or sulphates on growth performance, plasma concentration and balance of copper, ferrous iron and zinc in pigs at different growth stages. Three growth experiments and one mineral balance test were included in present study. In comparison with diet supplemented with copper, ferrous iron and zinc from sulfates together, diet supplemented with lower concentrations of copper, ferrous iron and zinc together from their proteinates significantly increased (P < 0.05) the feed efficiency in all three growth experiments and also increased (P < 0.05) the average daily gain (ADG) in Exp. 1, however, no difference of average daily feed intake (ADFI) in Exp. 1 and ADG ADFI and plasma concentrations of copper, ferrous iron and zinc were observed in Exp. 2 and 3, respectively. In mineral balance trail, pigs consumed copper, ferrous iron and zinc from their proteinates decreased copper, ferrous iron and zinc excretion by 91 % (P < 0.01 ), 45% (P < 0.01 ) and 71 % (P < 0.01 ) compared with pigs that consumed these minerals from sulfates, respectively. We concluded that dietary supplemented with copper, ferrous iron and zinc from their proteinate sources at the levels of 20, 100 and 40 mg/kg together have a growth-promoting effect on pigs at early growth stage and decrease copper, ferrous and zinc elimination into the environment.
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英文
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Entanglement dynamics of a double two-photon Jaynes-Cummings model with Kerr-like medium
作者：
Ouyang Xi-Cheng;Fang Mao-Fa* ;Kang Guo-Dong;Deng Xiao-Juan;Huang Li-Yuan
期刊：
中国物理B ,2010年19(3):55-60 ISSN：1674-1056
通讯作者：
Fang Mao-Fa
作者机构：
[Kang Guo-Dong; Ouyang Xi-Cheng; Deng Xiao-Juan; Fang Mao-Fa] Hunan Normal Univ, Coll Phys & Informat Sci, Changsha 410081, Hunan, Peoples R China.;[Ouyang Xi-Cheng] Hunan Agr Univ, Coll Sci, Changsha 410128, Hunan, Peoples R China.;[Huang Li-Yuan] Changsha Univ, Dept Elect & Commun Engn, Changsha 410003, Hunan, Peoples R China.
通讯机构：
[Fang Mao-Fa] H;Hunan Normal Univ, Coll Phys & Informat Sci, Changsha 410081, Hunan, Peoples R China.
关键词：
entanglement sudden death;concurrence;two-photon Jaynes-Cummings model;Kerr-like medium
摘要：
In this paper, the entanglement dynamics of a double two-photon Jaynes-Cummings model with Kerr-like medium is investigated. It is shown that initial entanglement has an interesting subsequent time evolution, including the so-called entanglement sudden death effect. It is also shown analytically that the Kerr-like medium can repress entanglement sudden death and enhance the degree of atom-atom entanglement. A more interesting fact is that the Kerr effect is more obvious when each of the two cavities with have the Kerr-like medium than only one of them with the Kerr-like medium.
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英文
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Molecular characterization of Ran gene up-regulated in large yellow croaker (Pseudosciaena crocea) immunity
作者：
Han, Fang;Wang, Xiao-Qing;Yao, Cui-luan;Wang, Zhi-yong*
期刊：
Fish & Shellfish Immunology ,2010年29(2):327-333 ISSN：1050-4648
通讯作者：
Wang, Zhi-yong
作者机构：
[Wang, Zhi-yong; Han, Fang; Wang, Xiao-Qing] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Yao, Cui-luan; Wang, Zhi-yong; Han, Fang] Jimei Univ, Fisheries Coll, Key Lab Sci & Technol Aquaculture & Food Safety, Xiamen 361021, Peoples R China.
通讯机构：
[Wang, Zhi-yong] H;Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.
关键词：
Immunity;Large yellow croaker;RanGTPase;Real-time PCR;Up-regulated expression
摘要：
RanGTPase, one family of small G protein superfamily, has been widely demonstrated to be involved in transport system between cytoplasm and nucleus. However the knowledge about the function of RanGTPase in immunity remains limited. In this report, Ran gene (named LycRan) cDNA was cloned from the large yellow croaker, Pseudosciaena crocea, a marine fish. The full-length cDNA of LycRan was of 1033 bp, including a 5'-terminal untranslated region (UTR) of 43 bp, 3'-terminal UTR of 338 bp and an open reading frame (ORF) of 648 bp encoding a polypeptide of 216 amino acids. The deduced protein is highly homologous, it shares 90.74%, 88.89%, 89.35% and 85.20% identities with those of salmon, frog, human and fruit fly respectively. RT-PCR analysis indicated that LycRan gene was constitutively expressed in 9 tissues examined, including kidney, liver, gill, muscle, spleen, skin, heart, intestine and blood. The result of quantitative Real-Time RT-PCR analysis revealed the highest expression in kidney and the weakest expression in skin. Time course analysis showed that LycRan expression was obviously up-regulated in kidney, blood and spleen after immunization with either poly I:C or formalin-inactive Gram-negative bacterium Vibrio parahaemolyticus. It indicated that the highest expression was 2.8 times (at 48 h) as much as that in the control in the kidney (p < 0.05) challenged by poly I:C and 3.2 times (at 24 h) in the blood (p < 0.05) challenged by bacteria. These results suggested that LycRan might play an important role in large yellow croaker defense against the pathogen infection. Our study, therefore, might provide a clue to elucidate the large yellow croaker innate immunity. © 2010.
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英文
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Concentration of components in Danggui-Buxue Decoction by macroporous resin chromatography guided with LC
作者：
Dong, Xinrong;Liang, Yizeng* ;Wang, Bing
期刊：
Chromatographia ,2010年72(7-8):725-730 ISSN：0009-5893
通讯作者：
Liang, Yizeng
作者机构：
[Dong, Xinrong; Liang, Yizeng; Wang, Bing] Cent S Univ, Res Ctr Modernizat Chinese Med, Coll Chem & Chem Engn, Changsha 410083, Peoples R China.;[Dong, Xinrong] Hunan Agr Univ, Coll Sci, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liang, Yizeng] C;Cent S Univ, Res Ctr Modernizat Chinese Med, Coll Chem & Chem Engn, Changsha 410083, Peoples R China.
关键词：
Column liquid chromatography;Concentration of bioactive components;Astragalus membranaceus;Angelicae sinensis
摘要：
A method for simultaneous concentration of multiple bioactive components (MBCs) in Danggui-Buxue Decoction (DBD) was comprehensively investigated in this work. The column chromatography with macroporous resin ADS-7 as an absorbent was used for concentration of the MBCs in DBD under the guidance of liquid chromatography (LC). After one-run treatment with the resin, the contents of major flavonoids reached 18.3 and 13.9% in the eluting fraction of 50 and 70% ethanol, which were 177.2 and 134.6 times higher than those in DBD crude extract. The contents of main saponins reached 8.7 and 9.9% in the eluting fractions of 10 and 30% ethanol, which were 32.8 and 37.3 times higher than those in DBD crude extract; and ferulic acid and ligustilide were also enriched in the eluting fractions of 10 and 70% ethanol. © 2010 Vieweg+Teubner Verlag | Springer Fachmedien Wiesbaden GmbH.
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英文
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Determination of both jasmonic acid and methyl jasmonate in plant samples by liquid chromatography tandem mass spectrometry
作者：
Liu Xia;Yang Yanli;Lin WanHuang;Tong JianHua;Huang ZhiGang;...
期刊：
科学通报(英文版) ,2010年55(21):2231-2235 ISSN：2095-9273
通讯作者：
Xiao LangTao
作者机构：
[Xiao LangTao; Lin WanHuang; Tong JianHua; Huang ZhiGang; Yang Yanli; Liu Xia] Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Xiao LangTao] H;Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha 410128, Hunan, Peoples R China.
关键词：
jasmonic acid;methyl jasmonate;extraction;purification;LC-MS-MS
摘要：
An efficient method for the simultaneous extraction and quantification of both jasmonic acid (JA) and methyl jasmonate (MeJA) from a single plant sample was developed. Plant tissues were firstly extracted by using 100% cold methanol, then the Sep-pak C18 solid-phase extraction (SPE) cartridges were adopted for the purification of sample extract, and finally JA and MeJA were determined by the liquid chromatography tandem mass spectrometry (LC-MS-MS) system. It was found that the accuracy was improved by using the extra standard for the estimation and correction of JA and MeJA losses in the extraction and purification process. The detection limits for JA and MeJA were 0.03 and 0.075 ng mL-1, respectively, and the average recovery rate of JA and MeJA was 92.48% and 94.30%. The method was found to be reproducible and selective, yielding well isolated and easily detectable peaks for both JA and MeJA and simplifying the time-consuming extraction and purification. High-performance liquid chromatography (HPLC) system was employed as the control detection method for the LC-MS-MS system. The two systems were compared in their specificity and accuracy. © 2010 Science China Press and Springer-Verlag Berlin Heidelberg.
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英文
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Surface self-diffusion of a Pt adatom on cuboctahedral and truncated decahedral clusters, size dependence
作者：
Yang, J. Y.* ;Hu, W. Y.;Tang, J. F.
期刊：
EUROPEAN PHYSICAL JOURNAL B ,2010年78(3):315-321 ISSN：1434-6028
通讯作者：
Yang, J. Y.
作者机构：
[Yang, J. Y.] Hunan Inst Engn, Dept Math & Phys, Xiangtan 411104, Peoples R China.;[Hu, W. Y.] Hunan Univ, Dept Appl Phys, Changsha 410082, Hunan, Peoples R China.;[Tang, J. F.] Hunan Agr Univ, Dept Appl Phys, Changsha 410028, Hunan, Peoples R China.
通讯机构：
[Yang, J. Y.] H;Hunan Inst Engn, Dept Math & Phys, Xiangtan 411104, Peoples R China.
关键词：
The European Physical Journal B;Condensed Matter;Complex Systems;journal;EPJ
摘要：
The self-diffusion of single Pt adatom on the surface of cuboctahedral and truncated decahedral clusters with 561-10179 atoms are studied within the context of the many-body potentials obtained via the embedded atom method. The minimum energy diffusion path and the corresponding energy barrier for adatom diffusion on the cuboctahedral and truncated decahedral clusters surfaces are determined through a combination of the quenched molecular dynamics and the nudged elastic band method. The calculated energy barriers are consistent with the available experimental data. The dependence of energy barrier for adatom diffusion across the step edge on the cluster size is found. For the larger cuboctahedral and truncated decahedral clusters, the simulations show that the movement of the adatom is confined to a central region, and the adatom may escape from the center region only at elevated temperatures. In addition, we also find that the truncated decahedral structure is more favorable over the cuboctahedral structure for smaller clusters. The cluster growth experiments support our results. © 2010 EDP Sciences, SIF, Springer-Verlag Berlin Heidelberg.
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英文
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MicroRNA Discovery and Analysis of Pinewood Nematode Bursaphelenchus xylophilus by Deep Sequencing
作者：
Huang, Qi-Xing* ;Cheng, Xin-Yue;Mao, Zhen-Chuan;Wang, Yun-Sheng;Zhao, Li-Lin;...
期刊：
PLOS ONE ,2010年5(10):e13271 ISSN：1932-6203
通讯作者：
Huang, Qi-Xing
作者机构：
[Yan, Xia; Cheng, Xin-Yue; Huang, Qi-Xing; Xu, Ru-Mei] Beijing Normal Univ, Coll Life Sci, Beijing 100875, Peoples R China.;[Xie, Bing-Yan; Mao, Zhen-Chuan; Huang, Qi-Xing; Wang, Yun-Sheng] Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing, Peoples R China.;[Wang, Yun-Sheng] Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha, Hunan, Peoples R China.;[Zhao, Li-Lin] Chinese Acad Sci, Inst Zool, State Key Lab Integrated Management Pest Insects, Beijing, Peoples R China.;[Ferris, Virginia R.] Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA.
通讯机构：
[Huang, Qi-Xing] B;Beijing Normal Univ, Coll Life Sci, Beijing 100875, Peoples R China.
关键词：
MicroRNAs;RNA sequencing;cDNA libraries;Expressed sequence tags;RNA stem-loop structure;Caenorhabditis elegans;Heat shock response;Gene expression
摘要：
Background: MicroRNAs (miRNAs) are considered to be very important in regulating the growth, development, behavior and stress response in animals and plants in post-transcriptional gene regulation. Pinewood nematode, Bursaphelenchus xylophilus, is an important invasive plant parasitic nematode in Asia. To have a comprehensive knowledge about miRNAs of the nematode is necessary for further in-depth study on roles of miRNAs in the ecological adaptation of the invasive species. Methods and Findings: Five small RNA libraries were constructed and sequenced by Illumina/Solexa deep-sequencing technology. A total of 810 miRNA candidates (49 conserved and 761 novel) were predicted by a computational pipeline, of which 57 miRNAs (20 conserved and 37 novel) encoded by 53 miRNA precursors were identified by experimental methods. Ten novel miRNAs were considered to be species-specific miRNAs of B. xylophilus. Comparison of expression profiles of miRNAs in the five small RNA libraries showed that many miRNAs exhibited obviously different expression levels in the third-stage dispersal juvenile and at a cold-stressed status. Most of the miRNAs exhibited obviously down-regulated expression in the dispersal stage. But differences among the three geographic libraries were not prominent. A total of 979 genes were predicted to be targets of these authentic miRNAs. Among them, seven heat shock protein genes were targeted by 14 miRNAs, and six FMRFamide-like neuropeptides genes were targeted by 17 miRNAs. A real-time quantitative polymerase chain reaction was used to quantify the mRNA expression levels of target genes. Conclusions: Basing on the fact that a negative correlation existed between the expression profiles of miRNAs and the mRNA expression profiles of their target genes (hsp, flp) by comparing those of the nematodes at a cold stressed status and a normal status, we suggested that miRNAs might participate in ecological adaptation and behavior regulation of the nematode. This is the first description of miRNAs in plant parasitic nematodes. The results provide a useful resource for further in-depth study on molecular regulation and evolution of miRNAs in plant parasitic nematodes. © 2010 Huang et al.
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英文
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Dissipation and Evaluation of Hexaflumuron Residues in Chinese Cabbage Grown in Open Fields
作者：
Huang, Fan* ;Guo, Zhengyuan;Chen, Ling;Xu, Zhen;Liu, Ying;...
期刊：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ,2010年58(8):4839-4843 ISSN：0021-8561
通讯作者：
Huang, Fan
作者机构：
[Liu, Ying; Yang, Changming; Xu, Zhen; Huang, Fan; Chen, Ling] Tongji Univ, Coll Environm Sci & Engn, State Key Lab Pollut Control & Resources Reuse, Shanghai 200092, Peoples R China.;[Guo, Zhengyuan] Hunan Agr Univ, Inst Agroenvironm Protect, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Huang, Fan] T;Tongji Univ, Coll Environm Sci & Engn, State Key Lab Pollut Control & Resources Reuse, Shanghai 200092, Peoples R China.
关键词：
Hexaflumuron;Chinese cabbage;soil;residue behavior;preharvest interval (PHI);food safety
摘要：
The dissipation and residue behavior of hexaflumuron in Chinese cabbage (Brassica pekinensis) under different treatments were investigated at two main cabbage-growing areas in China. The dissipation rates of hexaflumuron in cabbages and soils fit a first-order decay process very well. The dissipation times for 50% of the hexaflumuron in cabbage and soil were 3.37 and 3.01 days from Hunan province and 5.58 and 3.68 days in Tianjin, respectively, when Chinese cabbage was treated with 180 g of active ingredient (ai)/ha. Hexaflumuron residual times in cabbage and soil were influenced strongly by the application rate and frequency. The application regimen of hexaflumuron in the cabbage field, a rate of 120 g of ai/ha with two applications at a 7 day interval and a 21 day preharvest interval, was recommended from the point of view of ensuring food safety. The residual levels of hexaflumuron in cabbages were lower than the standards of the "Positive List System for Agricultural Chemical Residues in Foods" based on the recommended application regimen. © 2010 American Chemical Society.
语种：
英文
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Reduction of unproductive tillers did not increase the grain yield of irrigated rice
作者：
Ao, Hejun;Peng, Shaobing* ;Zou, Yingbin;Tang, Qiyuan;Visperas, Romeo M.
期刊：
Field Crops Research ,2010年116(1-2):108-115 ISSN：0378-4290
通讯作者：
Peng, Shaobing
作者机构：
[Ao, Hejun; Zou, Yingbin; Tang, Qiyuan] Hunan Agr Univ, Crop Physiol Ecol & Prod Ctr, Changsha 410128, Hunan, Peoples R China.;[Peng, Shaobing; Visperas, Romeo M.] Int Rice Res Inst, Crop & Environm Sci Div, Manila, Philippines.;[Peng, Shaobing] Int Rice Res Inst, Crop & Environm Sci Div, DAPO Box 7777, Manila, Philippines.
通讯机构：
[Peng, Shaobing] I;Int Rice Res Inst, Crop & Environm Sci Div, DAPO Box 7777, Manila, Philippines.
关键词：
Biomass production;Grain yield;Tiller removal;Unproductive tillers;Yield components
摘要：
Tillering ability is one of the most important traits of the rice plant because it plays a major role in determining grain yield. However, it is not clear whether rice yield potential could be further increased if unproductive tillers were reduced or eliminated. Two field experiments were conducted to determine the effects of tiller reduction on plant growth and grain yield in two varieties with different tillering ability. Two methods were used to reduce tillers: the first was the removal of tillers manually at different crop growth stages and the second was to grow rice plants through holes in styrofoam slats, which provided a physical restriction on tiller emergence. Tiller number declined by 12-57% with the first method and by 18-47% with the second method compared with the control. Despite the significant reduction in tiller number and unproductive tiller percentage, none of the treatments with manual tiller removal and physical restriction of tillering resulted in a significant increase in grain yield compared with the control. Decreases in leaf area and biomass at heading and panicle number at maturity were observed when tiller number declined significantly, but grain yield was not necessarily reduced because it was compensated for by more biomass production after heading and increased spikelet number per panicle. The two varieties with different tillering ability had similar responses to tiller reduction. Based on the two methods of tiller reduction used in this study, we conclude that a further increase in rice yield potential may not be possible by reducing or eliminating unproductive tillers. © 2009 Elsevier B.V. All rights reserved.
语种：
英文
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A taxonomic revision of Miscanthus s.l. (Poaceae) from China
作者：
Sun, Qian;Lin, Qi* ;Yi, Zi-Li;Yang, Zhi-Rong;Zhou, Fa-Song
期刊：
Botanical Journal of the Linnean Society ,2010年164(2):178-220 ISSN：0024-4074
通讯作者：
Lin, Qi
作者机构：
[Sun, Qian; Lin, Qi; Yang, Zhi-Rong] Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China.;[Sun, Qian] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China.;[Yi, Zi-Li] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Zhou, Fa-Song] Mendel Biotechnol Inc, Hayward, CA 94545 USA.
通讯机构：
[Lin, Qi] C;Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China.
关键词：
energy;new combination;new state;plant taxonomy;statistical analysis
摘要：
On the basis of morphological examination, field investigation, observation of cultivated accessions and statistical analysis, the genus Miscanthus s.l. (Poaceae) from China was taxonomically revised. Two subgenera (Miscanthus subgenus Miscanthus and Miscanthus subgenus Diandranthus), two sections [Miscanthus subgenus Miscanthus section Miscanthus and Miscanthus subgenus Miscanthus section Triarrhena (Maximowicz) Honda], six species, two subspecies and four varieties in this genus were recognized in this report. Miscanthus sacchariflorus ssp. lutarioriparius and Miscanthus nudipes var. yunnanensis were also recognized. A key to the taxa of Miscanthus from China is provided. A morphological description, distribution, and habit and phenology are summarized for each species. Distribution maps and morphological illustrations of each species are also provided. © 2010 The Linnean Society of London, Botanical Journal of the Linnean Society, 2010, 164, 178–220.
语种：
英文
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Effects of dietary inclusion of soy oil on growth performance, carcass characteristics, serum metabolites, hormones and meat quality in finishing pigs
作者：
Fang, Jun;Xu, Hai-Jun;Liu, Zhi-Qiang;Gu, Wan-ting;Tang, Bi-E;...
期刊：
Journal of Food, Agriculture and Environment ,2010年8(3-4):759-766 ISSN：1459-0255
通讯作者：
Liu, Zhiqiang
作者机构：
[Fang, Jun] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410125, Hunan, Peoples R China.;[Gu, Wan-ting; Tang, Bi-E; Liu, Zhiqiang; Xu, Hai-Jun; Liu, Zhi-Qiang] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China.;[Xu, Hai-Jun] W Anhui Univ, Dept Chem & Life Sci, Luan 237012, Anhui, Peoples R China.;[Yin, Ronghua; Wang, Yongfei] Twins Grp Co Ltd, Ctr Res & Dev, Nanchang 330031, Jiangxi, Peoples R China.;[Fan, M. Z.] Univ Guelph, Dept Anim & Poultry Sci, Guelph, ON N1G 2W15, Canada.
通讯机构：
[Liu, Zhiqiang] C;Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China.
关键词：
Pig;digestible energy;soy oil;linoleic acid;intramuscular fat;fatty acids
摘要：
High levels of polyunsaturated fatty acids (PUFA) in pork are beneficial and saturated fatty acids (SFA) detrimental to human health. Inclusion of PUFA in diet may decrease the amount of intramuscular fat (IMF) due to inhibition of de nova synthesis of fatty acids, which has a negative effect on meat eating quality. Diet energy level is a leading factor for lipid deposition in IMF. We hypothesize that increasing dietary energy level by including PUFA will increase its content without decreasing IMF in meat, thus increasing meat's nutritional value without lowering meat eating quality. The results of this study showed that increasing dietary energy level by inclusion of soy oil decreased average daily feed intake (ADFI) (P<0.01) and feed:gain ratio (P<0.01), increased average daily weight gain ADG (P<0.05) and serum triglyceride concentration (P<0.05) and decreased serum urea concentration (P<0.05). Carcass characteristics, meat quality and serum concentrations of hormones [insulin, growth hormone (GH), insulin-like growth factor-I (IGF-I), glucagon and tumor necrosis factor-alpha (TNF-alpha)] were unaffected. Percentages of SFA and monounsaturated fatty acids (MUFA) were decreased while percentages of PUFA were increased (P<0.05) in longissimus dorsi (LD) muscle as dietary energy level increased. The results of this study suggested that increasing dietary energy level by inclusion of soy oil could be a suitable way to improve growth performance as well as nutritional value of pork without lowering eating quality.
语种：
英文
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Expression of ethylene response factor JERF1 in rice improves tolerance to drought
作者：
Zhang, Zhijin;Li, Fang;Li, Dingjun;Zhang, Haiwen;Huang, Rongfeng*
期刊：
Planta ,2010年232(3):765-774 ISSN：0032-0935
通讯作者：
Huang, Rongfeng
作者机构：
[Zhang, Zhijin; Li, Fang; Zhang, Haiwen; Huang, Rongfeng] Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China.;[Li, Fang; Li, Dingjun] Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.;[Zhang, Zhijin; Zhang, Haiwen; Huang, Rongfeng] Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China.;[Zhang, Zhijin; Zhang, Haiwen; Huang, Rongfeng] Natl Ctr Plant Gene Res Beijing, Beijing 100081, Peoples R China.
通讯机构：
[Huang, Rongfeng] C;Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China.
关键词：
Abscisic acid;Drought;ERF protein;Oryza sativa;Transcriptional regulation
摘要：
Ethylene response factor (ERF) proteins regulate a variety of stress responses in plant. JERF1, a tomato ERF protein, can be induced by abscisic acid (ABA). Overexpression of JERF1 enhanced the tolerance of transgenic tobacco to high salt concentration, osmotic stress, and low temperature by regulating the expression of stress-responsive genes by binding to DRE/CRT and GCC-box cis-elements. In this research, we further report that overexpression of JERF1 significantly enhanced drought tolerance of transgenic rice. The overexpression activated the expression of stress-responsive genes and increased the synthesis of the osmolyte proline by regulating the expression of OsP5CS, encoding the proline biosynthesis key enzyme deltal-pyrroline-5-carboxylate synthetase. JERF1 also activated the expression of two ABA biosynthesis key enzyme genes, OsABA2 and Os03g0810800, and increased the synthesis of ABA in rice. Analysis of cis-elements of JERF1-targeted genes pointed to the existence of DRE/CRT and/or GCC box in their promoters, indicating that JERF1 could activate the expression of related genes in rice by binding to these cis-elements. Unlike some other ERF proteins, constructive overexpression of JERF1 did not change the growth and development of transgenic rice, which makes JEFR1 a potentially useful source in breeding for greater tolerance to abiotic stress.
语种：
英文
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Effect of reactant phase form on the properties of LiFePO4 synthesized by carbothermal reduction method
作者：
Zhong, Mei-e* ;Zhou, Zhentao
期刊：
Solid State Ionics ,2010年181(35-36):1607-1610 ISSN：0167-2738
通讯作者：
Zhong, Mei-e
作者机构：
[Zhong, Mei-e] Hunan Agr Univ, Coll Sci, Changsha 410128, Peoples R China.;[Zhou, Zhentao] S China Univ Technol, Coll Mat Sci & Engn, Guangzhou 510640, Guangdong, Peoples R China.
通讯机构：
[Zhong, Mei-e] H;Hunan Agr Univ, Coll Sci, Changsha 410128, Peoples R China.
关键词：
Carbothermal reduction method;Cathode;LiFePO4;Lithium ion batteries
摘要：
Pure olivine LiFePO<inf>4</inf>/C composite compounds are synthesized by carbothermal reduction (CTR) method using trigonal anhydrous FePO<inf>4</inf> raw material. The physical and electrochemical properties of the prepared samples are investigated in comparison with those of a sample obtained from incompletely crystallized hydrous FePO<inf>4</inf>•2H<inf>2</inf>O. The olivine LiFePO<inf>4</inf>/C prepared with trigonal anhydrous FePO<inf>4</inf> has a high discharge capacity of 142 mAh g<sup>- 1</sup> at 0.1 C. The composite also displays a better rate capability, a higher discharge capacity and a more stable cycle-life than that synthesized with incompletely crystallized hydrous FePO<inf>4</inf>•2H<inf>2</inf>O. The improved electrochemical performance of trigonal anhydrous FePO<inf>4</inf> synthesis samples originates mainly from the single-phase structure and small particle size. ©2010 Elsevier B.V. All rights reserved.
语种：
英文
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The molecular mechanisms of male reproductive organogenesis in rice (Oryza sativa L.)
作者：
Tian, Yun;Yang, Hui;Zhang, Hai Wen;Dai, Qian;Fang, Jun;...
期刊：
Plant Growth Regulation ,2010年61(1):11-20 ISSN：0167-6903
通讯作者：
Lu, Xiang Yang
作者机构：
[Tian, Yun; Dai, Qian; Lu, Xiang Yang; Yang, Hui; Fang, Jun] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Tian, Yun; Dai, Qian; Lu, Xiang Yang; Yang, Hui; Fang, Jun] Hunan Agr Bioengn Res Inst, Changsha 410128, Hunan, Peoples R China.;[Tian, Yun; Qing, Xian Guo] Yuan Long Ping High Tech Agr CO LTD, Changsha 410001, Hunan, Peoples R China.;[Qing, Xian Guo] Natl Hybrid Rice Res & Dev Ctr, Changsha 410125, Hunan, Peoples R China.;[Zhang, Hai Wen] Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China.
通讯机构：
[Lu, Xiang Yang] H;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
关键词：
Stamen;Anther;Male sterility;Oryza sativa L.
摘要：
Stamen is the male reproductive organ of rice (Oryza sativa L.), and the development is a considerably significant stage during the sexual reproduction. It is very important to reveal what is the molecular mechanisms which controlling the development of stamen, and will be helpful in producing hybrid seeds by manipulating the male sterility. This article reviews the progress on the molecular mechanisms of male reproductive organogenesis in rice, which would facilitate the further studies on male sterile genes in rice.
语种：
英文
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Responses of Synechocystis sp. PCC 6803 (cyanobacterium) photosystemⅡto pyrene stress
作者：
Shao, Jihai;Yu, Gongliang;Wu, Zhongxing;Peng, Xin;Li, Renhui*
期刊：
环境科学学报(英文版) ,2010年22(7):1091-1095 ISSN：1001-0742
通讯作者：
Li, Renhui
作者机构：
[Shao, Jihai; Yu, Gongliang; Wu, Zhongxing; Li, Renhui; Peng, Xin] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.;[Shao, Jihai] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China.;[Shao, Jihai] Hunan Agr Univ, Coll Resources & Environm, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Li, Renhui] C;Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
关键词：
PSBA;pyrene;Synechocystis;toxicity;photosystem Ⅱ
摘要：
In order to explore the mechanism of acute toxicity for pyrene to cyanobacterial organisms, the responses of Synechocystis sp. PCC 6803 photosystem II (PS II) under pyrene stress were studied. The results showed there was no significant difference about the oxygen evolution under 0.125 mg/L pyrene stress when compared with control, but it was significantly lower than control at 0.625 mg/L pyrene. Polyphasic chlorophyll-a fluorescence transients in cells of Synechocystis sp. PCC 6803 exhibited a typical increase including O, J, I, and P phases. Fluorescence yield at phases J, I and P declined slightly at 0.125 and 0.625 mg/L pyrene, and significantly lower than control at 3.125 mg/L. According to the parameters deviated from JIP-test, no modification was induced by pyrene both at the donor side and at the acceptor side of PS II, and the reaction centre of PS II is the primary damaging target. Based on the expressing of four key genes (psbA, psbB, psbC and psbO) of PS II, only psbA showed significant difference at 3.125 mg/L pyrene when compared with control.
语种：
英文
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