A MYB transcription factor, BnMYB2, cloned from ramie (Boehmeria nivea) is involved in cadmium tolerance and accumulation
作者：
Zhu, Shoujing;Shi, Wenjuan;Jie, Yucheng* ;Zhou, Qingming;Song, Chenbo
期刊：
PLOS ONE ,2020年15(5):e0233375 ISSN：1932-6203
通讯作者：
Jie, Yucheng
作者机构：
[Zhou, Qingming; Zhu, Shoujing; Jie, Yucheng] Hunan Agr Univ, Inst Ramie, Changsha, Hunan, Peoples R China.;[Shi, Wenjuan; Song, Chenbo; Zhu, Shoujing] Yichun Univ, Coll Life Sci Resources & Environm, Yichun, Jiangxi, Peoples R China.
通讯机构：
[Jie, Yucheng] H;Hunan Agr Univ, Inst Ramie, Changsha, Hunan, Peoples R China.
摘要：
MYB-related transcription factors play important roles in plant development and response to various environmental stresses. In the present study, a novel MYB gene, designated as BnMYB2 (GenBank accession number: MF741319.1), was isolated from Boehmeria nivea using rapid amplification of cDNA ends (RACE) and RT-PCR on a sequence fragment from a ramie transcriptome. BnMYB2 has a 945 bp open reading frame encoding a 314 amino acid protein that contains a DNA-binding domain and shares high sequence identity with MYB proteins from other plant species. The BnMYB2 promoter contains several putative cis-acting elements involved in stress or phytohormone responses. A translational fusion of BnMYB2 with enhanced green fluorescent protein (eGFP) showed nuclear and cytosolic subcellular localization. Real-time PCR results indicated that BnMYB2 expression was induced by Cadmium (Cd) stress. Overexpression of BnMYB2 in Arabidopsis thaliana resulted in a significant increase of Cd tolerance and accumulation. Thus, BnMYB2 positively regulated Cd tolerance and accumulation in Arabidopsis, and could be used to enhance the efficiency of Cd removal with plants.
语种：
英文
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河东乌麦低分子量谷蛋白基因的生物信息学分析
作者：
李倩;李密;揭雨成;杨友伟
期刊：
山东化工 ,2019年48(2):28-32 ISSN：1008-021X
作者机构：
湖南农业大学 农学院,湖南 长沙 410128;桃源县第三中学,湖南 桃源 415701;湖南文理学院 生命与环境科学学院,湖南 常德,415000;湖南农业大学 农学院,湖南 长沙,410128;[杨友伟; 李密] 湖南文理学院
关键词：
河东乌麦;低分子量谷蛋白;生物信息学
摘要：
低分子量谷蛋白是一种重要的贮藏蛋白质。它对小麦的加工品质有重大影响,尤其在面点制作过程中具有较大作用。本研究通过提取纯化河东乌麦总基因组DNA,利用特异引物进行扩增,扩增产物经回收纯化后送生物公司测序。生物信息学软件分析发现,得到1条长度为918bp的低分子量谷蛋白编码基因序列,其上共有18个酶切位点,GC含量为49.0%。该基因与来自于普通小麦低分子量麦谷蛋白得亚基GluA3-21和GluA3-1基因相似值达到99%。该基因编码306个氨基酸,分子量大小为34588.0kDa,信号肽序列为MKTFLVFALLAVVATSAIA。Gln谷氨酰胺含量最高,为35.00%,二级结构主要为无规则卷曲结构组成,其次为片层结构。本研究结果可为低分子量谷蛋白与小麦面粉加工品质关系提供参考。
语种：
中文
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流式细胞术测定苎麻基因组大小
作者：
何鑫玺;钟启;钟英丽;揭雨成
期刊：
分子植物育种 ,2019年17(1):99-103 ISSN：1672-416X
作者机构：
[钟启; 何鑫玺; 钟英丽] 湖南农业大学生物科学技术学院,长沙,410000;湖南农业大学农学院,长沙,410000;[揭雨成] 湖南农业大学
关键词：
苎麻(Boehmeria nivea);基因组大小;流式细胞术;水稻
摘要：
苎麻属于荨麻科苎麻属,是目前非常重要的纺织原料,有着广泛的开发前景。为了更好地研究苎麻基因组信息,拟通过流式细胞术测定苎麻基因组大小。本研究以水稻(Oryza sativa)日本晴为内标,湘苎3号为待测样品,分别分离水稻和苎麻叶片细胞的细胞核,PI染色,用流式细胞仪对水稻和苎麻样品的PI发射荧光强度进行测定。通过比较水稻和苎麻样品的荧光强度的倍数关系,计算得到苎麻基因组大小,并采用外标法和内标法同时进行测定和计算。以水稻日本晴为外标,苎麻3号为待测样品,测得苎麻基因组大小为332.5 Mb,而用内标法得到的苎麻基因组大小为300.5 Mb,相比之下内标法测定的变异系数更低,更为准确。苎麻基因组大小的测定为苎麻基因组文库的建立及其基因组相关研究提供了理论指导。
语种：
中文
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Characterization of cadmium concentration and translocation among ramie cultivars as affected by zinc and iron deficiency
作者：
She, Wei;Cui, Guoxian* ;Li, Xueling;Su, Xiaohui;Jie, Yucheng;...
期刊：
ACTA PHYSIOLOGIAE PLANTARUM ,2018年40(6):1-11 ISSN：0137-5881
通讯作者：
Cui, Guoxian
作者机构：
[Yang, Ruifang; Cui, Guoxian; Li, Xueling; She, Wei; Su, Xiaohui; Jie, Yucheng] Hunan Agr Univ, Ramie Res Inst, Changsha 410128, Hunan, Peoples R China.;[Yang, Ruifang; Cui, Guoxian; Li, Xueling; She, Wei; Su, Xiaohui; Jie, Yucheng] Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Cui, Guoxian] H;Hunan Agr Univ, Ramie Res Inst, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.
关键词：
Cadmium accumulation;Translocation;Ramie;Fe deficiency;Zn deficiency
摘要：
Zn and Fe are essential nutritional elements in plants and play important roles in various physiological processes of plants. Zn and Fe are chemically similar to cadmium (Cd); therefore, Zn and Fe may mediate Cd-induced physiological or metabolic changes in plants. In order to evaluate the interaction between Cd, Zn and Fe, we conducted a hydroponics experiment to determine the plant biomass, photosynthetic characteristics, and Cd accumulation of ten ramie cultivars under Zn/Fe-sufficient or Zn/Fe-deficient conditions in the presence of 32 mu M CdCl2. Ramie varied among cultivars in morpho-physiological response to Cd stress as well as Cd accumulation, translocation and distribution. Zn and Fe deficiency increased the concentration and amount of Cd in plant organs, but decreased TFstem to leaf and TFroot to stem. Cultivars with more Cd in roots and shoots showed smaller increase in Cd accumulation under Zn and Fe-deficiency stress. Xiangzhu 7 and Duobeiti 1 showed a higher capacity of Cd accumulation in their shoots. Zn and Fe deficiency decreased Pn, but increased Ci, Gs, and E in most cultivars. The difference in Cd translocation among ramie cultivars was mainly ascribed to the difference in plant transpiration.
语种：
英文
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饲用苎麻施肥技术与收获方式研究
作者：
王丹;黎淸燮;张英;揭红东;班琦沁;...
期刊：
南方农业 ,2018年12(30):39-41 ISSN：1673-890X
作者机构：
湖南农业大学农学院,湖南长沙,410128;[揭红东; 黎淸燮; 揭雨成; 班琦沁; 王丹; 张英] 湖南农业大学
关键词：
饲用苎麻;施肥;营养品质;收获方式
摘要：
开展饲用苎麻施肥技术和收获方式研究,筛选出科学合理的饲用苎麻施肥技术与收获方式。结果表明:冬肥每667 m2施复合肥30 kg,每次刈割后每667 m2追施尿素4 kg施肥处理产量最高,每667 m2鲜重产量达到8 519.81 kg,粗蛋白含量达到21.07%;小型自走式收割机收获方式效率是背负式小型割草机收获方式5.34倍,是人工收割方式的13倍。
语种：
中文
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不同干燥方式对饲用苎麻营养品质的影响
作者：
班琦沁;张维童;王贤芳;张英;揭红东;...
期刊：
南方农业 ,2018年12(30):191-193 ISSN：1673-890X
作者机构：
湖南农业大学农学院,湖南长沙,410128;[揭红东; 张维童; 揭雨成; 王贤芳; 班琦沁; 王丹; 张英] 湖南农业大学
关键词：
苎麻;草粉;干燥方式;营养品质
摘要：
为明确不同干燥方式对饲用苎麻营养品质的影响,开展了对不同干燥方式下对饲用苎麻营养品质影响的研究。结果表明:烘干法和压杆晒干法对饲用苎麻的营养品质影响最小,水泥地晒干法和阴干法对饲用苎麻的营养品质影响最大。所以烘干法和压杆晒干法是最佳的干燥方式,水泥地晒干法和阴干法是最差的干燥方式。
语种：
中文
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草牧业发展新形势下南方饲草学课程体系教学改革研究
作者：
张志飞;胡龙兴;陈桂华;曾宁波;揭雨成
期刊：
教育教学论坛 ,2018年(38):135-136 ISSN：1674-9324
作者机构：
湖南农业大学农学院,湖南 长沙,410128;[陈桂华; 曾宁波; 揭雨成; 张志飞; 胡龙兴] 湖南农业大学
关键词：
饲草学;课程体系;教学改革;草牧业
摘要：
饲草学是草业科学专业重要方向。基于草牧业发展新形势以及草牧业领域科技人才短缺的现状,湖南农业大学从教学理念、课程设置、教学方式方法、教学手段、教学管理体制、课程评价体系等方面系统实施饲草学课程体系的教学改革的措施。
语种：
中文
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洞庭湖区稻虾共作模式研究
作者：
钟斌;金红春;揭雨成;杨春浩
期刊：
湖南农业科学 ,2017年(10):60-62 ISSN：1006-060X
作者机构：
安乡县水产技术推广站;[金红春] 安乡县科技和工信局;湖南农业大学农学院;[杨春浩] 安乡县发展和改革局;[揭雨成] 湖南农业大学
关键词：
洞庭湖区;低洼湖田;稻虾连共作;防灾减灾;循环农业
摘要：
针对洞庭湖区低洼湖田地势低洼,只能种植一季水稻,土地利用率不高的情况,通过改造低洼湖田,研究了稻虾共作的生态种养模式及效益.结果表明：低洼田一季稻的利润约为 575 元 /667m^2,稻虾共作试验田利润约为 1 661 元 /667m^2;稻虾共作使大部分低洼荒废湖田得到充分利用,防灾减灾、生态效果明显,不仅提高了粮食安全水平,还增加了单位面积产值,单位利润是-季稻利润的2.9 倍.稻虾共作模式是一种互惠互利的立体循环农业,适合在洞庭湖区推广.
语种：
中文
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不同纤维细度苎麻种质镉富集与转运和积累能力比较
作者：
符慧琴;揭红东;揭雨成;邢虎成;温圣贤
期刊：
湖南农业大学学报(自然科学版) ,2017年43(4):359-365 ISSN：1007-1032
作者机构：
湖南农业大学苎麻研究所, 湖南, 长沙, 410128;湖南农业大学园艺园林学院, 湖南, 长沙, 410128;湖南农业大学农学院, 湖南, 长沙, 410128;[符慧琴; 揭雨成; 邢虎成] 湖南农业大学苎麻研究所, 湖南, 长沙, 410128;[揭红东] 湖南农业大学园艺园林学院, 湖南, 长沙, 410128
关键词：
苎麻;纤维细度;镉富集;镉吸收;镉积累
摘要：
为探明镉在不同细度苎麻种质体内的积累与富集性差异,对20个苎麻种质进行镉污染大田试验。结果表明:供试苎麻种质镉富集系数的变幅为0.97~1.58,富集系数较高的分别是中细度种质‘新1号’‘中苎1号’和高细度种质‘湘饲纤兼用苎麻1号’,富集系数依次为1.58、1.55、1.54,属于镉高富集苎麻种质,镉富集系数(0.97)最小的是高细度种质‘黄平青麻’;供试苎麻种质镉转运系数的变幅为0.66~1.41,转运系数最高的是中细度种质‘耒阳青麻’,其转运系数为1.41;供试苎麻种质年镉积累量的变幅为10.24 ~21.87 g/hm~2,筛选出高积累镉苎麻种质‘C’ ‘中苎1号’和‘多倍体1号’,其年镉积累量分别为21.87、19.87、18.75 g/hm~2。综合分析,苎麻纤维细度不影响其镉富集能力。
语种：
中文
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镉胁迫下不同细度品种苎麻耐性比较研究
作者：
符慧琴;揭红东;马玉申;揭雨成;邢虎成;...
期刊：
中国农学通报 ,2017年33(15):1-9 ISSN：1000-6850
作者机构：
湖南农业大学苎麻研究所,长沙 410128;湖南农业大学农学院,长沙 410128;湖南农业大学园艺园林学院,长沙,410128;湖南农业大学农学院,长沙,410128
关键词：
苎麻;品种;纤维细度;耐Cd能力;阈值
摘要：
旨在了解不同细度品种对重金属Cd耐性的差异性.试验选择长势一致苎麻扦插苗(苗龄60天)进行移栽,土壤Cd胁迫处理分别为0、25、75、150 mg/kg共4个处理,进行为期一整年的Cd耐性试验调查.结果表明:12个不同细度品种苎麻在25 mg/kg土壤Cd胁迫处理下的综合耐性指数在0.80～1.18区间,耐性最好的是中细度品种‘中苎一号’,最差的是低细度品种‘大竹黄白麻’;在75 mg/kg胁迫处理下的综合耐性指数在0.09～0.77之间,表现最好的是中细度品种‘湘苎3号’,最差的是低细度品种‘大竹黄白麻’.在150 mg/kg土壤Cd胁迫处理下的耐性指数在0.32～0.54之间,最好的是高细度品种‘厚皮种一号’,最差的是低细度品种‘大竹黄白麻’.由此可知,苎麻对重金属Cd具有很好的耐受能力,且呈现出中、高细度品种的Cd耐性大于低细度品种.75 mg/kg Cd胁迫浓度是待测品种‘大竹黄白麻’、‘宁乡冲天炮’、‘浏阳大叶青’、‘邻水青顶家麻’、‘川苎八号’、‘中苎一号’、‘武岗红皮麻’、‘湘饲纤兼用一号’苎麻的耐Cd阈值.150 mg/kg Cd胁迫浓度是待测品种‘湘苎三号’、‘多倍体一号’、‘资兴麻’、‘厚皮种一号’的耐Cd阈值.
语种：
中文
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A Ramie bZIP Transcription Factor BnbZIP2 Is Involved in Drought, Salt, and Heavy Metal Stress Response
作者：
Huang, Chengjian;Zhou, Jinghua;Jie, Yucheng* ;Xing, Hucheng;Zhong, Yingli;...
期刊：
DNA AND CELL BIOLOGY ,2016年35(12):776-786 ISSN：1044-5498
通讯作者：
Jie, Yucheng
作者机构：
[Huang, Chengjian; Zhou, Jinghua; Xing, Hucheng; Liu, Zehang; Ma, Yushen; Yu, Weilin; She, Wei; Zhang, Ying; Jie, Yucheng] Hunan Agr Univ, Inst Ramie, Changsha 410128, Hunan, Peoples R China.;[Huang, Chengjian] Dazhou Inst Agr Sci, Dazhou, Peoples R China.;[Zhou, Jinghua; Xing, Hucheng; Liu, Zehang; Ma, Yushen; She, Wei; Zhang, Ying; Jie, Yucheng] Hunan Agr Univ, Coll Agron, Changsha, Hunan, Peoples R China.;[Xing, Hucheng; Ma, Yushen; Zhong, Yingli; Yu, Weilin; Zhang, Ying; Jie, Yucheng] Hunan Prov Crop Germplasm Innovat & Utilizat Lab, Changsha, Hunan, Peoples R China.;[Zhong, Yingli] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China.
通讯机构：
[Jie, Yucheng] H;Hunan Agr Univ, Inst Ramie, Changsha 410128, Hunan, Peoples R China.
关键词：
Boehmeria nivea;abiotic stress;bZIP;promoter;transgenic plant
摘要：
bZIP transcription factors play key roles in plant growth, development, and stress signaling. A bZIP gene BnbZIP2 (GenBank accession number: KP642148) was cloned from ramie. BnbZIP2 has a 1416 base pair open reading frame, encoding a 471 amino acid protein containing a characteristic bZIP domain and a leucine zipper. BnbZIP2 shares high sequence similarity with bZIP factors from other plants. The BnbZIP2 protein is localized to both nuclei and cytoplasm. Transcripts of BnbZIP2 were found in various tissues in ramie, with significantly higher levels in female and male flowers. Its expression was induced by drought, high salinity, and abscisic acid treatments. Analysis of the cis-elements in promoters of BnbZIP2 identified cis-acting elements involved in growth, developmental processes, and a variety of stress responses. Transgenic Arabidopsis plants' overexpression of BnbZIP2 exhibited more sensitivity to drought and heavy metal Cd stress during seed germination, whereas more tolerance to high-salinity stress than the wild type during both seed germination and plant development. Thus, BnbZIP2 may act as a positive regulator in plants' response to high-salinity stress and be an important candidate gene for molecular breeding of salt-tolerant plants.
语种：
英文
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洞庭湖区低洼地夏季玉米品种比较
作者：
杨瑞芳;揭雨成;佘玮;邢虎成;陈小倩;...
期刊：
湖南农业科学 ,2016年(10):21-23 ISSN：1006-060X
作者机构：
湖南农业大学苎麻研究所，湖南长沙 410128;湖南省种质资源创新与资源利用重点实验室，湖南长沙 410128;湖南农业大学苎麻研究所,湖南长沙,410128;安乡县安全乡农技站,湖南安乡,415612;[揭雨成; 邢虎成; 陈小倩; 杨瑞芳; 佘玮] 湖南农业大学
关键词：
夏季玉米;品种;洞庭湖区;低洼地
摘要：
为了筛选适合洞庭湖区种植的优良玉米品种，2015年在湖南省安乡县平原湖区进行了不同水位旱地玉米品种比较试验。结果表明：10个参试品种中正常地和低洼地产量最高的都是新中玉1号，分别为789.41和691.00 kg/667 m2，而且综合性状和田间表现较好，适宜在环洞庭湖平原湖区推广应用。
语种：
中文
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A ramie (Boehmeria nivea) bZIP transcription factor BnbZIP3 positively regulates drought, salinity and heavy metal tolerance
作者：
Huang, Chengjian;Zhou, Jinghua;Jie, Yucheng* ;Xing, Hucheng;Zhong, Yingli;...
期刊：
Molecular Breeding ,2016年36(8):1-15 ISSN：1380-3743
通讯作者：
Jie, Yucheng
作者机构：
[Huang, Chengjian; Zhou, Jinghua; Xing, Hucheng; Ma, Yushen; Zhong, Yingli; Yu, Weilin; She, Wei; Jie, Yucheng] Hunan Agr Univ, Inst Ramie, Changsha 410128, Hunan, Peoples R China.;[Huang, Chengjian; Wei, Gang] Dazhou Inst Agr Sci, Dazhou 635000, Peoples R China.;[Zhou, Jinghua; Xing, Hucheng; Ma, Yushen; Yu, Weilin; Jie, Yucheng] Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.;[Xing, Hucheng; Zhong, Yingli; She, Wei; Jie, Yucheng] Hunan Prov Crop Germplasm Innovat & Utilizat Lab, Changsha 410128, Hunan, Peoples R China.;[Zhong, Yingli] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Jie, Yucheng] H;Hunan Agr Univ, Inst Ramie, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.;Hunan Prov Crop Germplasm Innovat & Utilizat Lab, Changsha 410128, Hunan, Peoples R China.
关键词：
Cloning;Crops;Cultivation;Germination;Heavy metals;Physiological models;Plants (botany);Polymerase chain reaction;Proteins;Transcription;Abiotic stress;Adaptive response;Boehmerium nivea;BZIP;bZIP transcription factors;Heavy metal tolerance;Physiological process;Process response;Promoter;Rapid amplification of cDNA ends;Drought
摘要：
bZIP genes play important roles in the regulation of many developmental and physiological processes and adaptive responses to the environment in plants. In the present study, a novel bZIP gene, designated as BnbZIP3 (Genbank accession number: KP462877), was cloned from ramie plants based on the sequence of Unigene6582 fragment in ramie transcriptome using rapid amplification of cDNA ends (RACE) technique and real-time PCR. Results indicated that BnbZIP3 had a 999-bp open reading frame, encoding a 332 amino acid protein that containing a characteristic bZIP domain, and shared high sequence identities with bZIP factors from other plants. Translational fusion of BnbZIP3 with EGFP showed subcellular localization of the protein in nucleus. Transcripts of BnbZIP3 were found in different tissues of ramie plants. Expression of BnbZIP3 was induced by drought, high salinity and ABA treatments. The promoter of BnbZIP3 contained many cis-acting elements involved in multiple stress responses. Overexpression of BnbZIP3 in transgenic Arabidopsis plants inhibited the growth of roots under normal growth conditions, whereas it improved the growth of roots under dehydration, salinity and heavy metal Cd stress, and increased germination of seeds under drought conditions. Thus, BnbZIP3 may positively regulate stress tolerance and be used to engineer enhanced drought, salt and heavy metal tolerance of ramie varieties or other industrial crops. © 2016, Springer Science+Business Media Dordrecht.
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英文
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不同葛种质资源的植物学性状、藤蔓产量和营养品质分析
作者：
谭燕群;陈建芳;揭雨成;邢虎成;张英;...
期刊：
湖南林业科技 ,2016年43(5):85-87+91 ISSN：1003-5710
作者机构：
湖南农业大学苎麻研究所;湖南农业大学计财处;[熊力夫] 长沙市葛根工程技术研究中心;[陈建芳; 谭燕群; 揭雨成; 邢虎成; 张英] 湖南农业大学
关键词：
葛种质;植物学性状;藤蔓;产量;营养成分
摘要：
对8个葛种质的植物学性状、营养成分和产量进行研究,以评价葛根饲用价值。结果表明：桃江粉葛与湘葛2号葛蔓较长,江西野葛和桃江粉葛的叶较大。湘葛3号、桃江野葛1和桃江野葛2的茎皮色为褐色,其它5种为黄褐色。江西粉葛、湘葛3号和桃江野葛1的叶形是近圆形,江西野葛和桃江粉葛是卵圆形,其它3种是菱形。湘葛2号和桃江粉葛的叶面皱纹较少。8个葛种质的叶柄色全是绿色。葛根中粗纤维的含量最高,其次是粗蛋白、灰分、粗脂肪、钙、磷。其中,湘葛2号中粗纤维和钙的含量较高,桃江野葛1的粗蛋白含量较高,湘葛3号的灰分和粗脂肪含量较高,桃江粉葛的磷含量较高。产量最高的是江西野葛。
语种：
中文
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Effect of shade on plant traits, gas exchange and chlorophyll content in four ramie cultivars
作者：
Huang, C. -J.;Wei, G.;Jie, Y. -C.* ;Xu, J. -J.;Anjum, S. A.;...
期刊：
PHOTOSYNTHETICA ,2016年54(3):390-395 ISSN：0300-3604
通讯作者：
Jie, Y. -C.
作者机构：
[Jie, Y. -C.; Huang, C. -J.] Hunan Agr Univ, Inst Ramie, Changsha, Hunan, Peoples R China.;[Wei, G.; Xu, J. -J.; Huang, C. -J.] Dazhou Inst Agr Sci, Dazhou, Sichuan, Peoples R China.;[Anjum, S. A.] Southwest Univ, Coll Agron & Biotechnol, Chongqing, Peoples R China.;[Tanveer, M.] Univ Agr Faisalabad, Dept Agron, Faisalabad 38040, Pakistan.
通讯机构：
[Jie, Y. -C.] H;Hunan Agr Univ, Inst Ramie, Changsha, Hunan, Peoples R China.
关键词：
abiotic stress;Boehmeria nivea;photosynthesis;pigments
摘要：
The objective of this study was to investigate a response to low-light environments in hybrids and commercial cultivars of Boehmeria nivea L. Two hybrids (Chuanzhu 11 and Chuanzhu 8) and two commercial cultivars (Chuanzhu 12 and Chuanzhu 6) of ramie were subjected to a shade treatment for 6, 12, and 18 days. The shade treatment led to a significant decrease in some plant traits and fiber yield in four ramie cultivars, whereas their leaf area and plant height increased. In addition, net photosynthesis and stomatal conductance significantly declined in response to shade, while transpiration rate and intercellular CO2 did not significantly change. Moreover, chlorophyll (Chl) and carotenoid (Car) concentration, Chl/Car, and Chl (a+b) per leaf dry mass significantly increased in the response to shade, while the Chl a/b ratio decreased. Furthermore, Chuanzhu 6 and Chuanzhu 11 were more tolerant to shade than Chuanzhu 12 and Chuanzhu 8, thus, they could be potentially used for management practices and breeding programs. © 2016, The Institute of Experimental Botany.
语种：
英文
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湖南坡耕地皇竹草＋黑麦草周年供青模式研究
作者：
邢虎成;张跃乾;揭雨成;张英
期刊：
安徽农业科学 ,2016年44(24):98-101 ISSN：0517-6611
作者机构：
湖南农业大学苎麻研究所，湖南长沙410128;湖南省种质资源创新与资源利用重点实验室，湖南长沙410128;湖南农业大学苎麻研究所,湖南长沙,410128;[揭雨成; 张跃乾; 邢虎成; 张英] 湖南农业大学
关键词：
南方坡耕地;皇竹草;黑麦草;周年供应;留种
摘要：
[目的]以红壤坡耕地为研究对象，探讨玉米／皇竹草＋黑麦草周年供草模式。[方法]分别测定皇竹草＋黑麦草、玉米＋黑麦草2种周年供青方式的草产量及其营养成分，并对皇竹草的青贮技术及越冬留种技术进行了探讨。[结果]皇竹草＋黑麦草的周年供青方式能更好满足6～11月的青饲料和早春部分黑麦草饲料的供应，结合青贮试验，其营养品质和供青时期都优于传统的玉米＋黑麦草的周年供青方式。因此，对于坡耕地养殖户而言，种植产量高、易管理、品种好、多年生的皇竹草，套种黑麦草可能是更好的选择。皇竹草采用塑料袋青贮法，技术简单且易于操作适合普通农户少量青贮牧草。皇竹草地膜覆盖法留种较其他留种方法更经济可行，皇竹草的种蔸覆盖黄土越冬与其他方法相比更简单易行。[结论]该研究结果可为湖南坡耕地牧草周年高效供青生产模式的建立提供依据。
语种：
中文
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洞庭湖区安乡县渍水冬闲田亚麻生产示范成效分析及发展前景
作者：
邢虎成;陈建芳;熊淑媛;张英;张兴;...
期刊：
现代农业科技 ,2016年(21):47-48 ISSN：1007-5739
作者机构：
湖南农业大学苎麻研究所,湖南长沙,410128;湖南农业大学计财处;安乡县安全乡农技站;湖南农业大学苎麻研究所，湖南长沙 410128;安乡县农业局粮油站
关键词：
渍水冬闲田;亚麻生产;示范成效;洞庭湖区;湖南安乡
摘要：
阐述了洞庭湖区安乡县渍水冬闲田亚麻生产示范的情况,对其成效进行了分析,结果表明:低洼渍水田实施一季稻套种亚麻适应性好,可以免耕不施肥,亚麻种植纯收益为14 55019 950元/hm2。亚麻产值效益比油菜或榨菜要增加收入4 9507 950元/hm2,是一种省工节本、高产高效且能有效抗灾避灾的种植模式。展望了其发展前景,总结了其特点及应注意的问题,以供参考。
语种：
中文
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烤烟生长及品质对主要气候因子的响应
作者：
周锂;揭雨成;肖和友
期刊：
安徽农业科学 ,2015年(13):35-37+97 ISSN：0517-6611
作者机构：
湖南农业大学农学院;[肖和友] 邵阳市烟草公司;[揭雨成; 周锂] 湖南农业大学
关键词：
气候因子;烤烟;生理代谢;形态建成;品质
摘要：
综述了光照、温度及水分这3个气候因子对烤烟生理代谢、形态建成及品质的影响,旨在为烟草行业实际生产和未来研究提供参考。
语种：
中文
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Expression Profiling of Cadmium Response Genes in Ramie (Boehmeria nivea L.) Root
作者：
She, Wei;Zhu, Shoujing;Jie, Yucheng* ;Xing, Hucheng;Cui, Guoxian
期刊：
Bulletin of Environmental Contamination and Toxicology ,2015年94(4):453-459 ISSN：0007-4861
通讯作者：
Jie, Yucheng
作者机构：
[Xing, Hucheng; Cui, Guoxian; She, Wei; Zhu, Shoujing; Jie, Yucheng] Hunan Agr Univ, Ramie Res Inst, Changsha 410128, Hunan, Peoples R China.;[Xing, Hucheng; Cui, Guoxian; Jie, Yucheng] Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Jie, Yucheng] H;Hunan Agr Univ, Ramie Res Inst, Changsha 410128, Hunan, Peoples R China.
关键词：
Cadmium;Gene expression;Phytoremediation;Glutathione;Ramie root
摘要：
Ramie (Boehmeria nivea), a perennial herb belongs to Urticaceae family, is a rapid growth and high biomass crop with highly tolerant and accumulative to heavy metals. However, the gene expression and regulation caused by cadmium (Cd) in ramie has not been well studied. In the present study, a gene expression database of ramie root in the absence (control) or presence of 100 μM Cd was established. Solexa high-throughput sequencing technology showed that 3,654,395 and 3,572,333 tags have been obtained from control and Cd treatment respectively. In total, 3887 genes were detected with significant differential expression levels, in which 2883 genes were up-regulated and 1004 genes were down-regulated. Gene ontology and pathway-based analyses were performed to determine and further to understand the biological functions of those differentially expressed genes. Fifteen genes were selected and their expression levels were confirmed by quantitative RT-PCR, and twelve of them showed consistent expression patterns with the digital gene expression data. Results on these expression profiling of genes lay the basis for biotechnological modification of new transgenic plants with improved phytoremediation capacity. © 2015 Springer Science+Business Media New York.
语种：
英文
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Responses of gas exchange, chlorophyll synthesis and ROS-scavenging systems to salinity stress in two ramie (Boehmeria nivea L.) cultivars
作者：
Huang, C. -J.;Wei, G.;Jie, Y. -C.* ;Xu, J. -J.;Zhao, S. -Y.;...
期刊：
PHOTOSYNTHETICA ,2015年53(3):455-463 ISSN：0300-3604
通讯作者：
Jie, Y. -C.
作者机构：
[Jie, Y. -C.; Huang, C. -J.] Hunan Agr Univ, Inst Ramie, Changsha, Hunan, Peoples R China.;[Wei, G.; Xu, J. -J.; Zhao, S. -Y.; Huang, C. -J.] Dazhou Inst Agr Sci, Dazhou, Sichuan, Peoples R China.;[Wang, L. -C.; Anjum, S. A.] Southwest Univ, Coll Agron & Biotechnol, Chongqing, Peoples R China.
通讯机构：
[Jie, Y. -C.] H;Hunan Agr Univ, Inst Ramie, Changsha, Hunan, Peoples R China.
关键词：
abiotic stress;photosynthesis;pigments;lipid peroxidation;antioxidant enzymes
摘要：
Ramie (Boehmeria nivea L.) is an important crop that serves as fine fiber material, high protein feedstuff, and valuable herbal medicine in China. However, increasing salinity in soil limits the productivity. We investigated in a greenhouse experiment responses to salinity in two ramie cultivars, Chuanzhu-12 (salt-tolerant cultivar, ST) and Xiangzhu-2 (salt-sensitive cultivar, SS), to elucidate the salt tolerance mechanism of this species. Salinity stress substantially reduced both chlorophyll and carotenoid contents. In addition, net photosynthesis, transpiration rate, stomatal conductance, intercellular CO2 concentration, and the ratio of intercellular CO2 to ambient CO2 were affected, less in ST. Nevertheless, salinity stress markedly improved water use efficiency and intrinsic water use efficiency in both species. Moreover, relative water contents, soluble proteins, and catalase activity were substantially impaired, while proline accumulation and superoxide dismutase activity were enhanced substantially, more in ST. Furthermore, noteworthy increase in peroxidase activity and decrease in malondialdehyde content was recorded in ST, whereas, in SS, these attributes changed conversely. Overall, the cultivar ST exhibited salt tolerance due to its higher photosynthetic capacity, chlorophyll content, antioxidative enzyme activity, and nonenzymatic antioxidants, as well as reduced lipid peroxidation and maintenance of the tissue water content. This revealed the salt tolerance mechanism of ramie plants for adaptation to salt affected soil. © 2015, The Institute of Experimental Botany.
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英文
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