作者：
Gang Chen;Jun Zhang;Tiejun Li;Lixiang Chen;Yulong Yin;...
作者机构：
[Gang Chen; Jun Zhang; Tiejun Li; Yulong Yin; Guoyao Wu] Chinese Acad Sci,Inst Subtrop Agr,Res Ctr Healthy Breeding Livestock & Poultry,Hunan Engn& Res Ctr Anim & Poultry Sci,Key Lab Agroecol Proc Subtrop Reg,Scientific Oberving and Experimetal Station of Animal Nutrition and Feed Science in South-Central,Ministryof Agriculture,Changsha City,Furong Road ＃644,Hunan,Peoples R China 410125;[Gang Chen; Lixiang Chen] College of Animal Science and Technology,Hunan Agriculture University,Changsha,China 410128;[Guoyao Wu] Department of Animal Science,Texas A&M University,College Station,TX 77843,US
会议名称：
第六届动物营养、保健与饲料添加剂国际学术研讨会
会议时间：
2013-7-22
会议地点：
青岛
会议主办单位：
国家生猪产业技术创新战略联盟
会议论文集名称：
第六届动物营养、保健与饲料添加剂国际学术研讨会论文集
关键词：
monosodium glutamate;suckling pig;liver;protein and lipid metabolism;gene expression
摘要：
The experiment was conducted to investigate the effects of monosodium glutamate on protein、lipid metabolism of suckling piglets.A total of 24(6 nests,4 piglets/nest) crossbred (Duroc×Large White×Landrace) newborn pigs were selected,each nest were randomly divided to four treatments: The doses of MSG were 0.06(Low-MSG,n=6),0.5,(Medium-MSG,n=6),and lg (High-MSG,n=6)per kg of body weight per day.The MSG was dissolved in 2 ml water per kg of body weight before gavage.Control piglets (CN,n=6) received the same amount of sodium as NaCl in the middle group(0.24g NaCl).Monosodium glutamate was dissolved in water based on weight,force-feeding at 8:00 and 18:00 everyday.The results showed that,concentration of total protein(TP)in the serum of the middle dose group was significantly higher than that in the control group (P<0.05);the there was no significant difference(P>0.05)of serum urea nitrogen(UN、UREA) between each group; compared with the control group,serum triglyceride(TG),total cholesterol (TC),low density lipoprotein cholesterol(LDL-C)concentration had no significant difference(P>0.05);high density lipoprotein cholesterol(HDL-C)concentrations in serum of the middle dose group were significantly higher than those in the control group(P<0.05).Compared with the control group,low and middle dose of MSG could significantly improve the protein content(P<0.01) in the longissimus muscle of suckling piglets; middle dose of MSG can improve the fat content in the longissimus muscle of suckling piglets significantly(P<0.05).Compared with the control group,There was significant difference(P<0.01 )expression of IGF-1、GOT、GPT in liver of middle dose group; there was no significant diflference(P>0.05)expression of IGF-1 of middle and high dose group; GOT mRNA expression of low and high dose group significantly(P<0.05)higher than the control group; GPT mRNA expression of middle dose group raise significantly(P<0.05),whereas reduced significantly (P<0.05)of high dose group.With the additional dose increased.Compared with the control group,0.25g/kg/time monosodium glutamate significantly(P<0.05)raise the ACC、FAS mRNA expression in liver,whereas no significant diflference(P>0.01 )was observed in low and high dose group; MSG supplementation did not affect mRNA expression for HSL or CPT-1(P>0.05).These results suggest that 0.25g/kg/time monosodium glutamate additive improve the protein synthesis and sediment of suckling pig;0.25g/kg/time monosodium glutamate additive increase the lipid sediment through promote lipid synthesis and maintain the break level previously.
摘要：
The antimicrobial peptide buforin II contains residues Thr16 to Lys36 of buforin I and exhibits antimicrobial activity that is twice as potent as that of its parent peptide. Buforin II was expressed in Pichia pastoris FZM(2009) as a fusion peptide linked to porcine interferon-a and tested as an alternative to antimicrobial growth-promoters in pig production. Fifteen Landrace x Yorkshire barrows (5.55 +/- 0.49 kg), weaned at 21 days of age, were challenged with three enterotoxigenic Escherichia coli strains. The animals were randomly divided into 3 groups with 5 barrows in each, fed a maize-soybean meal diet, and orally dosed with 5 mL sterile water (CON), 5 mL buforin II (BF; 0.05 mg/mL in sterile water), or 5 mL colistin sulphate (CS; 0.5 mg/mL in sterile water) twice daily for 21 days. Compared with CS and CON, oral administration of BF increased (P<0.05) daily weight gain, feed intake gain, and feed conversion. The expression of tight junction proteins and protective factors in the small intestine also increased in BF-treated piglets. Compared with the CON group, oral administration of BF and CS decreased (P<0.05) for the abundance of hemolytic E. coli in rectal swabs. Collectively, our results indicate that oral administration of buforin II protects small intestinal mucosal membrane integrity by increasing the abundance of tight junction proteins and enhancing the expression of protective factors, and can reduce hemolytic E. coli concentrations in the intestines of piglets. (C) 2013 Elsevier B.V. All rights reserved.
