通讯机构：
[Zhixing Qing; Fenglian Luo] A;Authors to whom correspondence should be addressed.<&wdkj&>Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China
关键词：
genus Zingiber;phytochemistry;pharmacology;ethnomedicine;gingerols;Zingiberaceae
摘要：
Plant of the genus Zingiber (Zingiberaceae) have primarily distributed in subtropical and tropical Asia, South America and Africa. The species of this genus have been widely used as food and in folk with a long history for treating various diseases. Reports related to the phytochemistry and phytochemistry of Zingiber species are numerous, but articles on the summary of the genus Zingiber remain scarce. This review aims at presenting comprehensive information about the genus Zingiber and providing a reference for the future application by systematically reviewing the literature from 1981 to 2020. Currently, a total of 447 phytochemical constituents have been isolated and identified from this genus, in which volatile oils, diarylheptanoids, gingerols, flavonoids and terpenoids are the major components. Gingerols, which are the main functional components, are the spicy and aromatic ingredients in the Zingiber species. Extracts and single compounds from Zingiber plants have been discovered to possess numerous biological functions, such as anti-inflammatory, anticancer, antimicrobial, larvicidal, antioxidant and hypoglycemic activities. This review provides new insights into the ethnomedicine, phytochemistry and pharmacology of the genus Zingiber and brings to the forefront key findings on the functional components of this genus in food and pharmaceutical industries.
通讯机构：
[Jine Yi; Lijuan Zhu] H;Hunan Engineering Research Center of Livestock and Poultry Health Care, College of Veterinary Medicine, Hunan Agricultural University, Changsha, China
作者：
Sun, L. L.;Liu, L.;Brenna, J. T.;Wu, Z. H.;Ma, L.;...
期刊：
Animal,2022年16(6):100545 ISSN：1751-7311
通讯作者：
Bu, D.P.
作者机构：
[Wu, Z. H.; Bu, D. P.; Sun, L. L.] Chinese Acad Agr Sci, Inst Anim Sci, State Key Lab Anim Nutr, Beijing 100193, Peoples R China.;[Liu, L.] Hunan Agr Univ, Coll Vet Med, Changsha 410000, Peoples R China.;[Brenna, J. T.] Univ Texas Austin, Dell Pediat Res Inst, Dept Nutr & Chem, Austin, TX 78705 USA.;[Wu, Z. H.] Chinese Acad Agr Sci CAAS, Joint Lab Integrated Crop Tree Livestock Syst, Ethiopian Inst Agr Res EIAR, Beijing 100193, Peoples R China.;[Bu, D. P.] Chinese Acad Agr Sci CAAS, Joint Lab Integrated Crop Tree Livestock Syst, World Agroforestry Ctr ICRAF, Beijing 100193, Peoples R China.
通讯机构：
[D.P. Bu] S;State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China<&wdkj&>Joint Laboratory on Integrated Crop-Tree-Livestock Systems of the Chinese Academy of Agricultural Sciences (CAAS), Ethiopian Institute of Agricultural Research (EIAR) and World Agroforestry Centre (ICRAF), Beijing 100193, China<&wdkj&>Hunan Co-Innovation Center of Safety Animal Production, Changsha 410000, China
期刊：
Journal of Electroanalytical Chemistry,2022年911:116186 ISSN：1572-6657
通讯作者：
Qin, XL;Gu, CY
作者机构：
[Xu, Mingyang; Wang, Birui; Yang, Xiaolan; Qin, Xiaoli; Xu, Wenjing; Li, Xiangyu; Zhou, Yuqing] Hunan Agr Univ, Coll Chem & Mat Sci, Changsha 410128, Peoples R China.;[Ding, Yanbin] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Peoples R China.;[Gu, Chaoyue] Capital Normal Univ, Dept Chem, Beijing 100048, Peoples R China.
通讯机构：
[Gu, CY ] C;[Qin, XL ] H;Hunan Agr Univ, Coll Chem & Mat Sci, Changsha 410128, Peoples R China.;Capital Normal Univ, Dept Chem, Beijing 100048, Peoples R China.
关键词：
Electrochemical detection;Immunosensor;Metal organic frameworks;One-step synthesis;Toluidine blue
摘要：
Toluidine blue (TB) as an electrochemically active molecule usually loads on metal organic frameworks (MOFs) surface to improve its electrochemical performance. In this paper, a simple synthesis strategy for one-step fabricating toluidine blue (TB)-functionalized metal organic frameworks (TB@MOFs) via the self-assembly approach was reported. This reaction of trimesic acid (H3BTC) as an organic ligand, TB as an electrochemical probe and a base for the deprotonation of H3BTC, and Zn2+, Cu2+ or Co2+ as the metal ion source leads to a rapid synthesis of three different MOFs, which all show exceptional electrochemical activity due to abundant TB molecules encapsulated in the MOFs. Varied instruments were used to characterize the TB@MOFs to verify the successful synthesis. Based on these three TB@MOFs sensing platforms, the sensitive label-free electrochemical immunosensors for the detection of pig immunoglobulin G (IgG), mycobacterium bovis (MB) and foot and mouth disease virus (FMDV) were developed with wide linear ranges and low detection limits. Furthermore, these immunosensors present good sensitivity, selectivity and stability in actual samples.
期刊：
FRONTIERS IN ONCOLOGY,2022年12:994672 ISSN：2234-943X
通讯作者：
Duan, Y.;Liu, W.
作者机构：
[Gong, Tengfang; Liu, Wei; Ou, Xidan; Chen, Shuyu] Hunan Agr Univ, Coll Vet Med, Res Ctr Parasites & Vectors, Changsha, Peoples R China.;[Zheng, Jie; Yu, Jiayi; Zheng, Changbing; Duan, Yehui] Chinese Acad Sci, Inst Subtrop Agr, CAS Key Lab Agroecol Proc Subtrop Reg, Hunan Prov Key Lab Anim Nutr Physiol & Metab Proc,, Changsha, Peoples R China.;[Zheng, Jie; Yu, Jiayi; Duan, Yehui] Univ Chinese Acad Sci, Coll Adv Agr Sci, Beijing, Peoples R China.
通讯机构：
[Liu, W.] R;[Duan, Y.] C;CAS Key Laboratory of Agro-ecological Processes in Subtropical Region, China;Research Center for Parasites & Vectors, China
关键词：
cancer cells;Gln metabolism;health;oxidative homeostasis;ROS
通讯机构：
[Zhihang Yuan; Jine Yi] H;Hunan Engineering Research Center of Livestock and Poultry Health Care, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China
作者：
Kim, Hani Jieun;Li, Pishun;Kim, Taiyun;Oldfield, Andrew J.;Zheng, Xiaofeng;...
期刊：
iScience,2022年25(10):105049 ISSN：2589-0042
通讯作者：
Xiaofeng Zheng<&wdkj&>Pengyi Yang
作者机构：
[Yang, Pengyi; Kim, Taiyun; Kim, Hani Jieun] Childrens Med Res Inst, Computat Syst Biol Grp, Westmead, NSW 2145, Australia.;[Li, Pishun; Zheng, Xiaofeng] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Peoples R China.;[Yang, Pengyi; Kim, Taiyun; Kim, Hani Jieun] Univ Sydney, Sch Math & Stat, Sydney, NSW 2006, Australia.;[Yang, Pengyi] Univ Sydney, Charles Perkins Ctr, Sydney, NSW 2006, Australia.;[Oldfield, Andrew J.] Univ Montpellier, Inst Genet Humaine, CNRS UMR9002, F-34000 Montpellier, France.
通讯机构：
[Xiaofeng Zheng; Pengyi Yang] C;Computational Systems Biology Group, Children’s Medical Research Institute, Westmead, NSW 2145, Australia<&wdkj&>School of Mathematics and Statistics, University of Sydney, Sydney, NSW 2006, Australia<&wdkj&>Charles Perkins Centre, University of Sydney, Sydney, NSW 2006, Australia<&wdkj&>College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China
关键词：
Molecular biology;molecular mechanism of gene regulation;omics
摘要：
Lysine-specific demethylase 1 (LSD1) is well-known for its role in decommissioning enhancers during mouse embryonic stem cell (ESC) differentiation. Its role in gene promoters remains poorly understood despite its widespread presence at these sites. Here, we report that LSD1 promotes RNA polymerase II (RNAPII) pausing, a rate-limiting step in transcription regulation, in ESCs. We found the knockdown of LSD1 preferentially affects genes with higher RNAPII pausing. Next, we demonstrate that the co-localization sites of LSD1 and MYC, a factor known to regulate pause-release, are enriched for other RNAPII pausing factors. We show that LSD1 and MYC directly interact and MYC recruitment to genes co-regulated with LSD1 is dependent on LSD1 but not vice versa. The co-regulated gene set is significantly enriched for housekeeping processes and depleted of transcription factors compared to those bound by LSD1 alone. Collectively, our integrative analysis reveals a pleiotropic role of LSD1 in promoting RNAPII pausing.
摘要：
Gamma-oryzanol (Orz), a mixture of the ferulic acid ester of triterpene alcohols and phytosterols, was found abundantly in rice bran and rice bran oil which could be available and served as an antioxidant. The present study was to explore the potential protective effects of Orz on oxidative stress and cell apoptosis in human hepatic cells (L02 cells) induced by hydrogen peroxide (H(2) O(2) ). Flow cytometry detection and Hoechst 33258 staining showed that Orz significantly restored cell cycle and ameliorated apoptosis in H(2) O(2) -challenged L02 cells. Orz pretreatment inhibited H(2) O(2) -induced cell apoptosis by increasing the scavenging of hydroxyl radicals (OH·), and efficiently decreasing the production of nitric oxide (NO). Moreover, a loss of total antioxidant capacity (T-AOC) and adenosine triphosphatase (ATPase) were enhanced in H(2) O(2) -mediated L02 cells pretreated with Orz. Furthermore, preincubation with Orz reduced H(2) O(2) -mediated the proapoptotic protein of Bak expression and the phosphorylation of ASK1, p38, JNK, and ERK, and increased the anti-apoptotic protein of Bcl-xl expression and anti-oxidative stress proteins of Nrf2 and HO-1 expression. The findings suggested that Orz exerts the cytoprotective effects in H(2) O(2) -induced L02 cells apoptosis by ameliorating oxidative stress via inhibiting MAPK signaling pathway and activating Nrf2 signaling pathway. PRACTICAL APPLICATIONS: Gamma-oryzanol (Orz), a mixture of the ferulic acid ester of triterpene alcohols and phytosterols, was found abundantly in rice bran and rice bran oil which could be availably served as an antioxidant. In this study, it was found that Orz exerts the cytoprotective effects in H(2) O(2) -induced L02 cell apoptosis by ameliorating oxidative stress via the inhibition of MAPK signaling pathway and the activation of Nrf2 signaling pathway, which provides a theoretical basis for dietary adding natural products to prevent or treat oxidative stress-related diseases.
期刊：
International Journal of Molecular Sciences,2022年23(21):13092- ISSN：1422-0067
通讯作者：
Guo-Hua Liu
作者机构：
[Fu, Yi-Tian; Liu, Guo-Hua; Wang, Hui-Mei] Hunan Agr Univ, Coll Vet Med, Res Ctr Parasites & Vectors, Changsha 410128, Peoples R China.;[Suleman] Univ Swabi, Dept Zool, Swabi 23561, Pakistan.;[Yao, Chaoqun] Ross Univ, Sch Vet Med, Dept Biomed Sci, POB 334, Basseterre, St Kitts & Nevi.;[Yao, Chaoqun] Ross Univ, One Hlth Ctr Zoonoses & Trop Vet Med, Sch Vet Med, POB 334, Basseterre, St Kitts & Nevi.;[Wang, Wei] Univ Sunshine Coast, Ctr Bioinnovat, Sch Sci & Engn, Sippy Downs, Qld 4556, Australia.
通讯机构：
[Guo-Hua Liu] R;Research Center for Parasites & Vectors, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Author to whom correspondence should be addressed.
摘要：
Sucking lice are obligate ectoparasites of mammalian hosts, causing serious public health problems and economic losses worldwide. It is well known that sucking lice have fragmented mitochondrial (mt) genomes, but many remain undetermined. To better understand patterns of mt genome fragmentation in the sucking lice, we sequenced the mt genome of the buffalo louse Haematopinus tuberculatus using next-generation sequencing (NGS). The mt genome of H. tuberculatus has ten circular minichromosomes containing a total of 37 genes. Each minichromosome is 2.9-5.0 kb long and carries one to eight genes plus one large non-coding region. The number of mt minichromosomes of H. tuberculatus (ten) is different from those of congeneric species (horse louse H. asini, domestic pig louse H. suis and wild pig louse H. apri) and other sucking lice. Two events (gene translocation and merger of mt minichromosome) are observed in Haematopinus. Compared to other studies, our phylogeny generated from mt genome datasets showed a different topology, suggesting that inclusion of data other than mt genomes would be required to resolve phylogeny of sucking lice. To our knowledge, this is the first report of a ten mt minichromosomes genome in sucking lice, which opens a new outlook into unexplored mt genome fragmentation patterns in sucking lice.
摘要：
Cucurbitacin C-type cucurbitacins that are only identified in Cucumis sativus (cucumber) are, in part, responsible for the health benefits and bitter flavor. Nevertheless, detailed information about those functional ingredients in cucumber is scarce. In this study, ten cucurbitacin C analogues including seven undescribed ones have been isolated from the bitter leaves of cucumber, in which six compounds showed growth inhibition capabilities against tumor cell lines HepG2, A549, DU145 and HCT116. Intriguingly, cucurbitacin C6 and C7 exhibited a significant inhibition effect compared to the positive control taxol (IC50 = 1.86 +/- 0.17 mu M) on HepG2 cell line with IC50 values of 10.06 +/- 0.34 mu M and 4.16 +/- 0.42 mu M, respectively. The mechanism of cucurbitacin-induced apoptosis is likely down-regulating the expression of caspase-related proteins. This work enlarges the knowledge of the cucurbitacins in cucumber and highlights the importance of cucumber as a source of specialized metabolites in the food and medicinal industries.
期刊：
FRONTIERS IN PLANT SCIENCE,2022年13:1051756 ISSN：1664-462X
通讯作者：
Su, Y.;Xiao, L.-T.
作者机构：
[Wu, Zi-Han; Su, Yi; Wang, Ruo-Zhong; Xiao, Lang-Tao] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Peoples R China.;[Sun, Zhi-Liang] Hunan Agr Univ, Coll Vet Med, Changsha, Peoples R China.
通讯机构：
[Su, Y.; Xiao, L.-T.] C;College of Bioscience and Biotechnology, China
关键词：
DESI-MSI;Gelsemium elegans;alkaloid;mass spectrometry imaging;spatio-temporal distribution
摘要：
Gelsemium elegans contains multiple alkaloids with pharmacological effects, thus researchers focus on the identification and application of alkaloids extracted from G. elegans. Regretfully, the spatiotemporal distribution of alkaloids in G. elegans is still unclear. In this study, the desorption electrospray ionization mass spectrometry imaging (DESI-MSI) was applied to simultaneously analyze the distribution of pharmacologically important alkaloids in different organ/tissue sections of G. elegans at different growth stages. Finally, 23 alkaloids were visualized in roots, stems and leaves at seedling stage and 19 alkaloids were observed at mature stage. In mature G. elegans, 16 alkaloids were distributed in vascular bundle region of mature roots, 15 alkaloids were mainly located in the pith region of mature stems and 2 alkaloids were enriched in epidermis region of mature stems. A total of 16 alkaloids were detected in leaf veins of mature leaves and 17 alkaloids were detected in shoots. Interestingly, diffusion and transfer of multiple alkaloids in tissues have been observed along with the development and maturation. This study comprehensively characterized the spatial metabolomics of G. elegans alkaloids, and the spatiotemporal distribution of alkaloid synthesis. In addition, the results also have reference value for the development and application of Gelsemium elegans and other medicinal plants.
摘要：
Excessive inflammation in bovine mammary endothelial cells (BMEC) due to mastitis leads to disease progression and eventual culling of cattle. Sirtuin 3 (SIRT3), a mitochondrial deacetylase, downregulates pro-inflammatory cytokines in BMEC exposed to high concentrations of nonesterified fatty acids by blunting nuclear factor-¦ÊB (NF¦ÊB) signaling. In nonruminants, SIRT3 is under the control of PGC1¦Á, a transcriptional cofactor. Specific aims were to study (1) the effect of SIRT3 on inflammatory responses of lipopolysaccharide (LPS)-challenged bovine mammary epithelial cells (bovine mammary alveolar cells-T, MAC-T) models, and (2) the role of PGC1¦Á in the attenuation of NF¦ÊB signaling via SIRT3. To address these objectives, first, MAC-T cells were incubated in triplicate with 0, 50, 100, 150, or 200 ¦Ìg/mL LPS (derived from Escherichia coli O55:B5) for 12 h with or without a 2-h incubation of the NF¦ÊB inhibitor ammonium pyrrolidine dithiocarbamate (APDC, 10 ¦ÌM). Second, SIRT3 was overexpressed using adenoviral expression (Ad-SIRT3) at different multiplicity of infection (MOI) for 6 h followed by a 12 h incubation with 150 ¦Ìg/mL LPS. Third, cells were treated with the PGC1¦Á agonist ZLN005 (10 ¦Ìg/mL) for 24 h and then challenged with 150 ¦Ìg/mL LPS for 12 h. Fourth, cells were initially treated with the PGC1¦Á inhibitor SR-18292 (100 ¦ÌM) for 6 h followed by a 6-h culture with or without 50 MOI Ad-SIRT3 and a challenge with 150 ¦Ìg/mL LPS for 12 h. Data were analyzed using one-way ANOVA with subsequent Bonferroni correction. Linear and quadratic contrasts were used to determine dose-responses to LPS. There were linear and quadratic effects of LPS dosage on cell viability. Incubation with 150 and 200 ¦Ìg/mL LPS for 12 h decreased cell viability to 78.6 and 34.9%, respectively. Compared with controls, expression of IL1B, IL6, and TNFA was upregulated by 5.2-, 5.9-, and 2.7-fold with 150 ¦Ìg/mL LPS; concentrations of IL-1¦Â, IL-6, and tumor necrosis factor-¦Á (TNF-¦Á) in cell medium also increased. Compared with the LPS group, LPS+APDC increased cell viability and reversed the upregulation of IL1B, IL6, and TNFA expression. However, mRNA and protein abundance of SIRT3 decreased linearly with increasing LPS dose. Ad-SIRT3 infection (50 MOI) reduced IL1B, IL6, and TNFA expression and also their concentrations in cell medium, and decreased pNF¦ÊB P65/NF¦ÊB P65 ratio and nuclear abundance of NF¦ÊB P65. The PGC1¦Á agonist increased SIRT3 expression, whereas it decreased cytokine expression, pNF¦ÊB P65/NF¦ÊB P65 ratio, and prevented NF¦ÊB P65 nuclear translocation. Contrary to the agonist, the PGC1¦Á inhibitor had opposite effects, and elevated the concentrations of IL-1¦Â, IL-6, and TNF-¦Á in cell medium. Overall, data suggested that SIRT3 activity could attenuate LPS-induced inflammatory responses in mammary cells via alterations in the PGC1¦Á-NF¦ÊB pathway. As such, there may be potential benefits for targeting SIRT3 in vivo to help prevent or alleviate negative effects of mastitis.
通讯机构：
[Yulong Yin; Daojun Xu] A;Authors to whom correspondence should be addressed.<&wdkj&>College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China
关键词：
black soldier fly;trommel screener;differential separation;design;test
通讯机构：
[Zhao-Ying Liu; Zhao-Ying Liu Zhao-Ying Liu Zhao-Ying Liu] C;College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan, China<&wdkj&>Hunan Engineering Technology Research Center of Veterinary Drugs, Hunan Agricultural University, Changsha, Hunan, China
作者机构：
[Fang, Manxin; Hu, Wei] Yichun Univ, Coll Life Sci & Resource Environm, Yichun 336000, Jiangxi, Peoples R China.;[Deng, Zhibang] Res Ctr Reverse Vaccinol RCRV, Prov Key Lab Prot Engn Anim Vaccines, Changsha 410128, Hunan, Peoples R China.;[Deng, Zhibang] Hunan Agr Univ, Coll Vet Med, Lab Funct Prote LFP, Changsha 410128, Hunan, Peoples R China.;[Deng, Zhibang] Hunan Agr Univ, Res Ctr Reverse Vaccinol RCRV, Prov Key Lab Prot Engn Anim Vaccines, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Deng, Zhibang] Hunan Agr Univ, Coll Vet Med, Lab Funct Prote LFP, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Zhibang Deng *] P;Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Research Center of Reverse Vaccinology (RCRV), and Laboratory of Functional Proteomics (LFP), Hunan Agricultural University College of Veterinary Medicine, Changsha, China
摘要：
Background: Swine viruses are well known as a threat to the pig industry. Many signaling pathways and a number of proteins were discovered to participate in the immune responses to swine viruses. Noncoding RNAs (ncRNAs), comprising a different set of transcripts including housekeeping RNAs (for example, rRNAs and tRNAs) and regulatory RNAs (small RNAs and long ncRNAs), recently have been described as important regulators of viral infections regarding swine. Summary: A growing number of studies have shown ncRNAs are an indispensable part in response to virus infection, involved not only in antiviral responses, but in many interactions between host and virus, some of which may benefit the certain types of swine virus. Key Messages: Here, we review the relationship of ncRNA and viruses through several representative swine viruses. Further, we discuss the potential of using ncRNAs as antiviral biomarkers.
