An analytical strategy to explore the multicomponent pharmacokinetics of herbal medicine independently of standards: Application in Gelsemium elegans extracts
作者：
Yang, Kun;Long, Xue-Ming;Cao, Jun-Jie;Li, Yu-Juan;Wu, Yong;...
期刊：
Journal of Pharmaceutical and Biomedical Analysis ,2019年176:112833 ISSN：0731-7085
通讯作者：
Sun, Zhi-Liang;Liu, Zhao-Ying
作者机构：
[Wu, Yong; Bai, Xia; Li, Yu-Juan; Sun, Zhi-Liang; Yang, Kun; Liu, Zhao-Ying; Cao, Jun-Jie] Hunan Agr Univ, Hunan Engn Technol Res Ctr Vet Drugs, Changsha 410128, Hunan, Peoples R China.;[Wu, Yong; Bai, Xia; Li, Yu-Juan; Sun, Zhi-Liang; Yang, Kun; Liu, Zhao-Ying; Cao, Jun-Jie] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Long, Xue-Ming] Hunan Prov Inst Vet Drugs & Feed Control, Changsha 410006, Hunan, Peoples R China.;[Sun, ZL; Liu, Zhao-Ying] Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Sun, ZL; Liu, ZY] H;Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.
关键词：
Alkaloids;Gelsemium elegans;Herbal medicine;LC-MS/MS;Pharmacokinetics
摘要：
The multicomponent pharmacokinetic study of herbal medicine is a great challenge due to the low plasma concentrations, large range of concentration scales, lack of authentic standards and uncertain interactions of the components. The aim of this work was to explore the in vivo pharmacokinetics of herbal medicine independently of authentic standards using an integrated analytical strategy. First, ion pairs of multiple components were tuned and selected, and then major parameters were optimized for derivative multiple reaction monitoring (DeMRM) by LC-MS/MS, which was combined with characterization of the chemical profiles of the herbal medicine by LC-QqTOF/MS. Second, different concentrations of herbal extracts were employed instead of authentic standards to construct calibration curves for the semiquantitative determination of multiple components in plasma. Taking Gelsemium elegans as an example, in addition to the fully validated and sufficient methodological results, a total of 27 alkaloid components, major bioactive constituents of Gelsemium elegans, were simultaneously monitored in pig plasma. The concentration-time profiles and pharmacokinetic properties of these 27 components were characterized. The absolute quantification of three components was compared with the results obtained using authentic standards, and the method showed very similar analytical characteristics, such as linearity, precision, accuracy, and the values of the pharmacokinetic parameters Tmax, Vd, Cl and MRT. This analytical strategy was found to be capable of assessing herbal pharmacokinetics independently of specific authentic compounds for each component. This study was the first attempt to systematically reveal the in vivo pharmacokinetics of Gelsemium elegans. This strategy and methodology will find widespread use in the quantitative pharmacokinetic analysis of multiple components independently of standards for herbal medicine, among other applications.
语种：
英文
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钩吻素子在猪的体外代谢
作者：
肖洒;黄亚军;刘艳纯;孙志良;刘兆颖
期刊：
中国兽医学报 ,2018年38(8):1568-1572 ISSN：1005-4545
作者机构：
[肖洒; 黄亚军; 刘艳纯; 孙志良; 刘兆颖] 湖南农业大学动物医学院, 湖南省兽药工程技术研究中心, 湖南, 长沙, 410128
关键词：
钩吻素子;猪;体外代谢
摘要：
研究钩吻素子在猪体外肝S9的代谢,采用高效液相色谱串联四极杆飞行时间质谱(HPLC/QqTOF MS)对孵育后的代谢物进行结构鉴定。结果表明,钩吻素子在猪肝S9中可生成7种代谢产物,根据原形及其代谢物的精确分子质量差、产物离子,鉴定了代谢物结构,包括N-脱甲基化代谢物(M1)、C18和C19的加氢代谢物(M3)、M3的去甲基代谢物(M4)、氧化代谢物(M6、M8和M12)和脱氢代谢物(M13)。钩吻素子在猪的代谢途径主要是氧化、还原、 N-脱甲基化。其研究结果为钩吻素子在动物及人体内代谢研究奠定基础。
语种：
中文
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Identification of gelsemine metabolites in rat liver S9 by high-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry
作者：
Yang, Kun;Huang, Ya-Jun;Xiao, Sa;Liu, Yan-Chun;Sun, Zhi-Liang;...
期刊：
Rapid Communications in Mass Spectrometry ,2018年32(1):19-22 ISSN：0951-4198
通讯作者：
Liu, Zhao-Ying
作者机构：
[Sun, Zhi-Liang; Yang, Kun; Liu, Zhao-Ying; Xiao, Sa; Huang, Ya-Jun] Hunan Agr Univ, Coll Vet Med, Hunan Engn Res Ctr Vet Drug, Changsha 410128, Hunan, Peoples R China.;[Tang, Qi; Liu, Zhao-Ying; Liu, Yi-Song; Liu, Yan-Chun] Hunan Agr Univ, Natl & Local Union Engn Res Ctr Vet Herbal Med Re, Changsha 410128, Hunan, Peoples R China.;[Sun, Zhi-Liang; Liu, Zhao-Ying] Hunan Agr Univ, Coll Vet Med, Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhao-Ying] Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liu, Zhao-Ying] H;Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.
摘要：
Rationale: Gelsemine has been extensively studied because of its anti-tumor, immunomodulatory, insecticidal itching and other significant effects. However, limited information on the pharmacokinetics and metabolism of gelsemine has been reported. Therefore, the purpose of the present study was to investigate the in vitro metabolism of gelsemine in rat liver S9 by using rapid and accurate high-performance liquid chromatography/ quadrupole-time-of-flight mass spectrometry (HPLC/QqTOF-MS). Methods: The incubation mixture was processed with 15% trichloroacetic acid. Multiple scans of gelsemine metabolites and accurate mass measurements were automatically performed simultaneously through data-dependent acquisition in only 30 min. The structural elucidations of these metabolites were performed by comparing their changes in accurate molecular masses and product ions with those of the parent drug. Results: Five metabolites of gelsemine were identified in rat liver S9. Of these, four metabolites of gelsemine were identified for the first time. The present results showed that the metabolic pathways of gelsemine are oxidation, demethylation, and dehydrogenation in rat liver S9. Conclusions: In this study, metabolites of gelsemine in liver S9 were identified and elucidated firstly using the HPLC/QqTOF-MS method. The proposed metabolic pathways of gelsemine in liver S9 will provide a basis for further studies of the in vivo metabolism of gelsemine in animals and humans. Copyright © 2017 John Wiley & Sons, Ltd.
语种：
英文
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Biotransformation and tissue distribution of protopine and allocryptopine and effects of Plume Poppy Total Alkaloid on liver drug-metabolizing enzymes
作者：
Huang, Ya-Jun;Cheng, Pi;Zhang, Zhuo-Yi;Tian, Shi-Jie;Sun, Zhi-Liang;...
期刊：
Scientific Reports ,2018年8(1):537 ISSN：2045-2322
通讯作者：
Liu, Zhao-Ying
作者机构：
[Tian, Shi-Jie; Sun, Zhi-Liang; Zeng, Jian-Guo; Liu, Zhao-Ying; Cheng, Pi; Huang, Ya-Jun] Hunan Agr Univ, Vet Herbal Med Resources & Initiat, Natl & Local Union Engn Res Ctr, Changsha 410128, Hunan, Peoples R China.;[Sun, Zhi-Liang; Liu, Zhao-Ying; Zhang, Zhuo-Yi; Huang, Ya-Jun] Hunan Agr Univ, Coll Vet Med, Hunan Engn Res Ctr ofVeterinary Drug, Changsha 410128, Hunan, Peoples R China.;[Zeng, Jian-Guo; Liu, Zhao-Ying] Hunan Agr Univ, Hunan Key Lab Tradit Chinese Vet Med, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liu, Zhao-Ying] H;Hunan Agr Univ, Vet Herbal Med Resources & Initiat, Natl & Local Union Engn Res Ctr, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Coll Vet Med, Hunan Engn Res Ctr ofVeterinary Drug, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Hunan Key Lab Tradit Chinese Vet Med, Changsha 410128, Hunan, Peoples R China.
摘要：
In this study, the biotransformation in the plasma, urine and feces of rats following oral administration of protopine (PRO) and allocryptopine (ALL)were explored using HPLC-QqTOF MS. An HPLC-MS/MS method for the determination of tissues was developed and applied to the tissue distribution study in rats following intragastric administration of Plume Poppy Total Alkaloid for 3 weeks. A total of ten PRO metabolites and ten ALL metabolites were characterized in rats in vivo. Among these metabolites, six PRO metabolites and five ALL metabolites were reported for the first time. The predicated metabolic pathways including ring cleavage, demethylation following ring cleavage, and glucuronidation were proposed. The low-concentration residue of PRO and ALL in various tissues was detected at 24 h and 48 h after dosing, which indicated that both compounds could be widely distributed in tissues and exist as low levels of residue. The activities of erythromycin N-demethylase, aminopyrine N-demethylase and NAD (P)H quinone oxidoreductase in female rats can be induced post-dose, but these activities were inhibited in male rats. The proposed biotransformation and residues of PRO and ALL and their effects on enzymes may provide a basis for clarifying the metabolism and interpreting pharmacokinetics.
语种：
英文
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不同产地博落回果化学成分指纹图谱研究
作者：
胡南希;郑亚杰;唐其;刘兆颖;刘薇;...
期刊：
中草药 ,2018年49(18):4417-4423 ISSN：0253-2670
通讯作者：
Zheng, Y.-J.
作者机构：
湖南农业大学兽用中药资源与中兽药创制国家地方联合工程研究中心,湖南长沙410128;湖南农业大学动物医学院,湖南长沙410128;湖南农业大学园艺园林学院,湖南长沙410128;湖南农业大学兽用中药资源与中兽药创制国家地方联合工程研究中心,湖南长沙,410128;[刘薇] 刘薇
通讯机构：
[Zheng, Y.-J.] N;National and Local Union Engineering Research Center for the Veterinary Herbal Medicine Resources and Initiative, Hunan Agricultural University, Changsha, China
关键词：
博落回果;指纹图谱;产地;主成分分析;聚类分析;原阿片碱;别隐品碱;血根碱;白屈菜红碱
摘要：
目的构建博落回Macleaya cordata果化学成分指纹图谱,建立博落回果品质评价方法。方法根据湖南省长沙市地方标准2009年版测定博落回中原阿片碱、别隐品碱、血根碱和白屈菜红碱含量;采用高效液相色谱法构建基于不同产地的博落回果化学成分指纹图谱;利用主成分分析和聚类分析方法,分析博落回果中共有指纹峰、产地和药材品质的关联性。结果构建不同产地博落回果化学成分指纹图谱,确定11个共有指纹峰;主成分分析和聚类分析将8个产地的博落回果分为2大类,其中共有峰5、7、8、9和11对指纹图谱差异贡献较大,6号峰是指纹图谱参照峰的最佳选择。结论本实验建立的博落回果化学成分指纹图谱具有良好的精密度、重复性和稳定性,能够对不同产地博落回果进行品质评价。
语种：
中文
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Characterization of in vitro metabolites of three tetrahydroprotoberberine alkaloids in rat liver S9 by high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry
作者：
Lin, Li;Liu, Xiu-Bin;Zhou, Qiao-Yun;Liu, Sha-Sha;Zuo, Meng-Ting;...
期刊：
Rapid Communications in Mass Spectrometry ,2018年32(17):1540-1548 ISSN：0951-4198
通讯作者：
Liu, Zhao-Ying
作者机构：
[Zeng, Jian-Guo; Liu, Zhao-Ying; Liu, Xiu-Bin; Lin, Li] Hunan Agr Univ, Hunan Key Lab Tradit Chinese Vet Med, Changsha 410128, Hunan, Peoples R China.;[Zhou, Qiao-Yun; Liu, Sha-Sha; Liu, Zhao-Ying; Lin, Li; Zuo, Meng-Ting] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhao-Ying; Zuo, Meng-Ting] Hunan Agr Univ, Hunan Engn Res Ctr Vet Drug, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liu, Zhao-Ying] H;Hunan Agr Univ, Hunan Key Lab Tradit Chinese Vet Med, Changsha 410128, Hunan, Peoples R China.
摘要：
Rationale: Tetrahydroberberine (THB), tetrahydrocoptisine (THCP) and tetrahydrocolumbamine (THCB) belong to the tetrahydroprotoberberine (THPB) alkaloids. Most of them have been extensively studied because of their pharmacological activities such as anti-hypertension, anti-arrhythmia, antimicrobial activity and antioxidant. However, limited information on the pharmacokinetics and metabolism of the three alkaloids has been reported. The purpose of this study was to investigate the in vitro metabolism of THB, THCP and THCB in rat liver S9 by using a rapid and accurate high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (HPLC/QqTOF-MS) method. Methods: The incubation mixture was processed with 15% trichloroacetic acid. Chromatographic separation of the three THPB alkaloids and their metabolites was achieved by HPLC/QqTOF-MS and accurate mass measurements of metabolites were automatically performed through data-dependent acquisition in only a 30-min analysis. The detailed structural elucidations of these metabolites were performed by comparing the changes in their accurate molecular masses, elemental compositions and product ions with those of the parent drug. Results: Five, five and four metabolites of THB, THCP and THCB were identified in rat liver S9, respectively. The results show that O-demethylenation of the 9,10-vicinal methoxyl group was the main metabolic pathway of THB and THCB and that demethylenation of the two methylenedioxy groups was the main metabolic pathway of THCP. In addition, minor oxidation and methylation reactions could occur for these alkaloids in rat liver S9. Conclusions: This was the first investigation of the in vitro metabolism of THB, THCP and THCB in rat liver S9 by using a sensitive and accurate HPLC/QqTOF-MS method. The tentatively proposed metabolic pathways of the three alkaloids will provide a basis for further studies of the in vivo metabolism of the three compounds in animals and humans. © 2018 John Wiley & Sons, Ltd.
语种：
英文
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Characterization of N-methylcanadine and N-methylstylopine metabolites in rat liver S9 by high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry
作者：
Zuo, Meng-Ting;Liu, Sha-Sha;Lin, Li;Wang, Zi-Yuan;Bai, Xia;...
期刊：
Rapid Communications in Mass Spectrometry ,2018年32(23):2047-2054 ISSN：0951-4198
通讯作者：
Liu, Zhao-Ying
作者机构：
[Bai, Xia; Liu, Sha-Sha; Wang, Zi-Yuan; Sun, Zhi-Liang; Liu, Zhao-Ying; Zuo, Meng-Ting] Hunan Agr Univ, Hunan Engn Res Ctr Vet Drug, Changsha 410128, Hunan, Peoples R China.;[Bai, Xia; Liu, Sha-Sha; Sun, Zhi-Liang; Liu, Zhao-Ying; Lin, Li; Zuo, Meng-Ting] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhao-Ying] Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liu, Zhao-Ying] H;Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.
摘要：
Rationale: N-Methylcanadine and N-methylstylopine are two types of isoquinoline alkaloids which are considered to be the main medicinally active constituents of the genus Papaveraceae. However, to date, no metabolism studies of N-methylcanadine and N-methylstylopine have been reported. Therefore, the purpose of the present study was to investigate the in vitro metabolism of these two alkaloids in rat liver S9. Methods: N-Methylcanadine or N-methylstylopine was incubated with rat liver S9 for 1 h, and then the incubation mixture was processed with 15% trichloroacetic acid. High-performance liquid chromatography with quadrupole time-of-flight mass spectrometry (HPLC/QqTOF-MS) as a reliable analytical method was used. The structural characterization of these metabolites was performed by the combination of the accurate MS/MS spectra and the known elemental composition. Results: As a result, a total of four metabolites of N-methylcanadine and five metabolites of N-methylstylopine in rat liver S9 were tentatively identified. The cleavage of the methylenedioxy group of the drugs was the main metabolic pathway of N-methylcanadine and N-methylstylopine. Conclusions: The present study is the first in vitro metabolic investigation of N-methylcanadine and N-methylstylopine in rat liver S9 using a reliable HPLC/QqTOF-MS method. The metabolic pathways of N-methylcanadine and N-methylstylopine are tentatively proposed. This work lays the foundation for the in vivo metabolism of the two compounds in animals. © 2018 John Wiley & Sons, Ltd.
语种：
英文
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Development and in-house validation of a sensitive LC–MS/MS method for simultaneous quantification of gelsemine, koumine and humantenmine in porcine plasma
作者：
Yang, Kun;Long, Xue-Ming;Liu, Yan-Chun;Chen, Fu-Hua;Liu, Xiao-Feng;...
期刊：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES ,2018年1076:54-60 ISSN：1570-0232
通讯作者：
Sun, Zhi-Liang;Liu, Zhao-Ying
作者机构：
[Sun, Zhi-Liang; Yang, Kun; Liu, Zhao-Ying; Liu, Yan-Chun; Liu, Xiao-Feng] Hunan Agr Univ, Coll Vet Med, Hunan Engn Res Ctr Vet Drug, Changsha 410128, Hunan, Peoples R China.;[Chen, Fu-Hua; Long, Xue-Ming] Inst Hunan Prov Vet Drugs & Feed Supervis, Changsha 410006, Hunan, Peoples R China.;[Sun, Zhi-Liang; Liu, Zhao-Ying] Hunan Agr Univ, Coll Vet Med, Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhao-Ying] Hunan Agr Univ, Natl & Local Union Engn Res Ctr Vet Herbal Med Re, Changsha 410128, Hunan, Peoples R China.;[Sun, ZL; Liu, Zhao-Ying] Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Sun, ZL; Liu, ZY] H;Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.
关键词：
Gelsemine;Gelsemium elegans;Humantenmine;Indole alkaloid;Koumine;Plasma
摘要：
Three monomers of G. elegans indole alkaloids (gelsemine, koumine and humantenmine) were simultaneously detected in porcine plasma for the first time with the development and validation of a sensitive and reliable LC-ESI-MS/MS method. Using a gradient mobile phase at a constant flow rate of 0.2 mL/min via electrospray ionization (positive ion mode) in a multiple reaction monitoring (MRM) scan, gelsemine, koumine and humantenmine were eluted, separated and detected at an appropriate retention time. The porcine plasma was prepared using protein precipitation with 1% formic acid-acetonitrile: methanol (2:1, v/v). Using matrix-matched calibration curves and weighted least squares linear regression, a good linearity (r<sup>2</sup>>0.99) was achieved with a concentration range of 0.1–200 μg/L for gelsemine, koumine and humantenmine;estimated LOD and LOQ values were 0.10 μg/L and 0.2 μg/L, respectively. The mean of the recoveries was in the range of 82.68–100.35% of porcine plasma at four different levels, and the intra-day and inter-day precision (CV) were lower than 15% with a range of 2.46–8.76% and 2.73–10.83%, respectively. The proposed method has proved to be suitable for accurate, quantitative determination of gelsemine, koumine and humantenmine in porcine plasma.<br/> ©2018
语种：
英文
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现代教育技术在《动物生物化学》课程教学中的应用
作者：
柳亦松;苏建明;黄复深;刘兆颖;谭超
期刊：
高教学刊 ,2018年(17):69-71 ISSN：2096-000X
作者机构：
湖南农业大学 动物医学院,湖南 长沙,410128;[柳亦松; 刘兆颖; 黄复深; 苏建明; 谭超] 湖南农业大学
关键词：
动物生物化学;现代教育技术;教学改革;虚拟仿真
摘要：
动物生物化学是畜牧和水产相关专业的一门重要基础课程,如何让学生更好地掌握这门课程的内容,关系到学生后续专业课程的学习和理解,以及学校对相关专业人才的培养。文章在充分掌握动物生物化学课程的教学目标、特点和实际教学情况的基础上,以现代教育技术为手段,从改善教学条件、完善教学体系、加强实践培养、教学科研相结合等方面探讨对课程的改革,可以极大地提高了学生的兴趣,以及提出问题、分析问题、解决问题的能力和学习的主动性和积极性,同时可以全面提高课程的学习过程中学生对于课程的体验。
语种：
中文
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二氢血根碱和二氢白屈菜红碱在鸡肌肉中的定量检测方法
作者：
陈梅;张换成;柳亦松;刘兆颖;曾建国
期刊：
中国兽医学报 ,2018年38(9):1766-1770 ISSN：1005-4545
作者机构：
湖南农业大学动物医学院, 兽用中药资源与中兽药创制国家地方联合工程研究中心, 湖南, 长沙, 410128;湖南农业大学动物医学院, 湖南省兽药工程技术研究中心, 湖南, 长沙, 410128;[陈梅; 张换成; 柳亦松; 曾建国] 湖南农业大学动物医学院, 兽用中药资源与中兽药创制国家地方联合工程研究中心, 湖南, 长沙, 410128;[刘兆颖] 湖南农业大学动物医学院, 兽用中药资源与中兽药创制国家地方联合工程研究中心, 湖南, 长沙, 410128 湖南农业大学动物医学院, 湖南省兽药工程技术研究中心, 湖南, 长沙, 410128
关键词：
二氢血根碱;二氢白屈菜红碱;博落回散;残留分析
摘要：
建立了一种用于同时测定鸡肌肉中二氢血根碱和二氢白屈菜红碱的UPLC-MS/MS方法.肌肉样品经乙腈-水(95∶5)溶液超声提取后,离心取上清液经脱脂后氮吹浓缩,甲醇复溶.采用Agilent Poroshell 120 EC-C18(2.1 mm×150 mm i.d.,2.7 μm)色谱柱分离,采用正离子化模式下的多反应监测模式进行测定.方法的特异性良好,二氢血根碱和二氢白屈菜红碱在0.2～200 μg/L质量浓度范围内线性关系良好(r2≥0.999 6),最低定量限均为0.4 μg/kg,平均回收率分别为103.44％和104.36％,准确度RE均≤10.49％,精密度RSD均≤7.43％.该方法应用于长期饲喂博落回散的健康雌性白羽肉鸡的鸡胸肌组织中二氢血根碱和二氢白屈菜红碱的残留量检测,在500 mg/kg博落回散组测得DHSA残留量为0.804 μg/kg.该方法灵敏度高,重复性好,适用于鸡肌肉中二氢血根碱和二氢白屈菜红碱的残留定量检测.
语种：
中文
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Medicinal plants of the genus Macleaya (Macleaya cordata, Macleaya microcarpa): A review of their phytochemistry, pharmacology, and toxicology
作者：
Lin, Li;Liu, Yan-Chun;Huang, Jia-Lu;Liu, Xiu-Bin;Qing, Zhi-Xing;...
期刊：
Phytotherapy Research ,2018年32(1):19-48 ISSN：0951-418X
通讯作者：
Zeng, Jian-Guo;Liu, Zhao-Ying
作者机构：
[Zeng, Jian-Guo; Liu, Zhao-Ying; Huang, Jia-Lu; Lin, Li; Liu, Yan-Chun] Hunan Agr Univ, Natl & Local Union Engn Res Ctr Vet Herbal Med Re, Changsha 410128, Hunan, Peoples R China.;[Qing, Zhi-Xing; Zeng, Jian-Guo; Liu, Zhao-Ying; Liu, Xiu-Bin] Hunan Agr Univ, Coll Vet Med, Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhao-Ying] Hunan Agr Univ, Coll Vet Med, Hunan Engn Res Ctr Vet Drug, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Zeng, JG; Liu, ZY; Liu, Zhao-Ying] H;Hunan Agr Univ, Coll Vet Med, Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Coll Vet Med, Hunan Engn Res Ctr Vet Drug, Changsha 410128, Hunan, Peoples R China.
关键词：
alkaloids;Macleaya;pharmacology;phytochemistry;toxicology
摘要：
In the genus Macleaya, Macleaya cordata and Macleaya microcarpa have been recognized as traditional herbs that are primarily distributed in China, North America, and Europe and have a long history of medicinal usage. These herbs have been long valued and studied for detumescence, detoxification, and insecticidal effect. This review aims to provide comprehensive information on botanical, phytochemical, pharmacological, and toxicological studies on plants in the genus Macleaya. Plants from the genus of Macleaya provide a source of bioactive compounds, primarily alkaloids, with remarkable diversity and complex architectures, thereby having attracted attention from researchers. To date, 291 constituents have been identified and/or isolated from this group. These purified compounds and/or crude extract possess antitumor, anti-inflammatory, insecticidal, and antibacterial activities in addition to certain potential toxicities. Macleaya species hold potential for medicinal applications. However, despite the pharmacological studies on these plants, the mechanisms underlying the biological activities of active ingredients derived from Macleaya have not been thoroughly elucidated to date. Additionally, there is a need for research focusing on in vivo medical effects of Macleaya compounds and, eventually, for clinical trials.
语种：
英文
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Structural elucidation of koumine metabolites by accurate mass measurements using high-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry
作者：
Xiao, Sa;Huang, Ya-Jun;Sun, Zhi-Liang;Liu, Zhao-Ying*
期刊：
Rapid Communications in Mass Spectrometry ,2017年31(3):309-314 ISSN：0951-4198
通讯作者：
Liu, Zhao-Ying
作者机构：
[Sun, Zhi-Liang; Liu, Zhao-Ying; Xiao, Sa; Huang, Ya-Jun] Hunan Agr Univ, Coll Vet Med, Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha 410128, Hunan, Peoples R China.;[Sun, Zhi-Liang; Xiao, Sa; Huang, Ya-Jun] Hunan Agr Univ, Coll Vet Med, Hunan Engn Res Ctr Vet Drug, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhao-Ying] Hunan Agr Univ, Natl & Local Union Engn Res Ctr Vet Herbal Med Re, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhao-Ying] Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liu, Zhao-Ying] H;Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.
摘要：
Rationale: Koumine is one of the major components of total alkaloids from Gelsemium. Koumine possesses a variety of interesting pharmacological effects, including anti-tumor, anti-inflammatory, and anxiolytic activities. It might be a promising lead drug because of its pharmacological activities and mild toxicity. However, little information is available on the metabolism of koumine. Methods: A rapid and accurate high-performance liquid chromatography/quadrupole-time-of-flight (HPLC/QqTOF) mass spectrometry method was applied to characterize koumine metabolites. Multiple scans of koumine metabolites, which were formed in rat liver S9, were automatically performed simultaneously through auto MS/MS mode acquisition in only a 30-min analysis. The structural elucidation of these metabolites was performed by comparing their changes in accurate molecular masses and product ions with those of the parent drug or metabolites. Results: As a result, a total of eleven metabolites of koumine were identified, of which nine new metabolites were found. The present results showed that the N-demethylenation, hydrogenation and the oxidation were the three main metabolic pathways of koumine. Conclusions: This was the first investigation of in vitro metabolism of koumine in rat liver S9 using a sensitive and specific HPLC/QqTOF-MS method. The possible metabolic pathways of koumine were tentatively proposed based on the structural elucidations of these metabolites. This work may be useful in the in vivo metabolism of koumine in animals and humans. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.
语种：
英文
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血根碱和白屈菜红碱药代动力学研究进展
作者：
田世杰;刘兆颖;柳亦松;曾建国
期刊：
红领巾(探索) ,2017年(1):57-60 ISSN：1005-3514
作者机构：
湖南农业大学动物医学院;[曾建国] 兽用中药资源与中兽药创制国家地方联合工程研究中心;植物功能成分利用协同创新中心;[田世杰; 柳亦松; 刘兆颖] 湖南农业大学
关键词：
血根碱(SA);白屈菜红碱(CHE);博落回;脂质体;药代动力学
摘要：
血根碱（SA）和白屈菜红碱（CHE）为苯并菲啶类生物碱,主要存在于博落回等罂粟科植物中,具有抗肿瘤、抗炎、促进生长等多种生物学活性。人们对它们在动物体内吸收、分布、代谢、排泄及体外代谢进行了深入研究。大量研究表明,SA和CHE在动物体内和体外主要通过氧化、还原或结合葡萄糖醛酸等方式转化为代谢物。体内消除时间短,以原形或代谢物形式排出体外。文章对近10年来SA和CHE的药物代谢动力学研究进行了综述。
语种：
中文
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钩吻素子对LPS诱导RAW264.7细胞炎症的作用
作者：
袁志航;袁慧;邬静;陈小军;刘兆颖;...
期刊：
中国兽医学报 ,2017年37(8):1553-1557 ISSN：1005-4545
作者机构：
湖南农业大学动物医学院, 湖南兽药工程技术研究中心, 湖南, 长沙, 410128;湖南农业大学动物医学院, 湖南, 长沙, 410128;[袁志航; 邬静; 陈小军; 刘兆颖; 孙志良] 湖南农业大学动物医学院, 湖南兽药工程技术研究中心, 湖南, 长沙, 410128;[袁慧] 湖南农业大学动物医学院, 湖南, 长沙, 410128
关键词：
钩吻素子;脂多糖;炎症;巨噬细胞
摘要：
为揭示钩吻素子的体外抗炎作用机理,利用硝酸还原酶法和ELISA法研究了不同质量浓度(100,200,400mg/L)的钩吻素子对一氧化氮(NO)和细胞因子白介素-1β(IL-1β)、白介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)分泌的影响,运用了QPCR的方法检测了钩吻素子对诱导型一氧化氮合成酶(iNOS)、IL-1β、IL-6和TNF-αmRNA表达水平的影响,并运用Western blot法检测了钩吻素子对iNOS蛋白表达的影响。结果显示,100,200,400mg/L质量浓度的钩吻素子能够极显著的抑制RAW264.7细胞NO的产生以及IL-1β、IL-6和TNF-α的分泌(P<0.01),同时能够剂量依赖性的降低iNOS、IL-1β、IL-6和TNF-α的mRNA水平,抑制脂多糖(LPS)引起的iNOS蛋白表达的升高(P < 0.01)。由此推论,钩吻素子可能通过抑制炎症因子的分泌,减少NO的释放,下调iNOS、IL-1β、IL-6和TNF-α的mRNA水平,抑制iNOS蛋白过度表达达到其抗炎作用。
语种：
中文
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血根碱对大鼠结肠炎及炎症通路中相关因子的影响
作者：
柳亦松;唐昭山;刘兆颖;蒋昊航;段志贵;...
期刊：
中国现代中药 ,2017年19(10):1391-1396 ISSN：1673-4890
作者机构：
湖南农业大学动物医学院,湖南 长沙410128;湖南农业大学兽用中药资源与中兽药创制国家地方联合工程研究中心,湖南长沙410128;湖南农业大学动物医学院,湖南 长沙,410128;湖南师范大学生命科学学院,湖南 长沙,410081;湖南省植物功能成分利用协同创新中心,湖南长沙410128
关键词：
血根碱;结肠炎;炎症因子
摘要：
目的:探讨血根碱对葡聚糖硫酸钠致溃疡性结肠炎的疗效及可能的机制。方法:建立葡聚糖硫酸钠致溃疡性结肠炎大鼠模型,随机分为空白组、模型组、柳氮磺吡啶组、血根碱组。观察各组大鼠体质量、腹泻及隐血情况;通过荧光定量PCR（qPCR）分析炎症因子IL-1β、IL-6、IL-10、TNF-α在结肠组织中的表达量;利用免疫组化实验检测NF-κB通路中关键因子p65。结果:与模型组相比,血根碱组和阳性组大鼠的结肠组织中炎症因子IL-1β、IL-6表达降低,而IL-10的表达升高;同时,免疫组化实验发现血根碱组与模型组相比,其核内转入因子核蛋白p65显著下调。结论:血根碱可能通过调节NF-κB通路中的核转入因子p65,降低炎症因子IL-1β、IL-6的表达,同时提高炎症因子IL-10的表达,从而对炎症起到抑制作用。
语种：
中文
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钩吻的历史溯源及植物学特征
作者：
张卓亿;代佳娣;杨昆;孙志良;刘兆颖
期刊：
中兽医医药杂志 ,2017年36(6):78-82 ISSN：1000-6354
作者机构：
湖南省植物功能协同创新中心湖南农业大学动物医学院,湖南长沙,410128;[代佳娣; 刘兆颖; 张卓亿; 杨昆; 孙志良] 湖南农业大学
关键词：
钩吻;植物学特征;传统应用
摘要：
钩吻具有广泛的药理活性,在中兽药领域的应用前景广阔。自古以来,国内外对钩吻的记载纷繁多样,说法不一。本文就钩吻的历史溯源、植物学特征及传统应用方面进行梳理,为钩吻的安全使用及开发提供理论依据。
语种：
中文
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在模拟人工胃肠道环境中博落回主要生物碱类成分的稳定性特征分析
作者：
张换成;陈梅;柳亦松;唐昭山;曾建国;...
期刊：
中国现代中药 ,2017年19(10):1387-1390,1414 ISSN：1673-4890
作者机构：
湖南农业大学兽用中药资源与中兽药创制国家地方联合工程研究中心;湖南农业大学动物医学院、湖南省兽药工程技术研究中心;[唐昭山] 湖南美可达生物资源股份有限公司;[曾建国; 柳亦松; 张换成; 刘兆颖; 陈梅] 湖南农业大学
关键词：
博落回;血根碱;白屈菜红碱;胃肠道稳定性
摘要：
目的：为解释博落回中4个生物碱（原阿片碱、别隐品碱、血根碱、白屈菜红碱）生物利用低的原因,对这四个生物碱在模拟人工胃肠道环境下的稳定性进行考察。方法：采用HPLC测定博落回中4个生物碱在不同p H缓冲液、人工胃液（p H=1.3）和人工肠液（p H=6.8）中的稳定性。结果：原阿片碱和别隐品碱在酸性、中性、碱性、人工胃液及人工肠液环境下8 h回收率为97.16%~101.89%;血根碱在p H=6.8磷酸盐缓冲液、水、p H=7.8~8.0磷酸盐缓冲液及人工肠液中8 h回收率为48.81%~60.53%;白屈菜红碱在p H=5.8磷酸盐缓冲液、p H=6.8磷酸盐缓冲液、水及人工肠液中8 h的回收率为81.21%~93.48%;在p H=7.8~8.0磷酸盐缓冲液中8 h回收率为57.43%。结论：原阿片碱和别隐品碱的稳定性不受p H的影响,血根碱和白屈菜红碱随p H升高稳定性降低,博落回中4个生物碱在模拟人工胃肠道环境中的稳定性结果与其在不同p H环境中一致,为阐明博落回散在体内的药代动力学特点提供科学依据。
语种：
中文
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Comprehensive identification and structural characterization of target components from Gelsemium elegans by high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry based on accurate mass databases combined with MS/MS spectra
作者：
Liu, Yan-Chun;Xiao, Sa;Yang, Kun;Ling, Li;Sun, Zhi-Liang;...
期刊：
JOURNAL OF MASS SPECTROMETRY ,2017年52(6):378-396 ISSN：1076-5174
通讯作者：
Liu, Zhao-Ying
作者机构：
[Sun, Zhi-Liang; Yang, Kun; Liu, Zhao-Ying; Liu, Yan-Chun; Ling, Li; Xiao, Sa] Hunan Agr Univ, Hunan Engn Res Ctr Vet Drug, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Sun, Zhi-Liang; Liu, Zhao-Ying] Hunan Agr Univ, Coll Vet Med, Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhao-Ying] Hunan Agr Univ, Natl & Local Union Engn Res Ctr Vet Herbal Med Re, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhao-Ying] Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liu, Zhao-Ying] H;Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.
关键词：
accurate mass databases;Gelsemium;quadrupole time-of-flight mass spectrometry;traditional Chinese medicine;alkaloid
摘要：
This study reports an applicable analytical strategy of comprehensive identification and structure characterization of target components from Gelsemium elegans by using high-performance liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QqTOF MS) based on the use of accurate mass databases combined with MS/MS spectra. The databases created included accurate masses and elemental compositions of 204 components from Gelsemium and their structural data. The accurate MS and MS/MS spectra were acquired through data-dependent auto MS/MS mode followed by an extraction of the potential compounds from the LC-QqTOF MS raw data of the sample. The same was matched using the databases to search for targeted components in the sample. The structures for detected components were tentatively characterized by manually interpreting the accurate MS/MS spectra for the first time. A total of 57 components have been successfully detected and structurally characterized from the crude extracts of G. elegans, but has failed to differentiate some isomers. This analytical strategy is generic and efficient, avoids isolation and purification procedures, enables a comprehensive structure characterization of target components of Gelsemium and would be widely applicable for complicated mixtures that are derived from Gelsemium preparations. Copyright ©2017 John Wiley &Sons, Ltd. Copyright ©2017 John Wiley & Sons, Ltd.
语种：
英文
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四种猪可食性组织中巴氯芬定量/确证方法研究
作者：
杨昆;周宇俊;易远胜;刘艳纯;孙志良;...
作者机构：
[杨昆; 周宇俊; 易远胜; 刘艳纯; 孙志良; 刘兆颖] 湖南农业大学动物医学院
会议名称：
中国毒理学会兽医毒理学委员会与中国畜牧兽医学会兽医食品卫生学分会联合学术研讨会暨中国毒理学会兽医毒理学委员会第5次全国会员代表大会
会议时间：
2017-09-22
会议地点：
中国北京
会议论文集名称：
中国毒理学会兽医毒理学委员会与中国畜牧兽医学会兽医食品卫生学分会联合学术研讨会暨中国毒理学会兽医毒理学委员会第5次全国会员代表大会会议论文集
关键词：
巴氯芬;定量;可食性组织;液质联用
摘要：
【目的】本试验采用三重四级杆串联质谱,对具有镇静、催眠、促生长作用而未获得批准应用于兽药临床的巴氯芬进行定量/确证方法研究,以期能应用于猪可食性组织中对巴氯芬残留监控。【方法】采用酸性乙酸铵将巴氯芬水解游离后,沉淀可溶性蛋白后,用异丙醇-乙酸乙酯将游离型巴氯芬液液萃取,进一步用阳离子SPE固相萃取净化巴氯芬,以m/z 214.1>179.0*和m/z214·1>151·0为反应监测离子对,采用多反
语种：
中文
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Determination of Baclofen Residue in Muscle, Liver, Kidney and Fat of Swine by Liquid Chromatography-Tandem Mass Spectrometry
作者：
Yang, Kun;Zhou, Yu-Jun;Chen, Fu-Hua;Long, Xue-Ming;Kuang, Guang-Wei;...
期刊：
Food Analytical Methods ,2017年10(12):3866-3873 ISSN：1936-9751
通讯作者：
Sun, Zhi-Liang;Liu, Zhao-Ying
作者机构：
[Sun, Zhi-Liang; Zhou, Yu-Jun; Yang, Kun; Liu, Zhao-Ying; Sun, ZL] Hunan Agr Univ, Coll Vet Med, Hunan Engn Res Ctr Vet Drug, Changsha 410128, Hunan, Peoples R China.;[Kuang, Guang-Wei; Chen, Fu-Hua; Long, Xue-Ming] Inst Hunan Prov Vet Drugs & Feed Supervis, Changsha 410006, Hunan, Peoples R China.
通讯机构：
[Sun, ZL; Liu, ZY] H;Hunan Agr Univ, Coll Vet Med, Hunan Engn Res Ctr Vet Drug, Changsha 410128, Hunan, Peoples R China.
关键词：
Baclofen;Residue;LC-MS/MS;Validation method;Edible animal tissues
摘要：
Baclofen was illegally used in veterinary clinical medicine as a growth-promoting agent. To date, few methods have been developed for the monitoring of baclofen in animal tissues. In this study, a sensitive and efficient liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to identify and quantify baclofen in the muscle, liver, kidney, and fat of swine was developed and validated. Baclofen was extracted from tissues with ammonium acetate buffer (pH 5.2) and isolated with isopropyl-ethyl acetate (4:6, v/v). Then, a solid phase extraction using MCX cartridge was used to clean up the extracts. The elution was evaporated to dryness and reconstituted with water/methanol (90:10 v/v). All samples were determined by LC-MS/MS system through positive ionization in a multiple reaction monitoring (MRM) mode. The proposed method was validated by evaluation of specificity, linearity, recovery, accuracy, precision, LOD, and LOQ values according to Commission Decision 2002/657/EC. Estimated limit of quantification for baclofen in the muscle, liver, kidney, and fat of this method was 1.00 μg/kg, respectively. The mean intra- and inter-day assay accuracies fell within a range 88.5–93.9% and 86.2–93.2%, respectively. The mean intra- and inter-day precisions were 1.78 and 4.95% (RSD < 15%), respectively. The proposed method has proved to be suitable for accurate quantitative determination of baclofen for residue analysis.<br/> ©2017, Springer Science+Business Media, LLC.
语种：
英文
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