期刊：
npj Biofilms and Microbiomes,2023年9(1):62 ISSN：2055-5008
通讯作者：
Xiong, Xia;Yin, YL;Xiong, X;Yin, Jie
作者机构：
[Zhou, Xihong; He, Yiwen; Peng, Can; Yin, Yulong; Xiong, Xia; Liang, Jing; Yin, YL] Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha, Peoples R China.;[Zhou, Xihong; Yin, Yulong; Xiong, Xia; Liang, Jing; Yin, YL] Univ Chinese Acad Sci, Coll Adv Agr Sci, Beijing, Peoples R China.;[Zhou, Xihong; Xiong, Xia; Huang, Chunxia] Changsha Med Univ, Sch Stomatol, Changsha, Peoples R China.;[He, Yiwen] Hunan Normal Univ, Coll Life Sci, Hunan Prov Key Lab Anim Intestinal Funct & Regulat, Changsha, Peoples R China.;[Chen, Jingqing] Acad Mil Med Sci, Lab Anim Ctr, Beijing, Peoples R China.
通讯机构：
[Xiong, X; Xiong, X ; Yin, YL ] C;[Yin, J] H;Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha, Peoples R China.;Univ Chinese Acad Sci, Coll Adv Agr Sci, Beijing, Peoples R China.;Changsha Med Univ, Sch Stomatol, Changsha, Peoples R China.
摘要：
Diarrhea is characterized by alterations in the gut microbiota, metabolites, and host response to these changes. Studies have focused on the role of commensal bacteria in diarrhea; however, the effect of fungi on its pathogenesis remains unexplored. Here, using post-weaned piglets with or without diarrhea, we found an unexpected decrease in the abundance of Candida tropicalis in diarrheal piglets. We also observed increased accumulation of reactive oxygen species (ROS) and the formation of neutrophil extracellular traps (NETs) in the colonic tissues of diarrheal piglets. Using dectin-1-knockout mice, we found that the over-accumulation of ROS killed C. tropicalis by promoting NET formation, which was dependent on dectin-1. The decreased abundance of C. tropicalis resulted in reduced phosphocholine consumption. Then, colonic phosphocholine accumulation drives water efflux by increasing cAMP levels by activating adenylyl cyclase, which promotes the clearance of pathogenic bacteria. Collectively, we demonstrated that phosphocholine is correlated with colonic C. tropicalis and promotes diarrhea and pathogen clearance. Our results suggest that mycobiota colonizing the colon might be involved in maintaining intestinal metabolic homeostasis through the consumption of certain metabolites.
摘要：
Ornithine α-ketoglutarate (OKG), a nutritional compound, is an amino acid salt with anti-oxidative and anti-inflammatory effects on humans and animals. Ulcerative colitis (UC), as an inflammatory bowel disease (IBD), leads to chronic intestinal inflammatory dysfunction. This study evaluated the optimal dosage of OKG in healthy mice. Then, a mouse model of acute colitis was established using dextran sodium sulfate (DSS), and the preventive effect of OKG on DSS-induced colitis in mice was explored through analysis of serum inflammatory cytokines and fecal microbiota. Initially, the mice were randomly divided into a control group, a group given a low dose of OKG (LOKG: 0.5%), a group given a medium dose of OKG (MOKG: 1%), and a group given a high dose of OKG (HOKG: 1.5%); they remained in these groups for the entire 14-day experimental period. Our results demonstrated that 1% OKG supplementation increased body weight, serum growth hormone (GH), insulin (INS), alkaline phosphatase (ALP), Tyr, and His and decreased urea nitrogen (BUN), NH(3)L, and Ile. Then, a 2 × 2 factor design was used for a total of 40 mice, with diet (a standard diet or a 1% OKG diet) and challenge (4% DSS or not) as the main factors. During days 14 to 21, the DSS mice were administered 4% DSS to induce colitis. The results revealed that OKG alleviated weight loss and reversed the increases in colonic histological damage induced by DSS. OKG also increased serum IL-10 secretion. Moreover, OKG enhanced the abundance of Firmicutes and decreased that of Bacteriodetes at the phylum level and particularly enhanced the abundance of Alistipes and reduced that of Parabacterioides at the genus level. Our results indicated that OKG promotes growth performance and hormone secretion and regulates serum biochemical indicators and amino acid concentrations. Furthermore, 1% OKG supplementation prevents DSS-induced colitis in mice via altering microbial compositions and reducing the secretion of inflammatory cytokines in serum.
摘要：
Dietary nutrients and the gut microbiota are increasingly recognized to cross-regulate and entrain each other, and thus affect host health and immune-mediated diseases. Here, we systematically review the current understanding linking dietary nutrients to gut microbiota-host immune interactions, emphasizing how this axis might influence host immunity in health and diseases. Of relevance, we highlight that the implications of gut microbiota-targeted dietary intervention could be harnessed in orchestrating a spectrum of immune-associated diseases.
摘要：
This study aimed to investigate the impact of compound organic acid (COA) and chlortetracycline (CTC) on serum biochemical parameters, intestinal health, and growth performance of weaned piglets. Twenty-four piglets (24 d of age) were randomly allocated into three treatments with eight replicate pens (one piglet per pen). Feed the basal diet or a diet containing 3,000 mg/kg COA or 75 mg/kg CTC, respectively. Results showed that both COA and CTC significantly increased average daily gain and reduced diarrhea rates (P < 0.05). They also upregulated serum total antioxidant capacity and downregulated serum interleukin (IL-10) levels (P < 0.05), increased crude protein digestibility and propionic acid concentration in the colon, and decreased spermidine and putrescine contents (P < 0.05). Intestinal microbiota analysis revealed that both COA and CTC increased the Shannon and Chao1 index and decreased the relative abundance of Blautia and Roseburia, but increased the relative abundance of Clostridium-sensu-stricto-1. Correlation analysis indicated that Clostridium-sensu-stricto-1 may be closely related to inflammation levels and microbial metabolites in piglets. Based on the results, COA may be a potential substitute for CTC to reduce antibiotic use and biogenic amine emission while improving piglet growth and intestinal health. Lay Summary Weaned piglets face challenges due to their underdeveloped digestive system, resulting in high gastrointestinal tract pH and insufficient enzyme secretion. To address this issue, we found supplementing piglet diets with 3,000 mg/kg of compound organic acid positively impacted the immune and antioxidant levels of piglets, promoted their intestinal health, improved nutrient digestibility, and enhanced their overall growth performance. These benefits were attributed to the regulation of intestinal microbiota by the compound organic acid. By improving piglet health and growth, this research offers a potential solution to the challenges of piglet weaning stress. Piglet weaning stress can significantly impact intestinal health and growth performance. However, supplementing piglet diets with compound organic acid can regulate microbiota, promote antioxidant capacity, enhance intestinal health, and improve nutrient digestibility. These positive effects contribute to better piglet growth performance, providing a promising solution to the challenges of piglet weaning stress.
摘要：
Muscle cell growth plays an important role in skeletal muscle development. Circular RNAs (circRNAs) have been proven to be involved in the regulation of skeletal muscle growth and development. In this study, we explored the effect of circTTN on myoblast growth and its possible molecular mechanism. Using C2C12 cells as a functional model, the authenticity of circTTN was confirmed by RNase R digestion and Sanger sequencing. Previous functional studies have showed that the overexpression of circTTN inhibits myoblast proliferation and differentiation. Mechanistically, circTTN recruits the PURB protein on the Titin (TTN) promoter to inhibit the expression of the TTN gene. Moreover, PURB inhibits myoblast proliferation and differentiation, which is consistent with circTTN function. In summary, our results indicate that circTTN inhibits the transcription and myogenesis of the host gene TTN by recruiting PURB proteins to form heterotypic complexes. This work may act as a reference for further research on the role of circRNA in skeletal muscle growth and development.
摘要：
In the genomes of diploid organisms, runs of homozygosity (ROH), consecutive segments of homozygosity, are extended. ROH can be applied to evaluate the inbreeding situation of individuals without pedigree data and to detect selective signatures via ROH islands. We sequenced and analyzed data derived from the whole-genome sequencing of 97 horses, investigated the distribution of genome-wide ROH patterns, and calculated ROH-based inbreeding coefficients for 16 representative horse varieties from around the world. Our findings indicated that both ancient and recent inbreeding occurrences had varying degrees of impact on various horse breeds. However, recent inbreeding events were uncommon, particularly among indigenous horse breeds. Consequently, the ROH-based genomic inbreeding coefficient could aid in monitoring the level of inbreeding. Using the Thoroughbred population as a case study, we discovered 24 ROH islands containing 72 candidate genes associated with artificial selection traits. We found that the candidate genes in Thoroughbreds were involved in neurotransmission (CHRNA6, PRKN, and GRM1), muscle development (ADAMTS15 and QKI), positive regulation of heart rate and heart contraction (HEY2 and TRDN), regulation of insulin secretion (CACNA1S, KCNMB2, and KCNMB3), and spermatogenesis (JAM3, PACRG, and SPATA6L). Our findings provide insight into horse breed characteristics and future breeding strategies.
摘要：
Abstract This experiment was conducted to investigate the effects of magnolol on the oxidative parameters and jejunum injury induced by diquat in broiler chickens. This test adopts a 2 × 2 factors design, a total of 288 one‐day‐old male AA broiler chicks randomly allocated to four groups, consisting of six replicates of 12 birds each, which was then denoted as CON group, diquat (DIQ) group (16 mg/kg BW diquat was injected into birds at the age of 21 days), magnolol (MAG) group (basic bird diet supplemented with 300 mg/kg magnolol), and MAG + DIQ group. At 21 days of age, broilers in the DIQ group and the MAG + DIQ group were intraperitoneally injected with 16 mg/kg BW diquat. Results showed that diet supplementing with MAG could alleviate the decrease of ADG to a certain extent after exposure to DIQ. Addition of magnolol to the diet alleviated the decrease of ADG during injection, antioxidant enzymes, and gene expression and increased the markers of oxidative damage induced by diquat induction. Magnolol supplement reversed the increase of apoptotic cells in the diquat‐induced chicken jejunum. RNA sequencing showed that PI3K–Akt, calcium, and NF‐kappa B signaling pathways were the main enrichment pathways between the DIQ group and the MAG + DIQ group. Our findings revealed that magnolol may improve antioxidant enzyme activity and expression of related genes through the PI3K–Akt pathway to alleviate oxidative stress.
