作者机构：
[Zhang, Yujiao; Pu, Ling] Guizhou Acad Agr Sci, Inst Anim Husb & Vet Med, Guiyang 550005, Peoples R China.;[Wang, Jiying] Huaihua Polytech Coll, Coll Anim Sci & Technol, Huaihua 418000, Peoples R China.;[Wang, Jiying] Hunan Agr Univ, Coll Vet Med, Changsha 410000, Peoples R China.;[Zhang, Yujiao; Hu, Zhao] Guizhou Univ, Ctr R&D Fine Chem, Natl Key Lab Green Pesticide, Key Lab Green Pesticide & Agr Bioengn,Minist Educ,, Guiyang 550025, Guizhou, Peoples R China.
通讯机构：
[Zhang, YJ ; Hu, Z] G;Guizhou Acad Agr Sci, Inst Anim Husb & Vet Med, Guiyang 550005, Peoples R China.;Guizhou Univ, Ctr R&D Fine Chem, Natl Key Lab Green Pesticide, Key Lab Green Pesticide & Agr Bioengn,Minist Educ,, Guiyang 550025, Guizhou, Peoples R China.
摘要：
Efficient inactivation of bacteria in the sewage via a photocatalytic process represents a promising strategy for the efficient chemical utilization of solar energy. Herein, uniformly dispersed Fe atoms were embedded between layers of g-C(3)N(4) photocatalysts (CNFx), which were facilely prepared by thermal treatment. The optimized photocatalyst (CNF(100)) first showed excellent photoactivity for killing a variety of bacteria (93.0% for E. coli, 93.9% for Salmonella, and 96.2% for S. aureus) under visible light irradiation. The superior activity can be attributed to the formation of shallow electron traps (Fe-N(3)) that can capture excitons of excited states, which promote the charge transfer and energy transfer process of activated adsorbed molecular oxygen, respectively, forming reactive oxygen species, improving separation efficiency of photoexcited electrons and holes, and the Fe-N(3) traps can also be used as photosensitive sites to broaden the absorption range of visible light. This strategy of constructing shallow electronic traps lays a theoretical foundation for the design of new environmentally friendly and efficient water disinfectants.
摘要：
Groundwater resources are often contaminated by arsenic, which poses a serious threat to human and animal's health. Some studies have demonstrated that acute arsenic exposure could induce kidney injury because the kidney is a key target organ for toxicity, but the exact mechanism remains unclear. Hence, we investigated the effect of SIRT1-/PINK1-mediated mitophagy on NaAsO(2)-induced kidney injury in vivo and in vitro. In our study, NaAsO(2) exposure obviously induced renal tubule injury and mitochondrial dysfunction. Meanwhile, NaAsO(2) exposure could inhibit the mRNA/protein level of SIRT1 and activate the mitophagy-related mRNA/protein levels in the kidney of mice. In HK-2 cells, we also confirmed that NaAsO(2)-induced nephrotoxicity depended on the activation of mitophagy. Moreover, the activation of SIRT1 by resveratrol alleviated NaAsO(2)-induced acute kidney injury via the activation of mitophagy in vivo and in vitro. Interestingly, the inhibition of mitophagy by cyclosporin A (CsA) further exacerbated NaAsO(2)-induced nephrotoxicity and inflammation in HK-2 cells. Taken together, our study found that SIRT1-regulated PINK1-/Parkin-dependent mitophagy was implicated in NaAsO(2)-induced acute kidney injury. In addition, we confirmed that PINK1-/Parkin-dependent mitophagy played a protective role against NaAsO(2)-induced acute kidney injury. Therefore, activation of SIRT1 and mitophagy may represent a novel therapeutic target for the prevention and treatment of NaAsO(2)-induced acute renal injury.
作者机构：
[Zhang, Juzuo; Xu, Junfei] Huaihua Univ, Coll Biol & Food Engn, Key Lab Res & Utilizat Ethnomedicinal Plant Resour, Huaihua 418000, Peoples R China.;[Zhang, Juzuo; Su, Rui; Fan, Xiujun] Chinese Acad Sci, Shenzhen Inst Adv Technol, Res Ctr Reprod & Hlth Dev, Shenzhen 518055, Peoples R China.;[Yuan, Anwen; Su, Rui; Yang, Qing] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Peoples R China.
通讯机构：
[Zhang, Juzuo; Fan, Xiujun] R;Research Center for Reproduction and Health Development, Shenzhen Institutes of Advanced Technology, Chinese Academy of Science, Shenzhen 518055, China. Electronic address: xj.;Research Center for Reproduction and Health Development, Shenzhen Institutes of Advanced Technology, Chinese Academy of Science, Shenzhen 518055, China. Electronic address:
摘要：
The multifunctional molecule chondroitin sulfate proteoglycan 4 (CSPG4/NG2) plays key roles in organogenesis and tumorigenesis. However, its roles in placentation remain unclear. In this study, CSPG4 expression in human and mouse placentas was investigated through immunohistochemistry (IHC), qPCR and western blotting. The theoretical structure and function of CSPG4 were assessed using bioinformatic tools, and the functions of CSPG4 in fetal and placental development were investigated using a mouse model established by trophoblast-specific CSPG4 knockdown and a trophoblast cell line with CSPG4 knockout by lentivirus infection. The results showed that CSPG4 was mainly located in trophoblasts in both human placentas and mouse placentas, with a higher level in preeclampsia (PE) placentas than in healthy control placentas. Furthermore, there was a trend of increasing expression in mouse placentas during pregnancy. The 3D structure of CSPG4 was visualized using an M model composed of two chains, and the structure implied that CSPG4 was a multifunctional molecule containing multiple pockets with multiligand binding sites and enzyme active sites. Trophoblast-specific CSPG4 knockdown caused frequent fetal loss, and viable fetal development was restricted by poor placentation, with mice placentas having reduced weight and width. The proliferation and invasion of CSPG4-knockout trophoblasts were significantly inhibited, and as such, the molecular signaling of AKT and ERK phosphorylation was inhibited, and the expression of MMP2 and MMP9 was reduced. In summary, CSPG4 deficiency inhibited trophoblast proliferation and invasion, which was associated with AKT, ERK and MMP signaling. CSPG4 deficiency also caused pregnancy complications with poor placentation in mice.
作者机构：
[Zheng, Xiaofeng; Zhang, Yuhang] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Peoples R China.;[Zhang, Jiwen; Zhao, Dongming; Zhang, Zhenjiang] Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Harbin 150009, Peoples R China.;[Tan, Xu; Jiao, Jun; Qian, Nianchao; Zhang, Fan; Hou, Min] Tsinghua Univ, Beijing Adv Innovat Ctr Struct Biol, Beijing Frontier Res Ctr Biol Struct, Tsinghua Peking Ctr Life Sci, Beijing 100084, Peoples R China.;[Tan, Xu] Inst Hlth & Med, Hefei Comprehens Natl Sci Ctr, Hefei 230601, Peoples R China.
通讯机构：
[Tan, X ] T;[Zheng, XF ] H;[Zhao, DM ] C;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Peoples R China.;Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Harbin 150009, Peoples R China.
摘要：
African swine fever (ASF) is an acute, highly contagious and deadly viral disease in swine that jeopardizes the worldwide pig industry. Unfortunately, there are no authoritative vaccine and antiviral drug available for ASF control. African swine fever virus (ASFV) is the etiological agent of ASF. Among the ASFV proteins, p72 is the most abundant component in the virions and thus a potential target for anti-ASFV drug design. Here, we con-structed a luciferase reporter system driven by the promoter of p72, which is transcribed by the co-transfected ASFV RNA polymerase complex. Using this system, we screened over 3200 natural product compounds and obtained three potent candidates against ASFV. We further evaluated the anti-ASFV effects and proved that among the three candidates, ailanthone (AIL) inhibits the replication of ASFV at the nanomolar concentration (IC50 1/4 15 nmol/L). Our in vitro experiments indicated that the antiviral effect of AIL is associated with its inhibition of the HSP90-p23 cochaperone. Finally, we showed the antiviral activity of AIL on Zika virus and hepatitis B virus (HBV), which supports that AIL is a potential broad-spectrum antiviral agent.
作者机构：
[Li, Rong; Ma, Ping-Ping; Fu, Yi-Tian; Deng, Yuan-Ping; Liu, Guo-Hua] Hunan Agr Univ, Coll Vet Med, Res Ctr Parasites & Vectors, Changsha, Hunan, Peoples R China.;[Nie, Yu] Hunan Univ Environm & Biol, Coll Biotechnol, Hengyang, Hunan, Peoples R China.;[Wang, Wei] Univ Sunshine Coast, GeneCol Res Ctr, Anim Res Ctr, Sch Sci & Engn, Sippy Downs, Qld 4556, Australia.
通讯机构：
[Liu, GH ] H;Hunan Agr Univ, Coll Vet Med, Res Ctr Parasites & Vectors, Changsha, Hunan, Peoples R China.
摘要：
The domestic pig louse Haematopinus suis (Linnaeus, 1758) (Phthiraptera: Anoplura) is a common ectoparasite of domestic pigs, which can act as a vector of various infectious disease agents. Despite its significance, the molecular genetics, biology and systematics of H. suis from China have not been studied in detail. In the present study, the entire mitochondrial (mt) genome of H. suis isolate from China was sequenced and compared with that of H. suis isolate from Australia. We identified 37 mt genes located on nine circular mt minichromosomes, 2.9 kb-4.2 kb in size, each containing 2-8 genes and onelarge non-coding region (NCR) (1,957 bp-2,226 bp). The number of minichromosomes, gene content, and gene order in H. suis isolates from China and Australia are identical. Total sequence identity across coding regions was 96.3% between H. suis isolates from China and Australia. For the 13 protein-coding genes, sequence differences ranged from 2.8%-6.5% consistent nucleotides with amino acids. Our result is H. suis isolates from China and Australia being the same H. suis species. The present study determined the entire mt genome of H. suis from China, providing additional genetic markers for studying the molecular genetics, biology and systematics of domestic pig louse.
通讯机构：
[Liu, GH ] H;Hunan Agr Univ, Coll Vet Med, Res Ctr Parasites & Vectors, Changsha, Peoples R China.
关键词：
Entomology;Genomics;Phylogenetics
摘要：
Evidence for recombination between mitochondrial (mt) minichromosomes has been reported in sucking lice, but it is still not clear how frequent mt minichromosomal recombination occurs. We sequenced the mt genomes of the cattle louse Linognathus vituli and the goat louse L.africanus. Both Linognathus species have 10 mt minichromosomes, and seven of them have the same gene content and gene arrangement. Comparison of mt karyotypes revealed numerous inter-minichromosomal recombination events in the evolution of Linognathus species. Minichromosome merger, gene duplication and gene translocation occurred in the lineage leading to Linognathus lice. After the divergence of L.vituli and L.africanus, duplication, degeneration, deletion and translocation of genes also occurred independently in each species. Most of the recombination events in the Linognathus species occurred upstream of either cox3 or nad2, indicating these two locations were hotspots for inter-minichromosomal recombination. Our results provide an important perspective on mt genome evolution in metazoans.
期刊：
FRONTIERS IN NUTRITION,2023年10:1082250 ISSN：2296-861X
通讯作者：
Liu, Z.;Wen, L.;Wang, J.
作者机构：
[Ye, Mengke; Zou, Lirui; Li, Mengyao; Wang, Ji; Cheng, Xianyu; Wen, Lixin; Liu, Xiangyan; Qu, Jianyu] Hunan Agr Univ, Coll Vet Med, Hunan Engn Res Ctr Livestock & Poultry Hlth Care, Changsha, Peoples R China.;[Wen, Chi] Hunan Chu Ming Tea Ind Co Ltd, Changsha, Peoples R China.;[Liu, Zhonghua] Hunan Agr Univ, Coll Hort, Natl Res Ctr Engn Technol Utilizat Funct Ingredien, Key Lab Tea Sci Minist Educ, Changsha, Peoples R China.;[Wang, Ji] Hunan Agr Univ, Coll Anim Sci & Technol, Anim Nutr Genome & Germplasm Innovat Res Ctr, Changsha, Peoples R China.;[Wang, Ji] Changsha Lvye Biotechnol Co Ltd, Changsha, Peoples R China.
通讯机构：
[Wen, L.; Wang, J.] H;[Liu, Z.] K;Key Laboratory of Tea Science of Ministry of Education, China;Hunan Engineering Research Center of Livestock and Poultry Health Care, China
关键词：
compound dark tea;Gut Microbiota;Obesity;Lipid Metabolism;Hepatic Steatosis
摘要：
Dark tea is a fermented tea that plays a role in regulating the homeostasis of intestinal microorganisms. Previous studies have found that dark tea can improve obesity and has a lipid-lowering effect. In this study, green tea, Ilex latifolia Thunb (kuding tea) and Momordica grosvenori (Luo Han Guo) were added to a new compound dark tea (CDT), to improve the taste and health of this beverage. High-fat diet-fed C57BL/6J mice were treated with low- (6 mg/mL) or high- (12 mg/mL) concentrations of CDT for 18 weeks to assess their effect on lipid metabolism. Our results suggest that low- and high-concentrations of CDT could reduce body weight by 15 and 16% and by 44 and 38% of body fat, respectively, by attenuating body weight gain and fat accumulation, improving glucose tolerance, alleviating metabolic endotoxemia, and regulating the mRNA expression levels of lipid metabolism-related genes. In addition, low concentrations of CDT were able to reduce the abundance of Desulfovibrio, which is positively associated with obesity, and increase the abundance of Ruminococcus, which are negatively associated with obesity. This study demonstrates the effect of CDT on ameliorating lipid metabolism and provides new insights into the research and development of functional tea beverages.
摘要：
Camel milk, esteemed for its high nutritional value, has long been a subject of interest. However, the adulteration of camel milk with cow milk poses a significant threat to food quality and safety. Fourier-transform infrared spectroscopy (FT-MIR) has emerged as a rapid method for the detection and quantification of cow milk adulteration. Nevertheless, its effectiveness in conveniently detecting adulteration in camel milk remains to be determined. Camel milk samples were collected from Alxa League, Inner Mongolia, China, and were supplemented with varying concentrations of cow milk samples. Spectra were acquired using the FOSS FT6000 spectrometer, and a diverse set of machine learning models was employed to detect cow milk adulteration in camel milk. Our results demonstrate that the Linear Discriminant Analysis (LDA) model effectively distinguishes pure camel milk from adulterated samples, maintaining a 100% detection rate even at cow milk addition levels of 10 g/100 g. The neural network quantitative model for cow milk adulteration in camel milk exhibited a detection limit of 3.27 g/100 g and a quantification limit of 10.90 g/100 g. The quantitative model demonstrated excellent precision and accuracy within the range of 10-90 g/100 g of adulteration. This study highlights the potential of FT-MIR spectroscopy in conjunction with machine learning techniques for ensuring the authenticity and quality of camel milk, thus addressing concerns related to food integrity and consumer safety.
