摘要：
1发病情况2012年7月，湖南邵阳市某规模化猪场产房某单元角落的两窝仔猪在产后第4天发病，病猪眼眶周围、耳及腹下出现红斑，伴有淡黄色小水泡生成，扎堆，体温40℃左右，有4头在出现症状后第2天因严重脱水而死亡；3 d 后病情蔓延至整个产房单元，感染的皮肤因渗出液与产床上的脏物混合而形成厚厚的、褐色、油腻并恶臭的痂皮；随时间的发展，陆续有病仔猪因脱水、营养缺乏及败血而死亡，耐过猪消瘦、体弱，饲料报酬低。2012年7月12—21日，该产房单元产24窝共226头活壮仔，断奶前共发病93头，大部分为5~20日龄，死亡49头，发病率41.15%，病死率高达52.69%。
摘要：
High-titer serologically detected male (SDM) antibody fragments are essential for specific binding to the SDM antigen and promoting its application. The A8 clone previously obtained from an original phage antibody library was further affinity-matured by light- and high-chain shuffling respectively, to generate the end product B9 clone. The binding capacity of B9 phage Fabs to male splenocytes doubled the value of its parental A8 clone (determined using ELISA). Based on immunofluorescent staining, B9-Fabs mainly bound to the surface antigen of male splenocytes and recognized testicular cells. The resulting B9-Fabs detected a single protein (approximately 40 kDa determined using Western blot analysis of male splenocytes and testis); its high SDM antigen binding ability might have been because of mutation sites and varied lengths of the amino acid sequences in the complementarity determining regions-3 of the kappa and Fd chains. The new recombinant clones of Fab that were phage-enhanced using chain shuffling were candidate molecules for investigating molecular mechanisms of SDM antigens specific binding and applications. (C) 2013 Elsevier Inc. All rights reserved.
作者：
Deng Zhibang*;Gong Qianlong;Wang Naidong;Yuan Anwen;Yu Xing Long
期刊：
TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES,2013年37(4):475-478 ISSN：1300-0128
通讯作者：
Deng Zhibang
作者机构：
[Gong Qianlong; Yu Xing Long; Deng Zhibang; Wang Naidong; Yuan Anwen] Hunan Agr Univ, Coll Vet Med, Changsha, Hunan, Peoples R China.
通讯机构：
[Deng Zhibang] H;Hunan Agr Univ, Coll Vet Med, Changsha, Hunan, Peoples R China.
关键词：
PCV2b DNA-positive semen;artificial insemination;PCV2b transmission to fetuses;reproductive failure;Kunming mice
摘要：
In order to investigate whether porcine circovirus 2b (PCV2b) can be replicated in the spermatids of mice and whether semen from PCV2b-infected male mice can induce reproductive failure in female mice, 10 male Kunming mice were intraperitoneally inoculated with PCV2b, and 10 female Kunming mice were artificial inseminated with semen from the PCV2b-inoculated male mice. Semen from inoculated male mice and newborn mice or dead fetuses from inseminated female mice were tested for PCV2b by polymerase chain reaction, transmission electron microscopy, and immunofluorescent histochemistry. The results showed that PCV2b was capable of being replicated in spermatids in Kunming mice and could be transmitted from semen to fetuses by artificial insemination, causing reproductive failure in pregnant Kunming mice and PCV2b vertical infection to the newborn mice.
作者机构：
[Gong, Qian Long; Xue, Li Qun; Wang, Nai Dong; Yu, Xing Long; Deng, Zhi Bang; Luo, Wei; Yuan, An Wen] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Xue, Li Qun] H;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
关键词：
Porcine circovirus;PCV2b transmission;contact and vertical infection;Kunming mice
摘要：
To investigate porcine circovirus type 2b (PCV2b) transmission by contact and vertical infection in Kunming mice (an outbred mouse stock deriving from Swiss albino mice with a high ratio of gene heterozygosis), four mice in cage 6 were inoculated with PCV2b and 25 mice without any treatment were placed into cages 1 to 5 (five mice in each cage). Seven days after being infected, the PCV2-binoculated mice were co-mingled with non-inoculated mice from cages 1 to 5 successively at 7, 14, 21, 28 and 35 days post infection (dpi), respectively, for 3 days. In addition, eleven pregnant mice were injected with PCV2b. Samples were collected from non-inoculated mice and three newborn mice from each litter for PCV2b detection by polymerase chain reaction (PCR) and immunohistochemistry (IHC). The PCR results showed that PCV2b transmission rate among mice in cages 1, 2, 3, 4 and 5 was 0/5, 2/5, 5/5, 5/5 and 1/5, respectively. PCV2b antigen signals generally appeared in most organs of the non-inoculated mice in which viruses were detected by PCR. PCV2b DNA was also detected in newborn mice of PCV2b-infected litters, and viral antigen signals were observed in their organs as well. PCV2b was transmitted in Kunming mice by contact, and it also caused vertical infection through the placenta.
