作者：
Liu, Z. -Y.;Dai, M. -H.;Tao, Y. -F.;Chen, D. -M.;Yuan, Z. -H.*
期刊：
Journal of Veterinary Pharmacology and Therapeutics,2011年34(5):424-429 ISSN：0140-7783
通讯作者：
Yuan, Z. -H.
作者机构：
[Liu, Z. -Y.] Huazhong Agr Univ, Coll Vet Med, MAO Key Lab Food Safety Evaluat, Natl Reference Lab Vet Drug Residues HZAU, Wuhan 430070, Hubei, Peoples R China.;[Liu, Z. -Y.] Hunan Agr Univ, Fac Vet, Changsha, Hunan, Peoples R China.;[Yuan, Z. -H.] Huazhong Agr Univ, Coll Vet Med, MAO Key Lab Food Safety Evaluat, Natl Reference Lab Vet Drug Residues HZAU, Shizishan St, Wuhan 430070, Hubei, Peoples R China.
通讯机构：
[Yuan, Z. -H.] H;Huazhong Agr Univ, Coll Vet Med, MAO Key Lab Food Safety Evaluat, Natl Reference Lab Vet Drug Residues HZAU, Shizishan St, Wuhan 430070, Hubei, Peoples R China.
摘要：
Liu, Z.‐Y., Dai, M.‐H., Tao, Y.‐F., Chen, D.‐M., Yuan, Z.‐H. Inhibition of cytochrome P450 2A participating in coumarin 7‐hydroxylation in pig liver microsomes. J. vet. Pharmacol. Therap. 34, 424–429. Five commonly used human cytochrome P450 (CYP) inhibitors were examined for their effects on coumarin 7‐hydroxylase (CYP2A) activity in pig liver microsomes. The K m and V max values for coumarin 7‐hydroxylation in pig liver microsomes were estimated to be 1 μm and 0.26 nmol·mg/min, respectively. The following human CYP inhibitors caused little or no inhibition of CYP2A as defined by a K i > 200 μm: quinidine (CYP2D6), troleandomycin (CYP3A4), and sulfaphenazole (CYP2C9). The other two human CYP inhibitors were classified as strong inhibitors of CYP2A: 8‐methoxypsoralen (CYP2A6) and α‐naphthoflavone (CYP1A1/2). In the absence of a preincubation period, 8‐MOP inhibited the 7‐hydroxylation of coumarin with a K i value of 1.1 μm, which decreased to 0.1 μm when 8‐MOP was preincubated with pig liver microsomes for 3 min. α‐Naphthoflavone inhibited the 7‐hydroxylation of coumarin with a K i value of 32 μm, which did not increase ability to inhibitor CYP2A when α‐naphthoflavone was preincubated with pig liver microsomes for 3 min. These results of this study suggest that 8‐MOP is a potent, mechanism‐based inhibitor of pig CYP2A activity in pig liver microsomes.
摘要：
Olaquindox is a growth-promoting feed additive for food-producing animals. Its toxicities were reported to be closely related to the metabolism. To provide the interpretation of toxicities in animals, this study explored the metabolism of olaquindox in rats, chickens and pigs of different genders by qualitative metabolite profiling. Animals were fed olaquindox in an oral dose, and then their urine, plasma, feces, liver, kidney and muscle were collected. Liquid chromatography combined with hybrid ion trap/time-of-flight mass spectrometry was used for structural investigation and identification of metabolites. The structures of metabolites were elucidated based on the accurate MS2 spectra and comparison of their changes in accurate molecular masses and fragment ions with those of parent drug or metabolite. A total of 18, 18 and 16 metabolites of rats, chickens and pigs were identified, respectively. Among the identified metabolites, 8 known metabolites were confirmed as an early study had stated, and 15 metabolites were found for the first time in vivo. The major metabolic pathways of olaquindox were proposed to be N-O reduction and oxidation of hydroxyl to carboxylic acid followed by N-O reduction. The qualitative species difference on the metabolite profiles of olaquindox among the three species was observed. However, metabolite profiles of olaquindox appeared to be qualitatively similar between female and male for the same species. The proposed metabolic pathways of olaquindox in animals will provide comprehensive data to clarify the metabolism of olaquindox among different species, and will give scientific explanation for toxicities and residues of olaquindox. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
作者机构：
[Qiu, Li-Ling; Lin, Rui-Qing; Liu, Guo-Hua; Zhu, Xing-Quan] CASS, Dept Parasitol, State Key Lab Vet Etiol Biol, Lanzhou Vet Res Inst,Key Lab Vet Parasitol Gansu, Lanzhou 730046, Gansu, Peoples R China.;[Yuan, Zi-Guo; Qiu, Li-Ling; Lin, Rui-Qing; Pan, Hong; Weng, Ya-Biao; Hou, Jie] S China Agr Univ, Parasitol Lab, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China.;[Liu, Guo-Hua] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Wu, Xiang-Yun] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China.;[Xie, Wen-Qin] Yongzhou Vocat Tech Coll, Dept Agr Sci & Technol, Yongzhou 425000, Hunan, Peoples R China.
通讯机构：
[Liu, Guo-Hua] C;CASS, Dept Parasitol, State Key Lab Vet Etiol Biol, Lanzhou Vet Res Inst,Key Lab Vet Parasitol Gansu, Lanzhou 730046, Gansu, Peoples R China.
摘要：
In recent years, surveys of Toxoplasma gondii infection in dogs have been reported worldwide, including China. However, little is known about the prevalence of T. gondii in pet dogs in Northwest China. In the present study, the prevalence of T. gondii in pet dogs in Lanzhou, China was investigated using the modified agglutination test (MAT). In this survey, antibodies to T. gondii were found in 28 of 259 (10.81%) pet dogs, with MAT titers of 1:20 in 14 dogs, 1:40 in nine, 1:80 in four, and 1:160 or higher in one dog. The prevalence ranged from 6.67% to 16.67% among dogs of different ages, with low rates in young pet dogs, and high rates in older pet dogs. The seroprevalence in dogs >3 years old was higher than that in dogs ≤1 years old, but the difference was not statistically significant (P > 0.05). The seroprevalence in male dogs was 12.50% (17 of 136), and in female dogs it was 8.94% (11 of 123), but the difference was not statistically significant (P > 0.05). A high prevalence of T. gondii infection was found in pet dogs in Lanzhou, Northwest China, which has implications for public health in this region. In order to reduce the risk of exposure to T. gondii, further measures and essential control strategies should be carried out rationally in this region.
摘要：
Bisdesoxyolaquindox is a reduced metabolite of olaquindox which is used as a medicinal feed additive in veterinary medicine. The relevant metabolism studies of bisdesoxyolaquindox have been carried out for the first time in rat, chicken, and pig liver subcellular fractions in order to understand the metabolic enzymes that are possibly responsible for the metabolism of olaquindox. The metabolites were characterized by high-performance liquid chromatography combined with hybrid ion trap/time-of-flight mass spectrometry. The major metabolic pathways of bisdesoxyolaquindox in the three species were the oxidation of hydroxyl to bisdesoxyolaquindox-2'-carboxyl acid (O10) and the N-dealkylation of the side chain to 3-methylquinoxaline 2-carboxamide (O12). Other metabolic pathways were also proposed which involved the direct methyl oxidation and N-oxide on the quinoxaline ring in the three species as well as N-hydroxylation only in rat. The intrinsic clearance values in the liver microsomes for O10 and O12 were ranked in the order of chicken > pig >> rat and rat > pig >> chicken, respectively. Inhibition studies indicated that 8-methoxypsoralen, 4-methylpyrazole and alpha-naphthoflavone could inhibit the formations of O10 and O12 in all species. Quinidine, troleandomycin, diethyldithiocarbamate, and disulfiram showed an interspecies difference in the inhibition of the formation of two metabolites. In rat and pig liver cytosol, 4-methylpyrazole, menadione and chlorpromazine strongly inhibited the formation of O10. Both diethyldithiocarbamate and disulfiram were found to inhibit O10 formation in rat cytosol but not in pig cytosol. These results indicated the following: In rat liver microsomes. CYP2A might be involved in the formation of O10, and CYP1A, CYP2A and CYP2E would be involved in the O12 formation. In pig liver microsomes, CYP1A and CYP2E might catalyze the formations of O10 and O12. In rat cytosol, alcohol dehydrogenase, aldehyde oxidase and aldehyde dehydrogenase should catalyze the O10 formation. In pig cytosol, alcohol dehydrogenase and aldehyde oxidase might be involved in the formation of O10. In chicken, it was found that various CYP isoenzymes were capable of catalyzing the two reactions: none of the inhibitors of cytosol enzymes inhibited O10 formation in chicken cytosol. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
通讯机构：
[Xue, Li-Qun] H;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
关键词：
Mouse model;PCV-2b;Histopathological lesions;Viral distribution
摘要：
The viral distribution and lesions in Kunming mice experimentally infected with porcine circovirus type 2b (PCV-2b) were investigated. Seventy special pathogen free mice were divided into 2 groups with 35 mice in each group. The test group (TG) was infected with PCV-2b, the control group (CG) was inoculated with sterile cell cultures. Five mice in each group were sacrificed at 3, 7, 14, 21, 28, 35 and 42 dpi (day post infection), respectively. Necropsies were performed on all mice and tissues were collected for testing by histopathology, immunohistochemistry, transmission electron microscope (TEM) and polymerase chain reaction (PCR). Apoptosis and necrosis in lymphoid organs were observed in virus-infected mice, and became severe from 14 to 28 dpi. The proportion of PCV-2b antigen-positive cells was moderate in lung, heart, thymus, liver or kidney, and low in brain from TG. In spleen and cervical lymph node, the proportions of PCV-2b antigen-positive cells were low to high from 7 to 28 dpi, and moderate from 35 to 42 dpi. PCV-2b DNA was detected in all tissues examined in TG from 7 to 42 dpi. Viral inclusion bodies presented in the cytoplasm of lymphocytes, macrophages, hepatocytes, podocytes, neurocytes, spermatids and uterine epithelial cells in TG. In CG, no viruses and viral lesions were detected. PCV-2b could replicate in mice, and PCV-2b associated lesions in mice were similar to those observed in pigs. The present results indicate that it is possible to use Kunming mouse as an animal model for PMWS research.
摘要：
Mequindox (MEQ) is a novel synthetic quinoxaline 1,4-dioxides antibacterial agent and growth promoter in animal husbandry. This study was to investigate whether reactive oxygen species (ROS), the Janus kinase-signal transducer and activator of transcription (JAK/STAT) pathway, suppressors of cytokine signaling (SOCS) and inflammatory cytokines were involved in toxicities of MEQ Our data demonstrated that high dose of MEQ (275 mg/kg) apparently led to tissue impairment combined with imbalance of redox in liver. In liver and spleen samples, hydroxylation metabolites and desoxymequindox were detected, directly confirming the potential link of N -> O group reduction metabolism with its organ toxicity. Moreover, up-regulation of JAK/STAT, SOCS family, tumor necrosis factor (TNF-alpha) and interleukin-6 (IL-6) were also observed in the high-dose group. Meanwhile, significant changes of oxidative stress indices in liver were observed in the high-dose group. As for NADPH subunit, the mRNA levels of many subunits were significantly up-regulated at low doses but down-regulated in a dose-dependent manner in liver and spleen, suggesting an involvement of NADPH in MEQ metabolism and ROS generation. In conclusion, we reported the dose-dependent long-term toxicity as well as the discussion of the potential mechanism and pathways of MEQ which raised further awareness of its toxicity following with the dose change. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
摘要：
Conjugates of the Escherichia coli serum antibody (Ab) with the gentamicin sulfate (GM sulfate) have been evaluated for the assessment of their ability to eradicate E. coli in vitro. The combination of every component in the targeted drug is the amino of serum antibody and gentamicin sulfate combined with the hydroxyl of PEG6000 by hydrogen bond. which forms stable complexes. The half-life of this complex is 4.83 h, and it is non-toxic side effects and low immunogenic. After the combination of antibody and gentamicin sulfate, the targeting of antibody is quite good. In vitro anti-bacterial activity of gentamicin sulfate increased 1000 times, and the clinical curative effect enhanced 100 times, this means higher efficacy and safety. (C) 2011 Elsevier Ltd. All rights reserved.
摘要：
Objective: To investigate the reproductive toxicity and cytotoxicity of T-2 toxin, which is a mycotoxin, and to explore its potential apoptotic induction mechanism. Methods: Ovarian granulosa cells of rats were treated with T-2 toxin (1-100 nM) for 24 h. The cytotoxicity was assessed with MTT bioassay; apoptotic cells were identified microscopically by chromatin condensation and nuclear fragmentation with Hoechst 33258; mitochondrial membrane potential with hodamine 123 and reactive reactive oxygen species (ROS) with 2',7'-dichlorofluoresceinacetate (DCFH-DA) was analyzed by fluorometry; p53 and other apoptosis-related proteins such as Bax, Bcl-2, caspase-3, caspase-9 were determined by Western blot analysis, and related mRNA expressions were determined by reverse transcriptase-polymerase chain reaction (RT-PCR). The caspase activity was measured by cleavage of the caspase substrate (Ac-DEVD-pNA for caspase-3. Ac-LEHD- pNA for caspase-9). Results: T-2 toxin inhibited the growth of granulosa cells in a concentration-dependent way. The result of Hoechst 33258 staining indicated that T-2 toxin induces granulosa cells apoptosis based on the typical apoptotic morphological changes. Subsequently, we found that T-2 toxin treatment induced ROS accumulation in granulosa cells, resulting in reduction of mitochondrial transmembrane potential. The induction of cell apoptosis was caused by the upregulation of p53, Bax, Bcl-2, Bax/Bcl-2 ration, and the activation of the caspases pathways. T-2 toxin-induced apoptotic granulosa cells significantly decreased through the use of antioxidant Trolox. Conclusion: These data suggest a possible underlying molecular mechanism for T-2 toxin that induces the apoptosis signaling pathway in rat granulosa cells by regulation of ROS-mediated mitochondrial pathway. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
作者机构：
[Song, Hui-Qun; Huang, Si-Yang; Lin, Rui-Qing; Liu, Guo-Hua; Zhu, Xing-Quan; Tan, Hong-Wei] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Lanzhou, Gansu, Peoples R China.;[Liu, Guo-Hua; Zhu, Xing-Quan] Hunan Agr Univ, Coll Vet Med, Changsha, Hunan, Peoples R China.;[Dong, Xia; Tan, Hong-Wei] Yunnan Agr Univ, Eastern Bee Res Inst, Kunming, Yunnan Prov, Peoples R China.;[Yuan, Zi-Guo; Lin, Rui-Qing] S China Agr Univ, Coll Vet Med, Guangzhou, Guangdong, Peoples R China.;[Zhao, Guang-Hui] NW A&F Univ, Coll Vet Med, Yangling, Shaanxi Prov, Peoples R China.
通讯机构：
[Tan, Hong-Wei] C;Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Lanzhou, Gansu, Peoples R China.
关键词：
Transfer RNA;Genomics;Invertebrate genomics;Hymenoptera;Honey bees;Ribosomal RNA;Mitochondria;Mitochondrial DNA
摘要：
In the present study, we determined the complete mitochondrial DNA (mtDNA) sequence of Apis cerana, the Asiatic cavity-nesting honeybee. We present here an analysis of features of its gene content and genome organization in comparison with Apis mellifera to assess the variation within the genus Apis and among main groups of Hymenoptera. The size of the entire mt genome of A. cerana is 15,895 bp, containing 2 ribosomal RNA genes, 13 protein-coding genes, 22 transfer RNA (tRNA) genes and one control region. These genes are transcribed from both strands and have a nucleotide composition high in A and T. The contents of A+T of the complete genomes are 83.96% for A. cerana. The AT bias had a significant effect on both the codon usage pattern and amino acid composition of proteins. There are a total of 3672 codons in all 13 protein-coding genes, excluding termination codons. The most frequently used amino acid is Leu (15.52%), followed by Ile (12.85%), Phe (10.10%), Ser (9.15%) and Met (8.96%). Intergenic regions in the mt genome of A. cerana are 705 bp in total. The order and orientation of the gene arrangement pattern is identical to that of A. mellifera, except for the position of the tRNA-Ser(AGN) gene. Phylogenetic analyses using concatenated amino acid sequences of 13 protein-coding genes, with three different computational algorithms (NJ, MP and ML), all revealed two distinct groups with high statistical support, indicating that A. cerana and A. mellifera are two separate species, consistent with results of previous morphological and molecular studies. The complete mtDNA sequence of A. cerana provides additional genetic markers for studying population genetics, systematics and phylogeographics of honeybees.
摘要：
The prevalence of Enterococcus faecalis was investigated in diseased swine in Hunan Province, China between February 2007 and February 2011. A total of 385 diseased swine dissected in prosectorium from 10 representative administrative regions in Hunan Province were examined for the presence of E. faecalis. Forty-two out of 385 (10.91%) diseased swine were found to be infected with E. faecalis by bacteria isolations. E. faecalis was mainly recovered from the lung and joint cavity. Identification of the organisms was based on biochemical assays and sequencing of the partial 16S rRNA. The results of the present investigation have implications for the ongoing control of E. faecalis infections in swine and humans in Hunan Province, China.
通讯机构：
[Yuan, Hui] H;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
关键词：
DNA damage;Gossypol;Oxidase;Sertoli cells
摘要：
The study was designated to explore the toxic effects of gossypol on piglet sertoli cells. Sertoli cellswere isolated from piglet testes using a two-step enzyme digestion and followed by differential plating.Piglet sertoli cells were cultured and classified into five groups, that is, group A, the control withoutgossypol, group B with 2.5 μg/ml gossypol, group C with 5 μg/ml gossypol, group D with 10 μg/mlgossypol and group E with 20 μg/ml gossypol. We found that sertoli cells’ growth was inhibited bygossypol at dose 2.5 μg/ml when compared with the control group. The oxidase activity of sertoli cellalso decreased at 2.5 μg/m gossypol. Moreover, DNA damage of sertoli cells was observed at 5 μg/mlgossypol. Putting this into consideration, our study suggests that exposure of gossypol to sertoli cellsleads to an inhibition of sertoli cell growth and oxidase activity of sertoli cells at a low concentration,whereas gossypol results in DNA damage of sertoli cells at a higher concentration.Keywords: Gossypol, sertoli cells, oxidase, DNA damage
摘要：
The study was designed to explore the toxic effects of arsanilic acid on piglet Sertoli cells. Sertoli cells were isolated from piglet testes using a two-step enzyme digestion followed by differential plating. Piglet Sertoli cells were cultured and classified into the following five groups: group A, the control without arsanilic acid treatment; group B, cultured with 5 mu M arsanilic acid; group C, cultured with 50 mu M arsanilic acid; group D, cultured with 0.5 mM arsanilic acid; and group E, cultured with 5 mM arsanilic acid. We found that Sertoli cell growth was inhibited by arsanilic acid at 0.5 mM compared with the control, group A. The oxidase activity of Sertoli cells was decreased by arsanilic acid at 0.5 mM as evidenced by the observations that arsanilic acid increased MDA content but decreased the SOD and GSH-Px activities of Sertoli cells. Moreover, 50 mu M of arsanilic acid was observed to cause DNA damage in Sertoli cells. The results of our study suggest that exposure of Sertoli cells to arsanilic acid leads to induction of oxidative stress and inhibition of cell growth at a high concentration, while arsanilic acid causes DNA damage in Sertoli cells at a low concentration.
期刊：
Rapid Communications in Mass Spectrometry,2011年25(2):341-348 ISSN：0951-4198
通讯作者：
Yuan, Zong-Hui
作者机构：
[Chen, Dong-Mei; Wang, Xu; Liu, Zhao-Ying; Yuan, Zong-Hui; Tao, Yan-Fei] Huazhong Agr Univ, Coll Vet Med, HZAU MAO Key Lab Food Safety Evaluat, Natl Reference Lab Vet Drug Residues, Wuhan 430070, Hubei, Peoples R China.;[Liu, Zhao-Ying] Hunan Agr Univ, Fac Vet, Changsha 410128, Hunan, Peoples R China.;[Yuan, Zong-Hui] Huazhong Agr Univ, Coll Vet Med, HZAU MAO Key Lab Food Safety Evaluat, Natl Reference Lab Vet Drug Residues, Shizishan St, Wuhan 430070, Hubei, Peoples R China.
通讯机构：
[Yuan, Zong-Hui] H;Huazhong Agr Univ, Coll Vet Med, HZAU MAO Key Lab Food Safety Evaluat, Natl Reference Lab Vet Drug Residues, Shizishan St, Wuhan 430070, Hubei, Peoples R China.
摘要：
Carbadox (methyl-3-(2-quinoxalinylmethylene)-carbazate-N-1, N-4-dioxide) is a chemotherapeutic growth promoter added to feed for starter pigs. In this work, the metabolism of carbadox in rat, pig and chicken liver microsomes has been studied firstly. The incubation mixtures were then processed and analyzed for metabolites with a sensitive and reliable method based on high-performance liquid chromatography combined with hybrid ion trap/time-of-flight mass spectrometry (LC/MS-IT-TOF). With the help of chromatographic behavior and accurate mass measurements, it is possible to rapidly and reliably characterize the metabolites of carbadox. The structural elucidations of these metabolites were performed by comparing the changes in the accurate molecular masses and fragment ions generated from precursor ions with those of parent drug. The present results showed that the metabolism of carbadox in liver microsomes had qualitative species-difference. A total of seven metabolites were identified in rat liver microsomes. Five metabolites (Cb1-Cb3, Cb5, Cb7) were observed in pig and chicken liver microsomes. In addition, metabolite Cb6 was also detected in chicken liver microsomes. The peak areas of the metabolites in the three species are different. For the formations of Cb1, Cb2, Cb5 and Cb6, the rank order was rat>chicken>pig; Cb3; pig similar to chicken>rat. Cb1, Cb2 and Cb3 have been previously reported, whereas the other four metabolites were novel. The N -> O group reduction and hydroxylation followed by N -> O group reduction were the main metabolic pathways for carbadox in the three species. Copyright (C) 2010 John Wiley & Sons, Ltd.
作者机构：
[Yin, Jiechao; Li, Jing; Li, Guangxing; Ren, Xiaofeng] NE Agr Univ, Coll Vet Med, Harbin 150030, Peoples R China.;[Yin, Jiechao] NE Agr Univ, Coll Life Sci, Harbin 150030, Peoples R China.;[Yang, Qing] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Ren, Xiaofeng] NE Agr Univ, Coll Vet Med, 59 Mucai St, Harbin 150030, Peoples R China.
通讯机构：
[Ren, Xiaofeng] N;NE Agr Univ, Coll Vet Med, 59 Mucai St, Harbin 150030, Peoples R China.
摘要：
Avian infectious bronchitis virus (IBV), a coronavirus, causes infectious bronchitis leading to enormous economic loss in the poultry industry worldwide. Houttuynia cordata (Saururaceae) (HC) is a traditional Chinese medicine used in China. In the present study, the effect of HC on cell infection by IBV was determined using plaque assay and reverse transcription-polymerase chain reaction. The inhibitory effect of HC on IBV infection in ovo and in vivo was analysed using specific pathogen free (SPF) chicken embryos and chickens. Moreover, the effect of HC on cell apoptosis induced by IBV was investigated. Results showed that HC had more than 90% inhibition rate against IBV infection in Vero cells and chicken embryo kidney cells, and decreased more than 90% apoptotic cells caused by IBV. HC fully protected the SPF embryos, and had more than 50% protection rate in SPF chickens, against IBV challenge.
作者：
Lin, R. Q.;Wang, X. Q.;Yan, C.;He, X. H.;Cheng, T.;...
期刊：
African Journal of Microbiology Research,2011年5(24):4240-4242 ISSN：1996-0808
通讯作者：
Zhang, Y. B.
作者机构：
[Yan, C.; Wang, X. Q.; Lin, R. Q.; Xu, M. J.; Zhu, X. Q.] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Lanzhou 730046, Gansu, Peoples R China.;[Zhang, Y. B.] Sun Yat Sen Univ, Affiliated Hosp 3, Dept Emergency Med, Guangzhou 510630, Guangdong, Peoples R China.;[Wang, Y. N.; Wang, X. Q.; Lin, R. Q.; Cheng, T.; He, X. H.; Yuan, Z. G.] S China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China.;[Yan, C.] Xuzhou Med Coll, Dept Pathogen Biol & Immunol, Xuzhou 221004, Jiangsu, Peoples R China.;[Zhu, X. Q.] Heilongjiang Bayi Agr Univ, Coll Anim Sci & Vet Med, Daqing 163319, Heilongjiang Pr, Peoples R China.
通讯机构：
[Zhang, Y. B.] S;Sun Yat Sen Univ, Affiliated Hosp 3, Dept Emergency Med, Guangzhou 510630, Guangdong, Peoples R China.
关键词：
Chlamydophila psittaci is widely distributed in a number of birds and humans throughout the world;but little is known of the prevalence of C. psittaci in birds in China. In the present study;sera from 240 chickens;200 ducks;200 geese and 200 pigeons in China’s southern Guangdong Province were assayed for Chlamydophila antibodies by indirect hemagglutination assay (IHA). The specific antibodies were found in sera of 17.92% of 240 chickens;6% of 200 ducks;22% of 200 geese and 17% of 200 pigeons. Statistical analysis showed that the seroprevalence of C. psittaci in ducks was significantly lower than that in chickens;geese and pigeons;respectively (P < 0.05). The seroprevalence in free-range chickens was 10.83%;and it was 25% in caged chickens;and the difference was statistically significant (P < 0.05). Among different age groups of pigeons;the difference in seroprevalence of C. psittaci between adult pigeons and adolescent pigeons was significant (P < 0.05). This is the first time that the seroprevalence of Chlamydophila infection in domestic birds in Southern China was reported;and the data showed that the prevalence of Chlamydophila infection in domestic birds is high;indicating that the domestic birds may be an important source for human infection by C. psittaci in Southern China.
摘要：
Chlamydophila psittaci is widely distributed in a number of birds and humans throughout the world, but little is known of the prevalence of C. psittaci in birds in China. In the present study, sera from 240 chickens, 200 ducks, 200 geese and 200 pigeons in China's southern Guangdong Province were assayed for Chlamydophila antibodies by indirect hemagglutination assay (IHA). The specific antibodies were found in sera of 17.92% of 240 chickens, 6% of 200 ducks, 22% of 200 geese and 17% of 200 pigeons. Statistical analysis showed that the seroprevalence of C. psittaci in ducks was significantly lower than that in chickens, geese and pigeons, respectively (P < 0.05). The seroprevalence in free-range chickens was 10.83%, and it was 25% in caged chickens, and the difference was statistically significant (P < 0.05). Among different age groups of pigeons, the difference in seroprevalence of C. psittaci between adult pigeons and adolescent pigeons was significant (P < 0.05). This is the first time that the seroprevalence of Chlamydophila infection in domestic birds in Southern China was reported, and the data showed that the prevalence of Chlamydophila infection in domestic birds is high, indicating that the domestic birds may be an important source for human infection by C. psittaci in Southern China.
期刊：
Toxicology and Applied Pharmacology,2011年252(3):281-288 ISSN：0041-008X
通讯作者：
Yuan, Zonghui
作者机构：
[Wang, Yulian; Li, Tingting; Wang, Xu; Liu, Zhaoying; Liu, Yu; Ihsan, Awais; Yu, Huan; Yuan, Zonghui; Zhang, Hongfei; Yang, Chunhui; Huang, Xianju] Huazhong Agr Univ, Natl Reference Lab Vet Drug Residues, Wuhan 430070, Peoples R China.;[Yuan, Zonghui] Huazhong Agr Univ, Natl Reference Lab Vet Drug Residues HZAU, MOA Key Lab Food Safety Evaluat, Wuhan 430070, Peoples R China.;[Liu, Zhaoying] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Huang, Xianju] S Cent Univ Nationalities, Coll Pharm, Wuhan 430074, Peoples R China.
通讯机构：
[Yuan, Zonghui] H;Huazhong Agr Univ, Natl Reference Lab Vet Drug Residues HZAU, MOA Key Lab Food Safety Evaluat, Wuhan 430070, Peoples R China.
关键词：
DNA damage;Mequindox;Oxidative stress;Quinoxaline;Rats;Testis;Testosterone;8-hydroxydeoxyguanosine (8-OHdG)
摘要：
Mequindox (MEQ) is a synthetic antimicrobial chemical of quinoxaline 1, 4-dioxide group. This study was designed to investigate the hypothesis that MEQ exerts testicular toxicity by causing oxidative stress and steroidal gene expression profiles and determine mechanism of MEQ testicular toxicity. In this study, adult male Wistar rats were fed with MEQ for 180 days at five different doses as 0, 25, 55, 110 and 275 mg/kg, respectively. In comparison to control, superoxide dismutase (SOD), reduced glutathione (GSH) and 8-hydroxydeoxyguanosine (8-OHdG) levels were elevated at 110 and 275 mg/kg MEQ whereas the malondialdehyde (MDA) level was slightly increase at only 275 mg/kg. Furthermore, in LC/MS-IT-TOF analysis, one metabolite 2-isoethanol 4-desoxymequindox (M11) was found in the testis. There was significant decrease in body weight, testicular weight and testosterone at 275 mg/kg, serum follicular stimulating hormone (FSH) at 110 and 275 mg/kg, while lutinizing hormone (LH) levels were elevated at 110 mg/kg. Moreover, histopathology of testis exhibited germ cell depletion, contraction of seminiferous tubules and disorganization of the tubular contents of testis. Compared with control, mRNA expression of StAR, P450scc and 17 beta-HSD in testis was significantly decreased after exposure of 275 mg/kg MEQ while AR and 3 beta-HSD mRNA expression were significantly elevated at the 110 mg/kg MEQ group. Taken together, our findings provide the first and direct evidence in vivo for the formation of free radicals during the MEQ metabolism through N -> O group reduction, which may have implications to understand the possible mechanism of male infertility related to quinoxaline derivatives. (C) 2011 Elsevier Inc. All rights reserved.
