浅议生猪屠宰检疫、检测和检验三者关系
作者：
郭柱良
期刊：
畜牧兽医科技信息 ,2014年(11):46-47 ISSN：1671-6027
作者机构：
湖南农业大学动物科学技术学院,长沙,410128;[郭柱良] 湖南农业大学
关键词：
生猪屠宰检疫;肉品检验;药残检测;“瘦肉精”;中毒事件;药物残留;动物疫病;安全隐患
摘要：
近几年来,河南“瘦肉精”、广州“口蹄疫”等动物疫病及药物残留中毒事件时有发生,存在肉食品安全隐患,一旦发生,会造成很大的社会影响.因此,生猪屠宰检疫、药残检测、肉品检验在把好生猪肉品上市的关键一环.下面,笔者结合在东莞实际工作情况,谈谈生猪屠宰检疫、药残检测和肉品检验这三者的关系.
1 各级政府高度重视食品安全监管工作
十八届三中全会明确提出:“健全公共安全体系.完善统一权威的食品药品安全监管机构,建立最严格的覆盖全过程的监管制度,建立食品原产地可追溯制度和质量标识制度,保障食品药品安全.”
语种：
中文
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大黄和大黄素对瘤胃还原当量生成的影响
作者：
王荣;王敏;邓近平;颜志成;谢天宇;...
作者机构：
[王荣; 王敏; 颜志成; 谢天宇; 谭支良] 中国科学院亚热带农业生态研究所,长沙410125;[王荣; 邓近平; 颜志成; 杨玲嫒] 湖南农业大学动物科学技术学院,长沙410128;[谢天宇] 内蒙古农业大学动物科学技术学院,呼和浩特010018
会议名称：
中国畜牧兽医学会动物营养学分会第七届中国饲料营养学术研讨会
会议时间：
2014-10-16
会议地点：
郑州
会议主办单位：
中国畜牧兽医学会动物营养学分会
会议论文集名称：
中国畜牧兽医学会动物营养学分会第七届中国饲料营养学术研讨会论文集
关键词：
体外模拟瘤胃发酵;甲烷减排;甲烷抑制剂
摘要：
反刍家畜瘤胃内碳水化合物和氨基酸的降解生成挥发性脂肪酸(Volatile fatty acids)伴随着还原当量(educing equivalents)的产生。瘤胃内还原当量包括NADP-H、FADH2、NADH等,而通常用氢(H)来表示。每生成1 mol乙酸和丁酸伴随生成4 mol氢,而每生成1 mol丙酸和戊酸伴随消耗2 mol氢。剩余氢在氢气生成微生物作用下生成氢气,氢气则为甲烷菌生长提供
语种：
中文
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规模化猪场配怀工作的技术要点
作者：
蒋明;陈斌
期刊：
中国猪业 ,2014年9(1):31-33 ISSN：1673-4645
作者机构：
湖南农业大学动物科学技术学院,湖南长沙,410128;[陈斌; 蒋明] 湖南农业大学
关键词：
配怀;发情鉴定;妊娠母猪;公猪;配种
摘要：
院配怀是猪场工作的一个重要环节,很大程度上决定着猪场的发展。本文结合理论和个人实践经验总结了母猪发情鉴定、妊娠期母猪和公猪的饲养管理技术,以期能为规模化猪场的配怀工作提供参考。
语种：
中文
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农业推广硕士专业学位论文选题情况调查及建议——基于湖南农业大学动物科技学院农业推广硕士（在职）养殖领域调查数据
作者：
李四元;陈清华
期刊：
中国校外教育 ,2014年(S3):351-352 ISSN：1004-8502
作者机构：
湖南农业大学动物科学技术学院
关键词：
农业推广硕士;学位论文;选题;养殖领域
摘要：
养殖领域农业推广专业硕士学位论文质量是衡量其培养质量的重要依据之一。结合湖南农业大学动物科学技术学院养殖领域农业推广专业硕士学位论文选题情况调查数据,提出了学位论文选题应结合自身专业特点、所在单位及培养单位的设备条件以及所在岗位的生产实践需求等建议。
语种：
中文
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Molecular cloning, characterization and expression analysis of trypsin-like serine protease from triangle-shell pearl mussel (Hyriopsis cumingii)
作者：
Wang, Hongquan;Liang, Jian;Zhao, Yurong;Liu, Qiaolin;Li, Yaoguo;...
期刊：
Fish & Shellfish Immunology ,2014年40(2):603-608 ISSN：1050-4648
通讯作者：
Xiao, Tiaoyi
作者机构：
[Wang, Hongquan; Xiao, Tiaoyi; Chen, Kaijian; Liu, Qiaolin; Liang, Jian; Zhao, Yurong; Li, Yaoguo] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Yi, Zili] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Xiao, Tiaoyi] H;Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.
关键词：
Triangle-shell pearl mussel;Trypsin-like serine protease;Quantitative real-time PCR;Time-course expression
摘要：
Trypsin-like serine protease (TLS) is ubiquitous in animals and plays a number of diverse roles, including dietary protein digestion, hemolymph coagulation, antimicrobial activity and immune responses, among others. This study reports the isolation of a 1048bp full-length cDNA sequence of TLS from triangle-shell pearl mussel (Hyriopsis cumingii), including a 12bp 5' UTR (untranslated region), a 172bp 3' UTR, and an open reading frame (ORF) of 864 bp by rapid amplification of cDNA ends (RACE). Bioinformatic analysis shows that the gene belongs to the trypsin-like serine protease superfamily, and contains a 15 residues N-terminal signal peptide and a conserved C-terminal domain. In comparison to other serine proteases, the catalytic triad were identified as His-98, Asp-149, and Ser-240. Quantitative real-time PCR (qPCR) showed a broad expression of the TLS gene in ten tested tissues. Time-course expression analysis demonstrated that the expression level of the TLS mRNA was significantly up-regulated in eight tested tissues (liver, intestine, gill, heart, axe foot, adductor muscle, kidney and gonad), but down-regulated in mantle and stomach after Aeromonas hydrophi l a injection. This is one of the results indicate that TLS may be involved in innate defense reactions against A.hydrophila in triangle-shell pearl mussel. © 2014 Elsevier Ltd.
语种：
英文
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减少生猪粪氮磷排放措施的研究
作者：
李孟伟;陈清华
期刊：
中国饲料添加剂 ,2014年(1):25-27 ISSN：1729-6013
作者机构：
湖南农业大学动物科学技术学院
关键词：
养猪污染;氮磷排放;环保饲粮
摘要：
近年来我国生猪养殖集约化程度日益提高，养猪生产的环境污染问题日益突出，新型环保猪饲料的研发与推广成为我国饲料工业的重要发展方向。本文旨在阐述生猪养殖中氮磷污染现状，根据氮磷元素在猪体内的代谢原理，探讨通过改变饲料配制方式达到从源头减少氮磷污染物排放的目的。其主要措施是通过氨基酸平衡配制饲粮和开发应用加酶饲粮。
语种：
中文
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维生素E在水产动物中的应用研究进展
作者：
孙念;李伟;李迪;何美凤;黎玉元
期刊：
中国饲料添加剂 ,2014年(5):1-4 ISSN：1729-6013
作者机构：
湖南农业大学动物科学技术学院,长沙410128;[何美凤; 孙念; 黎玉元; 李迪; 李伟] 湖南农业大学
关键词：
维生素E;水产动物;研究进展
摘要：
本文综述了维生素E对水产动物生长性能、消化系统、抗氧化能力、免疫功能的影响，为维生素E在水产动物饲料中的开发利用提供参考。
语种：
中文
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Effects of practical dietary protein to lipid levels on growth, digestive enzyme activities and body composition of juvenile rice field eel (Monopterus albus)
作者：
Ma, Xiao;Hu, Yi* ;Wang, Xiao-Qing;Ai, Qing-Hui;He, Zhi-Gang;...
期刊：
Aquaculture International ,2014年22(2):749-760 ISSN：0967-6120
通讯作者：
Hu, Yi
作者机构：
[Hu, Yi; Wang, Xiao-Qing; Ma, Xiao] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Feng, Fu-Xian; Ai, Qing-Hui] Ocean Univ China, Key Lab Mariculture, Minist Educ, Qingdao 266003, Shandong, Peoples R China.;[He, Zhi-Gang] Fisheries Res Inst Hunan Prov, Changsha 410000, Hunan, Peoples R China.;[Lu, Xiang-Yang] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Hu, Yi] H;Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.
关键词：
Monopterus albus;Growth;Protein;Lipid;Digestive enzyme
摘要：
A 10-week feeding experiment was conducted to evaluate the effects of dietary protein to lipid ratios (P/L) on growth, intestinal digestive enzyme activities and body composition in juvenile rice field eel (Monopterus albus) (initial mean body weight of 65.76 ± 1.07 g, mean ± SEM). Nine test diets were formulated in a 3 × 3 factorial design to contain three protein levels (350, 400 and 450 g kg-1) for each of three lipid levels (40, 80 and 120 g kg-1), respectively. Each diet was randomly assigned to triplicate groups of 60 fish per net cage (1.5 × 2.0 × 1.5 m). Results showed the survival was above 96 % and was not affected by dietary treatments. Fish fed the diet with 450 g kg-1 protein and 40 g kg-1 lipid showed the best weight gain (WG) (103.95 %) and feed conversion ratio (1.60) (P < 0.05). WG, protein efficiency ratio and energy retention increased with the increasing in lipid at 350 g kg-1 protein level (P < 0.05). However, WG showed a little decline with increasing dietary lipid when fish fed the diets with 400 and 450 g kg-1 protein level, but no significant difference was observed (P < 0.05). Hepatosomatic index, visceralsomatic index and intestinal lipase activity increased with the increasing of dietary lipid level irrespective protein level. Intestinal trypsin activity increased with the increasing of dietary lipid level when fish fed the diets with 350 g kg-1 protein, but showed converse trend when fish fed the diets with 400 and 450 g kg-1 protein. Serum triglyceride, body lipid and energy were positively correlated with the dietary lipid. Results of the present study showed that the dietary protein/lipid ratio of 450/40 g kg-1 is considered optimum for rice field eel under culture conditions, and the increase in dietary lipid level has no efficient protein-sparing effect when fish fed the diets with 400 and 450 g kg-1 protein level. © 2013 Springer Science+Business Media Dordrecht.
语种：
英文
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鮰爱德华氏菌研究进展
作者：
谢建洋;王荣华;刘小燕;谭情
期刊：
科学养鱼 ,2014年(8):54-55,中插1 ISSN：1004-843X
作者机构：
[谢建洋] 安化县畜牧水产局;湖南农业大学动物科学技术学院;[刘小燕; 王荣华; 谭情] 湖南农业大学
关键词：
爱德华氏菌;斑点叉尾;变形菌;弓背鱼;南方鲇;日本鳗鲡;肠杆菌科;云斑;斑马鱼;致病性
摘要：
(鮰)爱德华氏菌(Edwardsiella ictaluri)是水生动物的一种常见高死亡率的致病菌,其分类地位为变形菌门、γ-变形菌纲、肠杆菌目、肠杆菌科、爱德华氏菌属.该致病菌对云斑(鮰)、蓝色弓背鱼、斑马鱼、虹鳟、黄颡鱼、日本鳗鲡、南方鲇都有致病性,对斑点叉尾(鮰)可引起斑点叉尾(鮰)肠道败血症.被(鮰)爱德华氏菌感染的斑点叉尾(鮰)食欲不振、甚至禁食,因而抗生素药物对于斑点叉尾(鮰)肠败血症的治疗效果不显著,每年斑点叉尾(鮰)大面积死亡给全球斑点叉尾(鮰)产业带来巨大的经济损失.我们就目前国内外有关(鮰)爱德华氏菌的研究现状从生物学特性、感染症状、致病机理及毒力因子、预防与治疗措施等方面进行回顾和总结,为预防与治疗(鮰)爱德华氏菌病提供一定的理论依据.
语种：
中文
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Oral MSG administration alters hepatic expression of genes for lipid and nitrogen metabolism in suckling piglets
作者：
Chen, Gang;Zhang, Jun;Zhang, Yuzhe;Liao, Peng;Li, Tiejun;...
期刊：
Amino Acids ,2014年46(1):245-250 ISSN：0939-4451
通讯作者：
Yin, Yulong
作者机构：
[Zhang, Yuzhe; Chen, Gang; Li, Tiejun; Yin, Yulong; Zhang, Jun; Liao, Peng] Chinese Acad Sci, Key Lab Agroecol & Proc Subtrop Reg,Inst Subtrop, Res Ctr Hlth Breeding Livestock & Poultry,Minist, Hunan Engn & Res Ctr Anim & Poultry Sci,Sci Obser, Changsha 410125, Hunan, Peoples R China.;[Chen, Lixiang; Chen, Gang] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Wang, Jinquan] Chinese Acad Agr Sci, Feed Res Inst, Beijing 100098, Peoples R China.;[Wu, Guoyao] China Agr Univ, State Key Lab Anim Nutr, Beijing 100193, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构：
[Yin, Yulong] C;Chinese Acad Sci, Key Lab Agroecol & Proc Subtrop Reg,Inst Subtrop, Res Ctr Hlth Breeding Livestock & Poultry,Minist, Hunan Engn & Res Ctr Anim & Poultry Sci,Sci Obser, Changsha 410125, Hunan, Peoples R China.
关键词：
Monosodium glutamate;Pig;Liver;Gene expression
摘要：
This experiment was conducted to investigate the effects of oral administration of monosodium glutamate (MSG) on expression of genes for hepatic lipid and nitrogen metabolism in piglets. A total of 24 newborn pigs were assigned randomly into one of four treatments (n = 6/group). The doses of oral MSG administration, given at 8:00 and 18:00 to sow-reared piglets between 0 and 21 days of age, were 0 (control), 0.06 (low dose), 0.5 (intermediate dose), and 1 (high dose) g/kg body weight/day. At the end of the 3-week treatment, serum concentrations of total protein and high-density lipoprotein cholesterol in the intermediate dose group were elevated than those in the control group (P < 0.05). Hepatic mRNA levels for fatty acid synthase, acetyl-coA carboxylase, insulin-like growth factor-1, glutamate-oxaloacetate transaminase, and glutamate-pyruvate transaminase were higher in the middle-dose group (P < 0.05), compared with the control group. MSG administration did not affect hepatic mRNA levels for hormone-sensitive lipase or carnitine palmitoyl transferase-1. We conclude that oral MSG administration alters hepatic expression of certain genes for lipid and nitrogen metabolism in suckling piglets. © 2013 Springer-Verlag Wien.
语种：
英文
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n-6:n-3 PUFA ratio is involved in regulating lipid metabolism and inflammation in pigs
作者：
Duan, Yehui;Li, Fengna* ;Li, Lili;Fan, Juexin;Sun, Xiaoming;...
期刊：
British Journal of Nutrition ,2014年111(3):445-451 ISSN：0007-1145
通讯作者：
Li, Fengna
作者机构：
[Li, Lili; Duan, Yehui; Li, Fengna; Sun, Xiaoming; Yin, Yulong] Chinese Acad Sci, Key Lab Agroecol Proc Subtrop Reg, Hunan Prov Engn Res Ctr Hlth Livestock,Minist Agr, Inst Subtrop Agr,Sci Observing & Expt Stn Anim Nu, Changsha 410125, Hunan, Peoples R China.;[Duan, Yehui] Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410005, Hunan, Peoples R China.;[Fan, Juexin] Hunan Agr Univ, Coll Anim Sci, Changsha 410128, Hunan, Peoples R China.;[Sun, Xiaoming] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China.;[Li, Fengna] Chinese Acad Sci, Key Lab Agroecol Proc Subtrop Reg, Hunan Prov Engn Res Ctr Hlth Livestock,Minist Agr, Inst Subtrop Agr,Sci Observing & Expt Stn Anim Nu, 644 Yuanda Rd, Changsha 410125, Hunan, Peoples R China.
通讯机构：
[Li, Fengna] C;Chinese Acad Sci, Key Lab Agroecol Proc Subtrop Reg, Hunan Prov Engn Res Ctr Hlth Livestock,Minist Agr, Inst Subtrop Agr,Sci Observing & Expt Stn Anim Nu, 644 Yuanda Rd, Changsha 410125, Hunan, Peoples R China.
关键词：
n-6:n-3 ratios;PUFA;Lipid metabolism;Inflammation;Pigs
摘要：
The objective of the present study was to investigate the optimal dietary n-6:n-3 PUFA ratios that regulate lipid metabolism and inflammation in pigs. A total of ninety-six cross-bred (Large White × Landrace) growing-finishing pigs (73·8 (sem 1·6) kg) were chosen and fed one of the four isoenergetic diets with n-6:n-3 PUFA ratios of 1:1, 2·5:1, 5:1 and 10:1. The growth performance of pigs fed the diet with an n-6:n-3 PUFA ratio of 5:1 was the best, but the group fed the diet with an n-6:n-3 PUFA ratio of 1:1 had the highest muscle mass and the lowest adipose tissue mass (P< 0·05). The concentrations of IL-6 and IL-1β of pigs fed the diet with an n-6:n-3 PUFA ratio of 1:1 were decreased compared with those of the other groups (P< 0·05). The concentration of adiponectin of pigs fed the diet with an n-6:n-3 PUFA ratio of 1:1 was also markedly decreased, but the concentration of leptin was increased compared with that of the groups fed the diets with n-6:n-3 PUFA ratios of 5:1 and 10:1 (P< 0·05). Additionally, the optimal dietary ratios of n-6:n-3 PUFA of 1:1 and 5:1 markedly suppressed the expression levels of lipid metabolism-related genes and proteins such as phosphoinositide-3-kinase-α, fatty acid transport protein-1 and PPARγ. They also significantly suppressed the expression levels of the inflammatory cytokines IL-1β, TNF-α and IL-6. The results indicated that the optimal n-6:n-3 PUFA ratios of 1:1 and 5:1 exerted beneficial effects on lipid metabolism and inflammatory system, leading to the availability of more energy and nutrients for high performance and homeostatic pathways. © 2013 The Authors.
语种：
英文
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不同有机硒源对鹌鹑肉、蛋中硒含量及血清生化指标的影响
作者：
曲湘勇;蔡超;魏艳红;袁小平;孙安权
期刊：
营养学报 ,2014年36(4):395-397 ISSN：0512-7955
作者机构：
[曲湘勇; 蔡超; 魏艳红] 湖南农业大学动物科学技术学院;[袁小平] 长沙扬航农业科技有限公司;[孙安权] 奥格生物技术(上海)有限公司
关键词：
纳米硒;酵母硒;硒含量;血清生化指标
摘要：
硒(selenium)是动物必需的微量元素,它参与动物生命维持、生长、繁殖、免疫等生理代谢。人们日常饮食中的硒含量低于人体的正常需要,研究表明,如果提高动物产品的硒含量,不仅可改善动物的健康状况,还能提高动物产品(肉、乳和蛋品等)的价值。但当前饲料中普遍使用的硒源亚硒酸钠存在吸收率低、过氧化作用及潜在的污染等问题。酵母硒(selenium yeast,SY)与纳米硒(nano-selenium,nano-Se)是当前开发的优质有机硒源,酵母硒中的有机硒易被机体利用,并可以通过Na+依赖性氨基酸途径被主动地吸收,吸收率高,并且营养丰富;而纳米硒具有特有的纳米颗粒性,其吸收率高、生物活性高、安全性高。两者在家禽生产上已取得了一定效果,但在鹌鹑生产中的应用效果及其比较还未见报道。本试验在鹌鹑日粮中添加不同水平酵母硒和纳米硒探讨二者对鹌鹑肉与蛋中硒含量、血清生化指标的影响,为高效、安全硒源研究与应用提供实验依据。
语种：
中文
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Resveratrol induces antioxidant and heat shock protein mRNA expression in response to heat stress in black-boned chickens
作者：
Liu, L. L.;He, J. H.* ;Xie, H. B.;Yang, Y. S.;Li, J. C.;...
期刊：
Poultry Science ,2014年93(1):54-62 ISSN：0032-5791
通讯作者：
He, J. H.
作者机构：
[Li, J. C.; Zou, Y.; He, J. H.; Liu, L. L.; Xie, H. B.; Yang, Y. S.] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Liu, L. L.] Hunan Univ Sci & Technol, Coll Life Sci, Xiangtan 411201, Peoples R China.
通讯机构：
[He, J. H.] H;Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.
关键词：
antioxidant response;black-boned chicken;heat stress;heat shock protein;resveratrol
摘要：
This study investigated the effects of dietary resveratrol at 0, 200, 400, or 600 mg/kg of diet on the performance, immune organ growth index, serum parameters, and expression levels of heat shock protein (Hsp) 27, Hsp70, and Hsp90 mRNA in the bursa of Fabricius, thymus, and spleen of 42-d-old female blackboned chickens exposed to heat stress at 37 ± 2°C for 15 d. The results showed that heat stress reduced daily feed intake and BW gain; decreased serum glutathione (GSH), growth hormone, and insulin-like growth factor- 1 levels; and inhibited GSH peroxidase (GSH-Px), superoxide dismutase (SoD), and catalase (CAT) activities compared with birds subjected to thermo-neutral circumstances. Chickens that were fed diets supplemented with resveratrol exhibited a linear increase in feed intake and BW gain (P < 0.001); serum GSH, growth hormone, and insulin-like growth factor-1 levels (P ≤ 0.01); and GSH-Px, SoD, and CAT activities (P < 0.001) compared with chickens that were fed diets without resveratrol during heat stress. In contrast, serum malonaldehyde concentrations were decreased (P < 0.001) in the chickens fed a resveratrol-supplemented diet. Heat stress also reduced (P < 0.05) the growth index of the bursa of Fabricus and spleen; however, it had no effect on the growth index of the thymus. The growth index of the bursa of Fabricius and spleen increased (P < 0.05) upon heat stress and coincided with an increase in supplemental resveratrol levels. The expression of Hsp27, Hsp70, and Hsp90 mRNA in the bursa of Fabricius and spleen were increased (P < 0.01), but those of Hsp27 and Hsp90 mRNA in thymus were decreased (P < 0.01) under heat stress compared with no heat stress. Resveratrol attenuated the heat stress-induced overexpression of Hsp27, Hsp70, and Hsp90 mRNA in the bursa of Fabricius and spleen and increased the low expression of Hsp27 and Hsp90 mRNA in thymus upon heat stress. The results suggest that supplemental resveratrol improves growth performance and reduces oxidative stress in heat-stressed black-boned chickens by increasing serum growth hormone concentrations and modulating the expression of heat shock genes in organs of the immune system. © 2014 Poultry Science Association Inc.
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英文
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Effects of Chitosan on Intestinal Inflammation in Weaned Pigs Challenged by Enterotoxigenic Escherichia coli
作者：
Xiao, Dingfu;Wang, Yongfei;Liu, Gang;He, Jianhua;Qiu, Wei;...
期刊：
PLOS ONE ,2014年9(8):e104192 ISSN：1932-6203
通讯作者：
Tang, Zhiru
作者机构：
[Ran, Maoliang; Xiao, Dingfu; He, Jianhua] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha, Hunan, Peoples R China.;[Wang, Yongfei; Feng, Zemeng] Twins Grp Co Ltd, Ctr Res & Dev, Nanchang, Jiangxi, Peoples R China.;[Liu, Gang; Yin, Yulong] Chinese Acad Sci, Inst Subtrop Agr, Hunan Engn & Res Ctr Anim & Poultry Sci, Changsha, Hunan, Peoples R China.;[Liu, Gang; Yin, Yulong] Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agro ecol Processes Subtrop Reg, Changsha, Hunan, Peoples R China.;[Qiu, Wei] Hunan New Wellful Co LTD, Changsha, Hunan, Peoples R China.
通讯机构：
[Tang, Zhiru] S;Southwest Univ, Coll Anim Sci & Technol, Chongqing, Peoples R China.
关键词：
Swine;Diarrhea;Diet;Jejunum;Cytokines;Inflammation;Inflammatory bowel disease;Protein expression
摘要：
The aim of this study was to investigate whether supplementation with chitosan (COS) could reduce diarrhea and to explore how COS alleviates intestinal inflammation in weaned pigs. Thirty pigs (Duroc×Landrace×Yorkshire, initial BW of 5.65±0.27) weaned at age 21 d were challenged with enterotoxigenic Escherichia coli during a preliminary trial period, and then divided into three treatment groups. Pigs in individual pens were fed a corn-soybean meal diet, that contained either 0 (control), 50 mg/kg chlortetracycline, or 300 mg/kg COS for 21 days. The post-weaning diarrhea frequency, calprotectin levels and TLR4 protein expression were decreased (P<0.05) in both the COS and chlortetracycline groups compared with control. Simultaneously, supplemental COS and chlortetracycline had no effect on the mRNA expression of TNF-α in the jejunal mucosa, or on the concentrations of IL-1β, IL-6 and TNF-α in serum. However, COS supplementation improved (P<0.05) the mRNA expression of IL-1β and IL-6 in the jejunal mucosa. The results indicate that supplementation with COS at 300 mg/kg was effective for alleviating intestinal inflammation and enhancing the cell-mediated immune response. As feed additives, chitosan and chlortetracycline may influence different mechanisms for alleviating inflammation in piglets.
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英文
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Development and characterization of 17 microsatellite markers for Chinese soft-shelled turtle, Pelodiscus sinensis
作者：
Ma, Xiao;Xiong, Gang;Wang, Luming;Yan, Luqi;Li, Xuejun;...
期刊：
CONSERVATION GENETICS RESOURCES ,2014年6(4):955-956 ISSN：1877-7252
通讯作者：
Wang, Xiaoqing
作者机构：
[Xiong, Gang; Wang, Luming; Yan, Luqi; Ma, Xiao; Wang, Xiaoqing] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Li, Xuejun; Xie, Dizhi; Ma, Xiao] Henan Normal Univ, Coll Fisheries, Xinxiang 453007, Henan, Peoples R China.;[Xiong, Gang] Hunan Biol Electromech Vocat & Tech Coll, Changsha 410127, Hunan, Peoples R China.
通讯机构：
[Wang, Xiaoqing] H;Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.
关键词：
Pelodiscus sinensis;Chinese soft-shelled turtle;Microsatellite;Next generation sequencing
摘要：
A set of 17 polymorphic microsatellite markers were isolated and characterized for the Chinese soft-shelled turtle (Pelodiscus sinensis) using a next generation sequencing approach. The number of alleles per locus ranged from 2 to 11. The observed, expected heterozygosities and polymorphic information content per locus varied from 0.103 to 0.750, 0.222 to 0.883 and 0.158 to 0.788, respectively. Significant linkage disequilibrium and deviations from the Hardy–Weinberg disequilibrium between two pairs of loci were found. These loci will be beneficial for the population genetic structure investigation and marker assisted breeding for P. sinensis. © 2014, Springer Science+Business Media Dordrecht.
语种：
英文
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公猪生殖系统的氧化损伤及酸性氨基酸的干预效应
作者：
路露;龙江松;李铁军;邓近平
作者机构：
[路露; 龙江松; 邓近平] 湖南农业大学动物科学技术学院 长沙410128;[李铁军] 中国科学院亚热带农业生态研究所,长沙4101251
会议名称：
中国畜牧兽医学会动物繁殖学分会第十七届学术研讨会
会议时间：
2014-8-1
会议地点：
福州
会议主办单位：
中国畜牧兽医学会动物繁殖学分会
会议论文集名称：
中国畜牧兽医学会动物繁殖学分会第十七届学术研讨会论文集
语种：
中文
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Effects of orally administered Escherichia coli Nissle 1917 on growth performance and jejunal mucosal membrane integrity, morphology, immune parameters and antioxidant capacity in early weaned piglets
作者：
Deng, H.;Xu, L.;Tang, Z. R.* ;Lai, X.;Xiao, D. H.;...
期刊：
Animal Feed Science and Technology ,2014年198:286-294 ISSN：0377-8401
通讯作者：
Tang, Z. R.
作者机构：
[Zhang, X. L.; Lai, X.; Tang, Z. R.; Sun, W. Z.; Shi, B. S.; Xu, L.; Sun, Z. H.; Deng, H.; Zhang, Z.] Southwest Univ, Key Lab Biofeed & Anim Nutr, Chongqing 400715, Peoples R China.;[Xiao, D. H.] Hunan Agr Univ, Acad Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Tang, Z. R.] Southwest Univ, Coll Anim Sci & Technol, Tiansheng Rd 2, Chongqing 400715, Peoples R China.
通讯机构：
[Tang, Z. R.] S;Southwest Univ, Coll Anim Sci & Technol, Tiansheng Rd 2, Chongqing 400715, Peoples R China.
关键词：
Escherichia coli Nissle 1917;Inflammatory cytokines;Intestinal epithelial barrier;Probiotic;Toll-like receptor 4;Weaned piglets
摘要：
The objective of this study was to assess the effect of orally administered Escherichia coli Nissle 1917 (EcN) on the jejunal health of weaned piglets that were unchallenged or challenged with porcine enterotoxigenic E. coli Abbottstown (EcA). A total of 40 weaned Landrace x Yorkshire piglets (5.83 +/- 0.24 kg) were allocated to 4 groups with 10 barrows per group, following a 2 x 2 factorial design with 2 inclusion levels of EcN (daily oral administration of 2 mL sterile water or 2 mL EcN (10(9) cells/mL)) at 10:00 h) and 2 inclusion levels of EcA (daily oral administration of 2 mL sterile water or 2 mL EcA (10(9) cells/mL) at 14:00 h). Thus, there were four treatments: (1) control diet (C); (2) C + EcN (N); (3) C + EcA (A); (4) C + EcN + EcA (N+ A). This experiment lasted for 21 d. The results indicated a significant interaction between EcN and EcA on growth performance and jejuna( mucosa( membrane integrity, morphology, immune parameters and the antioxidant capacity in 21-day-weaned piglets (P<0.05). The results that piglets in the A group had lowest feed conversion, daily weight gain, daily feed intake, jejunal villus height (P<0.05) among the 4 groups suggested that the oral administration of EcA decreased growth performance. The results that piglets in the A group had lowest mRNA levels of regenerating islet derived protein 3 gamma and transforming growth factor beta 1 (P<0.05), lowest protein levels of claudin-1, occludin, and zonula occludens-1 (P<0.05) and highest serum D-lactate concentration (P<0.05) among the 4 groups suggested that the oral administration of EcA damaged jejunal mucosal membrane integrity. The immune and antioxdant parameters results that orally administered EcA increased mRNA levels of interleukin (IL)-1 beta, IL-8, tumor necrosis factor (TNF)-alpha, and toll-like receptor (TLR)-4 (P<0.05), increased the concentrations of IgM, IgG, IL-1 beta, TNF-alpha, sIgA (P<0.05), and TLR-4, and decreased activities of superoxide dismutase and total antioxidant (P<0.05) suggested that EcA could make the jejunal mucosal membrane inflamed in early weaned piglets. Orally administered EcN tended to improved growth performance, jejunal villus height, the immune and antioxdant function, jejunal mucosal membrane integrity in piglet unchallenged or challenged with EcA. This improvement of the jejunal health and growth performance of piglets challenged with EcA by oral administration of EcN could be attributed to increased defense against harmful bacteria and improved epithelial barrier function. These results demonstrated that EcN could be used in early-weaned piglets to prevent intestinal infection by the pathogenic bacteria EcA. (C) 2014 Published by Elsevier B.V.
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英文
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河蚬提取物对芙蓉鲤鲫生长、脂代谢及抗氧化的影响
作者：
马行厚;皮杰;张健;李德亮;肖调义
作者机构：
[马行厚; 皮杰; 张健; 李德亮; 肖调义] 湖南农业大学动物科学技术学院
会议名称：
2014年中国水产学会学术年会
会议时间：
2014-01-01
会议地点：
中国湖南长沙
会议论文集名称：
2014年中国水产学会学术年会论文摘要集
关键词：
河蚬提取物;芙蓉鲤鲫;生长;脂代谢;抗氧化
摘要：
本文研究河蚬提取物(FCE)对芙蓉鲤鲫(1.69±0.05g)生长、脂类代谢及抗氧化的影响。实验饲料以基础饲料为对照,通过在基础饲料中分别添加3.2 g/kg、6.4 g/kg、12.8 g/kg FCE的方式获得。结果显示,各实验组与对照组之间增重率、脏体比均无显著差异(p>0.05),但实验组肝体比均显著低于对照组(p<0.05),6.4 g/kg和12.8 g/kg组肥满度显著低于对照组(p
语种：
中文
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日粮赖氨酸水平对1218周龄雌性临武鸭血液生化指标的影响
作者：
林谦;戴求仲;蒋桂韬;张旭;王向荣
作者机构：
[林谦; 戴求仲; 蒋桂韬; 张旭; 王向荣] 湖南省畜牧兽医研究所动物营养与饲养技术研究室,长沙410131;[林谦; 戴求仲] 湖南农业大学动物科学技术学院,长沙410128
会议名称：
中国畜牧兽医学会动物营养学分会第七届中国饲料营养学术研讨会
会议时间：
2014-10-16
会议地点：
郑州
会议主办单位：
中国畜牧兽医学会动物营养学分会
会议论文集名称：
中国畜牧兽医学会动物营养学分会第七届中国饲料营养学术研讨会论文集
关键词：
后备期;临武鸭;赖氨酸水平;血液生化指标
摘要：
为研究日粮不同赖氨酸(Lys)水平对12~18周龄雌性临武鸭血液生化指标的影响,试验选取健康、体重接近的84d雌性临武鸭210羽,采用单因素试验设计,随机分为5个处理(饲粮以小麦、玉米、豆粕、次粉为主要原料配合而成,设5个赖氨酸水平,分别为0.55%、0.65%、0.75%、0.85%、0.95%),每处理设6个重复,每重复7只鸭,试验全期为42 d。结果显示:①日粮赖氨酸水平显著或极显著影响试鸭
语种：
中文
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灭活草分枝杆菌免疫调节研究进展
作者：
钟金凤;方热军
期刊：
免疫学杂志 ,2014年30(11):1011-1016 ISSN：1000-8861
作者机构：
410128长沙,湖南农业大学大学动物科技学院;421005衡阳,湖南环境生物职业技术学院生物工程学院;[方热军; 钟金凤] 湖南农业大学
关键词：
草分枝杆菌;免疫调节;作用机制
摘要：
灭活草分枝杆菌是一种十分有潜力的免疫调节剂。本文从草分枝杆菌的培养特性及菌体组成出发,全面概括草分枝杆菌的特点,并从免疫调节对象、作用方式、活性成分确定及作用机制等方面进行系统阐述。
语种：
中文
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