喜旱莲子草生物防治研究进展
作者：
余国梁;李政;李健;管桂萍;杨华
期刊：
湖南生态科学学报 ,2020年7(04):62-67 ISSN：2095-7300
作者机构：
湖南农业大学 生物科学技术学院,湖南 长沙410128;[杨华; 管桂萍; 余国梁; 李健; 李政] 湖南农业大学
关键词：
喜旱莲子草;生物防治;微生物防治;天敌昆虫防治;替代控制
摘要：
喜旱莲子草是一种世界性入侵杂草,在我国蔓延严重,对农业、经济、环境都造成了巨大破坏.本文总结了国内外关于喜旱莲子草生物防治的研究进展,主要包括微生物防治、天敌昆虫防治、替代控制等生物防治喜旱莲子草的研究进展和现状.并探讨了今后研究方向,为喜旱莲子草生物防治开发利用提供参考.
语种：
中文
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Pseudomonas species isolated from tobacco seed promote root growth and reduce lead contents in Nicotiana tobacum K326.
作者：
Li, Juan;Zheng, Bufan;Hu, Ruiwen;Liu, Yongjun;Jing, Yongfeng;...
期刊：
CANADIAN JOURNAL OF MICROBIOLOGY ,2019年65(3):214-223 ISSN：0008-4166
通讯作者：
Chen, Wu;Zhou, Qingming
作者机构：
[Zhou, Qingming; Zheng, Bufan; Hu, Ruiwen; Chen, Wu; Chen, W; Zhou, QM; Xiao, Yunhua; Sun, Min; Li, Juan] Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.;[Zhou, Qingming; Zheng, Bufan; Hu, Ruiwen; Chen, Wu; Chen, W; Zhou, QM; Xiao, Yunhua; Sun, Min; Li, Juan] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Zhou, Qingming; Zheng, Bufan; Hu, Ruiwen; Chen, Wu; Chen, W; Zhou, QM; Xiao, Yunhua; Sun, Min; Li, Juan] Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.;[Liu, Yongjun] Inst Hunan Prov Tobacco Sci Res, Changsha 410004, Hunan, Peoples R China.;[Jing, Yongfeng] China Tobacco Hunan Ind Co Ltd, Changsha 410019, Hunan, Peoples R China.
通讯机构：
[Chen, W; Zhou, QM] H;Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.
关键词：
captation du plomb;croissance racinaire;endophytes;lead uptake;root growth
摘要：
Endophytic bacteria are generally helpful for plant growth and protection. We isolated from tobacco seeds three Pseudomonas strains (K03, Y04, and N05) that could produce siderophores, indole-3-acetic acid, and 1-aminocyclopropane-1-carboxylate deaminase, fix nitrogen, dissolve phosphorus and potassium, and tolerate heavy metals. In pot experiments, the three isolated strains significantly promoted root growth and increased the root enzyme activity in Nicotiana tobacum K326. Furthermore, bacterial inoculations increased the proportion of residual lead (Pb) by 8.36%–51.63% and decreased the total Pb content by 3.28%–6.38% in the contaminated soil during tobacco planting, compared with uninoculated soils. An effective decrease in Pb content was also found in tobacco leaves with bacterial inoculations. K03 inoculation decreased the Pb content in the upper leaves by 49.80%, and Y04 inoculation had the best effect, decreasing the Pb content in the middle leaves by 70.12%. Additionally, soil pH and root activity had significant effects on transformation and translocation of Pb. The study suggested that in response to Pb pollution in soil, a reasonable application of endophytes (e.g., Pseudomonas) might be a promising approach in promoting tobacco growth and reducing Pb content in tobacco, while simultaneously enhancing Pb stabilization in soils. © 2019, Published by NRC Research Press.
语种：
英文
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Construction of a multicontrol sterility system for a maize male-sterile line and hybrid seed production based on the ZmMs7 gene encoding a PHD-finger transcription factor
作者：
Zhang, Danfeng;Wu, Suowei;An, Xueli;Xie, Ke;Dong, Zhenying;...
期刊：
Plant Biotechnology Journal ,2018年16(2):459-471 ISSN：1467-7644
通讯作者：
Zhao, J.
作者机构：
[Zhang, Danfeng; Wan, Xiangyuan; Liu, Shuangshuang; Rao, Liqun] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China.;[Zhang, Danfeng; Dong, Zhenying; Wan, Xiangyuan; Xie, Ke; An, Xueli; Zhu, Taotao; Wu, Suowei] Univ Sci & Technol Beijing, Sch Chem & Biol Engn, Adv Biotechnol & Applicat Res Ctr, Beijing, Peoples R China.;[Zhao, Jiuran; Xu, Liwen] Beijing Acad Agr & Forestry Sci, Maize Res Ctr, Beijing Key Lab Maize DNA Fingerprinting & Mol Br, Beijing, Peoples R China.;[Zhang, Danfeng; Zhou, Yan; Fang, Wen; Liu, Shensi; Liu, Shuangshuang; Li, Jinping] Beijing Solidwill Sci Tech Co Ltd, Beijing Engn Lab Main Crop Biotechnol Breeding, Beijing, Peoples R China.
通讯机构：
Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Maize Research Center, Beijing Academy of Agriculture & Forestry Sciences, Beijing, China
关键词：
ZmMs7;genetic male sterility;hybrid seed production;maize;nontransgenic progeny;transgenic maintainer line
摘要：
Although hundreds of genetic male sterility (GMS) mutants have been identified in maize, few are commercially used due to a lack of effective methods to produce large quantities of pure male-sterile seeds. Here, we develop a multicontrol sterility (MCS) system based on the maize male sterility 7 (ms7) mutant and its wild-type Zea mays Male sterility 7 (ZmMs7) gene via a transgenic strategy, leading to the utilization of GMS in hybrid seed production. ZmMs7 is isolated by a map-based cloning approach and encodes a PHD-finger transcription factor orthologous to rice PTC1 and Arabidopsis MS1. The MCS transgenic maintainer lines are developed based on the ms7-6007 mutant transformed with MCS constructs containing the (i) ZmMs7 gene to restore fertility, (ii) α-amylase gene ZmAA and/or (iii) DNA adenine methylase gene Dam to devitalize transgenic pollen, (iv) red fluorescence protein gene DsRed2 or mCherry to mark transgenic seeds and (v) herbicide-resistant gene Bar for transgenic seed selection. Self-pollination of the MCS transgenic maintainer line produces transgenic red fluorescent seeds and nontransgenic normal colour seeds at a 1:1 ratio. Among them, all the fluorescent seeds are male fertile, but the seeds with a normal colour are male sterile. Cross-pollination of the transgenic plants to male-sterile plants propagates male-sterile seeds with high purity. Moreover, the transgene transmission rate through pollen of transgenic plants harbouring two pollen-disrupted genes is lower than that containing one pollen-disrupted gene. The MCS system has great potential to enhance the efficiency of maize male-sterile line propagation and commercial hybrid seed production. © 2017 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.
语种：
英文
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The role of soil bacterial community during winter fallow period in the incidence of tobacco bacterial wilt disease
作者：
Xiao, Yunhua;Liu, Xueduan;Meng, Delong;Tao, Jiemeng;Gu, Yabing;...
期刊：
Applied Microbiology and Biotechnology ,2018年102(5):2399-2412 ISSN：0175-7598
通讯作者：
Li, Juan;Yin, Huaqun
作者机构：
[Xiao, Yunhua; Li, Juan] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Xiao, Yunhua; Li, Juan] Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.;[Meng, Delong; Liu, Xueduan; Yin, Huaqun; Xiao, Yunhua; Tao, Jiemeng; Gu, Yabing] Cent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Hunan, Peoples R China.
通讯机构：
[Li, Juan] H;[Yin, Huaqun] C;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.;Cent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Hunan, Peoples R China.
关键词：
Crop morbidity;Biological control agents;Bacterial interactions;Bacterial diversity;Bacterial community structure
摘要：
Bacterial wilt, caused by Ralstonia solanacearum, occurs occasionally during tobacco planting and potentially brings huge economic losses in affected areas. Soil microbes in different management stages play important roles in influencing bacterial wilt incidence. Studies have focused on the impacts of species diversity and composition during cropping periods on disease morbidity; however, the effects of the soil bacterial biomass, species diversity, species succession, and population interactions on morbidity remain unclear during non-cropping periods. In this study, we explored the soil bacterial communities in the non-cropping winter fallow (WF) and cropping late growing (LG) periods under consecutive monoculture systems using 16S ribosomal RNA gene sequencing and qPCR and further analyzed their effects on tobacco bacterial wilt incidence. We found that the bacterial communities in the WF period were significantly different from those in the LG period based on detrended correspondence analysis and dissimilarity tests. Crop morbidity was significantly related to bacterial community structure and to the presence of some genera during WF and LG periods. These genera, such as Arthrobacter, Pseudomonas, Acidobacteria GP6, and Pasteuria, may be potential biological control agents for bacterial wilt. Further analysis indicated that low soil bacterial diversity during the WF period, decrease of bacterial interactions from the WF to LG periods, and low soil biomass during the LG period all have the potential to increase morbidity. In conclusion, an increase of soil bacterial diversity and control of some bacterial abundances in the WF period might be an effective approach in controlling bacterial wilt incidence. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.
语种：
英文
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The SnRK2 kinases modulate miRNA accumulation in Arabidopsis
作者：
Yan, Jun;Wang, Pengcheng;Wang, Bangshing;Hsu, Chuan-Chih;Tang, Kai;...
期刊：
PLOS GENETICS ,2017年13(4):e1006753 ISSN：1553-7404
通讯作者：
Zhu, Jian-Kang
作者机构：
[Zhu, Jian-Kang; Zhu, Xiaohong; Zhao, Chunzhao; Li, Jianming; Zhao, Yang; Yan, Jun; Wang, Pengcheng] Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, Shanghai, Peoples R China.;[Zhu, Jian-Kang; Zhu, Xiaohong; Zhao, Chunzhao; Li, Jianming; Zhao, Yang; Yan, Jun; Wang, Pengcheng] Chinese Acad Sci, Ctr Excellence Mol Plant Sci, Shanghai, Peoples R China.;[Zhu, Jian-Kang; Zhang, Hairong; Wang, Qiming; Tang, Kai; Zhu, Xiaohong; Zhao, Chunzhao; Wang, Bangshing; Zhao, Yang; Yan, Jun; Hou, Yueh-Ju; Wang, Pengcheng] Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47907 USA.;[Hsu, Chuan-Chih; Tao, W. Andy] Purdue Univ, Dept Biochem, W Lafayette, IN 47907 USA.;[Zhang, Hairong] Henan Agr Univ, Coll Life Sci, State Key Lab Wheat & Maize Crop Sci, Zhengzhou, Peoples R China.
通讯机构：
[Zhu, Jian-Kang] C;[Zhu, Jian-Kang] P;Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, Shanghai, Peoples R China.;Chinese Acad Sci, Ctr Excellence Mol Plant Sci, Shanghai, Peoples R China.;Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47907 USA.
关键词：
MicroRNAs;Phosphorylation;Biosynthesis;Arabidopsis thaliana;Seedlings;Leaves;Protein abundance;In vitro kinase assay
摘要：
MicroRNAs (miRNAs) regulate gene expression and play critical roles in growth and development as well as stress responses in eukaryotes. miRNA biogenesis in plants requires a processing complex that consists of the core components DICER-LIKE 1 (DCL1), SERRATE (SE) and HYPONASTIC LEAVES (HYL1). Here we show that inactivation of functionally redundant members of the SnRK2 kinases, which are the core components of abscisic acid (ABA) and osmotic stress signaling pathways, leads to reduction in miRNA accumulation under stress conditions. Further analysis revealed that the steady state level of HYL1 protein in plants under osmotic stress is dependent on the SnRK2 kinases. Additionally, our results suggest that the SnRK2 kinases physically associate with the miRNA processing components SE and HYL1 and can phosphorylate these proteins in vitro. These findings reveal an important role for the SnRK2 kinases in the regulation of miRNA accumulation and establish a mechanism by which ABA and osmotic stress signaling is linked to miRNA biogenesis. © 2017 Yan et al.
语种：
英文
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借助科研平台开展基因工程实验课教学改革探索与实践
作者：
赵燕;黄丽华;张学文;李健
期刊：
现代农业科技 ,2017年(24):276-278 ISSN：1007-5739
作者机构：
湖南农业大学生物科学技术学院,湖南长沙,410128
关键词：
科研平台;基因工程;实验课程;教学改革;成效;建议
摘要：
基因工程实验是基因工程课程教学的重要环节.本文基于对基因工程实验教学内容的整合和优化,借助科研平台优势进行基因工程实验教学改革探索与实践,分析了教学改革成效与存在的不足,并提出相应建议,以期调动学生的学习兴趣、培养学生的科研素质、提高基因工程实验课程教学质量.
语种：
中文
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Cloning and functional analysis of MADS-box genes, TaAG-A and TaAG-B, from a wheat K-type cytoplasmic male sterile line
作者：
Yang, Wenlong* ;Lou, Xueyuan;Li, Juan;Pu, Mingyu;Mirbahar, Ameer A.;...
期刊：
FRONTIERS IN PLANT SCIENCE ,2017年8:1081 ISSN：1664-462X
通讯作者：
Yang, Wenlong;Zhang, Aimin
作者机构：
[Sun, Jiazhu; Mirbahar, Ameer A.; Yang, Wenlong; Lou, Xueyuan; Zhang, Aimin; Liu, Dongcheng; Pu, Mingyu] Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing, Peoples R China.;[Lou, Xueyuan] Henan Agr Univ, Coll Forestry, Zhengzhou, Peoples R China.;[Li, Juan; He, Lixiong] Hunan Agr Univ, Coll Life Sci, Changsha, Hunan, Peoples R China.;[Mirbahar, Ameer A.] Shah Abdul Latif Univ, Dept Bot, Khairpur, Pakistan.;[Zhang, Aimin; Zhan, Kehui] Henan Agr Univ, Collaborat Ctr Grain Crops Henan, Coll Agron, Zhengzhou, Peoples R China.
通讯机构：
[Yang, WL; Zhang, AM] C;[Zhang, Aimin] H;Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing, Peoples R China.;Henan Agr Univ, Collaborat Ctr Grain Crops Henan, Coll Agron, Zhengzhou, Peoples R China.
关键词：
MADS-box gene;cloning;functional analysis;male sterile;wheat
摘要：
Wheat (Triticum aestivum L.) is a major crop worldwide. The utilization of heterosis is a promising approach to improve the yield and quality of wheat. Although there have been many studies on wheat cytoplasmic male sterility, its mechanism remains unclear. In this study, we identified two MADS-box genes from a wheat K-type cytoplasmic male sterile (CMS) line using homology-based cloning. These genes were localized on wheat chromosomes 3A and 3B and named TaAG-A and TaAG-B, respectively. Analysis of TaAG-A and TaAG-B expression patterns in leaves, spikes, roots, and stems of Chinese Spring wheat determined using quantitative RT-PCR revealed different expression levels in different tissues. TaAG-A had relatively high expression levels in leaves and spikes, but low levels in roots, while TaAG-B had relatively high expression levels in spikes and lower expression in roots, stems, and leaves. Both genes showed downregulation during the mononucleate to trinucleate stages of pollen development in the maintainer line. In contrast, upregulation of TaAG-B was observed in the CMS line. The transcript levels of the two genes were clearly higher in the CMS line compared to the maintainer line at the trinucleate stage. Overexpression of TaAG-A and TaAG-B in Arabidopsis resulted in phenotypes with earlier reproductive development, premature mortality, and abnormal buds, stamens, and stigmas. Overexpression of TaAG-A and TaAG-B gives rise to mutants with many deformities. Silencing TaAG-A and TaAG-B in a fertile wheat line using the virus-induced gene silencing (VIGS) method resulted in plants with green and yellow striped leaves, emaciated spikes, and decreased selfing seed set rates. These results demonstrate that TaAG-A and TaAG-B may play a role in male sterility in the wheat CMS line. © 2017 Yang, Lou, Li, Pu, Mirbahar, Liu, Sun, Zhan, He and Zhang.
语种：
英文
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Quantitative proteomic analysis of upland cotton stem terminal buds reveals phytohormone-related pathways associated with dwarfism
作者：
Tu, X.;Li, J.;Wang, Q.* ;Liu, A.*
期刊：
BIOLOGIA PLANTARUM ,2017年61(1):106-114 ISSN：0006-3134
通讯作者：
Liu, A.;Wang, Q.
作者机构：
[Tu, X.] Hunan Agr Univ, Coll Orient Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Liu, A.; Tu, X.; Li, J.] Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.;[Wang, Q.] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liu, A.; Wang, Q.] H;Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
关键词：
auxins;cytokinins;gibberellins;Gossypium hirsutum;iTRAQ;near-isogenic line
摘要：
Identifying important regulative elements and pathways related to dwarfism in cotton is a major challenge in cotton breeding. Isobaric tags for relative and absolute quantitation-based proteomics of stem terminal buds from upland cotton (Gossypium hirsutum L.) dwarf line LA-1 and high near-isogenic line LH-1 was performed. Moreover, transcriptional expression of differentially-expressed proteins (DEPs) belonging to phytohormone signal transduction, hormone biosynthesis, and ubiquitin system were analysed using quantitative real-time polymerase chain reaction. A total of 4 849 proteins were identified from LA-1 and LH-1, 697 of which showed differential accumulations. Most of the DEPs have catalytic, binding, and transporter activity and are involved in metabolism- and protein processing-related pathways. In particular, seven DEPs, including two gibberellin (GA) receptors, three cytokinin (CK) receptors, CKoxidase, and CK-N-glucosyltransferase were up-regulated in LA-1, and GA20-oxidase was down-regulated in LH-1. Our results suggest that the DELLA-independent GAsignalling pathway is the primary cause of dwarfism in LA-1 and indicate that CKresponse element 1-2, GA-insensitive dwarf, CKoxidase, and GA3-β-dioxygenase are potential indicators of dwarf cotton. The profiling of DEPs may offer a valuable resource for cotton breeding. © 2017, Springer Science+Business Media Dordrecht.
语种：
英文
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遗传学实验课程教学内容的优化与实施
作者：
赵燕;黄丽华;李健
期刊：
现代农业科技 ,2016年(19):295-296,299 ISSN：1007-5739
作者机构：
湖南农业大学生物科学技术学院,湖南长沙,410128
关键词：
遗传学;实验教学;优化与实施;综合设计性实验
摘要：
针对传统遗传学实验教学中存在的问题,在教学中确立注重基础、加强能力、提高素质的全面发展教育观,对遗传学的实验教学内容加以优化,设置模块式课程实验和综合实验,结合教学方法及教学组织形式上的改革,重点突出实验的设计性、综合性及创新性,实施全天性开放实验教学,着力培养学生的研究性学习能力,拓展了学生的知识视野,提高了遗传学实验课程教学质量。
语种：
中文
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Identification of stable quantitative trait loci (QTLs) for fiber quality traits across multiple environments in Gossypium hirsutum recombinant inbred line population
作者：
Jamshed, Muhammad;Jia, Fei;Gong, Juwu;Palanga, Koffi Kibalou;Shi, Yuzhen;...
期刊：
BMC Genomics ,2016年17(1):1-13 ISSN：1471-2164
通讯作者：
Gong, Wankui;Yuan, Youlu
作者机构：
[Palanga, Koffi Kibalou; Tan, Yunna; Yuan, Youlu; Deng, Xiaoying; Gong, WK; Yuan, YL; Zhang, Zhen; Chen, Tingting; Shi, Yuzhen; Li, Junwen; Liu, Aiying; Gong, Wankui; Rashid, Harun Or; Sarfraz, Zareen; Jia, Fei; Cai, Juan; Jamshed, Muhammad; Gong, Juwu; Ge, Qun; Shang, Haihong] Chinese Acad Agr Sci, Key Lab Biol & Genet Breeding Cotton, Minist Agr, Inst Cotton Res,State Key Lab Cotton Biol, Anyang 455000, Henan, Peoples R China.;[Gong, Juwu] Xinjiang Agr Univ, Coll Agron, Key Lab Agrobiotechnol, Urumqi 830052, Xinjiang, Peoples R China.;[Liu, Zhi] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Lu, Quanwei] Anyang Coll Technol, Anyang 455000, Henan, Peoples R China.;[Hassan, Murtaza] Peking Univ, Coll Engn, Dept Mat Sci & Engn, Beijing 100871, Peoples R China.
通讯机构：
[Gong, WK; Yuan, YL] C;Chinese Acad Agr Sci, Key Lab Biol & Genet Breeding Cotton, Minist Agr, Inst Cotton Res,State Key Lab Cotton Biol, Anyang 455000, Henan, Peoples R China.
关键词：
Recombinant inbred line;Upland cotton;Multiple environments;SSR markers;Meta-QTL analyses;Stable QTLs
摘要：
Background: The identification of quantitative trait loci (QTLs) that are stable and consistent across multiple environments and populations plays an essential role in marker-assisted selection (MAS). In the present study, we used 28,861 simple sequence repeat (SSR) markers, which included 12,560 Gossypium raimondii (D genome) sequence-based SSR markers to identify polymorphism between two upland cotton strains 0-153 and sGK9708. A total of 851 polymorphic primers were finally selected and used to genotype 196 recombinant inbred lines (RIL) derived from a cross between 0 and 153 and sGK9708 and used to construct a linkage map. The RIL population was evaluated for fiber quality traits in six locations in China for five years. Stable QTLs identified in this intraspecific cross could be used in future cotton breeding program and with fewer obstacles. Results: The map covered a distance of 4,110 cM, which represents about 93.2 % of the upland cotton genome, and with an average distance of 5.2 cM between adjacent markers. We identified 165 QTLs for fiber quality traits, of which 47 QTLs were determined to be stable across multiple environments. Most of these QTLs aggregated into clusters with two or more traits. A total of 30 QTL clusters were identified which consisted of 103 QTLs. Sixteen clusters in the At sub-genome comprised 44 QTLs, whereas 14 clusters in the Dt sub-genome that included 59 QTLs for fiber quality were identified. Four chromosomes, including chromosome 4 (c4), c7, c14, and c25 were rich in clusters harboring 5, 4, 5, and 6 clusters respectively. A meta-analysis was performed using Biomercator V4.2 to integrate QTLs from 11 environmental datasets on the RIL populations of the above mentioned parents and previous QTL reports. Among the 165 identified QTLs, 90 were identified as common QTLs, whereas the remaining 75 QTLs were determined to be novel QTLs. The broad sense heritability estimates of fiber quality traits were high for fiber length (0.93), fiber strength (0.92), fiber micronaire (0.85), and fiber uniformity (0.80), but low for fiber elongation (0.27). Meta-clusters on c4, c7, c14 and c25 were identified as stable QTL clusters and were considered more valuable in MAS for the improvement of fiber quality of upland cotton.Conclusion: Multiple environmental evaluations of an intraspecific RIL population were conducted to identify stable QTLs. Meta-QTL analyses identified a common chromosomal region that plays an important role in fiber development. Therefore, QTLs identified in the present study are an ideal candidate for MAS in cotton breeding programs to improve fiber quality. © 2016 Jamshed et al.
语种：
英文
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Improvement of bacterial blight and brown planthopper resistance in an elite restorer line Huazhan of Oryza
作者：
Xiao, Youlun;Li, Jinjiang;Yu, Jianghui;Meng, Qiucheng;Deng, Xiangyang;...
期刊：
Field Crops Research ,2016年186:47-57 ISSN：0378-4290
通讯作者：
Xiao, Guoying
作者机构：
[Xiao, Youlun; Yi, Zili] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Xiao, Youlun; Xiao, Guoying; Li, Jinjiang; Meng, Qiucheng; Yu, Jianghui; Deng, Xiangyang] Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Hunan, Peoples R China.;[Xiao, Youlun] Hunan Acad Agr Sci, Hunan Plant Protect Inst, Changsha 410125, Hunan, Peoples R China.
通讯机构：
[Xiao, Guoying] C;Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Hunan, Peoples R China.
关键词：
Bacterial blight resistance;Brown planthopper resistance;Gene pyramiding;Hybrid rice;Restorer line
摘要：
Development of resistant varieties is one of the most economical and effective strategies to prevent rice from bacterial blight disease (BB) and brown planthopper (BPH), the two main pests jeopardizing rice production. The hybrid rice Tianyouhuazhan (TianfengA/Huazhan) has been widely used in rice production in China, but this hybrid is susceptible to BB and BPH. In this study, one BB resistance gene (Xa23) and two BPH resistance genes (Bph14 and Bph15) were simultaneously introgressed into the restorer line Huazhan to improve the BB and BPH resistance of Tianyouhuazhan using marker-assisted backcrossing (MABC) strategy coupled with phenotypic selection. The results of identification of BB and BPH resistance revealed that almost all of the improved restorer lines and their derived hybrids showed high resistance (HR) or resistance (R) to BB and BPH. Almost all of the agronomic traits of improved restorer lines and their hybrids were similar to those of their respective original versions in field trial, except the 1000-grain weight of improved hybrids showed an extremely significant increase than that of Tianyouhuazhan. Plot yields test in 2013 and 2014 suggested that two improved hybrid combinations named as TianfengA/R43-06 and TianfengA/R43-07 had higher grain yields per plot than Tianyouhuazhan and other improved hybrids. These results clearly indicate that pyramiding of Xa23, Bph14 and Bph15 genes is a useful approach for improving BB and BPH resistance, two hybrid combinations TianfengA/R43-06 and TianfengA/R43-07 could replace Tianyouhuazhan for extension in BB and/or BPH epidemic area in China. © 2015 Elsevier B.V.
语种：
英文
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Quantitative trait loci analysis of Verticillium wilt resistance in interspecific backcross populations of Gossypium hirsutum × Gossypium barbadense
作者：
Shi, Yuzhen;Zhang, Baocai;Liu, Aiying;Li, Wentan;Li, Junwen;...
期刊：
BMC Genomics ,2016年17(1):1-15 ISSN：1471-2164
通讯作者：
Yuan, Youlu
作者机构：
[Li, Wentan; Yuan, Youlu; Lu, Quanwei; Zhu, Heqin; Zhang, Zhen; Chen, Tingting; Shi, Yuzhen; Li, Junwen; Wang, Tao; Liu, Aiying; Li, Shaoqi; Gong, Wankui; Gong, Juwu; Ge, Qun; Zhang, Baocai; Shang, Haihong] Chinese Acad Agr Sci, Inst Cotton Res, State Key Lab Cotton Biol, Key Lab Biol & Genet Breeding Cotton,Minist Agr, Anyang 455000, Henan, Peoples R China.;[Liu, Zhi] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Yuan, Youlu] C;Chinese Acad Agr Sci, Inst Cotton Res, State Key Lab Cotton Biol, Key Lab Biol & Genet Breeding Cotton,Minist Agr, Anyang 455000, Henan, Peoples R China.
关键词：
Cotton;Verticillium wilt (VW);Quantitative trait loci (QTL);Interspecific backcross population
摘要：
Background: Verticillium wilt (VW) caused by Verticillium dahliae (Kleb) is one of the most destructive diseases of cotton. The identification of highly resistant QTLs or genes in the whole cotton genome is quite important for developing a VW-resistant variety and for further molecular design breeding. Results: In the present study, BC1F1, BC1S1, and BC2F1 populations derived from an interspecific backcross between the highly resistant line Hai1 (Gossypium barbadense L.) and the susceptible variety CCRI36 (G. hirsutum L.) as the recurrent parent were constructed. Quantitative trait loci (QTL) related to VW resistance were detected in the whole cotton genome using a high-density simple sequence repeat (SSR) genetic linkage map from the BC1F1 population, with 2292 loci covering 5115.16 centiMorgan (cM) of the cotton (AD) genome, and the data concerning VW resistance that were obtained from four dates of BC2F1 in the artificial disease nursery and one date of BC1S1 and BC2F1 in the field. A total of 48 QTLs for VW resistance were identified, and 37 of these QTLs had positive additive effects, which indicated that the G. barbadense alleles increased resistance to VW and decreased the disease index (DI) by about 2.2-10.7. These QTLs were located on 19 chromosomes, in which 33 in the A subgenome and 15 QTLs in the D subgenome. The 6 QTLs were found to be stable. The 6 QTLs were consistent with those identified previously, and another 42 were new, unreported QTLs, of which 31 QTLs were from G. barbadense. By meta-analysis, 17 QTL hotspot regions were identified and 10 of them were new, unreported hotspot regions. 29 QTLs in this paper were in 12 hotspot regions and were all from G. barbadense. Conclusions: These stable or consensus QTL regions warrant further investigation to better understand the genetics and molecular mechanisms underlying VW resistance. This study provides useful information for further comparative analysis and marker-assisted selection in the breeding of disease-resistant cotton. It may also lay an important foundation for gene cloning and further molecular design breeding for the entire cotton genome. © 2016 The Author(s).
语种：
英文
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Constructing a high-density linkage map for Gossypium hirsutum×Gossypium barbadense and identifying QTLs for lint percentage
作者：
Shi, Yuzhen;Li, Wentan;Li, Aiguo;Ge, Ruihua;Zhang, Baocai;...
期刊：
植物学报：英文版 ,2015年57(5):450-467 ISSN：1672-9072
通讯作者：
Yuan, Youlu
作者机构：
[Li, Wentan; Yuan, Youlu; Wang, Wei; Ge, Ruihua; Chen, Tingting; Wang, Kunbo; Shi, Yuzhen; Liu, Guangping; Li, Junwen; Wang, Tao; Li, Junzhi; Liu, Aiying; Gong, Wankui; Li, Aiguo; Gong, Juwu; Zhang, Baocai; Shang, Haihong; Yang, Zemao] Chinese Acad Agr Sci, Key Lab Biol & Genet Breeding Cotton, State Key Lab Cotton Biol, Minist Agr,Inst Cotton Res, Anyang 455000, Peoples R China.;[Li, Wentan; Tang, Feiyu] Jiangxi Agr Univ, Coll Agr, Nanchang 330045, Peoples R China.;[Li, Aiguo] Acad Agr Sci Luohe City Henan Prov, Luohe 462000, Peoples R China.;[Liu, Zhi; Ge, Ruihua; Liu, Guangping; Jiang, Jianxiong; Li, Junzhi; Zhu, Weiping; Yang, Zemao] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Yu, Xiaonan] Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Yuan, Youlu] C;Chinese Acad Agr Sci, Key Lab Biol & Genet Breeding Cotton, State Key Lab Cotton Biol, Minist Agr,Inst Cotton Res, Anyang 455000, Peoples R China.
关键词：
Cotton (Gossypium spp.);genetic linkage map;Gossypium barbadense;lint percentage;simple sequence repeat
摘要：
To introgress the good fiber quality and yield from Gossypium barbadense into a commercial Upland cotton variety, a high-density simple sequence repeat (SSR) genetic linkage map was developed from a BC1F1 population of Gossypium hirsutum×Gossypium barbadense. The map comprised 2,292 loci and covered 5115.16 centiMorgan (cM) of the cotton AD genome, with an average marker interval of 2.23 cM. Of the marker order for 1,577 common loci on this new map, 90.36% agrees well with the marker order on the D genome sequence genetic map. Compared with five published high-density SSR genetic maps, 53.14% of marker loci were newly discovered in this map. Twenty-six quantitative trait loci (QTLs) for lint percentage (LP) were identified on nine chromosomes. Nine stable or common QTLs could be used for marker-assisted selection. Fifty percent of the QTLs were from G. barbadense and increased LP by 1.07%-2.41%. These results indicated that the map could be used for screening chromosome substitution segments from G. barbadense in the Upland cotton background, identifying QTLs or genes from G. barbadense, and further developing the gene pyramiding effect for improving fiber yield and quality. © 2014 Institute of Botany, Chinese Academy of Sciences.
语种：
英文
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Specific adaptation of Ustilaginoidea virens in occupying host florets revealed by comparative and functional genomics
作者：
Zhang, Yong;Zhang, Kang;Fang, Anfei;Han, Yanqing;Yang, Jun;...
期刊：
Nature Communications ,2014年5(1):3849 ISSN：2041-1723
通讯作者：
Sun, Wenxian
作者机构：
[Zhou, Ligang; Fang, Anfei; Sun, Wenxian; Zhang, Kang; Yang, Jun; Han, Yanqing; Peng, You-Liang; Xue, Minfeng; Li, Jianqiang; Zhang, Yong] China Agr Univ, Key Lab Plant Pathol, Minist Agr, Dept Plant Pathol, Beijing 100193, Peoples R China.;[Yang, Jun; Peng, You-Liang; Xue, Minfeng] China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China.;[Bao, Jiandong; Zhou, Bo] Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, State Key Lab Breeding Base Zhejiang Sustainable, Hangzhou 310021, Zhejiang, Peoples R China.;[Hu, Dongwei] Zhejiang Univ, Inst Biotechnol, Hangzhou 310058, Zhejiang, Peoples R China.;[Zhou, Bo] Int Rice Res Inst, Manila 1301, Philippines.
通讯机构：
[Sun, Wenxian] C;China Agr Univ, Key Lab Plant Pathol, Minist Agr, Dept Plant Pathol, Beijing 100193, Peoples R China.
摘要：
Ustilaginoidea virens (Cooke) Takah is an ascomycetous fungus that causes rice false smut, a devastating emerging disease worldwide. Here we report a 39.4a €‰Mb draft genome sequence of U. virens that encodes 8,426 predicted genes. The genome has ∼25% repetitive sequences that have been affected by repeat-induced point mutations. Evolutionarily, U. virens is close to the entomopathogenic Metarhizium spp., suggesting potential host jumping across kingdoms. U. virens possesses reduced gene inventories for polysaccharide degradation, nutrient uptake and secondary metabolism, which may result from adaptations to the specific floret infection and biotrophic lifestyles. Consistent with their potential roles in pathogenicity, genes for secreted proteins and secondary metabolism and the pathogen-host interaction database genes are highly enriched in the transcriptome during early infection. We further show that 18 candidate effectors can suppress plant hypersensitive responses. Together, our analyses offer new insights into molecular mechanisms of evolution, biotrophy and pathogenesis of U. virens. © 2014 Macmillan Publishers Limited. All rights reserved.
语种：
英文
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Phylogenetic analysis of the gut bacterial microflora of the fungus-growing termite Macrotermes barneyi
作者：
Zhu, Yunhua;Li, Jian;Liu, Huhu;Yang, Hui;Xin, Sheng;...
期刊：
African Journal of Microbiology Research ,2012年6(9):2071-2078 ISSN：1996-0808
通讯作者：
Tian, Yun
作者机构：
[Zhao, Fei; Tian, Yun; Liu, Huhu; Lu, Xiangyang; Xin, Sheng; Zhang, Xuejia; Li, Jian; Zhu, Yunhua; Yang, Hui] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Zhao, Fei; Tian, Yun; Liu, Huhu; Lu, Xiangyang; Xin, Sheng; Zhang, Xuejia; Li, Jian; Zhu, Yunhua; Yang, Hui] Hunan Agr Bioengn Res Inst, Changsha 410128, Hunan, Peoples R China.;[Zhu, Yunhua] Nanhua Univ, Coll Pharm & Life Sci, Hengyang 421001, Peoples R China.
通讯机构：
[Tian, Yun] H;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
关键词：
Termite;bacteria;symbiosis;restriction;fragment;length;polymorphism.
摘要：
Termites are an extremely successful group of wood-degrading organisms and are therefore important both for their roles in carbon turnover in the environment and as potential sources of biochemical catalysts for efforts aimed at converting wood into biofuels. To contribute to the evolutional study of termite digestive symbiosis, a bacterial 16S rRNA gene clone library from the gut microbial community of the fungus-growing termite Macrotermes barneyi was constructed. After screening by restriction fragment length polymorphism (RFLP) analysis, 25 out of 105 clones with unique RFLP patters were sequenced and phylogenetically analyzed. Many of the clones (95%) were derived from three phyla within the domain bacteria: Bacteroidetes, Firmicutes and Proteobacteria. In addition, a few clones derived from Deferribacteres, Actinobacteria and Planctomycetes were also found. No one clone affiliated with the phylum Spirochaetes was identified, in contrast to the case of wood-feeding termites. The phylogenetic analysis revealed that nearly half of the representative clones (11 phylotypes) formed monophyletic clusters with clones obtained from other termite species, especially with the sequences retrieved from fungus-growing termites. These results indicate that the presence of termite-specific bacterial lineages implies a coevolutional relationship of gut microbes and host termites. The remaining 14 clones formed a cluster, and there was very low sequence similarity (30 to 40%) to known 16S rRNA sequences. The 16S rRNA gene sequence data showed that the majority of the intestinal microflora of M. barneyi consisted of new, uncultured species previously unknown to microbiologists.
语种：
英文
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Plant hormone jasmonate prioritizes defense over growth by interfering with gibberellin signaling cascade
作者：
Yang, Dong-Lei;Yao, Jian;Mei, Chuan-Sheng;Tong, Xiao-Hong;Zeng, Long-Jun;...
期刊：
Proceedings of the National Academy of Sciences of the United States of America ,2012年109(19):E1192-E1200 ISSN：0027-8424
通讯作者：
Yang, Yinong
作者机构：
[He, Zuhua; Tong, Xiao-Hong; Zeng, Long-Jun; Li, Qun; Yang, Dong-Lei] Chinese Acad Sci, Natl Key Lab Plant Mol Genet, Inst Plant Physiol & Ecol, Shanghai Inst Biol Sci, Shanghai 200032, Peoples R China.;[Yang, Yinong; Mei, Chuan-Sheng] Univ Arkansas, Dept Plant Pathol, Fayetteville, AR 72701 USA.;[He, Sheng Yang; Yao, Jian; Thomashow, Michael F.; Lee, Chin Mei] Michigan State Univ, Dept Energy Plant Res Lab, E Lansing, MI 48824 USA.;[He, Sheng Yang; Yao, Jian] Howard Hughes Med Inst, Chevy Chase, MD 20815 USA.;[Xiao, Lang-Tao] Hunan Agr Univ, Hunan Prov Key Lab Phytohormones, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Yang, Yinong] U;Univ Arkansas, Dept Plant Pathol, Fayetteville, AR 72701 USA.
关键词：
disease resistance;plant growth;plant immunity;light response;plant defense
摘要：
Plants must effectively defend against biotic and abiotic stresses to survive in nature. However, this defense is costly and is often accompanied by significant growth inhibition. How plants coordinate the fluctuating growth-defense dynamics is not well understood and remains a fundamental question. Jasmonate (JA) and gibberellic acid (GA) are important plant hormones that mediate defense and growth, respectively. Binding of bioactive JA or GA ligands to cognate receptors leads to proteasome-dependent degradation of specific transcriptional repressors (the JAZ or DELLA family of proteins), which, at the resting state, represses cognate transcription factors involved in defense (e.g., MYCs) or growth [e.g. phytochrome interacting factors (PIFs)]. In this study, we found that the coi1 JA receptor mutants of rice (a domesticated monocot crop) and Arabidopsis (a model dicot plant) both exhibit hallmark phenotypes of GA-hypersensitive mutants. JA delays GA-mediated DELLA protein degradation, and the della mutant is less sensitive to JA for growth inhibition. Overexpression of a selected group of JAZ repressors in Arabidopsis plants partially phenocopies GA-associated phenotypes of the coi1 mutant, and JAZ9 inhibits RGA (a DELLA protein) interaction with transcription factor PIF3. Importantly, the pif quadruple (pifq) mutant no longer responds to JA-induced growth inhibition, and overexpression of PIF3 could partially overcome JA-induced growth inhibition. Thus, a molecular cascade involving the COI1-JAZ-DELLA-PIF signaling module, by which angiosperm plants prioritize JA-mediated defense over growth, has been elucidated.
语种：
英文
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Expression Profile in Rice Panicle: Insights into Heat Response Mechanism at Reproductive Stage
作者：
Zhang, Xianwen* ;Li, Jiaping;Liu, Ailing;Zou, Jie;Zhou, Xiaoyun;...
期刊：
PLOS ONE ,2012年7(11):e49652- ISSN：1932-6203
通讯作者：
Zhang, Xianwen
作者机构：
[Chen, Xinbo; Liu, Ailing; Zhou, Xiaoyun; Peng, Yan; Xiong, Xingyao; Zhang, Xianwen] Hunan Agr Univ, Key Lab Crop Germplasm Innovat & Utilizat Hunan P, Changsha, Hunan, Peoples R China.
通讯机构：
[Zhang, Xianwen] H;Hunan Agr Univ, Key Lab Crop Germplasm Innovat & Utilizat Hunan P, Changsha, Hunan, Peoples R China.
关键词：
Rice;Panicles;Gene expression;Thermal stresses;Plant resistance to abiotic stress;Microarrays;Heat treatment;Heat shock response
摘要：
Rice at reproductive stage is more sensitive to environmental changes, and little is known about the mechanism of heat response in rice panicle. Here, using rice microarray, we provided a time course gene expression profile of rice panicle at anther developmental stage 8 after 40°C treatment for 0 min, 20 min, 60 min, 2 h, 4 h, and 8 h. The identified differentially expressed genes were mainly involved in transcriptional regulation, transport, cellular homeostasis, and stress response. The predominant transcription factor gene families responsive to heat stress were Hsf, NAC, AP2/ERF, WRKY, MYB, and C2H2. KMC analysis discovered the time-dependent gene expression pattern under heat stress. The motif co-occurrence analysis on the promoters of genes from an early up-regulated cluster showed the important roles of GCC box, HSE, ABRE, and CE3 in response to heat stress. The regulation model central to ROS combined with transcriptome and ROS quantification data in rice panicle indicated the great importance to maintain ROS balance and the existence of wide cross-talk in heat response. The present study increased our understanding of the heat response in rice panicle and provided good candidate genes for crop improvement. © 2012 Zhang et al.
语种：
英文
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Characterization of yeast cells as a microencapsulation wall material by Fourier-transform infrared spectroscopy
作者：
Shi, Guorong* ;Rao, Liqun;Xie, Qingji;Li, Jun;Li, Benxiang;...
期刊：
Vibrational Spectroscopy ,2010年53(2):289-295 ISSN：0924-2031
通讯作者：
Shi, Guorong
作者机构：
[Shi, Guorong; Xiong, Xingyao] Key Lab Hunan Crop Germplasm Innovat & Utilizat, Changsha 410128, Hunan, Peoples R China.;[Li, Jun; Shi, Guorong; Li, Benxiang] Hunan Agr Univ, Coll Sci, Changsha 410128, Hunan, Peoples R China.;[Rao, Liqun] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Xie, Qingji] Hunan Normal Univ, Coll Chem & Chem Engn, Minist Educ China, Key Lab Chem Biol & Tradit Chinese Med Res, Changsha 410081, Hunan, Peoples R China.
通讯机构：
[Shi, Guorong] K;Key Lab Hunan Crop Germplasm Innovat & Utilizat, Changsha 410128, Hunan, Peoples R China.
关键词：
FT-IR spectroscopy;Multivariate analysis;S. cerevisiae;Chlorogenic acid;Microencapsulation
摘要：
The chemically induced structural changes of yeast cells as a wall material for the microencapsulation of chlorogenic acid (CGA) were investigated by the Fourier-transform infrared (FT-IR) spectroscopy. Results indicate that the encapsulation performance of S. cerevisiae cells was largely affected by the composition of the culture medium, and the optimal composition of the culture medium was obtained in terms of the encapsulation yield of CGA. The yeast cells cultured under optimal conditions were subject to chemical treatment by sodium chloride, cetyltrimethylammonium bromide (CTAB), Triton X-100, sodium dodecyl sulfate (SDS) or ethanol. The chemical treatments notably changed the encapsulation properties of S. cerevisiae cells for CGA, with the highest encapsulation yield in the CTAB case. All the chemical treatments decreased the FT-IR absorption bands for proteins and nucleic acids of S. cerevisiae cells but increased those for lipids and fatty acids, and it is thus rather difficult to discuss the relationship between the encapsulation yield and the wall structures derived from the original FT-IR spectra. In contrast, multivariate analyses on the second derivative transformation FT-IR spectra of the yeast cell wall materials indicate that under various culture and chemical treatment conditions, the encapsulation yield of yeast cells for CGA was positively correlated with its IR absorption ratios of 1157 cm<sup>-1</sup> to 1043 cm<sup>-1</sup> (D1157/D1043) and of 1537 cm<sup>-1</sup> to 1043 cm<sup>-1</sup> (D1537/D1043) significantly (p <0.05), whereas negatively correlated with those of 1080 cm<sup>-1</sup> to 1043 cm<sup>-1</sup> (D1080/D1043), 1458 cm<sup>-1</sup> to 1043 cm<sup>-1</sup> (D1458/D1043) and 1740 cm<sup>-1</sup> to 1043 cm <sup>-1</sup> (D1740/D1043) significantly (p <0.05). The five absorption ratios proposed here from the second derivative transformation FT-IR spectra may be a rapid gauge for predicting the encapsulation properties of yeast cells and screening out optimal yeast cells for encapsulation of other species. ©2010 Elsevier B.V. All rights reserved.
语种：
英文
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湖南省10个现行栽培桑树品种的AFLP指纹图谱分析
作者：
黄仁志;颜新培;李健;张学文
期刊：
蚕业科学 ,2009年35(4):837-841 ISSN：0257-4799
作者机构：
[黄仁志] 湖南农业大学生物科学技术学院,长沙,410128;[黄仁志] 湖南省蚕桑科学研究所,长沙,410127;[颜新培] 湖南省蚕桑科学研究所;[张学文; 李健] 湖南农业大学
关键词：
桑树;湖南省推广品种;DNA指纹图谱;扩增长度多态性分子标记
摘要：
利用扩增长度多态性(AFLP)分子标记技术,构建湖南省现行推广的10个桑树品种的指纹图谱,作为该省区桑树遗传育种辅助选择和品种鉴定的重要依据.从36对AFLP引物中筛选出带型丰富、多态性较高的5对引物对10份桑树品种材料进行扩增,共检测到344条多态性条带,多态性比率达27.88%.品种之间的遗传相似系数以及UPGMA聚类分析结果与基于形态学方面的分类结果一致,在遗传相似系数0.79处,10个桑树品种聚为3类:三倍体品种湘桑6号单独聚为一类,其余9个品种聚为一类,其中广东桑品种苗33又单独聚为一类,8个鲁桑品种聚为一类.
语种：
中文
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属间杂交获得的甘蓝型油菜雄性不育材料的研究
作者：
郑卓;李健;钟军;李栒;官春云
期刊：
中国油料作物学报 ,2008年30(2):242-245 ISSN：1007-9084
作者机构：
[郑卓] 井冈山大学生命科学学院;[李健] 湖南农业大学生物科学技术学院;[钟军; 李栒; 官春云] 湖南农业大学油料研究所;湖南农业大学油料研究所 江西吉安343009;湖南长沙410128
关键词：
新疆野生油菜;甘蓝型油菜;远缘杂交;显性核不育
摘要：
采用蕾期剥蕾授粉的方法,成功获得了新疆野生油菜野油2000-1和甘蓝型油菜Parol属间杂种.杂种F1育性极低,自由授粉条件下获得少量F2种子.在F2群体中成功选择到一株雄性不育株,用Parol做轮回亲本与不育株回交,BC_1、BC_2和BC_3群体中可育株与不育株均呈1:1分离.用波里马细胞质雄性不育的保持系和恢复系、681A的保持系和恢复系、隐性核不育86A的保持系及其它33个甘蓝型油菜品系与BC_2群体中的不育株测交,测交后代可育株与不育株呈5:3分离的组合有20个,呈1:1分离的组合有14个,呈3:1分离的组合有3个,有一个组合的后代完全可育,表明该雄性不育材料可能为显性核不育.
语种：
中文
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