期刊：
Canadian Journal of Infectious Diseases and Medical Microbiology,2023年2023:9933783 ISSN：1712-9532
通讯作者：
Liao, XL;Liu, Y
作者机构：
[Gu, Zepei; Liao, XL; Du, Xiaohua; Su, Pin; Zhang, Deyong; Liao, Xiaolan] Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Peoples R China.;[Zhang, Weixing; Gu, Zepei; Du, Xiaohua; Su, Pin; Zhang, Deyong; Chen, Lijie; Liu, Zhuoxin; Liu, Yong; Peng, Qianze] Hunan Acad Agr Sci, Hunan Plant Protect Inst, Changsha 410125, Peoples R China.;[Su, Pin; Zhang, Deyong; Chen, Lijie; Liu, Zhuoxin; Liu, Yong] Hunan Univ, Longping Branch, Grad Sch, Changsha 410125, Peoples R China.
通讯机构：
[Liao, XL ; Liu, Y ] H;Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Peoples R China.;Hunan Acad Agr Sci, Hunan Plant Protect Inst, Changsha 410125, Peoples R China.;Hunan Univ, Longping Branch, Grad Sch, Changsha 410125, Peoples R China.
关键词：
Introduction;Materials and Methods;Results;Discussion;Conclusion;Abstract;Data Availability;Additional Points;Ethical Approval;Consent;Disclosure;Conflicts of Interests;Authors’ Contributions;Funding Statement;Acknowledgements;Acknowledgments;Supplementary Materials;Reference;Dataset Description;Dataset Files;Abstract;Introduction;Introduction and Materials;Introduction and Methods;Materials;Materials and Methods;Methods;Results;Discussion;Results and Discussion;Discussion and Conclusion;Results and Conclusion;Conclusion;Conclusions;Data Availability;Additional Points;Ethical Approval;Consent;Disclosure;Conflicts of Interest;Authors’ Contributions;Funding Statement;Acknowledgements;Supplementary Materials;References;Appendix;Abbreviations;Preliminaries;Introduction and Preliminaries;Notation;Proof of Theorem;Proofs;Analysis of Results;Examples;Numerical Example;Applications;Numerical Simulation;Model;Model Formulation;Systematic Palaeontology;Nomenclatural Acts;Taxonomic Implications;Experimental;Synthesis;Overview;Characterization;Background;Experimental;Theories;Calculations;Model Verification;Model Implementation;Geographic location;Study Area;Geological setting;Data Collection;Field Testing;Data and Sampling;Dataset;Literature Review;Related Works;Related Work;System Model;Methods and Data;Experimental Results;Results and Analysis;Evaluation;Implementation;Case Presentation;Case Report;Search Terms;Case Description;Case Series;Background;Limitations;Additional Points;Case;Case 1;Case 2 etc.;Concern Details;Retraction Details;Copyright;Related Articles
摘要：
Beauveria bassiana is a well-known insecticidal biocontrol agent. Despite its broad field applications, its survival, colonization, and stability under field conditions remained unclear, mainly due to the lack of a quick and reliable detection method. In this study, we developed a quantitative real-time PCR technology to monitor the stability and population dynamics of B. bassiana in different substrates (water, soil, and on the cotton leaves surface), different spores of B. bassiana applied on Chinese cabbage leaves surface, and the lethality of Pieris rapae spraying with different spores of B. bassiana. Our results showed a decreased concentration of B. bassiana DNA in all three substrates from the 1(st) day till 9(th) day of post inoculation (dpi) period, possibly due to the death of B. bassiana. After this decrease, a quick and significant rebound of B. bassiana DNA concentration was observed, starting from the 11(th) dpi in all three substrates. The B. bassiana DNA concentration reached the plateau at about 13(th) dpi in water and 17(th) dpi in the soil. On cotton leaves surface, the B. bassiana DNA concentration reached the highest level at the 17(th) dpi followed by a small decline and then stabilized. This increase of DNA concentration suggested recovery of B. bassiana growth in all three substrates. We found that the most suitable killing effectiveness of P. rapae was the 1.0 × 10(7) spores/mL of B. bassiana. In summary, we have established a detection technology that allows a fast and reliable monitoring for the concentration and stability of B. bassiana under different conditions. This technology can benefit and help us in the development of proper management strategies for the application of this biocontrol agent in the field.
作者机构：
[Hou, Maolin; Zhong, Yuqi] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100093, Peoples R China.;[Zhong, Yuqi; Liao, Xiaolan] Hunan Agr Univ, Coll Plant Protect, Changsha 410028, Peoples R China.
通讯机构：
[Maolin Hou] S;State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100093, China<&wdkj&>Author to whom correspondence should be addressed.
关键词：
Cyrtorhinus lividipennis;predation;reproduction;fitness;low temperature storage;biological control
摘要：
Simple Summary Cyrtorhinus lividipennis Reuter (Hemiptera: Miridae) is an important predator of planthoppers and leafhoppers in rice fields. Augmentative biological control has been practiced successfully in many agroecosystems. However, one of the primary obstacles to augmentative biological control is obtaining natural enemies in sufficient numbers and quality when required for release. The development of the low-temperature storage (LTS) technique has been pivotal in ensuring the flexibility and efficiency of the mass production of biological control agents. Here, we measured the effects of LTS on the predatory capacity and reproduction of C. lividipennis adults and the fitness of the F1 generation. The results are expected to improve the successful utilization of the predator in an IPM program. Low-temperature storage (LTS) is a way to adjust natural enemy development to meet field release needs and to protect natural enemies from the odds of long-distance transportation. The mirid bug Cyrtorhinus lividipennis Reuter (Hemiptera: Miridae) is an important predator of planthoppers and leafhoppers in rice fields. In this study, the LTS effects were measured on the predatory capacity and reproduction of the mirid adults (provided with 20% honey solution and stored at 13 degrees C for 12 days), and the fitness of the F1 generation of these adults. Higher predation of the eggs of the brown planthopper Nilaparvata lugens (Stal) (Hemiptera: Delphacidae) was observed in the post-storage females than in the control females. The functional responses of C. lividipennis adults, either exposed to LTS or not, to planthopper eggs fitted well with Holling type II functional responses. Longevity was not affected by LTS, whereas the number of offspring nymphs was 55.6% lower in the post-storage females than in the control females. The fitness of the offspring generation was not affected by the LTS of parental adults. The findings are discussed with their relevance to biological control.
摘要：
<jats:title>Abstract</jats:title><jats:p>Long noncoding RNAs (lncRNAs) are noncoding transcripts that are more than 200 nucleotides long. They play essential roles in regulating a variety of biological processes in many species, including insects, and some lncRNAs have been found to be associated with insecticide resistance. However, the characteristics and biological functions of lncRNAs involved in indoxacarb resistance are unknown in <jats:italic>Spodoptera litura</jats:italic>. We performed RNA sequencing in the SS, InRS, and FInRS of <jats:italic>S. litura</jats:italic> and identified 11978 lncRNAs, including 3136 intergenic lncRNAs, 7393 intronic lncRNAs, and 1449 anti‐sense lncRNAs. Compared with the SS, 51 lncRNAs were upregulated and 134 lncRNAs were downregulated in the two resistant strains, and 908 differentially expressed mRNAs were predicted as the target genes of the 185 differentially expressed lncRNAs. Further analysis showed that 112 of differentially expressed lncRNAs may be associated with indoxacarb resistance by regulating the expression of 14 P450s, seven CCEs, one GST, six UGTs, five ABC transporters, and 24 cuticle protein genes, and 79 of differentially expressed lncRNAs may regulate the expression of 14 detoxification genes and 19 cuticle protein genes to participate in indoxacarb resistance by sponging 10 microRNAs. Interestingly, 47 of differentially expressed lncRNAs may mediate indoxacarb resistance through both lncRNA–mRNA and lncRNA–miRNA–mRNA regulatory pathways. Furthermore, quantitative PCR, RNA interference, and indoxacarb bioassay analyses indicated that overexpressed <jats:italic>LNC_004867</jats:italic> and <jats:italic>LNC_006576</jats:italic> were involved in indoxacarb resistance. This study provides comprehensive information for lncRNAs of <jats:italic>S. litura</jats:italic>, and presents evidence that lncRNAs have key roles in conferring insecticide resistance in <jats:italic>S. litura</jats:italic>.</jats:p>
通讯机构：
[Li Shi; Xiaolan Liao] H;Hunan Provincial Engineering and Technology Research Center for Bio-pesticide and Formulation Processing, College of Plant Protection, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Changsha 410128, China
摘要：
The tobacco cutworm, Spodoptera litura, is an important pest of crop and vegetable plants worldwide, and its resistance to insecticides have quickly developed. However, the resistance mechanisms of this pest are still unclear. In this study, the change in mRNA and miRNA profiles in the susceptible, indoxacarb-resistant and field indoxacarb-resistant strains of S. litura were characterized. Nine hundred and ten co-up-regulated and 737 co-down-regulated genes were identified in the resistant strains. Further analysis showed that 126 co-differentially expressed genes (co-DEGs) (cytochrome P450, carboxy/cholinesterase, glutathione S-transferase, ATP-binding cassette transporter, UDP-glucuronosyl transferase, aminopeptidase N, sialin, serine protease and cuticle protein) may play important roles in indoxacarb resistance in S. litura. In addition, a total of 91 known and 52 novel miRNAs were identified, and 10 miRNAs were co-differentially expressed in the resistant strains of S. litura. Furthermore, 10 co-differentially expressed miRNAs (co-DEmiRNAs) had predicted co-DEGs according to the expected miRNA-mRNA negative regulation pattern and 37 indoxacarb resistance-related co-DEGs were predicted to be the target genes. These results not only broadened our understanding of molecular mechanisms of insecticide resistance by revealing complicated profiles, but also provide important clues for further study on the mechanisms of miRNAs involved in indoxacarb resistance in S. litura.
摘要：
A novel phenazine-l-carboxamide-derived 18-1 (PCND 18-1) was evaluated in terms of its potential for the development and enrichment of new biofungicides for the control of rice sheath blight caused by Rhizoctonia solani. PCND 18-1 exhibited fungicidal activity against R. solani, showing an inhibition rate of 87.64%, with a 50% effective concentration (EC50) 4.25 mu g/mL, regression equation of Y = 0.7105x + 3.8428; and correlation coefficient of 0.9817, which are indicative of its potential as a natural biofungicide. PCND 18-1 also attenuated the pathogenicity of R. solani, which was concentration-dependent. Additionally, the action mode of PCND 18-1 against rice sheath blight (R. solani) was protective better than curative activity under greenhouse condition, as reflected by the corresponding EC50 values of 2.49 and 5.72 mu g/mL. PCND 18-1 can translocate in rice with a low translocation capacity and exhibited a higher capacity for upward (root-leaf) translocation than for downward (leaf-root) translocation. Furthermore, PCND 18-1 demonstrated adhesion to leaves but poor tolerance against rain-washing. The optimal persistence period of PCND 18-1 on rice was 7 d. (C) 2018 Friends Science Publishers
摘要：
<jats:title>Abstract</jats:title><jats:p>Herbivorous attack induces plant defenses. There is evidence that some pests suppress these defenses by interfering with signaling pathways. We here report that infestation by the white-backed planthopper, <jats:italic>Sogatella furcifera</jats:italic>, induces defense responses in rice and infection of the southern rice black-streaked dwarf virus in the planthoppers partially suppresses the planthopper-induced plant defenses. Salicylic acid (SA) levels generally showed a temporal increase pattern while jasmonic acid (JA) levels generally exhibited a decrease pattern in the planthopper-infested plants, irrespective of virus infection status in the insects. The increase in SA was less while the decrease in JA was more in the viruliferous insect-infested plants than in the nonviruliferous insect-infested plants at both 48 and 72 h post infestation. The phytohormone levels corresponded to the patterns of relative expression levels of SA-marker genes (<jats:italic>ICS1</jats:italic> and <jats:italic>NPR1</jats:italic>) and JA-marker gene (<jats:italic>AOS2</jats:italic>) in the plant treatments. Planthoppers performed better on the uninfested plants than on the previously infested plants and were of not significant increase in performance on the plants previously attacked by viruliferous planthoppers in comparison with the plants previously attacked by nonviruliferous insects. Our results indicate that the virus plays a role in partially suppressing the plant defenses induced by the planthopper. These findings provide a new perspective on plant–virus-vector interactions.</jats:p>
