作者：
Xiao, Hong-Bo*;Lu, Xiang-Yang;Zhang, Heng-Bo;Sun, Zhi-Liang;Fang, Jun
期刊：
Journal of Physiology and Biochemistry,2013年69(4):719-725 ISSN：1138-7548
通讯作者：
Xiao, Hong-Bo
作者机构：
[Xiao, Hong-Bo; Sun, Zhi-Liang] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Lu, Xiang-Yang] Hunan Agr Bioengn Res Inst, Changsha 410128, Hunan, Peoples R China.;[Zhang, Heng-Bo] Furong Dist Red Cross Hosp, Changsha 410126, Hunan, Peoples R China.;[Sun, Zhi-Liang] Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Biol Vet Drugs Branch, Changsha 410128, Hunan, Peoples R China.;[Fang, Jun] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Xiao, Hong-Bo] H;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
关键词：
Undaria pinnatifida;Soluble fiber;Angiopoietin-like protein 3;Hyperlipidemia;Mice
摘要：
Angiopoietin-like protein 3 (Angptl3)-lipoprotein lipase (LPL) pathway may be a useful pharmacologic target for hyperlipidemia. The present study was conducted to test the effect of soluble fiber extracted from Undaria pinnatifida (UP), on hyperlipidemia in apolipoprotein E-deficient (ApoE(-/-)) mice. Forty mice were divided into four groups (n = 10): control group (C57BL/6J mice), ApoE(-/-) mice group, and two groups of ApoE(-/-) mice treated with UP fiber (5 or 10 % per day). UP soluble fiber treatment significantly decreased plasma and hepatic total cholesterol, triglycerides levels, plasma low-density lipoprotein cholesterol, and malondialdehyde concentrations and increased plasma high-density lipoprotein cholesterol level and downregulated protein expression of Angptl3 concomitantly with upregulated protein expression of LPL. In addition, T0901317 caused elevated expression of hepatic Angptl3 protein, and the effect of T0901317 was also abrogated by UP soluble fiber in C57BL/6J mice. The present results suggest that the UP soluble fiber regulates Angptl3-LPL pathway to lessen hyperlipidemia in mice.
作者机构：
[Song, Hui-Qun; Lin, Rui-Qing; Zhang, Yuan; Liu, Guo-Hua; Zhu, Xing-Quan] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, Key Lab Vet Parasitol Gansu Prov, State Key Lab Vet Etiol Biol, Lanzhou 730046, Gansu, Peoples R China.;[Yuan, Zi-Guo; Lin, Rui-Qing; Zhang, Yuan; Weng, Ya-Biao] S China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China.;[Liu, Guo-Hua] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Li, Ming-Wei] Guangdong Ocean Univ, Coll Agr, Zhanjiang 524088, Guangdong, Peoples R China.;[Zhu, Xing-Quan; Zou, Feng-Cai] Yunnan Agr Univ, Coll Anim Sci & Technol, Kunming 650201, Yunnan Province, Peoples R China.
通讯机构：
[Zhu, Xing-Quan] C;Chinese Acad Agr Sci, Lanzhou Vet Res Inst, Key Lab Vet Parasitol Gansu Prov, State Key Lab Vet Etiol Biol, Lanzhou 730046, Gansu, Peoples R China.
摘要：
In this study, sequence variation in three mitochondrial DNA regions, namely cytochrome c oxidase subunit (cox1) and NADH dehydrogenase subunits 1 and 4 (nad1 and nad4), between Oesophagostomum dentatum and O. quadrispinulatum isolated from pigs in different geographical origins in Mainland China was examined, and their phylogenetic relationships were reconstructed. A partial of the cox1 (pcox1), nad1, and nad4 genes (pnad1 and pnad4) were amplified separately from individual nodule worms by PCR and were subjected to direct sequencing in order to define sequence variations. While the intraspecific sequence variations within each of the two species were 0.3-5.2% for pcox1, 0-4.9% for pnad1, and 0-7.1% for pnad4, the interspecific sequence differences were significantly higher, being 10.7-13.4% for pcox1, 11-14.6% for pnad1, and 14.9-18% for pnad4, respectively. There were a number of nucleotide positions in the pcox1, pnad1, and pnad4 sequences with no apparent intraspecific variation but distinct interspecific differences among those samples of Oesophagostomum spp. examined, which may be used as genetic makers for the identification and differentiation of the Oesophagostomum spp. Phylogenetic analyses using three inference methods, namely Bayesian inference, maximum likelihood, and maximum parsimony based on the combined sequences of pcox1, pnad1, and pnad4 revealed that the O. dentatum and O. quadrispinulatum form monophyletic groups, respectively. These findings demonstrated clearly the usefulness of the three mitochondrial sequences for studying systematics, population genetic structures, and the molecular ecology of Oesophagostomum spp.
作者机构：
[Song, Hui-Qun; Lin, Rui-Qing; Liu, Guo-Hua; Zhu, Xing-Quan] Chinese Acad Agr Sci, State Key Lab Vet Etiol Biol, Lanzhou Vet Res Inst, Key Lab Vet Parasitol Gansu Prov, Lanzhou 730046, Gansu, Peoples R China.;[Lin, Rui-Qing; Zhang, Yuan] S China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China.;[Liu, Guo-Hua; Zhu, Xing-Quan] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Hu, Min] Huazhong Agr Univ, Coll Vet Med, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China.;[Wu, Xiang-Yun] S China Sea Inst Oceanol, Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China.
通讯机构：
[Zhu, Xing-Quan] C;Chinese Acad Agr Sci, State Key Lab Vet Etiol Biol, Lanzhou Vet Res Inst, Key Lab Vet Parasitol Gansu Prov, Lanzhou 730046, Gansu, Peoples R China.
摘要：
A Gram-negative, rod-shaped bacterial strain named PB-CS01 which is bioluminescence-positive was isolated from contaminated commercial pork that probably had been exposed to seafood. Optimal growth of strain PB-CS01 requires the presence of 3.0% (w/v) NaCl and a temperature of 20 degrees C. Phylogenetic analysis based on 16S rRNA gene sequences of strain PB-CS01 and other Photobacterium species showed that the novel isolate belongs to the genus Photobacterium. Sequence similarity analysis between PB-CS01 and other species indicates that the closest relatives of strain PB-CS01 are Photobacterium phosphoreum ATCC 11040 (99.9%), Photobacterium kishitanii pjapo.1.1 (99.8%) and Photobacterium iliopiscarium ATCC 51760 (99.5%). The most abundant fatty acids were summed feature 3 (50.77%; C-16:1 omega 7c and/or C-16:1 omega 6c) and C-16:0 (15%). The fatty acid profile is similar to that of the genus Photobacterium but this report is the first to describe C-16:1 omega 6c as one of the compositions of summed feature 3 of the genus Photobacterium. The G+C content of the genomic DNA of strain. PB-CS01 was 44.8 mol%. Overall, strain. PB-CS01 is a novel Photobacterium species.
作者机构：
[Xu, Min-Jun; Su, Ang; Liu, Guo-Hua; Zhu, Xing-Quan] Chinese Acad Agr Sci, State Key Lab Vet Etiol Biol, Key Lab Vet Parasitol Gansu Prov, Lanzhou Vet Res Inst, Lanzhou, Gansu, Peoples R China.;[Liu, Guo-Hua; Zhu, Xing-Quan] Hunan Agr Univ, Coll Vet Med, Changsha, Hunan, Peoples R China.;[Gasser, Robin B.] Univ Melbourne, Dept Vet Sci, Werribee, Vic 3030, Australia.;[Su, Ang; Lin, Rui-Qing] S China Agr Univ, Coll Vet Med, Guangzhou, Guangdong, Peoples R China.;[Nejsum, Peter] Univ Copenhagen, Dept Vet Dis Biol, Frederiksberg, Denmark.
通讯机构：
[Liu, Guo-Hua] C;Chinese Acad Agr Sci, State Key Lab Vet Etiol Biol, Key Lab Vet Parasitol Gansu Prov, Lanzhou Vet Res Inst, Lanzhou, Gansu, Peoples R China.
摘要：
Author Summary Trichuriasis is a neglected tropical disease (NTD) caused by parasitic nematodes of the genus Trichuris (Nematoda), causing significant human and animal health problems as well as considerable socio-economic consequences world-wide. Although Trichuris species are considered to be relatively host specific, there has been significant controversy as to whether Trichuris infecting humans (recognized as T. trichiura) is a distinct species from that found in pigs (recognized as T. suis), or not. In the present study, we sequenced, annotated and compared the complete mitochondrial genomes of Trichuris from these two hosts and undertook a phylogenetic analysis of the mitochondrial datasets. This analysis showed clear genetic distinctiveness and strong statistical support for the hypothesis that T. trichiura and T. suis are separate species, consistent with previous studies using nuclear ribosomal DNA sequence data. Future studies could explore, using mitochondrial genetic markers defined in the present study, cross-transmission of Trichuris between pigs and humans in endemic regions, and the population genetics of T. trichiura and T. suis.
作者机构：
[Bai Xia; Xiao Hong-bo; Mai Pei; Sun Zhi-liang; Wang Shui-lian; Liu Zhao-ying; Dong Wei; Chen Xiao-jun; Wang Hui; Cai Jie] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Bai Xia; Xiao Hong-bo; Mai Pei; Sun Zhi-liang; Liu Zhao-ying; Dong Wei; Chen Xiao-jun; Wang Hui; Cai Jie] Engn Res Ctr Vet Drugs Hunan Prov, Changsha 410311, Hunan, Peoples R China.
通讯机构：
[Sun Zhi-liang] H;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
关键词：
angiopoietin-like protein 3;clone;pregnancy toxemia;goat
摘要：
Pregnancy toxemia is a metabolic disorder of lipid and glucose. Recent investigations have found that angiopoietin-like protein 3 (ANGPTL3) can contribute to disorder of carbohydrate and lipid metabolism. The present study was conducted to investigate the change of ANGPTL3 expression during pregnancy toxemia. We firstly cloned the full-length cDNA of ANGPTL3 in Liuyang Black goats, revealing that goat ANGPTL3 had the typical structure of the angiopoietin-like family, and its mRNA was exclusively expressed in liver. Pregnancy toxemia of pregnant goat does with twins during late gestation was induced by being fasted for 72 h, and then they were recovered after 5 d of refeeding. Hepatic ANGPTL3 gene expression was significantly down-regulated concomitantly with decreased serum glucose concentration, elevated serum β-hydroxybutyrate and free fatty acid levels in pregnant toxemic goats, and these changes were reversed after refeeding. These results suggest ANGPTL3 may play a certain role in the development of pregnancy toxemia in goats.
摘要：
This study was conducted to test the hypotheses that dietary arginine and glutamine supplementation may confer beneficial effect on porcine circovirus type 2 (PCV2)-infected pigs. The measured variables included: 1) average daily weight gain (ADG), feed conversion rate (FCR); 2) PCV2 virus load in the submaxillary lymph node, spleen, liver, kidney and heart at 20 days post infection (DPI); 3) microscopic lesions in the spleen at 20 DPI; 4) serum cytokine levels, serum antioxidant capacity (T-AOC, T-SOD and GSH-PX), serum PCV2 virus load, serum nitric oxide (NO) and serum PCV2 antibody titers at 5, 10, 15 and 20 DPI. Dietary arginine and glutamine supplementation conferred positive effect on the production of PCV2 infected pigs. Meanwhile, arginine and glutamine supplementation increased the antioxidant capacity and immune response, and decreased the PCV2 virus load in tissue. Moreover, these beneficial effects may be mainly performed through NO. It was concluded that dictary, arginine and glutamine supplementation confers positive effect on the PCV2 infected pigs.
摘要：
For many years, whipworms (Trichuris spp.) have been described with a relatively narrow range of both morphological and biometrical features. Moreover, there has been insufficient discrimination between congeners (or closely related species). In the present study, we determined the complete mitochondrial (mt) genomes of two whipworms Trichuris ovis and Trichuris discolor, compared them and then tested the hypothesis that T. ovis and T. discolor are distinct species by phylogenetic analyses using Bayesian inference, maximum likelihood and maximum parsimony) based on the deduced amino acid sequences of the mt protein-coding genes. The complete mt genomes of T. ovis and T. discolor were 13,946 bp and 13,904 bp in size, respectively. Both mt genomes are circular, and consist of 37 genes, including 13 genes coding for proteins, 2 genes for rRNA, and 22 genes for tRNA. The gene content and arrangement are identical to that of human and pig whipworms Trichuris trichiura and Trichuris suis. Taken together, these analyses showed genetic distinctiveness and strongly supported the recent proposal that T. ovis and T. discolor are distinct species using nuclear ribosomal DNA and a portion of the mtDNA sequence dataset. The availability of the complete mtDNA sequences of T. ovis and T. discolor provides novel genetic markers for studying the population genetics, diagnostics and molecular epidemiology of T. ovis and T. discolor. (C) 2012 Elsevier B.V. All rights reserved.
作者机构：
[Li, Chuanfeng; Fu, Yuzhi; Chen, Zongyan; Liu, Guangqing] Chinese Acad Agr Sci, Shanghai Vet Res Inst, Shanghai 200241, Peoples R China.;[Fu, Yuzhi] Hunan Agr Univ, Anim Med Coll, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liu, Guangqing] C;Chinese Acad Agr Sci, Shanghai Vet Res Inst, Shanghai 200241, Peoples R China.
关键词：
Suicidal DNA vaccine;Duck hepatitis virus;VP1 gene;Ducklings
摘要：
A suicidal DNA vaccine based on a Semliki Forest virus (SFV) replicon was evaluated for the development of a vaccine against duck hepatitis virus type 1 (DHV-1). The VP1 gene of DHV-1 was cloned and inserted into pSCA1, an SFV DNA-based replicon vector. The resultant plasmid, pSCA/VP1, was transfected into BHK-21 cells and the antigenicity of the expressed protein was confirmed using an indirect immunofluorescence and western blot assay. Immunogenicity was studied in ducklings. Ducklings were injected intramuscularly two times with pSCA/VP1 at 14 days intervals. Anti-DHV-1 antibodies were detected by ELISA, the lymphocyte proliferation response was also tested by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide method and neutralizing antibodies were measured by microneutralization tests. Our results showed that DHV-1-specific antibodies, neutralizing antibodies and lymphocyte proliferation were well induced in ducklings. Furthermore, all the ducklings were protected against challenge with wild DHV-1. In conclusion, we demonstrate that the suicidal DNA vaccine is a promising vaccine candidate facilitating the prevention of duck hepatitis caused by DHV-1.
摘要：
Gossypol is a yellow polyphenolic compound from cotton plant. It has been shown to exert a potential for anti-cancer, anti-inflammatory and anti-fertility effects. Following effects of gossypol acetic acid (GA) in mice were determined in the present study: influence on the total number of lymphocytes, weight ratio and subsets of lymphocytes in the thymus, spleen and mesenteric lymph nodes, and humoral immune response to sheep erythrocytes (SRBC). Gossypol acetic acid was administered orally four times at 24 h intervals at three doses of 5.0, 25.0 and 125.0 mg/kg to SRBC-immunized mice (prior to or after priming), and non-immunized mice. It was found that GA decreased the total number of lymphocytes in thymus and mesenteric lymph nodes. Gossypol acetic acid dependent on the dosage decreased the percentage and absolute count of CD4+ thymocytes but increased the percentage and absolute count of CD8+ lymphocytes in spleen and lymph nodes. In SRBC-immunized mice, GA at the doses of 25.0 and 125.0 mg/kg administered prior to SRBC reduced the number of plaque forming cells (PFC) and the production of IgG. However, GA administered after priming decreased the production of IgM and IgG. In vitro, GA inhibited proliferation of J774.E murine macrophages in a dose- and time-dependent manner.
