Effects of Pu-erh ripened tea on hyperuricemic mice studied by serum metabolomics
作者：
Zhao, Ran;Chen, Dong* ;Wu, Hualing*
期刊：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES ,2017年1068-1069:149-156 ISSN：1570-0232
通讯作者：
Chen, Dong;Wu, Hualing
作者机构：
[Zhao, Ran; Chen, Dong; Chen, D; Wu, HL; Wu, Hualing] Guangdong Acad Agr Sci, Tea Res Inst, Guangzhou 510640, Guangdong, Peoples R China.;[Zhao, Ran] Hunan Agr Univ, Key Lab, Minist Educ Tea Sci, Changsha 410128, Hunan, Peoples R China.;[Zhao, Ran; Chen, Dong; Wu, Hualing] Guangdong Key Lab Tea Plant Resources Innovat & U, Guangzhou 510640, Guangdong, Peoples R China.
通讯机构：
[Chen, D; Wu, HL] G;Guangdong Acad Agr Sci, Tea Res Inst, Guangzhou 510640, Guangdong, Peoples R China.
关键词：
GC-MS;Hyperuricemia;Metabolomics;Pu-erh ripened tea
摘要：
To evaluate effects of Pu-erh ripened tea in hyperuricemic mice, a mouse hyperuricemia model was developed by oral administration of potassium oxonate for 7 d. Serum metabolomics, based on gas chromatography–mass spectrometry, was used to generate metabolic profiles from normal control, hyperuricemic and allopurinol-treated hyperuricemic mice, as well as hyperuricemic mice given Pu-erh ripened tea at three doses. Pu-erh ripened tea significantly lowered serum uric acid levels. Twelve potential biomarkers associated with hyperuricemia were identified. Pu-erh ripened tea and allopurinol differed in their metabolic effects in the hyperuricemic mice. Levels of glutamic acid, indolelactate, L-allothreonine, nicotinoylglycine, isoleucine, L-cysteine and glycocyamine, all involved in amino acid metabolism, were significantly changed in hyperuricemic mice treated Pu-erh ripened tea. Thus, modulating amino acid metabolism might be the primary mechanism of anti-hyperuricemia by Pu-erh ripened tea. ©2017 Elsevier B.V.
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ANGPTL3 is part of the machinery causing dyslipidemia majorily via LPL inhibition in mastitis mice
作者：
Xiao, Hong-Bo* ;Wang, Ji-Ying;Sun, Zhi-Liang
期刊：
Experimental and Molecular Pathology ,2017年103(3):242-248 ISSN：0014-4800
通讯作者：
Xiao, Hong-Bo
作者机构：
[Xiao, Hong-Bo; Wang, Ji-Ying] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Sun, Zhi-Liang] Hunan Agr Univ, Biol Vet Drugs Branch, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Hunan, Peoples R China.;[Wang, Ji-Ying] Huai Hua Vocat & Tech Coll, Dept Anim Sci & Technol, Huaihua 418000, Peoples R China.
通讯机构：
[Xiao, Hong-Bo] H;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
关键词：
*Angiopoietin-like protein 3;*Hyperlipidemia;*Lipoprotein lipase;*Mastitis;*Mice
摘要：
Previous investigations have shown that inflammation induces changes in lipid and lipoprotein metabolism, and increased expression of angiopoietin-like protein 3 (ANGPTL3) contributes to the development of dyslipidemia. Here we investigated whether there is a correlation between increased ANGPTL3 expression and dyslipidemia in mastitis mice. Thirty mice were divided into two groups: control group and Staphylococcus aureus (S. aureus)–induced mastitis mice group. Changes in the levels of blood lipids [total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C)]; activity of myeloperoxidase (MPO); concentrations of plasma inflammation biomarkers [interferon-γ (IFNγ), tumor necrosis factor α (TNFα), and interleukin-1α (IL-1α)]; concentration of plasma ANGPTL3 protein; lipoprotein lipase (LPL) activities in postheparin plasma; expressions of hepatic N-acetylgalactosaminyltransferase 2 (GALNT2), hepatic ANGPTL3 and adipose LPL were determined. The major results indicated specific pathological mammary tissue changes, elevated MPO activity, reduced GALNT2 mRNA expression, elevated ANGPTL3 mRNA and protein expression and reduced LPL mRNA and protein expression. In plasma samples the S.aureus infused mice displayed elevated ANGPTL3 protein concentration, TG, TC and LDL-C levels, and reduced postheparin LPL activities and HDL-C level. The data suggests that ANGPTL3 is part of the machinery causing dyslipidemia majorily via LPL inhibition in mastitis mice. © 2017 Elsevier Inc.
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英文
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Tea polyphenol epigallocatechin gallate inhibits Escherichia coli by increasing endogenous oxidative stress
作者：
Xiong, Li-Gui;Chen, Yi-Jun;Tong, Jie-Wen;Huang, Jian-An;Li, Juan;...
期刊：
Food Chemistry ,2017年217:196-204 ISSN：0308-8146
通讯作者：
Gong, Yu-Shun;Liu, Zhong-Hua
作者机构：
[Gong, Yu-Shun; Huang, Jian-An; Liu, Zhong-Hua; Xiong, Li-Gui; Chen, Yi-Jun; Li, Juan; Tong, Jie-Wen] Hunan Agr Univ, Minist Educ, Key Lab Tea Sci, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhong-Hua; Xiong, Li-Gui] Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Funct Ingredie, Changsha 410128, Hunan, Peoples R China.;[Huang, Jian-An; Liu, Zhong-Hua; Xiong, Li-Gui; Li, Juan] Hunan Agr Univ, Collaborat Innovat Ctr Utilisat Funct Ingredients, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Gong, Yu-Shun; Liu, Zhong-Hua] H;Hunan Agr Univ, Minist Educ, Key Lab Tea Sci, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Funct Ingredie, Changsha 410128, Hunan, Peoples R China.
关键词：
Adaptive response;Antibacterial;Epigallocatechin gallate;Escherichia coli;Reactive oxygen species
摘要：
The antibacterial effects of tea polyphenol epigallocatechin gallate (EGCG), a common phytochemical with a number of potential health benefits, are well known. However, the mechanism of its bactericidal action remains unclear. Using E. coli as a model organism, it is argued here that H2O2 synthesis by EGCG is not attributed to its inhibitory effects. In contrast, the bactericidal action of EGCG was a result of increased intracellular reactive oxygen species and blunted adaptive oxidative stress response in E. coli due to the co-administration of antioxidant N-acetylcysteine, and not on account of exogenous catalase. Furthermore, we noted a synergistic bactericidal effect for EGCG when combined with paraquat. However, under anaerobic conditions, the inhibitory effect of EGCG was prevented. In conclusion, EGCG caused an increase in endogenous oxidative stress in E. coli, thereby inhibiting its growth, and hence the use of EGCG as a prooxidant is supported by this study. © 2016 Elsevier Ltd
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英文
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L-Theanine Improves Immunity by Altering TH2/TH1 Cytokine Balance, Brain Neurotransmitters, and Expression of Phospholipase C in Rat Hearts
作者：
Li, Chengjian;Tong, Haiou;Yan, Qiongxian* ;Tang, Shaoxun;Han, Xuefeng;...
期刊：
Medical Science Monitor ,2016年22:662-669 ISSN：1643-3750
通讯作者：
Xiao, Wenjun;Yan, Qiongxian
作者机构：
[Tan, Zhiliang; Han, Xuefeng; Xiao, Wenjun; Tong, Haiou; Li, Chengjian] Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Bot Funct Ingr, Prov Coinnovat Ctr Utilizat Bot Funct Ingredients, Changsha, Hunan, Peoples R China.;[Tang, Shaoxun; Yan, Qiongxian; Tan, Zhiliang; Han, Xuefeng; Tong, Haiou; Li, Chengjian] Chinese Acad Sci, Key Lab Agroecol Proc Subtrop Reg, Hunan Res Ctr Livestock & Poultry Sci,Inst Subtro, South Cent Expt Stn Anim Nutr & Feed Sci,Minist A, Changsha, Hunan, Peoples R China.;[Li, Chengjian] Yongzhou Vocat Tech Coll, Dept Pharm, Yongzhou, Hunan, Peoples R China.
通讯机构：
[Xiao, Wenjun] H;[Yan, Qiongxian] C;Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Bot Funct Ingr, Prov Coinnovat Ctr Utilizat Bot Funct Ingredients, Changsha, Hunan, Peoples R China.;Chinese Acad Sci, Key Lab Agroecol Proc Subtrop Reg, Hunan Res Ctr Livestock & Poultry Sci,Inst Subtro, South Cent Expt Stn Anim Nutr & Feed Sci,Minist A, Changsha, Hunan, Peoples R China.
关键词：
Immunity;L-theanine;Neurotransmitter agents;Phosphoinositide phospholipase c;Th1-Th2 Balance
摘要：
Background: This study aimed to investigate the regulatory effects of L-theanine on secretion of immune cytokines, hormones, and neurotransmitters, and mRNA expression of phospholipase C (PLC) in rats, and to explore its regulatory mechanism in immune function. Material/Methods: Sixty-four Sprague-Dawley rats received daily intragastric infusion of different doses of L-theanine solution [0, 50 (LT), 200 (MT), and 400 (HT) mg/kg BW]. Cytokines, immunoglobulins, and hormones in the serum, neurotransmitters, and mRNA expression of PLC in the relevant tissues were assayed. Results: L-theanine administration increased the splenic organ index and decreased the contents of ILs-4/6/10 and the ratio of IL-4/IFN-g in the serum. High-dose L-theanine administration increased the levels of dopamine and 5-hydroxytryptamine in the pituitary and hippocampus, resulting in decrease in corticosterone level in the serum. L-theanine administration decreased the mRNA expressions of PLC isomers in the liver and PLC-g1 and PLC-d1 in the spleen. Interestingly, mRNA expressions of PLC-b1 in the spleen and PLC isomers mRNA in the heart were up-regulated by L-theanine administration. Conclusions: Administration of 400 mg/kg BWL-theanine improved immune function of the rats by increasing the splenic weight, altering the Th2/Th1 cytokine balance, decreasing the corticosterone level in the serum, elevating dopamine and 5-hydroxytryptamine in the brain, and regulating the mRNA expression of PLC isomers in the heart. © Med Sci Monit, 2016.
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英文
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Convergence and divergence of bitterness biosynthesis and regulation in Cucurbitaceae
作者：
Zhou, Yuan;Ma, Yongshuo;Zeng, Jianguo;Duan, Lixin;Xue, Xiaofeng;...
期刊：
NATURE PLANTS ,2016年2(12):16183 ISSN：2055-026X
通讯作者：
Shang, Yi;Huang, Sanwen
作者机构：
[Zhang, Huimin; Lin, Tao; Huang, Sanwen; Shang, Yi; Huang, SW; Liu, Xinyan; Zhang, Zhonghua; Wang, Huaisong; Zhong, Yang; Liu, Min; Zhou, Yuan; Ma, Yongshuo; Zhang, Shu] Chinese Acad Agr Sci, Inst Vegetables & Flowers, Key Lab Biol & Genet Improvement Hort Crops, Minist Agr,Sinodutch Joint Lab Hort Genom, Beijing 100081, Peoples R China.;[Lin, Tao; Huang, Sanwen; Shang, Yi; Huang, SW; Zhou, Yuan] Chinese Acad Agr Sci, Agr Genome Inst Shenzhen, Shenzhen 518124, Peoples R China.;[Liu, Xiubin; Zeng, Jianguo; Zhou, Yuan; Huang, Peng] Hunan Agr Univ, Hort & Landscape Coll, Natl Chinese Med Herbs Hunan Technol Ctr, Changsha 410000, Hunan, Peoples R China.;[Duan, Lixin] Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China.;[Xue, Xiaofeng] Chinese Acad Agr Sci, Inst Apiculture Res, Beijing 100093, Peoples R China.
通讯机构：
[Shang, Y; Huang, SW] C;Chinese Acad Agr Sci, Inst Vegetables & Flowers, Key Lab Biol & Genet Improvement Hort Crops, Minist Agr,Sinodutch Joint Lab Hort Genom, Beijing 100081, Peoples R China.;Chinese Acad Agr Sci, Agr Genome Inst Shenzhen, Shenzhen 518124, Peoples R China.
摘要：
Differentiation of secondary metabolite profiles in closely related plant species provides clues for unravelling biosynthetic pathways and regulatory circuits, an area that is still underinvestigated. Cucurbitacins, a group of bitter and highly oxygenated tetracyclic triterpenes, are mainly produced by the plant family Cucurbitaceae. These compounds have similar structures, but differ in their antitumour activities and ecophysiological roles. By comparative analyses of the genomes of cucumber, melon and watermelon, we uncovered conserved syntenic loci encoding metabolic genes for distinct cucurbitacins. Characterization of the cytochrome P450s (CYPs) identified from these loci enabled us to unveil a novel multi-oxidation CYP for the tailoring of the cucurbitacin core skeleton as well as two other CYPs responsible for the key structural variations among cucurbitacins C, B and E. We also discovered a syntenic gene cluster of transcription factors that regulates the tissue-specific biosynthesis of cucurbitacins and may confer the loss of bitterness phenotypes associated with convergent domestication of wild cucurbits. This study illustrates the potential to exploit comparative genomics to identify enzymes and transcription factors that control the biosynthesis of structurally related yet unique natural products. © 2016 Macmillan Publishers Limited, part of Springer Nature. All rights reserved.
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英文
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Dual and Opposing Roles of Xanthine Dehydrogenase in Defense-Associated Reactive Oxygen Species Metabolism in Arabidopsis
作者：
Ma, Xianfeng;Wang, Wenming;Bittner, Florian;Schmidt, Nadine;Berkey, Robert;...
期刊：
PLANT CELL ,2016年28(5):1108-1126 ISSN：1040-4651
通讯作者：
Xiao, Shunyuan;Xiong, Xingyao
作者机构：
[Ma, Xianfeng; Tan, Liqiang; Zhang, Yi; Feng, Jiayue; Berkey, Robert; Xiao, Shunyuan; Zhang, Qiong; Wen, Yinqiang; King, Harlan] Univ Maryland, Inst Biosci & Biotechnol Res, College Pk, MD 20850 USA.;[Ma, Xianfeng; Tan, Liqiang; Zhang, Yi; Feng, Jiayue; Berkey, Robert; Xiao, Shunyuan; Zhang, Qiong; Wen, Yinqiang; King, Harlan] Univ Maryland, Dept Plant Sci & Landscape Architecture, College Pk, MD 20850 USA.;[Ma, Xianfeng; Deng, Ziniu; Xiong, Xingyao] Hunan Agr Univ, Hunan Prov Key Lab Germplasm Innovat & Utilizat C, Changsha 410128, Hunan, Peoples R China.;[Ma, Xianfeng; Zhang, Lingli; Wang, Wenming] Sichuan Agr Univ, Rice Res Inst, Chengdu 611130, Peoples R China.;[Schmidt, Nadine; Bittner, Florian] Braunschweig Univ Technol, Dept Plant Biol, D-38106 Braunschweig, Germany.
通讯机构：
[Xiao, Shunyuan] U;[Xiong, Xingyao] H;[Xiong, Xingyao] C;Univ Maryland, Inst Biosci & Biotechnol Res, College Pk, MD 20850 USA.;Univ Maryland, Dept Plant Sci & Landscape Architecture, College Pk, MD 20850 USA.
摘要：
While plants produce reactive oxygen species (ROS) for stress signaling and pathogen defense, they need to remove excessive ROS induced during stress responses in order to minimize oxidative damage. How can plants fine-tune this balance and meet such conflicting needs? Here, we show that XANTHINE DEHYDROGENASE1 (XDH1) in Arabidopsis thaliana appears to play spatially opposite roles to serve this purpose. Through a large-scale genetic screen, we identified three missense mutations in XDH1 that impair XDH1’s enzymatic functions and consequently affect the powdery mildew resistance mediated by RESISTANCE TO POWDERY MILDEW8 (RPW8) in epidermal cells and formation of xanthine-enriched autofluorescent objects in mesophyll cells. Further analyses revealed that in leaf epidermal cells, XDH1 likely functions as an oxidase, along with the NADPH oxidases RbohD and RbohF, to generate superoxide, which is dismutated into H2O2. The resulting enrichment of H2O2 in the fungal haustorial complex within infected epidermal cells helps to constrain the haustorium, thereby contributing to RPW8-dependent and RPW8-independent powdery mildew resistance. By contrast, in leaf mesophyll cells, XDH1 carries out xanthine dehydrogenase activity to produce uric acid in local and systemic tissues to scavenge H2O2 from stressed chloroplasts, thereby protecting plants from stress-induced oxidative damage. Thus, XDH1 plays spatially specified dual and opposing roles in modulation of ROS metabolism during defense responses in Arabidopsis. © 2016 American Society of Plant Biologists. All rights reserved.
语种：
英文
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Calcium/calmodulin alleviates substrate inhibition in a strawberry UDP-glucosyltransferase involved in fruit anthocyanin biosynthesis
作者：
Peng, Hui;Yang, Tianbao* ;Whitaker, Bruce D.;Shangguan, Lingfei;Fang, Jinggui
期刊：
BMC Plant Biology ,2016年16(1):1-11 ISSN：1471-2229
通讯作者：
Yang, Tianbao
作者机构：
[Yang, Tianbao; Whitaker, Bruce D.; Shangguan, Lingfei; Peng, Hui] USDA ARS, Beltsville Agr Res Ctr, Food Qual Lab, Beltsville, MD 20705 USA.;[Peng, Hui] Hunan Agr Univ, Hort & Landscape Coll, Changsha 410128, Hunan, Peoples R China.;[Shangguan, Lingfei; Fang, Jinggui] Nanjing Agr Univ, Coll Hort, Nanjing 210095, Jiangsu, Peoples R China.
通讯机构：
[Yang, Tianbao] U;USDA ARS, Beltsville Agr Res Ctr, Food Qual Lab, Beltsville, MD 20705 USA.
关键词：
Fragaria vesca;Pelargonidin;Calcium signaling;UGT;Enzyme kinetics
摘要：
Background: UDP-glucosyltransferase (UGT) is a key enzyme for anthocyanin biosynthesis, which by catalyzing glycosylation of anthocyanidins increases their solubility and accumulation in plants. Previously we showed that pre-harvest spray of CaCl2 enhanced anthocyanin accumulation in strawberry fruit by stimulating the expression of anthocyanin structural genes including a fruit specific FvUGT1. Results: To further understand the regulation of anthocyanin biosynthesis, we conducted kinetic analysis of recombinant FvUGT1 on glycosylation of pelargonidin, the major anthocyanidin in strawberry fruit. At the fixed pelargonidin concentration, FvUGT1 catalyzed the sugar transfer from UDP-glucose basically following Michaelis-Menten kinetics. By contrast, at the fixed UDP-glucose concentration, pelargonidin over 150 μM exhibited marked partial substrate inhibition in an uncompetitive mode. These results suggest that the sugar acceptor at high concentration inhibits FvUGT1 activity by binding to another site in addition to the catalytic site. Furthermore, calcium/calmodulin specifically bound FvUGT1 at a site partially overlapping with the interdomain linker, and significantly relieved the substrate inhibition. In the presence of 0.1 and 0.5 μM calmodulin, V max was increased by 71.4 and 327 %, respectively. Conclusions: FvUGT1 activity is inhibited by anthocyanidin, the sugar acceptor substrate, and calcium/calmodulin binding to FvUGT1 enhances anthocyanin accumulation via alleviation of this substrate inhibition. © 2016 The Author(s).
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英文
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Separation of catechins and O-methylated (-)-epigallocatechin gallate using polyamide thin-layer chromatography
作者：
Wang, Kunbo;Chen, Qincao;Lin, Yong* ;Yu, Shuangshang;Lin, Haiyan;...
期刊：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES ,2016年1017-1018:221-225 ISSN：1570-0232
通讯作者：
Lin, Yong;Liu, Zhonghua
作者机构：
[Liu, Zhonghua; Lin, Haiyan; Wang, Kunbo; Lin, Yong; Huang, Jianan] Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhonghua; Lin, Haiyan; Wang, Kunbo; Lin, Yong; Huang, Jianan] Hunan Agr Univ, Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha 410128, Hunan, Peoples R China.;[Lin, Y; Liu, Zhonghua; Yu, Shuangshang; Lin, Haiyan; Wang, Kunbo; Lin, Yong; Huang, Jianan; Chen, Qincao] Hunan Agr Univ, Minist Educ, Key Lab Tea Sci, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Lin, Y; Liu, ZH] H;Hunan Agr Univ, Minist Educ, Key Lab Tea Sci, Changsha 410128, Hunan, Peoples R China.
关键词：
Catechins;Double-development;O-methylated (-)-epigallocatechin gallate;Tea;Thin-layer chromatography
摘要：
Thin-layer chromatography (TLC) method for the separation and quantitative determination of seven related compounds: (+)-catechin (C), (-)-epicatechin (EC), (-)-epigallocatechin (EGC), (-)-epicatechin gallate (ECG), (-)-epigallocatechin gallate (EGCG), (-)-epigallocatechin-3-O-(3-O-methyl) gallate (EGCG3 '' Me) and (-)-epigallocatechin- 3-O-(4-O-methyl) gallate (EGCG4 '' Me) has been developed. The above-mentioned seven compounds have been resolved using polyamide TLC plates using a double-development with methanol followed by acetone/acetic acid (2:1, v/v). In addition, separation of the phenolic acids namely gallic acid, chlorogenic acid, and caffeic acid was achieved using the same solvent system. The applicability of the method was checked by screening of extracts of green, black, oolong, white tea and tea cultivars leaves. (C) 2016 Elsevier B.V. All rights reserved.
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英文
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Comparative transcriptome analysis reveals a global insight into molecular processes regulating citrate accumulation in sweet orange (Citrus sinensis)
作者：
Lu, Xiaopeng;Cao, Xiongjun;Li, Feifei;Li, Jing;Xiong, Jiang;...
期刊：
Physiologia Plantarum ,2016年158(4):463-482 ISSN：0031-9317
通讯作者：
Xie, Shenxi
作者机构：
[Xie, Shenxi; Lu, Xiaopeng; Long, Guiyou; Xiong, Jiang; Li, Jing; Cao, Xiongjun; Li, Feifei] Hunan Agr Univ, Dept Hort, Coll Hort & Landscape, Changsha, Hunan, Peoples R China.;[Xie, Shenxi; Lu, Xiaopeng; Long, Guiyou; Xiong, Jiang; Li, Jing; Cao, Xiongjun] Natl Ctr Citrus Improvement, Changsha, Hunan, Peoples R China.;[Li, Feifei] Hunan Acad Agr Sci, Inst Hort, Changsha, Hunan, Peoples R China.;[Cao, Shangyin] Chinese Acad Agr Sci, Zhengzhou Fruit Res Inst, Zhengzhou, Peoples R China.
通讯机构：
[Xie, Shenxi] H;[Xie, Shenxi] N;Hunan Agr Univ, Dept Hort, Coll Hort & Landscape, Changsha, Hunan, Peoples R China.;Natl Ctr Citrus Improvement, Changsha, Hunan, Peoples R China.
摘要：
Citrate, the predominant organic acid in citrus, determines the taste of these fruits. However, little is known about the synergic molecular processes regulating citrate accumulation. Using ‘Dahongtiancheng’ (Citrus sinensis) and ‘Bingtangcheng’ (C. sinensis) with significant difference in citrate, the objectives of this study were to understand the global mechanisms of high-citrate accumulation in sweet orange. ‘Dahongtiancheng’ and ‘Bingtangcheng’ exhibit significantly different patterns in citrate accumulation throughout fruit development, with the largest differences observed at 50–70 days after full bloom (DAFB). Comparative transcriptome profiling was performed for the endocarps of both cultivars at 50 and 70 DAFB. Over 34.5 million clean reads per library were successfully mapped to the reference database and 670–2630 differentially expressed genes (DEGs) were found in four libraries. Among the genes, five transcription factors were ascertained to be the candidates regulating citrate accumulation. Functional assignments of the DEGs indicated that photosynthesis, the citrate cycle and amino acid metabolism were significantly altered in ‘Dahongtiancheng’. Physiological and molecular analyses suggested that high photosynthetic efficiency and partial impairment of citrate catabolism were crucial for the high-citrate trait, and amino acid biosynthesis was one of the important directions for citrate flux. The results reveal a global insight into the gene expression changes in a high-citrate compared with a low-citrate sweet orange. High accumulating efficiency and impaired degradation of citrate may be associated with the high-citrate trait of ‘Dahongtiancheng’. Findings in this study increase understanding of the molecular processes regulating citrate accumulation in sweet orange. © 2016 Scandinavian Plant Physiology Society
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英文
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The antioxidant effect ofAsparagus cochinchinensis(Lour.) Merr. shoot in d-galactose induced mice aging model andin vitro
作者：
Lei, Linghua;Ou, Lijun;Yu, Xiaoying*
期刊：
Journal of the Chinese Medical Association ,2016年79(4):205-211 ISSN：1726-4901
通讯作者：
Yu, Xiaoying
作者机构：
[Lei, Linghua; Yu, Xiaoying] Hunan Agr Univ, Coll Hort & Landscape, Dept Ornamental Hort, Block North,11th Teaching Bldg,1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Lei, Linghua] Huaihua Univ, Dept Life Sci, Huaihua, Hunan, Peoples R China.;[Ou, Lijun] Agr Sci Acad Hunan Prov, Vegetable Res Inst, Changsha, Hunan, Peoples R China.
通讯机构：
[Yu, Xiaoying] H;Hunan Agr Univ, Coll Hort & Landscape, Dept Ornamental Hort, Block North,11th Teaching Bldg,1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.
关键词：
antioxidant;Asparagus cochinchinensis (Lour.) Merr.;enzyme activity;gene expression;shoot
摘要：
Background: An increasing number of plant components and their extracts have been shown to have beneficial health effects in humans. We aimed to explore the antioxidant effects of the aqueous extract of Asparagus cochinchinensis (Lour.) Men. shoot in vivo and in vitro. Methods: A total of 80 Kun Ming mice were randomly divided into four groups (20/group). The mice in the control group received a daily subcutaneous injection of saline. A daily injection of D-galactose was administered to the aging model group, the vitamin C (Vc) group (positive control group), and the extract treatment group. Regular measurement of blood cells, nitric oxide synthase (NOS), catalase (CAT) activities, superoxide dismutase (SOD) activities, nitric oxide (NO), and malondialdehyde (MDA) concentration, and the expressions of NOS, SOD, and glutathione peroxidase (GPX) in serum levels were obtained. Furthermore, the microstructure of mice viscera was observed using hematoxylin and eosin staining. Results: The aqueous extract of A. cochinchinensis (Lour.) Men. had similar 1,1-diphenyl-2-picrylhydrazyl radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH center dot) [or 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS)] and higher hydroxyl radicals (or superoxide anion; p < 0.05) radical scavenging capabilities to Vc. Moreover, compared with the aging model group, the aqueous extract of A. cochinchinensis (Lour.) Men. shoot could obviously increase NOS, CAT, and SOD activities and the NO content, and reduce the MDA content (p < 0.05). Additionally, the microstructure of mice viscera was obviously improved and the expressions of NOS, SOD and GPX were also manifestly increased in the treatment group (p < 0.05). Conclusion: The aqueous extract of A. cochinchinensis (Lour.) Men. shoot had a strong radical scavenging capability in vivo and in vitro, and might be used to diminish radicals in the body and consequently prevent aging. Copyright (C) 2016, the Chinese Medical Association. Published by Elsevier Taiwan LLC.
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英文
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Metal-Free Photoredox Catalyzed Cyclization of O-(2,4-Dinitrophenyl)oximes to Phenanthridines
作者：
Liu, Xiubin;Qing, Zhixing;Cheng, Pi* ;Zheng, Xinyu;Zeng, Jianguo;...
期刊：
Molecules ,2016年21(12) ISSN：1420-3049
通讯作者：
Cheng, Pi;Xie, Hongqi
作者机构：
[Liu, Xiubin; Qing, Zhixing; Xie, Hongqi; Zheng, Xinyu; Zeng, Jianguo; Cheng, P; Xie, HQ; Cheng, Pi] Hunan Agr Univ, Natl & Prov Union Engn Res Ctr Vet Herbal Med Res, Changsha 410128, Hunan, Peoples R China.;[Liu, Xiubin; Qing, Zhixing; Xie, Hongqi; Zeng, Jianguo; Cheng, P; Xie, HQ; Cheng, Pi] Hunan Agr Univ, Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Cheng, P; Xie, HQ] H;Hunan Agr Univ, Natl & Prov Union Engn Res Ctr Vet Herbal Med Res, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha 410128, Hunan, Peoples R China.
关键词：
visible light;photoredox catalysis;eosin Y;O-aryl oximes;phenanthridines
摘要：
A metal-free visible-light photoredox-catalyzed intermolecular cyclization reaction of O-2,4-dinitrophenyl oximes to phenanthridines was developed. In this study, the organic dye eosin Y and i-Pr2NEt were used as photocatalyst and terminal reductant, respectively. The oxime substrates were transformed into iminyl radical intermediates by single-electron reduction, which then underwent intermolecular homolytic aromatic substitution (HAS) reactions to give phenanthridine derivatives.
语种：
英文
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酵母产物对断奶仔猪肠道形态、屏障功能、细胞因子表达和抗氧化系统的影响
作者：
Yang, Huan-sheng;Wu, Fei;Long, Li-na;Li, Tie-jun;Xiong, Xia* ;...
期刊：
浙江大学学报(英文版)B辑：生物医学与生物技术 ,2016年17(10):752-762 ISSN：1673-1581
通讯作者：
Xiong, Xia;Liu, Hong-nan
作者机构：
[Yang, Huan-sheng; Xiong, Xia; Liu, Hong-nan; Wu, Fei; Long, Li-na; Yin, Yu-long; Li, Tie-jun; Liao, Peng] Chinese Acad Sci, Key Lab Agroecol Proc Subtrop Reg,Minist Agr,Inst, Hunan Prov Engn Res Ctr Hlth Livestock & Poultry, Observat & Expt Stn Anim Nutr & Feed Sci South Ch, Changsha 410125, Hunan, Peoples R China.;[Yang, Huan-sheng] Hunan Normal Univ, Sch Life Sci, Changsha 410006, Hunan, Peoples R China.;[Yang, Huan-sheng] Fujian Aonong Biotechnol Co Ltd, Xiamen 361008, Peoples R China.;[Xiong, Xia] Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Bot Funct Ingr, Prov Coinnovat Ctr Utilizat Bot Funct Ingredients, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Xiong, X; Liu, HN] C;[Xiong, Xia] H;Chinese Acad Sci, Key Lab Agroecol Proc Subtrop Reg,Minist Agr,Inst, Hunan Prov Engn Res Ctr Hlth Livestock & Poultry, Observat & Expt Stn Anim Nutr & Feed Sci South Ch, Changsha 410125, Hunan, Peoples R China.;Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Bot Funct Ingr, Prov Coinnovat Ctr Utilizat Bot Funct Ingredients, Changsha 410128, Hunan, Peoples R China.
关键词：
Yeast products;Weaned piglet;Intestinal morphology;Barrier function;Antioxidant system
摘要：
The goal of this study was to evaluate the effects of a mixture of yeast culture, cell wall hydrolysates, and yeast extracts (collectively “yeast products,” YP) on the performance, intestinal physiology, and health of weaned piglets. A total of 90 piglets weaned at 21 d of age were blocked by body weight, sex, and litter and randomly assigned to one of three treatments for a 14-d feeding experiment, including (1) a basal diet (control), (2) 1.2 g/kg of YP, and (3) 20 mg/kg of colistin sulfate (CSE). No statistically significant differences were observed in average daily feed intake, average daily weight gain, or gain-to-feed ratio among CSE, YP, and control piglets. Increased prevalence of diarrhea was observed among piglets fed the YP diet, whereas diarrhea was less prevalent among those fed CSE. Duodenal and jejunal villus height and duodenal crypt depth were greater in the control group than they were in the YP or CSE groups. Intraepithelial lymphocytes (IEL) in the duodenal and jejunal villi were enhanced by YP, whereas IEL in the ileal villi were reduced in weaned piglets fed YP. Secretion of jejunal and ileal interleukin-10 (IL-10) was higher and intestinal and serum antioxidant indexes were affected by YP and CSE. In YP-and CSE-supplemented animals, serum D-lactate concentration and diamine oxidase (DAO) activity were both increased, and intestinal mRNA expressions of occludin and ZO-1 were reduced as compared to the control animals. In conclusion, YP supplementation in the diets of weaned piglets appears to increase the incidence of diarrhea and has adverse effects on intestinal morphology and barrier function.
语种：
英文
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Absolute quantification of olive oil DNA by droplet digital-PCR (ddPCR): Comparison of isolation and amplification methodologies
作者：
Scollo, Francesco* ;Egea, Leticia A.;Gentile, Alessandra;La Malfa, Stefano;Dorado, Gabriel;...
期刊：
Food Chemistry ,2016年213:388-394 ISSN：0308-8146
通讯作者：
Scollo, Francesco
作者机构：
[Egea, Leticia A.; Scollo, Francesco; Hernandez, Pilar] CSIC, Inst Agr Sostenible IAS CSIC, Alameda Obispo S-N, Cordoba 14080, Spain.;[Scollo, Francesco; Gentile, Alessandra; La Malfa, Stefano] Univ Catania, Dipartimento Agr Alimentaz & Ambiente Di3A, Via Valdisavoia 5, I-95123 Catania, Italy.;[Dorado, Gabriel; Egea, Leticia A.] Univ Cordoba, Dept Bioquim & Biol Mol, Campus Rabanales C6-1-E17, E-14071 Cordoba, Spain.;[Gentile, Alessandra] Hunan Agr Univ, Hort & Landscape Coll, Changsha, Hunan, Peoples R China.
通讯机构：
[Scollo, Francesco] U;Univ Catania, Dipartimento Agr Alimentaz & Ambiente Di3A, Via Valdisavoia 5, I-95123 Catania, Italy.
关键词：
Molecular markers;Olea europaea;Olive oil;Traceability;qRT-PCR
摘要：
Olive oil is considered a premium product for its nutritional value and health benefits, and the ability to define its origin and varietal composition is a key step towards ensuring the traceability of the product. However, isolating the DNA from such a matrix is a difficult task. In this study, the quality and quantity of olive oil DNA, isolated using four different DNA isolation protocols, was evaluated using the qRT-PCR and ddPCR techniques. The results indicate that CTAB-based extraction methods were the best for unfiltered oil, while Nucleo Spin-based extraction protocols showed greater overall reproducibility. The use of both qRT-PCR and ddPCR led to the absolute quantification of the DNA copy number. The results clearly demonstrate the importance of the choice of DNA-isolation protocol, which should take into consideration the qualitative aspects of DNA and the evaluation of the amplified DNA copy number. © 2016
语种：
英文
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Repression of Biotin-Related Proteins by Benzo[a]Pyrene-Induced Epigenetic Modifications in Human Bronchial Epithelial Cells
作者：
Xia, Bo;Yang, Lin-Qing;Huang, Hai-Yan;Pang, Li;Yang, Xi-Fei;...
期刊：
International Journal of Toxicology ,2016年35(3):336-343 ISSN：1091-5818
通讯作者：
Zhuang, Zhi-Xiong;Liu, Jian-Jun
作者机构：
[Zhuang, ZX; Liu, Jian-Jun; Yang, Lin-Qing; Yang, Xi-Fei; Li, Jie; Zhuang, Zhi-Xiong; Huang, Hai-Yan; Ren, Xiao-Hu; Xia, Bo] Shenzhen Ctr Dis Control & Prevent, Key Lab Modern Toxicol, Shenzhen 518055, Peoples R China.;[Yi, You-Jin; Xia, Bo] Hunan Agr Univ, Coll Food Sci & Technol, Changsha, Hunan, Peoples R China.;[Pang, Li] Hunan Agr Univ, Coll Hort & Gardening, Changsha, Hunan, Peoples R China.
通讯机构：
[Zhuang, ZX; Liu, JJ] S;Shenzhen Ctr Dis Control & Prevent, Key Lab Modern Toxicol, Shenzhen 518055, Peoples R China.
关键词：
benzo[a]pyrene;DNA methylation;histone acetylation;biotinylation
摘要：
Benzo[a]pyrene (B[a]P) exposure has been associated with the alteration in epigenetic marks that are involved in cancer development. Biotinidase (BTD) and holocarboxylase synthetase (HCS) are 2 major enzymes involved in maintaining the homeostasis of biotinylation, and the deregulation of this pathway has been associated with a number of cancers. However, the link between B[a]P exposure and the dysregulation of BTD/HCS in B[a]P-associated tumorigenesis is unknown. Here we showed that the expression of both BTD and HCS was significantly decreased upon B[a]P treatment in human bronchial epithelial (16HBE) cells. Benzo[a]pyrene exposure led to the global loss of DNA methylation by immunofluorescence, which coincided with the reduction in acetylation levels on histones H3 and H4 in 16HBE cells. Consistent with decreased histone acetylation, histone deacetylases (HDACs) HDAC2 and HDAC3 were significantly upregulated in a dosage-dependent manner. When DNA methylation or HDAC activity was inhibited, we found that the reduction in BTD and HCS was separately regulated through distinct epigenetic mechanisms. Together, our results suggested the potential link between B[a]P toxicity and deregulation of biotin homeostasis pathway in B[a]P-associated cancer development.
语种：
英文
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Identification and validation of reference genes for quantitative real-time PCR studies in Hedera helix L.
作者：
Sun, Hua-peng;Li, Fang;Ruan, Qin-mei;Zhong, Xiao-hong*
期刊：
Plant Physiology and Biochemistry ,2016年108:286-294 ISSN：0981-9428
通讯作者：
Zhong, Xiao-hong
作者机构：
[Li, Fang; Sun, Hua-peng; Ruan, Qin-mei; Zhong, Xiao-hong] Hunan Agr Univ, Hort & Landscape Coll, Changsha 410128, Hunan, Peoples R China.;[Li, Fang] Natl Ctr Citrus Improvement, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Zhong, Xiao-hong] H;Hunan Agr Univ, Hort & Landscape Coll, Changsha 410128, Hunan, Peoples R China.
关键词：
*Abiotic stress;*Differential expression;*Hedera helix L.;*RT-qPCR;*Reference gene
摘要：
Reference gene evaluation and selection are necessary steps in gene expression analysis, especially in new plant varieties, through reverse transcription quantitative real-time PCR (RT-qPCR). Hedera helix L. is an important traditional medicinal plant recorded in European Pharmacopoeia. Research on gene expression in H. helix has not been widely explored, and no RT-qPCR studies have been reported. Thus, it is important and necessary to identify and validate suitable reference genes to for normalizing RT-qPCR results. In our study, 14 candidate protein-coding reference genes were selected. Their expression stability in five tissues (root, stem, leaf, petiole and shoot tip) and under seven abiotic stress conditions (cold, heat, drought, salinity, UV-C irradiation, abscisic acid and methyl jasmonate) were evaluated using geNorm and NormFinder. This study is the first to evaluate the stability of reference genes in H. helix. The results show that different reference genes should be chosen for normalization on the basis of various experimental conditions. F-box was more stable than the other selected genes under all analysis conditions except ABA treatment; 40S was the most stable reference gene under ABA treatment; in contrast, EXP and UBQ were the most unstable reference genes. The expressions of HhSE and Hhβ-AS, which are two genes related to the biosynthetic pathway of triterpenoid saponins, were also examined for reference genes in different tissues and under various cold stress conditions. The validation results confirmed the applicability and accuracy of reference genes. Additionally, this study provides a basis for the accurate and widespread use of RT-qPCR in selecting genes from the genome of H. helix.
语种：
英文
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Biotin-mediated epigenetic modifications: Potential defense against the carcinogenicity of benzo[a]pyrene
作者：
Xia, Bo;Pang, Li;Zhuang, Zhi-xiong* ;Liu, Jian-jun
期刊：
Toxicology Letters ,2016年241:216-224 ISSN：0378-4274
通讯作者：
Zhuang, Zhi-xiong
作者机构：
[Zhuang, Zhi-xiong; Liu, Jian-jun; Xia, Bo] Shenzhen Ctr Dis Control & Prevent, Lab Modern Toxicol, Med Key Lab Hlth Toxicol, Key Lab Modern Toxicol Shenzhen, Shenzhen 518060, Peoples R China.;[Xia, Bo] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Pang, Li] Hunan Agr Univ, Coll Hort & Gardening, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Zhuang, Zhi-xiong] S;Shenzhen Ctr Dis Control & Prevent, Lab Modern Toxicol, Med Key Lab Hlth Toxicol, Key Lab Modern Toxicol Shenzhen, Shenzhen 518060, Peoples R China.
关键词：
Benzo[a]pyrene;Biotin;Carcinogenicity;Epigenetic modification
摘要：
Environmental pollution and an unhealthy lifestyle result in direct exposure to dangerous chemicals that can modify endogenous pathways and induce malignant transformation of human cells. Although the molecular mechanisms of tumorigenesis are still not well understood, epigenetic alteration may be associated with exogenous chemical-induced carcinogenicity. Given the association between nutrition and cancer, nutrient supplementation may reduce aberrant epigenetic modifications induced by chemicals, thus decreasing carcinogenesis. This paper provides an overview of the epigenetic events caused by benzo[a] pyrene, a procarcinogenic and environmental pollutant, and biotin, an essential water-soluble vitamin, and investigates potential connections between them. This paper also discusses the potential inhibitory effect of biotin-related epigenetic modifications on the carcinogenicity of benzo [a] pyrene. The effect of nutritional supplementation on tumorigenesis involving epigenetic modifications is also discussed. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
语种：
英文
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Neuroprotective Effects of Theaflavins Against Oxidative Stress-Induced Apoptosis in PC12 Cells
作者：
Zhang, Jing;Cai, Shuxian;Li, Juan;Xiong, Ligui;Tian, Lili;...
期刊：
Neurochemical Research ,2016年41(12):3364-3372 ISSN：0364-3190
通讯作者：
Huang, Jianan;Liu, Zhonghua
作者机构：
[Zhang, Jing; Liu, Jianjun; Tian, Lili; Liu, Zhonghua; Xiong, Ligui; Huang, JN; Li, Juan; Huang, Jianan; Cai, Shuxian] Hunan Agr Univ, Minist Educ Tea Sci, Key Lab, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhonghua; Huang, JN; Huang, Jianan] Natl Res Ctr Engn Technol Utilizat Bot Funct Ingr, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhonghua; Huang, JN; Huang, Jianan] Collaborat Innovat Ctr Utilizat Funct Ingredients, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Huang, JN; Liu, ZH] H;[Huang, JN; Liu, ZH] N;[Huang, JN; Liu, ZH] C;Hunan Agr Univ, Minist Educ Tea Sci, Key Lab, Changsha 410128, Hunan, Peoples R China.;Natl Res Ctr Engn Technol Utilizat Bot Funct Ingr, Changsha 410128, Hunan, Peoples R China.
关键词：
Theaflavin;Neuroprotective effect;Oxidative stress;Apoptosis;PC12 cells
摘要：
Oxidative stress can induce neuronal apoptosis via the production of superoxide and hydroxyl radicals. This process is as a major pathogenic mechanism in neurodegenerative disorders. In this study, we aimed to clarify whether theaflavins protect PC12 cells from oxidative stress damage induced by H 2 O 2 . A cell model of PC12 cells undergoing oxidative stress was created by exposing cells to 200 μM H 2 O 2 in the presence or absence of varying concentrations of theaflavins (5, 10, and 20 μM). Cell viability was monitored using the MTT assay and Hoechst 33258 staining, showing that 10 μM theaflavins enhanced cell survival following 200 μM H 2 O 2 induced toxicity and increased cell viability by approximately 40 %. Additionally, we measured levels of intracellular reactive oxygen species (ROS) and antioxidant enzyme activity. This suggested that the neuroprotective effect of theaflavins against oxidative stress in PC12 cells is derived from suppression of oxidant enzyme activity. Furthermore, Western blot analyses indicated that theaflavins downregulated the ratio of pro-apoptosis/anti-apoptosis proteins Bax/Bcl-2. Theaflavins also downregulated the expression of caspase-3 compared with a H 2 O 2 -treated group that had not been treated with theaflavins. Interestingly, this is the first study to report that the four main components of theaflavins found in black tea can protect neural cells (PC12) from apoptosis induced by H 2 O 2 . These findings provide the foundations for a new field of using theaflavins or its source, black tea, in the treatment of neurodegenerative diseases caused by oxidative stress. © 2016, Springer Science+Business Media New York.
语种：
英文
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Molecular cloning and characterization of a flavanone 3-Hydroxylase gene from Artemisia annua L.
作者：
Xiong, Shuo;Tian, Na;Long, Jinhua;Chen, Yuhong;Qin, Yu;...
期刊：
Plant Physiology and Biochemistry ,2016年105:29-36 ISSN：0981-9428
通讯作者：
Xiao, Wenjun;Liu, Shuoqian
作者机构：
[Tian, Na; Xiong, Shuo; Xiao, Wenjun; Liu, Shuoqian; Chen, Yuhong; Feng, Jinyu; Xiao, WJ; Qin, Yu] Hunan Agr Univ, Coll Hort & Hardening, Hunan Collaborat Innovat Utilizat Bot Funct Ingre, Changsha 410128, Hunan, Peoples R China.;[Tian, Na; Xiao, Wenjun; Liu, Shuoqian; Long, Jinhua] Natl Res Ctr Engn Technol Utilizat Funct Ingredie, Changsha, Hunan, Peoples R China.;[Liu, Shuoqian] Minist Educ, Key Lab Tea Sci, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Xiao, WJ; Liu, SQ] H;Hunan Agr Univ, Coll Hort & Hardening, Hunan Collaborat Innovat Utilizat Bot Funct Ingre, Changsha 410128, Hunan, Peoples R China.
关键词：
Artemisia annua L.;Dihydrokaempferol;Flavanone 3-hydroxylase;Flavonoids;Naringenin
摘要：
Flavonoids were found to synergize anti-malaria and anti-cancer compounds in Artemisia annua, a very important economic crop in China. In order to discover the regulation mechanism of flavonoids in Artemisia annua, the full length cDNA of flavanone 3-hydroxylase (F3H) were isolated from Artemisia annua for the first time by using RACE (rapid amplification of cDNA ends). The completed open read frame of AaF3H was 1095 bp and it encoded a 364-amino acid protein with a predicted molecular mass of 41.18 kDa and a pI of 5.67. The recombinant protein of AaF3H was expressed in E. coli BL21(DE3) as His-tagged protein, purified by Ni-NTA agrose affinity chromatography, and functionally characterized in vitro. The results showed that the His-tagged protein (AaF3H) catalyzed naringenin to dihydrokaempferol in the present of Fe2+. The Km for naringenin was 218.03 mu M. The optimum pH for AaF3H reaction was determined to be pH 8.5, and the optimum temperature was determined to be 35 degrees C. The AaF3H transcripts were found to be accumulated in the cultivar with higher level of flavonoids than that with lower level of flavonoids, which implied that AaF3H was a potential target for regulation of flavonoids biosynthesis in Artemisia annua through metabolic engineering. (C) 2016 Elsevier Masson SAS. All rights reserved.
语种：
英文
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Ethanolamine enhances the proliferation of intestinal epithelial cells via the mTOR signaling pathway and mitochondrial function
作者：
Yang, Huansheng;Xiong, Xia;Li, Tiejun;Yin, Yulong*
期刊：
In Vitro Cellular & Developmental Biology - Animal ,2016年52(5):562-567 ISSN：1071-2690
通讯作者：
Yin, Yulong
作者机构：
[Xiong, Xia; Li, Tiejun; Yin, Yulong; Yang, Huansheng] Chinese Acad Sci, Observat & Expt Stn Anim Nutr & Feed Sci South Ce, Minist Agr, Yuandaer Rd 644, Changsha 410125, Hunan, Peoples R China.;[Xiong, Xia; Li, Tiejun; Yin, Yulong; Yang, Huansheng] Chinese Acad Sci, Hunan Prov Engn Res Ctr Hlth Livestock & Poultry, Yuandaer Rd 644, Changsha 410125, Hunan, Peoples R China.;[Xiong, Xia; Li, Tiejun; Yin, Yulong; Yang, Huansheng] Chinese Acad Sci, Key Lab Agroecol Proc Subtrop Reg, Inst Subtrop Agr, Yuandaer Rd 644, Changsha 410125, Hunan, Peoples R China.;[Yin, Yulong; Yang, Huansheng] Hunan Normal Univ, Sch Life Sci, Changsha, Hunan, Peoples R China.;[Xiong, Xia; Yin, Yulong] Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Bot Funct Ingr, Prov Coinnovat Ctr Utilizat Bot Funct Ingredients, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Yin, Yulong] C;[Yin, Yulong] H;Chinese Acad Sci, Observat & Expt Stn Anim Nutr & Feed Sci South Ce, Minist Agr, Yuandaer Rd 644, Changsha 410125, Hunan, Peoples R China.;Chinese Acad Sci, Hunan Prov Engn Res Ctr Hlth Livestock & Poultry, Yuandaer Rd 644, Changsha 410125, Hunan, Peoples R China.;Chinese Acad Sci, Key Lab Agroecol Proc Subtrop Reg, Inst Subtrop Agr, Yuandaer Rd 644, Changsha 410125, Hunan, Peoples R China.
关键词：
Ethanolamine;Intestine;Epithelial cells;Proliferation;mTOR signaling pathway
摘要：
Ethanolamine (Etn), which is the base constituent of phosphatidylethanolamine, a major phospholipid in animal cell membranes, is required for the proliferation of many types of mammalian epithelial cells. However, it is not clear whether the proliferation of intestinal epithelial cells requires Etn. The present study was conducted to examine the effects of Etn on the proliferation of intestinal epithelial cells and to elucidate the underlying mechanisms. The addition of Etn at 100 or 200 μM was found to enhance the proliferation of IPEC-1 cells. The expression of cell cycle-related proteins CDK4, RB3, cyclin A, and PCNA was also enhanced by Etn. Moreover, the expression or phosphorylation levels of the mammalian target of rapamycin (mTOR) signaling pathway protein and the expression of proteins related to mitochondrial function were also affected by Etn in IPEC-1 cells. These results indicate that Etn promotes the proliferation of intestinal epithelial cells by exerting effects on mTOR signaling pathway and mitochondrial function. © 2016, The Society for In Vitro Biology.
语种：
英文
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Effects of GA3 and CPPU on grape fruit adjacent leaf photosynthesis and fruit quality
作者：
辛守鹏;刘帅;余阳;聂松青;高志红;...
期刊：
应用生态学报 ,2015年26(6):1814-1820 ISSN：1001-9332
通讯作者：
Tao, J.-M.
作者机构：
[余阳; 陶建敏; 辛守鹏; 高志红; 刘帅] College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China;[聂松青] College of Horticulture and Landscape, Hunan Agricultural University, Changsha, 410128, China
通讯机构：
[Tao, J.-M.] C;College of Horticulture, China
关键词：
葡萄;光响应曲线;果实品质
摘要：
以‘阳光玫瑰’葡萄为材料,在连栋避雨大棚栽培条件下,研究了花后两周同一浓度赤霉素(GA3)与不同浓度细胞分裂素(CPPU)组合对葡萄果穗邻近叶片的光合特性及果实品质的影响.结果表明:非直角双曲线模型较为适合果穗邻近叶光响应曲线拟合.在25 mg·L-1GA3分别与5、10、15、20 mg·L-1CPPU组合处理下,葡萄果穗邻近叶的净光合速率和气孔导度均随着光合有效辐射的增加而增加,胞间CO2浓度降低.25 mg·L-1GA3分别与5、10、15mg·L-1CPPU组合处理下,果实品质随着CPPU浓度的增加而提高.25 mg·L-1 GA3+20mg · L-1CPPU处理下,虽然果穗邻近叶的光合能力高于其他浓度处理,但果实品质相关指标较25 mg·L-1GA3+15 mg·L-1CPPU处理时低,表明适宜的CPPU浓度可以提高果实邻近叶的光合能力及果实品质,浓度过高反而使果实品质下降.花后两周较为合理的组合为25mg·L-1 GA3+15 mg·L-1CPPU.
语种：
中文
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