镉处理对大豆幼苗生长及激素含量的影响
作者：
黄运湘;廖柏寒;肖浪涛;刘素纯;王志坤
期刊：
环境科学 ,2006年27(7):1398-1401 ISSN：0250-3301
通讯作者：
Huang, Y.-X.
作者机构：
[黄运湘; 王志坤] 湖南农业大学资源环境学院;[廖柏寒] 中南林学院资源环境学院;[肖浪涛; 刘素纯] 湖南农业大学植物激素实验室
关键词：
大豆;镉污染;幼苗;植物激素
摘要：
采用室内培养试验，通过测定植株生理生化指标，研究了不同浓度Cd^2＋处理对大豆幼苗生长及激素含量的影响．结果表明：①不同浓度Cd^2＋处理对大豆幼苗地下部生长素（IAA）和赤霉素（GA3）的合成有抑制效应，对地上部生长素（IAA）和赤霉素（GA3）的合成具有低浓度下的刺激效应和高浓度下的抑制效应；对地下部、地上部玉米素（Z）和脱落酸（ABA）的合成均有刺激效应．②Cd^2＋处理84h对大豆幼苗根系活力、叶绿素a和叶绿素b的合成有刺激效应，对类胡萝卜素的合成无影响．③Cd^2＋处理对过氧化物酶（POD）活性和丙二醛（MDA）含量具有低浓度下的抑制效应和高浓度下的激活效应，且随着Cd^2＋浓度的增加激活效应逐渐加强．④短时间、低浓度Cd^2＋胁迫可刺激大豆幼苗的生理活性，以Cd^2＋处理浓度为0．50mg／L时，大豆幼苗根系活力最大，IAA和GA3含量最高，POD活性最低，植物生理活性最强．
语种：
中文
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Response of weeping willows to linear alkylbenzene sulfonate
作者：
Yu, Xiaozhang* ;Trapp, Stefan;Zhou, Puhua;Peng, Xiaoying;Cao, Xi
期刊：
Chemosphere ,2006年64(1):43-48 ISSN：0045-6535
通讯作者：
Yu, Xiaozhang
作者机构：
[Yu, Xiaozhang] Hunan Agr Univ, Dept Environm Sci, Changsha 410128, Hunan, Peoples R China.;Tech Univ Denmark, Inst Environm & Resources DTU, DK-2800 Lyngby, Denmark.;Hunan Agr Univ, Dept Biotechnol, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Yu, Xiaozhang] H;Hunan Agr Univ, Dept Environm Sci, Changsha 410128, Hunan, Peoples R China.
关键词：
LAS;linear alkylbenzene sulfonate;toxicity;transpiration;willows;enzyme activity;SLUDGE-AMENDED SOILS;RISK-ASSESSMENT;AGRICULTURAL SOIL;TERRESTRIAL ENVIRONMENT;SUPEROXIDE DISMUTASES;PLANTS
摘要：
Linear alkylbenzene sulfonate (LAS) is the most commonly used anionic surfactant in laundry detergents and cleaning agents. LAS compounds are found in surface waters and soils. The short-term acute toxicity of LAS to weeping willows (Salix babylonica L.) was investigated. Willow cuttings were grown in hydroponic solution spiked with LAS at 24.0 ± 1 °C for 192 h. The normalized relative transpiration of plants was used to determine toxicity. Severe reduction of the transpiration was only found for high doses of LAS ({greater than or slanted equal to}240 mg l-1). Chlorophyll contents in leaves of treated plants varied with the dose of LAS, but there was no significant linear correlation. The activities of the enzymes superoxide dismutases (SOD), catalase (CAT), and peroxidase (POD) were quantified at the end of experiments. At higher concentrations of LAS ({greater than or slanted equal to}240 mg l-1), the activities of SOD and CAT were decreased. The correlation between the dose of LAS and the POD activity in leaf cells was the highest of all enzyme assays (R2 = 0.5). EC50 values for a 50% inhibition of the transpiration of the trees were estimated to 374 mg l-1 (72 h) and 166 mg l-1 (192 h). Results from this experiment indicated that phytotoxic effects of LAS on willow trees are not expected for normal environmental conditions. © 2005 Elsevier Ltd. All rights reserved.
语种：
英文
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Effect of light on gene expression and shikonin formation in cultured Onosma paniculatum cells
作者：
Liu, Z;Qi, JL;Chen, L;Zhang, MS;Wang, XQ;...
期刊：
Plant Cell, Tissue and Organ Culture ,2006年84(1):39-46 ISSN：0167-6857
通讯作者：
Yang, YH
作者机构：
[Yang, YH] Nanjing Univ, Sch Life Sci, State Key Lab Pharmaceut Biotechnol, Plant Cell & Mol Biol Lab,Dept Biol Sci & Technol, Nanjing 210093, Peoples R China.;Hunan Agr Univ, Dept Biotechnol, Changsha 410128, Peoples R China.;Guizhou Univ, Sch Agron, Guiyang 550025, Peoples R China.
通讯机构：
[Yang, YH] N;Nanjing Univ, Sch Life Sci, State Key Lab Pharmaceut Biotechnol, Plant Cell & Mol Biol Lab,Dept Biol Sci & Technol, Nanjing 210093, Peoples R China.
关键词：
cell culture;shikonin;gene expression;light;Onosma paniculatum
摘要：
The cell culture in a production medium in the dark is capable of producing a large quantity of shikonin and its derivatives, which were completely inhibited when the cell cultures were irradiated with continuous white light and blue light and partially repressed under continuous red light. The expressions of PAL1, 4CL1, and CYP98A6 were induced under continuous red, blue and white light. Transcript levels of HMGR and LDI2 gradually were decreased in the dark and under continuous red light, while no transcriptional product of LDI2 was detected under blue and white light. C4H2 and LePGT1 genes were constantly expressed irrespective of different light conditions. Among the genes studied, the expression of LDI2 was critical for light-regulated shikonin formation. The inducible expression of PAL1, 4CL1, and CYP98A6 as well as the inhibitory transcription of LDI2 mediated by continuous red light irradiation were likely accounted for the reduced accumulation of shikonin.
语种：
英文
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一羟鬼笔毒肽（PHN）的制备及其毒理研究
作者：
魏宝阳;高国赋;陈作红
期刊：
湖南师范大学自然科学学报 ,2006年29(2):89-92 ISSN：2096-5281
通讯作者：
Wei, B.-Y.
作者机构：
[魏宝阳] 湖南师范大学生命科学学院,中国,长沙,410081;[高国赋; 魏宝阳] 湖南农业大学生物科学技术学院,中国,长沙,410128;[陈作红] 湖南师范大学
关键词：
灰花纹鹅膏菌;高效液相色谱;质谱;一羟鬼笔毒肽
摘要：
利用反相高效液相色谱技术对灰花纹鹅膏菌（Amanita fuliginea）的肽类毒素进行分离,并对保留时间为60.808min的成分进行了纯化,纯度达到99%.通过环肽检测、紫外光谱和质谱鉴定为一羟鬼笔毒肽（PHN）,其相对分子质量为772.36 Da.一羟鬼笔毒肽（PHN）能引起植物细胞坏死,对小白鼠的LD50为1 414μg·kg-1,能引起小白鼠肝脏充血,血液循环衰竭.
语种：
中文
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覆盖与间作对亚热带丘陵幼龄茶园土壤环境和生产的影响
作者：
宋同清;肖润林;彭晚霞;王久荣;李盛华;...
期刊：
农业工程学报 ,2006年22(7):60-64 ISSN：1002-6819
通讯作者：
Song, T.
作者机构：
[夏艳; 彭晚霞] 中国科学院亚热带农业生态研究所,长沙,410125;[夏艳; 彭晚霞] 湖南农业大学生物科学技术学院,长沙,410128;湘丰茶厂,长沙,410158;[王久荣; 肖润林; 宋同清] 中国科学院亚热带农业生态研究所;[李盛华] 湘丰茶厂
通讯机构：
Institute of Subtropical Agriculture, Chinese Academy of Sciences, China
关键词：
幼龄茶园;稻草覆盖;间作白三叶草;土壤环境;茶叶品质与产量
摘要：
以常规管理茶园（清耕茶园）为对照，通过连续4a大田对比试验，研究了稻草覆盖与间作白三叶草对亚热带丘陵幼龄茶园土壤环境及茶叶品质、产量的影响。研究结果表明：两种生物措施均降低了土壤容重，增加了土壤有机质含量，影响了土壤养分的供应状况，提高了土壤关键层次（0-20cm）和关键时期（4～6月）的水分含量，获得了不同观察时刻、不同深度土壤温度的多种调控效果，延缓和缩短了高温和干旱时间，促进了茶树生长，改善了茶叶品质，显著增加了茶叶产量，其名优绿茶酚氨比分别下降了0．68％和28．14％，产量提高了26．19％和32．65％，间作白三叶草的效果更好。
语种：
中文
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Mutation of the RESURRECTION1 Locus of Arabidopsis Reveals an Association of Cuticular Wax with Embryo Development
作者：
Chen, XB;Goodwin, SM;Liu, XL;Chen, XL;Bressan, RA;...
期刊：
PLANT PHYSIOLOGY ,2005年139(2):909-919 ISSN：0032-0889
通讯作者：
Jenks, MA
作者机构：
[Jenks, MA] Hunan Agr Univ, Crop Gene Engn Key Lab Human Province, Changsha 410128, Peoples R China.;Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47907 USA.
通讯机构：
[Jenks, MA] H;Hunan Agr Univ, Crop Gene Engn Key Lab Human Province, Changsha 410128, Peoples R China.
摘要：
Insertional mutagenesis of Arabidopsis (Arabidopsis thaliana) was used to identify a novel recessive mutant, designated resurrection1 (rst1), which possesses a dramatic alteration in its cuticular waxes and produces shrunken nonviable seeds due to arrested embryo development. The RST1 gene sequence associated with these phenotypes was verified by three independent, allelic, insertion mutants, designated rst1-1, rst1-2, and rst1-3, with inserts in the first exon, 12th intron, and fourth exon, respectively. These three rst1 allelic mutants have nearly identical alterations in their wax profiles and embryo development Compared to wild type, the wax on rst1 inflorescence stems is reduced nearly 60% in total amount, has a proportional reduction in aldehydes and aldehyde metabolites, and has a proportional increase in acids, primary alcohols, and esters. Compared to wild type, the C29 alkanes on rst1 are nearly 6-fold lower, and the C30 primary alcohols are 4-fold higher. These results indicate that rst1 causes shunting of most wax precursors away from alkane synthesis and into the primary-alcohol-producing branch of the pathway. In contrast to stems, the wax on rst1 mutant leaves increased roughly 43% in amount relative to the wild type, with the major increase occurring in the C31 and C33 alkanes. Unique among known wax mutants, approximately 70% of rst1 seeds are shrunken and nonviable, with these being randomly distributed within both inflorescence and silique. Viable seeds of rst1 are slightly larger than those of wild type, and although the viable rst1 seeds contain more total triacylglycerol-derived fatty acids, the proportions of these fatty acids are not significantly different from wild type. Shrunken seeds contain 34% of the fatty acids of wild-type seeds, with proportionally more palmitic, stearic, and oleic acids, and less of the longer and more desaturated homologs. Histological analysis of aborted rst1 seeds revealed that embryo development terminates at the approximate heart-shaped stage, whereas viable rst1 and wild-type embryos develop similarly. The RST1 gene encodes a predicted 1,841-amino acid novel protein with a molecular mass of 203.6 kD and a theoretical pI of 6.21. The RST1 transcript was found in all tissues examined including leaves, flowers, roots, stems, and siliques, but accumulation levels were not correlated with the degree to which different organs appeared affected by the mutation. The new RST1 gene reveals a novel genetic connection between lipid synthesis and embryo development; however, RST1's exact role is still quite unknown. The degree to which RST1 is associated with lipid signaling in development is an important focus of ongoing studies. © 2005 American Society of Plant Biologists.
语种：
英文
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CCoAOMT基因反义表达载体的构建及转化苎麻的研究
作者：
陈建荣;张学文;郭清泉;唐香山;杨友才
期刊：
湖南师范大学自然科学学报 ,2005年28(1):75-78 ISSN：2096-5281
通讯作者：
Guo, Q.-Q.(qingqg@yahoo.com)
作者机构：
[郭清泉; 陈建荣; 杨友才] Institution of Ramie, Hunan Agricultural University, Changsha 410128, China;[张学文; 唐香山] Departement of Biotechnology, Hunan Agricultural University, Changsha 410128, China;[陈建荣] College of Biology Resources and Environment, Jishou University, Jishou 416000, China
关键词：
苎麻(Boehmeria nivea);CCoAOMT基因;反义RNA;转化
摘要：
采用RT-PCR的方法,克隆烟草木质素合成关键酶咖啡酰甲基转移酶(CCoAOMT)基因并测序.构建CCoAOMT基因反义表达载体,通过农杆菌介导法对苎麻进行遗传转化,得到转基因植株.采用PCR方法对其进行分子检测.为利用基因工程技术,创造适合我国国情的环保型低木质素苎麻转基因新品种奠定了基础.
语种：
中文
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The preparation and catalytically active characterization of papain immobilized on magnetic composite microspheres
作者：
Lei, H;Wang, W;Chen, LL;Li, XC;Yi, B;...
期刊：
Enzyme and Microbial Technology ,2004年35(1):15-21 ISSN：0141-0229
通讯作者：
Deng, L
作者机构：
[Deng, L] Hunan Normal Univ, Dept Microbiol, Changsha 410081, Hunan, Peoples R China.;Hunan Agr Univ, Coll Nat Sci, Dept Biotechnol, Changsha 410128, Peoples R China.
通讯机构：
[Deng, L] H;Hunan Normal Univ, Dept Microbiol, Changsha 410081, Hunan, Peoples R China.
关键词：
Magnetic composite microspheres;Reactive acid chloride groups;The immobilized papain
摘要：
Magnetic composite microspheres containing carboxyl groups could be activated by thionylchloride. The carboxy microspheres produced reactive acid chloride groups which then react with the free amino groups of enzyme to give peptide bonds (-CO-NH-). Papain was mostly immobilized on the surface of the activated magnetic carriers by covalent binding. Freundlich isothermal binding model was established, the factors involved with the relative activity and the enzymic properties of the immobilized papain were studied in comparison with its soluble counterpart, for which casein was chosen as a substrate. The results showed that the pH, thermal and storage stabilities of the immobilized papain were higher than those of the soluble one. Apart from these, the Michaelis-Menten kinetic constants were evaluated for the immobilized papain and the soluble enzyme. In a word the immobilized papain exhibited better environmental adaptability and reusability than the soluble one. © 2004 Elsevier Inc. All rights reserved.
语种：
英文
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虎杖不同季节、不同组织部位白藜芦醇含量动态变化研究
作者：
曹庸;于华忠;李国章;张敏;廖书桥;...
期刊：
中国药学杂志 ,2004年39(5):337-339 ISSN：1001-2494
通讯作者：
Cao, Y.(caoyong2181@163.net)
作者机构：
[萧浪涛] Hunan Prov. Key Lab. Phytohormones, Hunan Agriculture University, Changsha 410128, China;[李国章; 张敏; 廖书桥; 于华忠] Key Lab. of Hunan Forest Product, Jishou University, Zhangjiajie 427000, China;[曹庸] Hunan Prov. Key Lab. Phytohormones, Hunan Agriculture University, Changsha 410128, China, Key Lab. of Hunan Forest Product, Jishou University, Zhangjiajie 427000, China
通讯机构：
[Cao, Y.] H;Hunan Prov. Key Lab. Phytohormones, Hunan Agriculture University, China
关键词：
高效液相色谱法;白藜芦醇;虎杖;组织部位
摘要：
目的观察虎杖不同季节、不同组织部位白藜芦醇含量的变化规律.方法通过高效液相色谱法对不同虎杖样品中白藜芦醇含量进行检测.结果虎杖的鲜根茎、鲜叶、鲜茎中,鲜根茎含量最高,为干根茎粉含量的3倍,且鲜根茎的芽中白藜芦醇含量极高.一年中虎杖白藜芦醇含量9月最高.结论虎杖白藜芦醇含量变化是有规律的.
语种：
中文
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Optimal sampling of a population to determine QTL location, variance, and allelic number
作者：
Wu, XL;Jannink, JL*
期刊：
Theoretical and Applied Genetics ,2004年108(7):1434-1442 ISSN：0040-5752
通讯作者：
Jannink, JL
作者机构：
[Jannink, JL] Iowa State Univ Sci & Technol, Dept Agron, Ames, IA 50011 USA.;Hunan Agr Univ, Ctr Life Sci Res, Hunan 410128, Peoples R China.
通讯机构：
[Jannink, JL] I;Iowa State Univ Sci & Technol, Dept Agron, Ames, IA 50011 USA.
摘要：
In a population intended for breeding and selection, questions of interest relative to a specific segregating QTL are the variance it generates in the population, and the number and effects of its alleles. One approach to address these questions is to extract several inbreds from the population and use them to generate multiple mapping families. Given random sampling of parents, sampling strategy may be an important factor determining the power of the analysis and its accuracy in estimating QTL variance and allelic number. We describe appropriate multiple-family QTL mapping methodology and apply it to simulated data sets to determine optimal sampling strategies in terms of family number versus family size. Genomes were simulated with seven chromosomes, on which 107 markers and six QTL were distributed. The total heritability was 0.60. Two to ten alleles were segregating at each QTL. Sampling strategies ranged from sampling two inbreds and generating a single family of 600 progeny to sampling 40 inbreds and generating 40 families of 15 progeny each. Strategies involving only one to five families were subject to variation due to the sampling of inbred parents. For QTL where more than two alleles were segregating, these strategies did not sample QTL alleles representative of the original population. Conversely, strategies involving 30 or more parents were subject to variation due to sampling of QTL genotypes within the small families obtained. Given these constraints, greatest QTL detection power was obtained for strategies involving five to ten mapping families. The most accurate estimation of the variance generated by the QTL, however, was obtained with strategies involving 20 or more families. Finally, strategies with an intermediate number of families best estimated the number of QTL alleles. We conclude that no overall optimal sampling strategy exists but that the strategy adopted must depend on the objective.
语种：
英文
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Amperometric immunosensor based on polypyrrole/poly(m-pheylenediamine) multilayer on glassy carbon electrode for cytokinin N6-(Δ2-isopentenyl) adenosine assay
作者：
Li, J;Xiao, LT;Zeng, GM;Huang, GH;Shen, GL* ;...
期刊：
Analytical Biochemistry ,2003年321(1):89-95 ISSN：0003-2697
通讯作者：
Shen, GL
作者机构：
[Shen, GL] Hunan Univ, Chem & Chem Engn Coll, Dept Environm Sci & Engn, State Key Lab Chem Biosensing & Chemometr, Changsha 410082, Peoples R China.;Hunan Agr Univ, Key Lab Phytohormones Hunan Province, Changsha 410028, Peoples R China.
通讯机构：
[Shen, GL] H;Hunan Univ, Chem & Chem Engn Coll, Dept Environm Sci & Engn, State Key Lab Chem Biosensing & Chemometr, Changsha 410082, Peoples R China.
关键词：
immunosensor;N-6-(Delta(2)-isopentenyl) adenosine (iPA);bienzyme catalysis;competitive immunoreaction
摘要：
A novel immunosensor based on a multilayer-coated glassy carbon electrode was designed to determine isopentenyl adenosine (iPA) in plants. The multilayer consists of polypyrrole and poly(m-phenylenediamine) with K 4Fe(CN)6 and horseradish peroxidase (HRP) entrapped during electropolymerization. The ferrocyanide doped in polypyrrole functions as the mediator. The glucose oxidase bound on the immunosensor by the competitive immunoreaction involving iPA catalyzed the oxidation of the added glucose with the formation of H2O2, which is in turn reduced in the presence of HRP entrapped in poly(m-phenylenediamine). The current of the oxidized production of ferrocyanide reduced at -50mV is inversely proportional to the concentration of iPA in the competitive immunoreaction. This immunosensor is able to be used about 40 times; after that its surface can be regenerated for a new immunosensor assembly by washing with 0.1M citrate-phosphate buffer (pH 4.6). The percentage of current response reduction (CR%) (y) is linearly related to the logarithm of the concentration of iPA (x) in the 5-300μg/ml range, with a regression equation of the form y=42.13x-27.79 and a correlation coefficient of 0.9861. Five hybrid rice grain samples were analyzed with results in satisfactory agreement to those obtained by high-performance liquid chromatography. © 2003 Elsevier Inc. All rights reserved.
语种：
英文
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A renewable amperometric immunosensor for phytohormone β-indole acetic acid assay
作者：
Li, J;Xiao, LT;Zeng, GM;Huang, GH;Shen, GL* ;...
期刊：
Analytica Chimica Acta ,2003年494(1-2):177-185 ISSN：0003-2670
通讯作者：
Shen, GL
作者机构：
[Shen, GL] Hunan Univ, Chem & Chem Engn Coll, State Key Lab Chemo Biosensing & Chemometr, Dept Environm Sci & Engn, Changsha 410082, Peoples R China.;Hunan Agr Univ, Key Lab Phytohormones Hunan Province, Changsha 410028, Peoples R China.
通讯机构：
[Shen, GL] H;Hunan Univ, Chem & Chem Engn Coll, State Key Lab Chemo Biosensing & Chemometr, Dept Environm Sci & Engn, Changsha 410082, Peoples R China.
关键词：
Amperometric immunosensor;Hapten-protein conjugation;Immunoassay;β-Indole acetic acid
摘要：
A rapid, convenient and accurate method using a sol-gel-alginate-carbon composite electrode (SACE) is described for the determination of β-indole acetic acid (IAA) in plant samples. The determination is based on an enzyme-linked competitive immunoreaction between free IAA and IAA labeled with HRP to bind on the anti-IAA IgG immobilized on the SACE surface. The response signal expressed as percentage current reduction (CR%, y) is linearly related to the logarithm of the concentration of IAA (x) in range of 5-500μgml -1 with a regression equation of the form y=37.80x-22.47 and correlation coefficient of 0.9922. The immunosensor was used to determine the IAA content in the hybrid rice grain samples taken in the growing period. © 2003 Published by Elsevier B.V.
语种：
英文
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Amperometric biosensor with HRP immobilized on a sandwiched nano-Au / polymerized m-phenylenediamine film and ferrocene mediator
作者：
Li, J;Xiao, LT;Liu, XM;Zeng, GM;Huang, GH;...
期刊：
Analytical and Bioanalytical Chemistry ,2003年376(6):902-907 ISSN：1618-2642
通讯作者：
Shen, GL
作者机构：
[Shen, GL] Hunan Univ, Dept Environm Sci & Engn, State Key Lab Chemobiosensing & Chemometr, Chem & Chem Engn Coll, Changsha 410082, Peoples R China.;Hunan Agr Univ, Key Lab Phytohormones Hunan Prov, Changsha 410028, Peoples R China.
通讯机构：
[Shen, GL] H;Hunan Univ, Dept Environm Sci & Engn, State Key Lab Chemobiosensing & Chemometr, Chem & Chem Engn Coll, Changsha 410082, Peoples R China.
关键词：
Enzyme biosensor;H2O2;m-Phenylenediamine polymer;Nano-Au particle
摘要：
An amperometric biosensor has been developed for the determination of H2O2 in plant samples. Horseradish peroxidase (HRP) is immobilized on a sandwiched nano-Au particle / m-phenylenediamine polymer film by glutaraldehyde cross-linking. The film is formulated on the carbon paste electrode (CPE) blended with ferrocene as an electron transfer mediator. On the low concentration range, the current response is related to the H 2O2 concentration linearly from 0 to 8×10 -6 M with a detection limit of 1.3×10-7 M. On a wider concentration range of 8×10-6 to 1.4×10 -4 M, the reciprocal of current response is linearly related to the reciprocal of H2O2 concentration. The apparent Michaelis-Menten constant (Km app) was calculated to be 0.0334 mM. The sensor has been tested by determining H2O2 concentration in plant leaf samples. © Springer-Verlag 2003.
语种：
英文
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A novel piezoelectric biosensor for the detection of phytohormone β-indole acetic acid
作者：
Li, J;Wu, ZY;Xiao, LT;Zeng, GM;Huang, GH;...
期刊：
Analytical Sciences ,2002年18(4):403-407 ISSN：0910-6340
通讯作者：
Shen, GL
作者机构：
[Shen, GL] Hunan Univ, Chem & Chem Engn Coll, Dept Environm Sci & Engn, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Peoples R China.;Hunan Agr Univ, Key Lab Phytohormones, Changsha 410082, Peoples R China.
通讯机构：
[Shen, GL] H;Hunan Univ, Chem & Chem Engn Coll, Dept Environm Sci & Engn, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Peoples R China.
摘要：
A novel piezoelectric immunosensor has been developed for the determination of β-indole acetic acid (IAA) in dilute solutions. The detection is based on competitive immunoreaction between a hapten (IAA) and an antigen (IAA-BSA, hapten-protein conjugation) bound to an anti-IAA antibody, immobilized on a quartz crystal microbalance (QCM). The frequency change (y) of the sensor caused by antigen is linearly related to the logarithm of the concentration of IAA (x) in the range of 0.5 ng/ml - 5 μg/ml with a regression equation of the form y = - 23x + 151 (r = 0.9937).
语种：
英文
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Analysis of Polygonatum odoratum and its substitutes by RAPD
作者：
刘塔斯;李钟;刘春林
期刊：
中国药学杂志 ,2002年37(10):734-736 ISSN：1001-2494
通讯作者：
Liu, T.-S.(LiuTasi@163.net)
作者机构：
[刘塔斯; 李钟] 湖南中医学院;[刘春林] 湖南农业大学
通讯机构：
[Liu, T.-S.] H;Human Coll. Traditional Chinese Med., Changsha 410007, China
关键词：
玉竹;黄精;遗传多样性;中药鉴定
摘要：
目的鉴别中药玉竹Polygonatumodoratum及其混淆品,探索药材鉴别的新方法,为玉竹品质评价提供分子依据.方法用随机扩增多态性DNA(RAPD)技术进行基因组DNA多态性分析,并构建聚类树型图.结果玉竹、黄精遗传多态性丰富,道地药材玉竹和其他产地的玉竹已产生遗传分化.结论 RAPD技术可应用于药材玉竹的鉴定,结合药效成分分析,可以成为玉竹品质评价的有力工具.
语种：
中文
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LAS和AE对水生植物损伤的显微和亚显微结构观察
作者：
刘红玉;廖柏寒;鲁双庆;周朴华;杨仁斌
期刊：
中国环境科学 ,2001年21(6):527-530 ISSN：1000-6923
通讯作者：
Liu, H.
作者机构：
[刘红玉; 廖柏寒; 杨仁斌] 湖南农业大学环境科学系;[鲁双庆] 长沙大学应用化学与环境保护系;[周朴华] 湖南农业大学生物技术系
通讯机构：
Department of Environmental Science, Hunan Agricultural University, China
关键词：
表面活性剂;水绵;水浮莲;污染;亚显微结构
摘要：
利用透射电镜研究了阴离子型表面活性剂LAS和非离子型表面活性剂AE对水生植物水绵、水浮莲的损伤作用.观察发现,在LAS处理液中,水绵细胞壁外层被溶解而消失,载色体的规则螺旋缠绕结构被打乱,集结成团.细胞膜与细胞壁部分分离.水浮莲细胞出现质壁分离,细胞膜部分解体,细胞质中有许多空腔,液泡增大,叶绿体变形;在AE处理液中,水绵细胞壁外层被溶解,细胞膜消失,载色体和造粉核解体,分散于整个由细胞壁内层构成的空腔中.水浮莲细胞膜部分解体,染色质浓缩,核膜逐渐解体,叶绿体和线粒体解体,液泡消失,被细胞质充填.可以推断:LAS和AE对水生植物损伤的机理不同,AE以溶解为主,LAS则除溶解外,所带电荷引起蛋白质构象改变也是主要因素之一.
语种：
中文
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拟南芥同源转换盒基因A21反义RNA基因重组体的构建及转化
作者：
张学文;罗泽民
期刊：
湖南师范大学自然科学学报 ,2001年24(1):62-65, 77 ISSN：2096-5281
通讯作者：
Zhang, X.-W.
作者机构：
[罗泽民; 张学文] Department of Biotechnology, Hunan Agricultural University, Changsha 410128, China
关键词：
拟南芥;反义RNA基因
摘要：
A21是拟南芥的一同源转换盒基因,在发育旺盛的组织中高度表达。采用反义RNA技术,将该基因cDNA 5'端片段410bp反向克隆到pTA7002后,构建成了可在植物中调控表达A21反义RNA基因片段的重组体。以根癌农杆菌浸渍法将重组体转入拟南芥Landsberg erecta生态型植株中,筛选到转A21反义RNA基因的拟南芥植株。反义基因可在甾类激素诱导下表达出A21的反义RNA。经初步诱导表达,变异性状表现为顶端生长抑制型。
语种：
中文
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浅海硬障碍物声波的反散射问题
作者：
陈明洪;段杰林;尹杰;刘金艳;李铁军;...
期刊：
中山大学学报(自然科学版) ,2000年39(4):35-38 ISSN：0529-6579
作者机构：
[陈明洪] 湖南农业大学生物科学技术学院;[段杰林; 刘金艳] 湖南农业大学动物科技学院;[尹杰; 李铁军] 中国科学院亚热带农业生态研究所;[方俊] 南农业大学生物科学技术学院
关键词：
反散射;硬障碍物;外向辐射条件;亥姆霍兹方程
摘要：
本研究旨在探讨霉变饲粮对育肥猪生长性能、脏器指数、血常规、血清生化指标、抗氧化指标以及肉品质的影响及添加1%精氨酸或2%谷氨酸对饲喂霉变饲粮造成的损伤的缓解作用。试验选用平均初始体重为(55.0±1.5) kg的"长×大"二元杂交育肥猪20头,随机分为4组,分别饲喂正常基础饲粮(对照组)、基础饲粮经过霉变处理后的霉变饲粮(霉变组)、添加1%精氨酸的霉变饲粮(精氨酸组)、添加2%谷氨酸的霉变饲粮(谷氨酸组),每组5个重复,每个重复1头猪。试验期60 d。结果表明:1)与对照组相比,霉变组育肥猪末重、平均日增重和平均日采食量显著降低(P<0.05),在饲粮中添加精氨酸或谷氨酸未能提高育肥猪生长性能。2)与对照组相比,霉变组肝脏指数显著升高,脾脏指数显著下降(P<0.05)。与霉变组相比,谷氨酸组肝脏指数显著降低,脾脏指数显著升高(P<0.05),缓解了霉变饲粮对肝脏、脾脏的损伤。3)与对照组相比,霉变组血清γ-谷氨酰胺转移酶活性显著升高(P<0.05),白蛋白和总蛋白含量显著降低(P<0.05)。与霉变组相比,精氨酸组血清谷丙转氨酶和乳酸脱氢酶活性显著降低(P<0.05)。4)与对照组相比,霉变组中平均红细胞体积显著下降(P<0.05),血小板压积显著升高(P<0.05)。与霉变组相比,精氨酸组中性粒细胞百分比和中间值细胞绝对值显著升高(P<0.05);谷氨酸组中间值细胞百分比、平均红细胞体积、平均血小板体积显著升高,血小板压积显著降低(P<0.05)。5)与对照组相比,霉变组血清超氧化物歧化酶活性显著下降(P<0.05)。与霉变组相比,谷氨酸组和精氨酸组血清谷胱甘肽过氧化物酶活性显著升高(P<0.05),不仅如此,谷氨酸组血清超氧化物歧化酶活性也显著升高(P<0.05)。6)与对照组相比,霉变组肌肉嫩度显著降低了20.22%(P<0.05)。精氨酸组肌肉嫩度较霉变组显著提高(P<0.05),且显著高于对照组(P<0.05)。综上所述,饲粮中添加1%精氨酸或2%谷氨酸能够对霉变饲粮造成的育肥猪脏器、抗氧化以及肉品质的损伤起到一定的缓解作用。
语种：
中文
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