期刊：
Journal of Veterinary Pharmacology and Therapeutics,2009年32:220-221 ISSN：0140-7783
作者机构：
[Chrzistek, K.; Suszko, A.; Szczypka, M.; Lis, M.; Switala, M.; Pozniak, B.; Obminska-Mrukowicz, B.] Wroclaw Univ Environm & Life Sci, Fac Vet Med, Wroclaw, Poland.;[Yi, J.] Hunan Agr Univ, Coll Vet Med, Changsha, Hunan, Peoples R China.
期刊：
EPIDEMIOLOGY AND INFECTION,2008年136(4):504-508 ISSN：0950-2688
通讯作者：
Tu, C.
作者机构：
[Jiang, Y.; Hu, Z.; Tu, C.; Wang, L.] Acad Mil Med Sci, Mil Vet Res Inst, Changchun 130062, Peoples R China.;[Jiang, Y.] Jilin Univ, Coll Anim Sci & Vet Med, Changchun 130023, Peoples R China.;[Yu, X.; Liu, H.] Hunan Agr Univ, Vet Coll, Changsha, Hunan, Peoples R China.;[Lu, Z.] Foshan Univ, Vet Coll, Foshang, Peoples R China.;[Xuan, H.] Guangdong Acad Agr Sci, Inst Vet Med, Guangzhou, Guangdong, Peoples R China.
通讯机构：
[Tu, C.] A;Acad Mil Med Sci, Mil Vet Res Inst, 1068 Qinglong Rd, Changchun 130062, Peoples R China.
关键词：
Swine;Rabies;China;Dogs;personal identity
摘要：
The first dog-associated outbreak of rabies in swine in China (Hunan province) has been diagnosed and the related virus isolated. Sequence analysis showed that the pig isolate was a genotype 1 rabies virus with a very high nucleotide identity to local dog isolates.
通讯机构：
[Xiao, HB ] ;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
关键词：
6-tetrahydroxyxanthone;angiopoietin-like protein3;lipoprotein lipase;total cholesterol;triglyceride;high-density lipoprotein cholesterol;apolipoproteinE-deficient mice;6-tétrahydroxyxanthone;protéine de type angiopoïétine 3;lipoprotéine lipase;cholestérol total;triglycéride;cholestérol de la lipoprotéine de haute densité;souris déficientes en apolipoprotéine E
摘要：
Improvement of animal embryo sexing depends upon high-titer serologically detected male (SDM) antibody fragments. SDM sera collected from isogenic C57BL/7 female mice after inoculation with male spleen cells were characterized and used for construction of a recombinant Fab antibody library against SDM antigen, and used for analysis of the binding capacity and specificity to SDM antigen. The heavy-chain Fd and full-length light-chain K were amplified by RT-PCR from a mouse (#6) that'ed high-titer antiserum. The amplified product was inserted into the pComb3 vector followed by co-infections with the help phage VCSM 13 for construction of the phage library, which gave 1.5 x 10(7) colonies with the titer of 3.2 x 10(11) pfu/ml by a recombination rate of 80%. Sequence analysis of the PCR products of plasmid DNA of E5 clones showed that V-H and V-k had common characteristics shared by other known variable region of antibodies. The Fab antibody libraries against SDM antigen were enriched by three cycles of affinity enrichment with male spleen cells, and two cycles of non-specific absorption with female spleen cells. The ELISA results showed that 9 of 15 clones had binding capacity to the SDM antigen. This is the first report on a phage display library of SDM antigen. The mouse Fab antibody library could be used for identifying SDM antigen, and for the development of sex determination of early embryos in mammals. (C) 2007 Elsevier B.V. All rights reserved.
期刊：
Pesticide Biochemistry and Physiology,2007年89(2):146-150 ISSN：0048-3575
通讯作者：
Qiu, Xinghui
作者机构：
[Qiu, Xinghui] Chinese Acad Sci, Inst Zool, State Key Lab Integrated Management Pest Insects, Beijing 100101, Peoples R China.;Hunan Agr Univ, Anim Med Coll, Changsha 410128, Peoples R China.
通讯机构：
[Qiu, Xinghui] C;Chinese Acad Sci, Inst Zool, State Key Lab Integrated Management Pest Insects, Beijing 100101, Peoples R China.
摘要：
We investigated the molecular basis of resistance in a strain of house fly (BJD) from Beijing, China. This strain showed 567-fold resistance to commonly used deltamethrin. Flies were 64-fold resistant to deltamethrin synergized by piperonyl butoxide (PBO). The 5'-flanking sequence of the cytochrome P450 gene CYP6D1 in BJD strain had a 15-bp insert as in the LPR strain. Two mutations (kdr, super-kdr) in the voltage sensitive sodium channel (VSSC) were also detected in the BJD strain. Our results showed that a combination of resistance alleles for CYP6D1 and VSSC existed in deltamethrin resistant house flies in China. (C) 2007 Elsevier Inc. All rights reserved.
摘要：
This paper aimed to study the toxicity of arsanilic acid on rat primary hepatocytes in vitro by a modification of the perfusion method. The conditions included concentrations of 0, 1.085, 10.85, 108.5, 1,085 and 10,850 mg/kg arsanilic acid in RPMI 1,640 medium at rat hepatocytes plates respectively, each group had five repeats at 37°C for 48 h. The rat primary hepatocytes survival ratio, DNA Ladder, activities of glutathione peroxidase (GSH-px), superoxide dismutase (SOD) and catalase (CAT) in hepatocytes, activity of SOD in the medium and the expression of gene bax in hepatocytes were measured at 12 h, 24 h and 48 h respectively. The results showed that arsanilic acid decreased the activities of GSH-px and SOD, and increased the activity of CAT in all dosages, and affected as positive DNA ladder. Although the SOD activities of both hepatocytes and medium in 1.085 mg/L arsanilic acid were significantly lower than the base line at 12 h, CAT activity in 10.85 mg/L arsanilic acid was significantly higher than the base line at 48 h, and all of the DNA ladders were positive, which means 1.085 mg/L arsanilic acid induced apoptosis at 24 h. The gene expression of bax was significantly upregulated in 1.085 mg/L arsanilic acid or higher for 24 h. The parameters in 1,085 mg/L and 10,850 mg/L arsanilic acid had more severe changes than the others at any time indicating that these levels of arsanilic acid were toxic hazards for hepatocyte survival. It was concluded that arsanilic acid induced a dosage- and time-dependent gene expression of bax, 1.085 mg/L arsanilic acid could be involved in rat liver cell apoptosis at 24 h. Arsanilic acid as additives in livestock feed could present potential toxic implications for farm animals.
