Cloning and characterization of the drought-resistance OsRCI2-5 gene in rice (Oryza sativa L.)
作者：
Li, L.;Li, N.;Song, S. F.;Li, Y. X.;Xia, X. J.;...
期刊：
Genetics and Molecular Research ,2014年13(2):4022-4035 ISSN：1676-5680
通讯作者：
Deng, H. F.
作者机构：
[Song, S. F.; Deng, H. F.; Li, N.; Fu, X. Q.; Li, L.] Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha, Hunan, Peoples R China.;[Xia, X. J.] Chinese Acad Sci, Inst Subtrop Agr, Changsha, Hunan, Peoples R China.;[Chen, G. H.; Li, Y. X.] Hunan Agr Univ, Coll Agr, Changsha, Hunan, Peoples R China.
通讯机构：
[Deng, H. F.] H;Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha, Hunan, Peoples R China.
关键词：
Drought resistance;Drought-resistance mechanisms;OsRCI2-5;Rice (Oryza sativa L.)
摘要：
The genomic expression profile of the super-hybrid rice Liangyoupeijiu female parent Pei'ai 64S in different tissues at different developmental stages under low temperature, drought, and high temperature stresses were detected using an Affymetrix GeneChip Rice Genome Array to screen upregulated and downregulated genes. In this study, we screened the drought-resistant gene OsRCI2-5, after which a constitutive OsRCI2-5 construct was created and transferred into Nipponbare. After polyethylene glycol-6000 and drought treatment, we found that the OsRCI2-5 gene improved the drought resistance of Nipponbare. Gene expression profiling showed that the OsRCI2-5 gene was expressed in the rice leaves, stems, and flower organs. Subcellular localization revealed that the gene was located in the membranes, and hence, we can deduce that a membrane signal peptide was responsible Characterization of a rice gene for drought resistance for signal transduction. © FUNPEC-RP.
语种：
英文
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Integrated nutrient, water and other agronomic options to enhance rice grain yield and N use efficiency in double-season rice crop
作者：
Qin, Jianquan;Impa, S. M.;Tang, Qiyuan* ;Yang, Shenghai;Yang, Jian;...
期刊：
Field Crops Research ,2013年148:15-23 ISSN：0378-4290
通讯作者：
Tang, Qiyuan
作者机构：
[Qin, Jianquan; Yang, Jian; Tang, Qiyuan; Tao, Yousheng] Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.;[Jagadish, Krishna S. V.; Qin, Jianquan; Impa, S. M.] Int Rice Res Inst, Crop & Environm Sci Div, Manila, Philippines.;[Yang, Shenghai] Extens Stn Qiandongnan State Agron Technol, Kaili 556000, Peoples R China.
通讯机构：
[Tang, Qiyuan] H;Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.
关键词：
Agronomic management;Grain yield;Nitrogen fertilizer use efficiency;Nitrogen partitioning;Radiation use efficiency;Super rice hybrid
摘要：
Optimizing resource use options to achieve high NUE and grain yield.Large sink size in super rice hybrids disadvantage under short grain-filling duration.Post-flowering wetting and drying enhanced N and assimilate partitioning into grains."Climatic yield potential" over "actual attained yield" a realistic estimate. Options to increase resource use efficiency and climatic yield potential of locally adapted super rice hybrids including combined water, nutrient and other agronomic management are limited. Hence, the aim of our three-year (six seasons) experiments during early-season (ESR; Luliangyou996) and late-season (LSR; C-liangyou396) rice in southern China was to identify key yield parameters and optimum resource use options to enhance the crop's climatic yield potential. Grain yield averaged across all three years with effective N management combined with post-anthesis shallow wetting and drying was 32.8% and 37.1% higher than the normal farmers' practice in Liuyang County in ESR and LSR, respectively. More spikeletsm-2 were the key to achieving high yield potential, further supported by increased leaf area index and high radiation interception and internal use efficiency. The split application of nitrogen in combination with shallow wetting and drying allowed for better N uptake, use efficiency and partitioning, leading to enhanced biomass and yield. The high yield potential, however, was not just a function of genetics and management but also depended on the climatic conditions prevailing, particularly temperature and radiation. In ESR, lower temperature during vegetative stage reduced overall biomass and sink size while subsequent higher temperature reduced the total grain filling period by 17 days compared with LSR, indicating a climatic condition-driven decline in yield potential rather than lower genetic potential of the super hybrids. A lack of correlation of spikeletspanicle-1 and spikeletsm-2 with grain-filling percentage in LSR provided evidence that a larger sink does not necessarily result in poor grain filling when sufficient time and assimilates for grain filling are provided, which is more climate dependent. Our work highlights the benefits of integrating nutrient, water and agronomic management options to achieve high NUE and grain yield. © 2013 Elsevier B.V.
语种：
英文
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Recent Progress in Understanding PAMP- and Effector-Triggered Immunity against the Rice Blast Fungus Magnaporthe oryzae
作者：
Liu, Wende;Liu, Jinling;Ning, Yuese;Ding, Bo;Wang, Xuli;...
期刊：
分子植物 ,2013年6(3):605-620 ISSN：1674-2052
通讯作者：
Wang, Guo-Liang
作者机构：
[Wang, Guo-Liang; Liu, Jinling; Wang, Xuli; Liu, Wende; Ning, Yuese; Ding, Bo] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China.;[Wang, Zhilong; Liu, Jinling] Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China.;[Wang, Zhilong; Liu, Jinling] Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.;[Wang, Guo-Liang] Ohio State Univ, Dept Plant Pathol, Columbus, OH 43210 USA.
通讯机构：
[Wang, Guo-Liang] C;Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China.
关键词：
innate immunity;PTI;ETI;rice;Magnaporthe oryzae
摘要：
Rice blast, caused by the fungal pathogen Magnaporthe oryzae, is one of the most destructive diseases of rice worldwide. The rice-M. oryzae pathosystem has become a model in the study of plant-fungal interactions because of its scientific advancement and economic importance. Recent studies have identified a number of new pathogen-associated molecular patterns (PAMPs) and effectors from the blast fungus that trigger rice immune responses upon perception. Interaction analyses between avirulence effectors and their cognate resistance proteins have provided new insights into the molecular basis of plant-fungal interactions. In this review, we summarize the recent research on the characterization of those genes in both M. oryzae and rice that are important for the PAMP- and effector-triggered immunity recognition and signaling processes. We also discuss future directions for research that will further our understanding of this pathosystem.
语种：
英文
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Tillering dynamics of hybrid rice in tropical and sub-tropical environments
作者：
Md, Ibrahim* ;Huang, Min;Jiang, Peng;Adil, Badshah;Tang, Qiyuan;...
期刊：
Research on Crops ,2013年14(1):15-27 ISSN：0972-3226
通讯作者：
Md, Ibrahim
作者机构：
[Md, Ibrahim; Adil, Badshah; Tang, Qiyuan; Zou, Yingbin; Jiang, Peng; Huang, Min] Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Md, Ibrahim] H;Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.
关键词：
Hill dry weight;hybrid rice;panicle number;stem dry weight;tillering duration;tillering rate
摘要：
To compare the tillering pattern of hybrid rice, multiple field experiments were conducted involving three hybrid rice cultivars (HS 995, HS 48 and LYPJ) with three nitrogen fertilizer levels during 2009 to 2011. The experimental sites were selected in the sub-tropical environments of Changsha, Hunan Province in China, and in tropical climate zones of Habiganj and Gazipur in Bangladesh. All parameters of this study were significantly influenced by the locations and mostly the cultivars as well as the N levels. Tests confirmed that (i) panicle number was highest in Hunan and LYPJ showed the superiority even under cross ecosystem. The higher panicle number was the consequential of higher tiller number in both the environments; (ii) tillering rate (TR) responded to higher tillering capability; (iii) lower tiller emergence resulted in low panicle number but showed the higher panicle production efficiency in HS 48 and vice versa in LYPJ; (iv) early tiller emergence at Hunan showed the advantage to higher panicle number; (v) dry weight of stem and hill was more than 60% higher in Hunan and 23% higher in Habiganj over Gazipur site and (vi) higher N dose generated significant increases in hill dry matter, higher tiller number as well as increased the panicle number. Our study suggested that selection should be emphasized on cultivars those boast of more potential to high TR and to produce more dry matter especially at early growth period even under low temperatures in a wider area basis adaptation.
语种：
英文
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Source-sink dynamics and proteomic reprogramming under elevated night temperature and their impact on rice yield and grain quality
作者：
Shi, Wanju;Muthurajan, Raveendran;Rahman, Hifzur;Selvam, Jagadeesh;Peng, Shaobing;...
期刊：
New Phytologist ,2013年197(3):825-837 ISSN：0028-646X
通讯作者：
Jagadish, Krishna S. V.
作者机构：
[Zou, Yinbin; Shi, Wanju] Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.;[Jagadish, Krishna S. V.; Shi, Wanju] IRRI, Crop & Environm Sci Div, Manila, Philippines.;[Rahman, Hifzur; Muthurajan, Raveendran; Selvam, Jagadeesh] Tamil Nadu Agr Univ, Ctr Plant Mol Biol, Coimbatore 641003, Tamil Nadu, India.;[Peng, Shaobing] Huazhong Agr Univ, Coll Plant Sci & Technol, MOA Key Lab Crop Ecophysiol & Farming Syst Middle, Wuhan 430070, Hubei, Peoples R China.;[Jagadish, Krishna S. V.] IRRI, Crop & Environm Sci Div, DAPO Box 7777, Manila, Philippines.
通讯机构：
[Jagadish, Krishna S. V.] I;IRRI, Crop & Environm Sci Div, DAPO Box 7777, Manila, Philippines.
关键词：
flag leaf;grain filling;grain quality;high night temperature (HNT);nonstructural carbohydrate (NSC);proteome;rice (Oryza sativa);spikelets
摘要：
High night temperatures (HNTs) can reduce significantly the global rice (Oryza sativa) yield and quality. A systematic analysis of HNT response at the physiological and molecular levels was performed under field conditions. Contrasting rice accessions, N22 (highly tolerant) and Gharib (susceptible), were evaluated at 22°C (control) and 28°C (HNT). Nitrogen (N) and nonstructural carbohydrate (NSC) translocation from different plant tissues into grains at key developmental stages, and their contribution to yield, grain-filling dynamics and quality aspects, were evaluated. Proteomic profiling of flag leaf and spikelets at 100% flowering and 12 d after flowering was conducted, and their reprogramming patterns were explored. Grain yield reduction in susceptible Gharib was traced back to the significant reduction in N and NSC translocation after flowering, resulting in reduced maximum and mean grain-filling rate, grain weight and grain quality. A combined increase in heat shock proteins (HSPs), Ca signaling proteins and efficient protein modification and repair mechanisms (particularly at the early grain-filling stage) enhanced N22 tolerance for HNT. The increased rate of grain filling and efficient proteomic protection, fueled by better assimilate translocation, overcome HNT tolerance in rice. Temporal and spatial proteome programming alters dynamically between key developmental stages and guides future transgenic and molecular analysis targeted towards crop improvement. © 2012 New Phytologist Trust.
语种：
英文
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On-farm assessment of effect of low temperature at seedling stage on early-season rice quality
作者：
Huang, Min;Jiang, Ligeng;Zou, Yingbin* ;Zhang, Weixing
期刊：
Field Crops Research ,2013年141:63-68 ISSN：0378-4290
通讯作者：
Zou, Yingbin
作者机构：
[Jiang, Ligeng; Huang, Min] Guangxi Univ, Key Lab Crop Cultivat & Farming Syst, Nanning 530004, Peoples R China.;[Zou, Yingbin] Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.;[Zou, Yingbin] State Key Lab Hybrid Rice, Changsha 410125, Hunan, Peoples R China.;[Zhang, Weixing] China Natl Rice Res Inst, Hangzhou 310006, Zhejiang, Peoples R China.
通讯机构：
[Zou, Yingbin] H;Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.
关键词：
Growth period;Low temperature;Rice quality;Rice structure;Source capacity
摘要：
Low temperature often occurs at seedling stage in early rice-growing season in southern provinces of China. However, it is not clear whether the low temperature at seedling stage has an impact on early-season rice quality. This on-farm study was conducted to compare quality and growth traits of an early-season rice cultivar between two contrasting years with respect to temperature at seedling stage, 2009 (normal temperature) and 2010 (low temperature). The results showed that brown rice percentage, milled rice percentage, gelatinization temperature and gel consistency were significantly lower in 2010 than 2009, whereas 2010 had significantly higher percentage of chalky rice grains, degree of chalkiness and protein content than 2009. The yearly difference in rice quality was attributed to variation in rice structure. In 2010, a marked decrease of grain weight was observed, which was not accompanied by a decrease in rice length or width. Source capacity (aboveground biomass accumulation) did not explain the difference in grain weight between 2009 and 2010, because grain-filling rate was comparable in the two years. Shortened grain-filling duration, indirectly caused by the low temperature at seedling stage, was responsible for the decreased grain weight in 2010. These results suggest that low temperature at seeding stage can affect early-season rice quality through its indirect effect on grain-filling duration. © 2012 Elsevier B.V.
语种：
英文
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Genetic analysis and fine mapping of tms9, a novel thermosensitive genic male-sterile gene in rice (Oryza sativa L.)
作者：
Sheng, Zhonghua;Wei, Xiangjin;Shao, Gaoneng;Chen, Mingliang;Song, Jian;...
期刊：
Plant Breeding ,2013年132(2):159-164 ISSN：0179-9541
通讯作者：
Hu, Peisong
作者机构：
[Shao, Gaoneng; Luo, Ju; Wei, Xiangjin; Sheng, Zhonghua; Chen, Mingliang; Tang, Shaoqing; Hu, Peisong; Song, Jian] China Natl Rice Res Inst, Hangzhou 311400, Zhejiang, Peoples R China.;[Sheng, Zhonghua; Hu, Yichao; Chen, Liyun] Hunan Agr Univ, Rice Res Inst, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Hu, Peisong] C;China Natl Rice Res Inst, Hangzhou 311400, Zhejiang, Peoples R China.
会议名称：
中国作物学会2012年学术年会
会议时间：
2012-10-01
会议地点：
南昌
会议论文集名称：
中国作物学会2012年学术年会论文集
关键词：
thermosensitive genic male-sterile gene;tms9;fine mapping;rice
摘要：
Two-line hybrid rice as a novel hybrid breeding method has huge potential for yield increasing and utilization of intersubspecific heterosis, and it is of major significance for the food security of rice-consuming populations. Zhu1S is a thermosensitive genic male-sterile line of rice with low critical temperature and excellent combining ability, which has been widely exploited as a female parent in Chinese two-line hybrid rice breeding. Here, genetic mapping in F2 populations was used to show that its male sterility is inherited as a single recessive gene and that responsible gene (termed tms9) lies on the short arm of chromosome 2. A high-resolution linkage analysis which was based on the Zhu1S/R173 F2 population found that the thermosensitive genic male-sterile gene tms9 of Zhu1S was fine mapped between insertion-deletion (Indel) markers Indel 37 and Indel 57, and the genetic distance from the tms9 to the two markers was 0.12 and 0.31 cM, respectively. The physical distance between the two markers was about 107.2 kb. Sequence annotation databases showed that the two Indel markers (Indel 37 and Indel 57) were located on two BAC clones (B1307A11 and P0027A02). There are sixteen open reading frames (ORF) present in this region. The results of this study are of great significance for further cloning tms9 and molecular marker-assisted selection. © 2013 Blackwell Verlag GmbH.
语种：
英文
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Impact of temperature changes on early-rice productivity in a subtropical environment of China
作者：
Huang, Min;Zhang, Weixing;Jiang, Ligeng;Zou, Yingbin*
期刊：
Field Crops Research ,2013年146:10-15 ISSN：0378-4290
通讯作者：
Zou, Yingbin
作者机构：
[Jiang, Ligeng; Huang, Min] Guangxi Univ, Key Lab Crop Cultivat & Farming Syst, Nanning 530004, Peoples R China.;[Zhang, Weixing] China Natl Rice Res Inst, Hangzhou 310006, Zhejiang, Peoples R China.;[Zou, Yingbin] Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.;[Zou, Yingbin] State Key Lab Hybrid Rice, Changsha 410125, Hunan, Peoples R China.
通讯机构：
[Zou, Yingbin] H;Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.
关键词：
Climate warming;Low temperature events;Rice establishment method;Rice yield
摘要：
The impact of climate warming on rice production has attracted considerable attention. In this study, we (1) analyzed trends in maximum and minimum temperatures and low temperature events from 1980 to 2010 for early season, (2) evaluated relationships between rice-yield attributes and maximum or minimum temperature by using data from field experiments conducted in early seasons of 1991-2004, and (3) compared grain yield among different rice establishment methods by using data from field experiments done in early seasons of two contrasting years (2009 and 2010) with respect to low temperature events in Changde, Hunan Province, China. The results showed that maximum and minimum temperatures in early season increased by 2.2. °C and 2.1. °C, respectively, during the period 1980-2010 and there was a significant relationship between early-rice grain yield and maximum temperature. Grain yield increased by 7% for each 1. °C increase in growing-season maximum temperature. The increase in grain yield with maximum temperature was driven from the increased daily yield rather than growth duration. However, stabilizing growth duration was critical to overcome the potential negative impact of future climate warming on early-rice production. On the other hand, the climate warming did not lead to significant decreases in low temperature events. In the year with low temperature events, direct seeding produced less grain yield than transplanting and seedling throwing by 32% and 23%, respectively. Our study suggests that direct seeding is a high risk establishment method for early-rice production and seedling throwing may be a more satisfactory method of establishing early-rice under current climate change scenarios. © 2013 Elsevier B.V.
语种：
英文
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De Novo Transcriptome of Brassica juncea Seed Coat and Identification of Genes for the Biosynthesis of Flavonoids
作者：
Liu, Xianjun;Lu, Ying;Yuan, Yuhui;Liu, Shuyan;Guan, Chunyun;...
期刊：
PLOS ONE ,2013年8(8):e71110 ISSN：1932-6203
通讯作者：
Liu, Zhongsong
作者机构：
[Guan, Chunyun; Lu, Ying; Liu, Zhongsong; Liu, Xianjun; Liu, Shuyan; Chen, Sheyuan; Yuan, Yuhui] Hunan Agr Univ, Oilseed Crops Inst, Changsha, Hunan, Peoples R China.
通讯机构：
[Liu, Zhongsong] H;Hunan Agr Univ, Oilseed Crops Inst, Changsha, Hunan, Peoples R China.
关键词：
Brassica;Seed coat;Biosynthesis;Transcriptome analysis;Seeds;RNA sequencing;Arabidopsis thaliana;Sequence alignment
摘要：
Brassica juncea, a worldwide cultivated crop plant, produces seeds of different colors. Seed pigmentation is due to the deposition in endothelial cells of proanthocyanidins (PAs), end products from a branch of flavonoid biosynthetic pathway. To elucidate the gene regulatory network of seed pigmentation in B. juncea, transcriptomes in seed coat of a yellow-seeded inbred line and its brown-seeded near- isogenic line were sequenced using the next-generation sequencing platform Illumina/Solexa and de novo assembled. Over 116 million high-quality reads were assembled into 69,605 unigenes, of which about 71.5% (49,758 unigenes) were aligned to Nr protein database with a cut-off E-value of 10(-5). RPKM analysis showed that the brown-seeded testa up-regulated 802 unigenes and down-regulated 502 unigenes as compared to the yellow-seeded one. Biological pathway analysis revealed the involvement of forty six unigenes in flavonoid biosynthesis. The unigenes encoding dihydroflavonol reductase (DFR), leucoantho-cyanidin dioxygenase (LDOX) and anthocyanidin reductase (ANR) for late flavonoid biosynthesis were not expressed at all or at a very low level in the yellow-seeded testa, which implied that these genes for PAs biosynthesis be associated with seed color of B. juncea, as confirmed by qRT-PCR analysis of these genes. To our knowledge, it is the first time to sequence the transcriptome of seed coat in Brassica juncea. The unigene sequences obtained in this study will not only lay the foundations for insight into the molecular mechanisms underlying seed pigmentation in B. juncea, but also provide the basis for further genomics research on this species or its allies.
语种：
英文
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Genome comparison of two Magnaporthe oryzae field isolates reveals genome variations and potential virulence effectors
作者：
Chen, Chenxi;Lian, Bi;Hu, Jinnan;Zhai, Huanchen;Wang, Xingxing;...
期刊：
BMC Genomics ,2013年14(1):1-12 ISSN：1471-2164
通讯作者：
Wang, Zonghua
作者机构：
[Chen, Chenxi; Venu, R. C.; Wang, Guo-Liang; Hu, Jinnan; Lian, Bi; Mitchell, Thomas K.] Ohio State Univ, Dept Plant Pathol, Columbus, OH 43210 USA.;[Chen, Meilian; Wang, Zonghua; Wang, Xingxing; Lian, Bi; Zhai, Huanchen; Wang, Baohua] Fujian Agr & Forestry Univ, Minist Educ, Key Lab Biopesticide & Chem Biol, Fuzhou 350002, Fujian, Peoples R China.;[Wang, Guo-Liang; Liu, Erming; Wang, Zhilong] Hunan Agr Univ, Coll Agron, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China.;[Zhai, Huanchen] Henan Univ Technol, Coll Bioengn, Zhengzhou 450001, Henan, Peoples R China.;[Wang, Zonghua] Fujian Agr & Forestry Univ, Minist Educ, Key Lab Biopesticide & Chem Biol, 15 Shangxiadian Rd, Fuzhou 350002, Fujian, Peoples R China.
通讯机构：
[Wang, Zonghua] F;Fujian Agr & Forestry Univ, Minist Educ, Key Lab Biopesticide & Chem Biol, 15 Shangxiadian Rd, Fuzhou 350002, Fujian, Peoples R China.
关键词：
Magnaporthe oryzae;Next generation sequencing;Genome comparison;Candidate effectors identification;Isolate-specific genome content
摘要：
Rice blast caused by the fungus Magnaporthe oryzae is an important disease in virtually every rice growing region of the world, which leads to significant annual decreases of grain quality and yield. To prevent disease, resistance genes in rice have been cloned and introduced into susceptible cultivars. However, introduced resistance can often be broken within few years of release, often due to mutation of cognate avirulence genes in fungal field populations. To better understand the pattern of mutation of M. oryzae field isolates under natural selection forces, we used a next generation sequencing approach to analyze the genomes of two field isolates FJ81278 and HN19311, as well as the transcriptome of FJ81278. By comparing the de novo genome assemblies of the two isolates against the finished reference strain 70–15, we identified extensive polymorphisms including unique genes, SNPs (single nucleotide polymorphism) and indels, structural variations, copy number variations, and loci under strong positive selection. The 1.75 MB of isolate-specific genome content carrying 118 novel genes from FJ81278, and 0.83 MB from HN19311 were also identified. By analyzing secreted proteins carrying polymorphisms, in total 256 candidate virulence effectors were found and 6 were chosen for functional characterization. We provide results from genome comparison analysis showing extensive genome variation, and generated a list of M. oryzae candidate virulence effectors for functional characterization.
语种：
英文
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Allocation and dynamics of assimilated carbon in rice-soil system depending on water management
作者：
Tian, Jing;Pausch, Johanna;Fan, Mingsheng* ;Li, Xiaolin;Tang, Qiyuan;...
期刊：
Plant and Soil ,2013年363(1-2):273-285 ISSN：0032-079X
通讯作者：
Fan, Mingsheng
作者机构：
[Li, Xiaolin; Tian, Jing; Fan, Mingsheng] China Agr Univ, Minist Educ, Key Lab Plant Soil Interact, Beijing 100193, Peoples R China.;[Li, Xiaolin; Tian, Jing; Fan, Mingsheng] China Agr Univ, Coll Resources & Environm Sci, Beijing 100193, Peoples R China.;[Pausch, Johanna] Univ Bayreuth, Dept Agroecosyst Res, D-95440 Bayreuth, Germany.;[Kuzyakov, Yakov; Tian, Jing; Pausch, Johanna] Univ Gottingen, Dept Soil Sci Temperate Ecosyst, D-37077 Gottingen, Germany.;[Tang, Qiyuan] Hunan Agr Univ, Coll Agr, Changsha 410128, Peoples R China.
通讯机构：
[Fan, Mingsheng] C;China Agr Univ, Minist Educ, Key Lab Plant Soil Interact, Beijing 100193, Peoples R China.
关键词：
Water conservation techniques;Paddy soil;Isotopic labeling;Carbon allocation;Roots exudation;Rhizodeposition
摘要：
Aims: Although water conservation in rice production has become increasingly important, the effects of water management on the allocation and dynamics of carbon (C) within the rice-soil system remain unknown. Methods: We compared the allocation and dynamics of C assimilated by rice under continuously flooded, non-flooded and alternate water regimes. Rice (Oryza sative L. cv. Luliangyou 996) was labeled with 14CO2 and harvested 7 times within 45 days. Results: More 14C was released from roots into the soil in non-flooded and alternate water regimes treatments. Microbial 14C decreased with time after the labeling and was lowest under flooded condition. Roots and rhizomicrobial respiration followed the order of non-flooded > alternate water regimes > flooded treatment. Water management affected 14C distribution in aggregates with more 14C in macroaggregates in the non-flooded treatment. Estimated amounts of C transferred remaining belowground by rice 45 days after labeling were 1,986, 2,827 and 2,472 kg C ha-1, of which rhizodeposition accounted for about 41 %, 16 % and 30 % of C transferred belowground under non-flooded, flooded and alternate water regimes, respectively. Conclusions: Water management affected the allocation and dynamics of recently assimilated C within the rice-soil system and also changed the relative contribution of rhizodeposition to C transferred belowground. This study suggests the differences in the driving mechanisms of C sequestration under flooded vs. non-flooded and alternate water regimes. © 2012 Springer Science+Business Media B.V.
语种：
英文
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An allotetraploid Brassica napus early-floweringmutant has BnaFLC2-regulated flowering
作者：
Huang, Yong;Jiang, Ling;Ruan, Ying;Shen, Wenhui;Liu, Chunlin*
期刊：
Journal of the Science of Food and Agriculture ,2013年93(15):3763-3768 ISSN：0022-5142
通讯作者：
Liu, Chunlin
作者机构：
[Ruan, Ying; Huang, Yong; Liu, Chunlin; Shen, Wenhui; Jiang, Ling] Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China.;[Ruan, Ying; Huang, Yong; Shen, Wenhui; Jiang, Ling] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Liu, Chunlin] Hunan Agr Univ, Coll Agr, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liu, Chunlin] H;Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China.
关键词：
Brassica napus;early-flowering mutant;cytological observations;BnaFLC2
摘要：
BACKGROUND: Flowering time is an important agronomic trait, and wide variation in flowering time exists among Brassica napus accessions. GX50 early-flowering mutant, induced from Brassica napus by Ethyl Methane Sulfonate (EMS), exhibits a remarkable early transition from vegetative to reproductive growth. RESULTS: GX50 plants flowered about 60 days earlier than the control wild-type plant B. napus XY15 under greenhouse conditions. Cytological examination revealed that the GX50 plants form inflorescences as early as from 5 weeks old, flower primordium from 6 weeks old, and siliques from 10 weeks old, whereas 10-week-old XY15 plants are still at vegetative growth stage. To unravel the molecular mechanisms underlying the GX50 flowering phenotype, we analyzed the expression of several key regulatory genes. Expressions of all five BnaFLCs (BnaFLC1 to BnaFLC5), BnaFT and BnaSOC1 were detected. Interestingly, BnaFLCs expression levels were lower in GX50 than those in XY15. Among the five BnaFLCs, only the expression pattern of BnaFLC2 corresponded to the timing of floral organ differentiation in GX50. In agreement with previous knowledge that BnaFLCs repress expression of BnaFT and BnaSOC1, increased levels of BnaFT and BnaSOC1 were observed in GX50 compared with XY15. CONCLUSION: BnaFLC2, but not the other BnaFLC genes, plays an important role in B. napus GX50 floral transition. © 2013 Society of Chemical Industry.
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Using proteomic analysis to find the proteins involved in resistance against Sclerotinia sclerotiorum in adult Brassica napus
作者：
Wen, Li* ;Tan, Tai-Long;Shu, Jia-Bin;Chen, Ying;Liu, Ying;...
期刊：
European Journal of Plant Pathology ,2013年137(3):505-523 ISSN：0929-1873
通讯作者：
Wen, Li
作者机构：
[Tan, Tai-Long; Shu, Jia-Bin; Guan, Chun-Yun; Wen, Li; Yin, Ming-Zhi; Zhang, Qiu-Ping] Hunan Agr Univ, Oilseed Crop Res Inst, Natl Oilseed Crop Improvement Ctr, Changsha 410128, Hunan, Peoples R China.;[Liu, Ying; Yang, Zhong-Fang; Chen, Ying; Tao, Jun; Wen, Li] Changsha Univ Sci & Technol, Dept Food & Biol Engn, Changsha 410114, Hunan, Peoples R China.
通讯机构：
[Wen, Li] H;Hunan Agr Univ, Oilseed Crop Res Inst, Natl Oilseed Crop Improvement Ctr, Changsha 410128, Hunan, Peoples R China.
关键词：
Brassica napus;Sclerotinia sclerotiorum;Proteome;Antioxidation
摘要：
Sclerotinia disease, caused by Sclerotinia sclerotiorum, is one of the most serious plant diseases in China. Research on the mechanism of disease resistance to S. sclerotiorum will help solve control problems. In this study, near-isogenic lines were first used in combination with the proteomic technique. A comparison of protein expression profiles in a susceptible line with those in a resistant line during the interaction of adult Brassica napus with S. sclerotiorum resulted in the identification of 20 important proteins related to disease resistance. Those proteins were then determined to be involved in various functions, including pathogen resistance, antioxidation, and transcription regulation. Our finding showed that some proteins involved in defence-a glycine rich protein (GRP); a trypsin inhibitor protein (TIP); two heat shock proteins (HSPs); and a thiol methyltransferase (TMT)-were upregulated or expressed specially in the resistant B. napus lines. These proteins contribute to ROS (reactive oxygen species) elimination and pathogen-defence in the resistant line, which would help the host defend itself against S. sclerotiorum. As a consequence, the onset of PCD (programmed cell death) was delayed, and the spread of S. sclerotiorum was slowed in the resistant line. Presented results underline the role of specific proteins in the disease process. By building on these results, future research may help determine the genes that are important in conveying resistance to S. sclerotiorum in B. napus. © 2013 KNPV.
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英文
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Identification of Potato mop-top virus (PMTV) in potatoes in China
作者：
Hu, Xinxi* ;Lei, Yan;Xiong, Xingyao;He, Changzheng;Liu, Mingyue;...
期刊：
Canadian Journal of Plant Pathology ,2013年35(3):402-406 ISSN：0706-0661
通讯作者：
Hu, Xinxi
作者机构：
[Hu, Xinxi; Lei, Yan; Liu, Mingyue; Xiong, Xingyao; He, Changzheng] Hunan Agr Univ, Coll Hort & Landscape, Hunan Prov Engn Res Ctr Potatoes, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China.;[Nie, Xianzhou] Agr & Agri Food Canada, Potato Res Ctr, Fredericton, NB E3B 4Z7, Canada.
通讯机构：
[Hu, Xinxi] H;Hunan Agr Univ, Coll Hort & Landscape, Hunan Prov Engn Res Ctr Potatoes, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China.
关键词：
Potato;Potato mop-top virus;spraing disease;molecular detection
摘要：
Potato tubers exhibiting necrotic rings/arcs were found in a winter potato crop 'Favorita' in the subtropical area Huidong county, Guangdong province, China, in 2012. When the symptomatic tubers were cut crosswise, light to dark brown necrotic arcs were observed in the tuber flesh. A mini-survey of the crop found 8% symptomatic tubers in an area of 10 meters2. To reveal the causal agent of the disease, reverse transcription-polymerase chain reactions (RT-PCR) targeting 9 common potato viruses including those viruses (e.g, Potato mop-top virus (PMTV), Tobacco rattle virus, Tomato spotted wilt virus, and Potato virus Y tuber necrosis strain) known to be capable of induction of tuber necrosis, were carried out. Except for PMTV, no other viruses were detected. Sequencing of the 460 bp amplicon revealed that the fragment exhibited a 99-100% sequence identify with most PMTV coat protein sequences deposited in GenBank, which was confirmed by a further phylogenetic analysis. Enzyme linked immunosorbent assay with PMTV antibody on the symptomatic tubers was also carried out, and a positive reading was observed. Together, these results demonstrate that PMTV was the causal agent for the spraing disease in potatoes in the winter crop in Guangdong. To our knowledge, this is the first report of PMTV in Guangdong province, China, and the first scientific confirmation of PMTV in China. © 2013 The contribution of Xianzhou Nie.
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英文
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Antimicrobial activity of various extracts from different parts of amaranthus mangostanus
作者：
Zhang, Ya;Su, Pin;Huang, Huang;Liu, Shuangqing;Liao, Xiaolan*
期刊：
Asian Journal of Chemistry ,2013年25(11):6311-6315 ISSN：0970-7077
通讯作者：
Liao, Xiaolan
作者机构：
[Zhang, Ya; Liu, Shuangqing; Su, Pin; Liao, Xiaolan] Hunan Agr Univ, Coll Biosafety Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Huang, Huang] Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liao, Xiaolan] H;Hunan Agr Univ, Coll Biosafety Sci & Technol, Changsha 410128, Hunan, Peoples R China.
关键词：
Amaranthus mangostanus;Antimicrobial activity;Extracts
摘要：
Amaranthus mangostanus is a green vegetable which is also used as sudorific, febrifuge, emollient, lactogogue and a specific treatment for colic. This study was aimed to determine antibacterial and antifungal activities of the methanol, dichloromethane, petroleum ether and ethyl acetate extracts from different parts of A. mangostanus against microbes that cause plant or human diseases and food contamination. At concentrations ranging from 40-100 mg/mL, ethyl acetate extracts from leaves and stems exhibited concentration-dependent inhibition against Pseudomonas solanacearum Smith, Acidovorax avenae subsp. A. Citrulli, Rhizoctonia solani, Colletotrichum capsici (syd.) Butl, Pseudomonas aeruginosa, Bacillus cereus and Escherichia coli, but not Magnaporthe grisea, Alternaria alternata Keissler, Phytophtora parasitica var. nicotianae Tucker and Fusarium graminearum Sehw., with MIC values of 519-713 and 463-697 mg/mL, respectively, whereas the ethyl acetate extract from roots only showed antibacterial activity against P. solanacearum Smith with an MIC of 815 mg/mL. Ethyl acetate extracts from leaves and stems also showed inhibitory effect on the fungus R. solani, with MICs of 686 and 734 mg/mL, respectively. Consequently the information on the antimicrobial functions of the extracts can shed light on the discovery of natural products for management of plant and human infectious diseases.
语种：
英文
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Three lignocellulose features that distinctively affect biomass enzymatic digestibility under NaOH and H2SO4 pretreatments in Miscanthus
作者：
Zhang, Wei;Yi, Zili;Huang, Jiangfeng;Li, Fengcheng;Hao, Bo;...
期刊：
Bioresource Technology ,2013年130:30-37 ISSN：0960-8524
通讯作者：
Peng, Liangcai
作者机构：
[Hao, Bo; Li, Ming; Peng, Liangcai; Zhang, Wei; Hong, Shufen; Sun, Wei; Li, Fengcheng; Huang, Jiangfeng] Natl Key Lab Crop Genet Improvement, Wuhan, Peoples R China.;[Hao, Bo; Li, Ming; Peng, Liangcai; Zhang, Wei; Hong, Shufen; Sun, Wei; Li, Fengcheng; Huang, Jiangfeng] Natl Ctr Plant Gene Res, Wuhan, Peoples R China.;[Hao, Bo; Li, Ming; Peng, Liangcai; Lv, Yezi; Zhang, Wei; Hong, Shufen; Sun, Wei; Li, Fengcheng; Huang, Jiangfeng] Hunan Agr Univ, Biomass & Bioenergy Res Ctr, Changsha 410128, Hunan, Peoples R China.;[Peng, Liangcai; Hong, Shufen; Sun, Wei; Li, Fengcheng; Huang, Jiangfeng] Hunan Agr Univ, Coll Plant Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Hao, Bo; Li, Ming; Peng, Liangcai; Zhang, Wei] Hunan Agr Univ, Coll Life Sci & Technol, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Peng, Liangcai] N;Natl Key Lab Crop Genet Improvement, Wuhan, Peoples R China.
关键词：
Biomass enzymatic digestibility;Crystallinity index (CrI);Degree of polymerization (DP);Miscanthus;Mole number (MN)
摘要：
In this study, total 80 typical Miscanthus accessions were examined with diverse lignocellulose features, including cellulose crystallinity (CrI), degree of polymerization (DP), and mole number (MN). Correlation analysis revealed that the crude cellulose CrI and MN, as well as crystalline cellulose DP, displayed significantly negative influence on biomass enzymatic digestibility under pretreatments with NaOH or H2SO4 at three concentrations. By contrast, the comparative analysis of two Miscanthus samples with similar cellulose contents showed that crude cellulose DP and crystalline cellulose MN were positive factors on biomass saccharification, indicating cross effects among the cellulose levels and the three cellulose features. The results can provide insights into mechanism of the lignocellulose enzymatic digestion, and also suggest potential approaches for genetic engineering of bioenergy crops. © 2012 Elsevier Ltd.
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英文
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Comparisons of Yield and Growth Behaviors of Hybrid Rice Under Different Nitrogen Management Methods in Tropical and Subtropical Environments
作者：
Md, Ibrahim;Peng Shao-bing;Tang Qi-yuan;Huang Min;Jiang Peng;...
期刊：
农业科学学报(英文) ,2013年12(4):621-629 ISSN：2095-3119
通讯作者：
Zou Ying-bin
作者机构：
[Md, Ibrahim; Tang Qi-yuan; Jiang Peng; Huang Min; Zou Ying-bin] Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.;[Peng Shao-bing] Huazhong Agr Univ, Crop Physiol & Prod Ctr, Wuhan 430070, Peoples R China.
通讯机构：
[Zou Ying-bin] H;Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.
关键词：
crop growth rate;biomass production;hybrid rice;leaf area duration;leaf area index;real-time nitrogen management
摘要：
To compare the grain yield and growth behaviors of hybrid rice, field experiments were conducted in a subtropical environment in Changsha, Hunan Province, China, and in two tropical environments in Gazipur and Habiganj in Bangladesh during 2009 to 2011. Three hybrid rice cultivars were grown under three nitrogen (N) management treatments in each experiment. The results showed that grain yield was significantly affected by locations, N treatments and their interaction but not by cultivars. Changsha produced 8-58% higher grain yields than Bangladesh locations. Sink size (spikelet number per unit land area) was responsible for these yield differences. Larger panicle size (spikelet number per panicle) contributed to greater sink size in Changsha. Aboveground total biomass was greater in Changsha than in Bangladesh locations, whereas harvest index was higher in Bangladesh locations than in Changsha. Crop growth rate (CGR) was greater at Changsha than Bangladesh locations during vegetative phase, while the difference was relatively small and not consistent during the later growth phases. Higher leaf area index and leaf area duration were partly responsible for the greater CGR in Changsha. Real-time N management (RTNM) produced lower grain yields than fixed-time N management in more than half of the experiments. Our study suggested that further improvement in rice yield in the tropical environments similar to those of Bangladesh will depend mainly on the ability to increase panicle size as well as CGR during vegetative phase, and the chlorophyll meter threshold value used in RTNM needs to be modified according to environmental conditions and cultivar characteristics to achieve a desirable grain yield.
语种：
英文
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Maize LAZY1 Mediates Shoot Gravitropism and Inflorescence Development through Regulating Auxin Transport, Auxin Signaling, and Light Response
作者：
Dong, Zhaobin;Jiang, Chuan;Chen, Xiaoyang;Zhang, Tao;Ding, Lian;...
期刊：
PLANT PHYSIOLOGY ,2013年163(3):1306-1322 ISSN：0032-0889
通讯作者：
Jin, Weiwei
作者机构：
[Dong, Zhaobin; Jin, Weiwei; Chen, Xiaoyang; Lai, Jinsheng; Song, Weibin] China Agr Univ, Coordinated Res Ctr Crop Biol, Beijing Key Lab Crop Genet Improvement, Natl Maize Improvement Ctr China, Beijing 100193, Peoples R China.;[Jiang, Chuan] Shandong Agr Univ, Coll Agr, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China.;[Zhang, Tao] Univ Elect Sci & Technol China, Sch Life Sci & Technol, Chengdu 610054, Sichuan, Peoples R China.;[Zhang, Xiaolan; Ding, Lian] China Agr Univ, Dept Vegetable Sci, Beijing Key Lab Growth & Dev Regulat Protected Ve, Beijing 100193, Peoples R China.;[Luo, Hongbing] Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Jin, Weiwei] C;China Agr Univ, Coordinated Res Ctr Crop Biol, Beijing Key Lab Crop Genet Improvement, Natl Maize Improvement Ctr China, Beijing 100193, Peoples R China.
摘要：
Auxin is a plant hormone that plays key roles in both shoot gravitropism and inflorescence development. However, these two processes appear to be parallel and to be regulated by distinct players. Here, we report that the maize (Zea mays) prostrate stem1 mutant, which is allelic to the classic mutant lazy plant1 (la1), displays prostrate growth with reduced shoot gravitropism and defective inflorescence development. Map-based cloning identified maize ZmLA1 as the functional ortholog of LAZY1 in rice (Oryza sativa) and Arabidopsis (Arabidopsis thaliana). It has a unique role in inflorescence development and displays enriched expression in reproductive organs such as tassels and ears. Transcription of ZmLA1 responds to auxin and is repressed by light. Furthermore, ZmLA1 physically interacts with a putative auxin transport regulator in the plasma membrane and a putative auxin signaling protein in the nucleus. RNA-SEQ data showed that dozens of auxin transport, auxin response, and light signaling genes were differentially expressed in la1 mutant stems. Therefore, ZmLA1 might mediate the cross talk between shoot gravitropism and inflorescence development by regulating auxin transport, auxin signaling, and probably light response in maize.
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英文
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Mechanism and capacities of reducing ecological cost through rice-duck cultivation
作者：
Long, Pan;Huang, Huang* ;Liao, Xiaolan;Fu, Zhiqiang;Zheng, Huabin;...
期刊：
Journal of the Science of Food and Agriculture ,2013年93(12):2881-2891 ISSN：0022-5142
通讯作者：
Huang, Huang
作者机构：
[Long, Pan; Fu, Zhiqiang; Zheng, Huabin; Huang, Huang; Chen, Can] Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.;[Long, Pan] China Agr Univ, Coll Agron & Biotech, Beijing 100193, Peoples R China.;[Fu, Zhiqiang; Zheng, Huabin; Huang, Huang; Chen, Can; Liao, Xiaolan] Hunan Agr Univ, Minist Agr, Key Lab Multicropping Cultivat & Farming Syst, Changsha 410128, Hunan, Peoples R China.;[Liao, Xiaolan] Hunan Agr Univ, Coll Biosafety Sci & Technol, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Huang, Huang] H;Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.
关键词：
ecological cost;economic benefit;reduce;rice-duck cultivation
摘要：
Background Rice-duck cultivation is the essence of Chinese traditional agriculture. A scientific assessment of the mechanism and its capacity is of theoretical significance and practical value in improving modern agricultural technology. Results The duck's secretions, excreta and their treading, pecking and predation decrease the occurrence of plant diseases, pests and weeds, enrich species diversity and improve the field environment. The rice-duck intergrowth system effectively prevents rice planthoppers and rice leafhoppers. The control effects can be up to 98.47% and 100% respectively; it also has effects on the control of Chilo suppressalis, Tryporyza incertulas and the rice leafrollers. Notable control results are found on sheath blight, while the effects on other diseases are about 50%. Harm from weeds is placed under primary control; prevention of weeds is sequenced by broadleaf weeds>sedge weeds>Gramineae weeds. Contents of soil organic matter, N, P and K are improved by the system; nutrient utilization is accelerated, resulting in decreased fertilizer application. Greenhouse gas emissions are reduced by 1-2% and duck fodder is saved in this system. There is also an obvious economic benefit. Conclusion Compared to conventional rice cultivation, rice-duck cultivation shows great benefits to ecologic cost and economic income. (c) 2013 The Authors. Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry
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英文
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Phylogenetic Analysis of Brassica rapa MATH-Domain Proteins
作者：
Zhao, Liming;Huang, Yong;Hu, Yan;He, Xiaoli;Shen, Wenhui;...
期刊：
CURRENT GENOMICS ,2013年14(3):214-223 ISSN：1389-2029
通讯作者：
Liu, Chunlin
作者机构：
[Zhao, Liming; Ruan, Ying; Huang, Yong; Hu, Yan; Liu, Chunlin; Shen, Wenhui] Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China.;[Zhao, Liming; Ruan, Ying; Huang, Yong; Hu, Yan; Shen, Wenhui] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[He, Xiaoli] Hunan Agr Univ, Expt Ctr, Changsha 410128, Hunan, Peoples R China.;[Liu, Chunlin] Hunan Agr Univ, Coll Agr, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liu, Chunlin] H;Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China.
关键词：
Brassica rapa;Phylogenetic analysis;MATH domain protein;Protein domain organization;Gene expression;Seed development.
摘要：
The MATH (meprin and TRAF-C homology) domain is a fold of seven anti-parallel β-helices involved in protein- protein interaction. Here, we report the identification and characterization of 90 MATH-domain proteins from the Brassica rapa genome. By sequence analysis together with MATH-domain proteins from other species, the B. rapa MATH-domain proteins can be grouped into 6 classes. Class-I protein has one or several MATH domains without any other recognizable domain; Class-II protein contains a MATH domain together with a conserved BTB (Broad Complex, Tramtrack, and Bric-a-Brac) domain; Class-III protein belongs to the MATH/Filament domain family; Class-IV protein contains a MATH domain frequently combined with some other domains; Class-V protein has a relative long sequence but contains only one MATH domain; Class-VI protein is characterized by the presence of Peptidase and UBQ (Ubiquitinylation) domains together with one MATH domain. As part of our study regarding seed development of B. rapa, six genes are screened by SSH (Suppression Subtractive Hybridization) and their expression levels are analyzed in combination with seed developmental stages, and expression patterns suggested that Bra001786, Bra03578 and Bra036572 may be seed development specific genes, while Bra001787, Bra020541 and Bra040904 may be involved in seed and flower organ development. This study provides the first characterization of the MATH domain proteins in B. rapa. ©2013 Bentham Science Publishers.
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