作者机构：
[Wu, Haoyang; Wang, Jinyu; Peng, Yi; Lv, Peiyuan; Lin, Jiaji] Department of Neurology, The Second Affiliated Hospital of Air Force Medical University, Xi'an, China;[Wu, Haoyang; Wang, Jinyu; Lv, Peiyuan; Lin, Jiaji] Basic Medical School, Air Force Medical University, Xi'an, China;[Bennett, Brian] Integrative Bioinformatics Support Group, National Institute of Environmental Health Sciences, Durham, United States;[Wang, Jiajia] Computer Network Information Center, Chinese Academy of Sciences, Beijing, China;[Li, Pishun] College of Veterinary Medicine, Hunan Agricultural University, Changsha, China
摘要：
Epigenetic regulators present novel opportunities for both ischemic stroke research and therapeutic interventions. While previous work has implicated that they may provide neuroprotection by potentially influencing coordinated sets of genes and pathways, most of them remain largely uncharacterized in ischemic conditions. In this study, we used the oxygen-glucose deprivation (OGD) model in the immortalized mouse hippocampal neuronal cell line HT-22 and carried out an RNAi screen on epigenetic regulators. PRMT5 was identified as a novel negative regulator of neuronal cell survival after OGD, which presented a phenotype of translocation from the cytosol to the nucleus upon oxygen and energy depletion both in vitro and in vivo. PRMT5 bound to the chromatin and a large number of promoter regions to repress downstream gene expression. Silencing Prmt5 significantly dampened the OGD-induced changes for a large-scale of genes, and gene ontology analysis showed that PRMT5-target genes were highly enriched for Hedgehog signaling. Encouraged by the above observation, mice were treated with middle cerebral artery occlusion with the PRMT5 inhibitor EPZ015666 and found that PRMT5 inhibition sustains protection against neuronal death in vivo. Together, these findings revealed a novel epigenetic mechanism of PRMT5 in cerebral ischemia and uncovered a potential target for neuroprotection.
摘要：
Acute colitis is a complex disease that can lead to dysregulation of the gut flora, inducing more complex parenteral diseases. Dandelion polysaccharides (DPSs) may have potential preventive and therapeutic effects on enteritis. In this study, LPS was used to induce enteritis and VC was used as a positive drug control to explore the preventive and therapeutic effects of DPS on enteritis. The results showed that DPS could repair the intestinal barrier, down-regulate the expression of TNF-α, IL-6, IL-1β, and other pro-inflammatory factors, up-regulate the expression of IL-22 anti-inflammatory factor, improve the antioxidant capacity of the body, and improve the structure of intestinal flora. It is proved that DPS can effectively prevent and treat LPS-induced acute enteritis and play a positive role in promoting intestinal health.
期刊：
Journal of Traditional and Complementary Medicine,2024年 ISSN：2225-4110
通讯作者：
Ji Wang
作者机构：
[Xiangyan Liu; Ran Tao; Fangrui Guo; Linyu Zhang; Jianyu Qu; Mengyao Li; Xiaoran Wu; Yuanyuan Zhu; Lixin Wen] Hunan Engineering Research Center of Livestock and Poultry Health Care, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China;[Xianglin Wang] Changsha Lvye Biotechnology Co., Ltd., Changsha, 410100, China;Animal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha, 410128, China;[Ji Wang] Hunan Engineering Research Center of Livestock and Poultry Health Care, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China<&wdkj&>Animal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha, 410128, China
通讯机构：
[Ji Wang] H;Hunan Engineering Research Center of Livestock and Poultry Health Care, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China<&wdkj&>Animal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha, 410128, China
摘要：
Neuroinflammation is considered the principal pathogenic mechanism underlying neurodegenerative diseases, and the incidence of brain disorders is closely linked to dietary fat consumption and intestinal health. To investigate this relationship, 60 8-week-old C57BL/6J mice were subjected to a 20-week dietary intervention, wherein they were fed lard and soybean oil, each at 15% and 35% fat energy. At a dietary fat energy level of 35%, inflammation was observed in both the soybean oil and lard groups. Nevertheless, inflammation was more pronounced in the mice that were administered soybean oil. The process by which nerve cell structure is compromised, inflammatory factors are upregulated, brain antioxidant capacity is diminished, and the TLR4/MyD88/NF-κB p65 inflammatory pathway is activated resulting in damage to the brain-gut barrier. This, in turn, leads to a reduction in the abundance of Akkermansia and unclassified_f_Lachnospiraceae, as well as an increase in Dubosiella abundance, ultimately resulting in brain inflammation and damage. These results suggested that soybean oil induces more severe neuroinflammation compared to lard. Our study demonstrated that, at a dietary fat energy level of 35%, compared to soybean oil, lard could be the healthier option, the outcomes would help provide a reference basis for the selection of residents’ daily dietary oil.
摘要：
Efforts to curtail the escalating health threat posed by methicillin-resistant Staphylococcus aureus (MRSA), a formidable superbug, necessitate the development of innovative treatment strategies. Leveraging potential compounds from natural sources in tandem with antibiotics has emerged as a promising approach against MRSA. These strategies should enhance the antibiotic efficacy, reduce dosage and toxicity, and bypass MRSA resistance. In this study, we used a checkerboard assay to illustrate the significant synergistic anti-MRSA effect of shikimic acid (SA), a naturally occurring compound, and ceftiofur (CF). Time-kill curves further revealed that a combination of 1/4 of the minimum inhibitory concentration (MIC) of SA and 1/8 MIC of the sodium CF eradicated MRSA within 2h, with no noticeable toxicity observed with these concentrations. In vivo experiments confirmed that this combination therapy demonstrated robust antimicrobial activity against MRSA-induced bacteremia in mice, significantly reducing bacterial loads in the kidneys, liver, and spleen, attenuating inflammatory cell infiltration, and alleviating pathological damage. This study not only offers a compelling strategy, capitalizing on the synergistic potential of SA and CF, to rapidly address antibiotic resistance but also contributes significantly to the refinement of antimicrobial therapeutic strategies.
作者：
Li Kong;Xinyu Gao;Lijuan Zhu;Xing Lin;You Huang;...
期刊：
食品科学与人类健康,2024年13(3):1292-1302 ISSN：2213-4530
通讯作者：
Zhihang Yuan<&wdkj&>Jin’e Yi
作者机构：
[Li Kong; Xinyu Gao; Lijuan Zhu; Xing Lin; You Huang; Chunlin Huang; Wenjiang Yang; Yazhi Chen; Haoqiang Zhao; Jing Wu; Zhihang Yuan; Jin’e Yi] Hunan Engineering Research Center of Livestock and Poultry Health Care, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China
通讯机构：
[Zhihang Yuan; Jin’e Yi] H;Hunan Engineering Research Center of Livestock and Poultry Health Care, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China
摘要：
F-2 toxin is an estrogenic mycotoxin that causes reproductive disorders in animals. Betulinic acid (BA) is a natural pentacyclic lupane-structure triterpenoid that has diverse pharmacological activities. In this study, the antioxidative and anti-inflammatory effects of BA and its underlying mechanism are explored in F-2 toxin-triggered mouse ovarian damage. We found that BA alleviated the F-2 toxin-induced ovarian impairment by stimulating follicle growth, reducing inflammatory cell infiltration, repairing damaged mitochondria and endoplasmic reticulum. Simultaneously, BA not only reversed F-2 toxin-induced reduction of follicle stimulating hormone (FSH) and luteinizing hormone (LH) levels in the serum, but also restrained the protein expression of the estrogen receptors α (ERα) and ERβ. Moreover, BA restored the balance of F-2 toxin-induced ovarian redox system disorders. Subsequently, we found that 0.25 mg/kg BA played an anti-inflammatory role in the F-2 toxin-induced ovarian impairment by decreasing interleukin-1β (IL-1β), IL-6, and tumor necrosis factor-α (TNF-α) mRNA expression, as well as inhibiting p38 protein expression. These data demonstrated that BA exerts its protective effect on F-2 toxin-induced ovarian oxidative impairment and inflammation by inhibiting p38 expression, which implies a natural product-based medicine to ameliorate F-2 toxin-caused female reproductive toxicity and provides a detoxifying method for food contaminated by mycotoxin.
摘要：
Pseudorabies virus (PRV) is one of the major viral pathogens leading to reproductive disorders in swine. However, little is known about the effects of PRV infection on porcine reproductive system. Ovarian granulosa cells are somatic cells surrounding oocytes in ovary and required for folliculogenesis. The present study aimed to investigate the interference of PRV on functions of porcine ovarian granulosa cells in vitro. Primary granulosa cells were isolated from porcine ovaries. To investigate the PRV infectivity, transmission electron microscopy (TEM) was used to check the presence of viral particles, and the expression of viral gE gene was detected by quantitative real-time PCR (qPCR) in PRV-inoculated cells. After PRV infection, cell viability was detected by MTS assay, Ki67 for proliferative status was determined by immunofluorescence assay (IFA), cell cycle and apoptosis were detected by flow cytometry, and progesterone (P4) and estradiol (E2) were determined by radioimmunoassay. The checkpoint genes of cell cycle and apoptosis-related proteins were studied by qPCR and western blotting. Virus particles were observed in the nucleus and cytoplasm of PRV-infected granulosa cells by TEM imaging, and the expression of viral gE gene increased in a time-dependent manner post infection. PRV infection inhibited cell viability and blocked cell cycle at S phase in porcine granulosa cells, accompanied by decreases in expression of Ki67 protein and checkpoint genes related to S phase. Radioimmunoassay revealed decreased levels in P4 and E2, and the expressions of key steroidogenic enzymes were also down-regulated post PRV-infection. In addition, PRV induced apoptosis with an increase in Bax expression and activation of caspase 9, and the phosphorylation of JNK, ERK and p38 MAPKs were significantly up-regulated in porcine ovarian granulosa cells post PRV infection. The data indicate that PRV causes infection on porcine ovarian granulosa cells and interferes the cell functions through apoptosis, and the MAPK signaling pathway is involved in the viral pathogenesis.
期刊：
International Immunopharmacology,2024年129:111653 ISSN：1567-5769
通讯作者：
Jing Wu<&wdkj&>Chenglin Yang
作者机构：
[Wang, Siqi; Li, Yuanyuan; Ning, Can; Fan, Hui; Xiao, Wenguang; Wu, You; Yuan, Zhihang; Wang, Yongkang; Kong, Xiangyi; Wu, Aoao] Hunan Engineering Research Center of Livestock and Poultry Health Care, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China;[Liang, Zengenni] Hunan Agricultural Product Processing Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China;[Liang, Zengenni] Longping Branch Graduate School, Hunan University, Changsha 410125, China;[Wu, Jing] Hunan Engineering Research Center of Livestock and Poultry Health Care, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China. Electronic address: wujing@hunau.edu.cn;[Yang, Chenglin] Hunan Engineering Research Center of Livestock and Poultry Health Care, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China. Electronic address: chenglin4698@163.com
通讯机构：
[Jing Wu; Chenglin Yang] H;Hunan Engineering Research Center of Livestock and Poultry Health Care, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China
摘要：
T-2 toxin, an unavoidable contaminant in animal feeds, can induce oxidative stress and damage immune organs. Melatonin (MT), a natural and potent antioxidant, has shown promise as a detoxifier for various mycotoxins. However, the detoxifying effect of MT on T-2 toxin has not been previously reported. In order to investigate the protective effect of MT added to diets on the immune system of T-2 toxin-exposed piglets, twenty piglets weaned at 28d of age were randomly divided into control, T-2 toxin (1mg/kg), MT (5mg/kg), and T-2 toxin (1mg/kg)+MT (5mg/kg) groups(n=5 per group). Our results demonstrated that MT mitigated T-2 toxin-induced histoarchitectural alterations in the spleen and thymus, such as hemorrhage, decreased white pulp size in the spleen, and medullary cell sparing in the thymus. Further research revealed that MT promoted the expression of Nrf2 and increased the activities of antioxidant enzymes CAT and SOD, while reducing the production of the lipid peroxidation product MDA. Moreover, MT inhibited the NF-κB signaling pathway, regulated the expression of downstream cytokines IL-1β, IL-6, TNF-α, and TGF-β1. MT also suppressed the activation of caspase-3 while down-regulating the ratio of Bax/Bcl-2 to reduce apoptosis. Additionally, MT ameliorated the T-2 toxin-induced disorders of immune cells and immune molecules in the blood. In conclusion, our findings suggest that MT may effectively protect the immune system of piglets against T-2 toxin-induced damage by inhibiting oxidative stress, inflammatory response, and apoptosis in the spleen and thymus. Therefore, MT holds the potential as an antidote for T-2 toxin poisoning.
期刊：
Postharvest Biology and Technology,2024年212:112890 ISSN：0925-5214
通讯作者：
Shang Guo
作者机构：
[Hongrui Lv; Zhenzhen Wu; Xiaojie Nan; Min Zhu; Kai Mao] Shanxi Institute for Functional Food, Shanxi Agricultural University, Taiyuan 030031, China;College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China;[Shang Guo] Shanxi Institute for Functional Food, Shanxi Agricultural University, Taiyuan 030031, China<&wdkj&>College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China
通讯机构：
[Shang Guo] S;Shanxi Institute for Functional Food, Shanxi Agricultural University, Taiyuan 030031, China<&wdkj&>College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China
摘要：
Daylily flower buds are a good source of nutrients and contain lots of bioactive compounds, but they are subject to deterioration after harvest, which limits the taste and consumer satisfaction. Hydrogen sulfide (H2S) has been regarded as an important gaseous signaling molecule, which could prolong postharvest freshness of fruits and vegetables. The present study aims to investigate the effects of H2S on postharvest quality and metabolism changes of daylily flower buds during storage. The results showed that 3.2 mM NaHS (H2S donor) effectively inhibited weight loss, blooming and decay of daylily during storage. H2S application markedly reduced the accumulation of malondialdehyde and H2O2. H2S treatment also delayed the loss of total protein and antioxidant glutathione, and maintained the activities of antioxidant enzymes superoxide dismutase and catalase at higher level compared to control group. Widely targeted metabolomics identified various differentially accumulated metabolites (DAMs) during storage and with H2S treatment of daylily. These DAMs largely distributed in the classes of amino acids, organic acids, sugars and alcohols, terpenoids. A special focus was given to 303 common DAMs between the comparison of CK0 vs. CK6 and CK6 vs. T6, of which the DAMs were mainly enriched in the KEGG pathways of monobactam biosynthesis, pyrimidine metabolism, glycine, serine and threonine metabolism, arginine biosynthesis, pentose and glucuronate interconversions. Four DAMs including Cytidine 5′-Monophosphate, Fumaric Acid, Hydroxypyruvic Acid, 2′-Deoxycytidine-5′-Monophosphoric Acid exhibited great downregulation during storage, and H2S treatment dramatically recovered their relative contents. This study indicated that H2S treatment offers the potential to preserve the quality of daylily flower buds and extend their postharvest life.
摘要：
<bold>Background:</bold> Chlamydia is a Gram-negative obligate intracellular bacterium that is pathogenic for humans and a large variety of veterinary animal species. However, there is no continuous monitoring of chlamydia infection data in pigs in Hunan province, southern China. Therefore, in order to evaluate the seroprevalence and identify risk factors associated with Chlamydia infection in pigs within this region, a comprehensive study was conducted. <bold>Methods:</bold> A total of 3848 serum samples were collected from pigs (from farmers and companies) between May 2017 and August 2018. The presence of specific antibodies against Chlamydia was determined through the employment of the indirect hemagglutination assay (IHA). <bold>Results:</bold> The overall seroprevalence of Chlamydia was determined to be 26.90% (1038/3848, 95% confidence interval: 25.60-28.40). By employing statistical analysis using SPSS software (p < 0.05), factors such as altitude, sampling regions, and rearing systems of pigs were identified as potential risk factors for Chlamydia infection. <bold>Conclusion:</bold> These findings elucidate a substantial prevalence of Chlamydia in pigs within the mountainous region of Hunan province, southern China, thereby highlighting a potential risk to human health. These results underscore the need for proactive measures and targeted interventions to mitigate the transmission of Chlamydia in porcine populations, safeguarding both animal welfare and public health.
作者机构：
National Center for International Research on Animal Genetics, Breeding and Reproduction (NCIRAGBR), Ministry of Science and Technology of the People’s Republic of China, Huazhong Agricultural University, Wuhan 430070, China;Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;Author to whom correspondence should be addressed.;[Nie, Pei] College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China;[Zou, Wenna; Zhang, Xinxin; Zhang, Yiping; Yao, Zhiqiu; Li, Yuan] National Center for International Research on Animal Genetics, Breeding and Reproduction (NCIRAGBR), Ministry of Science and Technology of the People’s Republic of China, Huazhong Agricultural University, Wuhan 430070, China<&wdkj&>Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
通讯机构：
[Liguo Yang] N;National Center for International Research on Animal Genetics, Breeding and Reproduction (NCIRAGBR), Ministry of Science and Technology of the People’s Republic of China, Huazhong Agricultural University, Wuhan 430070, China<&wdkj&>Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China<&wdkj&>Author to whom correspondence should be addressed.
摘要：
Mastitis is one of the most predominant diseases with a negative impact on ranch products worldwide. It reduces milk production, damages milk quality, increases treatment costs, and even leads to the premature elimination of animals. In addition, failure to take effective measures in time will lead to widespread disease. The key to reducing the losses caused by mastitis lies in the early detection of the disease. The application of deep learning with powerful feature extraction capability in the medical field is receiving increasing attention. The main purpose of this study was to establish a deep learning network for buffalo quarter-level mastitis detection based on 3054 ultrasound images of udders from 271 buffaloes. Two data sets were generated with thresholds of somatic cell count (SCC) set as 2 × 10(5) cells/mL and 4 × 10(5) cells/mL, respectively. The udders with SCCs less than the threshold value were defined as healthy udders, and otherwise as mastitis-stricken udders. A total of 3054 udder ultrasound images were randomly divided into a training set (70%), a validation set (15%), and a test set (15%). We used the EfficientNet_b3 model with powerful learning capabilities in combination with the convolutional block attention module (CBAM) to train the mastitis detection model. To solve the problem of sample category imbalance, the PolyLoss module was used as the loss function. The training set and validation set were used to develop the mastitis detection model, and the test set was used to evaluate the network's performance. The results showed that, when the SCC threshold was 2 × 10(5) cells/mL, our established network exhibited an accuracy of 70.02%, a specificity of 77.93%, a sensitivity of 63.11%, and an area under the receiver operating characteristics curve (AUC) of 0.77 on the test set. The classification effect of the model was better when the SCC threshold was 4 × 10(5) cells/mL than when the SCC threshold was 2 × 10(5) cells/mL. Therefore, when SCC ≥ 4 × 10(5) cells/mL was defined as mastitis, our established deep neural network was determined as the most suitable model for farm on-site mastitis detection, and this network model exhibited an accuracy of 75.93%, a specificity of 80.23%, a sensitivity of 70.35%, and AUC 0.83 on the test set. This study established a 1/4 level mastitis detection model which provides a theoretical basis for mastitis detection in buffaloes mostly raised by small farmers lacking mastitis diagnostic conditions in developing countries.
摘要：
Ticks are blood-sucking ectoparasites that secrete immunomodulatory substances in saliva to hosts during engorging. Cystatins, a tick salivary protein and natural inhibitor of Cathepsins, are attracting growing interest globally because of the immunosuppressive activities and the feasibility as an antigen for developing anti-tick vaccines. This review outlines the classification and the structure of tick Cystatins, and focuses on the anti-inflammatory effects and molecular mechanisms. Tick Cystatins can be divided into four families based on structures and cystatin 1 and cystatin 2 are the most abundant. They are injected into hosts during blood feeding and effectively mitigate the host inflammatory response. Mechanically, tick Cystatins exert anti-inflammatory properties through the inhibition of TLR-NF-κb, JAK-STAT and p38 MAPK signaling pathways. Further investigations are crucial to confirm the reduction of inflammation in other cell types like neutrophils and mast cells, and fully elucidate the underlying mechanism (like the structural mechanism) to make Cystatin a potential candidate for the development of novel anti-inflammation agents.
作者机构：
Hunan Key Laboratory of Traditional Chinese Veterinary Medicine, Hunan Agricultural University , Changsha 410128, Hunan, China;College of Veterinary Medicine, Hunan Agricultural University , Changsha 410128, Hunan, China;[Zhou, Li; Song, Rong] Hunan Institute of Agricultural Environment and Ecology, Hunan Academy of Agricultural Sciences , Changsha 410125, Hunan, China;[Zhong, Xiaohong] College of Horticulture, Hunan Agricultural University , Changsha 410128, Hunan, China;National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resource and Initiative, Hunan Agricultural University , Changsha 410128, Hunan, China
通讯机构：
[Peng Huang] C;[Jianguo Zeng] H;Hunan Key Laboratory of Traditional Chinese Veterinary Medicine, Hunan Agricultural University , Changsha 410128, Hunan, China<&wdkj&>College of Veterinary Medicine, Hunan Agricultural University , Changsha 410128, Hunan, China<&wdkj&>National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resource and Initiative, Hunan Agricultural University , Changsha 410128, Hunan, China<&wdkj&>College of Animal Science and Technology, Hunan Agricultural University , Changsha 410128, Hunan, China
摘要：
The Golden apple snail, Pomacea canaliculata, is one of the world's 100 worst invasive alien species that is best known for its damage to wetland agriculture. We analyzed the genetic variation and population genetics of P. canaliculata populations in seven regions of China based on molecular markers of three mitochondrial (mt) genes. Abstract The Golden apple snail, Pomacea canaliculata, is one of the world's 100 worst invasive alien species that is best known for its damage to wetland agriculture. It also acts as an intermediate host of some zoonotic parasites such as Angiostrongylus cantonensis, posing threats to human public health and safety. Despite is being an important agricultural pest, the genetic information and population expansion history of this snail remains poorly understood in China. In this study, we analyzed the genetic variation and population genetics of P. canaliculata populations in seven regions of China based on molecular markers of three mitochondrial (mt) genes. A total of 15 haplotypes were recognized based on single mt cox1, nad1, and nad4, and eight haplotypes were identified using the concatenated genes. High haplotype diversity, moderate nucleotide diversity, low gene flow, and high rates of gene differentiation among the seven P. canaliculata populations were detected. Shanghai and Yunnan populations showed higher genetic flow and very low genetic differentiation. The results of Tajima's D, Fu's Fs, and mismatch distribution showed that P. canaliculata did not experience population expansion in China. Genetic distance based on haplotypes suggested that nad1 gene was more conserved than cox1 gene within P. canaliculata. The phylogenetic analyses showed there may be two geographical lineages in the Chinese mainland. The present study may provide a new genetic marker to analyze P. canaliculata, and results support more evidence for studying the genetic distribution of P. canaliculata in China and contribute to a deeper understanding of its population genetics and evolutionary biology.
摘要：
Evidence showed that N6-methyladenosine (m6A) modification plays a pivotal role in influencing RNA fate and is strongly associated with cell growth and developmental processes in many species. However, no information regarding m6A modification in Eimeria tenella is currently available. In the present study, we surveyed the transcriptome-wide prevalence of m6A in sporulated oocysts and unsporulated oocysts of E. tenella. Methylated RNA immunoprecipitation sequencing (MeRIP-seq) analysis showed that m6A modification was most abundant in the coding sequences, followed by stop codon. There were 3,903 hypermethylated and 3,178 hypomethylated mRNAs in sporulated oocysts compared with unsporulated oocysts. Further joint analysis suggested that m6A modification of the majority of genes was positively correlated with mRNA expression. The mRNA relative expression and m6A level of the selected genes were confirmed by quantitative reverse transcription PCR (RT-qPCR) and MeRIP-qPCR. GO and KEGG analysis indicated that differentially m6A methylated genes (DMMGs) with significant differences in mRNA expression were closely related to processes such as regulation of gene expression, epigenetic, microtubule, autophagy-other and TOR signaling. Moreover, a total of 96 DMMGs without significant differences in mRNA expression showed significant differences at protein level. GO and pathway enrichment analysis of the 96 genes showed that RNA methylation may be involved in cell biosynthesis and metabolism of E. tenella. We firstly present a map of RNA m6A modification in E. tenella, which provides significant insights into developmental biology of E. tenella.
期刊：
Cell Biochemistry and Biophysics,2024年:1-8 ISSN：1085-9195
通讯作者：
Ji, CX
作者机构：
[Wang, Dongliang; Pan, Yanying; Liu, Jianying; Ji, Chunxiao; Chen, Tao; Zhou, Pengfei; Ji, CX] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Peoples R China.;[Wang, Dongliang; Pan, Yanying; Liu, Jianying; Ji, Chunxiao; Chen, Tao; Zhou, Pengfei; Ji, CX] Hunan Agr Univ, Hunan Engn Technol Res Ctr Vet Drugs, Changsha 410128, Peoples R China.;[Tang, Yulong] Chinese Acad Sci, Inst Subtrop Agr, Changsha 410125, Hunan, Peoples R China.
通讯机构：
[Ji, CX ] H;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Peoples R China.;Hunan Agr Univ, Hunan Engn Technol Res Ctr Vet Drugs, Changsha 410128, Peoples R China.
关键词：
DADS;MDCC-MSB-1 cells;Autophagy;MEK;ERK
摘要：
Diallyl disulfide (DADS) is effective at suppressing tumour cell growth and proliferation. This study verified the morphology and growth activity of MDCC-MSB-1 cells by using an MTT assay to detect the effect of DADS on the proliferation of MDCC-MSB-1 cells and a CCK8 assay to detect the effect of DADS on the viability and proliferation of MDCC-MSB-1 cells. We found that the viability and proliferation of MDCC-MSB-1 cells decreased with increasing DADS concentrations. MDC staining and Western blotting were used to analyse autophagy, the associated protein LC3 and the MEK/ERK pathway proteins MEK and ERK and to investigate changes in cellular autophagy based on cell morphology and molecular biology. With increasing concentrations of DADS, MDCC-MSB-1 cell autophagy increased in a gradient manner. Additionally, the conversion of the autophagy marker protein LC3-I increased with increasing drug concentrations, and the relative expression of LC3-II steadily increased, as did the expression of key protein components of the MEK/ERK signalling pathway, including P-MEK1/2 and P-ERK1/2. These results suggest that DADS induces autophagy through the MEK/ERK pathway, thereby inhibiting the proliferation of MDCC-MSB-1 cells.
作者机构：
[Huang, Chunlin] Hunan Engineering Research Center of Livestock and Poultry Health Care, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China. Electronic address: huangchunlin@stu.hunau.edu.cn;[Ou, Zhaoping] Hunan Engineering Research Center of Livestock and Poultry Health Care, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China. Electronic address: ouzhaoping@stu.hunau.edu.cn;[Kong, Li] Hunan Engineering Research Center of Livestock and Poultry Health Care, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China. Electronic address: kongli@stu.hunau.edu.cn;[Huang, You] Hunan Engineering Research Center of Livestock and Poultry Health Care, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China. Electronic address: huangyou@stu.hunau.edu.cn;[Yang, Wenjiang] Hunan Engineering Research Center of Livestock and Poultry Health Care, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China. Electronic address: yangwenjiang@stu.hunau.edu.cn
通讯机构：
[Huang, You; Wu, Jing; He, Jiayu; Yi, Jine; Yang, Mingqi; Kong, Li; Ou, Zhaoping; Yang, Wenjiang; Huang, Chunlin] H;[Xiang, Siting; Zhou, Yu] M;Hunan Engineering Research Center of Livestock and Poultry Health Care, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China. Electronic address:;Medical College, Hunan Polytechnic of Environment and Biology, Hengyang, China. Electronic address:
摘要：
T-2 toxin, a type-A trichothecene mycotoxin, exists ubiquitously in mildewed foods and feeds. Betulinic acid (BA), a pentacyclic triterpenoid derived from plants, has the effect of relieving inflammation and oxidative stress. The purpose of this study was to investigate whether BA mitigates lung impairment caused by T-2 toxin and elucidate the underlying mechanism. The results indicated that T-2 toxin triggered the inflammatory cell infiltration, morphological alterations and cell apoptosis in the lungs. It is gratifying that BA ameliorated T-2 toxin-caused lung injury. The protein expression of nuclear factor erythrocyte 2-related factor 2 (Nrf2) pathway and the markers of antioxidative capability were improved in T-2 toxin induced lung injury by BA mediated protection. Simultaneously, BA supplementation could suppress T-2 toxin-induced mitogen-activated protein kinase (MAPK)/nuclear factor-kappa B (NF-κB)-dependent inflammatory response and mitochondrial apoptotic pathway. Therefore, T-2 toxin gave rise to pulmonary toxicity, but these changes were moderated by BA administration through regulation of the Nrf2/MAPK/NF-κB pathway, which maybe offer a viable alternative for mitigating the lung impairments caused by the mycotoxin.
作者机构：
[Shurui Ren; Mingcan Wang] Hunan Key Laboratory of Traditional Chinese Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China;These authors contributed equally to this work.;College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China;[Xiaohong Zhong] College of Horticulture, Hunan Agricultural University, Changsha 410128, China;Authors to whom correspondence should be addressed.
通讯机构：
[Jianguo Zeng; Zhixing Qing] H;Hunan Key Laboratory of Traditional Chinese Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China
摘要：
Glucosinolates (GLS) in cruciferous vegetables are anti-nutritional factors. Excessive or long-term intake of GLS-containing feed is harmful to animal health and may cause kidney damage. Phenethyl isothiocyanate (PEITC) is a GLS. In this study, we investigated the inhibitory effect of PEITC on a porcine kidney (PK-15) cell line and explored the mechanism of PEITC-induced apoptosis. We found that PEITC could affect cell viability and induce cell apoptosis after incubating cells for 24h. High concentrations of PEITC can induce intracellular ROS accumulation, resulting in impaired mitochondrial function (decreased MMP, decreased ATP) and DNA damage (increased 8-OHdG), cytochrome c in mitochondria is released into the cytoplasm and activates mitochondrial pathway apoptosis-related proteins (Bcl-2 family and caspase-9, -3). Meanwhile, PEITC could induce intracellular Ca(2+) accumulation, disrupt ER homeostasis, and activate the expression levels of three ER-resident transmembrane proteins orchestrating the UPR (PERK, IRE-1α and ATF6) and ER-related proteins (GRP78 and CHOP), thereby activating ERS-pathway apoptosis-related proteins (caspase-12, -7). Our results showed that low concentration (2.5μM) of PEITC had no damaging effect on cells. In comparison, a high concentration (10μM) of PEITC could induce cell damage in porcine kidney cells and induce apoptosis in PK-15 cells via the Mitochondrial ROS-associated ERS pathway.
摘要：
Porcine epidemic diarrhea (PED) is a disease extremely harmful to pig health. Intramuscular and Houhai acupoint injections are the main immunization routes to prevent and control PED. This study aimed to evaluate the efficacy of these two routes in pregnant sows based on serum IgG, IgA, and neutralizing antibody levels. PED virus (PEDV) immunoprophylaxis with live-attenuated and inactivated vaccines was administered. The vaccinations for the intramuscular injections elevated IgG and neutralizing antibody levels more than Houhai acupoint injections at most timepoints after immunization. However, the anti-PEDV IgA antibodies induced by vaccination with the two immunization routes did not differ significantly. In conclusion, intramuscular injections are better than Houhai acupoint injections for PEDV vaccination of pregnant sows.
