通讯机构：
[Wan, FC ; Liu, L ] H;Hunan Agr Univ, Coll Anim Sci & Technol, Changsha, Hunan, Peoples R China.;Hunan Agr Univ, Coll Vet Med, Changsha, Hunan, Peoples R China.
关键词：
L-leucine;beef cattle;in vitro technique;rumen protection rate;rumen fermentation;bacterial community
摘要：
This study was conducted to compare the effects of rumen-protected (RP-Leu) and unprotected L-leucine (RU-Leu) on the fermentation parameters, bacterial composition, and amino acid metabolism in vitro rumen batch incubation. The 5.00 g RP-Leu or RU-Leu products were incubated in situ in the rumen of four beef cattle (Bos taurus) and removed after 0, 2, 4, 6, 12, 16, and 24 h to determine the rumen protection rate. In in vitro incubation, both RP-Leu and RU-Leu were supplemented 1.5 mmol/bottle (L-leucine HCl), and incubated after 0, 2, 4, 6, 8, 12, and 16 h to measure gas production (GP), nutrient degradability, fermentation parameters, bacterial composition, and amino acids metabolism. Results from both in vitro and in situ experiments confirmed that the rumen protection rate was greater (p < 0.01) in RP-Leu than in RU-Leu, whereas the latter was slow (p < 0.05) degraded within incubation 8 h. Free leucine from RP-Leu and RU-Leu reached a peak at incubation 6 h (p < 0.01). RU-Leu supplementation increased (p < 0.05) gas production, microbial crude protein, branched-chain AAs, propionate and branched-chain VFAs concentrations, and Shannon and Sobs index in comparison to the control and RP-Leu supplementation. RU-Leu and RP-Leu supplementation decreased (p < 0.05) the relative abundance of Bacteroidota, which Firmicutes increased (p < 0.05). Correlation analysis indicated that there are 5 bacteria at the genus level that may be positively correlated with MCP and propionate (p < 0.05). Based on the result, we found that RP-Leu was more stable than RU-Leu in rumen fluid, but RU-Leu also does not exhibit rapid degradation by ruminal microbes for a short time. The RU-Leu was more beneficial in terms of regulating rumen fermentation pattern, microbial crude protein synthesis, and branched-chain VFAs production than RP-Leu in vitro rumen conditions.
通讯机构：
[Yulong Yin; Jiashun Chen] A;Animal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan, 410128, China<&wdkj&>CAS Key Laboratory of Agro ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Changsha, Hunan, 410125, China<&wdkj&>Animal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan, 410128, China<&wdkj&>CAS Key Laboratory of Agro ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Changsha, Hunan, 410125, China
摘要：
This paper was to determine the effects of dietary Litsea cubeba essential oil (LEO) supplementation on growth performance, immune function, antioxidant level, intestinal morphology and microbial composition in weaned piglets. One hundred and ninety-two piglets (Duroc x [Large White x Landrace]) with 6.85 +/- 0.22 kg mean body weight weaned at 21 d of age were randomly assigned to 4 treatment groups with 8 replicates and were fed with a basal diet (CON) or CON diet containing 100 (LLEO), 200 (MLEO) and 400 (HLEO) mg/kg LEO. The results revealed that HLEO supplementation (P < 0.05) increased the average daily gain on d 28 compared with CON. MLEO and HLEO supplementation decreased (P < 0.05) feed conversion ratio. LEO-containing diets had a lower (P < 0.05) diarrhea rate. Supple-mentation with HLEO increased (P < 0.05) total antioxidant capacity (T-AOC) both in the serum and liver. Meanwhile, the supplementation of MLEO and HLEO resulted in higher (P < 0.05) glutathione peroxidase (GPx) activities both in serum and liver. Supplementation of HLEO increased (P < 0.05) serum immu-noglobulin A, immunoglobulin G and interleukin-10, whereas supplementation with MLEO and HLEO decreased (P < 0.05) tumor necrosis factor-a. Villus height in the duodenum or jejunum was increased (P < 0.05) in the HLEO group, and the villus height to crypt depth ratio in the jejunum was also improved (P < 0.05) in the MLEO group. The addition of LEO increased (P < 0.05) the richness and diversity of the microbial community in the cecum, which mainly increased the relative abundance of Oscillospir-aceae_UCG-005, Faecalibacterium, Blautia and Coprococcus. Piglets supplemented with HLEO increased (P < 0.05) the concentration of short chain fatty acids (SCFA), including acetic acid in the cecum and propionic acid in the colon. In conclusion, these findings indicated that LEO supplementation improved growth performance and intestinal health in weaned piglets. (c) 2023 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
关键词：
iron homeostasis;mitochondrial dysfunction;diseases
摘要：
Iron is a trace element necessary for cell growth, development, and cellular homeostasis, but insufficient or excessive level of iron is toxic. Intracellularly, sufficient amounts of iron are required for mitochondria (the center of iron utilization) to maintain their normal physiologic function. Iron deficiency impairs mitochondrial metabolism and respiratory activity, while mitochondrial iron overload promotes ROS production during mitochondrial electron transport, thus promoting potential disease development. This review provides an overview of iron homeostasis, mitochondrial iron metabolism, and how mitochondrial iron imbalances-induced mitochondrial dysfunction contribute to diseases.
摘要：
Branched-long-chain monomethyl fatty acids (BLCFA) are consumed daily in significant amounts by humans in all stages of life. BLCFA are absorbed and metabolized in human intestinal epithelial cells and are not only oxidized for energy. Thus far, BLCFA have been revealed to possess versatile beneficial bioactivities, including cytotoxicity to cancer cells, anti-inflammation, lipid-lowering, reducing the risk of metabolic disorders, maintaining normal β cell function and insulin sensitivity, regulation of development, and mitigating cerebral ischemia/reperfusion injury. However, compared to other well-studied dietary fatty acids like eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), BLCFA has received disproportionate attention despite their potential importance. Here we outlined the major food sources, estimated intake, absorption, and metabolism in human cells, and bioactive properties of BLCFA with a focus on the bioactive mechanisms to advocate for an increased commitment to BLCFA investigations. Humans were estimated to absorb 6-5000 mg of dietary BLCFA daily from fetus to adult. Notably, iso-15:0 inhibited the growth of prostate cancer, liver cancer and T-cell non-Hodgkin lymphomas in rodent models at the effective doses of 35-105 mg/kg/day, 70 mg/kg/day, and 70 mg/kg/day, respectively. Feeding formula prepared with 20% w/w BLCFA mixture to neonatal rats with enterocolitis mitigated the intestine inflammation. Iso-15:0 at doses of 10, 40, and 80 mg/kg relieved brain ischemia/reperfusion injury in rats. In the future, it is crucial to conduct research to establish the epidemiology of BLCFA intake and their impacts on health outcomes in humans as well as to fully uncover the underlying mechanisms for their bioactivities.
作者机构：
[Liao, Simeng; Wan, Dan; Wu, Xin; Li, Fengna; Yin, Yulong; Nie, Zhuyuan; Kong, Xiangfeng; Tang, Yulong; He, Liuqin] Laboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agro-ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;[Zhan, Kai; Tang, Yulong] Anhui Province Key Laboratory of Livestock and Poultry Product Safety Engineering, Hefei, 230001, China;[Tan, Bie; Ji, Chunxiao; Yin, Yulong; Nie, Zhuyuan] College of Animal Science and Technology, Hunan Agricultural University, Changsha, China;[Kuang, Guangwei] Hunan Provincial Institute of Animal Drug and Feed Supervision, Changsha, 410006, China
摘要：
Zearalenone (ZEA) exposure has carcinogenic effects on human and animal health by exhibiting intestinal, hepatic, and renal toxicity. At present, the underlying mechanisms on how ZEA induces apoptosis and damage to tissues still remain unclear. In this study, we aimed to identify genes that modulate the cellular response to ZEA using clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 screening, and further validate novel gene functions to elucidate molecular mechanisms underlying particular biological processes in vivo and in vitro. Two ZEA-resistant cell lines, designated Ov-KCNJ4 and Ov-KCNJ12, were yielded by CRISPR activation screening which had significant changes in ZEA resistance and growth rates. Results showed that ZEA could interact with the cell membrane proteins KCNJ4 and KCNJ12, inducing cell cycle arrest, disruption of DNA replication and base excision repair. Overexpression of KCNJ4 and KCNJ12 was involved in ZEA resistance by regulating cell cycle to neutralize toxicity, sustaining mitochondrial morphology and function via attenuating the damage from oxidative stress in the KCNJ4-mitoK(ATP) pathway. In vivo experiments showed that AAV-KCNJ4 delivery significantly improved ZEA-induced renal impairment and increased antioxidative enzyme activity by improving mitochondrial function. Our findings suggest that increasing potassium channel levels may be a putative therapeutic target for mycotoxin-induced damage.
摘要：
The purpose of this experiment was to use a single-factor design to investigate the effects of different supplementation levels of plant essential oil/palygorskite composite (EPO-Pal) on growth performance, blood parameters, and intestinal morphology of broiler chicken. A total of 960 one-day-old chicks were randomly assigned into 4 treatments with 6 replicates of 40 chicks each. The broilers were fed the basal diet supplemented with 0, 500, 750, and 1000 mg/kg EPO-Pal for 42 days, respectively. The results showed that the 500 or 750 mg/kg groups significantly increased the body weight, average daily feed intake and average daily gain of the broilers at 28 and 42 days (p < 0.05). Additionally, as compared to the control group, the 1000 mg/kg EPO-Pal group exhibited higher levels of immunoglobulins-A, immunoglobulins-G, immunoglobulins-M, and high-density lipoprotein cholesterol while having lower levels of tumour necrosis factor-alpha (p < 0.05). The content of interleukin-2 and the villus height-to-crypt depth ratio were significantly increased in the 750 mg/kg EPO-Pal group (p < 0.05). Moreover, the activity of total antioxidant capacity and superoxide dismutase were significantly enhanced in the 500 mg/kg EPO-Pal group (p < 0.05). The results are very promising, and EPO-Pal can be considered as an additive to promote the growth by enhancing immunity, antioxidant activity, and improving the intestinal morphology of broiler chickens.
通讯机构：
[Jun He; Mei Liu] A;Authors to whom correspondence should be addressed.<&wdkj&>College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Kunpeng Institute of Modern Agriculture at Foshan, Foshan 528226, China
摘要：
With the increasing popularity of genomic sequencing, breeders pay more attention to identifying the crucial molecular markers and quantitative trait loci for improving the body size and reproduction traits that could affect the production efficiency of pig-breeding enterprises. Nevertheless, for the Shaziling pig, a well-known indigenous breed in China, the relationship between phenotypes and their corresponding genetic architecture remains largely unknown. Herein, in the Shaziling population, a total of 190 samples were genotyped using the Geneseek Porcine 50K SNP Chip, obtaining 41857 SNPs for further analysis. For phenotypes, two body measurement traits and four reproduction traits in the first parity from the 190 Shaziling sows were measured and recorded, respectively. Subsequently, a genome-wide association study (GWAS) between the SNPs and the six phenotypes was performed. The correlation between body size and reproduction phenotypes was not statistically significant. A total of 31 SNPs were found to be associated with body length (BL), chest circumference (CC), number of healthy births (NHB), and number of stillborns (NSB). Gene annotation for those candidate SNPs identified 18 functional genes, such as GLP1R, NFYA, NANOG, COX7A2, BMPR1B, FOXP1, SLC29A1, CNTNAP4, and KIT, which exert important roles in skeletal morphogenesis, chondrogenesis, obesity, and embryonic and fetal development. These findings are helpful to better understand the genetic mechanism for body size and reproduction phenotypes, while the phenotype-associated SNPs could be used as the molecular markers for the pig breeding programs.
摘要：
As a member of the SNX family, the goat sorting nexin 29 (SNX29) is initially identified as a myogenesis gene. Therefore, this study aimed to examine the polymorphism in the SNX29 gene and its association with growth traits. In this study, we used an online platform to predict the structures of the SNX29 protein and used quantitative real-time PCR to detect potential copy number variation (CNV) in Shaanbei white cashmere (SBWC) goats (n = 541), Guizhou black (GB) goats (n = 48), and Nubian (NB) goats (n = 39). The results showed that goat SNX29 protein belonged to non-secretory protein. Then, five CNVs were detected, and their association with growth traits was analyzed. In SBWC goats, CNV1, CNV3, CNV4, and CNV5 were associated with chest width and body length (P < 0.05). Among them, the CNV1 individuals with gain and loss genotypes were superior to those individuals with a median genotype, but CNV4 and CNV5 of individuals with the median genotype were superior to those with the loss and gain genotypes. In addition, individuals with the gain genotype had superior growth traits in CNV3. In brief, this study suggests that the CNV of SNX29 can be used as a molecular marker in goat breeding.
作者机构：
[Shan, Yanke; Lu, Yu-Nan; Yi, Weijie; Wang, Bin; Li, Jiahao; Guo, Jiajing; Wang, Shouyu; Liu, Fei] Joint International Research Laboratory of Animal Health and Food Safety of Ministry of Education & Single Molecule Nanometry Laboratory (Sinmolab), Nanjing Agricultural University, Jiangsu, Nanjing;210095, China;[Li, Wenzhi] Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Shaanxi, Xi'an;710000, China;College of Animal Science and Technology, Hunan Agricultural University, Hunan, Changsha, China
通讯机构：
[Yulong Yin] C;[Fei Liu] J;College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan, China<&wdkj&>Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China<&wdkj&>Joint International Research Laboratory of Animal Health and Food Safety of Ministry of Education & Single Molecule Nanometry Laboratory (Sinmolab), Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China
摘要：
The purpose of this study was to evaluate the effects of limiting nutrients and the N: P ratios on the growth of phytoplankton. In this study, we used the nutrient enrichment bioassays (NEB), Multivariate analysis of variance, and regression analysis. The results showed that the affected change of phytoplankton assemblages growth was the concentrations of nitrogen and phosphorous. During the same period, the response time of phytoplankton to the input of external nutrients was similar. The growth rates of phytoplankton were not persistent. This is related to the differences in environmental conditions, dominant species composition, and phytoplankton growth strategies. Different dominant species also had different response mechanisms to nutrient addition. The growth effect of phytoplankton in the preliminary experiment in the laboratory was more significant than that in the field experiment. Water temperature and light were also important environmental factors affecting the growth of phytoplankton. Multivariate analysis of variance showed that phosphorus addition alone had a more significant stimulation on phytoplankton growth than that of nitrogen, especially for Synedra. But addition of both nitrogen and phosphorus had the most stimulating effect on Cyanophyta and Chlorophyta. The phytoplankton had the maximum growth in TN: TP = 19.87, phytoplankton growth was promoted with 0.9 & SIM; 13.59 mg/L TN and 0.02 & SIM; 0.684 mg/L TP. When the total phosphorus in summer was greater than 0.04 mg/L, the possibility of algal bloom and will be greater. The linear regression fitted chlorophyll a and phosphorus well, and the regression equation was as follows: Log(10)(Chl.a) = 2.154 + 0.5103 x Log(10)(TP). The binary regression equation of chlorophyll a and nitrogen and phosphorus was as follows: Chl.a = 33.84 + 1.769TN + 123.9TP.
