期刊：
International Journal of Mass Spectrometry,2012年309:133-140 ISSN：1387-3806
通讯作者：
Sun, Zhi-Liang
作者机构：
[Sun, Zhi-Liang; Yu, Chun-Hong; Liu, Zhao-Ying] Hunan Agr Univ, Coll Vet Med, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Biol Vet Drugs Branch, Changsha 410128, Hunan, Peoples R China.;[Wan, Leren] Shimadzu Int Trading Shanghai Co Ltd, Shanghai 200052, Peoples R China.
通讯机构：
[Sun, Zhi-Liang] H;Hunan Agr Univ, Coll Vet Med, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Biol Vet Drugs Branch, Changsha 410128, Hunan, Peoples R China.
关键词：
Trichothecenes;Ion trap/time-of-flight mass spectrometry;Accurate mass;Fragmentation
摘要：
Five trichothecenes, T-2 toxin, HT-2 toxin, deoxynivalenol (DON), 3-acetyl-DON (3-AcDON) and nivalenol (NIV), major class of mycotoxins produced by Fusarium, were investigated by electrospray hybrid ion trap/time-of-flight mass spectrometry (IT–TOF-MS). Fragmentation mechanisms are proposed based on the MS2 and MS3 experiments and accurate mass measurements. The sodium adduct ions [M+Na]+ of type-A trichothecenes, such as T-2 toxin and HT-2 toxin, were observed in the MS spectra. However, the [M−H]− ions of type-B trichothecenes, such as NIV, and 3-AcDON, were observed in the MS spectra. Eliminations of isovaleryloxy group and acetic acid were the common fragmentation pathways of T-2 toxin and HT-2 toxin. However, the cleavage of epoxy was the common fragmentation pathways of DON, 3-AcDON and NIV. The other fragmentation pathways were found to be dependent on the substitutent group. The data reported here may help to identify analogues of the trichothecenes that are commonly co-produced by Fusarium in cultures or naturally contaminated samples.
摘要：
In this study, the stress degradation of olaquindox under conditions of hydrolysis (neutral, acidic and basic), oxidation and photolytic stress was investigated. In order to characterize each degradation product, we developed a rapid, sensitive and reliable high-performance liquid chromatography combined with hybrid ion trap/time-of-flight mass spectrometry (LC/MS-IT-TOF) method. The degradation products formed under different forced conditions were separated using an ODS-C18 column with gradient elution. Multiple scans of degradation products in MS and MS/MS modes and accurate mass measurements were performed through data-dependent acquisition. The structural elucidations of degradation products were performed by comparing the changes in the accurate molecular masses and fragment ions generated from precursor ions with those of parent drug. The present results showed that maximum degradation was observed in hydrolysis, especially in the acidic condition. The drug was also degraded significantly under photolytic conditions. A total of 12 degradation products of olaquindox were detected and characterized using the developed method. The main degradation product was formed by the complete cleavage of side chain to form 3-methyl-2-hydroxylquinoxaline-4-oxide. A degradation pathway of olaquindox was also tentatively proposed for the first time based on these characterized structures. (C) 2011 Elsevier B.V. All rights reserved.
期刊：
Toxicology and Applied Pharmacology,2011年257(3):405-411 ISSN：0041-008X
通讯作者：
Xiao, Hong-Bo
作者机构：
[Xiao, Hong-Bo] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Zhang, Heng-Bo] Furong Dist Red Cross Hosp, Changsha 410126, Peoples R China.
通讯机构：
[Xiao, Hong-Bo] H;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
关键词：
Apolipoprotein e-deficient mouse;Cluster of differentiation 44;Inflammation;Kaempferol;Osteopontin
摘要：
Recent studies show that osteopontin (OPN) and its receptor cluster of differentiation 44 (CD44) are two pro-inflammatory cytokines contributing to the development of atherosclerosis. The objective of this study was to explore the inhibitory effect of kaempferol, a naturally occurring flavonoid compound, on atherogenesis and the mechanisms involved. The experiments were performed in aorta and plasma from C57BL/6J control and apolipoprotein E-deficient (ApoE(-/-)) mice treated or not with kaempferol (50 or 100 mg/kg, intragastrically) for 4 weeks. Kaempferol treatment decreased atherosclerotic lesion area, improved endothelium-dependent vasorelaxation, and increased the maximal relaxation value concomitantly with decrease in the half-maximum effective concentration, plasma OPN level, aortic OPN expression, and aortic CD44 expression in ApoE(-/-) mice. In addition, treatment with kaempferol also significantly decreased reactive oxygen species production in mice aorta. The present results suggest that kaempferol regulates OPN-CD44 pathway to inhibit the atherogenesis of ApoE(-/-) mice. (C) 2011 Elsevier Inc. All rights reserved.
摘要：
Conjugates of the Escherichia coli serum antibody (Ab) with the gentamicin sulfate (GM sulfate) have been evaluated for the assessment of their ability to eradicate E. coli in vitro. The combination of every component in the targeted drug is the amino of serum antibody and gentamicin sulfate combined with the hydroxyl of PEG6000 by hydrogen bond. which forms stable complexes. The half-life of this complex is 4.83 h, and it is non-toxic side effects and low immunogenic. After the combination of antibody and gentamicin sulfate, the targeting of antibody is quite good. In vitro anti-bacterial activity of gentamicin sulfate increased 1000 times, and the clinical curative effect enhanced 100 times, this means higher efficacy and safety. (C) 2011 Elsevier Ltd. All rights reserved.
