通讯作者:
Fajun Nan<&wdkj&>Daoxin Xie<&wdkj&>Jianbin Yan
作者机构:
[Li, Suhua; Yan, Jianbin; Zhang, Chi] Chinese Acad Agr Sci, Agr Genom Inst Shenzhen,Minist Agr, Shenzhen Key Lab Agr Synthet Biol,Genome Anal Lab, Guangdong Lab Lingnan Modern Agr,Shenzhen Branch, Shenzhen 518124, Peoples R China.;[Li, Suhua; Wang, Fei; Li, Yuwen; Wang, Yupei; Li, Haiou; Xie, Daoxin] Tsinghua Univ, Sch Life Sci, Tsinghua Peking Ctr Life Sci, MOE Key Lab Bioinformat, Beijing 100084, Peoples R China.;[Nan, Fajun; Chen, Linhai] Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China.;[Li, Haiou] Hunan Agr Univ, Coll Biosci & Biotechnol, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha 410128, Peoples R China.;[Wang, Ming] Nanjing Agr Univ, Dept Plant Pathol, Nanjing 210000, Peoples R China.
通讯机构:
[Fajun Nan; Jianbin Yan] S;[Daoxin Xie] M;Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Shenzhen Key Laboratory of Agricultural Synthetic Biology, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518124 China<&wdkj&>For correspondence (e-mail fjnan@simm.ac.cn;daoxinlab@tsinghua.edu.cn;jianbinlab@caas.cn).<&wdkj&>Shanghai Institute of Materia Medica, Chinese Academy of Science, Shanghai, 201203 China<&wdkj&>For correspondence (e-mail fjnan@simm.ac.cn
摘要:
Strigolactones play crucial roles in regulating plant architecture and development, as endogenous hormones, and orchestrating symbiotic interactions with fungi and parasitic plants, as components of root exudates. rac-GR24 is currently the most widely used strigolactone analog and serves as a reference compound in investigating the action of strigolactones. In this study, we evaluated a suite of debranones and found that 2-nitrodebranone (2NOD) exhibited higher biological activity than rac-GR24 in various aspects of plant growth and development in Arabidopsis, including hypocotyl elongation inhibition, root hair promotion and senescence acceleration. The enhanced activity of 2NOD in promoting AtD14-SMXL7 and AtD14-MAX2 interactions indicates that the molecular structure of 2NOD is a better match for the ligand perception site pocket of D14. Moreover, 2NOD showed lower activity than rac-GR24 in promoting Orobanche cumana seed germination, suggesting its higher ability to control plant architecture than parasitic interactions. In combination with the improved stability of 2NOD, these results demonstrate that 2NOD is a strigolactone analog that can specifically mimic the activity of strigolactones and that 2NOD exhibits strong potential as a tool for studying the strigolactone signaling pathway in plants.
通讯机构:
[Xiumei Li; Jun Liu] G;Guangdong Provincial Key Laboratory for Crop Germplasm Resources Preservation and Utilization, Agricultural Biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640 China<&wdkj&>These two authors contributed equally to this work.<&wdkj&>*For correspondence (e-mail liujun@gdaas.cn;lixiumei@agrogene.ac.cn).<&wdkj&>Guangdong Provincial Key Laboratory for Crop Germplasm Resources Preservation and Utilization, Agricultural Biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640 China<&wdkj&>*For correspondence (e-mail liujun@gdaas.cn;lixiumei@agrogene.ac.cn).
摘要:
Poly(ADP-ribose) polymerases, which transfer either monomer or polymer of ADP-ribose from nicotinamide adenine dinucleotide (NAD(+) ) onto target proteins, are required for multiple processes in DNA damage repair, cell cycle, development, and abiotic stress in animals and plants. Here, the uncharacterized rice (Oryza sativa) OsPARP1, which has been predicted to have two alternative OsPARP1 mRNA splicing variants, OsPARP1.1 and OsPARP1.2, was investigated. However, bimolecular fluorescence complementation (BiFC) showed that only OsPARP1.1 interacted with OsPARP3 paralog, suggesting that OsPARP1.1 is a functional protein in rice. OsPARP1 was preferentially expressed in the stamen primordial and pollen grain of mature stamen during flower development. The osparp1 mutant and CRISPR plants were delayed in germination, indicating that defective DNA repair machinery impairs early seed germination. The mutant displayed a normal phenotype during vegetative growth but had a lower seed-setting rate than wild-type plants under normal conditions. Chromosome bridges and DNA fragmentations were detected in male meiocytes at anaphase I to prophase II. After meiosis II, malformed tetrads or tetrads with micronuclei were formed. Meanwhile, the abnormality was also found in embryo sac development. Collectively, these results suggest that OsPARP1 plays an important role in mediating response to DNA damage and gametophyte development, crucial for rice yield in the natural environment.
期刊:
JOURNAL OF THE ELECTROCHEMICAL SOCIETY,2021年168(1):017514 ISSN:0013-4651
通讯作者:
Zhao, Peng;Wu, Zhiyong;Peng, Chang
作者机构:
[Liu, Yanyan; Zhao, Peng; Zou, Xun; Liu, Jiamin; Xie, Ruirui] Southern Med Univ, Sch Pharmaceut Sci, Guangdong Prov Key Lab New Drug Screening, Guangzhou 510515, Guangdong, Peoples R China.;[Wu, Zhiyong] Southern Med Univ, Coll Tradit Chinese Med, Guangzhou 510515, Guangdong, Peoples R China.;[Peng, Chang] Hunan Agr Univ, Coll Sci, Changsha 410128, Peoples R China.
通讯机构:
[Zhao, Peng; Wu, Zhiyong] S;[Peng, Chang] H;Southern Med Univ, Sch Pharmaceut Sci, Guangdong Prov Key Lab New Drug Screening, Guangzhou 510515, Guangdong, Peoples R China.;Southern Med Univ, Coll Tradit Chinese Med, Guangzhou 510515, Guangdong, Peoples R China.;Hunan Agr Univ, Coll Sci, Changsha 410128, Peoples R China.
关键词:
N, P-rGO;electrochemical sensor;hydroquinone;catechol
摘要:
In this paper, the N and P co-doped reduce graphene oxide (N, P-rGO) was prepared with one-pot solution method and characterized by using SEM and electrochemical technology. It was found that the N, P-rGO has an outstanding conductivity, large surface area and excellent electrocatalytic activity to hydroquinone (HQ) and catechol (CC), which usually coexist in aqueous environment. Under the optimal conditions, compared with bare glassy carbon electrode (GCE) and N doped reduce graphene oxide (N-rGO) modified GCE (N-rGO/GCE), the N, P-rGO/GCE displayed an excellent simultaneous determination towards HQ and CC. And the oxidation peak potential difference of HQ and CC obtained from N, P-rGO/GCE was 106 mV, indicating N, P-rGO/GCE has excellent resolution. The obtained detection limit was 62.1 nM and 99.7 nM for HQ and CC in a concentration range of 1 mu M to 100 mu M, respectively. At the same time, N, P-rGO/GCE also displayed satisfied selectivity, decent stability and desirable reproducibility. Furthermore, the fabricated sensor was successfully applied to detect two isomers in tap water and the recoveries of 99.52% to 106.36% and 95.5% to 103.97% for HQ and CC were obtained, which verified the practical application potential of N, P-rGO/GCE.
通讯机构:
[Zhao, Xichen; Ding, Hao] C;CAS Key Laboratory of Agro-ecological Processes in Subtropical Region, Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan 410125, China. and College of Animal Science and Technology, Hunan Agricultural University, Hunan Co-Innovation of Animal Production Safety, Changsha, Hunan 410128, China;CAS Key Laboratory of Agro-ecological Processes in Subtropical Region, Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan 410125, China.
摘要:
Brassica napus L. (oilseed rape) was grown with daikon and white lupin in a polyvinyl chloride split pot experiment (with no barrier between the compartments or by a nylon mesh barrier (37μm) to license partial root interaction, or a solid barrier to stop any root interactions) to examine the effect of rhizosphere interaction on the cadmium uptake. The results showed that shoot and root biomasses of oilseed rape were 40.66% and 26.94% less than that of the monocropped treatment (solid barrier) when intercropping with daikon under the rhizosphere complete interaction. However, the intermingling of roots between oilseed rape and white lupin notably enhanced the dry biomass of oilseed rape by 40.23% and decreased with the reduction of root contact. Oilseed rape intercropping with daikon enhanced the shoot Cd concentration of oilseed rape. The shoot Cd concentration (44.8mg/kg) of oilseed rape when intercropped white lupin under complete rhizosphere interaction were greater than those of other treatments. Additionally, the intermingling of roots played a positive role in the content of citric and malic acids when intercropping with white lupin. In all systems, the BCF values of oilseed rape >5. Therefore, intercropping with white lupin may contribute to higher biomass and increased uptake Cd by oilseed rape. We can toward sustainable positive effects on phytoremediation that based on a better understanding of rhizosphere processes.
期刊:
Journal of Environmental Management,2021年287:112342 ISSN:0301-4797
通讯作者:
Xiao Yang<&wdkj&>Xiulan Yan
作者机构:
[Zhao, Wenchu; Wang, Lingqing; Liang, Tao; Yan, Xiulan; Yang, Xiao] Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Key Lab Land Surface Pattern & Simulat, Beijing 100101, Peoples R China.;[Zhao, Wenchu; Wang, Lingqing; Liang, Tao; Yan, Xiulan; Yang, Xiao] Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100190, Peoples R China.;[Feng, Aixi; Ma, Huansong] Yuhuan Environm Technol Co Ltd, Shijiazhuang 050000, Hebei, Peoples R China.;[Liu, Juan] Guangzhou Univ, Sch Environm Sci & Engn, Inst Environm Res Greater Bay, Minist Educ,Key Lab Water Qual & Conservat Pearl, Guangzhou 510006, Peoples R China.;[Zhou, Yaoyu] Hunan Agr Univ, Coll Resources & Environm, Changsha 410128, Peoples R China.
通讯机构:
[Xiao Yang; Xiulan Yan] K;Key Laboratory of Land Surface Pattern and Simulation, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing, 100101, China<&wdkj&>College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, 100190, China
关键词:
Soil;Dinitrotoluene sulfonate;Distribution;Geochemical transfer;Human health risk assessment
摘要:
The production of 2,4,6-trinitrotoluene (TNT) produces a great deal of waste water, and dinitrotoluene sulfonates (DNTs) are the main pollutants in its waste. This paper presents a pilot investigation on the geochemical transformation of DNTs affected by historical wastewater spillage from a typical TNT production company in Northwest China. In the horizontal direction, DNTs diffused from the evaporation pond to the surrounding area of the site, and the concentration of DNTs in the evaporation pond surface soil exceeded 1000 mg/kg. The horizontal distribution of DNTs in the site showed a migration trend to the east and south of evaporation, which was consistent with the terrain of high northwest and low southeast of the site. Due to the high water solubility of pollutants, water flow is the main driving force for the horizontal distribution of DNTs. In the vertical direction, the concentration of pollutants gradually increased with the depth of the soil. DNTs are mainly adsorbed in the third layer (6.0?8.0 m). It can be seen that the accumulation of the 2,4-DNTs-3-SO3- is obviously larger than that of the 2,4-DNTs-5-SO3- , which may be related to the steric hindrance effect of sulfonic acid groups in the two isomers. Results showed DNTs distribution strongly linked to soil physicochemical properties and the migration of DNTs in soil exhibited obvious heterogeneity in time and space. The carcinogenic risks in surface soil (0?1.5 m) and lower soil (1.5?6.0 m, 6.0?8.0 m) are all higher than 1?10-6; non-carcinogenic risk surface soil (0?1.5 m) is 4.011?10, which is greater than 1, indicating that they may cause certain harm to the human body. Meanwhile, this study presented a pioneering investigation for the contamination and geochemical transfer of DNTs.
摘要:
Previous studies on Bacillus thuringiensis (Bt) resistance in the diamondback moth (DBM) Plutella xylostella have often focused on receptor proteins in the gut. In recent years, many studies have indicated that insect resistance to Bt is related to the immune system. Moreover, our group demonstrated that the expression of peptidoglycan recognition protein (PGRP) genes, which are located upstream of the insect humoral immune system signalling pathway, is significantly different between Cry1Ac-resistant and Cry1Ac-susceptible strains of DBM. Thus, whether antimicrobial peptides (AMPs), which are the major downstream effectors of the insect humoral immune system signalling pathway, are also related to the resistance of DBMs to Bt is a question worthy of in-depth study. In the present study, we sought to (a) clone the CDS of AMP genes expressed in DBMs and (b) explore the effect of the cloned AMPs on the biological characteristics and Bt resistance of DBMs. Here, we revealed three kinds of AMPs (PxDef, PxMor and PxCec), and each of their full-length CDSs was cloned. The recombinant PxDef, PxMor or PxCec proteins, which were expressed in a prokaryotic expression system, have a significant protective effect on DBMs in response to the complex microbial environment of the outside world but have no significant effect on the resistance of DBMs to Cry1Ac toxin. We believe that AMPs are not suitable as the primary entry point for studying the relationship between the immune system of DBMs and their resistance to Bt. This result will enable investigation of breakthrough ideas for further elucidating the mechanism of Bt impact on the insect immune system.
通讯机构:
[Yi Wang] C;Changsha Research Station for Agricultural & Environmental Monitoring, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China<&wdkj&>Key Laboratory for Agro-ecological Processes in Subtropical Regions, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China
关键词:
Catchment environment;Landscape;Water quality;Topography;Soil type;Land use
通讯机构:
[Yu Xiao; Yuanliang Wang] C;College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Hunan Province Key Laboratory of Food Science and Biotechnology, Changsha 410128, China
摘要:
Banana plant height is an important trait for horticultural practices and semi-dwarf cultivars show better resistance to damages by wind and rain. However, the molecular mechanisms controlling the pseudostem height remain poorly understood. Herein, we studied the molecular changes in the pseudostem of a semi-dwarf banana mutant Aifen No. 1 (Musa spp. Pisang Awak sub-group ABB) as compared to its wild-type dwarf cultivar using a combined transcriptome and metabolome approach. A total of 127 differentially expressed genes and 48 differentially accumulated metabolites were detected between the mutant and its wild type. Metabolites belonging to amino acid and its derivatives, flavonoids, lignans, coumarins, organic acids, and phenolic acids were up-regulated in the mutant. The transcriptome analysis showed the differential regulation of genes related to the gibberellin pathway, auxin transport, cell elongation, and cell wall modification. Based on the regulation of gibberellin and associated pathway-related genes, we discussed the involvement of gibberellins in pseudostem elongation in the mutant banana. Genes and metabolites associated with cell wall were explored and their involvement in cell extension is discussed. The results suggest that gibberellins and associated pathways are possibly developing the observed semi-dwarf pseudostem phenotype together with cell elongation and cell wall modification. The findings increase the understanding of the mechanisms underlying banana stem height and provide new clues for further dissection of specific gene functions.
摘要:
The objective of study was to evaluate the effects of energy level in starter ration on blood biochemical parameters, hormone profile, immune response and antioxidant status of calves. A total of eighteen angus calves were divided into three groups in such a way that each group had six calves. Three energy levels were {9 MJ/kg, low metabolizable energy (LME), 12.38 MJ/kg, medium metabolizable energy (MME) and 13.62 MJ/kg, high metabolizable energy (HME)} fed to experimental groups. Results showed that hemoglobin, red blood cells, hematocrit, thrombocytocrit and high-density lipoprotein was higher in HME diet (P<0.05). While, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, mean platelet volume and platelet distribution width was higher in MME diet as compared to HME diet (P<0.05). Results of immunity revealed that IgA content were lowest in calves fed LME diet (P<0.05). Calves received different energy level in the diet had no effect on IgG, IgM and C4 (P>0.05). However, the calves on LME starter diet represented higher blood complement C3 contents (P<0.05). It is concluded that blood metabolites were influenced by energy level in Angus calves.
关键词:
Chicory;growth performance;meat quality;Xiangcun Black pig
摘要:
This study was conducted to explore the effects of dietary supplementation with chicory powder on the growth performance, carcass traits, and meat quality of Xiangcun Black pigs. Forty-five pigs with an average initial body weight of 54.20 +/- 8.70 kg were randomly assigned into one of three groups (15 pigs per group) according to their body weight and litter size. Pigs were fed either a basal diet with no supplement (control group), or a diet supplemented with 5% or 10% chicory powder. Results showed that compared with the control, 10% chicory powder decreased the feed:gain ratio in the first phase (P < 0.01), back fat thickness (P = 0.01), b* value (P = 0.01), and the concentrations of serum total cholesterol and low-density lipoprotein-cholesterin (P < 0.05), but increased the loin-eye area of the longissimus dorsi muscle (LDM, P < 0.05). Chicory also increased the concentrations of several free amino acids and the proportion of n-3 PUFA in the LDM (P < 0.05). In conclusion, these findings indicate that a dietary level of chicory powder up to 10% does not negatively affect performance, instead improving the growth performance, carcass traits, and meat quality of Xiangcun Black pigs.
摘要:
Alfalfa (Medicago sativa L.) is one of the most important forage crops throughout the world. Maximizing leaf retention during the haymaking process is critical for achieving superior hay quality and maintaining biomass yield. Leaf abscission process affects leaf retention. Previous studies have largely focused on the molecular mechanisms of floral organ, pedicel and seed abscission but scarcely touched on leaf and petiole abscission. This study focuses on leaf and petiole abscission in the model legume Medicago truncatula and its closely related commercial species alfalfa. By analyzing the petiolule-like pulvinus (plp) mutant in M. truncatula at phenotypic level (breakstrength and shaking assays), microscopic level (scanning electron microscopy and cross section analyses) and molecular level (expression level and expression pattern analyses), we discovered that the loss-of-function of PLP leads to an absence of abscission zone (AZ) formation and PLP plays an important role in leaflet and petiole AZ differentiation. Microarray analysis indicated that PLP affects abscission process through modulating genes involved in hormonal homeostasis, cell wall remodeling and degradation. Detailed analyses led us to propose a functional model of PLP in regulating leaflet and petiole abscission. Furthermore, we cloned the PLP gene (MsPLP) from alfalfa and produced RNAi transgenic alfalfa plants to downregulate the endogenous MsPLP. Downregulation of MsPLP results in altered pulvinus structure with increased leaflet breakstrength, thus offering a new approach to decrease leaf loss during alfalfa haymaking process.
通讯机构:
[Lixin Wen] C;Colleges of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China<&wdkj&>Hunan Co-innovation Center of Animal Production Safety, Changsha, 410128, China
摘要:
DPANN is known as highly diverse, globally widespread, and mostly ectosymbiotic archaeal superphylum. However, this group of archaea was overlooked for a long time, and there were limited in-depth studies reported. In this investigation, 41 metagenome-assembled genomes (MAGs) belonging to the DPANN superphylum were recovered (18 MAGs had average nucleotide identity [ANI] values of <95% and a percentage of conserved proteins [POCP] of >50%, while 14 MAGs showed a POCP of <50%), which were analyzed comparatively with 515 other published DPANN genomes. Mismatches to known 16S rRNA gene primers were identified among 16S rRNA genes of DPANN archaea. Numbers of gene families lost (mostly related to energy and amino acid metabolism) were over three times greater than those gained in the evolution of DPANN archaea. Lateral gene transfer (LGT; ∼45.5% was cross-domain) had facilitated niche adaption of the DPANN archaea, ensuring a delicate equilibrium of streamlined genomes with efficient niche-adaptive strategies. For instance, LGT-derived cytochrome bd ubiquinol oxidase and arginine deiminase in the genomes of "Candidatus Micrarchaeota" could help them better adapt to aerobic acidic mine drainage habitats. In addition, most DPANN archaea acquired enzymes for biosynthesis of extracellular polymeric substances (EPS) and transketolase/transaldolase for the pentose phosphate pathway from Bacteria. IMPORTANCE The domain Archaea is a key research model for gaining insights into the origin and evolution of life, as well as the relevant biogeochemical processes. The discovery of nanosized DPANN archaea has overthrown many aspects of microbiology. However, the DPANN superphylum still contains a vast genetic novelty and diversity that need to be explored. Comprehensively comparative genomic analysis on the DPANN superphylum was performed in this study, with an attempt to illuminate its metabolic potential, ecological distribution and evolutionary history. Many interphylum differences within the DPANN superphylum were found. For example, Altiarchaeota had the biggest genome among DPANN phyla, possessing many pathways missing in other phyla, such as formaldehyde assimilation and the Wood-Ljungdahl pathway. In addition, LGT acted as an important force to provide DPANN archaeal genetic flexibility that permitted the occupation of diverse niches. This study has advanced our understanding of the diversity and genome evolution of archaea.
通讯机构:
[Qingmei Zhang] H;[Haiyong Wu] S;Hunan Provincial Environmental Protection Air Compound Pollution Prevention Engineering Technology Center, Hunan Research Academy of Environmental Science, Changsha, China<&wdkj&>Soil and Fertilizer Institute of Hunan Province, Hunan Academy of Agricultural Sciences, Changsha, China
摘要:
A Correction to this paper has been published: https://doi.org/10.1007/s10646-021-02412-7