转基因油菜抗旱、抗寒及休眠能力的比较
作者：
李健;郑卓;李栒;官春云
期刊：
中国油料作物学报 ,2007年29(2):162-165 ISSN：1007-9084
作者机构：
湖南农业大学生物科学技术学院,湖南,长沙,410128;[郑卓] 井冈山学院生命科学技术系,江西,吉安,334009;湖南农业大学油料作物研究所,湖南,长沙,410128;[李栒; 官春云; 李健] 湖南农业大学
关键词：
转基因油菜;抗旱性;抗寒性;休眠;发芽率
摘要：
以3个转基因油菜品系及3个对照品种为材料,在大田条件下比较研究了转基因油菜与非转基因对照苗期的抗旱能力、抗寒能力及其种子在大田土壤中的休眠能力.结果表明,转基因油菜与非转基因对照苗期都有较好的抗旱能力、抗寒能力,两者之间无显著性差异.在埋入土壤表层(3cm)与深层(20cm)条件下,转基因与非转基因对照种子之间的萌发率差异均不显著.
语种：
中文
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高粱总DNA不同提取方法的比较
作者：
郑卓;李健;圣忠华;彭智勇;罗宝
期刊：
安徽农业科学 ,2007年35(36):11758-11759 ISSN：0517-6611
作者机构：
[郑卓; 圣忠华; 彭智勇; 罗宝] 井冈山大学生命科学学院;[李健] 湖南农业大学生物科学技术学院
关键词：
高粱;尿素法;SDS法;CTAB法;NaOH法
摘要：
[目的]探索高粱总DNA的快速、高效提取方法,为将其导入水稻提供大量、高质量的DNA。[方法]以高粱幼叶为材料,分别采用SDS法、CTAB法、尿素法和NaOH法提取高粱总DNA,并对不同方法提取DNA的纯度、浓度和产量进行检测。[结果]尿素法提取DNA纯度和质量最好,SDS法和CTAB法次之,NaOH法最差。采用尿素法、SDS法、CTAB法和NaOH法分别可从1g高粱样品中提取到约292.00、21.75、152.25和243.75μg的DNA。4种方法提取的高粱总DNA的琼脂糖凝胶电泳结果表明NaOH法不能有效提取高粱总DNA,其他3种方法在提取高粱总DNA时能较好地保证其完整性。高粱总DNA分子量约为50KB。[结论]尿素法提取DNA的纯度最好、产率最高,是一种理想的高粱总DNA提取方法。
语种：
中文
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转基因油菜的外源Barstar基因向近缘种的转移研究
作者：
李健;官春云;李栒;陈社员;郑卓;...
期刊：
湖南农业大学学报(自然科学版) ,2006年32(6):591-595 ISSN：1007-1032
作者机构：
湖南农业大学,生物科学技术学院,湖南,长沙,410128;湖南农业大学,油料作物研究所,湖南,长沙,410128;[郑卓] 井冈山学院,生命科学技术系,江西,吉安,334009;[李栒; 官春云; 陈平平; 陈社员; 李健] 湖南农业大学
关键词：
转基因油菜;基因转移;杂交;聚合酶链式反应
摘要：
在人工杂交与田间自然授粉两种条件下,研究了转Barstar基因油菜T742向非转基因油菜、其他芸薹属、十字花科植物和杂草的基因转移情况.结果表明,转基因油菜与周围非转基因油菜和新疆野生油菜杂交能产生一定数量的种子,经PPT抗性与PCR检测,其中部分种子含有Barstar基因,而与其他十字花科植物或杂草杂交不能得到杂种;基因转移受到母本类型的影响,转基因油菜T742与3种类型油菜的杂交亲合率从高到低为甘蓝型、白菜型、芥菜型.可见转基因油菜的外源Barstar基因可向周围非转基因油菜和新疆野生油菜进行转移,而很难向其他十字花科植物或杂草转移.
语种：
中文
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新疆野生油菜与甘蓝型油菜属间杂交亲和性及杂种分子鉴定
作者：
郑卓;李健;李栒;官春云
期刊：
湖南农业大学学报(自然科学版) ,2006年32(5):465-468 ISSN：1007-1032
作者机构：
井冈山学院生命科学学院;湖南农业大学生物科学技术学院;湖南农业大学油料研究所;湖南农业大学油料研究所 江西吉安343009;湖南农业大学油料研究所 湖南长沙410128
关键词：
新疆野生油菜;甘蓝型油菜;远缘杂交不亲和;蕾期授粉
摘要：
采用蕾期剥蕾授粉的方法,研究了新疆野生油菜与甘蓝型油菜属间杂交亲和性.属间杂种F1植株形态表现一致,介于双亲之间,并且具有明显的杂种优势.从新疆野生油菜×湘油15号F1群体中选取9株进行RAPD分析,扩增出的带型完全一致,并且是父母本随机扩增DNA片段的综合,说明这9株全为真杂种.试验结果表明,蕾期授粉可以克服新疆野生油菜和甘蓝型油菜属间杂交的不亲和性,利用RAPD分子标记技术可以快速有效地鉴定远缘杂种.
语种：
中文
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Immunosensor for rapid detection of gibberellin acid in the rice grain
作者：
Li, J;Xiao, LT;Zeng, GM;Huang, GH;Shen, GL* ;...
期刊：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ,2005年53(5):1348-1353 ISSN：0021-8561
通讯作者：
Shen, GL
作者机构：
[Shen, GL] Hunan Univ, Dept Environm Sci & Engn, Chem & Chem Engn Coll, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China.;Hunan Agr Univ, Key Lab Phytohormones Hunan Prov, Changsha 410028, Peoples R China.
通讯机构：
[Shen, GL] H;Hunan Univ, Dept Environm Sci & Engn, Chem & Chem Engn Coll, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China.
关键词：
immunosensor;gibberellin acid;competitive immunoreaction;copper ion labeled
摘要：
A rapid, selective, sensitive, accurate, and inexpensive immunosensor for gibberellin acid detection was designed by coupling immunoassay with the square wave anodic stripping voltammetry (SWASV) technique involving copper ion labeled antigen in the competitive immunoreaction. The response signal expressed as the percentage of current reduction CR % (y) is linearly related to the concentration of GA (x) in the 1 μg/mL to ∼150 μg/mL range with a regression equation of the form y = 0.44x + 15.59 and a correlation coefficient of 0.99. The results of the immunosensor assay were compared with those obtained by HPLC and ELISA, which show a satisfactory agreement. The immunosensor was used to determine the GA content in the hybrid rice grain samples taken in the growing period. © 2005 American Chemical Society.
语种：
英文
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Amperometric immunosensor based on polypyrrole/poly(m-pheylenediamine) multilayer on glassy carbon electrode for cytokinin N6-(Δ2-isopentenyl) adenosine assay
作者：
Li, J;Xiao, LT;Zeng, GM;Huang, GH;Shen, GL* ;...
期刊：
Analytical Biochemistry ,2003年321(1):89-95 ISSN：0003-2697
通讯作者：
Shen, GL
作者机构：
[Shen, GL] Hunan Univ, Chem & Chem Engn Coll, Dept Environm Sci & Engn, State Key Lab Chem Biosensing & Chemometr, Changsha 410082, Peoples R China.;Hunan Agr Univ, Key Lab Phytohormones Hunan Province, Changsha 410028, Peoples R China.
通讯机构：
[Shen, GL] H;Hunan Univ, Chem & Chem Engn Coll, Dept Environm Sci & Engn, State Key Lab Chem Biosensing & Chemometr, Changsha 410082, Peoples R China.
关键词：
immunosensor;N-6-(Delta(2)-isopentenyl) adenosine (iPA);bienzyme catalysis;competitive immunoreaction
摘要：
A novel immunosensor based on a multilayer-coated glassy carbon electrode was designed to determine isopentenyl adenosine (iPA) in plants. The multilayer consists of polypyrrole and poly(m-phenylenediamine) with K 4Fe(CN)6 and horseradish peroxidase (HRP) entrapped during electropolymerization. The ferrocyanide doped in polypyrrole functions as the mediator. The glucose oxidase bound on the immunosensor by the competitive immunoreaction involving iPA catalyzed the oxidation of the added glucose with the formation of H2O2, which is in turn reduced in the presence of HRP entrapped in poly(m-phenylenediamine). The current of the oxidized production of ferrocyanide reduced at -50mV is inversely proportional to the concentration of iPA in the competitive immunoreaction. This immunosensor is able to be used about 40 times; after that its surface can be regenerated for a new immunosensor assembly by washing with 0.1M citrate-phosphate buffer (pH 4.6). The percentage of current response reduction (CR%) (y) is linearly related to the logarithm of the concentration of iPA (x) in the 5-300μg/ml range, with a regression equation of the form y=42.13x-27.79 and a correlation coefficient of 0.9861. Five hybrid rice grain samples were analyzed with results in satisfactory agreement to those obtained by high-performance liquid chromatography. © 2003 Elsevier Inc. All rights reserved.
语种：
英文
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A renewable amperometric immunosensor for phytohormone β-indole acetic acid assay
作者：
Li, J;Xiao, LT;Zeng, GM;Huang, GH;Shen, GL* ;...
期刊：
Analytica Chimica Acta ,2003年494(1-2):177-185 ISSN：0003-2670
通讯作者：
Shen, GL
作者机构：
[Shen, GL] Hunan Univ, Chem & Chem Engn Coll, State Key Lab Chemo Biosensing & Chemometr, Dept Environm Sci & Engn, Changsha 410082, Peoples R China.;Hunan Agr Univ, Key Lab Phytohormones Hunan Province, Changsha 410028, Peoples R China.
通讯机构：
[Shen, GL] H;Hunan Univ, Chem & Chem Engn Coll, State Key Lab Chemo Biosensing & Chemometr, Dept Environm Sci & Engn, Changsha 410082, Peoples R China.
关键词：
Amperometric immunosensor;Hapten-protein conjugation;Immunoassay;β-Indole acetic acid
摘要：
A rapid, convenient and accurate method using a sol-gel-alginate-carbon composite electrode (SACE) is described for the determination of β-indole acetic acid (IAA) in plant samples. The determination is based on an enzyme-linked competitive immunoreaction between free IAA and IAA labeled with HRP to bind on the anti-IAA IgG immobilized on the SACE surface. The response signal expressed as percentage current reduction (CR%, y) is linearly related to the logarithm of the concentration of IAA (x) in range of 5-500μgml -1 with a regression equation of the form y=37.80x-22.47 and correlation coefficient of 0.9922. The immunosensor was used to determine the IAA content in the hybrid rice grain samples taken in the growing period. © 2003 Published by Elsevier B.V.
语种：
英文
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Amperometric biosensor with HRP immobilized on a sandwiched nano-Au / polymerized m-phenylenediamine film and ferrocene mediator
作者：
Li, J;Xiao, LT;Liu, XM;Zeng, GM;Huang, GH;...
期刊：
Analytical and Bioanalytical Chemistry ,2003年376(6):902-907 ISSN：1618-2642
通讯作者：
Shen, GL
作者机构：
[Shen, GL] Hunan Univ, Dept Environm Sci & Engn, State Key Lab Chemobiosensing & Chemometr, Chem & Chem Engn Coll, Changsha 410082, Peoples R China.;Hunan Agr Univ, Key Lab Phytohormones Hunan Prov, Changsha 410028, Peoples R China.
通讯机构：
[Shen, GL] H;Hunan Univ, Dept Environm Sci & Engn, State Key Lab Chemobiosensing & Chemometr, Chem & Chem Engn Coll, Changsha 410082, Peoples R China.
关键词：
Enzyme biosensor;H2O2;m-Phenylenediamine polymer;Nano-Au particle
摘要：
An amperometric biosensor has been developed for the determination of H2O2 in plant samples. Horseradish peroxidase (HRP) is immobilized on a sandwiched nano-Au particle / m-phenylenediamine polymer film by glutaraldehyde cross-linking. The film is formulated on the carbon paste electrode (CPE) blended with ferrocene as an electron transfer mediator. On the low concentration range, the current response is related to the H 2O2 concentration linearly from 0 to 8×10 -6 M with a detection limit of 1.3×10-7 M. On a wider concentration range of 8×10-6 to 1.4×10 -4 M, the reciprocal of current response is linearly related to the reciprocal of H2O2 concentration. The apparent Michaelis-Menten constant (Km app) was calculated to be 0.0334 mM. The sensor has been tested by determining H2O2 concentration in plant leaf samples. © Springer-Verlag 2003.
语种：
英文
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A novel piezoelectric biosensor for the detection of phytohormone β-indole acetic acid
作者：
Li, J;Wu, ZY;Xiao, LT;Zeng, GM;Huang, GH;...
期刊：
Analytical Sciences ,2002年18(4):403-407 ISSN：0910-6340
通讯作者：
Shen, GL
作者机构：
[Shen, GL] Hunan Univ, Chem & Chem Engn Coll, Dept Environm Sci & Engn, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Peoples R China.;Hunan Agr Univ, Key Lab Phytohormones, Changsha 410082, Peoples R China.
通讯机构：
[Shen, GL] H;Hunan Univ, Chem & Chem Engn Coll, Dept Environm Sci & Engn, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Peoples R China.
摘要：
A novel piezoelectric immunosensor has been developed for the determination of β-indole acetic acid (IAA) in dilute solutions. The detection is based on competitive immunoreaction between a hapten (IAA) and an antigen (IAA-BSA, hapten-protein conjugation) bound to an anti-IAA antibody, immobilized on a quartz crystal microbalance (QCM). The frequency change (y) of the sensor caused by antigen is linearly related to the logarithm of the concentration of IAA (x) in the range of 0.5 ng/ml - 5 μg/ml with a regression equation of the form y = - 23x + 151 (r = 0.9937).
语种：
英文
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