作者机构：
[Xingyue Zhang; Gang Wan; Ziwei Li; Shuting Xiong; Xiaoqing Wang; Yazhou Hu] College of Fisheries, Hunan Agricultural University, Changsha, China;[Qiufu Wu] Hunan Jiangyu Biotechnology Co., Ltd, Changsha, China;[Lilang You] Nanxun Youyou Family Farm, Huzhou, China
通讯机构：
[Lilang You] N;[Yazhou Hu] C;Nanxun Youyou Family Farm, Huzhou, China<&wdkj&>College of Fisheries, Hunan Agricultural University, Changsha, China
关键词：
P. nigromaculatus;Gonad;Transcriptome;Differentially expressed genes;Sex-related SNPs
摘要：
Pelophylax nigromaculatus is an important aquaculture economic animal in China, with an annual output of more than 80,000 tons in recent years. P. nigromaculatus demonstrates distinct sexual dimorphism, characterized by females being substantially larger than males. Consequently, the cultivation of female populations presents higher economic value. The RNA-Seq technology is harnessed in this study to delve into the sexual differentiation and reproductive mechanisms of these frogs. By analyzing the transcriptomes of mature testes and ovaries, 17.24 Gbp and 23.71 Gbp of clean data were obtained respectively. This analysis yielded 107,897 annotated genes, among which 8099 were differentially expressed; 4370 were upregulated and 3729 were downregulated in ovaries. GO and KEGG analyses highlighted 70 genes exhibiting sexual dimorphism. For validation, 13 differentially expressed genes were subjected to qRT-PCR, corroborating the results of transcriptome sequencing. Furthermore, the transcriptome analysis of P. nigromaculatus’ sexual glands revealed 1,062,663 SNPs, with 676,172 (63.63%) and 386,491 (36.37%) being base conversion and base transversion sites, respectively. Utilizing excel for SNP site analysis, 35,406 SNPs were identified as significantly associated with the sex of P. nigromaculatus. In pursuit of sex-related markers, 100 SNP sites, aligning perfectly with male heterozygosity and female homozygosity, were randomly selected for amplification and sequencing. Through this approach, 134 SNPs were identified, 83 (61.94%) of which matched the transcriptome data. The findings of this study provide an invaluable resource for future functional research on sex-related genes of P. nigromaculatus and offer a foundation for the development of sex-related molecular markers.
通讯机构：
[Zhou, N ] H;Hunan Agr Univ, Hunan Engn Res Ctr Biochar, Changsha 410128, Peoples R China.;Hunan Agr Univ, Coll Chem & Mat Sci, Changsha 410128, Peoples R China.
摘要：
Transition-metal-catalyzed reactive oxygen species (ROS) play an important role in existing bactericidal systems, but there are some problems, such as poor stability, ease of dissolution, and difficulty in reusing. To address the issue of stability, we have developed a sufficient electron-donating (SED) system. In the presence of the two most active transition metals with multiple valences, several as-formed redox pairs can provide sufficient electrons, and then effectively trigger the reduction of H2O/O-2 to produce O-1(2), <middle dot>OH and <middle dot>O-2(-) without any assistance from extra energy or other active oxidants. Consequently, the SED system showed 100% removal efficiency for Escherichia coli in 2 h, and also showed remarkable stability and reusability with 85% inactivation after 11 repetitions. It is worth noting that <middle dot>OH and O-1(2) play a major role in the bactericidal process. While <middle dot>O-2(-) mainly plays a role in promoting the formation of O-1(2) and <middle dot>OH.
作者机构：
[Peng, Liang; Song, Huijuan; Chen, Anwei; Deng, Xiao; Wang, Yuanlong; Kuang, Xiaolin] College of Environment & Ecology, Hunan Agricultural University, Changsha, 410128, PR China;[Song, Huijuan; Chen, Anwei; Deng, Xiao; Wang, Yuanlong; Kuang, Xiaolin] Hunan Engineering and Technology Research Center for Irrigation Water Purification, Changsha, 410128, PR China;[Peng, Liang] Hunan Engineering and Technology Research Center for Irrigation Water Purification, Changsha, 410128, PR China. Electronic address: pengliang2004@126.com;[Ao, Hejun] College of Agronomy, Hunan Agricultural University, Changsha, 410128, PR China
通讯机构：
[Liang Peng] C;College of Environment & Ecology, Hunan Agricultural University, Changsha, 410128, PR China<&wdkj&>Hunan Engineering and Technology Research Center for Irrigation Water Purification, Changsha, 410128, PR China
关键词：
Meteorological factors;Model;Rice;Soil cd;pH
摘要：
During the rice growth cycle, the average available cadmium concentration (C(A-Cd)) in the soil determines the Cd content in rice plant. Given defined soil properties and rice varieties, the meteorological factors play a crucial role in soil's available cadmium concentration (C(Cd)) during the rice growth cycle. Thus, it is significant to investigate the influence of meteorological factors in C(Cd) during the rice growth cycle and develop a predictive model for C(A-Cd). The rice was cultivated under seven different sowing dates in Cd and As-contaminated soil in Hunan Province. Studied the impact of meteorological factors on paddy soil. The results showed that accumulated temperature (AT) and total precipitation (TP) were key factors affecting the soil C(Cd). The correlation coefficients between AT and TP with soil C(A-Cd) were 0.98 and -0.94 (p<0.01), respectively. However, there was no significant correlation with C(As). AT mainly influenced the C(Cd) during the grouting and maturity stages. A straightforward empirical prediction model was developed, capable of accurately forecasting C(A-Cd) during the rice growth cycle by considering meteorological factors and the initial soil C(Cd). This study supported a novel foundation for the precise prediction of Cd content in rice.
摘要：
Effects of steam explosion (SE) treatment (0.3-1.0 MPa, 30-90s) on the physicochemical and functional properties of dietary fiber (DF) extracted from tea residue were investigated. Results showed that SE led to the formation of porous and less compact microstructures of tea residues. Compared with the control sample (12.41 g/100 g), the content of soluble dietary fiber (SDF) was markedly increased by SE, reaching the maximum of 23.03 g/100 g when steam explored at 0.5 MPa for 90 s, due to the degradation of insoluble dietary fiber (IDF). The physicochemical and functional properties were significantly influenced by SE treatments. IDF exhibited decreased water holding capacity (WHC), swelling capacity (SC), oil holding capacity (OHC) after SE, whereas the physicochemical properties of SDF from SE-treated tea residues (0.3 MPa, 90s) were greatly improved. The glucose absorption capacity (GAC), cholesterol adsorption capacity (CAC), and nitrite ion adsorption capacity (NIAC) of both IDF and SDF showed trends of first increasing and then decreasing, indicating that excessive SE treatment resulted in poor properties of DF. These findings are of great value for the high-value utilization of agricultural by-product and development of functional foods.
作者机构：
[Lin, Jiaji; Wu, Haoyang; Wang, Jinyu; Peng, Yi; Lv, Peiyuan] Air Force Med Univ, Affiliated Hosp 2, Dept Neurol, Xian, Peoples R China.;[Lin, Jiaji; Wu, Haoyang; Wang, Jinyu; Lv, Peiyuan] Air Force Med Univ, Basic Med Sch, Xian, Peoples R China.;[Bennett, Brian] NIEHS, Integrat Bioinformat Support Grp, Durham, NC USA.;[Wang, Jiajia] Chinese Acad Sci, Comp Network Informat Ctr, Beijing, Peoples R China.;[Li, Pishun] Hunan Agr Univ, Coll Vet Med, Changsha, Peoples R China.
通讯机构：
[Lin, JJ ] A;[Hu, G ] N;Air Force Med Univ, Affiliated Hosp 2, Dept Neurol, Xian, Peoples R China.;Air Force Med Univ, Basic Med Sch, Xian, Peoples R China.;NIEHS, Epigenet & Stem Cell Biol Lab, Durham, NC 27709 USA.
摘要：
Epigenetic regulators present novel opportunities for both ischemic stroke research and therapeutic interventions. While previous work has implicated that they may provide neuroprotection by potentially influencing coordinated sets of genes and pathways, most of them remain largely uncharacterized in ischemic conditions. In this study, we used the oxygen-glucose deprivation (OGD) model in the immortalized mouse hippocampal neuronal cell line HT-22 and carried out an RNAi screen on epigenetic regulators. PRMT5 was identified as a novel negative regulator of neuronal cell survival after OGD, which presented a phenotype of translocation from the cytosol to the nucleus upon oxygen and energy depletion both in vitro and in vivo. PRMT5 bound to the chromatin and a large number of promoter regions to repress downstream gene expression. Silencing Prmt5 significantly dampened the OGD-induced changes for a large-scale of genes, and gene ontology analysis showed that PRMT5-target genes were highly enriched for Hedgehog signaling. Encouraged by the above observation, mice were treated with middle cerebral artery occlusion with the PRMT5 inhibitor EPZ015666 and found that PRMT5 inhibition sustains protection against neuronal death in vivo. Together, these findings revealed a novel epigenetic mechanism of PRMT5 in cerebral ischemia and uncovered a potential target for neuroprotection.
摘要：
In this study, the speciation, leachability, phytoaccessibility, and environmental risks of heavy metals (Cd, Zn, and Cu) during liquefaction of contaminated peanut straw in ethanol at different temperatures (220, 260, 300, 340, and 380 degrees C) were comprehensively investigated. The results showed that elevated temperatures facilitated heavy metal accumulation in the biochar. The acid-soluble/exchangeable and reducible fraction percentages of heavy metals were substantially reduced in the biochar after liquefaction as the temperature increased, and the oxidizable fraction became the dominant heavy metal fraction, accounting for 44.14-78.67%. Furthermore, although an excessively high liquefaction temperature (380 degrees C) increased the residual fraction percentages of Zn and Cu, it was detrimental to Cd immobilization. The acid-soluble/exchangeable Cd in the contaminated peanut straw readily migrates to the bio-oil during liquefaction, with the highest concentration of 1.60 mg/kg at 260 degrees C liquefaction temperature, whereas Zn and Cu are predominantly bound to the unexchangeable fraction in the bio-oil. Liquefaction inhibited heavy metal leachability and phytoaccessibility in biochar, the lowest extraction rates of Cd, Zn, and Cu were 0.71%, 1.66% and 0.95% by diethylenetriamine pentaacetic acid, respectively. However, the leaching and extraction concentrations increased when the temperature was raised to 380 degrees C. Additionally, heavy metal risk was reduced from medium and high risk to no and low risk. In summary, liquefaction reduces heavy metal toxicity and the risks associated with contaminated peanut straw, and a temperature range of 300-340 degrees C for ethanol liquefaction can be considered optimal for stabilizing heavy metals.
摘要：
This study provided a new perspective on regulating strawberry flavor during storage by maintaining fungal community balance with active packaging film. Compared with initial strawberries (IS), gelatin composite film-packaged strawberries (CG) showed pleasant fruity and fragrance flavor whose relative content of most esters (ethyl acetate, butyl acetate, etc), 2-hexen-1-ol, hexanal D, and 3-pentanone increased, while other treated strawberries possessed alcoholic, sour, or moldy flavor. Furthermore, the relative abundance of fungal community (Mycosphaerella, Botrytis, Papiliotrema, Vishniacozyma, Filobasidium, etc) in CG was similar to that of IS. However, Botrytis was the dominant fungal genus in other treated strawberries and their fungal diversity decreased. Spearman correlation coefficient indicated that Papiliotrema, Vishniacozyma, and Filobasidium were highly correlated with flavor formation of strawberries (esters, alcohols, aldehydes, etc). Therefore, this work contributed to understanding the effect of fungal community on flavor formation of strawberry fruit during storage and also revealing the great potential of gelatin composite film on maintaining the quality of postharvest strawberries during storage.
期刊：
Postharvest Biology and Technology,2024年212:112890 ISSN：0925-5214
通讯作者：
Shang Guo
作者机构：
[Hongrui Lv; Zhenzhen Wu; Xiaojie Nan; Min Zhu; Kai Mao] Shanxi Institute for Functional Food, Shanxi Agricultural University, Taiyuan 030031, China;College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China;[Shang Guo] Shanxi Institute for Functional Food, Shanxi Agricultural University, Taiyuan 030031, China<&wdkj&>College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China
通讯机构：
[Shang Guo] S;Shanxi Institute for Functional Food, Shanxi Agricultural University, Taiyuan 030031, China<&wdkj&>College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China
摘要：
Daylily flower buds are a good source of nutrients and contain lots of bioactive compounds, but they are subject to deterioration after harvest, which limits the taste and consumer satisfaction. Hydrogen sulfide (H2S) has been regarded as an important gaseous signaling molecule, which could prolong postharvest freshness of fruits and vegetables. The present study aims to investigate the effects of H2S on postharvest quality and metabolism changes of daylily flower buds during storage. The results showed that 3.2 mM NaHS (H2S donor) effectively inhibited weight loss, blooming and decay of daylily during storage. H2S application markedly reduced the accumulation of malondialdehyde and H2O2. H2S treatment also delayed the loss of total protein and antioxidant glutathione, and maintained the activities of antioxidant enzymes superoxide dismutase and catalase at higher level compared to control group. Widely targeted metabolomics identified various differentially accumulated metabolites (DAMs) during storage and with H2S treatment of daylily. These DAMs largely distributed in the classes of amino acids, organic acids, sugars and alcohols, terpenoids. A special focus was given to 303 common DAMs between the comparison of CK0 vs. CK6 and CK6 vs. T6, of which the DAMs were mainly enriched in the KEGG pathways of monobactam biosynthesis, pyrimidine metabolism, glycine, serine and threonine metabolism, arginine biosynthesis, pentose and glucuronate interconversions. Four DAMs including Cytidine 5′-Monophosphate, Fumaric Acid, Hydroxypyruvic Acid, 2′-Deoxycytidine-5′-Monophosphoric Acid exhibited great downregulation during storage, and H2S treatment dramatically recovered their relative contents. This study indicated that H2S treatment offers the potential to preserve the quality of daylily flower buds and extend their postharvest life.
