Highly efficient degradation of thidiazuron with Ag/AgCl- activated carbon composites under LED light irradiation
作者：
Yang, Yisi;Zhang, Yan;Dong, Mingguang;Yan, Ting;Zhang, Maosheng;...
期刊：
Journal of Hazardous Materials ,2017年335:92-99 ISSN：0304-3894
通讯作者：
Zeng, Qingru
作者机构：
[Yang, Yisi; Zeng, Qingru] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Yang, Yisi; Zhang, Yan] Huanggang Normal Univ, Hubei Key Lab Proc & Applicat Catalyt Mat, Huanggang, Peoples R China.;[Yang, Yisi; Zhang, Maosheng; Yan, Ting; Dong, Mingguang; Zhang, Yan] Huanggang Normal Univ, Coll Chem Engn, Huanggang 438000, Peoples R China.
通讯机构：
[Zeng, Qingru] H;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
关键词：
Ag/AgCl-activated carbon composites;LED light;Synergistic effect;Thidiazuron
摘要：
Thidiazuron (TDZ;1-phenyl-3-(1,2,3-thiadiazol-5-yl)urea) is one of the most widely used defoliant and easy to dissolve in surface water. Risk associated with the pesticide is not clearly defined, so it is important to remove/degrade TDZ with an efficient and environment friendly technology. Here, we investigated the use of Ag/AgCl-activated carbon (Ag/AgCl–AC) composites in photocatalytic degradation of TDZ under LED light. By the synergic effect of Ag/AgCl and AC, the optimum Ag/carbon weight ratio of 2:1 exhibited superior visible-light photocatalytic activity, the highest removal efficiency was close to 91% in pH 7 matrix. Different types of Ag/AgCl–AC composites were tested, all showed much faster photodegradation kinetics than bare Ag/AgCl in 210 min. The degradation products as identified by HPLC–MS revealed that the hydroxylation by hydroxyl radicals and that of oxidation by superoxide radicals as well as holes were the two main pathways for TDZ degradation. Results revealed that the adsorption concentrated TDZ molecules and the photocatalytically generated radicals rapidly degradated TDZ, the two contributions functioned together for removal of the pollutant from water. ©2017 Elsevier B.V.
语种：
英文
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氮素形态对烟草黑胫病发生的影响
作者：
谭军;周冀衡;李强;张毅;陈丽鹃;...
期刊：
中国烟草科学 ,2017年38(4):80-85 ISSN：1007-5119
作者机构：
[周冀衡; 陈丽鹃; 李强; 张毅; 周路阔; 谭军] 湖南农业大学烟草研究院,湖南农业大学生物科学技术学院,长沙 410128;[周路阔] 湖南省烟草公司郴州市公司,湖南郴州 423000;郴州市桂阳县烟草公司,湖南桂阳,424400;[邓利平] 湖南省桂阳县烟草分公司
关键词：
烟草黑胫病;氮素形态;发病率
摘要：
为寻找控制烟草黑胫病发生的有效措施,研究了硝态氮、铵态氮及其配比液对烟草黑胫病菌孢子萌发、菌丝生长以及烟株次生代谢物和抗病性的影响。结果表明,硝态氮、铵态氮及其混合液均可以加速烟草黑胫病游动孢子的萌发,二者混合溶液更有利于黑胫病菌丝的生长。随着溶液中铵态氮浓度的升高,烟株根系木质素、纤维素和MDA含量以及SOD、POD、 PAL活性显著升高,增强了烟株的抗病性,从而使其发病率和病情指数显著下降。相关性分析表明,烟株黑胫病发病率和病情指数与其根系木质素、MDA含量和SOD、POD、PAL活性均极显著负相关,黑胫病发病率与纤维素含量显著负相关。因此,烟株黑胫病发病率和病情指数与其次生代谢物密切相关,铵态氮可以显著降低烟草黑胫病发病率和病情指数,但硝态氮和铵态氮最适配比及田间抗病效果还需进行大量相关田间试验研究。
语种：
中文
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A Ramie bZIP Transcription Factor BnbZIP2 Is Involved in Drought, Salt, and Heavy Metal Stress Response
作者：
Huang, Chengjian;Zhou, Jinghua;Jie, Yucheng* ;Xing, Hucheng;Zhong, Yingli;...
期刊：
DNA AND CELL BIOLOGY ,2016年35(12):776-786 ISSN：1044-5498
通讯作者：
Jie, Yucheng
作者机构：
[Huang, Chengjian; Zhou, Jinghua; Xing, Hucheng; Liu, Zehang; Ma, Yushen; Yu, Weilin; She, Wei; Zhang, Ying; Jie, Yucheng] Hunan Agr Univ, Inst Ramie, Changsha 410128, Hunan, Peoples R China.;[Huang, Chengjian] Dazhou Inst Agr Sci, Dazhou, Peoples R China.;[Zhou, Jinghua; Xing, Hucheng; Liu, Zehang; Ma, Yushen; She, Wei; Zhang, Ying; Jie, Yucheng] Hunan Agr Univ, Coll Agron, Changsha, Hunan, Peoples R China.;[Xing, Hucheng; Ma, Yushen; Zhong, Yingli; Yu, Weilin; Zhang, Ying; Jie, Yucheng] Hunan Prov Crop Germplasm Innovat & Utilizat Lab, Changsha, Hunan, Peoples R China.;[Zhong, Yingli] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China.
通讯机构：
[Jie, Yucheng] H;Hunan Agr Univ, Inst Ramie, Changsha 410128, Hunan, Peoples R China.
关键词：
Boehmeria nivea;abiotic stress;bZIP;promoter;transgenic plant
摘要：
bZIP transcription factors play key roles in plant growth, development, and stress signaling. A bZIP gene BnbZIP2 (GenBank accession number: KP642148) was cloned from ramie. BnbZIP2 has a 1416 base pair open reading frame, encoding a 471 amino acid protein containing a characteristic bZIP domain and a leucine zipper. BnbZIP2 shares high sequence similarity with bZIP factors from other plants. The BnbZIP2 protein is localized to both nuclei and cytoplasm. Transcripts of BnbZIP2 were found in various tissues in ramie, with significantly higher levels in female and male flowers. Its expression was induced by drought, high salinity, and abscisic acid treatments. Analysis of the cis-elements in promoters of BnbZIP2 identified cis-acting elements involved in growth, developmental processes, and a variety of stress responses. Transgenic Arabidopsis plants' overexpression of BnbZIP2 exhibited more sensitivity to drought and heavy metal Cd stress during seed germination, whereas more tolerance to high-salinity stress than the wild type during both seed germination and plant development. Thus, BnbZIP2 may act as a positive regulator in plants' response to high-salinity stress and be an important candidate gene for molecular breeding of salt-tolerant plants. © 2016 Mary Ann Liebert, Inc.
语种：
英文
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打顶后养分供应水平和采收方式对烤烟钾素积累分配的影响
作者：
邱尧;周冀衡;黄劭理;刘晓颖;李强;...
期刊：
中国烟草学报 ,2016年22(1):42-46 ISSN：1004-5708
通讯作者：
Zhou, J.
作者机构：
[周冀衡; 张毅; 邱尧] 湖南农业大学烟草研究院,长沙410128;[周冀衡; 张毅; 邱尧] 湖南农业大学生物科学技术学院,长沙410128;广西中烟工业有限责任公司,南宁,530001;湖南农业大学烟草研究院,长沙,410128;[黄劭理] 广西中烟工业有限责任公司
通讯机构：
[Zhou, J.] I;Institute of Tobacco, Hunan Agriculture University, Changsha, China
关键词：
打顶;养分;采收方式;烤烟;钾;积累
摘要：
采用砂培的方式,研究了打顶后养分供应水平与叶片采收方式对烤烟体内钾素积累分配的影响。结果表明,钾素在烟株体内移动活跃,自然生长烟株的花序和打顶烟株的上部叶片作为活跃的生长点,是钾素优先分配部位。在低钾条件下,下部叶中贮存的钾素能够大量外运,以满足生长点对钾素的需要;分次采收烟株的下部叶由于离体,其内部固定有大量钾素,故保持较高的钾含量,但花序或上部叶对钾素的需求未得到满足,钾含量偏低:一次采收烟株的下部叶则能够始终充当钾源,有利于上部叶钾含量的提高。保持钾素的正常供应,能够促进烟株生长,增加钾素在烟株体内的积累。
语种：
中文
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枸溶性钾肥与硫酸钾配施对烤烟生长及钾素积累与分配的影响
作者：
李鑫;周冀衡;张毅;宾俊
期刊：
湖南农业大学学报(自然科学版) ,2016年42(5):480-483 ISSN：1007-1032
作者机构：
湖南农业大学烟草研究院, 湖南, 长沙, 410128;湖南农业大学生物科学技术学院, 湖南, 长沙, 410128;[李鑫; 周冀衡; 宾俊] 湖南农业大学烟草研究院, 湖南, 长沙, 410128;[张毅] 湖南农业大学生物科学技术学院, 湖南, 长沙, 410128
关键词：
烟草;枸溶性钾肥;根系性状;根系活力;钾素积累;钾素分配
摘要：
采用砂培方式,研究枸溶性钾肥与硫酸钾配施(质量比1∶3、1∶1和3∶1)对烤烟根系性状、根系活力及烟株钾素吸收积累的影响。结果表明,枸溶性钾肥与硫酸钾配施,可促进烤烟根系生长发育,优于单施硫酸钾和枸溶性钾肥;枸溶性钾肥与硫酸钾配施促进烟株对钾素的吸收和分配,烟株的上、中、下3部位烟叶钾素含量较单施硫酸钾的提高7.60%、23.24%和39.95%,总叶钾含量分配率分别提高8.41%、20.91%和42.03%;以75%枸溶性钾肥与25%硫酸钾配施处理效果最佳。
语种：
中文
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根区温度与氮素形态互作对烟株生物量和烟碱积累的影响
作者：
邱尧;周冀衡;黄劭理;李强;张毅;...
期刊：
烟草科技 ,2015年48(3):19-22 and 52 ISSN：1002-0861
通讯作者：
Zhou, Jiheng
作者机构：
[李强; 张毅; 邱尧; 周冀衡; 刘晓颖; 黄劭理] Institute of Tobacco, Hunan Agriculture University, Changsha, China;[邱尧; 张毅; 周冀衡; 黄劭理] College of Biological Science and Technology, Hunan Agriculture University, Changsha, China
通讯机构：
Institute of Tobacco, Hunan Agriculture University, Changsha, China
关键词：
烟草;根区温度;氮素形态;生物量;烟碱
摘要：
为了揭示根区温度对烤烟烟碱积累的影响机制,通过盆栽试验研究了根区温度(10,18,26和35℃)与氮素形态(100%NH_4~+-N,50%NH_4~+-N+50%NO_3~--N和100%NO_3~--N)互作对烤烟生物量和烟碱积累的影响。结果表明:适当提高根区温度可增加烟株生物量,NO_3~--N比NH_4~+-N更有利于烟株的生长。当根区温度为35℃时,供应100%NH_4~+-N和50%NH_4~+-N+50%NO_3~--N的烟株生长受到抑制,而供应100%NO_3~--N的烟株生长旺盛。氮素形态对烟株生物量的影响大于根区温度。烟株的烟碱积累量在根区温度为18℃时达到最大,供应100%NO_3~--N的烟株烟碱积累量是供应100%NH_4~+-N烟株的1.64倍,施用NO_3~--N有利于烟碱的合成。当根区温度为35℃时,烟株的烟碱合成受到抑制。根区温度对烟碱积累的影响大于氮素形态。
语种：
中文
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不同硫肥施用量对烤烟生长和生理生化指标的影响
作者：
蔡赛男;周冀衡;张毅;张一杨;柳立
期刊：
作物研究 ,2015年(2):170-172 ISSN：1001-5280
作者机构：
[周冀衡; 蔡赛男] 湖南农业大学生物科学技术学院，长沙410128;湖南农业大学烟草工程技术研究中心，长沙410128;湖南农业大学烟草工程技术研究中心,长沙,410128;[周冀衡; 蔡赛男] 湖南农业大学烟草工程技术研究中心，长沙410128;[柳立; 张一杨; 张毅] 湖南农业大学
关键词：
烤烟;硫肥;农艺性状;硝酸还原酶;叶绿素
摘要：
以K326为供试品种,利用盆栽试验研究不同硫肥施用量对烟草农艺性状、叶绿素（叶绿素a、b）含量及硝酸还原酶活性的影响。结果表明:相比于施硫处理,不施硫处理烟株生长状况、烟叶叶绿素含量、硝酸还原酶（NR）活性受到明显的抑制;每株施硫量为1.5 g的中等施硫处理烟株长势最好,移栽前期叶绿素含量及NR活性较高,到成熟期时叶绿素含量及NR活性均降低;每株施硫量为3.0 g的高硫处理,烟株能够完成生育期,但是叶片横向生长受到限制,最大叶宽仅为20.67 cm。高硫处理对烟叶叶绿素含量及NR活性具有显著的促进作用,但是烟株生长后期烟叶中叶绿素含量及NR活性持续偏高,分别为1.10 mg/g和63.10μmol/g·h,不利于烟株的正常成熟落黄及内在品质的形成。
语种：
中文
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根部温度和氮素形态互作对烤烟生长和钾素积累的影响
作者：
邱尧;周冀衡;黄劭理;李强;张毅;...
期刊：
中国烟草学报 ,2015年21(3):88-92 ISSN：1004-5708
通讯作者：
Zhou, Jiheng
作者机构：
[李强; 张毅; 邱尧; 周冀衡; 刘晓颖; 黄劭理] Institute of Tobacco, Hunan Agricultural University, Changsha, China;[邱尧; 张毅; 周冀衡; 黄劭理] College of Biological Science and Technology, Hunan Agricultural University, Changsha, China
通讯机构：
[Zhou, J.] I;Institute of Tobacco, Hunan Agricultural University, Changsha, China
关键词：
根部温度;氮素形态;烤烟;钾素
摘要：
采用盆栽试验,研究了根部不同温度和不同氮素形态的互作对于烤烟生长和钾素积累的影响。结果表明:提高根部温度和供应NO_3-N比例,能够显著促进烟株的生长,根系活力和钾素积累增加。根部温度为10℃和18℃时,供应的NH_4-N烟株根系对钾素的吸收能力较弱;当根部温度为35℃时,供应100%NO_3-N的烟株生长旺盛,但供应NH_4-N的烟株生长受到强烈抑制,根部生物量下降,并有大量的钾素外泌。通过分析可知,氮素形态对烟株生长和钾素积累的影响大于根部温度。提高根部温度和供应硝态氮有利于烟株的生长和钾素的吸收;当根部温度较高时,氨态氮不利于烟株的生长。
语种：
中文
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多种外源性化学物质对烤烟幼苗生长影响及影响差异分析
作者：
李鑫;周冀衡;宾俊;王丰;李强;...
期刊：
核农学报 ,2015年29(12):2425-2432 ISSN：1000-8551
作者机构：
[宾俊; 王丰; 李鑫] 湖南农业大学生物科学技术学院,湖南长沙410128;[宾俊; 王丰; 李鑫] 湖南农业大学烟草研究院,湖南长沙410128;湖南农业大学烟草研究院,湖南长沙,410128;[王丰] 贵州省黔西南州烟草公司,贵州兴义562400;[周冀衡; 李强; 张毅] 湖南农业大学
关键词：
外源性化学物质;烤烟;根系形态结构;光合特性;叶绿素荧光参数
摘要：
为研究烤烟根系分泌物对烤烟幼苗生长的影响,在烤烟幼苗生长过程中施加不同浓度与烤烟根系分泌物主要组成成分相同的3种外源性化学物质(苯甲酸、水杨酸和烟碱),模拟烤烟根系分泌物对烤烟幼苗生长的影响,对比三者的影响差异。结果表明,水杨酸、苯甲酸和烟碱3种物质促进烤烟幼苗根系生长和幼苗光合特性的浓度范围主要集中在0 ~ 200、0 ~ 400、0 ~ 800μmol·L ~(- 1)(其中根表面积达到1 000μmol·L~(- 1),气孔导度和蒸腾速率达到600μmol·L ~(- 1)),促进烤烟幼苗叶绿素荧光参数的浓度范围主要集中在0 ~ 200、0 ~ 800、0 ~ 800μmol·L~(- 1),而3种外源物质的PS Ⅱ实际光合效率在200μmol·L - 1时均达到最大。由此可知,在烤烟幼苗生长过程中,3种外源性化学物质对幼苗的根系形态结构、光合特性和叶绿素荧光参数表现为低浓度促进、高浓度抑制,且浓度越高抑制程度越大,但不同组分对烤烟幼苗生长影响不同,相同浓度下3种物质的促进作用表现为烟碱> 苯甲酸> 水杨酸。本研究可为解决烤烟连作障碍提供理论依据。
语种：
中文
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Isolation of Rhp-PSP, a member of YER057c/YjgF/UK114 protein family with antiviral properties, from the photosynthetic bacterium Rhodopseudomonas palustris strain JSC-3b
作者：
Su, Pin;Feng, Tuizi;Zhou, Xuguo;Zhang, Songbai;Zhang, Yu;...
期刊：
Scientific Reports ,2015年5(1):16121 ISSN：2045-2322
通讯作者：
Zhang, Deyong
作者机构：
[Zhang, Yu; Su, Pin; Zhang, Deyong; Zhang, Zhuo; Cheng, Ju'e; Peng, Jing; Feng, Tuizi; Liu, Yong; Luo, Yuanhua; Zhang, Songbai] Hunan Acad Agr Sci, Hunan Plant Protect Inst, Key Lab Integrated Management Pest & Dis Hort Cro, Changsha 410125, Hunan, Peoples R China.;[Zhou, Xuguo] Univ Kentucky, Dept Entomol, Lexington, KY 40546 USA.;[Lu, Xiangyang] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Zhang, Deyong] H;Hunan Acad Agr Sci, Hunan Plant Protect Inst, Key Lab Integrated Management Pest & Dis Hort Cro, Changsha 410125, Hunan, Peoples R China.
摘要：
Rhodopseudomonas palustris strain JSC-3b isolated from a water canal adjacent to a vegetable field produces a protein that was purified by bioactivity-guided fractionation based on ammonium sulfate precipitation, ion-exchange absorption and size exclusion. The protein was further identified as an endoribonuclease L-PSP (Liver-Perchloric acid-soluble protein) by shotgun mass spectrometry analysis and gene identification, and it is member of YER057c/YjgF/UK114 protein family. Herein, this protein is designated Rhp-PSP. Rhp-PSP exhibited significant inhibitory activities against tobacco mosaic virus (TMV) in vivo and in vitro. To our knowledge, this represents the first report on the antiviral activity of a protein of the YER057c/YjgF/UK114 family and also the first antiviral protein isolated from R. palustris. Our research provides insight into the potential of photosynthetic bacterial resources in biological control of plant virus diseases and sustainable agriculture.
语种：
英文
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Specific adaptation of Ustilaginoidea virens in occupying host florets revealed by comparative and functional genomics
作者：
Zhang, Yong;Zhang, Kang;Fang, Anfei;Han, Yanqing;Yang, Jun;...
期刊：
Nature Communications ,2014年5(1):3849 ISSN：2041-1723
通讯作者：
Sun, Wenxian
作者机构：
[Zhou, Ligang; Fang, Anfei; Sun, Wenxian; Zhang, Kang; Yang, Jun; Han, Yanqing; Peng, You-Liang; Xue, Minfeng; Li, Jianqiang; Zhang, Yong] China Agr Univ, Key Lab Plant Pathol, Minist Agr, Dept Plant Pathol, Beijing 100193, Peoples R China.;[Yang, Jun; Peng, You-Liang; Xue, Minfeng] China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China.;[Bao, Jiandong; Zhou, Bo] Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, State Key Lab Breeding Base Zhejiang Sustainable, Hangzhou 310021, Zhejiang, Peoples R China.;[Hu, Dongwei] Zhejiang Univ, Inst Biotechnol, Hangzhou 310058, Zhejiang, Peoples R China.;[Zhou, Bo] Int Rice Res Inst, Manila 1301, Philippines.
通讯机构：
[Sun, Wenxian] C;China Agr Univ, Key Lab Plant Pathol, Minist Agr, Dept Plant Pathol, Beijing 100193, Peoples R China.
摘要：
Ustilaginoidea virens (Cooke) Takah is an ascomycetous fungus that causes rice false smut, a devastating emerging disease worldwide. Here we report a 39.4a €‰Mb draft genome sequence of U. virens that encodes 8,426 predicted genes. The genome has ∼25% repetitive sequences that have been affected by repeat-induced point mutations. Evolutionarily, U. virens is close to the entomopathogenic Metarhizium spp., suggesting potential host jumping across kingdoms. U. virens possesses reduced gene inventories for polysaccharide degradation, nutrient uptake and secondary metabolism, which may result from adaptations to the specific floret infection and biotrophic lifestyles. Consistent with their potential roles in pathogenicity, genes for secreted proteins and secondary metabolism and the pathogen-host interaction database genes are highly enriched in the transcriptome during early infection. We further show that 18 candidate effectors can suppress plant hypersensitive responses. Together, our analyses offer new insights into molecular mechanisms of evolution, biotrophy and pathogenesis of U. virens. © 2014 Macmillan Publishers Limited. All rights reserved.
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英文
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A classification and regression decision tree method-based model for identifying tobacco growing areas
作者：
张毅;李强;王政;张一扬;周冀衡
期刊：
中国烟草学报 ,2014年20(6):28-33 ISSN：1004-5708
通讯作者：
Zhou, Jiheng
作者机构：
[周冀衡; 张毅] 湖南农业大学生物科学技术学院，湖南省长沙市芙蓉区农大路1号 410128;湖南农业大学烟草研究院，湖南省长沙市芙蓉区农大路1号 410128;湖南农业大学烟草研究院,湖南省长沙市芙蓉区农大路1号,410128;广西中烟工业有限责任公司,广西壮族自治区南宁市北湖南路28号,530001;[周冀衡; 张毅] 湖南农业大学烟草研究院，湖南省长沙市芙蓉区农大路1号 410128
通讯机构：
[Zhou, J.] C;College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, China
关键词：
烤烟;产区;化学指标;识别;分类-回归决策树分析;Mining Tree模型
摘要：
为建立不同产区及风格烤烟烟叶与化学指标之间的关系,对2005~2009年湖南省、河南省、福建省和云南省等4省份共计1040份烟叶样品的21种化学成分进行检测,并利用Mining Tree模型进行分类-回归决策树（C＆RT analysis）数据分析。结果表明：（1）从21个烟叶化学成分中,共筛选出14个特征化学指标,其中钾和硝酸根为4省烟叶共有特征化学指标;（2）湖南、河南、福建和云南4省份识别概率最高的特征化学指标分段组合（Segment）分别为：还原糖（≤24.93%）、钾（〉1.98%）、p H值（≤5.37）（p=0.658）;钾（≤1.98%）、灰分（〉11.03%）、水溶性灰份碱度（≤0.49）、硝酸根（≤0.06%）（p=0.776）;还原糖（〉24.93%）、钾（〉1.98%）、硝酸根（≤0.06%）、蛋白质（〉5.01%）（p=0.914）;钾（≤1.98%）、灰分（≤11.03%）、水溶性总糖（〉28.94%）、硫酸根（≤1.43%）（p=0.957）。分类-回归决策树方法在建立烤烟化学成分识别模型中具有重要的应用价值。
语种：
中文
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Amino acids sensing in vivo, a significant research area on amino acid nutrition
作者：
Wang, Y.;Feng, Z.* ;Zhang, Y.;Jun, F.
期刊：
Journal of Animal and Veterinary Advances ,2014年13(7):460-469 ISSN：1680-5593
通讯作者：
Feng, Z.
作者机构：
Research and Development Center, Twins Group Co., Ltd., Nanchang, 330096 Jiangxi, China;Key Laboratory Agroecol Process Subtropical Region, Chinese Academy of Science, Institute Subtropical Agriculture, Research Center of Healthy Breeding Livestock and Poultry, Furong Road #644, Changsha City, 410125 Hunan, China;Hunan Engineering and Research Center Animal and Poultry Science, Scientific Oberving and Experimetal Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Furong Road #644, Changsha City, 410125 Hunan, China;College of Bioscience and Biotechnology, Hunan Agricultural University, 410126 Hunan Changsha, China
通讯机构：
Key Laboratory Agroecol Process Subtropical Region, Chinese Academy of Science, Institute Subtropical Agriculture, Research Center of Healthy Breeding Livestock and Poultry, Furong Road #644, China
关键词：
Amino acid-sensing;Class C G-protein coupled receptors;Mammalian target of rapamycin (mTOR);Nutrition;SPS-sensing pathway
摘要：
Amino acids are important nutrients that function as both tissue building blocks and metabolic regulators. How amino acid balance is vital for the development and metabolism. Amino acid sensors, serials of particular functional molecules, possess the function of sensing amino acids has been reported in recent years. Here, both intracellular and extracellular amino acid sensors were expounded including their composition, amino acid sensing mechanism and their applications. © Medwell Journals, 2014.
语种：
英文
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Regulation of Transcription of Nucleotide-Binding Leucine-Rich Repeat-Encoding Genes SNC1 and RPP4 via H3K4 Trimethylation
作者：
Xia, Shitou;Cheng, Yu Ti;Huang, Shuai;Win, Joe;Soards, Avril;...
期刊：
PLANT PHYSIOLOGY ,2013年162(3):1694-1705 ISSN：0032-0889
通讯作者：
Li, Xin
作者机构：
[Xia, Shitou] Hunan Agr Univ, Hunan Prov Key Lab Phytohormones, Changsha 410128, Hunan, Peoples R China.;[Cheng, Yu Ti; Huang, Shuai; Xia, Shitou; Li, Xin] Univ British Columbia, Michael Smith Labs, Vancouver, BC V6T 1Z4, Canada.;[Cheng, Yu Ti; Huang, Shuai; Zhang, Yuelin; Li, Xin] Univ British Columbia, Dept Bot, Vancouver, BC V6T 1Z4, Canada.;[Kamoun, Sophien; Jones, Jonathan D. G.; Win, Joe; Soards, Avril] Sainsbury Lab, Norwich NR4 7UH, Norfolk, England.;[Jinn, Tsung-Luo] Natl Taiwan Univ, Dept Life Sci, Taipei 106, Taiwan.
通讯机构：
[Li, Xin] U;Univ British Columbia, Michael Smith Labs, Vancouver, BC V6T 1Z4, Canada.
摘要：
Plant nucleotide-binding leucine-rich repeat (NB-LRR) proteins serve as intracellular sensors to detect pathogen effectors and trigger immune responses. Transcription of the NB-LRR-encoding Resistance (R) genes needs to be tightly controlled to avoid inappropriate defense activation. How the expression of the NB-LRR R genes is regulated is poorly understood. The Arabidopsis (Arabidopsis thaliana) suppressor of npr1-1, constitutive 1 (snc1) mutant carries a gain-of-function mutation in a Toll/Interleukin1 receptor-like (TIR)-NB-LRR-encoding gene, resulting in the constitutive activation of plant defense responses. A snc1 suppressor screen identified modifier of snc1,9 (mos9), which partially suppresses the autoimmune phenotypes of snc1. Positional cloning revealed that MOS9 encodes a plant-specific protein of unknown function. Expression analysis showed that MOS9 is required for the full expression of TIR-NB-LRR protein-encoding RECOGNITION OF PERONOSPORA PARASITICA 4 (RPP4) and SNC1, both of which reside in the RPP4 cluster. Coimmunoprecipitation and mass spectrometry analyses revealed that MOS9 associates with the Set1 class lysine 4 of histone 3 (H3K4) methyltransferase Arabidopsis Trithorax-Related7 (ATXR7). Like MOS9, ATXR7 is also required for the full expression of SNC1 and the autoimmune phenotypes in the snc1 mutant. In atxr7 mutant plants, the expression of RPP4 is similarly reduced, and resistance against Hyaloperonospora arabidopsidis Emwa1 is compromised. Consistent with the attenuated expression of SNC1 and RPP4, trimethylated H3K4marks are reduced around the promoters of SNC1 and RPP4 in mos9 plants. Our data suggest that MOS9 functions together with ATXR7 to regulate the expression of SNC1 and RPP4 through H3K4 methylation, which plays an important role in fine-tuning their transcription levels and functions in plant defense. © 2013 American Society of Plant Biologists. All Rights Reserved.
语种：
英文
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Constitutive expression of cell wall invertase genes increases grain yield and starch content in maize
作者：
Li, Bei;Liu, Hua;Zhang, Yue;Kang, Tao;Zhang, Li;...
期刊：
Plant Biotechnology Journal ,2013年11(9):1080-1091 ISSN：1467-7644
通讯作者：
Zhang, Hongxia
作者机构：
[Liu, Hua; Zhang, Yue; Kang, Tao; Zhang, Hongxia; Li, Bei] Chinese Acad Sci, Shanghai Inst Plant Physiol & Ecol, Natl Key Lab Plant Mol Genet, Shanghai, Peoples R China.;[Zhang, Li] Shandong Agr Univ, Coll Agron, State Key Lab Crop Biol, Tai An, Shandong, Peoples R China.;[Tong, Jianhua; Xiao, Langtao] Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha, Hunan, Peoples R China.
通讯机构：
[Zhang, Hongxia] C;Chinese Acad Sci, Shanghai Inst Plant Physiol & Ecol, Natl Key Lab Plant Mol Genet, Shanghai, Peoples R China.
关键词：
cell wall invertase;GIF1;grain yield;maize;starch content;transgenic plants
摘要：
Grain size, number and starch content are important determinants of grain yield and quality. One of the most important biological processes that determine these components is the carbon partitioning during the early grain filling, which requires the function of cell wall invertase. Here, we showed the constitutive expression of cell wall invertase-encoding gene from Arabidopsis, rice (Oryza sativa) or maize (Zea mays), driven by the cauliflower mosaic virus (CaMV) 35S promoter, all increased cell wall invertase activities in different tissues and organs, including leaves and developing seeds, and substantially improved grain yield up to 145.3% in transgenic maize plants as compared to the wild-type plants, an effect that was reproduced in our 2-year field trials at different locations. The dramatically increased grain yield is due to the enlarged ears with both enhanced grain size and grain number. Constitutive expression of the invertase-encoding gene also increased total starch content up to 20% in the transgenic kernels. Our results suggest that cell wall invertase gene can be genetically engineered to improve both grain yield and grain quality in crop plants. © 2013 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.
语种：
英文
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Mesoporous silica equipped with europium-based chemosensor for mercury ion detection: Synthesis, characterization, and sensing performance
作者：
Zhai Dashun;Zhang Kun;Zhang Yong;Sun Haiyan;Fan Guojing*
期刊：
Inorganica Chimica Acta ,2012年387:396-400 ISSN：0020-1693
通讯作者：
Fan Guojing
作者机构：
[Zhai Dashun; Sun Haiyan] Hunan Agr Univ, Coll Biol Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Fan Guojing] Gannan Normal Univ, Ganzhou 341000, Peoples R China.;[Zhang Kun] Hainan Yangpu Econ Dev Dist, Environm Monitoring Stn, Yangpu 578000, Peoples R China.;[Zhang Yong] Yingtan Municipal Environm Protect Bur, Yingtan 335000, Peoples R China.
通讯机构：
[Fan Guojing] G;Gannan Normal Univ, Ganzhou 341000, Peoples R China.
关键词：
Eu-based;Hg sensor;Luminescence;Silica
摘要：
In this paper, we embed an Eu(III)-based chemosensor into a silica matrix of MCM-41, which is identified and confirmed by IR and small-angle X-ray diffraction spectra. The photophysical property of this composite material is analyzed. It is found that the emission coming from the Eu(III)-based chemosensor is immune to environmental influence due to the unique f-f radiative transitions. The sensing performance of the composite material is then investigated in detail. Data suggest that the composite material exhibits a good linear relationship with increasing Hg(II) concentrations from 1 to 12 μM, with K SV of 1.733 × 10 5 L/mol and linearly dependent coefficient of 0.9942. In addition, the emission of the composite material shows no obvious response towards interference from other metal ions and various pH values, which means that it is a promising chemo-sensing material for Hg(II) detection. © 2012 Elsevier B.V. All rights reserved.
语种：
英文
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Simultaneous silencing of FAD2 and FAE1 genes affects both oleic acid and erucic acid contents in Brassica napus seeds
作者：
Peng, Qi;Hu, Yan;Wei, Ran;Zhang, Yuan;Guan, Chunyun;...
期刊：
Plant Cell Reports ,2010年29(4):317-325 ISSN：0721-7714
通讯作者：
Liu, Chunlin
作者机构：
[Guan, Chunyun; Peng, Qi; Wei, Ran; Hu, Yan; Liu, Chunlin] Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Hunan Prov Inst Oil Crops, Changsha 410128, Hunan, Peoples R China.;[Peng, Qi; Ruan, Ying; Zhang, Yuan] Hunan Agr Univ, Dept Dev Biol, Life Sci & Technol Coll, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liu, Chunlin] H;Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Hunan Prov Inst Oil Crops, Changsha 410128, Hunan, Peoples R China.
关键词：
Brassica napus;Double-gene;Fusion fragment;RNAi;Fatty acid;Modification
摘要：
The fatty acid composition in the seed oil was significantly modified following the introduction of transgenes. To further enhance the desirable characteristics of rapeseed oil, it would be beneficial to develop a new approach for the simultaneous silencing of two or more target genes. Our goals in the current study were to (1) increase oleic acid to more than 75%, (2) reduce polyunsaturated fatty acids (PUFA) to about 10% and erucic acid to zero, and (3) accomplish these changes in a single-transformation event. In a single transformation, two fragments amplified from the fatty acid Δ12-desaturase 2 (BnaFAD2) and fatty acid elongase 1 (BnaFAE1) genes of Brassica napus were linked together to form a fusion fragment. The fusion fragment was then used to assemble unique intron-spliced hairpin interfering constructs. In the transgenic plant FFRP4-4, the expression of BnaFAD2 and BnaFAE1 genes was completely inhibited. The composition of oleic acid in FFRP4-4 rose to 85%, PUFA dropped to 10% and erucic acid was undetectable. All hybrid F1 seeds obtained from the reciprocal crossing of FFRP4-4 and GX-parents (with different genetic backgrounds) contained more than 80% oleic acid, about 10% PUFA and very low, or undetectable, erucic acid. The results confirmed that the fusion fragment silencing construct can simultaneously and effectively silence the target genes on a consistent basis. The strategy provides a useful tool for detecting gene function and advancing genetic engineering techniques for the improvement of agricultural crops. © Springer-Verlag 2010.
语种：
英文
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Transcriptome analysis of the venom glands of the Chinese wolf spider Lycosa singoriensis
作者：
Zhang, Yongqun;Chen, Jinjun;Tang, Xing;Wang, Fan;Jiang, Liping;...
期刊：
Zoology ,2010年113(1):10-18 ISSN：0944-2006
通讯作者：
Liang, Songping
作者机构：
[Rong, Mingqiang; Wang, Meichi; Liu, Zhonghua; Tang, Xing; Liang, Songping; Xiong, Xia; Zhang, Yongqun; Wang, Fan; Jiang, Liping] Hunan Normal Univ, Key Lab Prot Chem & Dev Biol, Coll Life Sci, Changsha 410081, Hunan, Peoples R China.;[Zhang, Yongqun] Tibet Univ, Dept Life Sci, Coll Sci, Lhasa 850000, Peoples R China.;[Chen, Jinjun] Hunan Agr Univ, Coll Biol Sci & Technol, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liang, Songping] H;Hunan Normal Univ, Key Lab Prot Chem & Dev Biol, Coll Life Sci, Changsha 410081, Hunan, Peoples R China.
关键词：
Expressed sequence tag;Spider toxins;Toxin secretion;Toxin sequences
摘要：
The wolf spider Lycosa singoriensis is a hunting spider with a widespread distribution in northwest China. The venom gland of spiders, which is a vent specialized secretory tissue, can secrete abundant and complex toxin components. To extensively examine the transcripts expressed in the venom glands of L. singoriensis, we generated 833 expressed sequence tags (ESTs) from a directional cDNA library. Toxin-like sequences account for 69.1% of these ESTs, 17.3% are similar to cellular transcripts and 13.6% have no significant similarity to any known sequences. Here, we identified 223 novel toxin-like sequences, which can be classified into six different. superfamilies; that means a novel potential source of ligands for varied ion channels was discovered. With the aid of Gene Ontology terms and homology to eukaryotic orthologous groups, the annotation of cellular transcripts revealed some cellular processes important for the toxin secretion of venom glands including protein synthesis, protein folding, tuned post-translational processing and trafficking, etc. (C) 2009 Elsevier GmbH. All rights reserved.
语种：
英文
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Negative Regulation of Systemic Acquired Resistance by Replication Factor C Subunit3 in Arabidopsis
作者：
Xia, Shitou;Zhu, Zhaohai;Hao, Lin;Chen, Jin-Gui;Xiao, Langtao;...
期刊：
PLANT PHYSIOLOGY ,2009年150(4):2009-2017 ISSN：0032-0889
通讯作者：
Li, Xin
作者机构：
[Hao, Lin; Xia, Shitou; Li, Xin] Univ British Columbia, Michael Smith Labs, Vancouver, BC V6T 1Z4, Canada.;[Zhu, Zhaohai; Xia, Shitou; Zhang, Yuelin] Natl Inst Biol Sci, Beijing 102206, Peoples R China.;[Xia, Shitou; Xiao, Langtao] Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth & Dev, Changsha 410128, Hunan, Peoples R China.;[Li, Xin; Chen, Jin-Gui] Univ British Columbia, Dept Bot, Vancouver, BC V6T 1Z4, Canada.
通讯机构：
[Li, Xin] U;Univ British Columbia, Michael Smith Labs, Vancouver, BC V6T 1Z4, Canada.
摘要：
Systemic acquired resistance (SAR) is a plant immune response induced by local necrotizing pathogen infections. Expression of SAR in Arabidopsis (Arabidopsis thaliana) plants correlates with accumulation of salicylic acid (SA) and up-regulation of Pathogenesis-Related (PR) genes. SA is an essential and sufficient signal for SAR. In a genetic screen to search for negative regulators of PR gene expression and SAR, we found a new mutant that is hypersensitive to SA and exhibits enhanced induction of PR genes and resistance against the virulent oomycete Hyaloperonospora arabidopsidis Noco2. The enhanced pathogen resistance in the mutant is Nonexpressor of PR genes1 independent. The mutant gene was identified by map-based cloning, and it encodes a protein with high homology to Replication Factor C Subunit3 (RFC3) of yeast and other eukaryotes; thus, the mutant was named rfc3-1. rfc3-1 mutant plants are smaller than wild-type plants and have narrower leaves and petals. On the epidermis of true leaves, there are fewer cells in rfc3-1 compared with the wild type. Cell production rate is reduced in rfc3-1 mutant roots, indicating that the mutated RFC3 slows down cell proliferation. As Replication Factor C is involved in replication-coupled chromatin assembly, our data suggest that chromatin assembly and remodeling may play important roles in the negative control of PR gene expression and SAR.
语种：
英文
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Expression and characterization of jingzhaotoxin-34, a novel neurotoxin from the venom of the tarantula Chilobrachys jingzhao
作者：
Chen, Jinjun;Zhang, Yongqun;Rong, Mingqiang;Zhao, Liqun;Jiang, Liping;...
期刊：
Peptides ,2009年30(6):1042-1048 ISSN：0196-9781
通讯作者：
Liang, Songping
作者机构：
[Mao, Yucheng; Chen, Jinjun; Rong, Mingqiang; Wang, Meichi; Zhao, Liqun; Liang, Songping; Zhang, Dongyi; Zhang, Yongqun; Jiang, Liping] Hunan Normal Univ, Coll Life Sci, Minist Educ, Key Lab Prot Chem & Dev Biol, Changsha 410081, Hunan, Peoples R China.;[Chen, Jinjun] Hunan Agr Univ, Coll Biol Sci & Technol, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liang, Songping] H;Hunan Normal Univ, Coll Life Sci, Minist Educ, Key Lab Prot Chem & Dev Biol, Changsha 410081, Hunan, Peoples R China.
关键词：
Toxin;Expression;Tetrodotoxin-sensitive sodium channel;Whole-cell recording
摘要：
Jingzhaotoxin-34 (JZTX-34) is a 35-residue polypeptide from the venom of Chinese tarantula Chilobrachys jingzhao. Our previous work reported its full-length cDNA sequence encoding a precursor with 87 residues. In this study we report the protein expression and biological function characterization. The toxin was efficiently expressed by the secretary pathway in yeast. Under whole-cell patch-clamp mode, the expressed JZTX-34 was able to inhibit tetrodotoxin-sensitive (TTX-S) sodium currents (IC50 similar to 85 nM) while having no significant effects on tetrodotoxin-resistant (TTX-R) sodium currents on rat dorsal root ganglion neurons. The inhibition of TTX-S sodium channels was completely reversed by strong depolarization (+120 mV). Toxin treatment altered neither channel activation and inactivation kinetics nor recovery rate from inactivation. However, it is interesting to note that in contrast to huwentoxin-IV, a recently identified receptor site-4 toxin from Ornithoctonus huwena venom, 100 nM JZTX-34 caused a negative shift of steady-state inactivation curve of TTX-S sodium channels by approximately 10 mV. The results indicated that JZTX-34 might inhibit mammalian sensory neuronal sodium channels through a mechanism similar to HWTX-IV by trapping the 1154 voltage sensor in the resting conformation, but their binding sites should not overlay completely. (C) 2009 Elsevier Inc. All rights reserved.
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英文
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