Prevalence and antimicrobial susceptibility of Clostridium perfringens in chickens and pigs from Beijing and Shanxi, China
作者:
Li, Jiyun;Zhou, Yuqing;Yang, Dawei;Zhang, Shan;Sun, Zhiliang;...
期刊:
Veterinary Microbiology ,2021年252:108932 ISSN:0378-1135
通讯作者:
Wang, Shaolin;Wu, Congming
作者机构:
[Wang, Yang; Wu, Congming; Wang, Shaolin; Wu, CM; Zhou, Yuqing; Li, Jiyun; Zhang, Shan] China Agr Univ, Coll Vet Med, Beijing Key Lab Detect Technol Anim Derived Food, Beijing, Peoples R China.;[Sun, Zhiliang; Li, Jiyun] Hunan Agr Univ, Coll Vet Med, Changsha, Hunan, Peoples R China.;[Zhou, Yuqing] Cardiff Univ, Dept Med Microbiol & Infect Dis, Div Infect & Immun, Cardiff, Wales.;[Wang, Yang; Yang, Dawei] China Agr Univ, Coll Vet Med, Beijing Lab Food Qual & Safety, Beijing, Peoples R China.
通讯机构:
[Wang, SL; Wu, CM] C;China Agr Univ, Coll Vet Med, Beijing Key Lab Detect Technol Anim Derived Food, Beijing, Peoples R China.
关键词:
Clostridium perfringens;Prevalence;Antimicrobial susceptibility;Chickens;Pigs;Whole genome sequencing
摘要:
The prevalence and antimicrobial susceptibility of Clostridium perfringens (C. perfringens) in chickens and pigs were investigated in Beijing and Shanxi, China. In total, 322 C. perfringens (chicken n = 60 and pig n = 262) were obtained from 620 feces of chickens (n = 256) and pigs (n = 364). Multiplex PCR for toxin typing of C. perfringens revealed that all the isolates belong to type A, with 45.7 % (147/322) isolates carrying beta-2 toxin-encoding gene cpb2. Minimum inhibitory concentrations of 27 antimicrobial agents showed that 91.0 % of the tested C. perfringens isolates were resistant to gentamicin and sulfonamides (sulfisoxazole and trimethoprimsulfamethoxazole), and little resistance was showed to amoxicillin-clavulanate, ceftiofur, doxycycline, vancomycin and linezolid. Additionally, nosiheptide, avilamycin, virginiamycin and bacitracin exhibited good activity against the tested C. perfringens with low MIC50 (0.06 to <= 4 mu g/mL) and MIC90 values (0.25-8 mu g/mL). Whole genome sequencing (WGS) of 48 representative isolates from each farm indicated that the C. perfringens contained diverse antimicrobial resistance genes [tetA(P), ant(6)-Ib, erm(Q), etc.] and toxin genes (cpb2, colA, cloSI, pfoA, etc.). By comparative analysis, four C. perfringens isolates from three different pig farms harboured cpb2-carrying plasmid pl with 100 % nucleotide sequence identity, suggesting horizontal gene transfer among these microorganisms. The further phylogenomic reconstruction, based on the core-genome single nucleotide polymorphisms (SNPs) of the representatives, demonstrating that C. perfringens from the same farms and regions were closely related. These findings expanded our knowledge of C. perfringens isolated from animals in China, which provided scientific basis for efficient intervention or prevention measures of antimicrobial resistance in animal husbandry in China.
语种:
英文
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关于建立健全动物疫病防控体系的对策研究
作者:
杨清;孙志良
期刊:
兽医导刊 ,2020年(12):47-47 ISSN:1673-8586
作者机构:
湖南农业大学 动物医学院,湖南长沙 410128;[杨清; 孙志良] 湖南农业大学
关键词:
动物防疫体系;意义;对策研究
摘要:
在当前严峻的动物疫病防控形势下,建立健全动物疫病防控体系对促进养殖业转型升级,保障养殖业健康发展;促进畜禽产品稳产保供,保障老百姓的"菜篮子";促进农村经济发展,保障农民增产增收,促进人兽共患病防控,保障公共卫生安全等方面有着重要意义.建立健全动物疫病防控体系应从以下3方面着手:⑴加大兽医社会化服务模式的扶持力度;⑵大力发展兽医协会,加强与高校合作;⑶创新激励机制,落实企业责任制.
语种:
中文
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猪流行性腹泻及新型疫苗研究进展
作者:
郭思杰;刘俊琦;李玉娟;孙志良
期刊:
中国畜牧兽医 ,2020年47(9):2935-2944 ISSN:1671-7236
作者机构:
湖南农业大学动物医学院;[郭思杰; 刘俊琦; 孙志良; 李玉娟] 湖南省兽药工程技术研究中心
关键词:
猪流行性腹泻(PED);发病机制;新型疫苗
摘要:
猪流行性腹泻(porcine epidemic diarrhea,PED)是危害养猪业健康发展的重要疾病之一,具有急性、高度传染性的特征,给养猪业造成了严重的经济损失。猪流行性腹泻病毒(porcine epidemic diarrhea virus,PEDV)感染会破坏动物机体的消化系统,造成患病猪食欲下降、呕吐以及严重腹泻等,且药物治愈后的仔猪也会因为生长发育不良等因素严重影响生产,给养殖户带来巨大的困扰。2010年PEDV高致病变异毒株在中国暴发流行,导致PED的发病率和死亡率大幅升高,严重影响中国养猪生产。此次PED的暴发流行引起了养猪行业的密切关注,相关研究领域的学者对PEDV致病机制和PED新型疫苗进行了更加深入的研究,亚单位疫苗、病毒活载体疫苗、细菌活载体疫苗、转基因植物疫苗和核酸疫苗5种PED新型疫苗相继被开发,并取得全新的突破和进展。与传统的PED灭活苗和PED弱毒疫苗相比,PED新型基因工程疫苗具有安全性好、制备简单、免疫效果好等优点。文章着重对PEDV发病机理和5种PED新型疫苗的研究进展展开综述,从而加深对PEDV和PED新型疫苗的了解,以期为PEDV感染的预防和控制措施提供参考。
语种:
中文
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湖南尖吻蝮蛇蛇毒蛋白组分分析及毒性探索性研究
作者:
肖纲;刘俊琦;张志阳;夏雨;孙志良
作者机构:
湖南省兽药工程技术研究中心湖南农业大学动物医学院
会议名称:
中国畜牧兽医学会兽医药理毒理学分会第十五次学术讨论会
会议时间:
2019-10-13
会议地点:
中国甘肃兰州
关键词:
尖吻蝮蛇蛇毒;蛇毒金属蛋白
摘要:
[目的]尖吻蝮蛇蛇毒具有极强的毒性,其含有多种不同生物学活性的蛋白质、多肽、酶等。本研究旨在通过对湖南省永州市尖吻蝮蛇蛇毒的蛋白成分分析和毒性进行初步探索研究,为湖南尖吻蝮蛇蛇毒的毒性及医药应用提供重要的参考依据。[方法]本研究采用Nano-LC-ESI-MS/MS技术对湖南尖吻蝮蛇蛇毒蛋白组分进行定性分析,采用常规体外试管法对尖吻蝮蛇蛇毒体外溶血值进行检测,采用改良寇式法对KM小鼠单剂量皮下注射尖吻蝮蛇蛇毒的LD50进行测定。[结果]结果显示尖吻蝮蛇蛇毒含有50种蛋白,蛋白相对分子量主要在10-50KD,其中高丰度蛋白质为蛇毒金属蛋白、类凝血酶和抗凝血酶;尖吻蝮蛇蛇毒引起体外红细胞溶血的最低浓度为0.06mg/mL,对KM小鼠的LD50为7.18mg/kg,攻毒小鼠出现呼吸急促、躁动不安,注射部位出现奇特瘙痒,反复抓挠,至注射部位出现大面积溃烂,直至死亡。[结论]根据本研究从尖吻蝮蛇蛇毒中鉴定出的50种蛋白,体外溶血值和LD50的准确测定,可对尖吻蝮蛇蛇毒中发挥重要作用的蛋白有初步的判断,可为进一步研究尖吻蝮蛇蛇毒的毒理机制提供宝贵的基础数据。本研究为湖南尖吻蝮蛇蛇毒毒性评价及药用开发与利用奠定基础。
语种:
中文
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钩吻素甲在猪肝S9中的代谢产物鉴定
作者:
俞辉;肖洒;黄亚军;孙志良;白霞;...
期刊:
中兽医医药杂志 ,2019年38(2):5-8 ISSN:1000-6354
作者机构:
湖南省兽药工程技术研究中心湖南农业大学动物医学院,湖南长沙,410128;[白霞; 俞辉; 刘兆颖; 黄亚军; 肖洒; 孙志良] 湖南农业大学
关键词:
钩吻素甲;代谢;钩吻;猪;肝
摘要:
研究钩吻素甲在猪体外肝S9的代谢.采用高效液相色谱串联四极杆飞行时间质谱(HPLC/QqTOFMS)对孵育后的代谢物进行结构鉴定.结果 表明,钩吻素甲在猪肝S9中仅检出2种代谢产物,根据原形及其代谢物的精确分子质量差和产物离子,鉴定了这2种代谢物结构,进而表明钩吻素甲的主要代谢途径是氧化和去甲基.
语种:
中文
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黄连素的生物学功能及其在动物生产中的应用
作者:
郭思杰;文伟;杨昆;孙志良
期刊:
动物营养学报 ,2019年31(10):4464-4472 ISSN:1006-267X
作者机构:
湖南农业大学动物医学院, 长沙, 410128;湖南省兽药工程技术中心, 湖南省兽药工程技术中心, 长沙, 410128;湖南农业大学动物科学技术学院, 长沙, 410128;[郭思杰; 杨昆; 孙志良] 湖南省兽药工程技术研究中心;[文伟] 湖南农业大学
关键词:
黄连素;抗氧化;抗菌;抗炎;饲料添加剂
摘要:
黄连素是从草本植物中提取的主要有效化学成分,具有抗菌、抗炎、抗氧化以及调节脂质代谢等生物学功能,其作为天然饲料添加剂的一种,在提高动物生产性能、调节肠道菌群平衡以及改善产品肉品质等方面效果显著,因而在畜禽生产中拥有广阔的应用前景。本文就黄连素的理化性质、提取工艺、生物学功能及其在畜禽生产中的应用展开综述,以期为黄连素在动物生产应用中提供参考依据。
语种:
中文
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饲料添加钩吻干粉对仔猪生长性能的影响及机制
作者:
王颉;孙志良
期刊:
中兽医学杂志 ,2019年(2):3-4 ISSN:1003-8655
作者机构:
湖南农业大学动物医学院,湖南长沙,410128;[王颉; 孙志良] 湖南农业大学
关键词:
钩吻;仔猪;生长性能;血液生理生化指标;基因表达
摘要:
目的 钩吻对畜禽具有良好的促生长作用,但其具体机制尚不清楚.本文研究钩吻对断奶仔猪生长性能、血液生理生化指标及肝脏相关基因表达的影响.方法 30头56日龄的仔猪按体重平均分为两组,对照组(CON)饲喂基础日粮,钩吻组饲喂补充50mg/kg剂量的钩吻醇提物日粮.试验期为49天,每两周记录一次体重和采食量;试验结束后,采血液和肝脏组织进行相关基因检测.结果 试验结果显示,日粮补充钩吻干粉显著提高了仔猪的平均日增重(11%),并降低料重比(12%)(P<0.05).钩吻显著提升了仔猪血液红细胞数量(7.6%)、血红蛋白(8.4%)、血清总蛋白(5.9%)、免疫球蛋白A(36%)、胰岛素(11%)和尿素氮(17%)等指标(P<0.05),同时降低血清中谷丙转氨酶(15%)和谷草转氨酶(23%)活性以及谷丙转氨酶与谷草转氨酶比值(P<0.05).同时,钩吻显著增加肝脏PPARD、ANGPT4和HMGCS2等基因的mRNA丰度(P<0.05),降低PDK4基因的表达量(P<0.05).结论 钩吻作为饲料添加剂能够显著提高仔猪生长性能,其促生长作用可能与其参与血液生化参数以及糖脂代谢相关基因的表达有关.
语种:
中文
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Gene expression profile analysis of ileum transcriptomes in pigs fed Gelsemium elegans plants.
作者:
Huang, Chong-Yin;Yang, Kun;Cao, Jun-Jie;Li, Yu-Juan;Wang, Zi-Yuan;...
期刊:
Scientific Reports ,2019年9(1):15756 ISSN:2045-2322
通讯作者:
Liu, Zhao-Ying
作者机构:
[Yu, Hui; Wang, Zi-Yuan; Huang, Chong-Yin; Li, Yu-Juan; Sun, Zhi-Liang; Zheng, Xiaofeng; Yang, Kun; Liu, Zhao-Ying; Cao, Jun-Jie] Hunan Agr Univ, Hunan Engn Technol Res Ctr Vet Drugs, Changsha 410128, Hunan, Peoples R China.;[Yu, Hui; Wang, Zi-Yuan; Huang, Chong-Yin; Li, Yu-Juan; Sun, Zhi-Liang; Zheng, Xiaofeng; Yang, Kun; Liu, Zhao-Ying; Cao, Jun-Jie] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
通讯机构:
[Liu, Zhao-Ying] H;Hunan Agr Univ, Hunan Engn Technol Res Ctr Vet Drugs, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
摘要:
Gelsemium elegans is a flowering plant in the Loganiaceae. Because it can promote the growth of pigs and sheep, it is widely used, including in veterinary clinics, but little information is available about its biological effects. Here, we used high-throughput sequencing to characterize the differentially expressed genes (DEGs) in the ileums of pigs between a control group and a group fed Gelsemium elegans for 45 days. We found that Gelsemium elegans affected many inflammatory and immune pathways, including biological processes such as defense responses, inflammation and immune responses. Moreover, this study identified several important genes related to the anti-inflammatory activity of Gelsemium elegans (e.g., CXCL-8, IL1A, and CSF2), which will be beneficial for further study of the pharmacological mechanisms and clinical applications of Gelsemium elegans.
语种:
英文
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Protective Effect of Koumine, an Alkaloid from Gelsemium Sempervirens, on Injury Induced by H2O2 in IPEC-J2 Cells
作者:
Yuan, Zhihang;Liang, Zengenni;Yi, Jine;Chen, Xiaojun;Li, Rongfang;...
期刊:
International Journal of Molecular Sciences ,2019年20(3) ISSN:1422-0067
通讯作者:
Wu, Jing;Sun, Zhiliang
作者机构:
[Wu, Yong; Wu, Jing; Chen, Xiaojun; Li, Rongfang; Yi, Jine; Sun, Zhiliang; Yuan, Zhihang; Wu, J] Hunan Agr Univ, Coll Vet Med, Dept Clin Vet Med, Changsha 410128, Hunan, Peoples R China.;[Wu, Yong; Wu, Jing; Chen, Xiaojun; Li, Rongfang; Yi, Jine; Sun, Zhiliang; Yuan, Zhihang; Wu, J] Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha 410128, Hunan, Peoples R China.;[Liang, Zengenni] Dept Hunan Agr Prod Proc Inst, Changsha 410128, Hunan, Peoples R China.
通讯机构:
[Wu, J; Sun, ZL] H;Hunan Agr Univ, Coll Vet Med, Dept Clin Vet Med, Changsha 410128, Hunan, Peoples R China.;Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha 410128, Hunan, Peoples R China.
关键词:
H2O2;IPEC-J2 cells;apoptosis;koumine
摘要:
Medicinal herbal plants have been commonly used for intervention in different diseases and improvement of health worldwide. Koumine, an alkaloid monomer found abundantly in Gelsemium plants, can be effectively used as an antioxidant. The purpose of this study was to evaluate the potential protective effect of koumine against hydrogen peroxide (H(2)O(2))-induced oxidative stress and apoptosis in porcine intestinal epithelial cell line (IPEC-J2 cells). MTT assays showed that koumine significantly increased cell viability in H(2)O(2)-mediated IPEC-J2 cells. Preincubation with koumine ameliorated H(2)O(2)-medicated apoptosis by decreasing reactive oxygen species (ROS) production, and efficiently suppressed the lactate dehydrogenase (LDH) release and malondialdehyde (MDA) production. Moreover, a loss of superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) activities was restored to normal level in H(2)O(2)-induced IPEC-J2 cells upon koumine exposure. Furthermore, pretreatment with koumine suppressed H(2)O(2)-mediated loss of mitochondrial membrane potential, caspase-9 and caspase-3 activation, decrease of Bcl-2 expression and elevation of Bax expressions. Collectively, the results of this study indicated that koumine possesses the cytoprotective effects in IPEC-J2 cells during exposure to H(2)O(2) by suppressing production of ROS, inhibiting the caspase-3 activity and influencing the expression of Bax and Bcl-2. Koumine could potentially serve as a protective effect against H(2)O(2)-induced apoptosis.
语种:
英文
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A novel two-dimensional liquid chromatography system for the simultaneous determination of three monoterpene indole alkaloids in biological matrices
作者:
Liu, Sha-Sha;Yang, Kun;Sun, Zhi-Liang;Zheng, Xiaofeng;Bai, Xia;...
期刊:
Analytical and Bioanalytical Chemistry ,2019年411(17):3857-3870 ISSN:1618-2642
通讯作者:
Liu, Zhao-Ying
作者机构:
[Bai, Xia; Liu, Sha-Sha; Sun, Zhi-Liang; Zheng, Xiaofeng; Yang, Kun; Liu, Zhao-Ying] Hunan Agr Univ, Hunan Engn Technol Res Ctr Vet Drugs, Changsha 410128, Hunan, Peoples R China.;[Bai, Xia; Liu, Sha-Sha; Sun, Zhi-Liang; Zheng, Xiaofeng; Yang, Kun; Liu, Zhao-Ying] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
通讯机构:
[Liu, Zhao-Ying] H;Hunan Agr Univ, Hunan Engn Technol Res Ctr Vet Drugs, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
关键词:
Two-dimensional liquid chromatography;Gelsemium;Indole alkaloid;Gelsemine;Koumine;Humantenmine
摘要:
The present paper describes a novel two-dimensional liquid chromatography (2D-LC) system, which is comprised of a first-dimensional ion exchange chromatography (IEX1) column, trap column, and second-dimensional reversed-phase chromatography (RP2) column system. The biological sample is separated by the first-dimensional LC using an IEX column to remove interferences. The analytes are transferred to the trap column after heart-cutting. Then, the analytes are transferred to the second-dimensional LC using an RP2 column for further separation and ultraviolet detection. This 2D-LC system can offer a large injection volume to provide sufficient sensitivity and exhibits a strong capacity for removing interferences. Here, the determination of three monoterpene indole alkaloids (MIAs; gelsemine, koumine, and humantenmine) from Gelsemium in biological matrices (plasma, tissue, and urine) was used this 2D-LC system. After a rapid and easy sample preparation method based on protein precipitation, the sample was injected into the 2D-LC. The method was developed and validated in terms of the selectivity, LOD, LOQ, linearity, precision, accuracy, and stability. The sample preparation time for the three MIAs was 15min. The LOD for these compounds was 10ng/mL, which was lower than the developed HPLC methods. The results showed that this method had good quantitation performance and allowed the determination of gelsemine, koumine, and humantenmine in biological matrices. The method is rapid, exhibits high selectivity, has good sensitivity, and is low-cost, thus making it well-suited for application in the pharmaceutical and toxicological analysis of Gelsemium. [Figure not available: see fulltext.]. © 2019, Springer-Verlag GmbH Germany, part of Springer Nature.
语种:
英文
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湖南永州尖吻蝮蛇蛇毒的蛋白组分及毒性分析
作者:
肖纲;刘俊琦;夏雨;张志阳;孙志良
期刊:
湖南农业大学学报(自然科学版) ,2019年45(5):541-547 ISSN:1007-1032
作者机构:
湖南农业大学动物医学院,湖南 长沙,410128;湖南农业大学动物医学院,湖南 长沙 410128;湖南省兽药工程技术研究中心,湖南 长沙 410128;[肖纲; 张志阳; 刘俊琦; 夏雨; 孙志良] 湖南农业大学
关键词:
尖吻蝮蛇蛇毒;蛇毒金属蛋白;半数致死量;湖南永州
摘要:
采用Nano–LC–ESI–MS/MS蛋白鉴定技术对湖南永州尖吻蝮蛇蛇毒蛋白组分进行质谱分析,应用常规体外试管法对尖吻蝮蛇蛇毒体外溶血值进行检测,采用改良寇式法对尖吻蝮蛇蛇毒LD_(50)进行测定。结果显示:尖吻蝮蛇蛇毒含有50种蛋白,蛋白相对分子质量集中在1.0×10~4~2.0×10~4(46.9%)、4.0×10~4~5.0×10~4(22.4%)、 2.0×10~4~3.0×10~4(10.6%)和7.0×10~4~8.0×10~4(7.6%),其中代表性的高丰度蛋白有蛇毒金属蛋白酶A(11.7%)、蛇毒金属蛋白酶H1(9.8%)、蕲蛇类凝血酶-2(7.3%)、抗凝血酶A-A亚基(6.8%),低丰度蛋白(<0.1%)有Ecto-5'-核苷酸酶、碱性磷脂酶A2 DAV-N6、生长分化因子11;蛇毒引起红细胞溶血的最低质量浓度为60.00 μg/mL,尖吻蝮蛇蛇毒对KM小鼠的LD_(50)为7.179 6 mg/kg,攻毒小鼠出现呼吸急促、躁动不安、注射部位出现奇特瘙痒和大面积溃烂,至死亡。
语种:
中文
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An analytical strategy to explore the multicomponent pharmacokinetics of herbal medicine independently of standards: Application in Gelsemium elegans extracts
作者:
Yang, Kun;Long, Xue-Ming;Cao, Jun-Jie;Li, Yu-Juan;Wu, Yong;...
期刊:
Journal of Pharmaceutical and Biomedical Analysis ,2019年176:112833 ISSN:0731-7085
通讯作者:
Sun, Zhi-Liang;Liu, Zhao-Ying
作者机构:
[Wu, Yong; Bai, Xia; Li, Yu-Juan; Sun, Zhi-Liang; Yang, Kun; Liu, Zhao-Ying; Cao, Jun-Jie] Hunan Agr Univ, Hunan Engn Technol Res Ctr Vet Drugs, Changsha 410128, Hunan, Peoples R China.;[Wu, Yong; Bai, Xia; Li, Yu-Juan; Sun, Zhi-Liang; Yang, Kun; Liu, Zhao-Ying; Cao, Jun-Jie] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Long, Xue-Ming] Hunan Prov Inst Vet Drugs & Feed Control, Changsha 410006, Hunan, Peoples R China.;[Sun, ZL; Liu, Zhao-Ying] Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.
通讯机构:
[Sun, ZL; Liu, ZY] H;Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.
关键词:
Alkaloids;Gelsemium elegans;Herbal medicine;LC-MS/MS;Pharmacokinetics
摘要:
The multicomponent pharmacokinetic study of herbal medicine is a great challenge due to the low plasma concentrations, large range of concentration scales, lack of authentic standards and uncertain interactions of the components. The aim of this work was to explore the in vivo pharmacokinetics of herbal medicine independently of authentic standards using an integrated analytical strategy. First, ion pairs of multiple components were tuned and selected, and then major parameters were optimized for derivative multiple reaction monitoring (DeMRM) by LC-MS/MS, which was combined with characterization of the chemical profiles of the herbal medicine by LC-QqTOF/MS. Second, different concentrations of herbal extracts were employed instead of authentic standards to construct calibration curves for the semiquantitative determination of multiple components in plasma. Taking Gelsemium elegans as an example, in addition to the fully validated and sufficient methodological results, a total of 27 alkaloid components, major bioactive constituents of Gelsemium elegans, were simultaneously monitored in pig plasma. The concentration-time profiles and pharmacokinetic properties of these 27 components were characterized. The absolute quantification of three components was compared with the results obtained using authentic standards, and the method showed very similar analytical characteristics, such as linearity, precision, accuracy, and the values of the pharmacokinetic parameters Tmax, Vd, Cl and MRT. This analytical strategy was found to be capable of assessing herbal pharmacokinetics independently of specific authentic compounds for each component. This study was the first attempt to systematically reveal the in vivo pharmacokinetics of Gelsemium elegans. This strategy and methodology will find widespread use in the quantitative pharmacokinetic analysis of multiple components independently of standards for herbal medicine, among other applications.
语种:
英文
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Koumine promotes ROS production to suppress hepatocellular carcinoma cell proliferation via NF-κB and ERK/p38 MAPK signaling
作者:
Yuan, Zhihang;Liang, Zengenni;Yi, Jine;Chen, Xiaojun;Li, Rongfang;...
期刊:
Biomolecules ,2019年9(10) ISSN:2218-273X
通讯作者:
Wu, Jing;Sun, Zhiliang
作者机构:
[Wu, Jing; Chen, Xiaojun; Li, Rongfang; Yi, Jine; Sun, Zhiliang; Yuan, Zhihang] Hunan Agr Univ, Coll Vet Med, Dept Clin Vet Med, Changsha 410128, Hunan, Peoples R China.;[Wu, Jing; Chen, Xiaojun; Li, Rongfang; Yi, Jine; Sun, Zhiliang; Yuan, Zhihang; Wu, J] Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha 410128, Hunan, Peoples R China.;[Liang, Zengenni] Dept Hunan Agr Prod Proc Inst, Changsha 410128, Hunan, Peoples R China.
通讯机构:
[Wu, J; Sun, ZL] H;Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha 410128, Hunan, Peoples R China.
关键词:
NF-kappaB and ERK/p38 MAPK signaling;ROS;hepatocellular carcinoma (HCC);koumine;proliferation
摘要:
In the past decades, hepatocellular carcinoma (HCC) has been receiving increased attention due to rising morbidity and mortality in both developing and developed countries. Koumine, one of the significant alkaloidal constituents of Gelsemium elegans Benth., has been regarded as a promising anti-inflammation, anxiolytic, and analgesic agent, as well as an anti-tumor agent. In the present study, we attempted to provide a novel mechanism by which koumine suppresses HCC cell proliferation. We demonstrated that koumine might suppress the proliferation of HCC cells and promote apoptosis in HCC cells dose-dependently. Under koumine treatment, the mitochondria membrane potential was significantly decreased while reactive oxygen species (ROS) production was increased in HCC cells; in the meantime, the phosphorylation of ERK, p38, p65, and IκBα could all be inhibited by koumine treatment dose-dependently. More importantly, the effects of koumine upon mitochondria membrane potential, ROS production, and the phosphorylation of ERK, p38, p65, and IκBα could be significantly reversed by ROS inhibitor, indicating that koumine affects HCC cell fate and ERK/p38 MAPK and NF-κB signaling activity through producing excess ROS. In conclusion, koumine could inhibit the proliferation of HCC cells and promote apoptosis in HCC cells; NF-κB and ERK/p38 MAPK pathways could contribute to koumine functions in a ROS-dependent manner. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
语种:
英文
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Procyanidin B2 from lotus seedpod regulate NO/ADMA/DDAH pathway to treat insomnia in rats
作者:
Xiao, Hong-Bo* ;Wang, Yi-Shan;Liang, Lin;Lu, XiangYang;Sun, Zhi-Liang
期刊:
Fundamental & Clinical Pharmacology ,2019年33(5):549-557 ISSN:0767-3981
通讯作者:
Xiao, Hong-Bo
作者机构:
[Xiao, Hong-Bo; Wang, Yi-Shan; Liang, Lin] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Lu, XiangYang] Hunan Agr Univ, Hunan Prov Univ Key Lab Agr Biochem & Biotransfor, Changsha 410128, Hunan, Peoples R China.;[Lu, XiangYang] Hunan Coinnovat Ctr Ultilizat Bot Funct Ingredien, Changsha 410128, Hunan, Peoples R China.;[Sun, Zhi-Liang] Hunan Agr Univ, Hunan Engn Res Ctr Vet Drug, Changsha 410128, Hunan, Peoples R China.
通讯机构:
[Xiao, Hong-Bo] H;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
关键词:
Lotus seedpod procyanidins;asymmetric dimethylarginine;dimethylarginine dimethylaminohydrolase;insomnia;oxidative stress
摘要:
Recent studies show that nitric oxide/asymmetric dimethylarginine/dimethylarginine dimethylaminohydrolase (NO/ADMA/DDAH) pathway may contribute to the development of sleep disorder. The objective of this study was to explore the inhibitory effect of procyanidin B2 from lotus seedpod (LSPC), a naturally occurring catechin compound, on insomnia and the mechanisms involved. The experiments were performed in brain from Sprague-Dawley rat control and insomniac rats treated or not with LSPC (15, 30, and 45 mg/kg, intragastrically) for 7 days. LSPC treatment reduced walking time and forelimb lifting-up frequency, cerebral levels of noradrenaline, glutamic acid, ADMA, sleep latency, and 8-isoprostane; increased sleep duration, cerebral concentrations of 5-hydroxytryptamine, γ-aminobutyric acid, and NO concomitantly with upregulated cerebral expression of DDAH 1, DDAH2, and neuronal NO synthases in insomniac rats. The present results suggest that LSPC may regulate NO/ADMA/DDAH pathway by inhibiting oxidative stress to treat insomnia in rats when sleep evaluation was achieved on the basis of behavioral criteria. © 2019 Société Française de Pharmacologie et de Thérapeutique
语种:
英文
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钩吻素子在猪的体外代谢
作者:
肖洒;黄亚军;刘艳纯;孙志良;刘兆颖
期刊:
中国兽医学报 ,2018年38(8):1568-1572 ISSN:1005-4545
作者机构:
[肖洒; 黄亚军; 刘艳纯; 孙志良; 刘兆颖] 湖南农业大学动物医学院, 湖南省兽药工程技术研究中心, 湖南, 长沙, 410128
关键词:
钩吻素子;猪;体外代谢
摘要:
研究钩吻素子在猪体外肝S9的代谢,采用高效液相色谱串联四极杆飞行时间质谱(HPLC/QqTOF MS)对孵育后的代谢物进行结构鉴定。结果表明,钩吻素子在猪肝S9中可生成7种代谢产物,根据原形及其代谢物的精确分子质量差、产物离子,鉴定了代谢物结构,包括N-脱甲基化代谢物(M1)、C18和C19的加氢代谢物(M3)、M3的去甲基代谢物(M4)、氧化代谢物(M6、M8和M12)和脱氢代谢物(M13)。钩吻素子在猪的代谢途径主要是氧化、还原、 N-脱甲基化。其研究结果为钩吻素子在动物及人体内代谢研究奠定基础。
语种:
中文
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Naringin Activates AMPK Resulting in Altered Expression of SREBPs, PCSK9, and LDLR To Reduce Body Weight in Obese C57BL/6J Mice
作者:
Sui, Guo-Guang;Xiao, Hong-Bo* ;Lu, Xiang-Yang;Sun, Zhi-Liang
期刊:
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ,2018年66(34):8983-8990 ISSN:0021-8561
通讯作者:
Xiao, Hong-Bo
作者机构:
[Sui, Guo-Guang; Xiao, Hong-Bo] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Lu, Xiang-Yang] Hunan Agr Univ, Hunan Prov Univ Key Lab Agr Biochem & Biotransfor, Changsha 410128, Hunan, Peoples R China.;[Lu, Xiang-Yang] Hunan Coinnovat Ctr Ultilizat Bot Funct Ingredien, Changsha 410128, Hunan, Peoples R China.;[Sun, Zhi-Liang] Hunan Engn Res Ctr Vet Drug, Changsha 410128, Hunan, Peoples R China.
通讯机构:
[Xiao, Hong-Bo] H;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
关键词:
naringin;AMP-activated protein kinase;obesity;C57BL/6J
摘要:
Previous investigations have shown molecular cross-talk among activated adenosine monophosphate-activated protein kinase (AMPK), proprotein convertase subtilisin/kexin type 9 (PCSK9), sterol regulatory element-binding proteins (SREBPs), and low-density lipoprotein receptor (LDLR) and that it may be an innovative pharmacologic objective for treating obesity. We scrutinized the beneficial effect of naringin, a flavanone-7-O-glycoside, on obesity and the mechanisms in the present study. We arbitrarily divided 50 mice into five groups (n = 10): 25 or 50 or 100 mg/kg/day naringin-treated obese mice (gavage for 8 weeks), untreated obese mice, and C57BL/6J control. After 8 weeks, body weight was 51.8 ± 4.4 in the untreated obese mice group, while the weights were 41.4 ± 4.1, 34.6 ± 2.2, and 28.0 ± 2.3 in 25, 50,100 mg/kg naringin groups, respectively. Moreover, naringin treatment significantly decreased plasma 8-isoprostane (an indicator of the oxidative stress) level, fat weight, liver weight, hepatic total cholesterol concentration, hepatic triglyceride concentration, plasma leptin level, plasma insulin content, plasma low-density lipoprotein cholesterol level, and plasma PCSK9 production concomitantly with down-regulated expression of SREBP-2, PCSK9, and SREBP-1, and up-regulated expression of p-AMPKα and LDLR. The present results suggest that naringin activates AMPK resulting in altered expression of SREBPs, PCSK9, and LDLR to reduce the body weight of obese C57BL/6J mice. © 2018 American Chemical Society.
语种:
英文
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高效液相法检测鸡盲肠中的沙咪珠利
作者:
易远胜;郭春娜;王霄旸;张可煜;杨帆帆;...
期刊:
中国动物传染病学报 ,2018年26(2):80-84 ISSN:1674-6422
作者机构:
湖南农业大学动物医学院,长沙 410000;湖南农业大学 湖南省兽药工程技术研究中心,长沙 410000;中国农业科学院上海兽医研究所 农业部兽用化学药物及制剂学重点实验室,上海,200241;[薛飞群; 郭春娜; 杨帆帆; 王霄旸; 张可煜] 中国农业科学院上海兽医研究所;[易远胜; 孙志良] 湖南农业大学
关键词:
抗球虫药;沙咪珠利;高效液相色谱;鸡盲肠
摘要:
为研究抗球虫新化合物沙咪珠利在鸡盲肠中的药物代谢和消除情况,建立检测鸡盲肠中沙咪珠利的高效液相色谱法.检测方法以妥曲珠利亚砜为内标,X TERRA MS C185μm(Waters,4.6×250 mm)柱为分析柱,乙腈:0.2%磷酸水溶液(v/v,35:65)为流动相等度洗脱,C18柱分离后,在251 nm波长紫外检测器检测,内标法定量.鸡盲肠样品经匀浆化后,乙腈提取、己烷除脂.结果表明,该方法在0.2~40 mg/kg添加浓度范围内检测鸡盲肠中沙咪珠利具有很好的线性关系(r2=0.994);平均相对回收率为90%~107%,日内变异系数2.37%~8.72%;日间变异系数4.09%~6.80%,具有特异性好、灵敏度高、准确性和重现性良好、操作简单等优点,能满足鸡盲肠中沙咪珠利的检测需求.
语种:
中文
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钩吻提取物对生长猪生长性能、肠道形态和盲肠菌群的影响
作者:
陈小军;王宇;王帅帅;伍勇;李元义;...
期刊:
动物营养学报 ,2018年30(9):3626-3633 ISSN:1006-267X
作者机构:
湖南农业大学动物医学院, 长沙, 410128;湖南省兽药工程技术研究中心, 湖南省兽药工程技术研究中心, 长沙, 410128;加拿大麦吉尔大学健康研究中心, 蒙特利尔, H3G 1A4;[陈小军; 王宇; 王帅帅; 孙志良] 湖南农业大学动物医学院, 长沙, 410128;[伍勇] 湖南省兽药工程技术研究中心, 湖南省兽药工程技术研究中心, 长沙, 410128
关键词:
钩吻提取物;生长猪;生长性能;肠道形态;盲肠菌群
摘要:
本试验旨在研究钩吻提取物对生长猪生长性能、肠道形态和盲肠菌群的影响。选取56日龄生长猪75头,随机分为5组,每组3个重复,每个重复5头。对照组饲喂基础饲粮,钩吻醇提物组分别饲喂在基础饲粮中添加25、50、100 mg /kg钩吻醇提物的试验饲粮,钩吻酸提物组饲喂在基础饲粮中添加150 mg /kg钩吻酸提物的试验饲粮,试验期49 d。结果显示:与对照组相比,饲粮添加钩吻提取物对生长猪的平均日采食量均无显著影响(P>0.05),显著提高了平均日增重(P<0.05),显著降低了料重比(P<0.05),以添加100 mg /kg钩吻醇提物效果最佳;与对照组相比,饲粮添加100 mg /kg钩吻醇提物显著增加了空肠、回肠绒毛高度(P<0.05),显著降低了十二指肠隐窝深度(P<0.05);透射电镜观察结果显示,饲粮添加钩吻提取物使空肠组织微绒毛更加均匀、整齐、致密,相邻细胞间的紧密连接、中间连接和桥粒等结构均结构清晰、完整;与对照组相比,饲粮添加50、100 mg /kg钩吻醇提物显著降低了盲肠中大肠杆菌的数量(P<0.05),显著增加了盲肠中乳酸杆菌的数量(P<0.05) 。由此可见,钩吻醇提物和钩吻酸提物均可改善生长猪肠道形态,调整盲肠菌群结构,提高生长猪的平均日增重,降低料重比,以100 mg /kg钩吻醇提物效果最佳。
语种:
中文
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Elevated Levels of ADMA Are Associated with Lower DDAH2 and Higher PRMT1 in LPS-Induced Endometritis Rats
作者:
Xiao, Hong-Bo* ;Sui, Guo-Guang;Lu, Xiang-Yang;Sun, Zhi-Liang
期刊:
Inflammation ,2018年41(1):299-306 ISSN:0360-3997
通讯作者:
Xiao, Hong-Bo
作者机构:
[Xiao, Hong-Bo; Sui, Guo-Guang] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Lu, Xiang-Yang] Hunan Agr Univ, Hunan Prov Univ Key Lab Agr Biochem & Biotransfor, Changsha 410128, Hunan, Peoples R China.;[Lu, Xiang-Yang] Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha 410128, Hunan, Peoples R China.;[Sun, Zhi-Liang] Hunan Agr Univ, Biol Vet Drugs Branch, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Hunan, Peoples R China.
通讯机构:
[Xiao, Hong-Bo] H;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
关键词:
asymmetric dimethylarginine;dimethylarginine dimethylaminohydrolase 2;protein-methyl transferase 1;endometritis;rat
摘要:
Chronic endometritis is a continuous inflammation of uterine endometrium. Recent research has shown that higher asymmetric dimethylarginine (ADMA) levels contribute to endothelial dysfunction. In the present study, we tested whether there is a correlation between endometritis and ADMA in LPS-induced endometritis rat and the mechanisms involved. Thirty-six rats were divided into two groups: blank control group and rat model of endometritis group. The entire infused uterus were removed to observe the changes of histopathology, production of myeloperoxidase (MPO), tumor necrosis factor-α (TNF-α), interleukin (IL)-6, 8-isoprostane, and reactive oxygen species (ROS), and gene expression of dimethylarginine dimethylaminohydrolase 2 (DDAH2), protein-methyl transferase 1 (PRMT1), TNF-α, and IL-6. In endometritis rat group, characteristic histopathologic changes in uteri were observed. The uterine 8-isoprostane, ROS, MPO activity, IL-6 and TNF-α concentrations, PRMT1, IL-6, and TNF-α expressions were significantly elevated, and DDAH2 expression was notably reduced in endometritis group compared with control group. The present findings suggest that elevated levels of ADMA are associated with lower DDAH2 and higher PRMT1 in LPS-induced endometritis rat. © 2017, Springer Science+Business Media, LLC.
语种:
英文
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Identification of gelsemine metabolites in rat liver S9 by high-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry
作者:
Yang, Kun;Huang, Ya-Jun;Xiao, Sa;Liu, Yan-Chun;Sun, Zhi-Liang;...
期刊:
Rapid Communications in Mass Spectrometry ,2018年32(1):19-22 ISSN:0951-4198
通讯作者:
Liu, Zhao-Ying
作者机构:
[Sun, Zhi-Liang; Yang, Kun; Liu, Zhao-Ying; Xiao, Sa; Huang, Ya-Jun] Hunan Agr Univ, Coll Vet Med, Hunan Engn Res Ctr Vet Drug, Changsha 410128, Hunan, Peoples R China.;[Tang, Qi; Liu, Zhao-Ying; Liu, Yi-Song; Liu, Yan-Chun] Hunan Agr Univ, Natl & Local Union Engn Res Ctr Vet Herbal Med Re, Changsha 410128, Hunan, Peoples R China.;[Sun, Zhi-Liang; Liu, Zhao-Ying] Hunan Agr Univ, Coll Vet Med, Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhao-Ying] Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.
通讯机构:
[Liu, Zhao-Ying] H;Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.
摘要:
Rationale: Gelsemine has been extensively studied because of its anti-tumor, immunomodulatory, insecticidal itching and other significant effects. However, limited information on the pharmacokinetics and metabolism of gelsemine has been reported. Therefore, the purpose of the present study was to investigate the in vitro metabolism of gelsemine in rat liver S9 by using rapid and accurate high-performance liquid chromatography/ quadrupole-time-of-flight mass spectrometry (HPLC/QqTOF-MS). Methods: The incubation mixture was processed with 15% trichloroacetic acid. Multiple scans of gelsemine metabolites and accurate mass measurements were automatically performed simultaneously through data-dependent acquisition in only 30 min. The structural elucidations of these metabolites were performed by comparing their changes in accurate molecular masses and product ions with those of the parent drug. Results: Five metabolites of gelsemine were identified in rat liver S9. Of these, four metabolites of gelsemine were identified for the first time. The present results showed that the metabolic pathways of gelsemine are oxidation, demethylation, and dehydrogenation in rat liver S9. Conclusions: In this study, metabolites of gelsemine in liver S9 were identified and elucidated firstly using the HPLC/QqTOF-MS method. The proposed metabolic pathways of gelsemine in liver S9 will provide a basis for further studies of the in vivo metabolism of gelsemine in animals and humans. Copyright © 2017 John Wiley & Sons, Ltd.
语种:
英文
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