作者机构：
College of Bio-Safety Science and Technology,Hunan Agricultural University,Changsha 410128,China
会议名称：
第十一届全国农药学科教学科研研讨会
会议时间：
201007
会议地点：
长沙
会议论文集名称：
第十一届全国农药学科教学科研研讨会论文集
关键词：
Derris cavaleriei vines;Plutella xylostella;antifeedant compounds;isolonchcarpin;magnificol;millettocalyxin C
摘要：
In order to determine the active compounds from Denis cavaleriei vines and their antifeedant activities against Plutella xylostella larvae,the active compounds were isolated by activity-guided fractionation with column chromatography and identified on the basis of MS and NMR data,and antifeedant activity was determined by sandwich method.The results showed that four compounds,isolonchcarpin (A),Magnificol (B),ovalifla,vanone (C) and millettocaiyxin C (D) had been isolated from the plant vines for the first time.Isolonchcarpin,Magnificol and millettocalyxin C had been exhibited antifeeding activity,with AFC50 values of 25.5 mg·L-1,92.8 mg·L-1 and 115.9 mg·L-1 against the third instar larvae of P.xylostella at 48 h after treatment,respectively.The above-mentioned three compounds were main antifeedant compounds derived from the plant vines against P.xylostella.The elucidation of these chemicals is important not only for understanding the insect-plant relationships,but also for their potential in P.xylostella control.
作者：
Zhong, Jie;Lei, Xiang Hua;Zhu, Jun Zi;Song, Ge;Zhang, Ya Dong;...
期刊：
Archives of Virology,2014年159(11):3063-3070 ISSN：0304-8608
通讯作者：
Gao, Bi Da
作者机构：
[Chen, Yi; Gao, Bi Da; Zhong, Jie; Lei, Xiang Hua; Song, Ge; Zhang, Ya Dong; Zhu, Jun Zi] Hunan Agr Univ, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha 410128, Hunan, Peoples R China.;[Gao, Bi Da] Hunan Agr Univ, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Nongda Rd 1, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Gao, Bi Da] H;Hunan Agr Univ, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Nongda Rd 1, Changsha 410128, Hunan, Peoples R China.
关键词：
Coat Protein;Major Protein Band;False Smut;dsRNA Segment;Rice False Smut
摘要：
Four novel double-stranded RNA molecules, named dsRNA 1 (5124 bp), dsRNA 2(1711 bp), dsRNA 3 (1423 bp) and dsRNA 4 (855 bp), were detected in strain HNHS-1 of Ustilaginoidea virens, the causal agent of rice false smut disease. Sequence analysis showed that the dsRNA1 contains two overlapping open reading frames (ORF) potentially encoding proteins with modest levels of sequence similarity to the coat protein (CP) and putative RNA-dependent RNA polymerase (RdRp), respectively, of viruses of the family Totiviridae. The deduced gene product of the ORF encoded by dsRNA2 is homologous to putative RdRp of viruses in the family Partitiviridae; the ORF encoded by dsRNA3 shares some similarity to a hypothetical protein with unknown function. It is noteworthy that the dsRNA4 lacked integrated ORFs. Isomeric viral particles of about 40 nm in diameter were observed by transmission electron microscopy in a mycelium tissue preparation of strain HNHS-1-R1, a single-spore subculture of strain HNHS-1 containing only the dsRNA1 segment. Phylogenetic analysis and examination of the organization of the two putative RdRp sequences both indicated that there are at least two novel virus species present in strain HNHS-1. We named the two novel viruses Ustilaginoidea virens RNA virus 2 and Ustilaginoidea virens partitivirus 4, respectively.
摘要：
Leaf-color is an effective marker to identify the hybridization of rice. Leaf-color related genes function in chloroplast development and the photosynthetic pigment biosynthesis of higher plants. The ygl7 (yellow-green leaf 7) is a mutant with spontaneous yellow-green leaf phenotype across the whole lifespan but with no change to its yield traits. We cloned gene Ygl7 (Os03g59640) which encodes a magnesium-chelatase ChlD protein. Expression of ygl7 turns green-leaves to yellow, whereas RNAi-mediated silence of Ygl7 causes a lethal phenotype of the transgenic plants. This indicates the importance of the gene for rice plant. On the other hand, it corroborates that ygl7 is a non-null mutants. The content of photosynthetic pigment is lower in Ygl7 than the wild type, but its light efficiency was comparatively high. All these results indicated that the mutational YGL7 protein does not cause a complete loss of original function but instead acts as a new protein performing a new function. This new function partially includes its preceding function and possesses an additional feature to promote photosynthesis. Chl1, Ygl98, and Ygl3 are three alleles of the OsChlD gene that have been documented previously. However, mutational sites of OsChlD mutant gene and their encoded protein products were different in the three mutants. The three mutants have suppressed grain output. In our experiment, plant materials of three mutants (ygl7, chl1, and ygl98) all exhibited mutational leaf-color during the whole growth period. This result was somewhat different from previous studies. We used ygl7 as female crossed with chl1 and ygl98, respectively. Both the F1 and F2 generation display yellow-green leaf phenotype with their chlorophyll and carotenoid content falling between the values of their parents. Moreover, we noted an important phenomenon: ygl7-NIL's leaf-color is yellow, not yellowy-green, and this is also true of all back-crossed offspring with ygl7.
期刊：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2014年62(49):11924-11932 ISSN：0021-8561
通讯作者：
Chen, Li
作者机构：
[Guan, Di; Chen, Li] Chinese Acad Sci, Inst Zool, State Key Lab Integrated Management Pest Insects, Beijing 100101, Peoples R China.;[Guan, Di; Liao, Xiao-Lan] Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.;[Wang, Lei; Lu, Yong-Yue] South China Agr Univ, Red Imported Fire Ant Res Ctr, Guangzhou 510642, Guangdong, Peoples R China.
通讯机构：
[Chen, Li] C;Chinese Acad Sci, Inst Zool, State Key Lab Integrated Management Pest Insects, Beijing 100101, Peoples R China.
摘要：
A characteristic behavior in ants is to move rapidly to emission sources of alarm pheromones. The addition of ant alarm pheromones to bait is expected to enhance its attractiveness. To search for candidate compounds for bait enhancement in fire ant control, 13 related alkylpyrazine analogues in addition to synthetic alarm pheromone component were evaluated for electroantennogram (EAG) and behavioral activities in Solenopsis invicta. Most compounds elicited dose-dependent EAG and behavioral responses. There exists a correlation between the EAG and behavioral responses. Among the 14 tested alkylpyrazines, three compounds, 2-ethyl-3,6(5)-dimethyl pyrazine (1), 2,3,5-trimethylpyrazine (7), and 2,3-diethyl-5-methylpyrazine (12), elicited significant alarm responses at a dose range of 0.1-1000 ng. Further bait discovery bioassay with the three most active alkylpyrazines demonstrated that food bait accompanied by sample-treated filter paper disk attracted significantly more fire ant workers in the first 15 min period. EAG and behavioral bioassays with pure pheromone isomers accumulated by semi-preparative high-performance liquid chromatography demonstrated that 2-ethyl-3,6-dimethylpyrazine was significantly more active than 2-ethyl-3,5-dimethylpyrazine.
