摘要：
A piezoelectric quartz crystal impedance technique has been applied for in situ monitoring of the whole process of the proteolytic enzyme-catalyzed hydrolyzation of casein and the effect of pesticide dimehypo (formula: C5H11NNa2O6S4) on the proteolytic enzyme activity in the midgut of the spider Pardosa pseudoannulata, based on its real-time reponses to the density-viscosity change of the tested solution due to the hydrolyzation occurring on the casein molecules. The results indicated that the hydrolyzation was significantly increased in the presence of low-dose dimehypo, where significantly inhibited in the presence of high-dose dimehypo, and a correlation was found precisely between the enzyme activity reflected by the final frequency shift after the hydrolyzation and the concentration of dimehypo, with a significant frequency response observed even at a 2.88 x 10-4 volume ratio of dimehypo to distilled water. The present work may have presented a novel method feasible for study on the mechanism of excitability of spiders under low-dose dimehypo pesticide and quick detection of proteolytic enzyme activity.
作者：
Ouyang, L. J.;He, W. H.;Huang, Z. C.;Zhao, L. Y.;Peng, S. H.;...
期刊：
JOURNAL OF TROPICAL FOREST SCIENCE,2012年24(2):198-208 ISSN：0128-1283
通讯作者：
Zeng, F. H.
作者机构：
[Huang, Z. C.; Sha, Y. E.; Zeng, F. H.; Ouyang, L. J.; He, W. H.; Zhao, L. Y.; Peng, S. H.] Zhanjiang Normal Univ, Coll Life Sci & Technol, Zhanjiang, Guangdong, Peoples R China.;[Sha, Y. E.; Lu, X. Y.; Ouyang, L. J.; He, W. H.; Zhao, L. Y.; Peng, S. H.] Hunan Agr Univ, Coll Biol Sci & Technol, Changsha, Hunan, Peoples R China.
通讯机构：
[Zeng, F. H.] Z;Zhanjiang Normal Univ, Coll Life Sci & Technol, Zhanjiang, Guangdong, Peoples R China.
摘要：
We developed an Agrobacterium tumefaciens-mediated transformation system for Eucalyptus urophylla using hypocotyl explants. Antibiotic concentrations, pre-culture times, pH of the inoculation medium and co-culture times were optimised. Pre-cultured hypocotyl explants were co-cultured with A. tumefaciens strain EHA105 harbouring the binary vector pPBR-2 containing the Rs-AFP2 gene, which encodes an antifungal protein, under the control of the prp1-1 promoter, for six days and were then transferred to selective callogenesis-inducing medium containing kanamycin and cefotaxime. Calluses developed shoots and were cultured in an elongation medium and finally multiplied. The integration of T-DNA into the genome of transgenic E. urophylla was confirmed by polymerase chain reaction (PCR). The reverse transcription (RT)-PCR results showed that Rs-AFP2 gene expression could be detected only after the transformed plants were inoculated with Phytophthora capsici 60 hours after inoculation. These results indicated that the prp1-1 promoter was inducible and Rs-AFP2 could enhance the resistance of E. urophylla to P. capsici. This protocol enabled effective transformation and regeneration of E. urophylla. Kami membangunkan sistem pengubahan berantarakan Agrobacterium tumefaciens bagi Eucalyptus urophylla menggunakan eksplan hipokotil. Kepekatan antibiotik, masa prakultur, pH medium inokulasi dan masa pengkulturan bersama dioptimumkan. Eksplan hipokotil dikultur bersama-sama dengan A. tumefaciens jenis EHA105 selama enam hari dan kemudiannya dipindahkan ke medium pengaruh pengkalusan terpilih yang mengandungi kanamisin dan sefotaksim. Baka ini menyimpan vektor perduaan pPBR-2 yang mengandungi gen Rs-AFP2 yang mengekod protein antikulat di bawah kawalan penggalak prp1-1. Kalus menghasilkan pucuk dan dikultur dalam medium pemanjangan dan kemudiannya digandakan. Integrasi T-DNA ke dalam genom E. urophylla yang transgenik disahkan oleh reaksi rantai polimerase (PCR). Keputusan trankripsi (RT)-PCR yang bertentangan menunjukkan yang gen Rs-AFP2 terperi boleh dikesan hanya selepas tumbuhan terubah telah diinokulasi dengan Phytophthora capsici (60 jam selepas inokulasi). Keputusan menunjukkan yang penggalak prp1-1 boleh diaruh dan Rs-AFP2 boleh meningkatkan kerintangan E. urophylla terhadap P. capsici. Protokol ini membolehkan pengubahan dan pertumbuhan semula E. urophylla yang berkesan.
摘要：
This paper includes the analysis of total content of Cd element of 102 samples in the surface soil of farmland around the slag field. We evaluated the contamination of Cd of each samples and the space distribution with the potential ecological risk index method based on ArcGIS soft. The results showed that: average of potential ecological risk index of Cd is 241.4, Moderate ecological risk area accounted for 2.7% of the study area, strong ecological risk area of the region accounted for 30.1% of the study area, 22.2% of the study area had the considerable ecological risk, and the rest 39% of the study area had the extremely strong ecological risk. Mineral resources provides important energy to national economy and our daily life, but with the development and utilization of mineral resources significantly increasing, the harm to the environment of heavy metals in smelting wastes gets more and more attention. Cd is a kind of hazardous element, if we intake cadmium-containing substances for a long while, we may Suffer from "Itai-Itai Disease" and other diseases or even die. There are lots of international and domestic researches about the ecological hazards of heavy metals on environment in the surrounding area of slag field in recent years. Pazi I has evaluated heavy metals in surface sediments of Candarli Gulf with the potential ecological risk index<sup>[1]</sup>. Zhao Xinna has done the research of the ecological pollution in the part of soil in Shanghai<sup>[2]</sup>. Wu Di has evaluated the potential ecological risk in the sediment of Wujiang River<sup>[3]</sup>. This paper takes the Cd element in the farmland near slag field as example, and evaluates the pollution caused by Cd element with the potential ecological risk index.
通讯机构：
[Zhang, Weiwei] S;Southwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Sichuan, Peoples R China.
会议名称：
International Conference on Contaminated Sites Remediation 2011 International Forum (RCST 2011)
会议时间：
OCT 25-27, 2011
会议地点：
Environm Sci Soc Chongqing, Chongqing, PEOPLES R CHINA
会议主办单位：
Environm Sci Soc Chongqing
会议论文集名称：
Advanced Materials Research
关键词：
Bioleaching;Pyritic mineral processing solid waste;Thiobacillus ferrooxidans;Thiobacillus thiooxidans
摘要：
The produced and extraction process of pyritic would generate a large amount of pyritic mineral processing solid waste. This kind of waste can lead to land occupation, and contaminate the local water, air and soil. Therefore, in this paper, Thiobacillus ferrooxidans and Thiobacillus thiooxidans are used to deal with the pyritic mineral processing solid waste. The particles, catalysts and bioleaching products between microorganism and pyritic mineral processing solid wast were studied. It is indicated that the microorganism growth and the heavy metals leaching efficiency involve biological and chemical interactions. The experimental results suggested that the T.t was best to bioleach the pyritic mineral processing solid waste, and the efficiency of T.f was worse than that of T.t. The leaching effect of mixed bacterias of T.t and T.f fell in between, the leaching rate would decrease when the pulp density increased. The micro-morphology of bacteria was observed by SEM detector, the single cellular dimension of bioleaching bactreia was about (1 similar to 2) mu mx (0.2 similar to 0.5) mu m.
关键词：
Soil erosion;Rare earth element (REE);Natural rainfall;Slope surface
摘要：
A field experiment using rare earth elements (REEs) as tracers was conducted to investigate soil erosion processes on slope surfaces during rainfall events. A plot of 10 m x 2 m x 0.16 m with a gradient of 20 degrees (36.4%) was established and the plot was divided into two layers and four segments. Various REE tracers were applied to the different layers and segments to determine sediment dynamics under natural rainfall. Results indicated that sheet erosion accounted for more than 90% of total erosion when the rainfall amount and density was not large enough to generate concentrated flows. Sediment source changed in different sections on the slope surface, and the primary sediment source area tended to move upslope as erosion progressed. In rill erosion, sediment discharge mainly originated from the toe-slope and moved upwards as erosion intensified. The results obtained from this study suggest that multi-REE tracer technique is valuable in understanding the erosion processes and determining sediment sources. (C) 2011 Elsevier Ltd. All rights reserved.
摘要：
A systematic strategy was developed for the proteomic analysis of wheat chloroplast protein complexes. First, comprehensive centrifugation methods were utilized for the exhaustive isolation of thylakoid, envelope, and stromal fractions. Second, 1% n-dodecyl-β-D-maltoside was selected from a series of detergents as the optimal detergent to dissolve protein complexes effectively from membranes. Then, blue native polyacrylamide gel electrophoresis (BN-PAGE) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) were improved to separate and analyze the protein complexes. By this systematic strategy, envelopes, thylakoids, and stromata were enriched effectively from chloroplasts in the same process, and more than 18 complexes were obtained simultaneously by BN-PAGE. Finally, thylakoid protein complexes were further analyzed by BN/SDS-PAGE, and nine complex bands and 40 protein spots were observed on BN-PAGE and SDS-PAGE respectively. Our results indicate that this new strategy can be used efficiently to analyze the proteome of chloroplast protein complexes and can be applied conveniently to the analysis of other subcellular protein complexes.
摘要：
To develop an efficient green extraction approach for recovery of bioactive compounds from natural plants, we examined the potential of pressurized liquid extraction (PLE) of ginger (Zingiber officinale Roscoe) with bioethanol/water as solvents. The advantages of PLE over other extraction approaches, in addition to reduced time/solvent cost, the extract of PLE showed a distinct constituent profile from that of Soxhlet extraction, with significantly improved recovery of diarylheptanoids, etc. Among the pure solvents tested for PLE, bioethanol yield the highest efficiency for recovering most constituents of gingerol-related compounds: while for a broad concentration spectrum of ethanol aqueous solutions, 70% ethanol gave the best performance in terms of yield of total extract, complete constituent profile and recovery of most gingerol-related components. PLE with 70% bioethanol operated at 1500 psi and 100 degrees C for 20 min (static extraction time: 5 min) is recommended as optimized extraction conditions, achieving 106.8%, 109.3% and 108.0% yield of [6]-, [8]- and [10]-gingerol relative to the yield of corresponding constituent obtained by 8 h Soxhlet extraction (absolute ethanol as extraction solvent). (C) 2011 Elsevier B.V. All rights reserved.
摘要：
A further chemical investigation of the plant Rubus corchorifolius L. f., collected in Hunan Province, afforded two new ent-kauranoids 6 and 7. Their structures were elucidated by various spectroscopic methods.
摘要：
Five flavones possessing one to four phenolic groups were fully phosphorylated efficiently and the obtained compounds showed excellent pancreatic cholesterol esterase (CEase) inhibitory activities with IC(50) in the nanomolar range, which were much more potent than their parent compounds. The inhibition mechanism and kinetic characterization studies indicate that they are irreversible competitive inhibitors.
