作者机构：
[Yin, Jiechao; Li, Jing; Li, Guangxing; Ren, Xiaofeng] NE Agr Univ, Coll Vet Med, Harbin 150030, Peoples R China.;[Yin, Jiechao] NE Agr Univ, Coll Life Sci, Harbin 150030, Peoples R China.;[Yang, Qing] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Ren, Xiaofeng] NE Agr Univ, Coll Vet Med, 59 Mucai St, Harbin 150030, Peoples R China.
通讯机构：
[Ren, Xiaofeng] N;NE Agr Univ, Coll Vet Med, 59 Mucai St, Harbin 150030, Peoples R China.
摘要：
Avian infectious bronchitis virus (IBV), a coronavirus, causes infectious bronchitis leading to enormous economic loss in the poultry industry worldwide. Houttuynia cordata (Saururaceae) (HC) is a traditional Chinese medicine used in China. In the present study, the effect of HC on cell infection by IBV was determined using plaque assay and reverse transcription-polymerase chain reaction. The inhibitory effect of HC on IBV infection in ovo and in vivo was analysed using specific pathogen free (SPF) chicken embryos and chickens. Moreover, the effect of HC on cell apoptosis induced by IBV was investigated. Results showed that HC had more than 90% inhibition rate against IBV infection in Vero cells and chicken embryo kidney cells, and decreased more than 90% apoptotic cells caused by IBV. HC fully protected the SPF embryos, and had more than 50% protection rate in SPF chickens, against IBV challenge.
作者：
Lin, R. Q.;Wang, X. Q.;Yan, C.;He, X. H.;Cheng, T.;...
期刊：
African Journal of Microbiology Research,2011年5(24):4240-4242 ISSN：1996-0808
通讯作者：
Zhang, Y. B.
作者机构：
[Yan, C.; Wang, X. Q.; Lin, R. Q.; Xu, M. J.; Zhu, X. Q.] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Lanzhou 730046, Gansu, Peoples R China.;[Zhang, Y. B.] Sun Yat Sen Univ, Affiliated Hosp 3, Dept Emergency Med, Guangzhou 510630, Guangdong, Peoples R China.;[Wang, Y. N.; Wang, X. Q.; Lin, R. Q.; Cheng, T.; He, X. H.; Yuan, Z. G.] S China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China.;[Yan, C.] Xuzhou Med Coll, Dept Pathogen Biol & Immunol, Xuzhou 221004, Jiangsu, Peoples R China.;[Zhu, X. Q.] Heilongjiang Bayi Agr Univ, Coll Anim Sci & Vet Med, Daqing 163319, Heilongjiang Pr, Peoples R China.
通讯机构：
[Zhang, Y. B.] S;Sun Yat Sen Univ, Affiliated Hosp 3, Dept Emergency Med, Guangzhou 510630, Guangdong, Peoples R China.
关键词：
Chlamydophila psittaci is widely distributed in a number of birds and humans throughout the world;but little is known of the prevalence of C. psittaci in birds in China. In the present study;sera from 240 chickens;200 ducks;200 geese and 200 pigeons in China’s southern Guangdong Province were assayed for Chlamydophila antibodies by indirect hemagglutination assay (IHA). The specific antibodies were found in sera of 17.92% of 240 chickens;6% of 200 ducks;22% of 200 geese and 17% of 200 pigeons. Statistical analysis showed that the seroprevalence of C. psittaci in ducks was significantly lower than that in chickens;geese and pigeons;respectively (P < 0.05). The seroprevalence in free-range chickens was 10.83%;and it was 25% in caged chickens;and the difference was statistically significant (P < 0.05). Among different age groups of pigeons;the difference in seroprevalence of C. psittaci between adult pigeons and adolescent pigeons was significant (P < 0.05). This is the first time that the seroprevalence of Chlamydophila infection in domestic birds in Southern China was reported;and the data showed that the prevalence of Chlamydophila infection in domestic birds is high;indicating that the domestic birds may be an important source for human infection by C. psittaci in Southern China.
摘要：
Chlamydophila psittaci is widely distributed in a number of birds and humans throughout the world, but little is known of the prevalence of C. psittaci in birds in China. In the present study, sera from 240 chickens, 200 ducks, 200 geese and 200 pigeons in China's southern Guangdong Province were assayed for Chlamydophila antibodies by indirect hemagglutination assay (IHA). The specific antibodies were found in sera of 17.92% of 240 chickens, 6% of 200 ducks, 22% of 200 geese and 17% of 200 pigeons. Statistical analysis showed that the seroprevalence of C. psittaci in ducks was significantly lower than that in chickens, geese and pigeons, respectively (P < 0.05). The seroprevalence in free-range chickens was 10.83%, and it was 25% in caged chickens, and the difference was statistically significant (P < 0.05). Among different age groups of pigeons, the difference in seroprevalence of C. psittaci between adult pigeons and adolescent pigeons was significant (P < 0.05). This is the first time that the seroprevalence of Chlamydophila infection in domestic birds in Southern China was reported, and the data showed that the prevalence of Chlamydophila infection in domestic birds is high, indicating that the domestic birds may be an important source for human infection by C. psittaci in Southern China.
期刊：
Toxicology and Applied Pharmacology,2011年252(3):281-288 ISSN：0041-008X
通讯作者：
Yuan, Zonghui
作者机构：
[Wang, Yulian; Li, Tingting; Wang, Xu; Liu, Zhaoying; Liu, Yu; Ihsan, Awais; Yu, Huan; Yuan, Zonghui; Zhang, Hongfei; Yang, Chunhui; Huang, Xianju] Huazhong Agr Univ, Natl Reference Lab Vet Drug Residues, Wuhan 430070, Peoples R China.;[Yuan, Zonghui] Huazhong Agr Univ, Natl Reference Lab Vet Drug Residues HZAU, MOA Key Lab Food Safety Evaluat, Wuhan 430070, Peoples R China.;[Liu, Zhaoying] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Huang, Xianju] S Cent Univ Nationalities, Coll Pharm, Wuhan 430074, Peoples R China.
通讯机构：
[Yuan, Zonghui] H;Huazhong Agr Univ, Natl Reference Lab Vet Drug Residues HZAU, MOA Key Lab Food Safety Evaluat, Wuhan 430070, Peoples R China.
关键词：
DNA damage;Mequindox;Oxidative stress;Quinoxaline;Rats;Testis;Testosterone;8-hydroxydeoxyguanosine (8-OHdG)
摘要：
Mequindox (MEQ) is a synthetic antimicrobial chemical of quinoxaline 1, 4-dioxide group. This study was designed to investigate the hypothesis that MEQ exerts testicular toxicity by causing oxidative stress and steroidal gene expression profiles and determine mechanism of MEQ testicular toxicity. In this study, adult male Wistar rats were fed with MEQ for 180 days at five different doses as 0, 25, 55, 110 and 275 mg/kg, respectively. In comparison to control, superoxide dismutase (SOD), reduced glutathione (GSH) and 8-hydroxydeoxyguanosine (8-OHdG) levels were elevated at 110 and 275 mg/kg MEQ whereas the malondialdehyde (MDA) level was slightly increase at only 275 mg/kg. Furthermore, in LC/MS-IT-TOF analysis, one metabolite 2-isoethanol 4-desoxymequindox (M11) was found in the testis. There was significant decrease in body weight, testicular weight and testosterone at 275 mg/kg, serum follicular stimulating hormone (FSH) at 110 and 275 mg/kg, while lutinizing hormone (LH) levels were elevated at 110 mg/kg. Moreover, histopathology of testis exhibited germ cell depletion, contraction of seminiferous tubules and disorganization of the tubular contents of testis. Compared with control, mRNA expression of StAR, P450scc and 17 beta-HSD in testis was significantly decreased after exposure of 275 mg/kg MEQ while AR and 3 beta-HSD mRNA expression were significantly elevated at the 110 mg/kg MEQ group. Taken together, our findings provide the first and direct evidence in vivo for the formation of free radicals during the MEQ metabolism through N -> O group reduction, which may have implications to understand the possible mechanism of male infertility related to quinoxaline derivatives. (C) 2011 Elsevier Inc. All rights reserved.
通讯机构：
[Yu, Xing-Long] H;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
关键词：
Defective interfering RNA;Porcine reproductive and respiratory syndrome virus
摘要：
In the present study, seven new defective interfering (DI) RNA species of porcine reproductive and respiratory syndrome virus (PRRSV) were identified. RT-PCR, Northern blot and sequence analyses indicated that these DI RNA specie have deletions of 8513-9176 nucleotides located between Nsp1/Nsp2 and Nsp10. Compared with the previous DI RNAs of PRRSV reported, they have three distinct characteristics: much smaller deletion sizes; different nucleotide repeats (2-12 nt) used in the junction sites and in-frame deletions. The results further suggested that the similarity-assisted RNA recombination may be the main cause of generation of DI RNAs in PRRSV and probably in other arteriviruses. (C) 2011 Elsevier B.V. All rights reserved.
