作者机构：
Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650500, China;[Yanna Ma; Zhiying Zhao; Liangyuan Peng; Shuchao Liao] Institute of Yunnan Circular Agricultural Industry, Pu’er 665000, China;[Haiming Ma] College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;Yunnan Province Key Laboratory for Porcine Gene Editing and Xenotransplantation, Yunnan Agricultural University, Kunming 650500, China;Author to whom correspondence should be addressed.
通讯机构：
[Hongjiang Wei] F;Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650500, China<&wdkj&>Yunnan Province Key Laboratory for Porcine Gene Editing and Xenotransplantation, Yunnan Agricultural University, Kunming 650500, China<&wdkj&>Author to whom correspondence should be addressed.
摘要：
The spleen is essential for immunity, mediating host defense against pathogens and regulating immunological homeostasis. Western-style diets commonly cause the aggregation of body fat and the emergence of obesity. This state might lead to damage to the spleen’s functions. However, the effects of Western-style diets on gene expression and metabolic regulation in the spleen have not yet been fully explored. In this study, C57BL/6 mice were fed either a high-fat diet (HFD) or standard chow (CHFD) for 10 weeks starting at 8 weeks old. Weekly weights were recorded, and spleens were weighed at 18 weeks. The results showed that HFD mice had significantly higher body weights from 12 weeks (p < 0.05) and a higher splenic index at 18 weeks (p < 0.01). HE staining revealed disrupted spleen structures and infarcted areas in the HFD group. Transcriptome sequencing highlighted immune-related pathways, including inflammatory response and interleukin-6 production. Among the differentially expressed genes (DEGs), PCK1, ALDH9A1, and ALDH7A1 were significantly upregulated in the HFD group, whereas PLA2G2F and PLA2G4F exhibited significant downregulation. APOB emerged as a key hub gene in PPI analysis. Metabolomics analysis identified significantly different metabolites (SDMs), including Rifamycins, 7-Ketodeoxycholic Acid, Folinic Acid, and Lotaustralin, as key biomarkers for an HFD, while 1-Methylnicotinamide and Prostaglandin E1 were significant for CHFD. KEGG enrichment linked glycerophospholipid and arachidonic acid metabolism to both transcriptome and metabolome results. The joint analysis of transcriptome and metabolome data revealed that SLC22A8 was negatively correlated with Biliverdin and 1-methylnicotinamide, and MCPT1 was inversely correlated with 7-Ketodeoxycholic Acid. These findings offer insights into the molecular mechanisms and metabolites that influence spleen immunity and systemic immune homeostasis.
作者机构：
These authors contributed equally to this work.;Hunan Provincial Key Laboratory for the Products Quality Regulation of Livestock and Poultry, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;Yuelushan Laboratory, Changsha 410128, China;Faculty of Agriculture, Kagoshima University, Korimoto, Kagoshima 8900065, Japan;Authors to whom correspondence should be addressed.
通讯机构：
[Chen Zhang; Xiongzhuo Tang] A;Authors to whom correspondence should be addressed.<&wdkj&>Hunan Provincial Key Laboratory for the Products Quality Regulation of Livestock and Poultry, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Yuelushan Laboratory, Changsha 410128, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>Hunan Provincial Key Laboratory for the Products Quality Regulation of Livestock and Poultry, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Institute of Yunnan Circular Agricultural Industry, Pu’er 665000, China<&wdkj&>Anhui Provincial Key Laboratory of Livestock and Poultry Product Safety, Institute of Animal Husbandry and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Hefei 230011, China
摘要：
The probiotic bacteria Lactobacillus fermentum ZC529 (L.f ZC529) has been identified from the colon of the Diannan small-ear (DSE) pig, but its intestinal protective function still lacks investigation. Here, we established a dextran sodium sulfate (DSS)-induced intestinal oxidative stress model in both Drosophila and porcine small intestinal epithelial (IPEC-J2) cell lines to explore the anti-oxidative and anti-inflammatory effects of L.f ZC529. The data showed that the intestinal colonization of L.f ZC529 counteracted DSS-induced intestinal oxidative stress and excessive reactive oxygen species (ROS) generation by activation of the CncC pathway, a homology of the nuclear factor erythroid 2-related factor 2 (Nrf2) in mammalian systems. Moreover, L.f ZC529 supplementation prevented flies from DSS-induced intestinal barrier damage, inflammation, abnormal excretory function, and shortened lifespan. Finally, L.f ZC529 also attenuated DSS-induced intestinal injury in the IPEC-J2 cell line by activating the Keap1-Nrf2 signaling and inhibiting the NF-κB signaling pathways. Together, this study unraveled the profound intestinal protective function of L.f ZC529 and provides its potential application as a new antioxidant in improving animal intestinal health as well as in developing a new probiotic in the food industry.
摘要：
This study aimed to investigate the effects of dietary isatidis root polysaccharide (IRP) on diarrhea, immunity, and intestinal health in weanling piglets. Forty healthy piglets were randomly assigned to five groups receiving varying dosages of IRP. The findings indicated that different concentrations of IRP significantly reduced diarrhea scores (p < 0.01). Notably, the serum levels of immunoglobulin A and immunoglobulin G increased linearly and quadratically (p < 0.01), while immunoglobulin M also showed a linear increase (p < 0.05) in IRP-fed piglets. The secretory immunoglobulin A levels in ileal contents were significantly higher compared to control piglets (p < 0.01). Key intestinal health parameters, including villus height, villus height-to-crypt depth ratio, and goblet cell numbers, showed linear and quadratic increases in both the jejunum and ileum (p < 0.05), while crypt depth decreased significantly (p < 0.01). Additionally, the expression of IL-10, ZO-1, occludin, and mucin2 was upregulated linearly and quadratically in IRP-fed piglets (p < 0.05). In cultured IPEC-J2 cells, ZO-1 and occludin expression levels significantly increased upon exposure to 400 μg/mL IRP (p < 0.01). Furthermore, the relative abundances of Escherichia coli, Ralstonia pickettii, and Desulfovibrio fairfieldensis decreased linearly with increasing dietary IRP concentration. In conclusion, IRP shows promise as an effective dietary supplement for mitigating diarrhea and enhancing intestinal health in early weaned piglets.
通讯机构：
[Deliang Li] H;Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization, College of Animal Science and Technology, Hunan Agriculture University, Changsha, China
摘要：
The rising cost of fish meal (FM) in aquaculture feed has prompted the search for alternative protein sources like soybean meal (SBM). However, SBM-based diets can negatively affect fish growth, lipid metabolism, and intestinal health. One possible solution is to add cholesterol to SBM-based feeds to mitigate these adverse effects, but the optimal levels and the resulting benefits remain unclear. In this study, the impact of adding cholesterol to low FM and high soybean protein diets on the growth, lipid metabolism, and intestinal health of Monopterus albus was evaluated. Juvenile M. albus (initial body weight of 20.00 ± 0.02 g) were fed a control diet with 42% FM + 22% SBM diet (FM group), 22% FM + 52% SBM diet (SBM group), and the SBM diet supplemented with 5 g/kg or 10 g/kg cholesterol (SBC5 and SBC10 groups, respectively). The results showed that the weight gain rate (WGR) and hepatosomatic index (HSI) were significantly lower in the SBM group compared to the FM group, but WGR increased with the addition of 10 g/kg cholesterol. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities increased significantly in the SBM group, indicating liver stress, but returned to normal levels in the SBC10 group. Cholesterol supplementation also improved serum and liver lipid profiles and significantly increased the contents of total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) in serum and high-density lipoprotein cholesterol (HDL-C) in the liver. In addition, cholesterol supplementation increased the activities of intestinal enzymes (e.g., amylase) and restored the structural integrity of the intestinal lining, including villus height and goblet cell count. Additionally, the SBC10 group exhibited a richer and more diverse intestinal microbiota, with increased abundance of Firmicutes and Bacteroidota. These findings demonstrate that supplementing 10 g/kg cholesterol in a high SBM diet improves growth performance, lipid metabolism, intestinal health, and gut microbiota composition in M. albus. This study provides a practical food-borne strategy to enhance the use of plant-based proteins in aquaculture while mitigating their negative effects, contributing to the sustainability of fish farming.
通讯机构：
[Wu, MM ] H;Hunan Agr Univ, Coll Anim Sci & Technol, Anim Nutr Genome & Germplasm Innovat Res Ctr, Changsha 410128, Hunan, Peoples R China.
关键词：
High fat diet;Obesity;Gut microbiome;Colitis;Goblet cell
摘要：
Obesity and its associated comorbidities have emerged as a major public health crisis. Accumulating evidence indicates that a high fat diet (HFD) may promote intestinal injury. However, the roles of intestinal goblet cells and specific gut microbiota composition in colitis under obesity conditions remain unclear. In this study, we employed a HFD-induced obesity model combined with dextran sulfate sodium (DSS) to induce colitis in mice. Our results reveal that obesity exacerbates DSS-induced colitis in the colon, as evidenced by increased intestinal barrier injury. These changes are associated with impaired goblet cell maturation, reduced mucus production, and a disrupted microbiota composition, notably characterized by an enrichment of pro-inflammatory bacteria Escherichia-Shigella and Helicobacter. In vitro , combined HFD and DSS treatment suppresses mucus secretion and alters the metabolic profile of LS174T cells, particularly affecting amino acid metabolism. Collectively, our findings reveal that HFD-induced obesity aggravates colitis severity, involving disruption of goblet cell function and gut microbial homeostasis, highlighting the importance of dietary intervention in obese individuals to alleviate intestinal inflammation.
Obesity and its associated comorbidities have emerged as a major public health crisis. Accumulating evidence indicates that a high fat diet (HFD) may promote intestinal injury. However, the roles of intestinal goblet cells and specific gut microbiota composition in colitis under obesity conditions remain unclear. In this study, we employed a HFD-induced obesity model combined with dextran sulfate sodium (DSS) to induce colitis in mice. Our results reveal that obesity exacerbates DSS-induced colitis in the colon, as evidenced by increased intestinal barrier injury. These changes are associated with impaired goblet cell maturation, reduced mucus production, and a disrupted microbiota composition, notably characterized by an enrichment of pro-inflammatory bacteria Escherichia-Shigella and Helicobacter. In vitro , combined HFD and DSS treatment suppresses mucus secretion and alters the metabolic profile of LS174T cells, particularly affecting amino acid metabolism. Collectively, our findings reveal that HFD-induced obesity aggravates colitis severity, involving disruption of goblet cell function and gut microbial homeostasis, highlighting the importance of dietary intervention in obese individuals to alleviate intestinal inflammation.
通讯机构：
[Liu, M ; Huang, P ] H;[Huang, P] Y;Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Peoples R China.;Yuelushan Lab, Changsha 410000, Peoples R China.;Hunan Agr Univ, Coll Vet, Changsha 410128, Hunan, Peoples R China.
摘要：
The perinatal period (from late gestation to the neonatal stage) in ruminants is a critical phase for fetal organ maturation, where ecological succession of gastrointestinal microbial communities significantly impacts livestock production efficiency. However, research remains insufficient regarding the distribution patterns and functional annotation of microbial communities across different gastrointestinal compartments during this period. This study characterized early microbiota dynamics in Hutianshi Goats using 16S rRNA sequencing (4 fetal goats at 90 ± 10 gestational days) and metagenomics (3 7-day-old goatkids). The fetal goat group generated 852,694 valid reads, yielding 688,277 high-quality reads after chimera removal for downstream analysis. The 7-day-old goat kids group produced 1,081,588,182 final valid reads, after data processing and assembly, 8,561,345 contigs were generated. Gene prediction identified 6,095,352 genes. Multi-database annotations (NR, KEGG, CAZy, etc.) revealed functional potential and antimicrobial resistance traits. The public release of this dataset facilitates academic understanding of microbial community dynamics and host-microbe interactions during this developmental stage, providing both theoretical foundations and data resources for ruminant developmental biology and precision breeding regulation.
摘要：
This study explored the optimal dietary available phosphorus (P) requirement for juvenile Asian red-tailed catfish ( Hemibagrus wyckioides ). Five isonitrogenous (42.00 %) and isolipidic (9.00 %) diets with monocalcium phosphate supplementation levels of 0.00 %, 0.75 %, 1.50 %, 2.25 %, and 3.00 % (namely P0, P0.75, P1.5, P2.25, and P3) were fed to fish for 56 d. Weight gain rate increased with phosphorus supplementation up to 2.25 % and then declined. The apparent digestibility coefficients (ADC) of phosphorus and calcium, as well as the phosphorus and calcium contents in the whole body and vertebrae, exhibited a positive correlation with dietary phosphorus levels, while the expression of intestinal genes related to phosphorus transport and metabolism showed a negative correlation with increasing dietary phosphorus levels. Using quadratic regression, the optimal phosphorus requirement was estimated at 0.87 % based on weight gain and 0.99 % based on vertebrae phosphorus content. Whole body crude lipid content and serum total cholesterol was significantly lower in P1.5, P2.25, and P3 compared to the P0 and P0.75 group ( P < 0.05 ). Liver lipid droplet volume and number were markedly reduced in P2.25 compared to the P0 group. Phosphorus deficiency resulted in stunted growth, reduced bone mineralization, and increased lipid accumulation in whole-body tissue and the liver. These findings provide practical guidance for phosphorus supplementation in H . wyckioides aquaculture, optimizing growth and bone mineralization while minimizing excess phosphorus in feeds.
This study explored the optimal dietary available phosphorus (P) requirement for juvenile Asian red-tailed catfish ( Hemibagrus wyckioides ). Five isonitrogenous (42.00 %) and isolipidic (9.00 %) diets with monocalcium phosphate supplementation levels of 0.00 %, 0.75 %, 1.50 %, 2.25 %, and 3.00 % (namely P0, P0.75, P1.5, P2.25, and P3) were fed to fish for 56 d. Weight gain rate increased with phosphorus supplementation up to 2.25 % and then declined. The apparent digestibility coefficients (ADC) of phosphorus and calcium, as well as the phosphorus and calcium contents in the whole body and vertebrae, exhibited a positive correlation with dietary phosphorus levels, while the expression of intestinal genes related to phosphorus transport and metabolism showed a negative correlation with increasing dietary phosphorus levels. Using quadratic regression, the optimal phosphorus requirement was estimated at 0.87 % based on weight gain and 0.99 % based on vertebrae phosphorus content. Whole body crude lipid content and serum total cholesterol was significantly lower in P1.5, P2.25, and P3 compared to the P0 and P0.75 group ( P < 0.05 ). Liver lipid droplet volume and number were markedly reduced in P2.25 compared to the P0 group. Phosphorus deficiency resulted in stunted growth, reduced bone mineralization, and increased lipid accumulation in whole-body tissue and the liver. These findings provide practical guidance for phosphorus supplementation in H . wyckioides aquaculture, optimizing growth and bone mineralization while minimizing excess phosphorus in feeds.
摘要：
The complement system, a critical component of innate immunity in fish, plays a pivotal role in the defense against Grass Carp Reovirus (GCRV) infection in grass carp. This study explores the structural characteristics of C1S , a crucial molecule in the classical pathway of the complement system, and its involvement in the response to GCRV infection. We found that the grass carp C1S gene comprises six domains similar to those in mammals: two CUB (Complement C1r/C1s, Uegf, Bmp1) domains, two CCP (Complement control protein) domains, one EGFCA (Calcium-binding epidermal growth factor) domain, and one Tryp_SPc (Trypsin‐like serine protease) domain, albeit without chromosomal collinearity to humans. Comparative analysis revealed that the identity and similarity of this gene with those in other species range from 30.6 to 89.4 % and 30.7–89.7 %, respectively. Phylogenetic analysis positioned C1S in close relation with R. klamathensis and D. rerio . Tissue expression profiles in both healthy and GCRV-infected grass carp indicated primary expression of C1S in the liver, with expression peaks at day 7 post-infection in the liver and spleen, and at day 5 in the kidney. Functional assays demonstrated that C1S activates the complement system via cleavage of complement component 3 (C3) into C3b, further inhibiting GCRV replication and upregulating antiviral genes IFN1 , IRF3 , and IRF7 . These findings elucidate the mechanism by which the complement system mediates resistance to GCRV infection in grass carp, offering a substantial theoretical foundation for further research.
The complement system, a critical component of innate immunity in fish, plays a pivotal role in the defense against Grass Carp Reovirus (GCRV) infection in grass carp. This study explores the structural characteristics of C1S , a crucial molecule in the classical pathway of the complement system, and its involvement in the response to GCRV infection. We found that the grass carp C1S gene comprises six domains similar to those in mammals: two CUB (Complement C1r/C1s, Uegf, Bmp1) domains, two CCP (Complement control protein) domains, one EGFCA (Calcium-binding epidermal growth factor) domain, and one Tryp_SPc (Trypsin‐like serine protease) domain, albeit without chromosomal collinearity to humans. Comparative analysis revealed that the identity and similarity of this gene with those in other species range from 30.6 to 89.4 % and 30.7–89.7 %, respectively. Phylogenetic analysis positioned C1S in close relation with R. klamathensis and D. rerio . Tissue expression profiles in both healthy and GCRV-infected grass carp indicated primary expression of C1S in the liver, with expression peaks at day 7 post-infection in the liver and spleen, and at day 5 in the kidney. Functional assays demonstrated that C1S activates the complement system via cleavage of complement component 3 (C3) into C3b, further inhibiting GCRV replication and upregulating antiviral genes IFN1 , IRF3 , and IRF7 . These findings elucidate the mechanism by which the complement system mediates resistance to GCRV infection in grass carp, offering a substantial theoretical foundation for further research.
摘要：
The impacts of substituting dietary soybean meal (SBM) with yellow mealworm meal (YMM) were investigated during a 56-day feeding trial on growth, antioxidant capacity, immunity, intestinal morphology, and intestinal microbiota of grass carp. A total of 750 grass carp were divided into 5 groups (3 replications per group and 50 fish per replication) with different levels of YMM: SBM (control group), H25, H50, H75, and H100, for 8 weeks. The results showed that dietary YMM significantly increased final body weight (FW), weight gain (WG), and protein efficiency ratio (PER) in H25 group ( P < 0.05), however, complete substitution showed the opposite trend ( P < 0.05 for FW and WG). The liver antioxidant capacity was improved, manifested by enhanced superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) activities, glutathione (GSH) content and up-regulated antioxidant-related genes mediated by the Keap1-Nrf2 signaling pathway in the H25 group ( P < 0.05). However, in the H100 group oxidative stress occurred in parallel with impairment of hepatic function. Intestinal inflammation was aggravated in the H100 group as evidenced by the up-regulated pro-inflammatory gene expression mediated by the NF-κB pathway ( P < 0.05). Additionally, the activity of intestinal digestive enzymes for the grass carp was significantly reduced and accompanied with intestinal mucosal barrier dysfunction in the H100 group ( P < 0.05). In summary, replacement of SBM with 25% YMM showed positive influences on growth, antioxidant capacity, immunity, and intestinal health. Conversely, complete replacement of SBM with YMM triggered oxidative stress, caused liver function disorder, and impaired intestinal health in grass carp.
The impacts of substituting dietary soybean meal (SBM) with yellow mealworm meal (YMM) were investigated during a 56-day feeding trial on growth, antioxidant capacity, immunity, intestinal morphology, and intestinal microbiota of grass carp. A total of 750 grass carp were divided into 5 groups (3 replications per group and 50 fish per replication) with different levels of YMM: SBM (control group), H25, H50, H75, and H100, for 8 weeks. The results showed that dietary YMM significantly increased final body weight (FW), weight gain (WG), and protein efficiency ratio (PER) in H25 group ( P < 0.05), however, complete substitution showed the opposite trend ( P < 0.05 for FW and WG). The liver antioxidant capacity was improved, manifested by enhanced superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) activities, glutathione (GSH) content and up-regulated antioxidant-related genes mediated by the Keap1-Nrf2 signaling pathway in the H25 group ( P < 0.05). However, in the H100 group oxidative stress occurred in parallel with impairment of hepatic function. Intestinal inflammation was aggravated in the H100 group as evidenced by the up-regulated pro-inflammatory gene expression mediated by the NF-κB pathway ( P < 0.05). Additionally, the activity of intestinal digestive enzymes for the grass carp was significantly reduced and accompanied with intestinal mucosal barrier dysfunction in the H100 group ( P < 0.05). In summary, replacement of SBM with 25% YMM showed positive influences on growth, antioxidant capacity, immunity, and intestinal health. Conversely, complete replacement of SBM with YMM triggered oxidative stress, caused liver function disorder, and impaired intestinal health in grass carp.
摘要：
This study evaluated the effects of tea residue combined with complex enzymes on the growth performance, serum biochemistry, meat quality, and intestinal microbiota of Xiangling fattening pigs. A total of 120 healthy Xiangling fattening pigs, with an average weight of 47.93 kg (±15.28 kg), were randomly assigned to five treatment groups, each comprising four replicates of six pigs. The control group (CON) received a basal diet; the experimental groups received a diet containing 5.8% fermented tea residue as an alternative energy and protein source (dry matter basis) and mixed additional 0 (CZ), 200 (M200), 400 (M400), and 800 (M800) complex enzymes. The trial lasted for 83 days. The results showed that tea residue and complex enzymes failed to affect growth performance (p > 0.05). Tea residue reduced the serum aspartate aminotransferase and alkaline phosphatase activities (p < 0.01), and complex enzyme supplementation lowered the total cholesterol levels and reduced the alanine aminotransferase activity (p < 0.05). Additionally, tea residue decreased the pH(24h) and b*(1h) values (p < 0.05), and complex enzyme supplementation increased the L*(24h) value and pork shear force (p < 0.05). In terms of amino acid content, tea residue significantly elevated aspartic acid and inosine monophosphate (p < 0.05), and complex enzyme addition increased the glutamic acid, lysine, alanine, valine, tyrosine, isoleucine, leucine, and phenylalanine levels (p < 0.05). The 800 mg/kg complex enzyme group exhibited a reduction in the C10:0, C15:0, and C17:0 contents (p < 0.05). Microbial analysis showed that tea residue promoted the abundance of Oribacterium and Butyricicoccus, while enzyme supplementation enriched Eggerthellaceae, Oscillospirales, and Peptococcaceae. Overall, the combination of tea residue and complex enzymes improved the pork quality, enhanced metabolic health markers, and modulated the gut microbiota composition, with the 400 mg/kg enzyme dose (M400) achieving the most pronounced benefits. These findings suggest a potential feeding strategy for improving pork quality without compromising growth performance.
作者机构：
Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan 410125, China;[Chen, Jiashun] College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China;[Wan, Mengliao] Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan 410125, China<&wdkj&>College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;[Yin, Yulong; Duan, Yehui] Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan 410125, China<&wdkj&>College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China
通讯机构：
[Yehui Duan] H;Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan 410125, China<&wdkj&>College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China
摘要：
Methionine (Met) is the only sulfur-containing essential amino acid for animals and is the second or third limiting amino acid in swine diets. It plays a role not only in protein synthesis, but also as an important methyl donor to participate in various biochemical reactions. Additionally, Met can be converted into several functional derivatives, such as S-adenosylmethionine (SAM) and homocysteine (Hcy) through which it exerts its biological functions. Specifically, appropriate supplementation with Met or SAM has been shown to provide a wide range of health-promoting effects, particularly in enhancing reproductive performance, improving intestinal morphology, regulating hepatic lipid metabolism, promoting muscle growth, and improving meat quality. In contrast, elevated Hcy levels can increase oxidative stress and inflammation, negatively affecting reproductive performance. Supplemental Met has been widely used in swine production for many years. This review summarizes recent advances in the application of Met and its derivatives in the pig industry, and explores their mechanism of action and optimal dosage as a feed additive, aiming to support the effective development and rational use of Met.
Methionine (Met) is the only sulfur-containing essential amino acid for animals and is the second or third limiting amino acid in swine diets. It plays a role not only in protein synthesis, but also as an important methyl donor to participate in various biochemical reactions. Additionally, Met can be converted into several functional derivatives, such as S-adenosylmethionine (SAM) and homocysteine (Hcy) through which it exerts its biological functions. Specifically, appropriate supplementation with Met or SAM has been shown to provide a wide range of health-promoting effects, particularly in enhancing reproductive performance, improving intestinal morphology, regulating hepatic lipid metabolism, promoting muscle growth, and improving meat quality. In contrast, elevated Hcy levels can increase oxidative stress and inflammation, negatively affecting reproductive performance. Supplemental Met has been widely used in swine production for many years. This review summarizes recent advances in the application of Met and its derivatives in the pig industry, and explores their mechanism of action and optimal dosage as a feed additive, aiming to support the effective development and rational use of Met.
关键词：
digestibility;enzymatic hydrolysate of cottonseed protein;growth performance;metabolite;weaned piglets
摘要：
This experiment was conducted to investigate the effects of enzymatic hydrolysate of cottonseed protein (EHCP) replacing soybean meal on growth performance, nutrient digestibility, blood indexes and fecal volatile fatty acid concentrations of weaned piglets. Thirty 'Duroc × Landrace × Yorkshire' 28-day-old weaned piglets (male) with an initial body weight of 8.29 ± 0.76 kg were randomly divided into 3 treatment groups with 10 replicates per treatment and 1 pig per replicate. The control group was fed a corn-soybean meal basal diet, and the experimental groups were fed the basal diet supplemented with 2% 65%EHCP and 1.3% 85%EHCP to replace soybean meal, respectively. The experiment period was 21 days. Compared with the control group, the final body weight, average daily gain and average daily feed intake in 65%EHCP group and 85%EHCP group were significantly increased (p < 0.05); both the 65%EHCP and 85%EHCP groups exhibited significantly higher digestibility of energy and crude fat compared to the control group (p < 0.05). Notably, the energy digestibility in the 85%EHCP group was also significantly greater than that in the 65%EHCP group (p < 0.05). When compared to the control group, the blood urea nitrogen concentration was significantly lower in both the 65%EHCP and the 85%EHCP groups (p < 0.01). The concentrations of alanine, aspartic acid, glutamic acid, and tyrosine in serum were significantly higher in the 65%EHCP and 85%EHCP groups compared to the control group (p < 0.05). Additionally, in the 85%EHCP group, the concentrations of methionine and threonine in serum were significantly increased when compared to both the control group and the 65%EHCP group (p < 0.05). On the other hand, valine levels in the 65%EHCP group were significantly higher than in the 85%EHCP group (p < 0.05). Compared with the control group, fecal acetic acid, propionic acid, butyric acid and total volatile fatty acid concentration in the 65%EHCP group and 85%EHCP group were significantly increased (p < 0.01). In conclusion, dietary supplementation of EHCP can improve growth performance, nutrient digestibility, blood indexes and volatile fatty acids concentrations in feces of weaned piglets. This study suggests that EHCP can effectively substitute common soybean meal in the diets of weaned piglets and serves as a theoretical basis for its use in pig feeding practices.
摘要：
Intestinal diseases are highly prevalent, affecting millions worldwide and significantly contributing to global morbidity. The treatment of complex disorders, such as inflammatory bowel disease (IBD) and colorectal cancer (CRC), remains challenging due to multifactorial etiologies, diverse patient responses, and the limitations of current therapeutic strategies. Although the gut microbiota clearly plays a role in regulating the onset of intestinal diseases, few studies have explored the epigenetic factors by which the microbiota contributes to disease development. Here, the latest insights into the molecular mechanisms underlying the bidirectional influence between gut microbiota and epigenetic modifications are discussed, including DNA methylation, histone modifications, non-coding RNAs, and N6-methyladenosine (m(6)A). Importantly, mechanistic studies based on animal models or human cells have demonstrated that the gut microbiota, and other environmental factors, influence targeted gene expression and activate immune pathways through host epigenetic dysregulation, which are closely associated with the development of IBD and CRC. Furthermore, potential microbiome interventions, including probiotics, prebiotics and postbiotics, fecal microbiota transplantation (FMT), dietary modifications, and phage therapy, have been proposed as innovative therapeutic strategies to correct these abnormal epigenetic patterns associated with the diseases. Overall, addressing microbiome dysbiosis and its epigenetic consequences presents a promising frontier in the treatment of intestinal diseases, offering the potential to not only restore microbial balance but also provide more targeted and personalized therapeutic strategies for better patient outcomes.
摘要：
To investigate the effects of feeding frequency on the growth, intestinal health, and metabolism of larval red-tailed catfish (Hemibagrus wyckioides) cultured in land-based circular tanks, a 56-day feeding trial was conducted. A total of 450 fish (8.47 +/- 0.36 g) were randomly allocated to three feeding frequencies: twice (F2), three times (F3), and four times (F4) daily. The results revealed that the F3 group had a significantly better feed conversion rate, specific growth rate, and weight gain rate compared to the F4 group (p < 0.05). Lipase activity and villus height were also significantly greater in the F3 group compared to the other groups (p < 0.05). The transcriptome of the F3 group showed significant enrichment in immune- and metabolism-related pathways. Additionally, the F3 group had a higher abundance of beneficial Clostridium compared to the other groups. Plesiomonas was identified as the main contributor in the F3 group, and its abundance was significantly decreased in the F4 group (p < 0.05). These findings indicated that a feeding frequency of three times per day improves the growth performance of H. wyckioides in aquaculture by increasing the abundance of beneficial Clostridium and Plesiomonas, activating multiple immune pathways, and enhancing amino acid metabolism.
摘要：
The slow development of the Internet of Things (IoT) in pig production, due to the lack of high-quality data, limited large-scale models, and low hardware coverage, has hindered the widespread adoption of precision feeding practices. This study aimed to address these challenges by providing a standardized dataset as a foundation for IoT development and constructing predictive models focused on birth litter weight (BLW) and weaned litter weight (WLW). To achieve these objectives, two comprehensive datasets consisting of 10,089 sow characteristics were collected. By comparing eight different algorithms, GBDT algorithm was selected as the optimal algorithm for modeling of BLW and WLW. The datasets were divided into a 90 % sample for model derivation, with the remaining 10 % used for model validation. The models for both BLW and WLW datasets exhibited consistent performance between main and validation cohorts, with low error magnitudes and high relative accuracy (MAE: 1.8–2.5, MAPE: 2.55 %–18.41 %, R > 60 %), indicating robustness and generalizability to unseen data. Delving deeper, the SHAP summary plots illustrated that in the model for BLW, G.ADFIp2, G.ADFIp3, G.ADFIp4, G.ADF and parity had a significant impact on the prediction. In the WLW model, the key influencing factors were weaned litter size, duration of lactation, parity, and birth litter weight. SHAP force and dependence plots had uncovered intricate effects of various features on the model’s outcomes. To enhance accessibility, we developed a user-friendly visualization and prediction website using the Streamlit Python framework. These critical research findings provide decision-makers with invaluable insights, fostering advancements in precision feeding models and IoT technologies in the swine industry. Ultimately, this contributes to the overarching goal of enhancing the comprehensive sustainability of livestock farming.
The slow development of the Internet of Things (IoT) in pig production, due to the lack of high-quality data, limited large-scale models, and low hardware coverage, has hindered the widespread adoption of precision feeding practices. This study aimed to address these challenges by providing a standardized dataset as a foundation for IoT development and constructing predictive models focused on birth litter weight (BLW) and weaned litter weight (WLW). To achieve these objectives, two comprehensive datasets consisting of 10,089 sow characteristics were collected. By comparing eight different algorithms, GBDT algorithm was selected as the optimal algorithm for modeling of BLW and WLW. The datasets were divided into a 90 % sample for model derivation, with the remaining 10 % used for model validation. The models for both BLW and WLW datasets exhibited consistent performance between main and validation cohorts, with low error magnitudes and high relative accuracy (MAE: 1.8–2.5, MAPE: 2.55 %–18.41 %, R > 60 %), indicating robustness and generalizability to unseen data. Delving deeper, the SHAP summary plots illustrated that in the model for BLW, G.ADFIp2, G.ADFIp3, G.ADFIp4, G.ADF and parity had a significant impact on the prediction. In the WLW model, the key influencing factors were weaned litter size, duration of lactation, parity, and birth litter weight. SHAP force and dependence plots had uncovered intricate effects of various features on the model’s outcomes. To enhance accessibility, we developed a user-friendly visualization and prediction website using the Streamlit Python framework. These critical research findings provide decision-makers with invaluable insights, fostering advancements in precision feeding models and IoT technologies in the swine industry. Ultimately, this contributes to the overarching goal of enhancing the comprehensive sustainability of livestock farming.
作者机构：
Authors to whom correspondence should be addressed.;[Yang, Xizi] Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha 410125, China;[Wu, Shusong; He, Jianhua] Authors to whom correspondence should be addressed.<&wdkj&>Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;[Hu, Ruizhi] National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha 410125, China<&wdkj&>Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China
通讯机构：
[Shusong Wu; Jianhua He] A;Authors to whom correspondence should be addressed.<&wdkj&>Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China
摘要：
This review systematically examines the influence of oxidative stress on the reproductive function of male livestock, with a particular focus on the modulation of autophagy. Spermatogenesis, a highly precise biological process, is vulnerable to a range of internal and external factors, among which oxidative stress notably disrupts autophagic processes within the testes. This disruption results in diminished sperm quality, impaired testosterone synthesis, and compromised integrity of the blood–testis barrier. Furthermore, this review elucidates the molecular mechanisms by which oxidative stress-induced autophagy dysfunction impairs spermatogenesis and mitochondrial function, consequently reducing sperm motility. These findings aim to provide a theoretical foundation and serve as a reference for improving reproductive performance and sperm quality in livestock.
摘要：
This study investigated the effects of fermented ramie feed (FRF) on the growth performance, serum biochemistry, metabolic capacity, antioxidant capacity, and intestinal health of Linwu ducks. A total of 480 female Linwu ducks (age: 28 days) were randomly divided into five treatments (six replicates per group; 16 ducks per replicate). The control group received a basal diet and the treatment groups received the basal diet supplemented with by 3%, 6%, 9% or 12% FRF. The experiment lasted 21 days. Notably, 3% and 6% FRF trended to increase the final body weight (P = 0.097) and significantly increased the thymus index (P < 0.05). Regarding serum indices, FRF greatly reduced the triglyceride and glucose contents, and increased the catalase and glutathione peroxidase activities (P < 0.05). Besides, FRF significantly increased the apparent metabolic rates of dry matter, crude protein, crude ash and gross energy (P < 0.05). Furthermore, FRF remarkably improved villus height in the duodenum. FRF also increased the relative abundances of beneficial bacteria (Alistipes and Barnesiella) and reduced the relative abundances of harmful parasitic bacteria (Desulfovibrio and Enterenecus) in the cecum (P < 0.05). Thus, partial FRF substitution greatly improved serum biochemistry, antioxidant capacity, metabolic capacity and intestinal health in Linwu ducks, thereby enhancing the growth performance to a certain extent. The optimal level of FRF substitution is 3%-6% according to the impacts of growth performance and overall health.
通讯机构：
[Tan, B ] H;[Yin, YL ] C;[Yin, YL] Y;Hunan Agr Univ, Coll Anim Sci & Technol, Hunan Prov Key Lab Prod Qual Regulat Livestock & P, Changsha 410128, Hunan, Peoples R China.;Yuelushan Lab, Changsha 410128, Hunan, Peoples R China.
摘要：
BackgroundGut microbiota has been extensively demonstrated to modulate host lipid metabolism. Higher intramuscular fat (IMF) accumulation in Chinese indigenous breed pigs is associated with their special gut microbiota structure. However, the specific microbes and metabolic pathways responsible for lipid deposition are still poorly understood.ResultsIn the present study, a comparative analysis of the gut microbiota and metabolome in obese Ningxiang (NX) pigs and lean Duroc x Landrace x Yorkshire (DLY) pigs was conducted. The results revealed a higher abundance of gut lactobacilli and a correlation of branched-chain amino acid (BCAA) metabolism pathway in NX pigs. We proceeded to verify the roles of various lactobacilli strains originating from NX pigs in BCAA metabolism and lipids deposition in SD rats. We demonstrated that L. reuteri is a fundamental species responsible for modulating lipid deposition in NX pigs and that increased circulating levels of BCAA are positively linked to greater lipid deposition. Additionally, it has been verified that L. reuteri originating from NX pigs has the ability to improve BCAA synthesis in the gut and enhance IMF content in lean DLY pigs. The expression of genes related to lipid synthesis was also significantly upregulated.ConclusionsTaken together, our results imply that NX pig-derived L. reuteri regulates BCAA metabolism and plays a potential role in improving the meat quality of lean pig breeds through modulation of host intramuscular lipid deposition. The results provide a new strategy for improving the meat quality of commercial pigs by influencing host metabolism through supplementing dietary additives.F932jm79FQCSwcUD6yfj_aVideo AbstractConclusionsTaken together, our results imply that NX pig-derived L. reuteri regulates BCAA metabolism and plays a potential role in improving the meat quality of lean pig breeds through modulation of host intramuscular lipid deposition. The results provide a new strategy for improving the meat quality of commercial pigs by influencing host metabolism through supplementing dietary additives.F932jm79FQCSwcUD6yfj_aVideo Abstract
摘要：
Spirulina ( Arthrospira ) has been extensively applied in CO 2 biofixation, wastewater purification, and value-added bioproducts preparation. Light availability plays a pivotal role in Spirulina photoautotrophic cultivation, which is primary determined by characteristics of incident light and distribution of light within photobioreactors (PBRs). To clarify the role of light in Spirulina photoautotrophic cultivation, this review first analyzes the processes of light delivery and conversion in suspended PBRs. Then, effects of key light characteristics, including light intensity, spectrum, and photoperiod, on Spirulina growth and intracellular biochemical components synthesis are comprehensively summarized. Recent advancements in innovative PBR designs aimed at enhancing light utilization efficiency and promoting Spirulina growth are also highlighted. Finally, potential future research directions in the field of Spirulina photoautotrophic cultivation are outlined. Overall, this work provides a theoretical foundation and technical guidance for improving Spirulina production and specific target products synthesis from prespectives of light conditions regulation and PBRs design.
Spirulina ( Arthrospira ) has been extensively applied in CO 2 biofixation, wastewater purification, and value-added bioproducts preparation. Light availability plays a pivotal role in Spirulina photoautotrophic cultivation, which is primary determined by characteristics of incident light and distribution of light within photobioreactors (PBRs). To clarify the role of light in Spirulina photoautotrophic cultivation, this review first analyzes the processes of light delivery and conversion in suspended PBRs. Then, effects of key light characteristics, including light intensity, spectrum, and photoperiod, on Spirulina growth and intracellular biochemical components synthesis are comprehensively summarized. Recent advancements in innovative PBR designs aimed at enhancing light utilization efficiency and promoting Spirulina growth are also highlighted. Finally, potential future research directions in the field of Spirulina photoautotrophic cultivation are outlined. Overall, this work provides a theoretical foundation and technical guidance for improving Spirulina production and specific target products synthesis from prespectives of light conditions regulation and PBRs design.
