摘要：
In order to discover the formation mechanism of carotenoid derived aroma, which has been wildly used on protection of crop against insect attacks, the full-length cDNA of an Artemisia annua carotenoid cleavage dioxygenase (AaCCD1) was cloned by rapid amplification of cDNA ends. The function of AaCCD1 was characterized by expression of AaCCD1 in a strain of E. coli accumulating carotenoids and enzyme assay in vitro. The completed open read frame of AaCCD1 was 1629 bp and it encoded a 542-amino acid protein with a 77% amino acid identity to Arabidopsis thaliana CCD1, a predicted molecular mass of 61.04 kDa and a pI of 5.8. AaCCD1 efficiently cleaves carotenoids and regulate the formation of terpenoid compounds. This is the first time to report the cloning and identification of carotenoid cleavage dioxygenase from Atemisia annua, which will play a great role on understanding the regulation of volatile compounds.
摘要：
The studies compares peroxidase activity and the enzyme joint activity of Glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase in different position at germination of diploid and triploid watermelon seed. The results show that enzyme activities in different part of diploid watermelon seed are higher than in triploid watermelon seed, and enzyme activities of the seed coat is the lowest, enzyme activities of the endopleura is the highest in the three part of the triploid watermelon seeds. The peak enzyme activity of each part of the triploid seeds is later than the diploid. The enzyme joint activity of Glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase had obvious change in 24h and 36h after germination respectively; the enzyme joint activity of Glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase rose quikly in 0h-12h germination in the diploid's embryo, and peaked at 0.49 mu.g(-1).min(-1) at 24h germination and began to reduced until it stabilized at about 0.2 mu.g(-1).min(-1) at 36h germination. The enzyme joint activity of Glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase rise very slowly in 0h-36h germination in the triploid's embryo, it stabilized at about 0.18 mu.g(-1).min(-1) at 36h germination.
会议名称：
2nd International Symposium on Citrus Biotechnology
会议时间：
NOV 30-DEC 02, 2009
会议地点：
Catania, ITALY
会议主办单位：
[Qin, L.;Luo, S. L.;Deng, Z. N.;Yan, J. W.;Li, N.;Yuan, F. R.] Hunan Agr Univ, Natl Ctr Citrus Improvement, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China.
会议论文集名称：
Acta Horticulturae
关键词：
'Succari' sweet orange;Resistance to canker disease;Terf1 transformation;Tolerance to abiotic stresses
摘要：
The transcription factors TERF1 gene was transferred into 'Succari' sweet orange [Citrus sinensis (L.) Osbeck] via Agrobacterium infection and 7 transgenic clones were obtained. Three transgenic clones were further tested for tolerance to water deficiency, low temperature and their resistance to citrus canker disease (Xanthomonas axonopodis pv. citri). Under 10% of soil relative water content (SRWC), transgenic clones showed stronger tolerance than the wild type, with normal growing, leas leaf chlorophyll content decrease, higher Proline (Pro) accumulation and activity of the protective enzymes (CAT, POD and SOD). Simultaneously, transgenic clones showed stronger tolerance to low temperature stress. When plants being treated at -10 degrees C for 30 min, all the wild type plants completely died within 5 days, while the transgenic plants merely revealed severe damages on the shoots and leaves, the main stem and the roots were still alive. The terf1 transgenic clones displayed resistance to citrus canker disease. In the in vitro assay and vivo assay, transgenic clones obviously decreased the disease incidence rate. When the transgenic clones were inoculated with 104 and 105 cfu/ml of citrus bacterial canker pathogen, no symptom occurred. The results indicated that the tested transgenic clones showed obvious tolerance to abiotic stresses and resistance to canker disease. Further investigation in field is going on.
会议名称：
28th International Horticultural Congress on Science and Horticulture for People (IHC) / IHC Seminar on New Look at Medicinal and Aromatic Plants
摘要：
Poria cocos (Schw.) Wolf is a well-known traditional Chinese herbal medicine that has been used as diuretic, sedative and tonic drug. However, the natural P. cocos forms sclerotia on the roots of cedar or pine, its productivity is limited. Therefore, it is imperative to find a cultivated P. cocos substitute for the natural one. A new cultivated P. cocos strain numbered 28 was selected in the State Key Laboratory of Sub-health Intervention Technology. In this study, antagonistic effect was done to test the relationship between P. cocos 28 and a natural strain (numbered 578). The molecular marker SRAP (Sequences-related Amplified Polymorphism) amplification and cloning, sequencing of 2 ORFs (Open Reading Frame) fragments were applied to analyze their genetic diversity. The phenol-sulfuric acid procedure, spectrophotometry and amino acid standard analysis methods were used to determine the concentration of active ingredients - polysaccharide, triterpene and amino acid, respectively. There was an obscurely shaped line in the antagonistic effect and none of 132 SRAP primer combinations turned out to be polymorphic. Sequencing of 2 ORFs fragments showed that there were only 59 mutant sites with 25 conversion, 22 transversion and 12 insertion or deletion. No significant difference was detected in the contents of active ingredients between P. cocos 28 and 578. Therefore, the cultivated P. cocos 28 could be the alternative strain of natural P. cocos 578. This study indicated that antagonistic effect and SRAP marker could be effective methods to analyze the relationship of P. cocos and would be useful in selection of high-yield cultivation of P. cocos.
会议主办单位：
[Sun, Xiaowu] Hunan Agr Univ, Coll Landscape Architecture & Hort, Changsha 410128, Hunan, Peoples R China.^[Wang, Yongfei;Ma, Sanmei;Wang, Lixian;Yu, Mengjie] Jinan Univ, Dept Biotechnol, Guangzhou 510632, Guangdong, Peoples R China.^[Wang, Lixian;He, Xiaoming] Guangdong Acad Agr Sci, Vegetable Res Inst, Guangzhou 510640, Peoples R China.^[Sun, Xiaowu] Hunan Res Inst Melons, Shaoyang 422001, Peoples R China.
会议论文集名称：
Acta Horticulturae
关键词：
Chieh-qua (Benincasa hispida Cogn. var. chieh-qua How.);Fusarium wilt;Resistant clone
摘要：
The leaves of regenerated Chieh-qua (Benincasa hispida Cogn. var. chieh-qua How.) plants from the fusaric acid (FA) resistance and other varieties with different resistance to fusarium wilt were examined to analyze the activity of peroxidase (POD), polyphenoloxidase (PPO), and the content of soluble protein when infected with Fusarium oxysporum. The activity of POD and PPO was greater in F. oxysporum treated plants compared to control. The content of soluble protein in three varieties was lower than that of the control, and significant difference was observed. It suggested that resistant mechanism to fusarium wilt in resistant clone selected in vitro were similar with that in resistant variety obtained by traditional method.
会议名称：
2009 Conference on the Key Technology of Horticultural Produce(2009园艺生产关键技术学术会议 KTHP 2009)
会议时间：
2009-04-11
会议地点：
北京
会议论文集名称：
2009 Conference on the Key Technology of Horticultural Produce(2009园艺生产关键技术学术会议 KTHP 2009)论文集
关键词：
Golden section;Plant hormones;Drainageline;Water-saving cultivation
摘要：
The correlation between biomass and drainage line was behaved by the effect of water on root growth. The result showed that a ratio of aeration part over the drainage line up to 0.6 kept more than 60 percent of roots being in a good aeration condition for a long duration, resulted in the increasing of the CTK and IAA concentration and decreasing of the GA and ABA concentration in roots, and promoted the emergence of new roots and branches. Regression analysis of biomass showed that there was a production peak when the drainage line was near to the golden section. The theory of The Golden Drainage Cultivating Method and its technique were summarized through the experiments on kinds of plants for several years, and its key factor was that the drainage line for plant should be determined at the golden section of roots from top to bottom.
摘要：
Water stress in loquat (Eriobotrya japonica Lindl.) reduced both total and shoot dry matter (DW), but increased root DW, compared to the control. Plant height and stem diameter were reduced by 50 and 46%, respectively. Leaf and shoot RWC decreased significantly to 75.1 and 73.6% under water stress (20% full water holding capacity of soil. Water stress significantly increased concentrations of NH3- NH4+, arginine and proline, resulting in negative correlations between leaf RWC and concentrations of NH3-NH4+, arginine and proline in leaves. Under 20% PC treatment, Pn was only 3.98 μmol/m2.s, which was 63.08% lower than the control.
摘要：
The value of fluorescent amplified fragment length polymorphism (FAFLP) fingerprinting for identifying genotypes and analyzing genetic variation in genus Actinidia was assessed. Characterization of FAFLP markers and their use in fingerprinting 31 kiwifruit genotypes grown in Jiangxi Province, China is reported. Of the eight primer pairs assessed, four (E-AAC + M-CAC, E-AAG + M-CTG, E-AAC + M-CAG and E-AAC + M-CTA) were screened because of the number and quality of polymorphic fragments they produced. A total of 190 FAFLP markers were scored using the four primer pairs. 179 (94.2%) were polymorphic and showed a high level of genetic diversity. All kiwifruit genotypes tested could be uniquely identified using the four primer pairs.
关键词：
Fruit storage;Putrescine;Reticulata;Salicylic acid;Spermidine;Spermine
摘要：
Seven-year-old 'Ponkan' mandarin (Citrus reticulate Blanco) on trifoliate rootstocks (Poncirus triforliata L.) were employed monitor as to the changes of endogenous polyamines (PAs) and salicylic acid (SA) concentrations pre- and postharvest, and correlated to postharvest storage life. The results showed that endogenous concentrations of free polymines and SA peaked in October at a maximum of 1,233 nmol·g-1FW and 2,150 ng·g -1FW, respectively. Then declined gradually during postharvest storage. The decline paralleled observed peel senescence. Fruits harvest in December were dipped into 100 mg·L-1 putrescine (Put), spermidine (Spd), spermine (Spm), and 400 mg·L-1 SA, with distilled water as the control and stored for 3 months. Dipping elevated endogenous concentrations Pas and Sa, and the postharvest storage life was prolonged. Except Put, all treatments resulted in the higher concentrations of endogenous Pas and SA in treated fruits compared to the control. During 3 months storage, SA had 2.0% decay, 3.5% for Spm, 4.0% for Spd, and 6.4% for Put treatment. Weight loss for the treated fruit was 3.0% Spm, 3.5% for SA and Spd, and 5.3% for Put treatment. Control fruit showed 13.5% decay and 11.0% weight loss during 3 months period of storage. The application of PA and SA increased endogenous polymine (PA) and SA concentrations, which led to an improvement in fruit quality and prolonged storage life. SA, Spm, and Spd treatments were better than a Put treatment.
