Epidermal growth factor containing culture supernatant enhances intestine development of early-weaned pigs in vivo: Potential mechanisms involved
作者：
Bedford, Andrea;Chen, Tao;Huynh, Evanna;Zhu, Cuilan;Medeiros, Samantha;...
期刊：
Journal of Biotechnology ,2015年196-197:9-19 ISSN：0168-1656
通讯作者：
Li, Julang
作者机构：
[Medeiros, Samantha; Bedford, Andrea; de Lange, Cornelis; Zhu, Cuilan; Chen, Tao; Li, Julang; Huynh, Evanna; Wey, Doug] Univ Guelph, Dept Anim & Poultry Sci, Guelph, ON N1G 2W1, Canada.;[Chen, Tao] Hunan Agr Univ, Coll Vet Med, Changsha, Hunan, Peoples R China.
通讯机构：
[Li, Julang] U;Univ Guelph, Dept Anim & Poultry Sci, Guelph, ON N1G 2W1, Canada.
关键词：
Epidermal growth factor;Intestine development;Lactococcus lactis;Weaned pig
摘要：
We have previously generated epidermal factor expressing Lactococcus lactis (EGF-LL) using a bioengineering approach, and shown that EGF-LL fermentation supernatant enhanced newly weaned pigs growth. The objective of the current study was to further understand the mechanisms behind this improved performance. Sixty-four piglets were weaned at 3 weeks of age and then fed ad libitum according to a 2-phase feeding program. Four pens with 8 pigs per pen were assigned to each of two treatments for 3 weeks: (1) EGF containing supernatant from EGF-LL culture (SuperEGF) or (2) blank M17GE media (Control). Consistent with previous findings, SuperEGF pigs had an increased average daily gain during week 3 post-weaning (433.4. ±. 10.86 vs 388.7. ±. 7.76. g;P<. 0.05) and overall gain:feed ratio (0.757. ±. 0.03 vs 0.677. ±. 0.01. kg/kg, P<. 0.05). Moreover, jejunal structure development was enhanced, and inflammation index was minimized in SuperEGF pigs as indicated by increased villi height ( P<. 0.05), decreased lamina propria width ( P<. 0.05), and higher expression of anti-inflammatory cytokine, IL-13 ( P<. 0.05). Further, goblet cell numbers and Muc2 levels were increased in SuperEGF pigs. Interestingly, the weaning-induced decrease of glucose cotransporter sodium-glucose linked transporter 1 (SGLT1) and glucagon-like peptide-2 (GLP2) levels was reversed by SuperEGF supplementation. Our findings add to our understanding of the mechanisms behind enhancing piglet performance by EGF containing fermentation product. ©2015 Elsevier B.V.
语种：
英文
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Angiopoietin-like protein 2 may mediate the inflammation in murine mastitis through the activation of interleukin-6 and tumour necrosis factor-α
作者：
Wang, Ji-Ying;Xiao, Hong-Bo* ;Sun, Zhi-Liang;Zhang, Da-Sheng
期刊：
World Journal of Microbiology and Biotechnology ,2015年31(8):1235-1240 ISSN：0959-3993
通讯作者：
Xiao, Hong-Bo
作者机构：
[Xiao, Hong-Bo; Wang, Ji-Ying] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Sun, Zhi-Liang; Zhang, Da-Sheng] Hunan Agr Univ, Biol Vet Drugs Branch, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Xiao, Hong-Bo] H;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
关键词：
Angiopoietin-like protein 2;Murine mastitis;Staphylococcus aureus
摘要：
Mastitis is the inflammation of the mammary gland. Recent research has shown that Angiopoietin-like protein 2 (ANGPTL2) is a key inflammatory mediator. In the present study, we tested whether there is a correlation between increased ANGPTL2 expression and inflammation in response to Staphylococcus aureus in murine mastitis and the mechanisms involved. Thirty mice were divided into two groups: blank control group, challenged group. The entire infused mammary glands were removed to observe the changes of histopathology, myeloperoxidase (MPO) activity, production of tumour necrosis factor-alpha (TNF-alpha) and interleukin (IL)-6, and genes expression of ANGPTL2, TNF-alpha and IL-6. In challenged group, the structure of mammary glands was damaged and the large areas of cell fragments were observed. The MPO activity, IL-6 and TNF-alpha concentrations, ANGPTL2, IL-6, and TNF-alpha mRNA levels were significantly elevated in challenged group compared with blank control group. The present findings indicate ANGPTL2 may mediate the inflammation in murine mastitis through the activation of IL-6 and TNF-alpha.
语种：
英文
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The microRNA Signature in Response to Nutrient Restriction and Refeeding in Skeletal Muscle of Chinese Perch (Siniperca chuatsi)
作者：
Zhu, Xin;Chen, Dunxue;Hu, Yi;Wu, Ping;Wang, Kaizuo;...
期刊：
Marine Biotechnology ,2015年17(2):180-189 ISSN：1436-2228
通讯作者：
Chu, Wuying
作者机构：
[Wang, Kaizuo; Zhang, Jianshe; Zhu, Xin; Wu, Ping; Chu, Wuying; Chen, Dunxue] Changsha Univ, Dept Bioengneering & Environm Sci, Changsha 410003, Hunan, Peoples R China.;[Zhu, Xin] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Hu, Yi; Zhang, Junzhi] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Chu, Wuying] C;Changsha Univ, Dept Bioengneering & Environm Sci, Changsha 410003, Hunan, Peoples R China.
关键词：
MicroRNA;Chinese perch;Fasting and refeeding;Skeletal muscle
摘要：
The Chinese perch (Siniperca chuatsi) is one of the most commercially important carnivorous fish species in aquaculture with its large-scale culture in China. Increasing evidence suggests that microRNAs (miRNAs) play an important role in muscle cell proliferation and differentiation. However, the knowledge of the identity of myogenic miRNAs and the effect of nutrient status on miRNA expression in teleost remains limited. In the present study, among the 21 miRNAs identified with high abundance in the fast muscle of adult Chinese perch, 19 miRNAs were differentially expressed in the adults and juveniles. The postprandial changes in the transcript abundance were determined for the 21 miRNAs following a single satiating meal in the juveniles after fasting for 1 week. The results showed that the seven miRNAs (miR-10c, miR-107a, miR-133a-3p, miR-140-3p, miR-181a-5p, miR-206, and miR-214) were sharply upregulated or downregulated within 1 h after refeeding. These miRNAs may be the promising candidate miRNAs involved in a fast-response signaling system that regulates fish skeletal muscle growth. Target prediction and expressional analysis suggested that four miRNAs (miR-10c, miR-107a, miR-140-3p, and miR-181a-5p) might play a role in regulating the translation of target gene transcripts such as myostatin following acute anabolic stimuli.
语种：
英文
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Porcine circovirus type 2 affects the serum profile of amino acids and intestinal expression of amino acid transporters in mice
作者：
Xiao, Dingfu;Yin, Jie;Ren, Wenkai* ;He, Jianhua;Hu, Xionggui;...
期刊：
RSC Advances ,2015年5(90):73651-73659 ISSN：2046-2069
通讯作者：
Ren, Wenkai
作者机构：
[Xiao, Dingfu; He, Jianhua] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Yin, Jie; Ren, Wenkai; Yin, Yulong; Xiao, Dingfu] Chinese Acad Sci, Sci Observing & Expt Stn Anim Nutr & Feed Sci Sou, Hunan Prov Engn Res Ctr Hlth Livestock,Subtrop Ag, Key Lab Agroecol Proc Subtrop Reg,Minist Agr,Inst, Changsha 410125, Hunan, Peoples R China.;[Xiao, Dingfu; He, Jianhua] Hunan Collaborat Innovat Ctr Utilizat Bot Funct I, Changsha 410128, Hunan, Peoples R China.;[Hu, Xionggui] Hunan Inst Anim & Vet Sci, Changsha 410131, Hunan, Peoples R China.;[Yu, Xinglong; Luo, Wei] Hunan Agr Univ, Coll Vet Sci, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Ren, Wenkai] C;Chinese Acad Sci, Sci Observing & Expt Stn Anim Nutr & Feed Sci Sou, Hunan Prov Engn Res Ctr Hlth Livestock,Subtrop Ag, Key Lab Agroecol Proc Subtrop Reg,Minist Agr,Inst, 644 Yuanda Rd, Changsha 410125, Hunan, Peoples R China.
摘要：
PCV2 is highly pathogenic, however, its effect on the serum amino acids profile is unknown. This study was conducted to explore the profile of amino acids in the serum in porcine circovirus type 2 (PCV2) infected mice. The serum levels of amino acids were detected with isotope dilution liquid chromatography-mass spectrometry methods at 3, 7, 10 and 14 days post infection (DPI). Meanwhile, the expression of seven amino acids transporters (SLC6A14, SLC6A20, SLC7A5, SLC7A6, SLC7A7, SLC7A8, SLC7A9) in the jejunum was analyzed by reverse transcription polymerase chain reaction (RT-PCR) at 3 and 7 DPI. Serum PCV2 load was also analyzed by quantitative PCR at 3, 7, 10 and 14 DPI. Serum levels of most amino acids, such as Pro, Orn, and Met, significantly (P < 0.05) increased at 3 DPI. However, most amino acids, including Asp, Sar, Arg, Hyl, Pro, Lys, Val, Ile and Leu, significantly (P < 0.05) decreased at 7 DPI. There was no significant difference for most amino acids at 10 and 14 DPI. PCV2 infection significantly (P < 0.05) decreased expression of SLC7A5 and SLC7A6 at 7 DPI. In conclusion, PCV2 infection affects the profile of amino acids in the serum and the expression of amino acids transporters in the intestine.
语种：
英文
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基于热重法的干清猪粪直接燃烧特性分析
作者：
许道军;文利新;薛立群;蒋政云
期刊：
农业工程学报 ,2014年30(5):162-168 ISSN：1002-6819
通讯作者：
Xu, D.(xudaojun@hunau.edu.cn)
作者机构：
[薛立群; 许道军; 文利新] College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China;[蒋政云] Hunan Changsha Lü,ye Bio-Technology Co. Ltd., Changsha 410125, China
通讯机构：
College of Veterinary Medicine, Hunan Agricultural University, China
关键词：
粪便;燃烧;发热量;猪粪;生物质能源
摘要：
为了研究猪粪直接燃烧供能的可行性，该文测定了干清粪方式收集的玉米-豆粕日粮型猪粪的含水率、纤维素、半纤维素和木质素含量以及发热量；采用热重法测定了猪粪的热重曲线（thermogravimetric curves，TG curves）、微分热重曲线（diffenrential thermogravimetric curves，DTG curves），分析了猪粪的可燃性指数、燃烧稳定性指数、综合燃烧特性指数。结果表明：新鲜猪粪含水率在74.28%～76.75%；高位发热量为16.72～17.65 MJ/kg，低位发热量为14.24～15.34 MJ/kg；热重分析表明猪粪可燃性指数为（1.552～1.652）×215;10-5 mg/（min×183;℃2），燃烧稳定性指数为（1.393～1.466）×215;10-6 mg/（min×183;℃2），综合燃烧特性指数为（6.480～7.305）×215;10-9 mg2/（min2×183;℃3）。猪粪球能在锅炉内良好燃烧，呈现明显的挥发分燃烧状态。研究的结果为猪粪直接燃烧供能提供了依据和参考。
语种：
中文
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Separation and phase transition investigation of Yb3+/Er 3+ co-doped NaYF4 nanoparticles
作者：
Song, Sha;Kuang, Yun;Liu, Junfeng;Yang, Qing;Luo, Liang* ;...
期刊：
DALTON TRANSACTIONS ,2013年42(37):13315-13318 ISSN：1477-9226
通讯作者：
Luo, Liang
作者机构：
[Kuang, Yun; Luo, Liang; Liu, Junfeng; Sun, Xiaoming; Song, Sha] Beijing Univ Chem Technol, State Key Lab Chem Resource Engn, Beijing 100029, Peoples R China.;[Yang, Qing] Hunan Agr Univ, Coll Vet Med, Dept Clin Vet Med, Changsha 410128, Hunan, Peoples R China.;[Luo, Liang] Beijing Univ Chem Technol, State Key Lab Chem Resource Engn, POB 98, Beijing 100029, Peoples R China.
通讯机构：
[Luo, Liang] B;Beijing Univ Chem Technol, State Key Lab Chem Resource Engn, POB 98, Beijing 100029, Peoples R China.
关键词：
separation;transition;investigation
摘要：
Density gradient ultracentrifuge separation was employed to study the phase transition of Yb3+/Er3+ co-doped NaYF4 from α-phase to β-phase. It was found that the cubic α-phased NaYF4 was rich in Y, exhibited predominantly red luminescence, whilst the newly formed hexagonal β-phased NaYF4 had relatively higher Yb and Er atomic ratios, exhibited green luminescence most strongly. © 2013 The Royal Society of Chemistry.
语种：
英文
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Mammalian Myosin-18A, a Highly Divergent Myosin
作者：
Guzik-Lendrum, Stephanie;Heissler, Sarah M.;Billington, Neil;Takagi, Yasuharu;Yang, Yi;...
期刊：
Journal of Biological Chemistry ,2013年288(13):9532-9548 ISSN：0021-9258
通讯作者：
Sellers, James R.
作者机构：
[Homsher, Earl; Sellers, James R.] NHLBI, Lab Mol Physiol, NIH, Bethesda, MD 20892 USA.;[Yang, Yi] Hunan Agr Univ, Coll Vet Med, Lab Funct Prote, Changsha 410128, Hunan, Peoples R China.;[Knight, Peter J.] Univ Leeds, Astbury Ctr Struct Mol Biol, Sch Mol & Cellular Biol, Leeds LS2 9JT, W Yorkshire, England.;[Homsher, Earl] Univ Calif Los Angeles, David Geffen Sch Med, Dept Physiol, Los Angeles, CA 90095 USA.;[Sellers, James R.] NHLBI, Lab Mol Physiol, NIH, 50 South Dr,B50-3523, Bethesda, MD 20892 USA.
通讯机构：
[Sellers, James R.] N;NHLBI, Lab Mol Physiol, NIH, 50 South Dr,B50-3523, Bethesda, MD 20892 USA.
摘要：
The Musmusculus myosin-18A gene is expressed as two alternatively spliced isoforms, αand β, with reported roles in Golgi localization, in maintenance of cytoskeleton, and as receptors for immunological surfactant proteins. Both myosin-18A isoforms feature a myosin motor domain, a single predicted IQ motif, and a long coiled-coil reminiscent of myosin-2. The myosin-18Aαisoform, additionally, has an N-terminal PDZ domain. Recombinant heavy meromyosin- and subfragment-1 (S1)-like constructs for both myosin-18Aαand -18βspecies were purified from the baculovirus/Sf9 cell expression system. These constructs bound both essential and regulatory light chains, indicating an additional noncanonical light chain binding site in the neck. Myosin-18Aα-S1 and -18Aβ-S1 molecules bound actin weakly with Kd values of 4.9 and 54 μM, respectively. The actin binding data could be modeled by assuming an equilibrium between two myosin conformations, a competent and an incompetent form to bind actin. Actin binding was unchanged by presence of nucleotide. Both myosin-18A isoforms bound N-methylanthraniloyl- nucleotides, but the rate of ATP hydrolysis was very slow (<0.002 s <sup>-1</sup>) and not significantly enhanced by actin. Phosphorylation of the regulatory light chain had no effect on ATP hydrolysis, and neither did the addition of tropomyosin or of GOLPH3, a myosin-18A binding partner. Electron microscopy of myosin-18A-S1 showed that the lever is strongly angled with respect to the long axis of the motor domain, suggesting a pre-power stroke conformation regardless of the presence of ATP. These data lead us to conclude that myosin-18A does not operate as a traditional molecular motor in cells. ©2013 by The American Society for Biochemistry and Molecular Biology, Inc.
语种：
英文
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An introduction to hybrid ion trap/time-of-flight mass spectrometry coupled with liquid chromatography applied to drug metabolism studies
作者：
Liu, Zhao-Ying*
期刊：
JOURNAL OF MASS SPECTROMETRY ,2012年47(12):1627-1642 ISSN：1076-5174
通讯作者：
Liu, Zhao-Ying
作者机构：
[Liu, Zhao-Ying] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liu, Zhao-Ying] H;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
关键词：
LC-IT-TOF-MS;accurate mass measurements;drug metabolism;elemental compositions;structural characterization
摘要：
Metabolism studies play an important role at various stages of drug discovery and development. Liquid chromatography combined with mass spectrometry (LC/MS) has become a most powerful and widely used analytical tool for identifying drug metabolites. The suitability of different types of mass spectrometers for metabolite profiling differs widely, and therefore, the data quality and reliability of the results also depend on which instrumentation is used. As one of the latest LC/MS instrumentation designs, hybrid ion trap/time-of-flight MS coupled with LC (LC-IT-TOF-MS) has successfully integrated ease of operation, compatibility with LC flow rates and data-dependent MS<sup>n</sup> with high mass accuracy and mass resolving power. The MS<sup>n</sup> and accurate mass capabilities are routinely utilized to rapidly confirm the identification of expected metabolites or to elucidate the structures of uncommon or unexpected metabolites. These features make the LC-IT-TOF-MS a very powerful analytical tool for metabolite identification. This paper begins with a brief introduction to some basic principles and main properties of a hybrid IT-TOF instrument. Then, a general workflow for metabolite profiling using LC-IT-TOF-MS, starting from sample collection and preparation to final identification of the metabolite structures, is discussed in detail. The data extraction and mining techniques to find and confirm metabolites are discussed and illustrated with some examples. This paper is directed to readers with no prior experience with LC-IT-TOF-MS and will provide a broad understanding of the development and utility of this instrument for drug metabolism studies. ©2012 John Wiley &Sons, Ltd.
语种：
英文
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Research on the relationship between customer value of social network service and customer loyalty
作者：
Xiao, Lihui* ;Wang, Fang;Wang, Zhuang
期刊：
Advances in Intelligent and Soft Computing ,2012年117 AISC(127 VOL. 2):27-32 ISSN：1867-5662
通讯作者：
Xiao, Lihui
作者机构：
[Xiao, Lihui; Wang, Fang] Hunan Agr Univ, Coll Business, Changsha, Hunan, Peoples R China.;[Wang, Zhuang] Hunan Agr Univ, Coll Vet Med, Changsha, Hunan, Peoples R China.
通讯机构：
[Xiao, Lihui] H;Hunan Agr Univ, Coll Business, Changsha, Hunan, Peoples R China.
会议名称：
3rd International Conference on Teaching and Computational Science (WTCS)
会议时间：
DEC 19-20, 2009
会议地点：
Shenzhen, PEOPLES R CHINA
会议主办单位：
[Xiao, Lihui;Wang, Fang] Hunan Agr Univ, Coll Business, Changsha, Hunan, Peoples R China.^[Wang, Zhuang] Hunan Agr Univ, Coll Vet Med, Changsha, Hunan, Peoples R China.
会议论文集名称：
Advances in Intelligent and Soft Computing
关键词：
customer loyalty;customer value;SNS Website;trust
摘要：
An increasing number of people are processing transactions online and the numbers are likely to increase rapidly in the near future. It is important to analyze the relationship between customer values of social network service and Customer Loyalty. The purpose of this paper is to answer the following questions. First, what are key components of customer value in social network service? Second, what influences the relationship between customer value and Customer Loyalty? Data collected from 160 respondents who participate in social network service were used to test a research model. Several managerial implications were derived from the analysis and further studies were suggested. ©2012 Springer Science+Business Media Dordrecht.
语种：
英文
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PROTECTIVE EFFECTS OF SOLUBLE FIBER FROM UNDARIA PINNATIFIDA ON VASCULAR ENDOTHELIUM IN MICE
作者：
Xiao, Hong-Bo* ;Lu, Xiang-Yang;Sun, Zhi-Liang;Hu, Ya-Ping;Bai, Xia;...
期刊：
Journal of Food Biochemistry ,2011年35(6):1737-1746 ISSN：0145-8884
通讯作者：
Xiao, Hong-Bo
作者机构：
[Xiao, Hong-Bo; Bai, Xia; Zhang, Ming-Jun.; Chen, Xiao-Jun] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Lu, Xiang-Yang] Hunan Agr Univ, Hunan Agr Bioengn Res Inst, Changsha 410128, Hunan, Peoples R China.;[Sun, Zhi-Liang] Hunan Agr Univ, Hunan Vet Drug Engn Technol Ctr, Changsha 410128, Hunan, Peoples R China.;[Hu, Ya-Ping] Hunan Agr Univ, Fac Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Xiao, Hong-Bo] H;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
摘要：
The objective of this study was to investigate the protective effect of soluble fiber extracted from Undaria pinnatifida (UP) on vascular endothelium and the mechanisms involved. The experiments were performed in aorta and plasma from C57BL/6J control and apolipoprotein E deficient (ApoE<sup>-/-</sup>) mice treated or not with UP soluble fiber (5 or 10%) for 8 weeks. UP soluble fiber treatment improved endothelium-dependent vasorelaxation, increased the maximal relaxation value, and decreased the half-maximum effective concentration concomitantly with a decrease in asymmetric dimethylarginine (ADMA) and malondialdehyde plasma concentrations, and increase in the expression as well as activity of dimethylarginine dimethylaminohydrolase II in ApoE<sup>-/-</sup> mice. Treatment with UP soluble fiber also significantly decreased reactive oxygen species production in mice aorta. The present results suggest that the protective effect of UP soluble fiber on vascular endothelium may be associated with a decrease in ADMA level in mice. PRACTICAL APPLICATIONS: Soluble fiber have complex beneficial effects on cardiovascular system such as lowering serum cholesterol, inhibiting lipid peroxidation, reducing blood pressure, increasing insulin sensitivity, modulating plasma glucose, improving homocysteine levels, protecting against intima-media thickness progression of the common carotid arteries, and decreasing the serum levels of markers of inflammation. The present work reports that the protective effect of Undaria pinnatifida soluble fiber on vascular endothelium may be associated with a decrease in ADMA level. It complements previous work. This is important because the mechanism responsible for the protective effect of fiber on endothelial function is defined. Furthermore, it provides information for the consumer about the potential effects of fiber and Undaria pinnatifida. ©2011 Wiley Periodicals, Inc.
语种：
英文
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An integrated method for degradation products detection and characterization using hybrid ion trap/time-of-flight mass spectrometry and data processing techniques: Application to study of the degradation products of danofloxacin under stressed conditions
作者：
Liu, Zhao-Ying;Zhou, Xiao-Ni;Zhang, Hua-Hai;Wan, Leren;Sun, Zhi-Liang*
期刊：
Analytical and Bioanalytical Chemistry ,2011年399(7):2475-2486 ISSN：1618-2642
通讯作者：
Sun, Zhi-Liang
作者机构：
[Sun, Zhi-Liang; Liu, Zhao-Ying; Zhou, Xiao-Ni] Hunan Agr Univ, Coll Vet Med, Hunan Engn Res Ctr Vet Drugs, Changsha 410128, Hunan, Peoples R China.;[Zhang, Hua-Hai] NW A&F Univ, Coll Forestry, Yangling 712100, Shanxi, Peoples R China.;[Wan, Leren] Shimadzu Int Trading Shanghai Co Ltd, Shanghai 200052, Peoples R China.
通讯机构：
[Sun, Zhi-Liang] H;Hunan Agr Univ, Coll Vet Med, Hunan Engn Res Ctr Vet Drugs, Changsha 410128, Hunan, Peoples R China.
关键词：
Ion trap/time-of-flight mass spectrometry;Degradation product;Accurate mass measurements;Structural characterization;Danofloxacin
摘要：
A new strategy using hybrid ion trap/time-of-flight mass spectrometry coupled with high-performance liquid chromatography and post-acquisition data mining techniques was developed and applied to the detection and characterization of degradation products of danofloxacin. The degradation products formed under different forced conditions were separated using an ODS-C18 column with gradient elution. Accurate full-scan MS data were acquired in the first run and processed with the combination of extracted ion chromatograms and LC-UV chromatograms. These processes were able to find accurate molecular masses of possible degradation products. Then, the accurate MS/MS data acquired through data-dependent analysis mode in another run facilitated the structural elucidations of degradation products. As a result, a total of 11 degradation products of danofloxacin were detected and characterized using the developed method. Overall, this analytical strategy enables the acquisition of accurate-mass LC/MS data, search of a variety of degradation products through the post-acquisition processes, and effective structural characterization based on elemental compositions of degradation product molecules and their product ions. The ability to measure degradation products via tandem mass spectrometry coupled with accurate mass measurement, all in only two experimental runs, is one of the most attractive features of this methodology. The results demonstrate that use of the LC/MS-IT-TOF approach appears to be rapid, efficient and reliable in structural characterization of drug degradation products. [Figure not available: see fulltext.] © 2011 Springer-Verlag.
语种：
英文
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Improved degradation of organophosphate dichlorvos by Trichoderma atroviride transformants generated by restriction enzyme-mediated integration (REMI)
作者：
Tang, Jun;Liu, Lixing;Hu, Shifeng;Chen, Yunpeng;Chen, Jie*
期刊：
Bioresource Technology ,2009年100(1):480-483 ISSN：0960-8524
通讯作者：
Chen, Jie
作者机构：
[Liu, Lixing; Chen, Yunpeng; Tang, Jun; Hu, Shifeng; Chen, Jie] Shanghai Jiao Tong Univ, Minist Educ, Dept Plant Sci, Key Lab Microorganism Metab,Sch Agr & Biol, Shanghai 201101, Peoples R China.;[Hu, Shifeng] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Chen, Jie] S;Shanghai Jiao Tong Univ, Minist Educ, Dept Plant Sci, Key Lab Microorganism Metab,Sch Agr & Biol, Shanghai 201101, Peoples R China.
关键词：
REMI;Trichoderma;Organophosphate pesticide;Dichlorvos;Biodegradation
摘要：
A simple technique, REMI (restriction enzyme-mediated integration), was used to construct transformants of Trichoderma atroviride with improved capability of degrading organophosphate pesticide dichlorvos. Linearized DNA of plasmid pV2 bearing the hygromycin B phosphotransferase (hph) gene was inserted into chromosomes of wild strain T23 and transformation was confirmed by PCR and Southern blot analysis, respectively. Of 247 transformants, 76% showed improved dichlorvos degradation ability as compared to the parent strain T23 based on the least significant difference (LSD) test at p = 0.01. Among them, 8 transformants exhibited 30% higher in degradation rate than the parent isolate. The highest dichlorvos degradation rate of the transformants was up to 96%. This study provided an effective approach for improving organophosphate pesticide-degrading capability of T. atroviride. © 2008 Elsevier Ltd. All rights reserved.
语种：
英文
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高炉并罐式无钟炉顶的均匀布料法
作者：
杨亚;刘进辉;伏健;严芬;程天印
期刊：
钢铁 ,1993年28(4):5-10 ISSN：0449-749X
通讯作者：
Liu, Yuncai
作者机构：
[杨亚; 刘进辉; 伏健; 严芬; 程天印] 湖南农业大学动物医学院
通讯机构：
Shoudu Iron and Steel Co, China
关键词：
无钟炉顶;高炉;布料;炉料;溜槽;偏斜;转速;参数计算;轨迹
摘要：
蜱类系统分类学研究已经进入分子阶段,与传统的形态学、生物学鉴定相比,无疑更加客观准确,建立系统进化树更能全面反映其亲缘关系,极大地提高了鉴定效率与可信度。论文总结了近年来应用分子生物学技术鉴定硬蜱的主要方法,包括核酸序列分析、随机扩增DNA多态性分析、限制性片段长度多态性分析。随着分子生物学和现代统计学的广泛应用,蜱类的鉴定将更加依赖现代分类学方法。
语种：
中文
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