通讯机构：
[Cheng, Tian-Yin] H;Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Research Center for Parasites & Vectors, Hunan Collaborative Innovation Center for Safety Production of Livestock and Poultry, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China.
摘要：
Ticks are hematophagous ectoparasites and cause a major public health threat worldwide. Development of anti-tick vaccines is regarded to be an optimal alternative for tick control. AV422, a unique protein in ticks, is secreted into hosts during blood-feeding, but its roles are not confirmed in Haemaphysalis flava ticks. We retrieved a gene fragment encoding AV422 from a transcriptome dataset of H. flava, and based on it, we reconstructed the full length of AV422 from H. flava (Hf-AV422) by rapid amplification of cDNA ends. Expression profiles of Hf-AV422 in whole ticks and organs of different engorgement levels were determined by qPCR. Then its opening reading frame (ORF) was expressed in Escherichia coli strain BL21 (DE3). The prothrombin time (PT), activated partial thromboplastin time (APTT) and thrombin time (TT) assays were conducted to test anticoagulant activities of the purified recombinant protein (rHf-AV422). The full length of AV422 was 1152 bp. Hf-AV422 showed to be conserved as indicated by multiple sequence alignment. Expression of Hf-AV422 was significantly higher in salivary glands and cuticles than in ovaries. Its expression in whole ticks decreased during engorgement with the highest levels in 1/4 engorged ticks. rHf-AV422 prolonged PT, APTT and TT when incubated with rabbit plasma. Our data demonstrated that Hf-AV422 is a conserved salivary protein with anticoagulant activity. Further studies are needed to test in detail its functional properties to ensure it an adequate antigen candidate for the development of broad-spectrum vaccines against ticks.
通讯机构：
[Jianguo Zeng; Pi Cheng] H;Hunan Key Laboratory of Traditional Chinese Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan 410128, China
摘要：
Riboflavin (RF) and relative derivatives are important coenzyme of some redox enzymes controlling the growing development of plants by harvesting light. The unique isoalloxazine structure unit with special photoredox capacity makes it applicable to induce single electron transfer (SET) oxidation of organic molecules. We report here that riboflavin tetra-acetate (RFTA) catalyzed the oxidation of N-phenyl tetrahydroisoquinoline to alpha-amino C-radical which subsequently undergoes radical type Michael addition to C=C double bond of allylsulfone to access 1-allylated N-phenyl tetrahydroisoquinoline. (C) 2021 Elsevier Ltd. All rights reserved.
摘要：
Toxocara canis causes ocular larva migrans and visceral larva migrans in humans. Knowledge about the molecular mechanism of T. canis-hosts interaction is limited. The proteomic alterations in the plasma of Beagle dogs induced by T. canis infection were studied by the quantitative mass spectrometry-based data-independent acquisition (DIA). 418, 414 and 411 plasma proteins were identified at 24h post-infection (hpi), 96 hpi and 36days post-infection (dpi), including 6, 5 and 23 proteins with differential abundance, respectively. At 24 hpi, the altered proteins, retinoic acid receptor responder protein 2 (RARRES2), WD repeat-containing protein 1 (WDR1), moesin and filamin-A, may participate in pro-inflammatory reaction or promote larvae migration. At 96 hpi, the altered protein C and fibroleukin may maintain the stability of the coagulation system to protect the lung. At 36 dpi, the alterations of C-reactive protein (CRP), ficolin (FCN), complement factor H-related protein 5 (CFHR5) and other complements can affect the three traditional complement system, including the classic pathway, lectin pathway and alternative pathway. These proteins may play important roles in the interaction between T. canis and its definitive hosts. Further study on these altered proteins triggered by T. canis infection may discovery novel therapeutic or diagnostic targets for toxocariasis. SIGNIFICANCE OF THE STUDY: Toxocara canis is one of the globally distributed soil-transmitted helminths, which causes ocular larva migrans and visceral larva migrans in humans and a wide range of warm-blooded animals. T. canis adapts to different microenvironments by resisting and adjusting various biological processes of the hosts. Knowledge about the molecular mechanism of T. canis-hosts interaction is limited. Plasma proteins are good marker for monitoring the occurrence and development of diseases. The proteomic alterations in the plasma of Beagle dogs induced by T. canis infection were studied by the quantitative mass spectrometry-based data-independent acquisition (DIA) in this study. A total of 418, 414 and 411 plasma proteins were identified at 24h post-infection (hpi), 96 hpi and 36days post-infection, respectively. Ten protein with differential abundances were validated by using parallel reaction monitoring (PRM). Collectively, our deep proteomic analysis of plasma revealed that proteins alterations were affected by disease development, and proteomic analysis is an ideal method for quantifying changes in circulating factors on a global scale in response to pathophysiological perturbations such as T. canis infection.
摘要：
The existing literature on the environmental Kuznets curve (EKC) fails to investigate the spatial attribute of the “pollution halo” effect or to integrate the “pollution haven” effect. This paper extends the EKC model to the spatial Durbin model by exploring the spatial spillover of disembodied technology and the pollution transfer effect of environmental regulation. Taking Chinese agricultural foreign trade as an example, our paper finds that the technology spillover does not bring a pollution halo effect to local areas but to adjacent areas. The pollution haven effect impacts the EKC’s turning point, which can be divided into two curves in the spatial dimension: an inverted U-shaped curve, in which the turning point represents the effect of technological innovation, and a U-shaped curve, in which the turning point represents the transfer effect of pollution-intensive industries. Based on the conclusion, this paper recommends that agricultural non-point pollution reduction should be nationally coordinated to establish transport infrastructure and the agricultural human capital conditions for the spatial diffusion of disembodied technology.
作者机构：
[Peng, Zhen; He, Shoupu; Du, Xiongming; Pang, Baoyin; Dai, Panhong; Pan, Zhaoe; Hu, Daowu; Gong, Wenfang; Jia, Yinhua; Fu, Guoyong; Wang, Liru; Geng, Xiaoli; Wang, Liyuan; Chen, Baojun] Chinese Acad Agr Sci, Inst Cotton Res, State Key Lab Cotton Biol, Anyang, Peoples R China.;[Sun, Gaofei] Anyang Inst Technol, Sch Comp Sci & Informat Engn, Anyang, Peoples R China.;[Shi, Weijun; Wang, Junduo] Xinjiang Acad Agr Sci, Res Inst Econ Crops, Urumqi, Peoples R China.;[Xiao, Songhua] Jiangsu Acad Agr Sci, Inst Ind Crops, Nanjing, Peoples R China.;[Cui, Shufang] Hebei Acad Agr & Forestry Sci, Inst Cotton, Shijiazhuang, Hebei, Peoples R China.
通讯机构：
[Du, Xiongming] S;State Key Laboratory of Cotton Biology, Institute of Cotton Research of the Chinese Academy of Agricultural Sciences, Anyang, China
摘要：
Genomic variations in 3,248 tetraploid cotton germplasms and multi-environmental genome-wide association analyses provide insights into the basis of geographic differentiation and fiber improvement in cultivated cotton. Large-scale genomic surveys of crop germplasm are important for understanding the genetic architecture of favorable traits. The genomic basis of geographic differentiation and fiber improvement in cultivated cotton is poorly understood. Here, we analyzed 3,248 tetraploid cotton genomes and confirmed that the extensive chromosome inversions on chromosomes A06 and A08 underlies the geographic differentiation in cultivated Gossypium hirsutum. We further revealed that the haplotypic diversity originated from landraces, which might be essential for understanding adaptative evolution in cultivated cotton. Introgression and association analyses identified new fiber quality-related loci and demonstrated that the introgressed alleles from two diploid cottons had a large effect on fiber quality improvement. These loci provided the potential power to overcome the bottleneck in fiber quality improvement. Our study uncovered several critical genomic signatures generated by historical breeding effects in cotton and a wealth of data that enrich genomic resources for the research community.
期刊：
Journal of Hazardous Materials,2021年411:125094 ISSN：0304-3894
通讯作者：
Qingyun Yan
作者机构：
[Yan, Xizhe; Yan, Qingyun; Weng, Wanlin; Peng, Tao; Shu, Longfei; Xu, Kui; He, Zhili; Yu, Huang; Zheng, Xiafei; Xiao, Fanshu; Liu, Xingyu; Wang, Cheng; Chen, Pubo; Zhong, Qiuping] Sun Yat Sen Univ, Southern Marine Sci & Engn Guangdong Lab Zhuhai, Sch Environm Sci & Engn, Environm Microbi Res Ctr, Guangzhou 510006, Peoples R China.;[Yang, Tony] Agr & Agri Food Canada, Swift Current Res & Dev Ctr, Swift Current, SK S9H 3X2, Canada.;[He, Zhili] Hunan Agr Univ, Coll Agron, Changsha 410128, Peoples R China.
通讯机构：
[Qingyun Yan] E;Environmental Microbiomics Research Center, School of Environmental Science and Engineering, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Sun Yat-sen University, Guangzhou 510006, China
摘要：
The impacts of metal(loids) on soil microbial communities are research focuses to understand nutrient cycling in heavy metal-contaminated environments. However, how antimony (Sb) and arsenic (As) contaminations synergistically affect microbially-driven ecological processes in the rhizosphere of plants is poorly understood. Here we examined the synergistic effects of Sb and As contaminations on bacterial, archaeal and fungal communities in the rhizosphere of a pioneer plant (Miscanthus sinensis) by focusing on soil carbon and nitrogen cycle. High contamination (HC) soils showed significantly lower levels of soil enzymatic activities, carbon mineralization and nitrification potential than low contamination (LC) environments. Multivariate analysis indicated that Sb and As fractions, pH and available phosphorus (AP) were the main factors affecting the structure and assembly of microbial communities, while Sb and As contaminations reduced the microbial alpha-diversity and interspecific interactions. Random forest analysis showed that microbial keystone taxa provided better predictions for soil carbon mineralization and nitrification under Sb and As contaminations. Partial least squares path modeling indicated that Sb and As contaminations could reduce the carbon mineralization and nitrification by influencing the microbial biomass, alpha-diversity and soil enzyme activities. This study enhances our understanding of microbial carbon and nitrogen cycling affected by Sb and As contaminations.
摘要：
Simple Summary MicroRNAs (miRNAs) have been reported with potential regulatory roles in spermatogenesis. In the present study, we demonstrated that miR-126 can stimulate cell proliferation and restrain the apoptosis of immature porcine Sertoli cells by targeting the PIK3R2 gene. Through this process, miR-126 further activates the PI3K/AKT signaling pathway. These results indicated that miR-126, PIK3R2, and the PI3K/AKT signaling pathway might play pivotal regulatory roles in porcine spermatogenesis by deciding the destiny of immature Sertoli cells. The quantity of Sertoli cells in the adult testis decides the daily gamete formation, and accumulating evidence indicates that epigenetic factors regulate the proliferation of Sertoli cells. Research on the function and regulatory mechanism of microRNAs (miRNAs) in Sertoli cells has not been comprehensive yet, especially on domestic animals. In this article, we report that miR-126 controls the proliferation and apoptosis of immature porcine Sertoli cells based on previous studies. Our results confirmed that miR-126 elevation promotes cell cycle progression, cell proliferation and represses cell apoptosis; on the contrary, the inhibitory effects of miR-126 result in the opposite. The phosphoinositide-3-kinase regulatory subunit 2 (PIK3R2) gene, a member of the PI3K family, was verified as a direct target of miR-126 using the dual-luciferase reporter analysis. miR-126 negatively regulated the mRNA and protein expression level of PIK3R2 in immature porcine Sertoli cells. siRNA-induced PIK3R2 inhibition caused similar effects as miR-126 overexpression and eliminated the influences of miR-126 knockdown in immature porcine Sertoli cells. In addition, both miR-126 overexpression and PIK3R2 inhibition elevated the phosphorylation of PI3K and AKT, whereas the miR-126 knockdown demonstrated the contrary result. In short, miR-126 controls the proliferation and apoptosis of immature porcine Sertoli cells by targeting the PIK3R2 gene through the PI3K/AKT signaling pathway. The research supplies a theoretical and practical foundation for exploring the functional parts of miR-126 in swine sperm by defining the destiny of immature Sertoli cells.
关键词：
Pseudorabies virus (PRV);Diagnosis methods;Prevalence;Multiple-species infection;Prevention and control measures
摘要：
Pseudorabies (PR), also called Aujeszky’s disease, is a highly infectious disease caused by pseudorabies virus (PRV). Without specific host tropism, PRV can infect a wide variety of mammals, including pig, sheep, cattle, etc., thereby causing severe clinical symptoms and acute death. PRV was firstly reported in China in 1950s, while outbreaks of emerging PRV variants have been documented in partial regions since 2011, leading to significant economic losses in swine industry. Although scientists have been devoting to the design of diagnostic approaches and the development of vaccines during the past years, PR remains a vital infectious disease widely prevalent in Chinese pig industry. Especially, its potential threat to human health has also attracted the worldwide attention. In this review, we will provide a summary of current understanding of PRV in China, mainly focusing on PRV history, the existing diagnosis methods, PRV prevalence in pig population and other susceptible mammals, molecular characteristics, and the available vaccines against its infection. Additionally, promising agents including traditional Chinese herbal medicines and novel inhibitors that may be employed to treat this viral infection, are also discussed.
作者机构：
[He, Zhenhui] Hunan Univ Changsha, Foreign Languages Dept, Changsha 410082, Hunan, Peoples R China.;[He, Zhenhui] Hunan Agr Univ Changsha, Foreign Languages Dept, Changsha 410128, Hunan, Peoples R China.
摘要：
Automatic marking of English compositions is a rapidly developing field in recent years. It has gradually replaced teachers' manual reading and become an important tool to relieve the teaching burden. The existing literature shows that the error of verb consistency and the error of verb tense are the two types of grammatical errors with the highest error rate in English composition. Hence, the detection results of verb errors can reflect the practicability and effectiveness of an automatic reading system. This paper proposes an English verb's grammar error detection algorithm based on the cyclic neural network. Since LSTM can effectively retain the valid information in the context during training, this paper decided to use LSTM to model the labeled training corpus. At the same time, how to convert the text information in English compositions into numerical values for subsequent calculation is also an important step in automatic reading. Most mainstream tools use the word bag model, i.e., each word is encoded according to the order of each word in the dictionary. Although this encoding method is simple and easy to use, it not only causes the vector to lose the sequence information of the text but also is prone to dimensional disaster. Therefore, word embedding model is adopted in this paper to encode the text, and the text information is sequentially mapped to a low-dimensional vector space. In this way, the position information of the text is not lost, and the dimensional disaster is avoided. The proposed work collects some corpus samples and compares the proposed algorithm with Jouku and Bingguo. The verification results show the superiority of the proposed algorithm in verb error detection.
作者机构：
[Huang, X. H.; Liu, M. S.; Xu, H. Y.] Cent South Univ Forestry & Technol, Coll Life Sci & Technol, Changsha 410004, Peoples R China.;[Zhu, F.] Hunan Agr Univ, Coll Landscape Architecture & Art Design, Changsha 410128, Peoples R China.;[Wang, R. J.] Guangxi Zhuang Autonomous 11 Reg Forestry Res Ins, Guangxi Key Lab Super Trees Resource Cultivat, Nanning, Peoples R China.
关键词：
energy conversion efficiency;heavy metal stress;JIP-test;OJIP curve
摘要：
Manganese (Mn) excess is a major abiotic stress for plant growth. In the present study, we investigated the effects of Mn on photosynthesis in Ligustrum lucidum and Melia azedarach leaves using chlorophyll fluorescence transients. Both plant species were exposed to two Mn concentrations (0.5 and 1 mmol) for 10 and 30 d. Results showed that excess Mn significantly inhibited photosynthesis. With the increase of Mn concentration and stress time, the inhibition was more serious. Mn stress impaired PSII at the donor and the acceptor side by damaging the oxygen-evolving complex and limiting electron transport downstream of QA in both trees. A significant decline in 820 nm reflection curve absorption was observed in M. azedarach, suggesting that the oxidization-reduction reactions in PSI were inhibited but this phenomenon was not observed in L. lucidum. Therefore, excess Mn impaired the whole electron transport chain associated with inactive PSII reaction centers in Ligustrum lucidum and inhibited oxidization-reduction reactions in PSI in M. azedarach.
摘要：
Aspergillus aculeatus ZC-1005 (ZC-1005 was used as the abbreviation of this strain) is a hemicellulase-producing strain isolated from rotten citrus rind buried in the soil. Our previous study has shown its biochemical properties including high xylanase activity, mannanase activity, and degradation reaction with citrus mesocarp. In this study, we focused more on the enzyme safety evaluation and the genome sequencing via PacBio and Illumina platforms. High biological safety of the crude enzymes of ZC-1005 has been proven by the acute oral toxicity test, sub-chronic toxicity test, micronucleus test, and sperm malformation test. The genome of ZC-1005 had a GC content of 52.53%, with a size of 35,458,484 bp, and encoded 10,147 genes. Strain ZC-1005 harbored 269 glycosyl hydrolase (GH) genes of 64 families. The fungus produces cellulose-acting (GH3, GH5, GH12, and GH1) and hemicellulose-acting enzymes (GH16, GH31, GH2, and GH92). In genome annotation, we paid more attention to the genes encoding xylanase, such as gene 01512, gene 05833, gene 05469, gene 07781, gene 08432, gene 09042, gene 08008, and gene 09694. The collaboration between complete genome information and the degradation test confirmed that ZC-1005 could degrade cellulose and xylan. Our results showed that the citrus enzymatic decapsulation technology was efficacious and safe for canned citrus product processing, which may also solve the industrial waste problem. Therefore, ZC-1005 and the crude enzyme secreted from the strain were very promising to be used in the citrus processing industry.
通讯机构：
[Langtao Xiao] C;College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, China<&wdkj&>Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha, China
关键词：
Magnetic solid phase extraction;In situ derivatization;Oilseeds;Phytohormones;Ultra-high performance liquid;chromatography-tandem mass spectrometry
摘要：
Magnetic solid phase extraction integrated with in situ derivations for the profiling of 12 phytohormones in a single rapeseed seed was developed by using ultra-high performance liquid chromatography-tandem mass spectrometry. The Fe3O4@Ti3C2@beta-cyclodextrin nanoparticles were firstly synthesized and used as an adsorbent for the solid-phase extraction of phytohormones. The magnetic dispersive solid-phase extraction and in situ derivation by the addition of N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide were ingeniously combined. This efficient pre-treatment method integrated the extraction, purification, and derivatization processes into one single step. Satisfactory methodological performance was achieved by optimization of the parameters. Linearities (R-2 > 0.9928) and recoveries (80.4 %-115.1%) at three spiked levels, as well as the low matrix effect (from -16.63% to 17.06%) and limits of detection (0.89-13.62 pg/mL) were obtained. The spatio-temporal profiling of target phytohormones in different tissues of rapeseed germination was investigated. This method was successfully employed for analyzing target phytohormones in different oilseeds samples.
摘要：
Camellia sinensis cv. 'Yanling Huayecha' (YHC) is an albino-green chimaeric tea mutant with stable genetic traits. Here, we analysed the cell ultrastructure, photosynthetic pigments, amino acids, and transcriptomes of the albino, mosaic, and green zones of YHC. Well-organized thylakoids were found in chloroplasts in mesophyll cells of the green zone but not the albino zone. The albino zone of the leaves contained almost no photosynthetic pigment. However, the levels of total amino acids and theanine were higher in the albino zone than in the mosaic and green zones. A transcriptomic analysis showed that carbon metabolism, nitrogen metabolism and amino acid biosynthesis showed differences among the different zones. Metabolite and transcriptomic analyses revealed that (1) downregulation of CsPPOX1 and damage to thylakoids in the albino zone may block chlorophyll synthesis; (2) downregulation of CsLHCB6, CsFdC2 and CsSCY1 influences chloroplast biogenesis and thylakoid membrane formation, which may contribute to the appearance of variegated tea leaves; and (3) tea plant variegation disrupts the balance between carbon and nitrogen metabolism and promotes the accumulation of amino acids, and upregulation of CsTSⅠ and CsAlaDC may enhance L-theanine synthesis. In summary, our study provides a theoretical basis and valuable insights for elucidating the molecular mechanisms and promoting the economic utilization of variegation in tea.
