通讯机构：
[Yu, Xing-Long] H;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
关键词：
Defective interfering RNA;Porcine reproductive and respiratory syndrome virus
摘要：
In the present study, seven new defective interfering (DI) RNA species of porcine reproductive and respiratory syndrome virus (PRRSV) were identified. RT-PCR, Northern blot and sequence analyses indicated that these DI RNA specie have deletions of 8513-9176 nucleotides located between Nsp1/Nsp2 and Nsp10. Compared with the previous DI RNAs of PRRSV reported, they have three distinct characteristics: much smaller deletion sizes; different nucleotide repeats (2-12 nt) used in the junction sites and in-frame deletions. The results further suggested that the similarity-assisted RNA recombination may be the main cause of generation of DI RNAs in PRRSV and probably in other arteriviruses. (C) 2011 Elsevier B.V. All rights reserved.
摘要：
Transmissible proventriculitis associated with infectious bronchitis virus (IBV) was at first seen in eastern China in mid-1995, and is now endemic in China. Herein, the complete genome sequence of a proventiculitis-associated infectious bronchitis coronavirus (ZJ971) was sequenced and analyzed. Compared with the genome of the vaccine strain H120, ZJ971 had 54 nucleotide substitutions and a deletion in the 3'-UTR. The substitutions were in the regions of nsp2-nsp5, nsp7, nsp12, nsp13, nsp15, S and N genes, and the untranslating region. The results indicated that ZJ971 could be a variant of IBV strain H120.
摘要：
The objective of this study was to explore the immunomodulatory effects of betulinic acid (BA) extracted from the bark of white birch on mice. Female mice were orally administered BA for 14 days in doses of 0, 0.25, 0.5, and 1 mg/kg body weight. We found that BA significantly enhanced the thymus and spleen indices, and stimulated lymphocyte proliferation induced by Concanavalin A and lipopolysaccharide as shown by MTT assay. Flow cytometry revealed that BA increased the percentage of CD4(+) cells in thymus as well as the percentage of CD19(+) and the ratios of CD4(+)/CD8(+) in spleen. BA increased the number of plaque-forming cell and macrophage phagocytic activity as indicated by a neutral red dye uptake assay, and the peritoneal macrophages levels of TNF-alpha were also increased. In contrast, serum levels of IgG and IgM and serum concentrations of IL-2 and IL-6 were significantly decreased in BA-treated mice compared to the control as assayed by haemagglutination tests and ELISA, respectively. Taken together, these results suggest that BA enhances mouse cellular immunity, humoral immunity, and activity of macrophages. Thus, BA is a potential immune stimulator and may strengthen the immune response of its host.
作者机构：
[Zeng, D. L.; Liu, W.; Wang, K. Z.; Liu, Y.] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Zhu, X. Q.] CAAS, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Key Lab Vet Parasitol Gansu Prov, Lanzhou 730046, Gansu, Peoples R China.;[Zhao, G. H.] NW A&F Univ, Coll Vet Med, Yangling 712100, Shaanxi Prov, Peoples R China.;[Tan, M. Y.] Ctr Qual Quarantine Livestock Poultry & Aquacultu, Changsha 410006, Hunan, Peoples R China.;[Zhao, G. H.; Lin, R. Q.; Zhu, X. Q.; Yuan, Z. G.] S China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China.
通讯机构：
[Zhu, X. Q.] C;CAAS, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Key Lab Vet Parasitol Gansu Prov, Lanzhou 730046, Gansu, Peoples R China.
摘要：
The prevalence of spargana infection in frogs (Rana nigromaculata) was investigated in China's central Hunan Province, from March 2007 to October 2009. 59 of 292 (20.2%) wild-caught frogs were found to be infected with plerocercoids (spargana) of the genus Spirometra. Spargana were recovered from the skeletal muscle of the hind limb. The infection rate ranged from 4.5% to 27.4%, and the infection intensity was 1-15 spargana per frog. To identify the species identity of the collected spargana, a portion of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene was amplified, sequenced, and analyzed. Sequence variations for cox1 among all the examined spargana were 0.0-3.1%, with 14 variable sites being identified in sequences obtained (3.1%, 14/446), representing 6 different cox1 sequences. Phylogenetic analysis showed that all the spargana isolates in Hunan province represented Spirometra erinaceieuropaei. This is the first report of S. erinaceieuropaei infection in frogs in Hunan province, China. (C) 2010 Elsevier B.V. All rights reserved.
摘要：
Penicillic acid is one of the main mycotoxins in moldy feedstuff and has toxic effect on livestock and poultry and probably humans due to food chain transmission. The objective of this study was to generate and characterize a monoclonal antibody to penicillic acid for the efficient detection of penicillic acid from Penicillium cyclopiumby immunological methods. To this end, penicillic acid was conjugated to bovine serum album (BSA) using the Mannich reaction and coupled with ovalbumin (OVA) by the method of 1-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide hydrochloride (EDC) to generate artificial antigens penicillic acid-BSA and penicillic acid-OVA. A hybridoma cell line was obtained after fusion of mouse SP2/0 myeloma cells with spleen cells of BALB/c mice immunized with artificial antigen penicillic acid-BSA. A monoclonal antibody specific against penicillic acid was produced in vivo by this hybridoma cell line. Further analysis revealed that the monoclonal antibody to penicillic acid was of the IgG1 subtype, with a titer of 1: 2.05 × 105. The antibody to penicillic acid had no or less cross-reaction with mycotoxins, including aflatoxin B1, zearalenone, T-2 toxin and fumonisins and more importantly, it assumed an affinity of about 1.54 × 108 liters per mol. Our ability to produce a monoclonal antibody to penicillic acid provides necessary groundwork for the effective detection of penicillic acid in various tissues of animals and human, using the immunocytochemistry, Western blots and enzyme-linked immunosorbent assay (ELISA).
Abbreviation
EDC, 1-Ethyl-3-(3-dimethyl-aminopropyl) carbodiimide hydrochloride; BSA, bovine serum albumin; OVA, ovalbumin; PEG, polyethylene glycol; FCS, fetal calf serum; PBS, phosphate buffer saline; UV, ultraviolet; ELISA,enzyme-linked immunosorbent assay; HRP, horseradish peroxidase; HT,hypoxanthine-thymidine; HAT, hypoxanthine, aminopterin, and thymidine; DMF,dimethylformamide; NHS, N-hydroxysuccinimide; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis.
作者机构：
[Wang, Guiping; Yu, Xinglong; Hu, Shifeng] Hunan Agr Univ, Fac Vet, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Yu, Xinglong] H;Hunan Agr Univ, Fac Vet, Changsha 410128, Hunan, Peoples R China.
关键词：
avian influenza virus;latex agglutination test;matrix 1 protein
摘要：
The matrix 1 (M1) gene, present in all subtypes of avian influenza virus (AIV), was cloned and expressed in Escherichia coli. Reactivity of the expressed protein was confirmed by western blot. Subsequently, the M1 gene expression product was purified and used as the antigen to develop a latex agglutination test (LAT) for detecting antibodies against these conventional subtypes of AIV including AIV H3, H5, H7, and H9 from chicken sera. The LAT is specific for AIV, and no cross-reaction was shown with chicken antisera against other avian viruses. Compared with the hemagglutination inhibition test, the corresponding specificity, sensitivity, and correlation were 95.7%, 88.7%, and 89.0%, respectively, in detecting 491 serum samples from vaccinated chickens.
作者机构：
[Gong, Wenjie; Fan, Jinhong; Tu, Changchun; Shao, Mingfu; Jiang, Yu] Acad Mil Med Sci, Inst Vet Sci, Changchun 130062, Peoples R China.;[Gong, Wenjie] Jilin Univ, Inst Zoonoses, Changchun 130062, Peoples R China.;[Sun, Yanwei; Za, Yunfeng] Guangdong Ctr Anim Dis Control, Guangzhou 510230, Guangdong, Peoples R China.;[Xiong, Zhonglian; Zeng, Zheng] Chongqing Anim Dis Prevent & Control Ctr, Chongqing 401147, Peoples R China.;[Yu, Xinglong] Hunan Agr Univ, Vet Coll, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Tu, Changchun] A;Acad Mil Med Sci, Inst Vet Sci, 1068 Qinglong Rd, Changchun 130062, Peoples R China.
关键词：
China;Evolutionary dynamics;Rabies virus
摘要：
Phylogenetic studies have revealed a profound understanding about the biodiversity of rabies viruses in China, but little is known about their evolutionary dynamics in the country. In the present study, the complete G gene sequences of 33 rabies virus isolates (RABVs) isolated from distinct Chinese provinces were determined and phylogenetic analysis was conducted using these G sequences and 93 others retrieved from GenBank representing China and Southeast Asia. Further evolutionary history of RABV was estimated using a Bayesian Markov chain Monte Carlo method to understand the temporal and spatial dynamics of this virus. Results showed that rabies viruses in China and Southeast Asia share a common ancestor and form 2 clades with each being further divided into 3 lineages. The time of the most recent common ancestor of current RABV strains was estimated to be year 1654 (1514-1812) and the viruses circulating in Southeast Asia likely derived from China. (C) 2010 Elsevier B.V. All rights reserved.
摘要：
Very little information is known about the toxic effects of cadmium on somatic cells in mammalian testis. The objective of this study is to explore the toxicity of cadmium on piglet Sertoli cells. Sertoli cells were isolated from piglet testes using a two-step enzyme digestion and followed by differential plating. Piglet Sertoli cells were identified by oil red O staining and Fas ligand (FasL) expression as assayed by immunocytochemistry and expression of transferrin and androgen binding protein by RT-PCR. Sertoli cells were cultured in DMEM/F12 supplemented with 10% fetal calf serum in the absence or presence of various concentrations of cadmium chloride, or treatment with p38 MAPK inhibitor SB202190 and with cadmium chloride exposure. Apoptotic cells in seminiferous tubules of piglets were also performed using TUNEL assay in vivo. Cadmium chloride inhibited the proliferation of Piglet Sertoli cells as shown by MTT assay, and it increased malondialdehyde (MDA) but reduced superoxide dismutase (SOD) and Glutathione peroxidase (GSH-Px) activity. Inhibitor SB202190 alleviated the proliferation inhibition of cadmium on piglet Sertoli cells. Comet assay revealed that cadmium chloride caused DNA damage of Piglet Sertoli cells and resulted in cell apoptosis as assayed by flow cytometry. The in vivo study confirmed that cadmium induced cell apoptosis in seminiferous tubules of piglets. Transmission electronic microscopy showed abnormal and apoptotic ultrastructure in Piglet Sertoli cells treated with cadmium chloride compared to the control. cadmium has obvious adverse effects on the proliferation of piglet Sertoli cells and causes their DNA damage, cell apoptosis, and aberrant morphology. This study thus offers novel insights into the toxicology of cadmium on male reproduction.
摘要：
Preimplantation development is critical for successful implantation and pregnancy. In the mouse preimplantation embryo, the first event of morphological and cellular differentiation is established during polarization and compaction at the 8-cell stage. The considerable cell surface and cytoplasmic changes and formation of different populations of cells at the 8-cell stage are fundamentally important for the development of all organisms. To determine genes that are specifically expressed at this crucial stage of embryo development and also to shed light on the different mechanisms that could be of importance during embryo development, we investigated mouse 8-cell and 4-cell embryo stage-specific genes using Digital Differential Display (DDD). The 8-cell stage-specific genes were sorted according to their ontology data from the Database for Annotation, Visualization and Integrated Discovery (DAVID), which outlines possible roles for the genes expressed at the 8-cell stage. This study highlights how online tools can be used to identify genes involved in embryo development. Identification of the 8-cell embryo stage-specific genes would open new opportunities for understanding molecular networks during the mid-preimplantation gene activation. Using bioinformatic tools, such as Digital Differential Display and DAVID, it will be possible to identify genes expressed at the 8-cell stage that are likely to be involved in mammalian preimplantation embryo development. Our results may provide a new foundation for molecular control at the onset of embryonic development in mammals, and should be of interest to the scientific community.
摘要：
Xiao, H., JunFang, Lu, X., Liu, Z., Chen, X., Liu, J., Su, J., Yi, J. and Sun, Z. 2009. Protective effect of soluble fiber from Undaria pinnatifida on vascular endothelium in hypercholesterolemic rabbits. Can. J. Anim. Sci. 89: 361-367. Dietary fiber could improve endothelial function and abnormal production of nitric oxide (NO) and elevated endothelin-1 (ET-1) concentration induce endothelial dysfunction. In the present study, we tested the effect of soluble fiber extracted from Undaria pinnatifida (UP) on endothelial function and NO and ET-1 production in hypercholesterolemic rabbits. After treatment with UP soluble fiber (5 or 10%) for 8 wk, endothelium-dependent vasorelaxation in isolated aortic rings, the concentrations of NO and ET-1 and malondialdehyde (MDA), and the expression of endothelial NO synthase (eNOS) gene were measured. The UP soluble fiber (5 or 10%) treatment significantly attenuated the impairment of endotheliumdependent vasorelaxation concomitantly with increase of plasma NO concentration and expression of aortic endothelial nitric oxide synthase (eNOS), reduction of plasma MDA level and ET-1 concentration and aortic ET-1 concentration. The present study indicates that the protective effects of UP soluble fiber on endothelium-mediated vasorelaxation may be related to an improved NO production and a reduced ET-1 concentration in hypercholesterolemic rabbits.
作者机构：
[Liu, G. H.; Li, F.; Liu, W.; Liu, Y.; Dai, R. S.] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Lin, R. Q.; Zhu, X. Q.] S China Agr Univ, S China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China.;[Li, Z. Y.; Liu, D. X.] Gen Vet Serv Hunan Prov, Changsha 410007, Hunan, Peoples R China.;[He, S. W.] Xiangxi Nationality Vocat Tech Coll, Jishou 416000, Hunan, Peoples R China.;[Tan, M. Y.] Ctr Qual Quarantine Livestock Poultry & Aquacultu, Changsha 410006, Hunan, Peoples R China.
通讯机构：
[Zhu, X. Q.] S;S China Agr Univ, S China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China.
摘要：
The prevalence of helminths in adult dogs was investigated in Hunan Province, the People's Republic of China between June 2006 and December 2007. A total of 438 adult farm dogs slaughtered in local abattoirs from 9 representative administrative regions in Hunan Province were examined for the presence of helminths using a helminthological approach. All collected worms were counted and identified to species according to existing keys and descriptions. A total of 11 helminth species were found in the dogs, and they represented 2 phyla, 3 classes, 6 families and 8 genera. All dogs were infected by more than one helminth species. Clonorchis sinensis (29.4%) was the only trematode species found, Dipylidium caninum (42.3%) was the most common cestode species, and Toxocara canis (45.2%) the most common nematode species. 6 of the 11 dog helminths are also transmissible to humans (i.e., zoonotic), and can cause severe clinical human diseases, posing significant public health threats. The results of the present investigation have implications for the ongoing control of helminth infections in dogs and humans in Hunan Province, China.
作者：
Wang, G. P.;Be, Y. Y.;Hu, S. F.;Liu, W.;Liu, G. Y.*
期刊：
JOURNAL OF ANIMAL AND FEED SCIENCES,2009年18(1):61-70 ISSN：1230-1388
通讯作者：
Liu, G. Y.
作者机构：
[Hu, S. F.; Wang, G. P.; Liu, W.; Be, Y. Y.] Hunan Agr Univ, Fac Vet, Changsha 410128, Hunan, Peoples R China.;[Liu, G. Y.] Yunnan Agr Univ, Kell Lab Anim Nutr & Feed Yunnan Prov, Kunming 650201, Peoples R China.
通讯机构：
[Liu, G. Y.] Y;Yunnan Agr Univ, Kell Lab Anim Nutr & Feed Yunnan Prov, Kunming 650201, Peoples R China.
摘要：
The complete coding sequences of three porcine genes - NDP, CLN3 and NCF1 - were amplified using the reverse transcriptase polymerase chain reaction (RT-PCR) based on the conserved sequence information of the human, mouse, and referenced highly homologues pig ESTs. The sequence analyses of these three genes revealed that porcine NDP gene encodes a protein of 133 amino acids which has high homology with the Norrie disease protein homolog (NDP) of four species: bovine (98%) human (96%), crab-eating macaque (96%) and mouse (95%). The porcine CLN3 gene encodes a protein of 438 amino acids which has high homology with the ceroid-lipofuscinosis, neuronal 3 (CLN3) of four species: human (90%), crab-eating macaque (90%), dog (89%) and mouse (83%). The porcine NCF1 gene encodes a protein of 392 amino acids that has high homology with the neutrophil cytosolic factor 1(NCF1) of three species: bovine (87%), human (82%) and mouse (79%). Phylogenetic tree analysis revealed that the porcine NDP and NCF1 has closer genetic relationships with the bovine NDP and NCF1, but the porcine CLN3 has a closer genetic relationship with the CLN3 of human and crab-eating macaque. The tissue transcription profile analyses indicated that pig CLN3 and NCF1 genes were generally expressed in most of tissues, pig NDP gene was expressed in muscle, spleen, brain, lung but not expressed in kidney, liver, backfat and pancreas. These data serve as a foundation for further research on these three genes which have been associated with human disease.
摘要：
Previous investigations have indicated that reduced erythrocyte deformability may be an important factor contributing to the development of atherosclerosis, and endogenous asymmetric dimethylarginine (ADMA) might be an important contributor to reduction of erythrocyte deformability in atherosclerosis. In this study, the effect of 3,4,5,6-tetrahydroxyxanthone (1), a kind of polyphenolic compound, on erythrocyte deformability in apolipoprotein E-deficient (apoE − / − ) mice was evaluated. After treatment with compound 1 (10 or 20 mg/kg per day) for 4 weeks, erythrocyte deformability, antioxidant enzymes activity, erythrocyte dimethylarginine dimethylaminohydrolase (DDAH) activity, the plasma level of ADMA and malondialdehyde (MDA) level were determined. Treatment with compound 1 (10 or 20 mg/kg) increased erythrocyte deformability, antioxidant enzymes activity concomitantly, a decrease in the plasma levels of MDA and ADMA, and an increase in erythrocyte DDAH activity. The present result suggests that the beneficial effect of 1 on the erythrocyte deformability, besides inhibiting lipid peroxidation, may be related to reduction of ADMA concentration via an increase in DDAH activity.
作者机构：
[Song, H. Q.; Zhao, G. H.; Weng, Y. B.; Mo, X. H.; Li, J.; Lin, R. Q.; Yuan, Z. G.; Zhu, X. Q.] S China Agr Univ, Coll Vet Med, Parasitol Lab, Guangzhou 510642, Guangdong, Peoples R China.;[Zou, F. C.] Yunnan Agr Univ, Coll Anim Sci & Technol, Kunming 650201, Yunnan Province, Peoples R China.;[Liu, W.] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Zhu, X. Q.] S China Agr Univ, Coll Vet Med, Parasitol Lab, 483 Wushan St, Guangzhou 510642, Guangdong, Peoples R China.
通讯机构：
[Zhu, X. Q.] S;S China Agr Univ, Coll Vet Med, Parasitol Lab, 483 Wushan St, Guangzhou 510642, Guangdong, Peoples R China.
关键词：
Major Histocompatibility Complex;Schistosoma;Major Histocompatibility Complex Gene;Schistosoma Japonicum;Unweighted Pair Group Method With Arithmetic Average
