摘要：
The wolf spider Lycosa singoriensis is a hunting spider with a widespread distribution in northwest China. The venom gland of spiders, which is a vent specialized secretory tissue, can secrete abundant and complex toxin components. To extensively examine the transcripts expressed in the venom glands of L. singoriensis, we generated 833 expressed sequence tags (ESTs) from a directional cDNA library. Toxin-like sequences account for 69.1% of these ESTs, 17.3% are similar to cellular transcripts and 13.6% have no significant similarity to any known sequences. Here, we identified 223 novel toxin-like sequences, which can be classified into six different. superfamilies; that means a novel potential source of ligands for varied ion channels was discovered. With the aid of Gene Ontology terms and homology to eukaryotic orthologous groups, the annotation of cellular transcripts revealed some cellular processes important for the toxin secretion of venom glands including protein synthesis, protein folding, tuned post-translational processing and trafficking, etc. (C) 2009 Elsevier GmbH. All rights reserved.
摘要：
High levels of polyunsaturated fatty acids (PUFA) in pork are beneficial and saturated fatty acids (SFA) detrimental to human health. Inclusion of PUFA in diet may decrease the amount of intramuscular fat (IMF) due to inhibition of de nova synthesis of fatty acids, which has a negative effect on meat eating quality. Diet energy level is a leading factor for lipid deposition in IMF. We hypothesize that increasing dietary energy level by including PUFA will increase its content without decreasing IMF in meat, thus increasing meat's nutritional value without lowering meat eating quality. The results of this study showed that increasing dietary energy level by inclusion of soy oil decreased average daily feed intake (ADFI) (P<0.01) and feed:gain ratio (P<0.01), increased average daily weight gain ADG (P<0.05) and serum triglyceride concentration (P<0.05) and decreased serum urea concentration (P<0.05). Carcass characteristics, meat quality and serum concentrations of hormones [insulin, growth hormone (GH), insulin-like growth factor-I (IGF-I), glucagon and tumor necrosis factor-alpha (TNF-alpha)] were unaffected. Percentages of SFA and monounsaturated fatty acids (MUFA) were decreased while percentages of PUFA were increased (P<0.05) in longissimus dorsi (LD) muscle as dietary energy level increased. The results of this study suggested that increasing dietary energy level by inclusion of soy oil could be a suitable way to improve growth performance as well as nutritional value of pork without lowering eating quality.
摘要：
Xiao, H., JunFang, Lu, X., Liu, Z., Chen, X., Liu, J., Su, J., Yi, J. and Sun, Z. 2009. Protective effect of soluble fiber from Undaria pinnatifida on vascular endothelium in hypercholesterolemic rabbits. Can. J. Anim. Sci. 89: 361-367. Dietary fiber could improve endothelial function and abnormal production of nitric oxide (NO) and elevated endothelin-1 (ET-1) concentration induce endothelial dysfunction. In the present study, we tested the effect of soluble fiber extracted from Undaria pinnatifida (UP) on endothelial function and NO and ET-1 production in hypercholesterolemic rabbits. After treatment with UP soluble fiber (5 or 10%) for 8 wk, endothelium-dependent vasorelaxation in isolated aortic rings, the concentrations of NO and ET-1 and malondialdehyde (MDA), and the expression of endothelial NO synthase (eNOS) gene were measured. The UP soluble fiber (5 or 10%) treatment significantly attenuated the impairment of endotheliumdependent vasorelaxation concomitantly with increase of plasma NO concentration and expression of aortic endothelial nitric oxide synthase (eNOS), reduction of plasma MDA level and ET-1 concentration and aortic ET-1 concentration. The present study indicates that the protective effects of UP soluble fiber on endothelium-mediated vasorelaxation may be related to an improved NO production and a reduced ET-1 concentration in hypercholesterolemic rabbits.
作者：
LI Ai-gao;WANG Tao;YUAN You-lu;LIU Guang-ping;ZHANG Bao-cai;...
作者机构：
[LI Ai-gao; WANG Tao; YUAN You-lu; LIU Guang-ping; ZHANG Bao-cai; LI Jun-wen; SHI Yu-zhen; LIU Ai-ying; YANG Ze-mao] Cotton Research Institute,Chinese Academy of Agricultural Sciences,Key Laboratory of Cotton Genetic Improvement,Ministry of Agriculture,Anyang,Henan 455000,China;[LI Ai-gao; YU Xiao-nan] College of Agronomy,Hunan Agricultural University,Changsha,Hunan 410128,China;[LIU Guang-ping; YANG Ze-mao; LIU Zhi] College of Life Science and Technology,Hunan Agricultural University,Changsha,Hunan 410128,China
会议名称：
2008 ICGI Research Conference(2008年国际棉花基因组研究大会)
会议时间：
2008-7-8
会议地点：
河南安阳
会议主办单位：
中国棉花学会;中国遗传学会;国际棉花基因组学研究学会;中国农业科学院
会议论文集名称：
2008 ICGI Research Conference(2008年国际棉花基因组研究大会)论文集
摘要：
Shikonin and its derivatives are formed in large amounts in dark-cultured Onosma paniculatum cells. In order to isolate and identify the genes regulating shikonin biosynthesis, we constructed and characterized a full-length-enriched cDNA Library of dark-cultured cells by using the SMART (Switching Mechanism At 5'-end of RNA Transcript) cDNA synthesis and LD-PCR (tong-distance PCR) strategies. The titer of the primary cDNA library was 1.04 x 10(6) pfu/mL with a recombination rate of 99.60%. Most of the cDNA inserts ranged from 1.0 to 2.5 kb, and 78.33% of the 76 randomly selected clones contained full-length coding regions. Expression analysis of randomly selected genes by small scale microarray revealed that 23 genes were down-regulated, including 17 genes with known functions, 2 genes with putative functions, and 4 novel genes, and that 3 genes were up-regulated (two-fold) in cells cultured under white tight as compared with those cultured in the dark. Interestingly, two of the down-regulated genes, encoding aci-reductone dioxygenase (ARD)-like protein and ethylene responsive factor (ERF), are involved in ethylene biosynthesis and signal transduction, implying that ethylene might play an important role as a signal molecule in tight-regulated shikonin formation. These data contribute to a better understanding of light-involvement in regulating the formation of plant secondary metabolites. (C) 2007 Elsevier GmbH. All rights reserved.
