作者： 陈梦悦;吴雨恒;廖承清;马方舟;王星

期刊： 生物多样性,2020年28(8):950-957 ISSN：1005-0094

通讯作者： Ma, F.;Wang, X.

作者机构： 湖南农业大学植物保护学院, 长沙, 410128;植物病虫害生物学与防控湖南省重点实验室, 植物病虫害生物学与防控湖南省重点实验室, 长沙, 410128;生态环境部南京环境科学研究所, 国家环境保护生物安全重点实验室, 南京, 210042;[陈梦悦; 吴雨恒; 廖承清; 王星] College of Plant Protection, Hunan Agricultural University, Changsha, 410128, China, Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Changsha, 410128, China;[马方舟] Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, National Key Laboratory of Biosafety, Ministry of Ecology and Environment, Nanjing, 210042, China

通讯机构： [Ma, F.] N;[Wang, X.] C;[Wang, X.] H;Nanjing Institute of Environmental Sciences, China;College of Plant Protection, China

关键词： 物种多样性;月动态;蝴蝶;八大公山;生境;海拔

摘要： 为了解八大公山国家级自然保护区内的蝴蝶群落组成及海拔分布,作者于2016–2018年间对该区域不同生境的蝴蝶群落进行了系统调查和多样性分析,并持续观测其群落的月动态变化。本研究共记录蝴蝶6,164只,隶属于5科107属191种。低海拔(250–450 m)生境记录到的蝴蝶以凤蝶亚科为主,高海拔(1,200–1,400 m)生境以粉蝶亚科为主,而中海拔(700–900 m)生境无明显的蝴蝶优势类群。相似性分析结果表明,各生境存在很高比例的共有种(59%以上),这些共有种在不同生境的个体数分布存在很大的差异。季节动态分析结果表明,八大公山蝴蝶群落的物种数、个体数及多样性指数在4–6月逐渐上升, 7月达到峰值, 8–9月逐渐下降。总体来说,八大公山的蝴蝶群落组成沿海拔梯度呈现垂直分布差异,高海拔与低海拔存在明显的异质性,中海拔则呈现出一种过渡状态;且八大公山不同海拔的蝴蝶群落表现出较为一致的季节动态,都具有很高比例的物种更替;相对于低海拔,高海拔生境蝴蝶群落的活跃期更短,物种的时间周转率更高。

语种： 中文

作者： 陈功;王星;潘浪

期刊： 科教导刊-电子版(中旬),2020年(8):178,183 ISSN：2097-1621

作者机构： 湖南农业大学植物保护学院 湖南·长沙 410128;[陈功; 潘浪; 王星] 湖南农业大学

关键词： 普通昆虫学;一流本科课程

摘要： 《教育部关于一流本科课程建设的实施意见》是未来五年高校本科教育的指导性文件.本文以农林高校重要专业基础课《普通昆虫学》为例,阐述了湖南农业大学一流本科课程建设的基本思路,以及取得的成果和经验教训.

语种： 中文

作者： Chen, Gong;Liu, Hang;Mo, Bo-Cheng;Hu, Jue;Liu, Shuang-Qing;...

期刊： Frontiers in Physiology,2020年11:508913 ISSN：1664-042X

通讯作者： Wang, Xing

作者机构： [Chen, Gong; Liu, Hang; Liu, Shuang-Qing; Xue, Jing; Wang, Xing; Hu, Jue; Mo, Bo-Cheng] Hunan Agr Univ, Coll Plant Protect, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha, Peoples R China.;[Chen, Gong; Liu, Hang; Liu, Shuang-Qing; Xue, Jing; Wang, Xing; Hu, Jue] Hunan Agr Univ, Coll Plant Protect, Changsha, Peoples R China.;[Chen, Gong; Bustos-Segura, Carlos] Univ Neuchatel, Fac Sci, Inst Biol, Neuchatel, Switzerland.

通讯机构： [Wang, Xing] H;Hunan Agr Univ, Coll Plant Protect, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha, Peoples R China.;Hunan Agr Univ, Coll Plant Protect, Changsha, Peoples R China.

关键词： Helicoverpa armigera;Heliothis virescens ascovirus 3i;insect virus;mortality;pathogenic ability

摘要： Although the cotton bollworm Helicoverpa armigera has traditionally been controlled by application of chemical pesticides, chemical control selects for resistance, pollutes the environment, and endangers human health. New methods for controlling H. armigera are therefore needed. Heliothis virescens ascovirus 3i (HvAV-3i) is a recently identified virus of the lepidopteran larvae. We tested the effects of HvAV-3i on H. armigera larvae following oral ingestion of HvAV-3i-containing hemolymph (about 1.0 × 1010 virus genome copies per larvae) and following injection of HvAV-3i-containing hemolymph by insertion of a needle. Following oral ingestion, first-instar to fifth-instar larvae grew and developed normally. Following needle injection, in contrast, the corrected mortality of third and fourth instars was 88.9 ± 2.1 and 93.7 ± 3.4%, respectively. Food intake was significantly lower for larvae injected with virus-containing hemolymph than with virus-free hemolymph. Larvae injected with virus-containing hemolymph had extended survival times and could not complete the pre-pupal stage. These results indicate that inoculation of HvAV-3i via needle injection, but not via oral ingestion, significantly reduced the growth and development of H. armigera larvae. © Copyright © 2020 Chen, Liu, Mo, Hu, Liu, Bustos-Segura, Xue and Wang.

语种： 英文

作者： 杜星星;王雅倩;王明鉴;张文武;杨华;...

期刊： 华中昆虫研究,2020年(00)

作者机构： 湖南农业大学植物保护学院;湖南乌云界国家级自然保护区管理局

关键词： 大造桥虫;外部形态;行为习性;辣椒;受害症状

摘要： 大造桥虫Ascotis selenaria Schiffermuller et Denis属鳞翅目Lepidoptera尺蛾科Geometridae,又名尺蠖、步曲、棉大造桥虫等,是一种间歇性、爆发性、局部为害严重的世界性害虫,广泛分布于亚洲、欧洲和非洲。在国内主要分布于东北、华北、华东、华中和西南等地区。大造桥虫食性复杂,主要寄主为辣椒、茄子以及十字花科、豆科等经济作物,也可危害多种果树。主要以幼虫取食植物叶片,严重时取食整片叶,只留下枝干,极大影响了植物的光合作用,降低了作物及果树的经济价值和观赏价值。为此,本文以采于贵州省安顺市西秀区七眼桥镇山岚村试验田大造桥虫幼虫为供试虫源,将幼虫置于培养皿（直径10 cm）饲养,每天更换新鲜辣椒叶片,记录（拍照）各虫态的发育、形态（如体型、颜色、体表特征等）、行为习性与辣椒叶片受害症状,可为当地辣椒大造桥虫危害与防治提供参考依据。（1）形态特征:该虫幼虫（图1-A～B）躯体柔软,呈椭圆形,胸足3对,腹足2对。低龄幼虫黄白色,有白色横纹,第二腹节背面有两个不规则黑斑。高龄幼虫黑棕色,覆有体毛,头尾部居多;体表有多条棕色纵纹和黑斑;两对腹足距离缩短,气门线较低龄更明显。其蛹（图1-C）纺锤形,体长为14 mm左右。初为浅褐色,逐渐变为深褐色,有光泽。头顶有疣状突起,尾端尖,臀棘2根;成虫（图1-D）体长15～20 mm,翅展38～45 mm,体为浅灰褐色。翅面横线和斑纹均为暗褐色,前翅亚基线和外横线锯齿状,其间为灰黄色。雌触角丝状,雄羽状,淡黄色。（2）行为习性:初孵幼虫受到惊吓时,会吐丝下垂,随风飘荡到其它植物上继续取食。幼虫曲弓爬行,爬行时弯腰如拱桥;静止时靠2对腹足固定身体,斜向伸直如一个干枯的小树枝（图1-E）。1～3龄幼虫常昼夜取食,4～5龄夜晚活动取食。高龄幼虫有拟态和假死性,可用来躲避敌害。幼虫老熟后白天吐丝下垂或直接掉在地面,入松土化蛹。化蛹后不食不动,不存在排便行为。触碰尾部会转圈晃动,多个蛹距离近时会出现连锁反应一起晃动尾部。成虫多在傍晚羽化,羽化后1～3天开始交尾,交尾后1～2天开始产卵,产卵多在夜间,产于叶背、叶柄、树缝等处。（3）受害症状:大造桥虫幼虫咀嚼式口器,取食叶片,沿叶脉、叶缘将叶片咬成孔洞、缺刻,甚至将叶片吃光（仅剩主叶脉,如图1-F）。初孵幼虫会先集中在顶部嫩叶处,将顶部嫩叶咬成圆形网状或小孔洞。3龄幼虫开始分散取食,将叶片啃食成缺刻状。4～5龄幼虫取食量剧增,取食整片叶片,仅剩主叶脉,6龄幼虫取食量最大。被为害的辣椒叶片呈弧形,里圈为小锯齿状形状。幼虫先沿叶片的最周边开始进行取食,咬成圆弧状,后沿叶片向下进行取食,在叶片的周围形成一个个弧状,最后弧形直至相通,只剩叶脉。危害严重时穿插在叶肉间的大叶脉也被取食。近年来随着当地辣椒栽培面积的不断扩大,辣椒的虫害也日渐严重。大造桥虫主要取食辣椒叶片,特点是食量大,爆发性强,是辣椒虫害中比较严重的害虫之一。不仔细观察或防治不及时,会在短时间内将植株叶片吃光,后借助抽丝下垂转移到其他植株为害,给辣椒的栽培造成极大困扰。本次实验观察到大造桥虫幼虫期虫体颜色较浅,存在吐丝和排便行为。取食辣椒叶片,严重时整片叶尽食。爬行过程中,虫体依靠后半部分的两对腹足抓附在固定物上,曲弓前行。受到侵扰时,幼虫会出现"假死"行为;侵扰解除后,幼虫恢复活动。进入化蛹时期,不食不动,当触动尾部时,左右晃动。随着进入化蛹的时间越长,外壳颜色会逐渐加深,尾部体节数逐渐明显,可清楚观察到大造桥虫的头部、两翅的位置及尾部的结构。观察造桥虫形态特征、行为特性等,充分了解其危害辣椒的症状,以对症下药、科学防治。针对不同时期的虫态采取不同的方法,如人工防治、灯光诱杀、化学防治等,对其达到高效的防治,提前控制预防害虫大发生,提高辣椒品质及产量,降低农民的经济损失。

语种： 中文

作者： 曹生凯;廖梦琪;张文武;杨华;王星

期刊： 华中昆虫研究,2020年(00)

作者机构： 湖南农业大学植物保护学院;湖南乌云界国家级自然保护区管理局

关键词： 辣椒;烟青虫;防控对策;研究进展

摘要： 烟青虫是一种世界性害虫,主要以幼虫为害作物的各生长期及不同生长部位,严重影响作物的生长发育和农产品的品质。目前对烟青虫的防治仍以化学防治为主,新型的防治技术相对较少且不被广大农户所接受。本文详细总结了辣椒烟青虫各类防治对策的研究进展,展望了未来的发展趋势,以期为今后烟青虫的防治应用提供理论参考。

语种： 中文

作者： Chen, Meng-Yue;Chen, Lu;Wang, Xing*

期刊： Mitochondrial DNA Part B-Resources,2020年5(2):1176-1177 ISSN：2380-2359

通讯作者： Wang, Xing

作者机构： [Chen, Lu; Wang, Xing; Chen, Meng-Yue] Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.;[Chen, Lu; Wang, Xing; Chen, Meng-Yue] Hunan Agr Univ, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Wang, Xing] H;Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha 410128, Hunan, Peoples R China.

关键词： Hydrelia parvulata (Staudinger;mitochondrial genome;evolutionary position

摘要： Abstract In this study, the complete mitochondrial genome of Hydrelia parvulata (Staudinger, 1897) was firstly sequenced by high-throughput sequencing. As a circular DNA molecule, it is 15,407 bp in length (GenBank accession number: MN962739), which consists of 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes, and an AT-rich region. The nucleotide composition is A (39.8%), C (11.6%), G (8.2%), and T (40.4%). Based on the sequences of complete mitogenome from 10 geometrid species as ingroups and 3 bombycid species as outgroups, the phylogenetic trees were constructed using maximum likelihood (ML) method. The results showed that H. parvulata has a close evolutionary relationship with Operophtera brumata in the subfamily Larentiinae, which is a clade strongly supported by the bootstrap value of 100% and a sister-group with the subfamily Ennominae.

语种： 英文

作者： 赵琳超;廖用信;陈壮美;刘航;王星

期刊： 湖南师范大学自然科学学报,2020年43(1):41-47 ISSN：2096-5281

作者机构： 湖南农业大学植物保护学院;[廖用信] 武冈市农业农村局;[赵琳超; 刘航; 陈壮美; 王星] 湖南农业大学

关键词： 草地贪夜蛾;温度;生长发育;发育起点温度;有效积温

摘要： 为明确温度对草地贪夜蛾生长发育的影响,测定了6个温度（18,21,24,27,30和33℃）条件下草地贪夜蛾幼虫和蛹的发育历期、蛹重和存活率等指标,计算出各虫态的发育起点温度、有效积温和各温度条件下的发育速率。结果表明:在18～33℃范围内,草地贪夜蛾幼虫和蛹的发育历期均随着温度的升高而逐渐缩短,发育速率逐渐加快,其生长发育的最适宜温度范围为24～33℃;幼虫和蛹的发育起点温度分别为10.61±3.19℃和13.29±1.34℃,有效积温分别为183.19±9.96 d·℃和134.83±13.71 d·℃。该虫的蛹重随着温度的升高呈先升高后降低的变化趋势,在21℃条件下,其蛹重达到最大值251.91±11.19 mg。

语种： 中文

作者： 陈梦悦;王明鉴;杨华;张文武;王星

期刊： 华中昆虫研究,2019年(00):95-101

作者机构： 植物病虫害生物学与防控湖南省重点实验室/湖南农业大学植物保护学院,长沙410128;湖南乌云界国家级自然保护区管理局,桃源415700

关键词： 桦蛾科;系统学;形态学;分子生物学;系统发育

摘要： 桦蛾科Endromidae隶属于昆虫纲Insects鳞翅目Lepidoptera,全世界性分布,已知16属70种。桦蛾是一类中型蛾子,幼虫多取食植物叶片,有些种类为农林业、园艺园林业的重要害虫。因此,对其开展系统学研究,进行准确的种类鉴定对于农林业生产具有重要的研究价值和经济意义。目前世界范围内已开展的桦蛾科研究均为区域性工作,但仍缺少全面的区系研究和系统的分类修订工作。另外,桦蛾科是鳞翅目昆虫系统树上的关键点之一,对其进行深入的形态、分子研究,重建其系统发育以修订完善鳞翅目高级阶元分类系统,也将对中国鳞翅目系统分类研究提供参考,对农林害虫的防治工作提供借鉴。

语种： 中文

作者： 吴永美;刘洋;魏仲珊;张志飞;王星

期刊： 华中昆虫研究,2019年(00):286-293

作者机构： 湖南农业大学植物保护学院/植物病虫害生物学与防控湖南省重点实验室,长沙410128;湖南德人牧业科技有限公司,常德415921;湖南农业大学农学院,长沙210128;不详

关键词： 苜蓿;害虫;天敌;功能团

摘要： 苜蓿是当今世界分布最广的栽培牧草,苜蓿害虫种类繁多,严重威胁着畜牧养殖业。经过实地调查并结合相关文献对我国苜蓿害虫及其天敌物种多样性和功能团分析,将苜蓿害虫划分为6个功能团,将天敌划分为2个功能团。结果显示:我国苜蓿害虫有301种,隶属于7目50科205属,其中以鳞翅目和鞘翅目种类最多,而功能团中则以食茎叶类害虫种类最丰富;苜蓿害虫自然天敌有68种,隶属于8目26科48属,以鞘翅目为主的捕食性天敌种类最多。本文总结了苜蓿上的害虫及其天敌数据并按功能团进行分析,旨在为苜蓿害虫的综合防治提供一定的参考依据。

语种： 中文

作者： 杨婷;肖伟;李逸豪;邱林;刘俊杰;...

期刊： 华中昆虫研究,2019年(00):294-300

作者机构： 植物病虫害生物学与防控湖南省重点实验室/湖南农业大学植物保护学院,长沙410128;环境保护部南京环境科学研究所/国家环境保护生物安全重点实验室,南京210042;不详

关键词： 蝴蝶多样性;南岳衡山;保护区;湖南

摘要： 为了对湖南省衡山国家级自然保护区的蝶类物种多样性保护状况进行评估。本研究于2018年4—9月,在该保护区选取5条不同生境的样线逐月进行蝶类多样性调查。共记录蝶类1 260只,隶属于5科59属84种。其中蛱蝶科的物种最为丰富（38种,占45.24%）,其后依次是凤蝶科、灰蝶科、粉蝶科、弄蝶科。不同生境的蝴蝶群落存在一定差异,其中位于保护区核心区生境b（常绿阔叶林）的多样性指数最高（3.059）,而位于保护区试验区e（农田）的多样性指数最低（2.559）,说明保护区对于当地蝴蝶多样性的保护发挥了积极的作用。

语种： 中文

作者： Chen, Lu;Liao, Cheng-Qing;Wang, Xing;Tang, Shao-Xian*

期刊： Mitochondrial DNA Part B-Resources,2019年4(2):2769-2770 ISSN：2380-2359

通讯作者： Tang, Shao-Xian

作者机构： [Liao, Cheng-Qing; Chen, Lu; Wang, Xing] Hunan Agr Univ, Coll Plant Protect, Changsha, Hunan, Peoples R China.;[Liao, Cheng-Qing; Chen, Lu; Wang, Xing] Hunan Agr Univ, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha, Hunan, Peoples R China.;[Tang, Shao-Xian] Hunan Agr Univ, Coll Informat Sci & Technol, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Tang, Shao-Xian] H;Hunan Agr Univ, Coll Informat Sci & Technol, Changsha 410128, Hunan, Peoples R China.

关键词： evolutionary position;Gibbovalva kobusi;mitochondrial genome

摘要： In this study, the complete mitochondrial genome (mitogenome) of Gibbovalva kobusi was at first sequenced by high-throughput sequencing. As a circular DNA molecule, the complete mitogenome is 15,717 bp in length (GeneBank accession number: MK956103) and consists of 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes, and an AT-rich region. The nucleotide composition is A (41.0%), C (11.6%), G (7.9%), and T (39.5%). Based on the sequences of complete mitogenome from 11 species as ingroups and 3 superfamily Tineoidea species as outgroups, the phylogenetic trees were constructed. The family Gracilariidae as a monophyletic clade is strongly supported by the bootstrap value of 100%. © 2019, © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

语种： 英文

作者： Xu, Ting;Cao, Lidan;Zeng, Jiarui;Franco, Christopher M. M.;Yang, Yuanzhu;...

期刊： Pesticide Biochemistry and Physiology,2019年160:58-69 ISSN：0048-3575

通讯作者： Liu, Xuanming;Zhu, Yonghua

作者机构： [Zeng, Jiarui; Zhu, Yonghua; Gao, Yan; Wang, Xiang; Liu, Ying; Liu, Xuanming; Zhu, YH; Bu, Zhigang; Cao, Lidan; Xu, Ting] Hunan Univ, Coll Biol, Hunan Prov Key Lab Plant Funct Genom & Dev Regula, Changsha 410082, Hunan, Peoples R China.;[Franco, Christopher M. M.] Flinders Univ S Australia, Sch Med, Dept Med Biotechnol, Adelaide, SA 5042, Australia.;[Hu, Xiaochun; Yang, Yuanzhu] Yahua Seeds Sci Acad Hunan, Changsha 410119, Hunan, Peoples R China.;[Shi, Liming] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100081, Peoples R China.;[Zhou, Guoying] Cent South Univ Forestry & Technol, Hunan Prov Key Lab Control Forest Dis & Pests, Changsha 410008, Hunan, Peoples R China.

通讯机构： [Liu, XM; Zhu, YH] H;Hunan Univ, Coll Biol, Hunan Prov Key Lab Plant Funct Genom & Dev Regula, Changsha 410082, Hunan, Peoples R China.

关键词： antifungal agent;biological pest control;chemistry;drug effect;endophyte;Magnaporthe;microbiology;Oryza;physiology;Streptomyces;Antifungal Agents;Endophytes;Magnaporthe;Oryza;Pest Control, Biological;Streptomyces

摘要： Microbial antagonists and their bioactive metabolites provide one of the best alternatives to chemical pesticides to control crop disease for sustainable agriculture and global food security. The rice endophyte Streptomyces hygroscopicus OsiSh-2, with remarkable antagonistic activity towards the rice blast fungus Magnaporthe oryzae, was reported in our previous study. The present study deciphered the possible direct interaction mode of OsiSh-2 against M. oryzae. An in vitro antibiotic assay for OsiSh-2 culture filtrate revealed strong suppression of mycelial growth, conidial germination and appressorial formation of M. oryzae. Meanwhile, severe morphological and internal abnormalities in M. oryzae hyphae were observed under a scanning electron microscope and transmission electron microscope. Foliar treatment of rice seedlings by OsiSh-2 culture filtrate in the greenhouse and in the field showed 23.5% and 28.3% disease reduction, respectively. Correspondingly, OsiSh-2 culture filtrate could induce disorganized chitin deposition in the cell wall and lowered ergosterol content in the cell membrane of M. oryzae. Additionally, cell wall integrity pathway activation, large cell electrolytes release, reactive oxygen species accumulation and tricarboxylic acid cycle-related enzyme activity changes were found in M. oryzae. All these results suggested that the direct antagonistic activity of OsiSh-2 against M. oryzae may be attributed to damaging the integrity of the cell wall and membrane and disrupting mitochondrial function in the pathogen. © 2019 Elsevier Inc.

语种： 英文

作者： Wang, Xing*;Chen, Zhuang-Mei;Gu, Xing-Shi;Wang, Min;Huang, Guo-Hua;...

期刊： SYSTEMATIC ENTOMOLOGY,2019年44(3):490-498 ISSN：0307-6970

通讯作者： Wang, Xing;Zwick, Andreas

作者机构： [Gu, Xing-Shi; Huang, Guo-Hua; Wang, Xing; Chen, Zhuang-Mei] Hunan Agr Univ, Coll Plant Protect, Nongda Rd 1, Changsha 410128, Hunan, Peoples R China.;[Gu, Xing-Shi; Huang, Guo-Hua; Wang, Xing; Chen, Zhuang-Mei] Hunan Agr Univ, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha, Hunan, Peoples R China.;[Wang, Min] South China Agr Univ, Coll Agr, Guangzhou, Guangdong, Peoples R China.;[Zwick, Andreas] CSIRO, Australian Natl Insect Collect, Acton, Australia.;[Zwick, Andreas] CSIRO, Australian Natl Insect Collect, Canberra, ACT 2601, Australia.

通讯机构： [Wang, Xing] H;[Zwick, Andreas] C;Hunan Agr Univ, Coll Plant Protect, Nongda Rd 1, Changsha 410128, Hunan, Peoples R China.;CSIRO, Australian Natl Insect Collect, Canberra, ACT 2601, Australia.

摘要： The family Bombycidae (sensu Minet, 1994) is a diverse group of species belonging to the superfamily Bombycoidea. It is an economically important group of moth species, containing well-known silk-producing insects, as well as many pests of agriculture and forestry. The morphology-based hypothesis of Minet (1994) on the composition of Bombycidae is in conflict with subsequent phylogenetic hypotheses for the superfamily based on nuclear genes. In this paper, the complete mitochondrial genomes of nine species of Bombycidae are presented for the first time. Based on these genomes, four dataset partitions and three gblocks parameter settings, phylogenetic relationships among Bombycidae were reconstructed using maximum likelihood and Bayesian inference methods. Bombycidae was confirmed as a polyphyletic group, with the traditional subfamilies Prismostictinae and Oberthueriinae forming a single well-supported clade that is distant to Bombycinae. The phylogenetic relationships within Bombycoidea were supported as ((((Bombycinae, Sphingidae), Saturniidae), (Prismostictinae, Oberthueriinae)), Eupterotidae).

语种： 英文

作者： Qiu, L.;Sun, Y.;Jiang, Z.;Yang, P.;Liu, H.;...

期刊： Insect Molecular Biology,2019年28(4):520-527 ISSN：0962-1075

通讯作者： Ma, W.

作者机构： [Lin, Y.; Qiu, L.; Yang, P.; Liu, H.; Sun, Y.; Zhang, W.; Zhou, H.; Ma, W.] Natl Key Lab Crop Genet Improvement, Wuhan, Hubei, Peoples R China.;[Lin, Y.; Qiu, L.; Yang, P.; Liu, H.; Sun, Y.; Zhang, W.; Zhou, H.; Ma, W.] Natl Ctr Plant Gene Res, Wuhan, Hubei, Peoples R China.;[Qiu, L.] Hunan Agr Univ, Coll Plant Protect, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha, Hunan, Peoples R China.;[Jiang, Z.; Yang, P.; Liu, H.; Wang, X.; Sun, Y.; Zhou, H.; Ma, W.] Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan, Hubei, Peoples R China.;[Ma, W.] Huazhong Agr Univ, Coll Plant Sci & Technol, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Hubei, Peoples R China.

通讯机构： [Ma, W.] H;Huazhong Agr Univ, Coll Plant Sci & Technol, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Hubei, Peoples R China.;Huazhong Agr Univ, Coll Plant Sci & Technol, Natl Ctr Plant Gene Res, Wuhan 430070, Hubei, Peoples R China.

关键词： Bacillus thuringiensis;Cry toxin;V-ATPase subunit A;Bt receptor;Chilo suppressalis;RNA interference

摘要： Insecticidal crystal (Cry) proteins produced by the bacterium Bacillus thuringiensis (Bt) are toxic to a diverse range of insects. Transgenic rice expressing Cry1A, Cry2A and Cry1C toxins have been developed that are lethal to Chilo suppressalis, a devastating insect pest of rice in China. Identifying the mechanisms underlying the interactions of Cry toxins with susceptible hosts will improve both our understanding of Cry protein toxicology and long-term efficacy of Bt crops. In this study, we tested the hypothesis that V-ATPase subunit A contributes to the action of Cry1Ab/1Ac, Cry2Aa and Cry1Ca toxins in C. suppressalis. The full-length V-ATPase subunit A transcript was initially cloned from the C. suppressalis larval midgut and then used to generate double-stranded RNA (dsRNA)-producing bacteria. Toxicity assays using transgenic rice lines TT51 (Cry1Ab and Cry1Ac fusion genes), T2A-1 (Cry2Aa), and T1C-19 (Cry1Ca) in conjunction with V-ATPase subunit A dsRNA-treated C. suppressalis larvae revealed significantly reduced larval susceptibility to T2A-1 and T1C-19 transgenic rice, but not to TT51 rice. These results suggest that the V-ATPase subunit A plays a crucial role in mediating Cry2Aa and Cry1Ca toxicity in C. suppressalis. These findings will have significant implications on the development of future resistance management tools.

语种： 英文

作者： 陈壮美;赵琳超;刘航;廖用信;王星

期刊： 植物保护,2019年45(5):71-74,90 ISSN：0529-1542

作者机构： 湖南农业大学植物保护学院, 植物病虫害生物学与防控湖南省重点实验室, 长沙, 410128;湖南省武冈市农业农村局, 武冈, 422400;[赵琳超; 刘航; 陈壮美; 王星] 湖南农业大学;[廖用信] 武冈市农业农村局

关键词： 斯氏侧沟茧蜂;草地贪夜蛾;寄生行为;寄生效果;生物防治

摘要： 草地贪夜蛾入侵我国后快速扩散,发现和保护利用本土天敌是有效抑制其暴发为害的重要途径。本研究以长沙地区农田常见的斯氏侧沟茧蜂为研究对象,系统观察了其对不同龄期草地贪夜蛾幼虫的寄生行为,测定了被寄生草地贪夜蛾幼虫体重和取食量的变化。结果表明,斯氏侧沟茧蜂能寄生2~4龄草地贪夜蛾幼虫,可显著抑制其体重增量和取食量,有效阻滞其进入暴食期,并最终致其死亡。说明斯氏侧沟茧蜂可有效寄生草地贪夜蛾幼虫,并抑制其生长和取食,对其具有一定生物防控能力,是值得保护和利用的本土寄生蜂。

语种： 中文

作者： 廖用信;王星

期刊： 华中昆虫研究,2019年(00):319-322

作者机构： 湖南省武冈市农业农村局,武冈422400;植物病虫害生物学与防控湖南省重点实验室/湖南农业大学植物保护学院,长沙410128

关键词： 草地贪夜蛾;玉米;囊泡病毒;侧沟茧蜂;为害;防治

摘要： 草地贪夜蛾作为世界性重大迁飞性害虫,自2019年1月侵入我国后,短短几个月内在我国蔓延。尽管我国本土已发现对草地贪夜蛾有较好控制作用的自然天敌,如昆虫囊泡病毒、斯氏侧沟茧蜂等,但该虫将可能造成的影响不容忽视,借此我们于2019年3月开始对武冈市的玉米地进行持续的监控,在5月9日首次发现草地贪夜蛾入侵武冈市,随之开展系统调查,基本摸清草地贪夜蛾在我市的发生情况及为害程度、为害特点,并做出群防群治、早发现早防治的策略方案。

语种： 中文

作者： 王凤英;肖乾鹏;王星

期刊： 广东农业科学,2019年46(10):82-86 ISSN：1004-874X

作者机构： 广州安芮洁环保科技有限公司,广东 广州 510450;湖南农业大学植物保护学院,湖南 长沙 410128;广州安芮洁环保科技有限公司,广东 广州,510450;湖南农业大学植物保护学院,湖南 长沙,410128;[王凤英; 肖乾鹏] 广州安芮洁环保科技有限公司

关键词： 黑水虻;虫卵;冷藏保存;孵化率;幼虫生长发育

摘要： 【目的】持续低温或阴雨天气对黑水虻成虫产卵量造成较大影响,在产卵高峰期冷藏多余虫卵用于卵量低谷期,将有利于规模化标准化生产黑水虻。【方法】研究了不同发育阶段黑水虻虫卵经12 ℃冷藏不同天数后的孵化情况,以及原肠期虫卵经12 ℃分别冷藏5、10、15 d后在32 ℃下孵化生长发育情况。【结果】初产虫卵不能直接冷藏,12 ℃冷藏1 d后转32℃孵化,死亡率达100%。胚盘胚带形成期、原肠期、体壁背合期和破壳期的虫卵在12 ℃下冷藏10 d转置32 ℃孵化,平均孵化率分别为94%、94%、27%、0%,体壁背合期和破壳期虫卵不适宜冷藏。胚盘胚带形成期虫卵冷藏13 d后孵化率降至80%以下;原肠期虫卵冷藏15 d后孵化率降至80%以下。冷藏5、10 d原肠期虫卵孵化的幼虫生长发育与对照无显著差异;冷藏15 d原肠期虫卵存活率低于对照,百头虫重无显著差异。【结论】在12 ℃冷藏的原肠期虫卵能保持较高的存活率,幼虫生长发育正常,该结果为黑水虻的工厂化生产提供理论依据与实践指导。

语种： 中文

作者： Chen, Zi-Shu;Cheng, Xiao-Wen;Wang, Xing;Hou, Dian-Hai*;Huang, Guo-Hua*

期刊： JOURNAL OF GENERAL VIROLOGY,2019年100(2):301-307 ISSN：0022-1317

通讯作者： Huang, Guo-Hua;Hou, Dian-Hai

作者机构： [Chen, Zi-Shu; Huang, Guo-Hua; Wang, Xing] Hunan Agr Univ, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha 410128, Hunan, Peoples R China.;[Chen, Zi-Shu; Huang, Guo-Hua; Wang, Xing] Hunan Agr Univ, Coll Plant Protect, Inst Virol, Changsha 410128, Hunan, Peoples R China.;[Cheng, Xiao-Wen] Miami Univ, Dept Microbiol, 212 Pearson Hall, Oxford, OH 45056 USA.;[Hou, Dian-Hai] Weifang Med Univ, Sch Biosci & Technol, Weifang 261053, Shandong, Peoples R China.

通讯机构： [Huang, Guo-Hua] H;[Hou, Dian-Hai] W;Hunan Agr Univ, Coll Plant Protect, Inst Virol, Changsha 410128, Hunan, Peoples R China.;Weifang Med Univ, Sch Biosci & Technol, Weifang 261053, Shandong, Peoples R China.

关键词： DNA;membrane protein;proteome;RNA;viral protein;proteome;viral protein;Article;Ascovirus;carbohydrate metabolism;cytolysis;DNA metabolism;DNA transcription;DNA virus infection;Heliothis virescens;homeostasis;lipid metabolism;nonhuman;priority journal;protein synthesis;proteomics;RNA metabolism;RNA transcription;virion;virus replication;virus strain;Ascoviridae;biology;chemistry;proteomics;virion;Ascoviridae;Computational Biology;Proteome;Proteomics;Viral Proteins;Virion

摘要： Ascoviruses are enveloped, circular, double-stranded DNA viruses that can effectively control the appetite of lepidopteran larvae, thereby reducing the consequent damage and economic losses to crops. In this study, the virion of a sequenced Heliothis virescens ascovirus 3i (HvAV-3i) strain was used to perform proteomic analysis using both in-gel and in-solution digestion. A total of 81 viral proteins, of which 67 were associated with the virions, were identified in the proteome of HvAV-3i virions. Among these proteins, 23 with annotated functions were associated with DNA/RNA metabolism/transcription, virion assembly, sugar and lipid metabolism, signalling, cellular homoeostasis and cell lysis. Twenty-one viral membrane proteins were also identified. Some of the minor ‘virion’ proteins identified may be non-virion contaminants of viral proteins synthesized during replication, identified by more recent and highly sensitive methods. The extensive identification of the ascoviral proteome will establish a foundation for further investigation of ascoviral replication and infection. © 2019 The Authors.

语种： 英文

作者： Liao, Ming-Wei;Gu, Xing-Shi;Xue, Jin;Wang, Xing*

期刊： Mitochondrial DNA Part B-Resources,2018年3(1):17-18 ISSN：2380-2359

通讯作者： Wang, Xing

作者机构： [Gu, Xing-Shi; Xue, Jin; Liao, Ming-Wei; Wang, Xing] Hunan Agr Univ, Coll Plant Protect, Changsha, Hunan, Peoples R China.;[Gu, Xing-Shi; Xue, Jin; Liao, Ming-Wei; Wang, Xing] Hunan Agr Univ, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha, Hunan, Peoples R China.;[Wang, Xing] Hunan Agr Univ, Dept Plant Protect, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Wang, Xing] H;Hunan Agr Univ, Dept Plant Protect, Changsha 410128, Hunan, Peoples R China.

关键词： Mitochondrial genome;Oberthueria lunwan;Oberthuerinae;evolutionary position

摘要： In this study, the complete mitochondrial genome (mitogenome) of Oberthueria lunwan Zolotuhin & Wang, 2013 (Lepidoptera: Oberthuerinae) is reported for the first time. The entire mitogenome is a circular DNA molecule of 15,673 bp in length (GenBank accession number: MF100143), consisting of 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs) genes, two ribosomal RNAs (rRNAs) genes, and a control region (A + T-rich region). The phylogenetic trees are based on 13 PCGs amino acid sequences of 31 related lepidopteran species in which mitogenome sequences were constructed. The Oberthuerinae consisting of O. lunwan+Andraca theae was strongly supported as a monophyletic clade by the posterior probability of 1.00 and the bootstrap value of 100%, but the relationships among Oberthuerinae, Bombycidae, Satruniidae, and Sphingidae are need to be further confirmed. © 2018, © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

语种： 英文

作者： 吴雨恒;谷志容;肖伟;李逸豪;刘俊杰;...

期刊： 华中昆虫研究,2018年(00):276-281

作者机构： 湖南农业大学植物保护学院/植物病虫害生物学与防控湖南省重点实验室,长沙410128;湖南八大公山国家级自然保护区管理处,桑植427100

关键词： 蝶类;新记录;八大公山国家级自然保护区

摘要： 2017年4—9月,对湖南八大公山国家级自然保护区的蝶类种群资源共进行了6次调查。记录蝶类2 598只,隶属于5科92属149种。其中12种为保护区新记录种,分别为柳紫闪蛱蝶Apatura ilia (Denis&Schiffermuller,1775)、离斑带蛱蝶Athyma ranga Moore, 1857、大卫绢蛱蝶Calinaga davidis Oberth(u|¨)r,1879、白带鳌蛱蝶Charaxes bernardus Fabricius,1793、明窗蛱蝶Dilipa fenestra(Leech, 1891)、孔子翠蛱蝶Euthalia confucius(Westwood, 1850)、傲白蛱蝶Helcyra superba Leech, 1890、矛环蛱蝶Neptis armandia Oberth(u|¨)r,1876、司环蛱环Neptis speyeri Staudinger,1887、霭菲蛱蝶Phaedyma aspasia Leech,1890、白裳猫蛱蝶Timelaea albescens (Oberth(u|¨)r,1886)、姜弄蝶Udaspes folus Cramer, 1775。

语种： 中文