作者： Liu, Yan-Chun;Lin, Li;Cheng, Pi;Sun, Zhi-Liang;Wu, Yong;...

期刊： Fitoterapia,2017年121:94-105 ISSN：1971-551X

通讯作者： Liu, Zhao-Ying

作者机构： [Wu, Yong; Sun, Zhi-Liang; Liu, Zhao-Ying; Liu, Yan-Chun; Lin, Li] Hunan Agr Univ, Hunan Engn Res Ctr Vet Drug, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhao-Ying; Lin, Li; Cheng, Pi] Hunan Agr Univ, Natl & Local Union Engn Res Ctr Vet Herbal Med Re, Changsha 410128, Hunan, Peoples R China.;[Wu, Yong; Sun, Zhi-Liang; Liu, Zhao-Ying] Hunan Agr Univ, Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhao-Ying] Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Liu, Zhao-Ying] H;Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.

关键词： 14-Acetoxy-15-hydroxygelsenicine (PubChem CID:102185548);14-Acetoxygelsenicine (PubChem CID:102185547);14-Hydroxygelsenicine (PubChem CID:14217347);14-hydroxygelsemicine (PubChem CID:22217388);19R-Hydroxydihydrogelsemine (PubChem CID:101601182);7-Deoxygelsemide or 9-Deoxygelsemide (PubChem CID:101413879 or 46176622);Alkaloids, fingerprint;Chemical constituents;Dehydrokoumidine (PubChem CID:119077162);Ferulic acid (PubChem CID:445858);GS-2 (PubChem CID:12070888);GSIR-1 (PubChem CID:11105986);Gallic acid (PubChem CID:370);Gelsemicine (PubChem CID: 54624 28);Gelsemine (PubChem CID:5390854);Gelsemiol (PubChem CID:10375526);Gelsemiol-3-glucoside (PubChem CID:101098709);Gelsemium elegans;Gelsenicine (PubChem CID: 21123652);Gelseoxazolidinine (PubChem CID:102297300);Gelsevirine (PubChem CID:14217344);HPLC;Humantenine (PubChem CID:44593672);11-hydroxyhumantenine (PubChem CID:5318224);Koumidine (PubChem CID:44584550);Koumine (PubChem CID:91895267);Mass spectrometry;Pyrogallol (PubChem CID:105);Semperoside (PubChem CID:11439812);Sempervirine (PubChem CID:168919)

摘要： Gelsemium elegans, which is a genus of the family Loganiaease, is commonly used as a traditional medicine for promoting animal growth and treating rheumatoid arthritis pain and neuropathic pain, among others. In this study, we first established a valid high-performance liquid chromatography (HPLC) method for the fingerprint analysis of Gelsemium elegans samples. Then, the comprehensive detection of chemical constituents from the samples was performed using HPLC coupled with quadrupole-time-of-flight mass spectrometry. The similarity evaluation results showed that location and area differences influenced the quality of the samples. An efficient strategy for the rapid targeted identification of chemical components was matching with a developed Gelsemium database. As a result, the accurate elemental compositions and known structures of compounds are found as hits. This process facilitated the structural identification of compounds combined with the accurate mass measurement of product ions and fragmentation behaviors. Consequently, 41 components including six alkaloids and non-alkaloids were systematically identified from Gelsemium elegans. The results showed that at least seven relatively major components existing in Gelsemium elegans may be useful for its quality control. The present analytical method combined with the developed Gelsemium database was shown to be a useful tool for investigating the chemical components of Gelsemium products. © 2017 Elsevier B.V.

语种： 英文

作者： 刘兆颖;陈敏

期刊： 教育教学论坛,2017年(46):92-94 ISSN：1674-9324

作者机构： 湖南农业大学动物医学院,长沙,410128;[陈敏; 刘兆颖] 湖南农业大学

关键词： 湖南农业大学;卓越农林人才;人才培养

摘要： 农林改革的教育模式“卓越农林人才”是充分地将理论和实践相结合,是一种更能适应新时期社会发展的教育模式。本文以湖南农业大学动物医学院15级学生作为调研对象,对设置卓越班进行问卷调查。利用调查结果比较分析卓越班和普通班学生对教育改革模式的看法,同时介绍学院为提高人才培养质量做的各种改革。望通过“卓越农林人才”教育模式来提升我校大学生的创新水平,为改革人才培养质量方式提高途径。

语种： 中文

作者： 孙卓;伍勇;王楠;吴巧月;邓海冰;...

期刊： 中兽医医药杂志,2017年36(4):25-27 ISSN：1000-6354

作者机构： [孙卓; 伍勇] 湖南农业大学东方科技学院;湖南农业大学动物医学院;[刘兆颖] 湖南省兽药工程技术研究中心;[吴巧月; 杨玉娟; 邓海冰; 孟奎宇; 王楠] 湖南农业大学

关键词： 血根碱;小鼠;肝微粒体;细胞色素P450;细胞色素b5

摘要： 研究血根碱对小鼠肝细胞色素P450与b5的影响,为血根碱的兽医临床应用提供实验依据。将血根碱按高剂量[21 mg/（kg·d）]、中剂量[10.5 mg/（kg·d）]、低剂量[2.1 mg/（kg·d）]给小鼠给连续灌胃给药7 d后,采用考马斯亮蓝法测定小鼠肝微粒体蛋白含量;紫外-可见光分光光度法测定细胞色素P450和细胞色素b5的含量。结果显示,与空白组相比,高、中剂量组小鼠肝微粒体蛋白含量、细胞色素P450和b5活性降低（P〈0.05）,而低剂量组无显著变化（P〉0.05）。结果表明,高、中剂量组血根碱对小鼠肝细胞色素P450和b5有抑制作用。

语种： 中文

作者： 代佳娣;李玉娟;张卓亿;孙志良;刘兆颖

作者机构： [代佳娣; 李玉娟; 张卓亿; 孙志良; 刘兆颖] 湖南农业大学动物医学院

会议名称： 中国毒理学会兽医毒理学委员会与中国畜牧兽医学会兽医食品卫生学分会联合学术研讨会暨中国毒理学会兽医毒理学委员会第5次全国会员代表大会

会议时间： 2017-09-22

会议地点： 中国北京

关键词： 钩吻;人肝癌细胞;代谢;细胞凋亡

摘要： 【目的】本试验通过比较三种不同的钩吻粗提物(钩吻醇提物、水提物以及酸水提取物)对体外培养的人肝癌细胞(HepG2)的毒性作用,并通过加入肝S9与NADPH和细胞的共孵育来进一步探讨三种粗提物对细胞毒性的影响,以期研究三种钩吻提取物对体外培养的细胞毒性作用的差异,以及其是否存在代谢活化或者代谢失活。为今后更深入地研究钩吻的毒性与代谢奠定基础。【方法】用不同浓度的三种钩吻粗提物孵育HepG2细胞24h

语种： 中文

作者： 李玉娟;代佳娣;张大生;孙志良;刘兆颖

期刊： 中国抗生素杂志,2016年41(5):388-395 ISSN：1001-8689

作者机构： [李玉娟; 代佳娣; 张大生; 孙志良; 刘兆颖] 湖南农业大学动物医学院, 湖南省兽药工程技术研究中心, 长沙, 410128

关键词： 达氟沙星;氧化应激;肾毒性;细胞周期

摘要： 目的探讨达氟沙星诱导LLC-PK1细胞氧化应激后细胞损伤的分子机制。方法不同浓度的达氟沙星处理LLC-PK1细胞后,通过WST-1法检测细胞增殖抑制情况,DHE、Amplex UltraRed法分别检测超氧阴离子自由基(O_2~(·-))、H_2O_2含量, Rhodamine123法检测线粒体膜电位,流式细胞仪检测分析细胞周期及RT-PCR法检测细胞周期相关基因表达含量的变化。结果达氟沙星呈浓度依赖性的抑制LLC-PK1细胞的增殖、升高细胞内O_2~(·`)、H_2O_2含量、诱导细胞周期阻滞,但线粒体膜电位没有显著性下降。100μmol/L浓度达氟沙星处理LLC-PK1细胞后细胞出现G2期周期阻滞,ROS升高含量在自身抗氧化系统修复范围内,细胞并没有出现明显损伤;而浓度达到400μmol/L时细胞发生氧化应激出现可逆性的G1期细胞阻滞,此时细胞周期素抑制蛋白p53、p21、p27mRNA表达显著升高,LLC-PK1细胞对氧化应激造成的损伤进行修复。结论400μmOl/L浓度范围内的达氟沙星处理LLC-PK1细胞24h所致的氧化应激诱导细胞周期阻滞,细胞损伤在自身修复范围之内并未导致细胞凋亡。

语种： 中文

作者： Wang, Xu;Martinez, Maria-Aranzazu;Cheng, Guyue;Liu, Zhaoying;Huang, Lingli;...

期刊： Drug Metabolism Reviews,2016年48(2):159-182 ISSN：0360-2532

通讯作者： Anadon, Arturo

作者机构： [Wang, Xu; Yuan, Zonghui] Natl Reference Lab Vet Drug Residues HZAU, Wuhan, Hubei, Peoples R China.;[Wang, Xu; Yuan, Zonghui] MAO Key Lab Detect Vet Drug Residues, Wuhan, Hubei, Peoples R China.;[Anadon, Arturo; Martinez, Maria-Aranzazu; Martinez-Larranaga, Maria-Rosa] Univ Complutense Madrid, Dept Toxicol & Pharmacol, Fac Vet Med, E-28040 Madrid, Spain.;[Cheng, Guyue; Huang, Lingli; Yuan, Zonghui; Dai, Menghong; Chen, Dongmei] Huazhong Agr Univ, MOA Lab Risk Assessment Qual & Safety Livestock &, Wuhan, Hubei, Peoples R China.;[Liu, Zhaoying] Hunan Agr Univ, Coll Vet Med, Hunan Engn Res Ctr Vet Drugs, Changsha, Hunan, Peoples R China.

通讯机构： [Anadon, Arturo] U;Univ Complutense Madrid, Dept Toxicol & Pharmacol, Fac Vet Med, E-28040 Madrid, Spain.

关键词： *Quinoxaline;*antioxidants;*carbadox;*cyadox;*mequindox;*olaquindox;*oxidative stress;*quinocetone;*reactive oxygen species

摘要： Quinoxaline 1,4-dioxide derivatives (QdNOs) have been widely used as growth promoters and antibacterial agents. Carbadox (CBX), olaquindox (OLA), quinocetone (QCT), cyadox (CYA) and mequindox (MEQ) are the classical members of QdNOs. Some members of QdNOs are known to cause a variety of toxic effects. To date, however, almost no review has addressed the toxicity and metabolism of QdNOs in relation to oxidative stress. This review focused on the research progress associated with oxidative stress as a plausible mechanism for QdNO-induced toxicity and metabolism. The present review documented that the studies were performed over the past 10 years to interpret the generation of reactive oxygen species (ROS) and oxidative stress as the results of QdNO treatment and have correlated them with various types of QdNO toxicity, suggesting that oxidative stress plays critical roles in their toxicities. The major metabolic pathways of QdNOs are N→O group reduction and hydroxylation. Xanthine oxidoreductase (XOR), aldehyde oxidase (SsAOX1), carbonyl reductase (CBR1) and cytochrome P450 (CYP) enzymes were involved in the QdNOs metabolism. Further understanding the role of oxidative stress in QdNOs-induced toxicity will throw new light onto the use of antioxidants and scavengers of ROS as well as onto the blind spots of metabolism and the metabolizing enzymes of QdNOs. The present review might contribute to revealing the QdNOs toxicity, protecting against oxidative damage and helping to improve the rational use of concurrent drugs, while developing novel QdNO compounds with more efficient potentials and less toxic effects. © 2016 Informa UK Limited, trading as Taylor & Francis Group.

语种： 英文

作者： 代佳娣;张卓亿;孙志良;刘兆颖

期刊： 中兽医医药杂志,2016年35(2):30-33 ISSN：1000-6354

作者机构： 湖南农业大学动物医学院 湖南省植物功能协同创新中心,湖南长沙,410128;[代佳娣; 刘兆颖; 张卓亿; 孙志良] 湖南农业大学

关键词： 钩吻;药理作用;研究进展

摘要： 钩吻（Gelsmium elegans）曾被用来治疗皮肤疾病等,其药效明显,但内服有剧毒,使其应用受到限制。随着近代研究的深入,钩吻更多的药理作用也被进一步挖掘。本文对钩吻在抗肿瘤、免疫、抗炎镇痛、散瞳等方面的作用进行综述,为开发适合临床应用的药物提供参考。

语种： 中文

作者： Li, Quan;Liu, Zhaoying;Kolli, Shamalatha;Wetz, Karen;Griffith, Niesha;...

期刊： AMERICAN JOURNAL OF HEALTH-SYSTEM PHARMACY,2016年73(17):1331-1337 ISSN：1079-2082

通讯作者： Poi, Ming J.

作者机构： [Li, Quan; Griffith, Niesha; Poi, Ming J.] Ohio State Univ, Wexner Med Ctr, James Comprehens Canc Hosp, Dept Pharm, Columbus, OH 43210 USA.;[Li, Quan; Griffith, Niesha; Poi, Ming J.] Ohio State Univ, Wexner Med Ctr, Richard Solove Res Inst, Columbus, OH 43210 USA.;[Liu, Zhaoying] Hunan Agr Univ, Coll Vet Med, Changsha, Hunan, Peoples R China.;[Kolli, Shamalatha] Ohio State Univ, Coll Pharm, 500 W 12th Ave, Columbus, OH 43210 USA.;[Wetz, Karen] Ohio State Univ, Dept Pharm, Wexner Med Ctr, Columbus, OH 43210 USA.

通讯机构： [Poi, Ming J.] O;Ohio State Univ, Wexner Med Ctr, James Comprehens Canc Hosp, Dept Pharm, Columbus, OH 43210 USA.;Ohio State Univ, Wexner Med Ctr, Richard Solove Res Inst, Columbus, OH 43210 USA.;Ohio State Univ, Coll Pharm, Div Pharm Practice & Sci, 500 W 12th Ave, Columbus, OH 43210 USA.

摘要： Purpose: The stability of extemporaneously prepared erlotinib, lapatinib, and imatinib oral liquid dosage forms using two commercially available vehicles when stored at 4 and 25 °C was evaluated. Methods: Three batches of extemporaneous oral suspensions were prepared for each drug. Erlotinib and lapatinib tablets were crushed and mixed in a 1:1 mixture of Ora-Plus:Ora-Sweet solution to yield 10- and 50-mg/mL suspensions, respectively. Imatinib tablets were crushed and mixed in Ora-Sweet solution to yield a 40-mg/mL suspension. Suspensions were stored in amber plastic bottles, and samples from each bottle were obtained on days 0, 1, 3, 7, 14, and 28. Results: Erlotinib 10-mg/mL and lapatinib 50-mg/mL oral suspensions in a 1:1 mixture of Ora-Plus and Ora-Sweet retained at least 90% of their initial concentration throughout the 28-day study when stored at 25 °C. Visual inspection revealed notable viscosity changes in the erlotinib and lapatinib suspensions stored at 4 °C for 7 days and beyond. The viscosity of these preparations increased with time and was particularly evident with the erlotinib suspension, which exhibited a puddinglike texture. Imatinib 40-mg/mL oral suspension in Ora-Sweet appeared stable for up to 14 days when stored at both 25 and 4 °C. Conclusion: Erlotinib 10-mg/mL and lapatinib 50-mg/mL oral suspensions prepared from commercially available tablets were stable for at least 28 days when prepared in a 1:1 mixture of Ora-Plus:Ora-Sweet at 25 °C. Imatinib 40-mg/mL oral suspension prepared from commercially available tablets was stable for up to 14 days when prepared in Ora-Sweet and stored at 25 and 4 °C. Copyright © 2016, American Society of Health-System Pharmacists, Inc. All rights reserved.

语种： 英文

作者： Huang, Ya-Jun;Xiao, Sa;Sun, Zhi-Liang;Zeng, Jian-Guo;Liu, Yi-Song;...

期刊： Rapid Communications in Mass Spectrometry,2016年30(13):1549-1559 ISSN：0951-4198

通讯作者： Liu, Zhao-Ying

作者机构： [Sun, Zhi-Liang; Zeng, Jian-Guo; Liu, Zhao-Ying; Liu, Yi-Song; Xiao, Sa; Huang, Ya-Jun] Hunan Agr Univ, Coll Vet Med, Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha 410128, Hunan, Peoples R China.;[Sun, Zhi-Liang; Liu, Zhao-Ying; Xiao, Sa; Huang, Ya-Jun] Hunan Agr Univ, Coll Vet Med, Hunan Engn Res Ctr Vet Drug, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhao-Ying] Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Liu, Zhao-Ying] H;Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.

摘要： RATIONALE: Allocryptopine (AL) and protopine (PR) have been extensively studied because of their anti-parasitic, anti-arrhythmic, anti-thrombotic, anti-inflammatory and anti-bacterial activity. However, limited information on the pharmacokinetics and metabolism of AL and PR has been reported. Therefore, the purpose of the present study was to investigate the in vitro metabolism of AL and PR in rat liver S9 using a rapid and accurate high-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry (HPLC/QqTOFMS) method. METHODS: The incubation mixture was processed with 15% trichloroacetic acid (TCA). Multiple scans of AL and PR metabolites and accurate mass measurements were automatically performed simultaneously through data-dependent acquisition in only a 30-min analysis. The structural elucidations of these metabolites were performed by comparing their changes in accurate molecular masses and product ions with those of the precursor ion or metabolite. RESULTS: Eight and five metabolites of AL and PR were identified in rat liver S9, respectively. Among these metabolites, seven and two metabolites of AL and PR were identified in the first time, respectively. The demethylenation of the 2,3-methylenedioxy, the demethylation of the 9,10-vicinal methoxyl group and the 2,3-methylenedioxy group were the main metabolic pathways of AL and PR in liver S9, respectively. In addition, the cleavage of the methylenedioxy group of the drugs and subsequent methylation or O-demethylation were also the common metabolic pathways of drugs in liver S9. In addition, the hydroxylation reaction was also the metabolic pathway of AL. CONCLUSIONS: This was the first investigation of in vitro metabolism of AL and PR in rat liver S9. The detailed structural elucidations of AL and PR metabolites were performed using a rapid and accurate HPLC/QqTOFMS method. The metabolic pathways of AL and PR in rat were tentatively proposed based on these characterized metabolites and early reports. Copyright (c) 2016 John Wiley & Sons, Ltd.

语种： 英文

作者： 杨迪;刘兆颖;柳亦松;曾建国

期刊： 饲料博览,2016年(3):11-14 ISSN：1001-0084

作者机构： 湖南农业大学兽用中药资源与中兽药创制国家地方联合工程研究中心,长沙410128;湖南农业大学动物医学院,长沙410128;湖南农业大学湖南省植物功能成分利用协同创新中心,长沙410128;[曾建国; 柳亦松; 刘兆颖; 杨迪] 湖南农业大学

关键词： 原阿片碱;别隐品碱;博落回;药代动力学

摘要： 原阿片碱和α-别隐品碱是普罗托品类生物碱中重要的两种生物碱,具有抗菌、消炎等多种生物活性,因其还具有抗寄生虫生物活性,将是一个兽药和药物饲料添加剂开发的新选择。近年来,兽药药代动力学研究逐渐开始采用高效液相色谱法和高效液相色谱串联质谱法。文章综述了近十几年来这两种生物碱在大鼠、兔、比格犬等动物的体内药代动力学研究进展。

语种： 中文

作者： 刘艳纯;孙志良;刘兆颖

期刊： 中国兽药杂志,2016年50(10):55-64 ISSN：1002-1280

作者机构： [刘艳纯; 刘兆颖; 孙志良] 湖南省植物功能协同创新中心;湖南农业大学动物医学院

关键词： 钩吻;非生物碱;环烯醚萜;化学成分

摘要： 钩吻在传统中药中常用来治疗类风湿性关节炎疼痛,神经性疼痛,痉挛,皮肤溃疡和癌症等,其主要活性成分是生物碱。国内外研究表明钩吻中非生物碱成分也具有很强的药理活性。本文对钩吻中非生物碱的化学成分进行全面综述,详细总结了国内外报道有关环烯醚萜、油脂、三萜,酚酸和甾体等非生物碱化学成分的结构与药理作用,为钩吻非生物碱的化学成分与药理作用研究提供重要的依据。

语种： 中文

作者： 谢小伟;孙志良;李元义;刘兆颖;陈小军

期刊： 中兽医医药杂志,2016年35(4):8-11 ISSN：1000-6354

作者机构： 湖南农业大学动物医学院,湖南长沙,410128;[谢小伟; 李元义; 刘兆颖; 陈小军; 孙志良] 湖南农业大学

关键词： 猪链球菌2型;生长曲线

摘要： 为精确测定猪链球菌2型的半数致死量,通过对猪链球菌2型菌株进行液体培养,采用分光光度计测定茵液的实时OD600值,制作细菌的生长曲线,并确定OD600值与茵液稀释倍数的关系、猪链球菌对BALB/c小鼠的半数致死量.结果猪链球菌2型用THB液体培养基培养2h达到对数生长期,培养至5h的茵液OD600值为1.51,此时对应的活茵浓度为5.3×108 CFU/mL;猪链球菌2型对BALB/c小鼠的LD50为1.116×108 CFU,95％置信区间为9.429×108-1.31 1×108 CFU.

语种： 中文

作者： 肖洒;伍勇;黄亚军;孙志良;刘兆颖

期刊： 动物医学进展,2016年37(6):72-76 ISSN：1007-5038

作者机构： 湖南省植物功能协同创新中心，湖南农业大学动物医学院，湖南长沙 410128;[刘兆颖; 黄亚军; 肖洒; 孙志良; 伍勇] 湖南农业大学

关键词： 钩吻;粗提物;含量测定

摘要： 钩吻为马钱科钩吻属常绿藤本植物,具有抗肿瘤、免疫调节、促生长等功效,研究表明其主要活性和毒性成分均为吲哚类生物碱。钩吻的药效剂量与中毒剂量十分接近,且致死量低,为给钩吻广泛的临床应用提供理论依据,分别采用酸性染料比色法、苯酚-硫酸法、考马斯亮蓝法对3种不同溶剂工业化提取的钩吻粗取物中生物碱、多糖、蛋白质进行含量测定。结果显示,钩吻醇提（950 mL/L乙醇）、酸水提（5 mL/L H2SO4）、水提物中生物总碱含量分别为64.4g/L、21.5g/L和5.7g/L,糖含量分别为264.1g/L、89.6g/L和9.3g/L,蛋白含量分别为44.7g/L、12.7g/L和34.6g/L。

语种： 中文

作者： Liu, Zhao-Ying;Yang, Kun;Chen, Fu-Hua;Long, Xue-Ming;Deng, Yun-Bo;...

期刊： Food Analytical Methods,2015年8(2):352-362 ISSN：1936-9751

通讯作者： Sun, Zhi-Liang

作者机构： [Sun, Zhi-Liang; Yang, Kun; Liu, Zhao-Ying] Hunan Agr Univ, Coll Vet Med, Hunan Engn Res Ctr Vet Drug, Changsha 410128, Hunan, Peoples R China.;[Kuang, Guang-Wei; Chen, Fu-Hua; Deng, Yun-Bo; Long, Xue-Ming] Hunan Prov Inst Anim Drug & Feed Supervis, Changsha 410006, Hunan, Peoples R China.

通讯机构： [Sun, Zhi-Liang] H;Hunan Agr Univ, Coll Vet Med, Hunan Engn Res Ctr Vet Drug, Changsha 410128, Hunan, Peoples R China.

关键词： Animal tissues;Flunixin;Liquid chromatography–tandem mass spectrometry;Residue;Validation method

摘要： Flunixin is approved for use in veterinary medicine as a nonsteroidal anti-inflammatory agent. There is a need for the control of residues and development of methods to monitor its compliance with legislation. However, few methods have been reported for the analysis of flunixin in the animal liver, kidney, and fat. Therefore, the aim of this work was to develop a relatively rapid sample preparation and sensitive using liquid chromatography–tandem mass (LC-MS/MS) confirmatory method to detect flunixin residues in the liver, kidney, muscle, and fat of swine and chicken. After acid hydrolysis, the sample was extracted with ethyl acetate. The extract was finally evaporated to dryness and reconstituted in a water/methanol mixture, and determination was carried out by LC-MS/MS. Flunixin was detected using positive electrospray ionization in selected reaction monitoring (SRM). Estimated limit of quantification of the method was 0.5, 0.5, 0.25, and 0.05 μg/kg for the liver, kidney, muscle, and fat, respectively. The method was validated in animal tissues in terms of selectivity, linearity, trueness, precision, decision limit (CCα), and detection capability (CCβ). All the trueness values fell within a range between 73.6 and 84.8 %. Precision values for all levels of concentration tested showed excellent relative standard deviation (RSD <15 %). The CCαand CCβvalues have been established for each tissue. A relatively rapid and sensitive LC-MS/MS method for the quantitative determination of flunixin in different animal tissues was developed and validated. The method is suitable for monitoring the flunixin residues in animal tissues. ©2014, Springer Science+Business Media New York.

语种： 英文

作者： Zhang, Da-Sheng;Li, Yuan-Yi;Chen, Xiao-Jun;Li, Yu-Juan;Liu, Zhao-Ying;...

期刊： Journal of Pharmacological Sciences,2015年127(1):135-144 ISSN：1347-8613

通讯作者： Sun, Zhi-Liang

作者机构： [Li, Yu-Juan; Sun, Zhi-Liang; Zhang, Da-Sheng; Liu, Zhao-Ying; Li, Yuan-Yi; Chen, Xiao-Jun] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Xie, Wen-Jian] Hunan Univ Tradit Chinese Med, Dept Pharmaceut, Changsha 410128, Hunan, Peoples R China.;[Sun, Zhi-Liang] Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Sun, Zhi-Liang] H;Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.

关键词： Sanguinarine;ROS;Apoptosis;Methylation;MiR-15a/16-1

摘要： Previous studies show that several pathways are involved in sanguinarine-induced apoptotic cell death, including Ala downregulation, inhibition of NF-kB activation, mediation of ROS production, downregulation of anti-apoptosis proteins XIAP and clAP-1, upregulation of BAX, and downregulation of BCL2. In this study, we found out that the quenching of ROS generation by N-acetyl-L-cysteine (NAC), a scavenger of ROS, reversed sanguinarine-induced apoptosis effects, also we found out that sanguinarine-induced rat hepatic stellate T6 cells (HSC-T6 cells) apoptosis was correlated with the generation of increased ROS, which was followed by the activation of caspase-8 (-3, -6, and -9), and the decreasing in the miltochondrial membrane potential (MMP) and the down-regulation of anti-apoptotic protein Bcl-2. It is not clear whether BCL2's downregulation relates to its promoter methylation and miR-15a/16-1 expression which can bind to BCL2 3'-UTR (un-translation reagon). We showed that sanguinarine-induced down regulation of BCL2 was associated with the increased methylation rate of BCL2 promotor district and the increased expression of miR-15a/16-1. HSC-T6 cells treatment with 5-Aza-2'-deoxycytidine (5'-Aza-CdR) impeded sanguinarine-induced BCl2 promotor district methylation and recovered BCL2's expression. Over expression of BCL2 using pEGFP-N1 vector decreased sanguinarine-induced HSC-T6 cells apoptotic death significantly but not completely. These observations clearly showed that BCl2 down regulation was associated with its promoter methylation and miR-15a/16-1 upregulation in sanguinarine-induced Rat HSC-T6 cells. (C) 2014 Japanese Pharmacological Society. Production and hosting by Elsevier B.V.

语种： 英文

作者： 袁志航;刘兆颖;孙志良

作者机构： [袁志航; 刘兆颖; 孙志良] 湖南农业大学动物医学院湖南省兽药工程技术研究中心;[袁志航; 刘兆颖; 孙志良] 湖南省植物功能协同创新中心

会议名称： 中国畜牧兽医学会兽医药理毒理学分会第十一届会员代表大会暨第十三次学术讨论会与中国毒理学会兽医毒理专业委员会第五次学术研讨会

会议时间： 2015-10-20

会议地点： 中国湖南长沙

关键词： 钩吻素子;氧化应激凋亡

摘要： 为了探索钩吻生物碱单体钩吻素子对LPS诱导猪肠道上皮细胞损伤的修复作用。选用猪肠道上皮细胞(ZYM-SIEC-02细胞),筛选LPS致SIEC-02细胞损伤最佳剂量,建立损伤模型,作用不同浓度钩吻素子(0.05、0.5、5μg/ml)进行干预24h,检测细胞的增殖活性,以及细胞中TSOD、GSH、CAT活性、MDA水平和ROS含量的变化,并利用流式细胞仪检测细胞凋亡率。结果表明,与LPS模型组相比

语种： 中文

作者： 刘兆颖;伍勇;张大生;孙志良

作者机构： [刘兆颖; 伍勇; 张大生; 孙志良] 湖南省植物功能协同创新中心;[刘兆颖; 伍勇; 张大生; 孙志良] 湖南农业大学动物医学院湖南省兽药工程技术研究中心

会议名称： 中国畜牧兽医学会兽医药理毒理学分会第十一届会员代表大会暨第十三次学术讨论会与中国毒理学会兽医毒理专业委员会第五次学术研讨会

会议时间： 2015-10-20

会议地点： 中国湖南长沙

摘要： <正>血根碱是一种主要存在于罂粟科、蓝堇科及芸香科植物中的苯菲啶异喹啉类生物碱,具有抗菌、促进动物生长、抗肿瘤、抗炎及杀虫等作用。目前已有将其作为有效部位的中兽药上市,但血根碱本身具有一定的毒性,而其在机体内能被还原代谢成毒性较低的二氢血根碱,其中所参与的代谢酶和还原代谢机制尚未有研究报道,为此,研究团队鉴定了血根碱的代谢产物,并对其还原代谢机制进行了深入系统的机制研究,确定了醌氧化还原酶参与催化

语种： 中文

作者： 李玉娟;代佳娣;张大生;孙志良;刘兆颖

作者机构： [李玉娟; 代佳娣; 张大生; 孙志良; 刘兆颖] 湖南农业大学动物医学院湖南省兽药工程技术研究中心

会议名称： 中国畜牧兽医学会兽医药理毒理学分会第十一届会员代表大会暨第十三次学术讨论会与中国毒理学会兽医毒理专业委员会第五次学术研讨会

会议时间： 2015-10-20

会议地点： 中国湖南长沙

关键词： 氟喹诺酮类药物;达氟沙星;氧化应激;肾毒性;细胞周期

摘要： 有研究表明400μmol/L浓度范围内的达氟沙星处理LLC-PK1细胞后可降低细胞活力,同时过氧化氢酶（CAT）、超氧化物歧化酶（SOD）活性升高,在24h内并不引起LLC-PK1细胞的凋亡,但对其机制探讨鲜有报道。本试验研究了达氟沙星处理LLC-PK1细胞后对细胞的增殖抑制作用,O-2、H2O2、线粒体膜电位的改变,细胞周期以及相关基因表达含量的改变,探讨达氟沙星诱导LLC-PK1细胞氧

语种： 中文

作者： 谷宇锋;黄亚军;王琴;伍勇;刘兆颖;...

期刊： 中国畜牧兽医,2014年41(3):255-258 ISSN：1671-7236

作者机构： 湖南农业大学动物医学院;[孙志良] 湖南省兽药工程技术研究中心;湖南农业大学动物保健工程技术研究中心;[蒋政云; 谷宇锋; 王琴; 刘兆颖; 黄亚军; 张明军; 伍勇] 湖南农业大学

关键词： 复方淫羊藿;淫羊藿苷;正交设计;提取工艺

摘要： 为了优选复方淫羊藿的最佳提取工艺条件,本试验采用正交试验法,以药材加水量、提取次数和提取时间为自变量,以君药淫羊藿的指标成分淫羊藿苷的提取率为评价指标,进行数理统计选取最佳提取工艺。结果显示最佳提取工艺为加10倍药材质量的水,提取3次,每次提取1.5h。结果表明,该工艺为提高淫羊藿苷的提取率提供了试验基础,并为进一步的工业化生产提供科学理论依据。

语种： 中文

作者： Li, Juan;Huang, Lingli;Wang, Xu;Pan, Yuanhu;Liu, Zhaoying;...

期刊： Food and Chemical Toxicology,2014年69:109-119 ISSN：0278-6915

通讯作者： Yuan, Zonghui

作者机构： [Wang, Xu; Huang, Lingli; Tao, Yanfei; Wu, Qinghua; Yuan, Zonghui; Li, Juan; Pan, Yuanhu; Chen, Dongmei] Huazhong Agr Univ, Natl Reference Lab Vet Drug Residues HZAU, MAO Key Lab Detect Vet Drug Residues Foods, Wuhan 430070, Hubei, Peoples R China.;[Liu, Zhaoying] Hunan Agr Univ, Fac Vet, Changsha 410128, Hunan, Peoples R China.;[Wu, Qinghua] Univ Hradec Kralove, Fac Informat & Management, Ctr Basic & Appl Res, Hradec Kralove, Czech Republic.

通讯机构： [Yuan, Zonghui] H;Huazhong Agr Univ, Natl Reference Lab Vet Drug Residues HZAU, MAO Key Lab Detect Vet Drug Residues Foods, Wuhan 430070, Hubei, Peoples R China.

关键词： Disposition;Excretion;Metabolism;Quinocetone;Radioactivity isotope tracing;Toxicology

摘要： Excretion, disposition, and metabolism of [3H]-quinocetone in rats, pigs, broilers, and carp following oral administration were investigated. After a single p.o. dose, total radioactivity was rapidly excreted, with ≥94% in all species within 14days. Fecal excretion of radioactivity was 68% and 65% of the administered dose in rats and pigs, respectively, with the remainder excreted in the urine. Six hours after the last of seven daily oral administrations of 3H-labeled QCT, radioactivity was found to be distributed throughout all tissues, with the majority of radioactivity cleared within 7days, and elimination was the slowest from the liver and kidney. QCT was extensively metabolized in all of the species, and the primary changes included N-O group reduction, carbonyl group reduction, double bond reduction, and hydroxylation. The major tissue metabolites of QCT were Q2, Q4, Q5, Q8, and Q9 in rats; Q1, Q2, Q3, Q4, and Q5 in pigs; Q1, Q2, Q3, Q4, and Q7 in broilers; and Q1, Q2 in carp. This confirmed the potential link between QCT metabolism through N-O group reduction and its organ toxicity. The results of the present study provide important data that could help understand the relationship between the toxicities and metabolic disposition of QCT. © 2014 Elsevier Ltd.

语种： 英文