烟田秸秆腐解特性及其腐解液对烟草疫霉菌的影响
作者：
陈丽鹃;陈闺;周冀衡;闫晨兵;柳立;...
期刊：
中国烟草科学 ,2021年42(01):33-39 ISSN：1007-5119
作者机构：
湖南农业大学农学院,生物科学技术学院,长沙 410128;[周冀衡; 柳立; 李强; 陈丽鹃; 张毅; 闫晨兵; 陈闺] 湖南农业大学
关键词：
作物秸秆;腐解特性;养分释放;烟草疫霉菌;抑制作用
摘要：
针对南方常见的烤烟轮作体系,研究3种作物(烟草、油菜和水稻)秸秆的腐解特性及其腐解液对烟草疫霉菌的影响,以期为烟田秸秆的综合利用提供理论依据。采用尼龙网袋法研究了3种秸秆在2种土壤水分条件下的腐解率及养分释放规律,并通过人工制备3种秸秆腐解液,研究了其不同浓度对烟草疫霉菌菌丝生长和孢子囊产生的影响。结果表明,前期烟草秸秆和油菜秸秆腐解较快,但腐解率总体表现为水稻秸秆>油菜秸秆>烟草秸秆,各处理秸秆180 d累积腐解率均在55%以上。3种秸秆的养分释放速率均表现为钾>磷>氮,经过180 d的腐解,氮、磷、钾的释放率分别为60.65%~70.18%、 75.33%~84.81%及93.96%~97.34%。干湿交替处理的秸秆腐解率及养分释放率整体高于淹水处理,但差异均未达到显著水平。 3种秸秆腐解液对烟草疫霉菌菌丝生长和孢子囊产生均有抑制作用,抑制强度表现为水稻秸秆>烟草秸秆>油菜秸秆,且抑菌率随着腐解液浓度的升高而增大,其EC_(50)分别为0.82、1.40和2.08 g/L。因此,烤烟轮作体系中水稻秸秆较易腐解,且3种秸秆腐解液对烟草疫霉菌均存在化感作用,但不同水分条件对秸秆腐解及养分释放效率的影响有待进一步研究。
语种：
中文
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Soil inoculation of Trichoderma asperellum M45a regulates rhizosphere microbes and triggers watermelon resistance to Fusarium wilt
作者：
Zhang, Yi;Tian, Cheng;Xiao, Jiling;Wei, Lin;Tian, Yun* ;...
期刊：
AMB EXPRESS ,2020年10(1):1-13 ISSN：2191-0855
通讯作者：
Tian, Yun;Liang, Zhihuai
作者机构：
[Tian, Yun; Zhang, Yi] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China.;[Xiao, Jiling; Tian, Cheng; Zhang, Yi; Liang, Zhihuai] Hunan Acad Agr Sci, Inst Agr Biotechnol Res, Changsha, Hunan, Peoples R China.;[Wei, Lin] Hunan Acad Agr Sci, Inst Plant Protect, Changsha, Hunan, Peoples R China.
通讯机构：
[Tian, Yun; Liang, Zhihuai] H;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China.;Hunan Acad Agr Sci, Inst Agr Biotechnol Res, Changsha, Hunan, Peoples R China.
关键词：
Trichoderma asperellum M45a;Fusarium wilt;Rhizosphere microorganism;Watermelon
摘要：
Fusarium wilt (FW) caused by Fusarium oxysporum f. sp. niveum (FON) is a soil-borne disease that seriously limits watermelon production. In the present study, Trichoderma asperellum (T. asperellum) M45a was shown to be an effective biocontrol agent against FW. In a pot experiment, the application of 105cfu/g of T. asperellum M45a granules had an improved control effect on FW during the blooming period (up to 67.44%) in soils subjected to five years of continuous cropping with watermelon, while the average length of watermelon vines was also significantly improved (P < 0.05). Additionally, the acid phosphatase (ACP), cellulase (CL), catalase (CAT), and sucrase (SC) activities in the M45a-inoculation group were significantly higher than those in the control (CK) group, and transformation of the soil nutrients (total N, NO3-N, and available P) was significantly increased. Moreover, T. asperellum M45a inoculation reduced fungal diversity, increased bacterial diversity and especiallyenhanced the relative abundance of plant growth-promoting rhizobacteria (PGPR), such as Trichoderma, Sphingomonas, Pseudomonas, Actinomadura, and Rhodanobacter. Through functional prediction, the relative abundance of ectomycorrhiza, endophytes, animal pathotrophs, and saprotrophs in the fungal community was determined to be significantly lower than that observed in the M45a-treated soil. Correlation analysis revealed that Sphingomonas, Pseudomonas, and Trichoderma had the most differences in terms of microorganism abundance, and these differences were positively correlated with ACP, CL, CAT, and SC. These findings provide guidance for the use of fungicides to achieve microecological control of FW in continuously cropped watermelon plots. © 2020, The Author(s).
语种：
英文
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Major Vault Protein Promotes Hepatocellular Carcinoma Through Targeting Interferon Regulatory Factor 2 and Decreasing p53 Activity
作者：
Yu, Haisheng;Li, Mengqi;He, Rui;Fang, Peining;Wang, Qiming;...
期刊：
Hepatology ,2020年72(2):518-534 ISSN：0270-9139
通讯作者：
Liu, Shi
作者机构：
[He, Rui; Zhu, Ying; Peng, Nanfang; Liu, Shi; Fang, Peining; Yu, Haisheng; Li, Mengqi] Wuhan Univ, Coll Life Sci, Modern Virol Res Ctr, State Key Lab Virol, Wuhan, Peoples R China.;[Wang, Qiming] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Peoples R China.;[Yi, Yu] Zhejiang Univ, Life Sci Inst, Minist Educ, Key Lab Biosyst Homeostasis & Protect, Hangzhou, Peoples R China.;[Yi, Yu] Zhejiang Univ, Life Sci Inst, Innovat Ctr Cell Signaling Network, Hangzhou, Peoples R China.;[Wang, Fubing] Wuhan Univ, Zhongnan Hosp, Dept Lab Med, Wuhan, Peoples R China.
通讯机构：
[Liu, Shi] W;Wuhan Univ, Coll Life Sci, State Key Lab Virol, Wuhan 430072, Peoples R China.
关键词：
HDM2;IRF2;Major vault protein (MVP);hepatocellular carcinoma (HCC);p53
摘要：
Background and Aims Major vault protein (MVP) is up-regulated during infections with hepatitis B virus (HBV) and hepatitis C virus (HCV). Here, we found that MVP deficiency inhibited hepatocellular carcinoma (HCC) development induced by diethylnitrosamine, hepatitis B X protein, and HCV core. Approach and Results Forced MVP expression was sufficient to induce HCC in mice. Mechanistic studies demonstrate that the ubiquitin ligase human double minute 2 (HDM2) forms mutual exclusive complexes either with interferon regulatory factor 2 (IRF2) or with p53. In the presence of MVP, HDM2 is liberated from IRF2, leading to the ubiquitination of the tumor suppressor p53. Mouse xenograft models showed that HBV and HCV promote carcinogenesis through MVP induction, resulting in a loss of p53 mediated by HDM2. Analyses of clinical samples from chronic hepatitis B, liver cirrhosis, and HCC revealed that MVP up-regulation correlates with several hallmarks of malignancy and associates with poor overall survival. Conclusions Taken together, through the sequestration of IRF2, MVP promotes an HDM2-dependent loss of p53 that promotes HCC development.
语种：
英文
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Uridine/UMP metabolism and their function on the gut in segregated early weaned piglets
作者：
Li, Guanya;Xie, Chunyan;Wang, Qinhua;Wan, Dan* ;Zhang, Yan;...
期刊：
FOOD & FUNCTION ,2019年10(7):4081-4089 ISSN：2042-6496
通讯作者：
Wan, Dan;Wu, Xin
作者机构：
[Li, Guanya; Wan, Dan; Wu, Xin; Wang, Qinhua; Yin, Yulong] Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Hunan, Peoples R China.;[Li, Guanya; Wan, Dan; Wu, Xin; Wang, Qinhua; Yin, Yulong] Natl Engn Lab Pollut Control & Waste Utilizat Liv, Changsha 410125, Hunan, Peoples R China.;[Li, Guanya; Wan, Dan; Wu, Xin; Wang, Qinhua; Yin, Yulong] Hunan Prov Engn Res Ctr Healthy Livestock & Poult, Changsha 410125, Hunan, Peoples R China.;[Li, Guanya; Wang, Qinhua; Wu, Xin; Yin, Yulong; Xie, Chunyan] Hunan Agr Univ, Hunan Coinnovat Ctr Safety Anim Prod, Changsha 410128, Hunan, Peoples R China.;[Li, Guanya; Wang, Qinhua; Wu, Xin; Yin, Yulong; Xie, Chunyan] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Wan, D; Wu, X] C;[Wan, D; Wu, X] N;[Wan, D; Wu, X; Wu, Xin] H;[Wu, Xin] J;Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Hunan, Peoples R China.
摘要：
Uridine monophosphate (UMP) is a major nucleotide analogue in mammalian milk and uridine (UR) is its gastro-intestinal metabolite in vivo. This study aims to investigate the functional effects of UMP and UR on the gut in vitro and in vivo. Twenty-one piglets were randomly allotted into three groups, the control group, UMP group and UR group, and orally administered UMP or UR for 10 days. Results showed that UMP and UR supplements improved the ADG of piglets, and decreased the diarrhea rate. UR increased the jejunum villus length/crypt depth ratio, Claudin-3 and E-cadherin expression, and the pyrimidine nucleotide metabolic enzymes including CMPK1, RRM2, UPRT, CTPS1 and CTPS2 in the duodenal mucosa. Both the UMP and UR decreased the expression of CAD and RRM2 at the jejunal mucosa. Moreover, UMP and UR increased the apoptosis ratio of intestinal epithelial cells in in vivo and in vitro experiments. Taken together, oral administration of UR and UMP could improve the small intestinal morphology, promote epithelial cell apoptosis and renewal of intestinal villus tips, and benefit intestinal development and health thus improving the growth performance and reducing the risk of diarrhea in early-weaned piglets. © 2019 The Royal Society of Chemistry.
语种：
英文
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Inducible LGALS3BP/90K activates antiviral innate immune responses by targeting TRAF6 and TRAF3 complex
作者：
Xu, Gang;Xia, Zhangchuan;Deng, Feiyan;Liu, Lin;Wang, Qiming;...
期刊：
PLOS Pathogens ,2019年15(8):e1008002 ISSN：1553-7366
通讯作者：
Liu, Shi
作者机构：
[Xu, Gang; Xia, Zhangchuan; Deng, Feiyan; Liu, Lin; Cheng, Zhikui; Zhu, Ying; Liu, Shi] Wuhan Univ, Coll Life Sci, Modern Virol Res Ctr, State Key Lab Virol, Wuhan, Hubei, Peoples R China;[Wang, Qiming] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China;[Yu, Yi] Zhejiang Univ, Life Sci Inst, Key Lab Biosyst Homeostasis & Protect, Minist Educ, Hangzhou, Zhejiang, Peoples R China;[Yu, Yi] Zhejiang Univ, Life Sci Inst, Innovat Ctr Cell Signaling Network, Hangzhou, Zhejiang, Peoples R China;[Wang, Fubing] Wuhan Univ, Dept Lab Med, Zhongnan Hosp, Wuhan, Hubei, Peoples R China
通讯机构：
[Liu, Shi] W;Wuhan Univ, Coll Life Sci, Modern Virol Res Ctr, State Key Lab Virol, Wuhan, Hubei, Peoples R China.
关键词：
Transfection;Influenza A virus;Cytokines;Transcription factors;Reverse transcriptase-polymerase chain reaction;Vesicular stomatitis virus;Viral replication;Viral transmission and infection
摘要：
The galectin 3 binding protein (LGALS3BP, also known as 90K) is a ubiquitous multifunctional secreted glycoprotein originally identified in cancer progression. It remains unclear how 90K functions in innate immunity during viral infections. In this study, we found that viral infections resulted in elevated levels of 90K. Further studies demonstrated that 90K expression suppressed virus replication by inducing IFN and pro-inflammatory cytokine production. Upon investigating the mechanisms behind this event, we found that 90K functions as a scaffold/adaptor protein to interact with TRAF6, TRAF3, TAK1 and TBK1. Furthermore, 90K enhanced TRAF6 and TRAF3 ubiquitination and served as a specific ubiquitination substrate of TRAF6, leading to transcription factor NF-κB, IRF3 and IRF7 translocation from the cytoplasm to the nucleus. Conclusions: 90K is a virus-induced protein capable of binding with the TRAF6 and TRAF3 complex, leading to IFN and pro-inflammatory production. © 2019 Xu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
语种：
英文
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木本泥炭基腐殖酸钾与矿物钾肥配施对酸化植烟土壤养分与烟叶品质的影响
作者：
周杰文;李海平;肖志新;易克;张毅;...
期刊：
江苏农业科学 ,2019年47(8):112-116 ISSN：1002-1302
作者机构：
湖南农业大学生物科学技术学院,湖南长沙,410128;云南省烟草公司保山市公司,云南保山,678000;湖南中烟工业有限责任公司,湖南长沙,410014;云南省保山市隆阳区农业环境保护监测工作站,云南保山,678000
关键词：
植烟酸化土壤;木本泥炭基腐殖酸钾;矿物钾肥;钾素;烟叶品质
摘要：
将木本泥炭基腐殖酸钾与矿物钾肥配施(比例为1:3)用于降低烤烟生产中追肥的用量,研究其对土壤pH值、供钾能力、有效养分含量以及烤后烟叶内在化学成分含量的影响.结果表明,配施土壤调理剂使烟株根际土壤pH值提高了6.71％ ～11.34％.调理剂能够给土壤提高足够的速效钾,配施木本泥炭基腐殖酸钾能够提供酸化植烟土壤的钾素供应强度,在中耕培土时施用调理剂的2个处理土壤中缓效钾含量分别显著提高了9.95％ 、12.88％,提高了土壤钾素库容.相比于常规施肥处理,配施了2种土壤调理剂的T1、T2、T3、T4处理烟株根际土壤有效硫含量分别显著降低了47.00％ 、49.94％ 、47.86％ 、48.39％;土壤调理剂的施用能提高下部烟叶总糖、氧化钾含量,T4处理下部叶总糖含量提升到了31.02％,T3处理下部叶氧化钾含量提升到了5.34％.调理剂的施用还能降低中部烟叶烟碱含量,使其达到我国优质烟叶标准,且氮钾肥的减量不会影响其余烟叶主要化学品质;烟叶产量与均价最优的处理均为T3处理,分别达到了2061 kg/hm2与32.06元/kg.综合土壤养分含量、烟叶品质以及产值、产量分析得出,与常规施肥处理相比较,在降低10％ 总钾施用量的条件下,配施木本泥炭基腐殖酸钾与矿物钾肥(矿物钾肥中耕培土施用),可以有效提高根际土壤pH值以及土壤有效养分供应强度.
语种：
中文
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Isolation of plant growth promoting rhizobacteria and selection of microbial organic fertilizer carriers
作者：
Ding, Mengjiao;Shang, Nianjie;Huang, Ying;Liu, Li;Fan, Wei;...
期刊：
International Journal of Agriculture and Biology ,2019年21(1):77-84 ISSN：1560-8530
通讯作者：
Zhang, Yi;Zhou, Jiheng
作者机构：
[Liu, Li; Zhang, Y; Zhou, Jiheng; Zhang, Yi; Fan, Wei; Ding, Mengjiao] Hunan Agr Univ, Coll Biol Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Shang, Nianjie] Shandong Normal Univ, Coll Life Sci, Jinan 250000, Shandong, Peoples R China.;[Huang, Ying; Peng, Lijuan] Guizhou Univ, Tobacco Coll, Guiyang 550025, Guizhou, Peoples R China.;[Zhou, Zifang] China Jiangsu Ind CO LTD, Nanjing 210019, Jiangsu, Peoples R China.
通讯机构：
[Zhang, Y; Zhou, JH] H;Hunan Agr Univ, Coll Biol Sci & Technol, Changsha 410128, Hunan, Peoples R China.
关键词：
Carrier;Inoculation;Microbial fertilizer;Optimization
摘要：
Plant growth promoting rhizobacteria in microbial fertilizer can not only produce plant hormones to improve the growth of roots and shoots, but also accelerate the release of nutrients in traditional organic fertilizer. Using mixture of plant growth promoting rhizobacteria and traditional organic fertilizer is considered an economical and environment-friendly approach. In this research, a multifunctional composite microbial organic fertilizer was found and tried to solve the problems of soil caused by long-term use of chemical fertilizer and change the irregular release of nutrients in traditional organic fertilizer. Using active microbial concentration and the release of nutrients as indicators to study the effect of the microbial organic fertilizer application on soil fertility, this study indicated that the quantity of active bacteria increased more greatly by turf application than other traditional organic fertilizer carriers. However, using mixed chicken manure (CH) and corn straw (CO) as carriers had same active microbial concentration compared with turf. It indicated that this carrier can be used as an alternative carrier to turf as non-renewable resources. The present findings showed that the CH + CO carrier had the optimal conditions with 30% water content, 30°C temperature, and 108 CFU mL-1 (CFU = Colony-Forming Units) initial cell concentration. The application of the microbial fertilizer was conducive to improving the rhizospheric microecological environment, improving crop yield and quality and alleviating the problems associated with long-term use of chemical fertilizer. © 2019 Friends Science Publishers.
语种：
英文
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Bacillus aciditolerans sp. nov., isolated from paddy soil
作者：
Ding, Meng-Jiao;Shang, Nian-Jie;Xiao, Zhi-Xin;Shao, Fei;Liu, Li;...
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ,2019年69(4):1155-1161 ISSN：1466-5026
通讯作者：
Zhou, Ji-Heng;Zhang, Yi
作者机构：
[Liu, Li; Zhou, Lu-Kuo; Zhou, Ji-Heng; Ding, Meng-Jiao; Zhang, Yi; Zhang, Y] Hunan Agr Univ, Coll Biol Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Shang, Nian-Jie; Shao, Fei] Shandong Normal Univ, Coll Life Sci, Jinan 250014, Shandong, Peoples R China.;[Xiao, Zhi-Xin] Yunnan Prov Tobacco Co Baoshan City Co, Baoshan 678000, Peoples R China.;[Huang, Ying] Guizhou Univ, Tobacco Coll, Guiyang 550025, Guizhou, Peoples R China.;[Zhou, Lu-Kuo] Hunan Prov Tobacco Co Chenzhou City Co, Chenzhou, Peoples R China.
通讯机构：
[Zhou, JH; Zhang, Y] H;Hunan Agr Univ, Coll Biol Sci & Technol, Changsha 410128, Hunan, Peoples R China.
关键词：
Bacillus aciditolerans;average nucleotide;identity;paddy soil;polyphasic taxonomy
摘要：
A Gram-stain-positive, motile, rod-shaped bacterial strain, YN-1(T), was isolated from a rice field in the town of Jietou, Yunnan Province, PR China. Colonies were circular, 1-2 mm in diameter, creamy white, with slightly irregular margins. The isolate grew optimally at 37 degrees C, pH 7.0 and with 1.0 % (w/v) NaCl. On the basis of the results of 16S rRNA gene sequence similarity comparisons, YN-1(T) clustered together with other species of the genus Bacillus and showed highest similarities with Bacillus onubensis 0911MAR22V3(T) (98.0 %), Bacillus humi LMG22167(T) (97.5 %), 'Bacillus timonensis' 10403023 (97.4 %) and 'Bacillussinesaloumensis' P3516 (97.1 %). However, the DNA-DNA hybridization values between YN-1(T) and closely related strains of species of the genus Bacillus were well below 47 %, indicating that they represent different taxa. The average nucleotide identity and the Genome-to-Genome Distance Calculator also revealed low relatedness (below 95 and 70 %, respectively) between YN-1(T) and type strains of closely related species of the genus Bacillus. The DNA G+C content of the strain was 40 mol%. The major cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0, and C16 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, three unidentified aminophospholipids and two other unidentified lipids. Physiological and biochemical test results were also different from those of the most closely related species. On the basis of the phenotypic, genetic and chemotaxonomic data, strain YN-1(T) is considered to represent a novel species of the genus Bacillus, for which the name Bacillusaciditolerans sp. nov. is proposed, with strain YN-1(T) (=CCTCC AB 2017280(T)=JCM 32973(T)) as the type strain.
语种：
英文
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不同类型土壤调理剂对保山烟区酸化土壤改良效果研究
作者：
周杰文;张发明;李海平;肖志新;段志超;...
期刊：
西南农业学报 ,2018年31(2):360-366 ISSN：1001-4829
作者机构：
湖南农业大学生物科学技术学院, 湖南, 长沙, 410128;云南省烟草公司保山市公司, 云南, 保山, 678000;湖南中烟工业有限责任公司, 湖南, 长沙, 410014;[周杰文; 周冀衡; 范伟; 张毅] 湖南农业大学生物科学技术学院, 湖南, 长沙, 410128;[张发明; 李海平; 肖志新] 云南省烟草公司保山市公司, 云南, 保山, 678000
关键词：
土壤调理剂;酸化土壤改良;矿物钾肥;新型增钾型生物有机肥
摘要：
【目的】本文研究了保山烟区土壤酸化的问题。【方法】采用大田试验,选取6种土壤调理剂并以此作为改土措施,研究不同土壤调理剂对酸化植烟土壤化学性质及烤烟品质产量的影响。【结果】除钙镁磷肥外,其它土壤调理剂均能有效提高植烟土壤pH,施用石灰效果最佳,但单施石灰,易产生钙镁钾拮抗及土壤肥力下降的问题;矿物钾肥、新型增钾型生物有机肥能显著增加土壤速效钾、缓效钾、烟叶钾含量,实现增钾提质;0 ~ 15 cm耕层土壤矿物钾肥(水热法)速效钾、缓效钾含量分别极显著高于石灰处理62.13 %、33.97 %,15 ~ 30 cm耕层土壤矿物钾肥(煅烧法)速效钾、缓效钾含量分别极显著高于石灰82.59 %、24.01 %;土壤调理剂主要影响中下部烟叶的化学成分及中上部烟叶的评吸质量;新型增钾型生物有机肥碱解氮含量显著高于对照13.04 %,烤烟产量比对照高286.26 kg /hm~2;新型增钾型生物有机肥和矿物钾肥(水热法)2种土壤改良剂对烟叶化学成分的协调性和烟叶的评吸质量效果最好。【结论】综合改良土壤pH、土壤养分效果和提高烤烟品质、产量几方面,以矿物钾肥(水热法)、新型增钾型生物有机肥效果最佳。
语种：
中文
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The α2δ-1-NMDA Receptor Complex Is Critically Involved in Neuropathic Pain Development and Gabapentin Therapeutic Actions
作者：
Chen, Jinjun;Li, Lingyong;Chen, Shao-Rui;Chen, Hong;Xie, Jing-Dun;...
期刊：
Cell Reports ,2018年22(9):2307-2321 ISSN：2211-1247
通讯作者：
Pan, Hui-Lin
作者机构：
[Zhang, Yuhao; Chen, Jinjun; Zhou, Meng-Hua; Chen, Hong; Pan, Hui-Lin; Xie, Jing-Dun; Li, Lingyong; Chen, Shao-Rui] Univ Texas MD Anderson Canc Ctr, Dept Anesthesiol & Perioperat Med, Ctr Neurosci & Pain Res, Houston, TX 77030 USA.;[Chen, Jinjun] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Xie, Jing-Dun] Sun Yat Sen Univ, Ctr Canc, Dept Anesthesiol, Guangzhou 510060, Guangdong, Peoples R China.;[MacLean, David M.; Jayaraman, Vasanthi; Sirrieh, Rita E.] Univ Texas Hlth Sci Ctr Houston, Dept Biochem & Mol Biol, Houston, TX 77030 USA.
通讯机构：
[Pan, Hui-Lin] U;Univ Texas MD Anderson Canc Ctr, Dept Anesthesiol & Perioperat Med, Ctr Neurosci & Pain Res, Houston, TX 77030 USA.
关键词：
*chronic pain;*dorsal root ganglion;*glutamate;*pregabalin;*presynaptic;*synaptic plasticity;*synaptic trafficking;*synaptic transmission;*thrombospondin;*voltage-gated calcium channels
摘要：
α2δ-1, commonly known as a voltage-activated Ca 2+ channel subunit, is a binding site of gabapentinoids used to treat neuropathic pain and epilepsy. However, it is unclear how α2δ-1 contributes to neuropathic pain and gabapentinoid actions. Here, we show that Cacna2d1 overexpression potentiates presynaptic and postsynaptic NMDAR activity of spinal dorsal horn neurons to cause pain hypersensitivity. Conversely, Cacna2d1 knockdown or ablation normalizes synaptic NMDAR activity increased by nerve injury. α2δ-1 forms a heteromeric complex with NMDARs in rodent and human spinal cords. The α2δ-1-NMDAR interaction predominantly occurs through the C terminus of α2δ-1 and promotes surface trafficking and synaptic targeting of NMDARs. Gabapentin or an α2δ-1 C terminus-interfering peptide normalizes NMDAR synaptic targeting and activity increased by nerve injury. Thus, α2δ-1 is an NMDAR-interacting protein that increases NMDAR synaptic delivery in neuropathic pain. Gabapentinoids reduce neuropathic pain by inhibiting forward trafficking of α2δ-1-NMDAR complexes. Chen et al. show that α2δ-1, through its C terminus, physically interacts with NMDA receptors and promotes synaptic expression of α2δ-1-NMDA receptor complexes in neuropathic pain. Gabapentin reduces neuropathic pain primarily by targeting α2δ-1-bound NMDA receptors. © 2018 The Author(s)
语种：
英文
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Individual components of paired typical NLR immune receptors are regulated by distinct E3 ligases
作者：
Dong, Oliver Xiaoou;Ao, Kevin;Xu, Fang;Johnson, Kaeli C. M.;Wu, Yuxiang;...
期刊：
NATURE PLANTS ,2018年4(9):699-710 ISSN：2055-026X
通讯作者：
Li, Xin
作者机构：
[Dong, Oliver Xiaoou; Chen, Xuejin; Huang, Yan; Liu, Yanan; Li, Xin; Wu, Yuxiang; Xia, Shitou; Xu, Fang; Johnson, Kaeli C. M.; Ao, Kevin] Univ British Columbia, Michael Smith Labs, Vancouver, BC, Canada.;[Dong, Oliver Xiaoou; Huang, Yan; Liu, Yanan; Li, Xin; Xu, Fang; Johnson, Kaeli C. M.; Zhang, Yuelin; Ao, Kevin] Univ British Columbia, Dept Bot, Vancouver, BC, Canada.;[Wu, Yuxiang] Shanxi Agr Univ, Coll Agr, Jinzhong, Peoples R China.;[Li, Lin] Natl Inst Biol Sci, Beijing, Peoples R China.;[Chen, She; Xia, Shitou] Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha, Hunan, Peoples R China.
通讯机构：
[Li, Xin] U;Univ British Columbia, Michael Smith Labs, Vancouver, BC, Canada.;Univ British Columbia, Dept Bot, Vancouver, BC, Canada.
摘要：
In plants and animals, nucleotide-binding leucine-rich repeat (NLR) proteins serve as intracellular immune receptors. Defence signalling by NLRs often requires the formation of NLR heteropairs. Our knowledge of the molecular mechanism regulating this process is limited. In a reverse genetic screen to identify the partner of the Arabidopsis typical NLR, SUPRESSOR OF NPR1, CONSTITUTIVE 1 (SNC1), we discovered three NLRs that are redundantly required for SNC1-mediated defence, which were named SIDEKICK SNC1 1 (SIKIC1), SIKIC2 and SIKIC3. Immunoprecipitation–mass spectrometry analyses revealed that SIKIC2 physically associates with SNC1. We also uncovered that the protein level of SIKIC2 is under the control of two previously uncharacterized redundant E3 ubiquitin ligases MUSE1 and MUSE2. As SNC1 accumulation has previously been shown to be regulated by the E3 ubiquitin ligase SCFCPR1, this report provides evidence that the homeostasis of individual components of partnered typical NLRs is subjected to differential regulation via ubiquitin-mediated protein degradation. © 2018, The Author(s).
语种：
英文
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Study on the migration of Phytophthora nicotianae zoospores in the soil
作者：
Tan, Jun;Li, Qiang* ;Chen, Li-juan;Zhang, Yi-yang;Zhang, Yi;...
期刊：
International Journal of Agriculture and Biology ,2018年20(2):397-403 ISSN：1560-8530
通讯作者：
Li, Qiang
作者机构：
[Tan, Jun; Li, Qiang; Zhou, Lu-kuo; Zhang, Yi-yang; Zhang, Yi; Chen, Li-juan] Hunan Agr Univ, Coll Biol Sci & Technol, Inst Tobacco, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Li, Qiang] H;Hunan Agr Univ, Coll Biol Sci & Technol, Inst Tobacco, Changsha 410128, Hunan, Peoples R China.
关键词：
Adsorption;GFPs;Migration;Phytophthora nicotianae;Zoospores
摘要：
Phytophthora nicotianae causing devastating tobacco black shank in all over the world is a soil-borne biflagellated oomycete plant pathogen. P. nicotianae zoospores are the secondary infection of source in tobacco plants, which can spread rapidly in fields by water currents and on their own swimming in aquatic environments. In order to understand the P. nicotianae zoospore's migration and retention mechanism and provide theoretical basis for effectively preventing its migration in soil, this study deeply investigated the adsorption equilibrium parameters, adsorption kinetics and soil-column infiltration characteristics of P. nicotianae zoospores in soil. It did so by using P. nicotianae marked with green fluorescent protein (GFP) and establishing a corresponding mathematical model to provide a theoretical basis for the effective prevention and control of tobacco black shank. The results showed that with a determination coefficient of 99.61% and an adsorption equilibrium parameter K of 7.206×106 mL/g, the adsorption equilibrium characteristics of P. nicotianae zoospores agreed with the Temkin equation in the soil of a tobacco-rice rotation. The adsorption of zoospores achieved a state of equilibrium in 40 min and then became relatively stable after 40-140 min, and the reversible adsorption constant and reversible desorption constant were 5.634×10-1 s-1 and 3.172×10-8 s-1, respectively. The soil significantly hindered the migration of P. nicotianae zoospores, which makes that zoospores mainly stayed in the soil column that ranged from 0-5 cm. Moreover, the redistribution of the zoospores in the unsaturated soil increased the downward migration of the zoospores. In addition, the redistribution process of zoospores in unsaturated soil does increase their downward migration. © 2018 Friends Science Publishers.
语种：
英文
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保山市烟区植烟土壤pH时空变异特征分析
作者：
周杰文;张发明;李海平;周冀衡;肖志新;...
期刊：
湖北农业科学 ,2018年57(9):37-41 ISSN：0439-8114
作者机构：
湖南农业大学生物科学技术学院;[张发明; 肖志新; 李海平] 云南省烟草公司保山市公司;[段志超] 湖南中烟工业有限责任公司;[周冀衡; 李强; 张毅; 周杰文] 湖南农业大学
关键词：
植烟土壤pH;时空变异特征;地统计学
摘要：
采用传统统计学方法以及地统计学方法结合GIS技术研究了云南省保山市烟区植烟土壤耕层(0~20 cm)pH的时空变异特征.结果表明,2011年保山市烟区植烟土壤pH分布在5.5~6.5的样本占38.3%, 2016年下降至32.0%;土壤pH属中等变异强度;2011年和2016年烟区土壤pH块金值∕基台值分别为40.9%和25.3%,变程分别为41.5 km与47.2 km,具有中等强度的空间相关性;Kriging最优内插法结果表明,保山市烟区植烟土壤pH呈现逐年下降的趋势,下降趋势由西北方向与东南方向逐渐向中部地区减小,中部地区所受影响不大.
语种：
中文
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Conversion of a rice CMS maintainer into a photo- or thermo-sensitive genetic male sterile line
作者：
Tan, Yanning;Sun, Xuewu;Fang, Baohua;Yu, Dong;Sun, Zhizhong;...
期刊：
Molecular Breeding ,2018年38(5):1-11 ISSN：1380-3743
通讯作者：
Duan, Meijuan;Yuan, Dingyang;Zhang, Yongfei
作者机构：
[Duan, Meijuan; Yuan, Dingyang; Yu, Dong; Wang, Weiping; Sun, Xuewu; Duan, MJ; Yuan, DY; Sheng, Xiabing; Sun, Zhizhong; Tan, Yanning] Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha 410125, Hunan, Peoples R China.;[Yuan, Dingyang; Yu, Dong; Wang, Weiping; Sun, Xuewu; Sheng, Xiabing; Sun, Zhizhong; Tan, Yanning] Hunan Acad Agr Sci, Changsha 410125, Hunan, Peoples R China.;[Fang, Baohua] Hunan Rice Res Inst, Changsha 410125, Hunan, Peoples R China.;[Duan, Meijuan; Yuan, Dingyang; Yu, Dong; Sheng, Xiabing; Sun, Zhizhong] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Yin, Xiaolin; Liu, Ling] Hunan Univ, Grad Sch, Longping Branch, Changsha 410125, Hunan, Peoples R China.
通讯机构：
[Duan, MJ; Yuan, DY; Zhang, Yongfei] H;Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha 410125, Hunan, Peoples R China.;Hunan Agr Biotechnol Res Ctr, Changsha 410125, Hunan, Peoples R China.
关键词：
60CO-gamma irradiation;Heterosis utilization;Hybrid rice;Maintainer line;Photoperiod/temperature-sensitive male sterile line
摘要：
A maintainer line of 3-line hybrid rice commonly presents a certain genetic distance to a 2-line restorer line, but in many cases, 2-line restorer lines present defects upon recovery of the object cytoplasmic male sterile (CMS) line of the maintainer line, which impedes the utilization of their heterosis. Here, we report a strategy and an example of converting a maintainer into a photoperiod/temperature-sensitive genic male sterile (P/TGMS) line with an almost identical genetic background, thus maximizing the heterosis. Firstly, through treatment of maintainer line T98B with 60CO-γ irradiation, we identified the TGMS line T98S, which is sterile at higher temperatures and fertile at lower temperatures. Secondly, the T98S line was proven to be identical to T98B with regard to genetic background via an examination of 48 parental polymorphous SSR markers and exhibited excellent blossom traits similar to those of T98B, with an extensive forenoon flowering rate of 75.92% and a high exertion rate of 64.59%. Thirdly, in a combination test, three out of six hybrids from T98S crossed with 2-line restorer lines showed a yield increase of 6.70–15.69% for 2 consecutive years. These results demonstrated that the strategy can generate a new P/TGMS line with strong general combining ability (converted from a maintainer line), thus helping to increase the genetic diversity of male sterile heterotic groups. © 2018, The Author(s).
语种：
英文
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Combining ability of mineral element contents in hybrid rice
作者：
Zhu, Xudong* ;Chen, Hao* ;Zhang, Yangjun* ;Zhao, Ju;Zhou, Qingming;...
期刊：
International Journal of Agriculture and Biology ,2018年20(5):1089-1096 ISSN：1560-8530
通讯作者：
Zhu, Xudong;Chen, Hao;Zhang, Yangjun
作者机构：
[Zhou, Qingming; Zhu, Dan; Zhao, Ju; Zhu, Xudong; Zhu, Ziliang] Hunan Agr Univ, Coll Agron, Southern Reg Collaborat Innovat Ctr Modern Prod, Multiple Copping Syst Paddy Area, Changsha 410128, Hunan, Peoples R China.;[Chen, Hao] Fujian Acad Agr Sci, Inst Crop Sci, Fuzhou 350013, Fujian, Peoples R China.;[Zhang, Yangjun] Dabeinong Co, Beijing 10080, Peoples R China.;[Zhou, Xixin] Hunan Agr Univ, Res Inst New Rural Area, Changsha 410128, Hunan, Peoples R China.;[Liu, Shuangqing] Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Zhu, Xudong] H;[Chen, Hao] F;[Zhang, Yangjun] D;Hunan Agr Univ, Coll Agron, Southern Reg Collaborat Innovat Ctr Modern Prod, Multiple Copping Syst Paddy Area, Changsha 410128, Hunan, Peoples R China.;Fujian Acad Agr Sci, Inst Crop Sci, Fuzhou 350013, Fujian, Peoples R China.
关键词：
Combining ability;Correlation analysis;Heterosis;Mineral elements;Rice
摘要：
Understanding the genetic control of mineral element contents in the last three leaves of hybrid rice will shed light on breeding hybrid rice with ideal mineral element contents. In this study, 25 hybrid rice combinations were created using five elite male sterile lines and five restorer lines. The combining ability of potassium (K), calcium (Ca), iron (Fe), copper (Cu), magnesium (Mg), manganese (Mn), phosphorus (P), sulfur (S) and zinc (Zn) in the last three leaves of these crosses were analyzed at both active tillering stage and milky stage. The results showed that the variance of the general combining ability (GCA) of these parents was larger than the variance of the specific combining ability (SCA) of all crosses. Moreover, larger variation range of heterosis was found in the lines crossed from the parents with the larger variance of SCA. The restorer lines exhibited stronger heritability than sterile lines for Ca, Cu, Mn, S, Zn at active tillering stage and for K, Ca, S, Zn at milky stage. The further phenotypic evaluation for tested crosses revealed that the content of some mineral elements in the last three leaves showed positively significant correlation with yield-related traits. Selecting parents with higher GCA and larger variance of SCA will help us develop the hybrid combination with ideal content of mineral elements and good yield performance for the future hybrid rice breeding program. © 2018 Friends Science Publishers.
语种：
英文
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Elucidating Variations in Nitrogen Requirement According to Yield, Variety and Cropping System for Chinese Rice Production
作者：
Zhang Yi;Chen Xinping;Ma Wenqi;Cui Zhenling*
期刊：
土壤圈(英文版) ,2017年27(2):358-363 ISSN：1002-0160
通讯作者：
Cui Zhenling
作者机构：
[Zhang Yi; Cui Zhenling; Chen Xinping] China Agr Univ, Ctr Resources Environm & Food Secur, Beijing 100193, Peoples R China.;[Zhang Yi] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Ma Wenqi] Hebei Agr Univ, Coll Resources & Environm Sci, Baoding 071001, Peoples R China.
通讯机构：
[Cui Zhenling] C;China Agr Univ, Ctr Resources Environm & Food Secur, Beijing 100193, Peoples R China.
关键词：
grain and straw N concentrations;grain yield;harvest index;N management;plant N uptake;reciprocal internal efficiency
摘要：
Better understanding of the factors that influence crop nitrogen (N) requirement plays an important role in improving regional N recommendations for rice (Oryza sativa L.) production. We collected data from 1 280 plot-level measurements in different reaches of the Yangtze River, China to determine which factors contributed to variability in N requirement in rice. Yield, variety, and cropping system were significantly related to N requirement. The N requirement remained consistent at about 18.6 kg N Mg~(-1) grain as grain yield increased from 7 to 9 Mg ha~(-1), then decreased to 18.1, 16.9,and 15.9 kg N Mg~(-1) grain as yield increased to 9-10, 10-11, and > 11 Mg ha~(-1), respectively. The decreased requirement for N with increasing yield was attributable to declining N concentrations in grain and straw and increased harvest index. Super rice variety had lower N requirement (17.7 kg N Mg~(-1) grain) than ordinary inbred and hybrid varieties (18.5 and 18.3 kg N Mg~(-1) grain, respectively), which was a result of lower grain and straw N concentrations of super rice. The N requirements were 19.2, 17.8, and 17.5 kg N Mg~(-1) grain for early, middle, and late rice cropping systems, respectively. In conclusion, the rice N requirement was affected by multiple factors, including yield, variety, and cropping system, all of which should be considered when planning for optimal N management.
语种：
英文
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Screening and identification of genes associated with flower senescence in tree peony (Paeonia x suffruticosa Andrews) using suppression subtractive hybridization
作者：
Liu, Chunying;Li, Fangzheng;Gai, Shupeng;Zhang, Yuxi;Zhan, Xinmei;...
期刊：
JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY ,2017年92(2):146-154 ISSN：1462-0316
通讯作者：
Zheng, Guosheng;Lu, Xiangyang
作者机构：
[Liu, Chunying; Zhan, Xinmei; Gai, Shupeng; Zheng, Guosheng; Zhang, Yuxi] Qingdao Agr Univ, Coll Life Sci, Key Lab Plant Biotechnol Univ Shandong Prov, Qingdao, Peoples R China.;[Li, Fangzheng] Qingdao Agr Univ, Coll Anim Sci & Technol, Qingdao, Peoples R China.;[Lu, Xiangyang] Hunan Agr Univ, Hunan Agr Bioengn Res Inst, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China.
通讯机构：
[Zheng, Guosheng] Q;[Lu, Xiangyang] H;Qingdao Agr Univ, Coll Life Sci, Key Lab Plant Biotechnol Univ Shandong Prov, Qingdao, Peoples R China.;Hunan Agr Univ, Hunan Agr Bioengn Res Inst, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China.
关键词：
Tree peony;suppression subtractive hybridization;cDNA library;prolong florescence;flower senescence
摘要：
A short blossom period is a major obstacle for the tree peony industry. In order to preferentially isolate genes associated with flower senescence in the tree peony variety ‘Lu He Hong’, forward and reverse suppression subtractive hybridization cDNA libraries were constructed using senescent and blooming petals as testers, respectively. A total of 712 high-quality expressed sequence tags, representing 211 unigenes, were obtained, and 181 unigenes were annotated on the basis of sequence similarity with data in the National Center for Biotechnology Information’s non-redundant database. In addition, 168 unigenes were functionally annotated using Kyoto Encyclopedia of Genes and Genomes databases, and 44 unigenes were classified into 14 functional categories using the Clusters of Orthologous Groups. Most of the unigenes appear to be involved in cellular components, biological processes, and molecular functions, according to Gene Ontology analysis. Analysis of 23 chosen genes using quantitative reverse transcription PCR confirmed that their transcription levels were consistent with the results of the suppression subtractive hybridization libraries. © 2016 The Journal of Horticultural Science & Biotechnology Trust.
语种：
英文
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不同富钾土壤调理剂对植烟土壤供钾特性和烟叶品质的影响
作者：
李海平;丁梦娇;张发明;刘浩;段志超;...
期刊：
中国烟草科学 ,2017年38(4):64-69,75 ISSN：1007-5119
作者机构：
云南省烟草公司保山市公司, 云南, 保山, 678000;湖南农业大学生物科学技术学院, 长沙, 410128;湖南中烟工业有限责任公司, 长沙, 410014;[李海平; 张发明; 肖志新] 云南省烟草公司保山市公司, 云南, 保山, 678000;[丁梦娇; 刘浩; 周冀衡; 范伟; 张毅] 湖南农业大学生物科学技术学院, 长沙, 410128
关键词：
中耕培土;富钾土壤调理剂;不定根;化学品质;钾素
摘要：
为探究在中耕培土期追施富钾土壤调理剂对烤烟生产的影响,利用矿物钾肥(水热法)、木本泥炭基腐植酸钾、矿物钾肥(煅烧法)部分替代追肥中的KNO_3或K_2SO_4,研究其对烤烟根区土壤pH、钾含量及烤烟不定根生物量和烟叶品质的影响。结果表明,在中耕培土期追施富钾土壤调理剂能显著提高采烤结束时烟株根区土壤pH,促进烟株不定根发育及地上部分生物量的增加,效果为矿物钾肥(水热法)>木本泥炭基腐植酸钾>矿物钾肥(煅烧法)。追施矿物钾肥(水热法)及木本泥炭基腐植酸钾能显著提高耕层土壤速效钾(0~15 cm土层)、缓效钾(15~30 cm土层)及中下部烟叶中的钾含量,其0~15 cm土层速效钾含量分别高于对照13.55%、12.10%,15~30 cm土层缓效钾含量分别高于对照19.73%、18.90%,中下部烟叶钾含量分别高于对照21.58%、15.68%。追施矿物钾肥(水热法),中、下部烟叶烟碱含量较对照分别降低21.47%、 17.02%。在酸化植烟土壤上,中耕培土期间追施矿物钾肥(水热法)及木本泥炭基腐植酸钾后能改善土壤供钾能力,改良土壤酸化,提高烤烟不定根生物量及烟叶品质。
语种：
中文
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Highly efficient degradation of thidiazuron with Ag/AgCl- activated carbon composites under LED light irradiation
作者：
Yang, Yisi;Zhang, Yan;Dong, Mingguang;Yan, Ting;Zhang, Maosheng;...
期刊：
Journal of Hazardous Materials ,2017年335:92-99 ISSN：0304-3894
通讯作者：
Zeng, Qingru
作者机构：
[Yang, Yisi; Zeng, Qingru] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Yang, Yisi; Zhang, Yan] Huanggang Normal Univ, Hubei Key Lab Proc & Applicat Catalyt Mat, Huanggang, Peoples R China.;[Yang, Yisi; Zhang, Maosheng; Yan, Ting; Dong, Mingguang; Zhang, Yan] Huanggang Normal Univ, Coll Chem Engn, Huanggang 438000, Peoples R China.
通讯机构：
[Zeng, Qingru] H;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
关键词：
Ag/AgCl-activated carbon composites;LED light;Synergistic effect;Thidiazuron
摘要：
Thidiazuron (TDZ;1-phenyl-3-(1,2,3-thiadiazol-5-yl)urea) is one of the most widely used defoliant and easy to dissolve in surface water. Risk associated with the pesticide is not clearly defined, so it is important to remove/degrade TDZ with an efficient and environment friendly technology. Here, we investigated the use of Ag/AgCl-activated carbon (Ag/AgCl–AC) composites in photocatalytic degradation of TDZ under LED light. By the synergic effect of Ag/AgCl and AC, the optimum Ag/carbon weight ratio of 2:1 exhibited superior visible-light photocatalytic activity, the highest removal efficiency was close to 91% in pH 7 matrix. Different types of Ag/AgCl–AC composites were tested, all showed much faster photodegradation kinetics than bare Ag/AgCl in 210 min. The degradation products as identified by HPLC–MS revealed that the hydroxylation by hydroxyl radicals and that of oxidation by superoxide radicals as well as holes were the two main pathways for TDZ degradation. Results revealed that the adsorption concentrated TDZ molecules and the photocatalytically generated radicals rapidly degradated TDZ, the two contributions functioned together for removal of the pollutant from water. ©2017 Elsevier B.V.
语种：
英文
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Physiological response and comparative proteomic analysis of tobacco seedling roots to NH4+
作者：
Tan, Jun;Li, Qiang* ;Zhou, Ji-heng* ;Chen, Li-juan;Zhang, Yi-yang;...
期刊：
International Journal of Agriculture and Biology ,2017年19(5):1270-1278 ISSN：1560-8530
通讯作者：
Li, Qiang;Zhou, Ji-heng
作者机构：
[Tan, Jun; Li, Qiang; Bin, Jun; Li, Q; Zhou, Ji-heng; Zhang, Yi-yang; Zhang, Yi; Chen, Li-juan] Hunan Agr Univ, Coll Biol Sci & Technol, Inst Tobacco, Changsha 410128, Hunan, Peoples R China.;[Wang, Ruo-zhong] Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Li, Q; Zhou, JH] H;Hunan Agr Univ, Coll Biol Sci & Technol, Inst Tobacco, Changsha 410128, Hunan, Peoples R China.
关键词：
2-ED;Nitrogen species;Proteomics;Roots;Tobacco
摘要：
Plants absorb nitrogen, an essential element for their growth and development, mainly in the form of nitrate or ammonium. These nitrogen species have different effects on crops, little is known about their precise influence at both physiological and proteomic levels. This study investigated these mechanisms, hydroponically grown tobacco seedlings treated with three different ratios of NO3- to NH4+ (10:0, 5:5 or 0:10) for 15 days. The results showed that exposure to high NH4+ concentration affected root morphology and inhibited root development. Moreover, malondialdehyde content and the activity of glutamine synthetase, superoxide dismutase, peroxidase and glutathione S-transferase increased with the concentration of NH4+ in solution. Two-dimensional protein gel analysis showed that 40 protein were differentially expressed between the three treatments. 36 of these proteins were identified using liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). Gene ontology analysis indicated that these proteins were involved in metabolism, energy production, cell defense or signal transduction or structural constituents related pathways. Our results suggest that tobacco seedling roots are subjected to NH4+ toxicity that triggers oxidative stress. Our results help understanding the responses to different nitrogen species and to NH4+ toxicity in roots. © 2017 Friends Science Publishers.
语种：
英文
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