摘要：
The objective was to investigate the effects of a novel DNA vaccine (pcISI) harboring two copies of inhibin alpha (1-32) fragments on immune response, hormone concentrations and reproductive performance in rats. Female Wistar rats (n = 18 per group) were immunized (twice, 4 wk apart) with 10, 50, or 100 mu g (T-1, T-2 and T-3, respectively), of the pcISI plasmid. At 4 wk after the second immunization, plasma antibody titers were higher (P < 0.05) in T-3 than in either T-1 or T-2 (0.341 +/- 0.123, 0.236 +/- 0.068, and 0.251 +/- 0.077, respectively, mean +/- SD). Concurrrently, plasma concentrations of FSH and estradiol were highest (P < 0.05) in T-3, and were higher (P < 0.05) in T-1 and T-2 than in control groups. For antibody-positive rats, there was a correlation (P < 0.01) between antibody titer and FSH concentrations after two pcISI immunizations. The number of mature follicles in the T-3 group (46.00 +/- 4.65) was higher (P < 0.05) than in two control groups (29.25 +/- 3.72 and 27.92 +/- 3.48), and also higher (P < 0.05) than in T-1 and T-2 (37.17 +/- 4.99 and 38.75 +/- 7.09). Antibody-positive rats had more mature ovarian follicles than negative rats (46.75 +/- 4.23 vs. 35.60 +/- 3.38, P < 0.05). Moreover, litter size and number of placentas were increased (P < 0.05) in the pcISI immunization groups, except for the T-1 group, compared to the control groups. In conclusion, the pcISI DNA vaccine successfully induced a humoral immune response, improved reproductive hormone concentrations, stimulated follicular development, and increased number of placentas and litter size. Furthermore, 100 mu g yielded the best immune response. (c) 2012 Elsevier Inc. All rights reserved.
摘要：
雄性特异性组织相容性抗原（male specific minor histompatibility antigens,H-Y抗原）是由Y染色体上的基因编码,在雄性动物细胞中普遍表达（包括胚胎和滋养层细胞）。H-Y抗原不仅能引起基因型相同的雌性动物排斥雄性组织,也能导致人白细胞抗原匹配干细胞移植术后出现移植抗宿主性疾病（GVH）。细胞毒性T淋巴细胞检测到几个不同的H-Y抗原表位,这些肽是从胞内蛋白分离出来,由主要组织相容性复合分子结合呈递在细胞表面。H-Y抗原肽与人白细胞抗原Ⅰ类和Ⅱ类分子特异结合参与免疫反应,从而影响移植结果。近年来越来越多的文献报道了H-Y抗原的相关研究,作者主要综述编码H-Y抗原的相关候选基因,并对它在疾病等方面的前景作出了一些展望。
通讯机构：
[Xue, Li-Qun] H;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
关键词：
Mouse model;PCV-2b;Histopathological lesions;Viral distribution
摘要：
The viral distribution and lesions in Kunming mice experimentally infected with porcine circovirus type 2b (PCV-2b) were investigated. Seventy special pathogen free mice were divided into 2 groups with 35 mice in each group. The test group (TG) was infected with PCV-2b, the control group (CG) was inoculated with sterile cell cultures. Five mice in each group were sacrificed at 3, 7, 14, 21, 28, 35 and 42 dpi (day post infection), respectively. Necropsies were performed on all mice and tissues were collected for testing by histopathology, immunohistochemistry, transmission electron microscope (TEM) and polymerase chain reaction (PCR). Apoptosis and necrosis in lymphoid organs were observed in virus-infected mice, and became severe from 14 to 28 dpi. The proportion of PCV-2b antigen-positive cells was moderate in lung, heart, thymus, liver or kidney, and low in brain from TG. In spleen and cervical lymph node, the proportions of PCV-2b antigen-positive cells were low to high from 7 to 28 dpi, and moderate from 35 to 42 dpi. PCV-2b DNA was detected in all tissues examined in TG from 7 to 42 dpi. Viral inclusion bodies presented in the cytoplasm of lymphocytes, macrophages, hepatocytes, podocytes, neurocytes, spermatids and uterine epithelial cells in TG. In CG, no viruses and viral lesions were detected. PCV-2b could replicate in mice, and PCV-2b associated lesions in mice were similar to those observed in pigs. The present results indicate that it is possible to use Kunming mouse as an animal model for PMWS research.
通讯机构：
[Yuan, Hui] H;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
关键词：
DNA damage;Gossypol;Oxidase;Sertoli cells
摘要：
The study was designated to explore the toxic effects of gossypol on piglet sertoli cells. Sertoli cellswere isolated from piglet testes using a two-step enzyme digestion and followed by differential plating.Piglet sertoli cells were cultured and classified into five groups, that is, group A, the control withoutgossypol, group B with 2.5 μg/ml gossypol, group C with 5 μg/ml gossypol, group D with 10 μg/mlgossypol and group E with 20 μg/ml gossypol. We found that sertoli cells’ growth was inhibited bygossypol at dose 2.5 μg/ml when compared with the control group. The oxidase activity of sertoli cellalso decreased at 2.5 μg/m gossypol. Moreover, DNA damage of sertoli cells was observed at 5 μg/mlgossypol. Putting this into consideration, our study suggests that exposure of gossypol to sertoli cellsleads to an inhibition of sertoli cell growth and oxidase activity of sertoli cells at a low concentration,whereas gossypol results in DNA damage of sertoli cells at a higher concentration.Keywords: Gossypol, sertoli cells, oxidase, DNA damage
