作者机构：
[Bai Xia; Xiao Hong-bo; Mai Pei; Sun Zhi-liang; Wang Shui-lian; Liu Zhao-ying; Dong Wei; Chen Xiao-jun; Wang Hui; Cai Jie] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Bai Xia; Xiao Hong-bo; Mai Pei; Sun Zhi-liang; Liu Zhao-ying; Dong Wei; Chen Xiao-jun; Wang Hui; Cai Jie] Engn Res Ctr Vet Drugs Hunan Prov, Changsha 410311, Hunan, Peoples R China.
通讯机构：
[Sun Zhi-liang] H;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
关键词：
angiopoietin-like protein 3;clone;pregnancy toxemia;goat
摘要：
Pregnancy toxemia is a metabolic disorder of lipid and glucose. Recent investigations have found that angiopoietin-like protein 3 (ANGPTL3) can contribute to disorder of carbohydrate and lipid metabolism. The present study was conducted to investigate the change of ANGPTL3 expression during pregnancy toxemia. We firstly cloned the full-length cDNA of ANGPTL3 in Liuyang Black goats, revealing that goat ANGPTL3 had the typical structure of the angiopoietin-like family, and its mRNA was exclusively expressed in liver. Pregnancy toxemia of pregnant goat does with twins during late gestation was induced by being fasted for 72 h, and then they were recovered after 5 d of refeeding. Hepatic ANGPTL3 gene expression was significantly down-regulated concomitantly with decreased serum glucose concentration, elevated serum β-hydroxybutyrate and free fatty acid levels in pregnant toxemic goats, and these changes were reversed after refeeding. These results suggest ANGPTL3 may play a certain role in the development of pregnancy toxemia in goats.
摘要：
A Gram-negative, rod-shaped bacterial strain named PB-CS01 which is bioluminescence-positive was isolated from contaminated commercial pork that probably had been exposed to seafood. Optimal growth of strain PB-CS01 requires the presence of 3.0% (w/v) NaCl and a temperature of 20 degrees C. Phylogenetic analysis based on 16S rRNA gene sequences of strain PB-CS01 and other Photobacterium species showed that the novel isolate belongs to the genus Photobacterium. Sequence similarity analysis between PB-CS01 and other species indicates that the closest relatives of strain PB-CS01 are Photobacterium phosphoreum ATCC 11040 (99.9%), Photobacterium kishitanii pjapo.1.1 (99.8%) and Photobacterium iliopiscarium ATCC 51760 (99.5%). The most abundant fatty acids were summed feature 3 (50.77%; C-16:1 omega 7c and/or C-16:1 omega 6c) and C-16:0 (15%). The fatty acid profile is similar to that of the genus Photobacterium but this report is the first to describe C-16:1 omega 6c as one of the compositions of summed feature 3 of the genus Photobacterium. The G+C content of the genomic DNA of strain. PB-CS01 was 44.8 mol%. Overall, strain. PB-CS01 is a novel Photobacterium species.
作者：
Zhang, D. S.;Liu, Z. Y.;Chen, X. J.;Kuang, G. W.;Pan, R. T.;...
期刊：
AFRICAN HEALTH SCIENCES,2012年12(4):522-529 ISSN：1680-6905
通讯作者：
Sun, Z. L.
作者机构：
[Chen, X. J.; Sun, Z. L.; Zhang, D. S.; Pan, R. T.; Liu, Z. Y.] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Kuang, G. W.] Inst Hunan Prov Vet Drugs & Feed Supervis, Changsha 410006, Hunan, Peoples R China.;[Sun, Z. L.] Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Sun, Z. L.] H;Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.
关键词：
Angiopoietin-like protein 3 (ANGPTL3);DNA methylation;Pigs
摘要：
Background: Angiopoietin-like protein 3 (ANGPTL3), a member of angiopoietin-like proteins, has been demonstrated to affect lipid metabolism by inhibiting the activity of lipoprotein lipase (LPL). Objective: To compare the ANGPTL3 mRNA and protein expression, exon mutation and promoter district CpG island methylation state between Ningxiang Pigs and Changbai Pigs. Methods: Pigs were slaughtered and about 100 mg of tissue samples and subcutaneous adipose tissue were collected and stored for analysis. Quantitative Real-Time PCR, Western blotting, exons sequencing, and HRM analysis were carried out. Results: ANGPTL3 was expressed in liver but not in fat and lean meat. Compared with Changbai pigs, ANGPTL3 mRNA level in Ningxiang pigs was lower. However, the protein expression showed no difference between these two groups of pigs. Sequences analysis showed that four variations existed between Changbai Pigs and Ningxiang Pigs, among which three variations caused no change of amino acids, and the other one caused amino acid mutation from Val (Changbai) to Met (Ningxiang). The ANGPTL3 promoter district CpG island bisulfite-PCR and sequencing results showed that the mean methylation rate ranged from 70.952% to 95.238% between Changbai pig and Ningxiang pig (p<0.05). Conclusion: These results support the significant difference (p<0.05) between Changbai pig and Ningxiang pig in high-resolution melting (HRM) analysis. All these results may be helpful for a better understanding of the role of ANGPTL3 in lipid metabolism.
作者机构：
[Xiao, Hong-Bo] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Niu, Xin-Wei] Shunxiang Cow Breeding Co Ltd, Wutaishan 035400, Peoples R China.
通讯机构：
[Xiao, Hong-Bo] H;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
关键词：
Kaempferol;Angiopoietin-like protein 4;Lipoprotein lipase;Steer
摘要：
Previous studies have shown that angiopoietin-like protein 4 (Angptl4) is a protein that stimulates oxidation of fatty acids and inhibits fat accumulation. Kaempferol is a naturally occurring polyphenol agent. The aim of this study was to investigate whether the beneficial effect of kaempferol on carcass characteristics and meat quality in cattle was related to decreased expression of Angptl4. Twenty-seven Holstein steers (Holstein dam x Chinese Yellow sire) (15 months of age) were randomly divided into 3 groups: control group (steers), and two groups of steers treated with kaempferol (0.3 or 0.9%). Ninety days after, kaempferol (0.3 or 0.9%) treatment significantly increased lion eye area, tenderness, backfat thickness, crude fat and expression of lipoprotein lipase (LPL); decreased drip loss, shear forcing, and expression of Angptl4. The present results suggest that kaempferol reduces Angptl4 expression to improve carcass characteristics and meat quality traits in Holstein steers. (c) 2012 Elsevier B.V. All rights reserved.
作者机构：
[Wu, Yong; Sun, Zhi-Liang; Liu, Zhao-Ying] Hunan Agr Univ, Coll Vet Med, Hunan Engn Res Ctr Vet Drug, Changsha 410128, Hunan, Peoples R China.;[Wan, Leren] Shimadzu Int Trading Shanghai Co Ltd, Shanghai 200052, Peoples R China.
通讯机构：
[Liu, Zhao-Ying] H;Hunan Agr Univ, Coll Vet Med, Hunan Engn Res Ctr Vet Drug, Changsha 410128, Hunan, Peoples R China.
关键词：
Diaveridine;Hybrid ion trap/time-of-flight mass spectrometry;Metabolism;Pig liver microsomes;Trimethoprim
摘要：
Trimethoprim (TMP) and diaveridine (DVD) are used in combination with sulfonamides and sulfaquinoxlaine as an effective antibacterial agent and antiprotozoal agent, respectively, in humans and animals. To gain a better understanding of the metabolism of TMP and DVD in the food-producing animals, the metabolites incubated with liver microsomes of pigs were analyzed for the first time with high-performance liquid chromatography combined with hybrid ion trap/time-of-flight mass spectrometry. Seven TMP-related and six DVD-related metabolites were characterized based on the accurate MS2 spectra and known structure of the parent drug, respectively. The metabolites of TMP were identified as two O-demethylation metabolites, a di-O-demethylation metabolite, two N-oxides metabolites, a hydroxylated metabolite on the methylene carbon and a hydroxylated metabolite on the methyl group. DVD was also biotransformed to two O-demethylation metabolites, a di-O-demethylation metabolite, an N-oxide metabolite, a hydroxylation metabolite on the methylene carbon and a hydroxylation metabolite followed by O-demethylation. The results indicate that the two compounds have similar biotransformation pathways in pigs. O-Demethylation was the major metabolic route of TMP and DVD in the pig liver microsomes. The proposed metabolic pathways of TMP and DVD in liver microsomes will provide a basis for further studies of the in vivo metabolism of the two drugs in food-producing animals. Copyright (c) 2011 John Wiley & Sons, Ltd.
