期刊：
Journal of Advanced Research,2025年70:323-338 ISSN：2090-1232
通讯作者：
Xuan Zhao<&wdkj&>Miaomiao Wu<&wdkj&>Yaoyao Xia
作者机构：
[Li, Yikun; Chen, Yating; "Liu, Yun] College of Animal Science and Technology, Southwest University, Chongqing 400715, China;[Wu, Zifang] College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China;[Li, Yikun] College of Animal Science, South China Agricultural University, Guangzhou, Guangdong 510642, China;[Zhao, Xuan] College of Animal Science and Technology, Southwest University, Chongqing 400715, China. Electronic address: zhaoxuann@swu.edu.cn;[Wu, Miaomiao] Animal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128, China. Electronic address: miaomiaowu0316@hunau.edu.cn
通讯机构：
[Xuan Zhao; Yaoyao Xia] C;[Miaomiao Wu] A;College of Animal Science and Technology, Southwest University, Chongqing 400715, China<&wdkj&>Animal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128, China
摘要：
Background Endothelial cell (EC) metabolism plays a crucial role in the process of angiogenesis. Intrinsic metabolic events such as glycolysis, fatty acid oxidation, and glutamine metabolism, support secure vascular migration and proliferation, energy and biomass production, as well as redox homeostasis maintenance during vessel formation. Nevertheless, perturbation of EC metabolism instigates vascular dysregulation-associated diseases, especially cancer.
Endothelial cell (EC) metabolism plays a crucial role in the process of angiogenesis. Intrinsic metabolic events such as glycolysis, fatty acid oxidation, and glutamine metabolism, support secure vascular migration and proliferation, energy and biomass production, as well as redox homeostasis maintenance during vessel formation. Nevertheless, perturbation of EC metabolism instigates vascular dysregulation-associated diseases, especially cancer.
Aim of review In this review, we aim to discuss the metabolic regulation of angiogenesis by EC metabolites and metabolic enzymes, as well as prospect the possible therapeutic opportunities and strategies targeting EC metabolism.
In this review, we aim to discuss the metabolic regulation of angiogenesis by EC metabolites and metabolic enzymes, as well as prospect the possible therapeutic opportunities and strategies targeting EC metabolism.
Key scientific concepts of review In this work, we discuss various aspects of EC metabolism considering normal and diseased vasculature. Of relevance, we highlight that the implications of EC metabolism-targeted intervention (chiefly by metabolic enzymes or metabolites) could be harnessed in orchestrating a spectrum of pathological angiogenesis-associated diseases.
In this work, we discuss various aspects of EC metabolism considering normal and diseased vasculature. Of relevance, we highlight that the implications of EC metabolism-targeted intervention (chiefly by metabolic enzymes or metabolites) could be harnessed in orchestrating a spectrum of pathological angiogenesis-associated diseases.
作者机构：
[Yang, Hong; Fu, Jiaojiao; Zhang, Mengyuan; Cao, Xinyi; Xiao, Yu] Fisheries College, Hunan Agricultural University, Changsha 410128, China;Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization, Hunan Agricultural University, Changsha 410128, China;[Wang, Hongquan; Xiao, Tiaoyi; Lv, Zhao] Fisheries College, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization, Hunan Agricultural University, Changsha 410128, China
通讯机构：
[Zhao Lv] F;Fisheries College, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization, Hunan Agricultural University, Changsha 410128, China
摘要：
IL-10, an anti-inflammatory cytokine, plays crucial roles in the context of infectious inflammatory diseases, whose gene variations and expression are tightly linked to disease resistance in animals. However, there is limited research on the genetic variations governing IL-10 expression and the underlying effects in fish. In this study, we identified a GAGTT insertion/deletion (Ins/Del) variation within the IL-10 gene promoter and analyzed the effects of this variation on IL-10 expression, inflammatory response and its association with disease resistance in grass carp. With dual luciferase reporter assays, we demonstrated that the GAGTT Ins/Del variation significantly altered the IL-10 promoter activity in grass carp. DNA pull-down combined with mass spectrometry revealed that the spectrum of transcription factors that bind to IL-10 promoter variants caused by GAGTT Ins/Del variation was quite different. Moreover, three representative transcription factors, JunD/AP-1, p50/NF-κB, and HMGB1, differentially transactivated grass carp IL-10 promoter variants, as confirmed by western blotting and dual luciferase reporter assays. Using the GAGTT/− genotype of the heterozygous grass carp parents, we obtained full-sib family offspring of the GAGTT/GAGTT, GAGTT/−, and −/− genotypes. During grass carp reovirus (GCRV) infection, these three IL-10 genotypes of grass carp offspring displayed different survival rates. Importantly, grass carp with the GAGTT/− genotype had greater survival than those with the other two genotypes after GCRV infection. Furthermore, the results indicated that the improved intestinal inflammation at 1 d post-GCRV infection and enhanced immunocompetence in the spleen at 5 d post-GCRV infection may contribute to the disease resistance of grass carp with the GAGTT/− genotype. Overall, this study offers new insight into the inflammatory and immune regulatory mechanisms specific to IL-10 from a genetic perspective in fish and may provide a valuable molecular marker for breeding disease-resistant grass carp.
IL-10, an anti-inflammatory cytokine, plays crucial roles in the context of infectious inflammatory diseases, whose gene variations and expression are tightly linked to disease resistance in animals. However, there is limited research on the genetic variations governing IL-10 expression and the underlying effects in fish. In this study, we identified a GAGTT insertion/deletion (Ins/Del) variation within the IL-10 gene promoter and analyzed the effects of this variation on IL-10 expression, inflammatory response and its association with disease resistance in grass carp. With dual luciferase reporter assays, we demonstrated that the GAGTT Ins/Del variation significantly altered the IL-10 promoter activity in grass carp. DNA pull-down combined with mass spectrometry revealed that the spectrum of transcription factors that bind to IL-10 promoter variants caused by GAGTT Ins/Del variation was quite different. Moreover, three representative transcription factors, JunD/AP-1, p50/NF-κB, and HMGB1, differentially transactivated grass carp IL-10 promoter variants, as confirmed by western blotting and dual luciferase reporter assays. Using the GAGTT/− genotype of the heterozygous grass carp parents, we obtained full-sib family offspring of the GAGTT/GAGTT, GAGTT/−, and −/− genotypes. During grass carp reovirus (GCRV) infection, these three IL-10 genotypes of grass carp offspring displayed different survival rates. Importantly, grass carp with the GAGTT/− genotype had greater survival than those with the other two genotypes after GCRV infection. Furthermore, the results indicated that the improved intestinal inflammation at 1 d post-GCRV infection and enhanced immunocompetence in the spleen at 5 d post-GCRV infection may contribute to the disease resistance of grass carp with the GAGTT/− genotype. Overall, this study offers new insight into the inflammatory and immune regulatory mechanisms specific to IL-10 from a genetic perspective in fish and may provide a valuable molecular marker for breeding disease-resistant grass carp.
摘要：
This study aimed to investigate the effects of dietary isatidis root polysaccharide (IRP) on diarrhea, immunity, and intestinal health in weanling piglets. Forty healthy piglets were randomly assigned to five groups receiving varying dosages of IRP. The findings indicated that different concentrations of IRP significantly reduced diarrhea scores (p < 0.01). Notably, the serum levels of immunoglobulin A and immunoglobulin G increased linearly and quadratically (p < 0.01), while immunoglobulin M also showed a linear increase (p < 0.05) in IRP-fed piglets. The secretory immunoglobulin A levels in ileal contents were significantly higher compared to control piglets (p < 0.01). Key intestinal health parameters, including villus height, villus height-to-crypt depth ratio, and goblet cell numbers, showed linear and quadratic increases in both the jejunum and ileum (p < 0.05), while crypt depth decreased significantly (p < 0.01). Additionally, the expression of IL-10, ZO-1, occludin, and mucin2 was upregulated linearly and quadratically in IRP-fed piglets (p < 0.05). In cultured IPEC-J2 cells, ZO-1 and occludin expression levels significantly increased upon exposure to 400 μg/mL IRP (p < 0.01). Furthermore, the relative abundances of Escherichia coli, Ralstonia pickettii, and Desulfovibrio fairfieldensis decreased linearly with increasing dietary IRP concentration. In conclusion, IRP shows promise as an effective dietary supplement for mitigating diarrhea and enhancing intestinal health in early weaned piglets.
摘要：
Phytosterols (PSs) are widely present in plants, particularly abundant in plant oils and seeds. PSs are reported to have various biological activities, such as lowering cholesterol, alongside antioxidant and antibacterial activities. This research examined the effects of PSs in finishing pigs, including growth performance, serum biochemistry, and fecal bacteria. Two treatment groups (each treatment group consisted of five biological replicates, and each replicate comprised five pigs housed communally) were randomly assigned to the fifty finishing pigs (equally divided by sex) of "Duroc × Landrace × Yorkshire" three-way cross with 79.76 ± 1.29 (kg) body weight. The control group (CON) was given basic food, while the experimental group was given basic food containing 300 mg PS/kg (PS). Dietary PS supplementation markedly raised the levels of average daily feed intake (ADFI) and apparent digestibility of dry matter (DM), crude protein (CP), ether extract (EE), and gross energy (GE) in comparison to the CON (p < 0.05). Additionally, PSs also significantly boosted the concentrations of high-density lipoprotein cholesterol (HDL-C), total protein (TP), catalase (CAT), superoxide dismutase (SOD), total antioxidant capacity (T-AOC), immunoglobulin G (IgG), motilin (MTL), and glucagon-like peptide-1 (GLP-1) (p < 0.05), and lowered the lactate dehydrogenase (LDH) level (p < 0.05). Both at the phyla and genus levels, the relative abundance of Firmicutes and Streptococcus increased significantly, and the relative abundance of Acinetobacter decreased significantly when adding phytosterols (p < 0.05). Overall, phytosterols dietary supplementation promotes immunity and antioxidant capacity in finishing pigs and boosts growth performance by improving nutrient digestibility.
期刊：
JOURNAL OF ANIMAL BREEDING AND GENETICS,2025年142(2):223-236 ISSN：0931-2668
通讯作者：
Zhang, Z
作者机构：
[Wei, Chen; Gao, Yahui; Li, Jiaqi; Zhang, Zhe; Cai, Xiaodian; Zhang, Wenjing] South China Agr Univ, Coll Anim Sci, Natl Engn Res Ctr Breeding Swine Ind, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou, Peoples R China.;[Gao, Ning] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha, Peoples R China.;[Wu, Xibo; Si, Jinglei] Guangxi State Farmd Yongxin Anim Husb Grp Co Ltd, Nanning, Peoples R China.;[Yin, Tong] Justus Liebig Univ, Inst Anim Breeding & Genet, Giessen, Germany.;[Zhang, Z] South China Agr Univ, Dept Anim Breeding & Genet, Guangzhou 510642, Peoples R China.
通讯机构：
[Zhang, Z ] S;South China Agr Univ, Dept Anim Breeding & Genet, Guangzhou 510642, Peoples R China.
关键词：
combined population;genomic prediction;heritability enrichment;meta-analysis of genome-wide association studies
摘要：
The strategy of combining reference populations has been widely recognized as an effective way to enhance the accuracy of genomic prediction (GP). This study investigated the efficiency of genomic prediction using prior information and combined reference population. In total, prior information considering trait-associated single nucleotide polymorphisms (SNPs) obtained from meta-analysis of genome-wide association studies (GWAS meta-analysis) was incorporated into three models to assess the performance of GP using combined reference populations. Two different Yorkshire populations with imputed whole genome sequence (WGS) data (9,741,620 SNPs), named as P1 (1259 individuals) and P2 (1018 individuals), were used to predict genomic estimated breeding values for three live carcass traits, including backfat thickness, loin muscle area, and loin muscle depth. A 10 × 5 fold cross-validation was used to evaluate the prediction accuracy of 203 randomly selected candidate pigs from the P2 population and the reference population consisted of the remaining pigs from P2 and the stepwise added pigs from P1. By integrating SNPs with different p-value thresholds from GWAS meta-analysis downloaded from PigGTEx Project, the prediction accuracy of GBLUP, genomic feature BLUP (GFBLUP) and GBLUP given genetic architecture (BLUP|GA) were compared. Moreover, we explored effects of reference population size and heritability enrichment of genomic features on the prediction accuracy improvement of GFBLUP and BLUP|GA relative to GBLUP. The prediction accuracy of GBLUP using all WGS markers showed average improvement of 4.380% using the P1 + P2 reference population compared with the P2 reference population. Using the combined reference population, GFBLUP and BLUP|GA yielded 6.179% and 5.525% higher accuracies than GBLUP using all SNPs based on the single reference population, respectively. Positive regression coefficients were estimated in relation to the improvement in prediction accuracy (between GFBLUP/BLUP|GA and GBLUP) and the size of the reference as well as the heritability enrichment of genomic features. Compared to the classic GBLUP model, GFBLUP and BLUP|GA models integrating GWAS meta-analysis information increase the prediction accuracy and using combined populations with enlarged reference population size further enhances prediction accuracy of the two approaches. The heritability enrichment of genomic features can be used as an indicator to reflect weather prior information is accurately presented.
摘要：
This study evaluated the effects of tea residue combined with complex enzymes on the growth performance, serum biochemistry, meat quality, and intestinal microbiota of Xiangling fattening pigs. A total of 120 healthy Xiangling fattening pigs, with an average weight of 47.93 kg (±15.28 kg), were randomly assigned to five treatment groups, each comprising four replicates of six pigs. The control group (CON) received a basal diet; the experimental groups received a diet containing 5.8% fermented tea residue as an alternative energy and protein source (dry matter basis) and mixed additional 0 (CZ), 200 (M200), 400 (M400), and 800 (M800) complex enzymes. The trial lasted for 83 days. The results showed that tea residue and complex enzymes failed to affect growth performance (p > 0.05). Tea residue reduced the serum aspartate aminotransferase and alkaline phosphatase activities (p < 0.01), and complex enzyme supplementation lowered the total cholesterol levels and reduced the alanine aminotransferase activity (p < 0.05). Additionally, tea residue decreased the pH(24h) and b*(1h) values (p < 0.05), and complex enzyme supplementation increased the L*(24h) value and pork shear force (p < 0.05). In terms of amino acid content, tea residue significantly elevated aspartic acid and inosine monophosphate (p < 0.05), and complex enzyme addition increased the glutamic acid, lysine, alanine, valine, tyrosine, isoleucine, leucine, and phenylalanine levels (p < 0.05). The 800 mg/kg complex enzyme group exhibited a reduction in the C10:0, C15:0, and C17:0 contents (p < 0.05). Microbial analysis showed that tea residue promoted the abundance of Oribacterium and Butyricicoccus, while enzyme supplementation enriched Eggerthellaceae, Oscillospirales, and Peptococcaceae. Overall, the combination of tea residue and complex enzymes improved the pork quality, enhanced metabolic health markers, and modulated the gut microbiota composition, with the 400 mg/kg enzyme dose (M400) achieving the most pronounced benefits. These findings suggest a potential feeding strategy for improving pork quality without compromising growth performance.
作者机构：
[Linlin Yang] College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;[Minglang Cai; Lei Zhong; Yi Hu; Junzhi Zhang] College of Fisheries, Hunan Agricultural University, Changsha 410128, China;Laboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;[Yonghong Xie] Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;[Shouqi Xie] State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Chinese Academy of Sciences, Wuhan 430072, China
通讯机构：
[Yi Hu] C;College of Fisheries, Hunan Agricultural University, Changsha 410128, China
摘要：
The impacts of substituting dietary soybean meal (SBM) with yellow mealworm meal (YMM) were investigated during a 56-day feeding trial on growth, antioxidant capacity, immunity, intestinal morphology, and intestinal microbiota of grass carp. A total of 750 grass carp were divided into 5 groups (3 replications per group and 50 fish per replication) with different levels of YMM: SBM (control group), H25, H50, H75, and H100, for 8 weeks. The results showed that dietary YMM significantly increased final body weight (FW), weight gain (WG), and protein efficiency ratio (PER) in H25 group ( P < 0.05), however, complete substitution showed the opposite trend ( P < 0.05 for FW and WG). The liver antioxidant capacity was improved, manifested by enhanced superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) activities, glutathione (GSH) content and up-regulated antioxidant-related genes mediated by the Keap1-Nrf2 signaling pathway in the H25 group ( P < 0.05). However, in the H100 group oxidative stress occurred in parallel with impairment of hepatic function. Intestinal inflammation was aggravated in the H100 group as evidenced by the up-regulated pro-inflammatory gene expression mediated by the NF-κB pathway ( P < 0.05). Additionally, the activity of intestinal digestive enzymes for the grass carp was significantly reduced and accompanied with intestinal mucosal barrier dysfunction in the H100 group ( P < 0.05). In summary, replacement of SBM with 25% YMM showed positive influences on growth, antioxidant capacity, immunity, and intestinal health. Conversely, complete replacement of SBM with YMM triggered oxidative stress, caused liver function disorder, and impaired intestinal health in grass carp.
The impacts of substituting dietary soybean meal (SBM) with yellow mealworm meal (YMM) were investigated during a 56-day feeding trial on growth, antioxidant capacity, immunity, intestinal morphology, and intestinal microbiota of grass carp. A total of 750 grass carp were divided into 5 groups (3 replications per group and 50 fish per replication) with different levels of YMM: SBM (control group), H25, H50, H75, and H100, for 8 weeks. The results showed that dietary YMM significantly increased final body weight (FW), weight gain (WG), and protein efficiency ratio (PER) in H25 group ( P < 0.05), however, complete substitution showed the opposite trend ( P < 0.05 for FW and WG). The liver antioxidant capacity was improved, manifested by enhanced superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) activities, glutathione (GSH) content and up-regulated antioxidant-related genes mediated by the Keap1-Nrf2 signaling pathway in the H25 group ( P < 0.05). However, in the H100 group oxidative stress occurred in parallel with impairment of hepatic function. Intestinal inflammation was aggravated in the H100 group as evidenced by the up-regulated pro-inflammatory gene expression mediated by the NF-κB pathway ( P < 0.05). Additionally, the activity of intestinal digestive enzymes for the grass carp was significantly reduced and accompanied with intestinal mucosal barrier dysfunction in the H100 group ( P < 0.05). In summary, replacement of SBM with 25% YMM showed positive influences on growth, antioxidant capacity, immunity, and intestinal health. Conversely, complete replacement of SBM with YMM triggered oxidative stress, caused liver function disorder, and impaired intestinal health in grass carp.
通讯机构：
[Tan, B ] H;[Yin, YL ] C;[Yin, YL] Y;Hunan Agr Univ, Coll Anim Sci & Technol, Hunan Prov Key Lab Prod Qual Regulat Livestock & P, Changsha 410128, Hunan, Peoples R China.;Yuelushan Lab, Changsha 410128, Hunan, Peoples R China.
摘要：
BackgroundGut microbiota has been extensively demonstrated to modulate host lipid metabolism. Higher intramuscular fat (IMF) accumulation in Chinese indigenous breed pigs is associated with their special gut microbiota structure. However, the specific microbes and metabolic pathways responsible for lipid deposition are still poorly understood.ResultsIn the present study, a comparative analysis of the gut microbiota and metabolome in obese Ningxiang (NX) pigs and lean Duroc x Landrace x Yorkshire (DLY) pigs was conducted. The results revealed a higher abundance of gut lactobacilli and a correlation of branched-chain amino acid (BCAA) metabolism pathway in NX pigs. We proceeded to verify the roles of various lactobacilli strains originating from NX pigs in BCAA metabolism and lipids deposition in SD rats. We demonstrated that L. reuteri is a fundamental species responsible for modulating lipid deposition in NX pigs and that increased circulating levels of BCAA are positively linked to greater lipid deposition. Additionally, it has been verified that L. reuteri originating from NX pigs has the ability to improve BCAA synthesis in the gut and enhance IMF content in lean DLY pigs. The expression of genes related to lipid synthesis was also significantly upregulated.ConclusionsTaken together, our results imply that NX pig-derived L. reuteri regulates BCAA metabolism and plays a potential role in improving the meat quality of lean pig breeds through modulation of host intramuscular lipid deposition. The results provide a new strategy for improving the meat quality of commercial pigs by influencing host metabolism through supplementing dietary additives.F932jm79FQCSwcUD6yfj_aVideo AbstractConclusionsTaken together, our results imply that NX pig-derived L. reuteri regulates BCAA metabolism and plays a potential role in improving the meat quality of lean pig breeds through modulation of host intramuscular lipid deposition. The results provide a new strategy for improving the meat quality of commercial pigs by influencing host metabolism through supplementing dietary additives.F932jm79FQCSwcUD6yfj_aVideo Abstract
摘要：
Spirulina ( Arthrospira ) has been extensively applied in CO 2 biofixation, wastewater purification, and value-added bioproducts preparation. Light availability plays a pivotal role in Spirulina photoautotrophic cultivation, which is primary determined by characteristics of incident light and distribution of light within photobioreactors (PBRs). To clarify the role of light in Spirulina photoautotrophic cultivation, this review first analyzes the processes of light delivery and conversion in suspended PBRs. Then, effects of key light characteristics, including light intensity, spectrum, and photoperiod, on Spirulina growth and intracellular biochemical components synthesis are comprehensively summarized. Recent advancements in innovative PBR designs aimed at enhancing light utilization efficiency and promoting Spirulina growth are also highlighted. Finally, potential future research directions in the field of Spirulina photoautotrophic cultivation are outlined. Overall, this work provides a theoretical foundation and technical guidance for improving Spirulina production and specific target products synthesis from prespectives of light conditions regulation and PBRs design.
Spirulina ( Arthrospira ) has been extensively applied in CO 2 biofixation, wastewater purification, and value-added bioproducts preparation. Light availability plays a pivotal role in Spirulina photoautotrophic cultivation, which is primary determined by characteristics of incident light and distribution of light within photobioreactors (PBRs). To clarify the role of light in Spirulina photoautotrophic cultivation, this review first analyzes the processes of light delivery and conversion in suspended PBRs. Then, effects of key light characteristics, including light intensity, spectrum, and photoperiod, on Spirulina growth and intracellular biochemical components synthesis are comprehensively summarized. Recent advancements in innovative PBR designs aimed at enhancing light utilization efficiency and promoting Spirulina growth are also highlighted. Finally, potential future research directions in the field of Spirulina photoautotrophic cultivation are outlined. Overall, this work provides a theoretical foundation and technical guidance for improving Spirulina production and specific target products synthesis from prespectives of light conditions regulation and PBRs design.
摘要：
This study explored the optimal dietary available phosphorus (P) requirement for juvenile Asian red-tailed catfish ( Hemibagrus wyckioides ). Five isonitrogenous (42.00 %) and isolipidic (9.00 %) diets with monocalcium phosphate supplementation levels of 0.00 %, 0.75 %, 1.50 %, 2.25 %, and 3.00 % (namely P0, P0.75, P1.5, P2.25, and P3) were fed to fish for 56 d. Weight gain rate increased with phosphorus supplementation up to 2.25 % and then declined. The apparent digestibility coefficients (ADC) of phosphorus and calcium, as well as the phosphorus and calcium contents in the whole body and vertebrae, exhibited a positive correlation with dietary phosphorus levels, while the expression of intestinal genes related to phosphorus transport and metabolism showed a negative correlation with increasing dietary phosphorus levels. Using quadratic regression, the optimal phosphorus requirement was estimated at 0.87 % based on weight gain and 0.99 % based on vertebrae phosphorus content. Whole body crude lipid content and serum total cholesterol was significantly lower in P1.5, P2.25, and P3 compared to the P0 and P0.75 group ( P < 0.05 ). Liver lipid droplet volume and number were markedly reduced in P2.25 compared to the P0 group. Phosphorus deficiency resulted in stunted growth, reduced bone mineralization, and increased lipid accumulation in whole-body tissue and the liver. These findings provide practical guidance for phosphorus supplementation in H . wyckioides aquaculture, optimizing growth and bone mineralization while minimizing excess phosphorus in feeds.
This study explored the optimal dietary available phosphorus (P) requirement for juvenile Asian red-tailed catfish ( Hemibagrus wyckioides ). Five isonitrogenous (42.00 %) and isolipidic (9.00 %) diets with monocalcium phosphate supplementation levels of 0.00 %, 0.75 %, 1.50 %, 2.25 %, and 3.00 % (namely P0, P0.75, P1.5, P2.25, and P3) were fed to fish for 56 d. Weight gain rate increased with phosphorus supplementation up to 2.25 % and then declined. The apparent digestibility coefficients (ADC) of phosphorus and calcium, as well as the phosphorus and calcium contents in the whole body and vertebrae, exhibited a positive correlation with dietary phosphorus levels, while the expression of intestinal genes related to phosphorus transport and metabolism showed a negative correlation with increasing dietary phosphorus levels. Using quadratic regression, the optimal phosphorus requirement was estimated at 0.87 % based on weight gain and 0.99 % based on vertebrae phosphorus content. Whole body crude lipid content and serum total cholesterol was significantly lower in P1.5, P2.25, and P3 compared to the P0 and P0.75 group ( P < 0.05 ). Liver lipid droplet volume and number were markedly reduced in P2.25 compared to the P0 group. Phosphorus deficiency resulted in stunted growth, reduced bone mineralization, and increased lipid accumulation in whole-body tissue and the liver. These findings provide practical guidance for phosphorus supplementation in H . wyckioides aquaculture, optimizing growth and bone mineralization while minimizing excess phosphorus in feeds.
关键词：
digestibility;enzymatic hydrolysate of cottonseed protein;growth performance;metabolite;weaned piglets
摘要：
This experiment was conducted to investigate the effects of enzymatic hydrolysate of cottonseed protein (EHCP) replacing soybean meal on growth performance, nutrient digestibility, blood indexes and fecal volatile fatty acid concentrations of weaned piglets. Thirty 'Duroc × Landrace × Yorkshire' 28-day-old weaned piglets (male) with an initial body weight of 8.29 ± 0.76 kg were randomly divided into 3 treatment groups with 10 replicates per treatment and 1 pig per replicate. The control group was fed a corn-soybean meal basal diet, and the experimental groups were fed the basal diet supplemented with 2% 65%EHCP and 1.3% 85%EHCP to replace soybean meal, respectively. The experiment period was 21 days. Compared with the control group, the final body weight, average daily gain and average daily feed intake in 65%EHCP group and 85%EHCP group were significantly increased (p < 0.05); both the 65%EHCP and 85%EHCP groups exhibited significantly higher digestibility of energy and crude fat compared to the control group (p < 0.05). Notably, the energy digestibility in the 85%EHCP group was also significantly greater than that in the 65%EHCP group (p < 0.05). When compared to the control group, the blood urea nitrogen concentration was significantly lower in both the 65%EHCP and the 85%EHCP groups (p < 0.01). The concentrations of alanine, aspartic acid, glutamic acid, and tyrosine in serum were significantly higher in the 65%EHCP and 85%EHCP groups compared to the control group (p < 0.05). Additionally, in the 85%EHCP group, the concentrations of methionine and threonine in serum were significantly increased when compared to both the control group and the 65%EHCP group (p < 0.05). On the other hand, valine levels in the 65%EHCP group were significantly higher than in the 85%EHCP group (p < 0.05). Compared with the control group, fecal acetic acid, propionic acid, butyric acid and total volatile fatty acid concentration in the 65%EHCP group and 85%EHCP group were significantly increased (p < 0.01). In conclusion, dietary supplementation of EHCP can improve growth performance, nutrient digestibility, blood indexes and volatile fatty acids concentrations in feces of weaned piglets. This study suggests that EHCP can effectively substitute common soybean meal in the diets of weaned piglets and serves as a theoretical basis for its use in pig feeding practices.
期刊：
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING,2025年13(3):116576 ISSN：2213-2929
通讯作者：
Sun, YH
作者机构：
[Ma, Guoyu; Sun, Yahui; Yuan, Lu; Xiao, Dongdong; Wang, Liuqing] Nanjing Normal Univ, Sch Energy & Mech Engn, Nanjing 210023, Peoples R China.;[Cai, Zhongzhen; Zhang, Liang; Chen, Yu; Gao, Qiping] Tongwei Agr Dev Co Ltd, Tongwei Res Inst, Chengdu 610093, Peoples R China.;[Wu, Shusong] Hunan Agr Univ, Coll Anim Sci & Technol, Yuelushan Lab, Changsha 410128, Peoples R China.
通讯机构：
[Sun, YH ] N;Nanjing Normal Univ, Sch Energy & Mech Engn, Nanjing 210023, Peoples R China.
关键词：
Biomass;Photobioreactor;Spirulina platensis;LED light bar;Light distribution;Electric energy conversion efficiency
摘要：
Through photosynthesis, microalgae-based CO 2 fixation technology can simultaneously realize CO 2 reduction, wastewater purification, and valuable biomass accumulation. Considering the inherent high fluctuations in radiation intensity and discontinuity of sunlight, the adoption of electric-powered artificial illumination is inevitable to ensure the sustainable and stable production of microalgae biomass. While, poor conversion efficiency of electric energy into microalgae biomass resulted from severe light attenuation within aqua-suspended photobioreactors (PBRs) has greatly hindered microalgae photoautotrophic cultivation. Herein, aiming at boosting Spirulina platensis growth and the conversion efficiency of electric energy to Spirulina platensis biomass, an innovative and easily-implement approach through LED light bars arrangement manipulation was proposed. By affixing multiple parallelly-connected LED light bars onto outer wall surfaces of column PBRs, minimized light energy losses and improved light distributions within PBRs were realized, which contributed to superior Spirulina platensis growth. In particular, under the identical total electric power input for LED of 4.8 W, a 100 % improvement on Spirulina platensis biomass production was observed in column PBR with four LED light bars than that of with one LED light bar. Moreover, the maximum conversion efficiency of electric energy into Spirulina platensis biomass reached 6.09 g kW −1 h −1 and future application prospect of the proposed approach in commercial large-scale PBRs was discussed. Altogether, this work may provide guidance on the design of high-efficiency microalgae cultivation systems.
Through photosynthesis, microalgae-based CO 2 fixation technology can simultaneously realize CO 2 reduction, wastewater purification, and valuable biomass accumulation. Considering the inherent high fluctuations in radiation intensity and discontinuity of sunlight, the adoption of electric-powered artificial illumination is inevitable to ensure the sustainable and stable production of microalgae biomass. While, poor conversion efficiency of electric energy into microalgae biomass resulted from severe light attenuation within aqua-suspended photobioreactors (PBRs) has greatly hindered microalgae photoautotrophic cultivation. Herein, aiming at boosting Spirulina platensis growth and the conversion efficiency of electric energy to Spirulina platensis biomass, an innovative and easily-implement approach through LED light bars arrangement manipulation was proposed. By affixing multiple parallelly-connected LED light bars onto outer wall surfaces of column PBRs, minimized light energy losses and improved light distributions within PBRs were realized, which contributed to superior Spirulina platensis growth. In particular, under the identical total electric power input for LED of 4.8 W, a 100 % improvement on Spirulina platensis biomass production was observed in column PBR with four LED light bars than that of with one LED light bar. Moreover, the maximum conversion efficiency of electric energy into Spirulina platensis biomass reached 6.09 g kW −1 h −1 and future application prospect of the proposed approach in commercial large-scale PBRs was discussed. Altogether, this work may provide guidance on the design of high-efficiency microalgae cultivation systems.
关键词：
ATAC-seq;Ningxiang pigs;RNA-seq;chromatin accessibility;muscle development
摘要：
Meat production traits in pigs are critical economic characteristics, primarily influenced by the formation and development of skeletal muscle. Skeletal muscle development is regulated by a complex transcriptional network, which partly relies on chromatin accessibility for initiation. Ningxiang pigs, a renowned Chinese indigenous breed, are highly valued for their tender meat. However, studies focusing on skeletal muscle development in Ningxiang pigs, particularly from the perspective of chromatin accessibility, have not yet been reported. Based on this, the present study selected several key time points in the skeletal muscle development of Ningxiang pigs to perform Transposase-Accessible Chromatin Sequencing (ATAC-seq) and RNA sequencing (RNA-seq). This was carried out to identify key open chromatin regions and genes during different growth stages, which could influence skeletal muscle development in Ningxiang pigs. We collected longissimus dorsi muscle samples at postnatal days 14 (D14), 28 (D28), 85 (D85), 165 (D165), and 250 (D250). For each age, three individuals were collected for ATAC-seq and RNA-seq. After initial differential analysis among different ages, we identified 6412 differentially accessible chromatin peaks and 1464 differentially expressed genes. To clarify the key candidate transcription factors affecting the development of skeletal muscle in Ningxiang pigs, motif analysis of differential peaks revealed potential cis-regulatory elements with binding sites for transcription factors, including Fosl2 and JunB. Correlation analysis identified 56 overlapping genes and a significant positive correlation (r = 0.73, p = 1 × 10(-14)) between gene expression and chromatin accessibility. Key candidate genes such as HOXA10, closely related to skeletal muscle development, were specifically examined. These results enhance our understanding of the genetic and epigenetic regulatory mechanisms of porcine skeletal muscle development, providing a robust foundation for future molecular studies.
摘要：
Eimeria tenella (E. tenella) infection is a major cause of coccidiosis in chickens, leading to significant economic losses in the poultry industry due to its impact on the cecum. This study presents a comprehensive single-cell atlas of the chicken cecal epithelium by generating 7,394 cells using 10X Genomics single-cell RNA sequencing (scRNA-seq). We identified 13 distinct cell types, including key immune and epithelial populations, and characterized their gene expression profiles and cell-cell communication networks. Integration of this single-cell data with bulk RNA-seq data from E. tenella-infected chickens revealed significant alterations in cell type composition and state, particularly a marked decrease in APOB(+) enterocytes and an increase in cycling T cells during infection. Trajectory analysis of APOB(+) enterocytes uncovered shifts toward cellular states associated with cell death and a reduction in those linked to mitochondrial and cytoplasmic protection when infected with E. tenella. These findings highlight the substantial impact of E. tenella on epithelial integrity and immune responses, emphasizing the parasite's role in disrupting nutrient absorption and energy metabolism. Our single-cell atlas serves as a critical resource for understanding the cellular architecture of the chicken cecum and provides a valuable framework for future investigations into cecal diseases and metabolic functions, with potential applications in enhancing poultry health and productivity.
作者机构：
[Liu, Zhi-Wei; Tang, Pan-Pan; Tan, Yi-Cheng; Liu, Tian-Le] College of Food Science and Technology, Hunan Agricultural University, Changsha, 410128, China;[Liu, Xiu-Bin] College of Animal Science and Technology, Hunan Agricultural University, Changsha, 410128, China;[Liu, Chang; Cheng, Jun-Hu] School of Food Science and Engineering, South China University of Technology, Guangzhou, 510641, China;[Aadil, Rana Muhammad] National Institute of Food Science and Technology, University of Agriculture, Faisalabad, 38000, Pakistan
通讯机构：
[Zhi-Wei Liu] C;College of Food Science and Technology, Hunan Agricultural University, Changsha, 410128, China
摘要：
The potential of cold plasma (CP) treatment to promote the formation of amyloid fibrils (AFs) of ovalbumin (OVA) was evaluated relative to acidic heat fibrillation condition (pH = 2, 85 °C). Results indicated CP exhibited significant potential for promoting the formation of AFs of OVA, as evidenced by higher Thioflavin T (ThT) fluorescence intensity of AFs for CP-treated OVA (POVA) during the fibrillation process compared to AFs for native OVA under acidic heating conditions (NOVA). Long, curved and worm-like fibrils of CP-treated OVA with 2 min (POVA-2), which were thicker (3 nm in height) and longer (majority length ranging from 300 nm to 400 nm), were observed after 8 h of fibrillation, while irregular, short, worm-like fibrils of NOVA were detected. Unlike NOVA, which was only hydrolyzed into small peptides, the backbone of POVA-2 was cleaved into small peptide fragments, accompanied by the generation of dityrosine cross-linked aggregation/oligomer during fibrillation process, as evidenced by SDS-PAGE and dityrosine analysis. The result of hydrophobic, sulfhydryl and disulfide bonds, and dityrosine analysis showed hydrophobic interaction and dityrosine cross-links could be the main force driving the assembly and stacking of cross-β structures, leading to the formation of organized fibrillar structures for POVA-2, while only hydrophobic interaction was involved for NOVA. Additionally, analyses of emulsifying ability (EAI) and stability (ESI) of NOVA and POVA-2 displayed that both EAI and ESI of them were significantly improved, with POVA-2 exhibiting superior EAI compared to NOVA. Therefore, this study demonstrated CP is a promising technique to promote the generation of protein amyloid fibrils in a more efficient manner.
The potential of cold plasma (CP) treatment to promote the formation of amyloid fibrils (AFs) of ovalbumin (OVA) was evaluated relative to acidic heat fibrillation condition (pH = 2, 85 °C). Results indicated CP exhibited significant potential for promoting the formation of AFs of OVA, as evidenced by higher Thioflavin T (ThT) fluorescence intensity of AFs for CP-treated OVA (POVA) during the fibrillation process compared to AFs for native OVA under acidic heating conditions (NOVA). Long, curved and worm-like fibrils of CP-treated OVA with 2 min (POVA-2), which were thicker (3 nm in height) and longer (majority length ranging from 300 nm to 400 nm), were observed after 8 h of fibrillation, while irregular, short, worm-like fibrils of NOVA were detected. Unlike NOVA, which was only hydrolyzed into small peptides, the backbone of POVA-2 was cleaved into small peptide fragments, accompanied by the generation of dityrosine cross-linked aggregation/oligomer during fibrillation process, as evidenced by SDS-PAGE and dityrosine analysis. The result of hydrophobic, sulfhydryl and disulfide bonds, and dityrosine analysis showed hydrophobic interaction and dityrosine cross-links could be the main force driving the assembly and stacking of cross-β structures, leading to the formation of organized fibrillar structures for POVA-2, while only hydrophobic interaction was involved for NOVA. Additionally, analyses of emulsifying ability (EAI) and stability (ESI) of NOVA and POVA-2 displayed that both EAI and ESI of them were significantly improved, with POVA-2 exhibiting superior EAI compared to NOVA. Therefore, this study demonstrated CP is a promising technique to promote the generation of protein amyloid fibrils in a more efficient manner.
摘要：
The rising cost of fish meal (FM) in aquaculture feed has prompted the search for alternative protein sources like soybean meal (SBM). However, SBM-based diets can negatively affect fish growth, lipid metabolism, and intestinal health. One possible solution is to add cholesterol to SBM-based feeds to mitigate these adverse effects, but the optimal levels and the resulting benefits remain unclear. In this study, the impact of adding cholesterol to low FM and high soybean protein diets on the growth, lipid metabolism, and intestinal health of Monopterus albus was evaluated. Juvenile M. albus (initial body weight of 20.00 +/- 0.02 g) were fed a control diet with 42% FM + 22% SBM diet (FM group), 22% FM + 52% SBM diet (SBM group), and the SBM diet supplemented with 5 g/kg or 10 g/kg cholesterol (SBC5 and SBC10 groups, respectively). The results showed that the weight gain rate (WGR) and hepatosomatic index (HSI) were significantly lower in the SBM group compared to the FM group, but WGR increased with the addition of 10 g/kg cholesterol. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities increased significantly in the SBM group, indicating liver stress, but returned to normal levels in the SBC10 group. Cholesterol supplementation also improved serum and liver lipid profiles and significantly increased the contents of total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) in serum and high-density lipoprotein cholesterol (HDL-C) in the liver. In addition, cholesterol supplementation increased the activities of intestinal enzymes (e.g., amylase) and restored the structural integrity of the intestinal lining, including villus height and goblet cell count. Additionally, the SBC10 group exhibited a richer and more diverse intestinal microbiota, with increased abundance of Firmicutes and Bacteroidota. These findings demonstrate that supplementing 10 g/kg cholesterol in a high SBM diet improves growth performance, lipid metabolism, intestinal health, and gut microbiota composition in M. albus. This study provides a practical food-borne strategy to enhance the use of plant-based proteins in aquaculture while mitigating their negative effects, contributing to the sustainability of fish farming.
关键词：
advantages;aquaculture;new technologies and challenges;phage;resistance;therapy
摘要：
Aquaculture is essential for meeting future demands for food, yet it faces significant losses from infectious bacterial diseases. Aquaculture has recently been critically imperiled by the emergence of multi-drug-resistant bacteria, as it relies significantly on the use of antibiotics for prevention and treatment. The emergence of multidrug-resistant bacteria poses a critical threat to aquaculture, which heavily relies on antibiotics for prevention and treatment. Bacteriophage (phage) therapy has regained attention with the spread of drug-resistant bacteria. Phages are viruses that specifically infect bacteria and archaea. As a promising therapeutic strategy for aquatic bacterial diseases, phage therapy offers strong specificity, low resistance potential, rapid metabolism, ease of development, and cost-effectiveness. In this review, we discuss the advantages, opportunities, and challenges of phage therapy, summarizing the status of research and highlighting emerging technologies aimed at enhancing phage therapy in aquaculture. Finally, the review looks to the future, identifying scientific and technological advances necessary to establish phage therapy as a viable and universal alternative to antibiotics in aquaculture.
摘要：
The complement system, a critical component of innate immunity in fish, plays a pivotal role in the defense against Grass Carp Reovirus (GCRV) infection in grass carp. This study explores the structural characteristics of C1S , a crucial molecule in the classical pathway of the complement system, and its involvement in the response to GCRV infection. We found that the grass carp C1S gene comprises six domains similar to those in mammals: two CUB (Complement C1r/C1s, Uegf, Bmp1) domains, two CCP (Complement control protein) domains, one EGFCA (Calcium-binding epidermal growth factor) domain, and one Tryp_SPc (Trypsin‐like serine protease) domain, albeit without chromosomal collinearity to humans. Comparative analysis revealed that the identity and similarity of this gene with those in other species range from 30.6 to 89.4 % and 30.7–89.7 %, respectively. Phylogenetic analysis positioned C1S in close relation with R. klamathensis and D. rerio . Tissue expression profiles in both healthy and GCRV-infected grass carp indicated primary expression of C1S in the liver, with expression peaks at day 7 post-infection in the liver and spleen, and at day 5 in the kidney. Functional assays demonstrated that C1S activates the complement system via cleavage of complement component 3 (C3) into C3b, further inhibiting GCRV replication and upregulating antiviral genes IFN1 , IRF3 , and IRF7 . These findings elucidate the mechanism by which the complement system mediates resistance to GCRV infection in grass carp, offering a substantial theoretical foundation for further research.
The complement system, a critical component of innate immunity in fish, plays a pivotal role in the defense against Grass Carp Reovirus (GCRV) infection in grass carp. This study explores the structural characteristics of C1S , a crucial molecule in the classical pathway of the complement system, and its involvement in the response to GCRV infection. We found that the grass carp C1S gene comprises six domains similar to those in mammals: two CUB (Complement C1r/C1s, Uegf, Bmp1) domains, two CCP (Complement control protein) domains, one EGFCA (Calcium-binding epidermal growth factor) domain, and one Tryp_SPc (Trypsin‐like serine protease) domain, albeit without chromosomal collinearity to humans. Comparative analysis revealed that the identity and similarity of this gene with those in other species range from 30.6 to 89.4 % and 30.7–89.7 %, respectively. Phylogenetic analysis positioned C1S in close relation with R. klamathensis and D. rerio . Tissue expression profiles in both healthy and GCRV-infected grass carp indicated primary expression of C1S in the liver, with expression peaks at day 7 post-infection in the liver and spleen, and at day 5 in the kidney. Functional assays demonstrated that C1S activates the complement system via cleavage of complement component 3 (C3) into C3b, further inhibiting GCRV replication and upregulating antiviral genes IFN1 , IRF3 , and IRF7 . These findings elucidate the mechanism by which the complement system mediates resistance to GCRV infection in grass carp, offering a substantial theoretical foundation for further research.
摘要：
Machine learning (ML) methods have rapidly developed in various theoretical and practical research areas, including predicting genomic breeding values for large livestock animals. However, few studies have investigated the application of ML in broiler breeding. In this study, seven different ML methods—support vector regression (SVR), random forest (RF), gradient boosting decision tree (GBDT), extreme gradient boosting (XGBoost), light gradient boosting machine (LightGBM), kernel ridge regression (KRR) and multilayer perceptron (MLP) were employed to predict the genomic breeding values of laying traits, growth and carcass traits in a yellow-feathered broiler breeding population. The results indicated that classic methods, such as GBLUP and Bayesian, achieved superior prediction accuracy compared to ML methods in five of the eight traits. For half-eviscerated weight (HEW), ML methods showed an average improvement of 54.4% over GBLUP and Bayesian methods. Among the ML methods, SVR, RF, GBDT, and XGBoost exhibited improvements exceeding 60%, with respective values of 61.3%, 61.0%, 60.4%, and 60.7%; while MLP improved by 54.4% and LightGBM by 53.7%, KRR had the lowest improvement at 29.4%. For eviscerated weight (EW), ML methods still outperformed GBLUP and Bayesian methods. MLP gained the largest improvement at 19.0%, while SVR, RF, GBDT, XGBoost, LightGBM, and KRR improved by 15.0%, 16.5%, 9.5%, 7.0%, 1.6%, and 15.9%, respectively. Compared to default hyperparameters, the average improvement of ML methods with tuned hyperparameters was 34.0%, 32.9%, 27.0%, 19.3%, 26.8%, 13.2%, 18.9%, and 46.3%, respectively. The prediction accuracy of above algorithms could be optimized using genome-wide association study (GWAS) to select subsets of significant SNPs. This work provides valuable insights into genomic prediction, aiding genetic breeding in broilers.
Machine learning (ML) methods have rapidly developed in various theoretical and practical research areas, including predicting genomic breeding values for large livestock animals. However, few studies have investigated the application of ML in broiler breeding. In this study, seven different ML methods—support vector regression (SVR), random forest (RF), gradient boosting decision tree (GBDT), extreme gradient boosting (XGBoost), light gradient boosting machine (LightGBM), kernel ridge regression (KRR) and multilayer perceptron (MLP) were employed to predict the genomic breeding values of laying traits, growth and carcass traits in a yellow-feathered broiler breeding population. The results indicated that classic methods, such as GBLUP and Bayesian, achieved superior prediction accuracy compared to ML methods in five of the eight traits. For half-eviscerated weight (HEW), ML methods showed an average improvement of 54.4% over GBLUP and Bayesian methods. Among the ML methods, SVR, RF, GBDT, and XGBoost exhibited improvements exceeding 60%, with respective values of 61.3%, 61.0%, 60.4%, and 60.7%; while MLP improved by 54.4% and LightGBM by 53.7%, KRR had the lowest improvement at 29.4%. For eviscerated weight (EW), ML methods still outperformed GBLUP and Bayesian methods. MLP gained the largest improvement at 19.0%, while SVR, RF, GBDT, XGBoost, LightGBM, and KRR improved by 15.0%, 16.5%, 9.5%, 7.0%, 1.6%, and 15.9%, respectively. Compared to default hyperparameters, the average improvement of ML methods with tuned hyperparameters was 34.0%, 32.9%, 27.0%, 19.3%, 26.8%, 13.2%, 18.9%, and 46.3%, respectively. The prediction accuracy of above algorithms could be optimized using genome-wide association study (GWAS) to select subsets of significant SNPs. This work provides valuable insights into genomic prediction, aiding genetic breeding in broilers.
作者机构：
Authors to whom correspondence should be addressed.;[Yang, Xizi] Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha 410125, China;[Wu, Shusong; He, Jianhua] Authors to whom correspondence should be addressed.<&wdkj&>Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;[Hu, Ruizhi] National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha 410125, China<&wdkj&>Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China
通讯机构：
[Shusong Wu; Jianhua He] A;Authors to whom correspondence should be addressed.<&wdkj&>Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China
摘要：
This review systematically examines the influence of oxidative stress on the reproductive function of male livestock, with a particular focus on the modulation of autophagy. Spermatogenesis, a highly precise biological process, is vulnerable to a range of internal and external factors, among which oxidative stress notably disrupts autophagic processes within the testes. This disruption results in diminished sperm quality, impaired testosterone synthesis, and compromised integrity of the blood–testis barrier. Furthermore, this review elucidates the molecular mechanisms by which oxidative stress-induced autophagy dysfunction impairs spermatogenesis and mitochondrial function, consequently reducing sperm motility. These findings aim to provide a theoretical foundation and serve as a reference for improving reproductive performance and sperm quality in livestock.
