摘要：
The rice stem borer (RSB, Chilo suppressalis) is a significant agricultural pest that mainly depends on chemical control. However, it has grown to varied degrees of pesticide resistance, which poses a severe threat to rice production and emphasizes the need for safer, more efficient alternative pest management strategies. Here, in vitro and in vivo experiments analyses reveal miR-1579 binds to the critical transcription factor Kruppel ho-mologue 1 (Kr-h1) and negatively regulates its expression. Overexpression of miR-1579 in larvae with signifi-cantly lower levels of Kr-h1 was associated with a decline in larval growth and survival. Furthermore, in female pupae, miR-1579 overexpression led to abnormalities in ovarian development, suggesting that targeting miR-1579 could be a potential management strategy against C. suppressalis. Therefore, we generated transgenic rice expressing miR-1579 and screened three lines that had a single copy of highly abundant mature miR-1579 transcripts. Expectedly, fed with transgenic miR-1579 rice lines were significantly lower survival rates in larvae and high levels of resistance to damage caused by C. suppressalis infestation. These findings suggest that miRNA-mediated RNAi could provide an effective and species-specific strategy for C. suppressalis control.
通讯机构：
[Bai, LY; Bai, LY ; Pan, L ] H;Hunan Agr Univ, Coll Plant Protect, Changsha, Peoples R China.;Hunan Agr Biotechnol Res Inst, Hunan Acad Agr Sci, Changsha, Peoples R China.
关键词：
Acetolactate synthase (ALS);Mesosulfuron-methyl;gene mutation;homology modelling and docking;Bromus japonicus
摘要：
Introduction: Bromus japonicus is one of the most notorious agricultural weeds in China. The long-term use of ALS-inhibiting herbicides has led to rapid evolution of herbicide resistance in B. japonicus. B. japonicus population (BJ-R) surviving mesosulfuron-methyl treatment was collected from wheatland. Here, we aimed to confirm the resistance mechanisms in this putative resistant population. Methods: The dose-reponse tests were used to test the resistance level of the B. japonicus to ALS-inhibiting herbicides. Pretreatment with P450 and GST inhibitors and GST activity assays were used to determine whether P450 or GST was involved in the resistance of the BJ-R population. Sanger sequencing was used to analyse the ALS mutation of the BJ-R population. RT-qPCR was used to confirm the the expression levels of the ALS gene in mesosulfuron-methyl -resistant (BJ-R) and-susceptible (BJ-S) B. japonicus. An in vitro ALS activity assay was used to determine the ALS activity of the BJ-R and BJ-S populations. Homology modelling and docking were used to determine the binding energy of the BJ-R and BJ-S populations with ALS-inhibiting herbicides. Results: B. japonicus population (BJ-R) was confirmed to be 454- and 2.7-fold resistant to the SU herbicides mesosulfuron-methyl and nicosulfuron, and 7.3-, 2.3-, 1.1- and 10.8-fold resistant to the IMI herbicide imazamox, the TP herbicide penoxsulam, the PTB herbicide pyribenzoxim and the SCT herbicide flucarbazone-sodium, respectively, compared with its susceptible counterpart (BJ-S). Neither a P450 inhibitor nor a GST inhibitor could reverse the level of resistance to mesosulfuron-methyl in BJ-R. In addition, no significant differences in GST activity were found between the BJ-R and BJ-S. ALS gene sequencing revealed a Pro-197-Thr mutation in BJ-R, and the gene expression had no significant differences between the BJ-R and BJ-S. The ALS activity of BJ-R was 106-fold more tolerant to mesosulfuron-methyl than that of BJ-S. Molecular docking showed that the binding energy of the ALS active site and mesosulfuron-methyl was changed from -6.67 to -4.57 kcal mol-1 due to the mutation at position 197. Discussion: These results suggested that the Pro-197-Thr mutation was the main reason for the high resistance level of BJ-R to mesosulfuron-methyl. Unlike previous reports of the cross-resistance pattern conferred by this mutation, we firstly documented that the Pro-197-Thr mutation confers broad cross-resistance spectrums to ALS-inhibiting herbicides in B. japonicus.
摘要：
Previous research has shown that the pathogenicity and appressorium development of Magnaporthe oryzae can be inhibited by the ATP synthase subunit beta (Atp2) present in the photosynthetic bacterium Rhodopseudomonas palustris. In the present study, transgenic plants overexpressing the ATP2 gene were generated via genetic transformation in the Zhonghua11 (ZH11) genetic background. We compared the blast resistance and immune response of ATP2-overexpressing lines and wild-type plants. The expression of the Atp2 protein and the physiology, biochemistry, and growth traits of the mutant plants were also examined. The results showed that, compared with the wild-type plant ZH11, transgenic rice plants heterologously expressing ATP2 had no significant defects in agronomic traits, but the disease lesions caused by the rice blast fungus were significantly reduced. When infected by the rice blast fungus, the transgenic rice plants exhibited stronger antioxidant enzyme activity and a greater ratio of chlorophyll a to chlorophyll b. Furthermore, the immune response was triggered stronger in transgenic rice, especially the increase in reactive oxygen species (ROS), was more strongly triggered in plants. In summary, the expression of ATP2 as an antifungal protein in rice could improve the ability of rice to resist rice blast.
摘要：
The root-knot nematode Meloidogyne graminicola secretes effectors into rice tissues to modulate host immunity. Here, we characterised MgCRT1, a calreticulin protein of M. graminicola, and identified its target in the plant. In situ hybridisation showed MgCRT1 mRNA accumulating in the subventral oesophageal gland in J2 nematodes. Immunolocalization indicated MgCRT1 localises in the giant cells during parasitism. Host-induced gene silencing of MgCRT1 reduced the infection ability of M. graminicola, while over-expressing MgCRT1 enhanced rice susceptibility to M. graminicola. A yeast two-hybrid approach identified the calmodulin-like protein OsCML31 as an interactor of MgCRT1. OsCML31 interacts with the high mobility group protein OsHMGB1 which is a conserved DNA binding protein. Knockout of OsCML31 or overexpression of OsHMGB1 in rice results in enhanced susceptibility to M. graminicola. In contrast, overexpression of OsCML31 or knockout of OsHMGB1 in rice decreases susceptibility to M. graminicola. The GST-pulldown and luciferase complementation imaging assay showed that MgCRT1 decreases the interaction of OsCML31 and OsHMGB1 in a competitive manner. In conclusion, when M. graminicola infects rice and secretes MgCRT1 into rice, MgCRT1 interacts with OsCML31 and decreases the association of OsCML31 with OsHMGB1, resulting in the release of OsHMGB1 to enhance rice susceptibility.
摘要：
The contamination of the plant phyllosphere with antibiotics and antibiotic resistance genes (ARGs), caused by application of antibiotics, is a significant environmental issue in agricultural management. Alternatively, biocontrol agents are environmentally friendly and have attracted a lot of interest. However, the influence of biocontrol agents on the phyllosphere resistome remains unknown. In this study, we applied biocontrol agents to control the wildfire disease in the Solanaceae crops and investigated their effects on the resistome and the pathogen in the phyllosphere by using metagenomics. A total of 250 ARGs were detected from 15 samples, which showed a variation in distribution across treatments of biocontrol agents (BA), BA with Mg(2+) (T1), BA with Mn(2+) (T2), and kasugamycin (T3) and nontreated (CK). The results showed that the abundance of ARGs under the treatment of BA-Mg(2+) was lower than that in the CK group. The abundance of cphA3 (carbapenem resistance), PME-1 (carbapenem resistance), tcr3 (tetracycline antibiotic resistance), and AAC (3)-VIIIa (aminoglycoside antibiotic resistance) in BA-Mg(2+) was significantly higher than that in BA-Mn(2+) (P < 0.05). The abundance of cphA3, PME_1, and tcr3 was significantly negatively related to the abundance of the phyllosphere pathogen Pseudomonas syringae (P < 0.05). We also found that the upstream and downstream regions of cphA3 were relatively conserved, in which rpl, rpm, and rps gene families were identified in most sequences (92%). The Ka/Ks of cphA3 was 0 in all observed sequences, indicating that under the action of purifying selection, nonsynonymous substitutions are often gradually eliminated in the population. Overall, this study clarifies the effect of biocontrol agents with Mg(2+) on the distribution of the phyllosphere resistome and provides evolutionary insights into the biocontrol process. IMPORTANCE: Our study applied metagenomics analysis to examine the impact of biocontrol agents (BAs) on the phyllosphere resistome and the pathogen. Irregular use of antibiotics has led to the escalating dissemination of antibiotic resistance genes (ARGs) in the environment. The majority of BA research has focused on the effect of monospecies on the plant disease control process, the role of the compound BA with nutrition elements in the phyllosphere disease, and the resistome is still unknown. We believe BAs are eco-friendly alternatives for antibiotics to combat the transfer of ARGs. Our results revealed that BA-Mg(2+) had a lower relative abundance of ARGs compared to the CK group, and the phyllosphere pathogen Pseudomonas syringae was negatively related to three specific ARGs, cphA3, PME-1, and tcr3. These three genes also present different Ka/Ks. We believe that the identification of the distribution and evolution modes of ARGs further elucidates the ecological role and facilitates the development of BAs, which will attract general interest in this field.
作者机构：
[Tangyu Yuan] Yantai Academy of Agricultural Science, No. 26 Gangcheng West Street, Fushan District, Yantai City 265500, China;College of Plant Protection, Hunan Agricultural University, Changsha 410128, China;[Ying Liu; Yingjie Zhao] State Key Laboratory for Quality and Safety of Agro-Products, Institute of Plant Virology, Ningbo University, Ningbo 315211, China;Authors to whom correspondence should be addressed.;These authors contributed equally to this work.
通讯机构：
[Wei Li; Jiaqian Liu] A;Authors to whom correspondence should be addressed.<&wdkj&>College of Plant Protection, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>State Key Laboratory for Quality and Safety of Agro-Products, Institute of Plant Virology, Ningbo University, Ningbo 315211, China
摘要：
Glycosylation, a dynamic modification prevalent in viruses and higher eukaryotes, is principally regulated by uridine diphosphate (UDP)-glycosyltransferases (UGTs) in plants. Although UGTs are involved in plant defense responses, their responses to most pathogens, especially plant viruses, remain unclear. Here, we aimed to identify UGTs in the whole genome of Nicotiana benthamiana (N. benthamiana) and to analyze their function in Chinese wheat mosaic virus (CWMV) infection. A total of 147 NbUGTs were identified in N. benthamiana. To conduct a phylogenetic analysis, the UGT protein sequences of N. benthamiana and Arabidopsis thaliana were aligned. The gene structure and conserved motifs of the UGTs were also analyzed. Additionally, the physicochemical properties and predictable subcellular localization were examined in detail. Analysis of cis-acting elements in the putative promoter revealed that NbUGTs were involved in temperature, defense, and hormone responses. The expression levels of 20 NbUGTs containing defense-related cis-acting elements were assessed in CWMV-infected N. benthamiana, revealing a significant upregulation of 8 NbUGTs. Subcellular localization analysis of three NbUGTs (NbUGT12, NbUGT16 and NbUGT17) revealed their predominant localization in the cytoplasm of N. benthamiana leaves, and NbUGT12 was also distributed in the chloroplasts. CWMV infection did not alter the subcellular localization of NbUGT12, NbUGT16, and NbUGT17. Transient overexpression of NbUGT12, NbUGT16, and NbUGT17 enhanced CWMV infection, whereas the knockdown of NbUGT12, NbUGT16 and NbUGT17 inhibited CWMV infection in N. benthamiana. These NbUGTs could serve as potential susceptibility genes to facilitate CWMV infection. Overall, the findings throw light on the evolution and function of NbUGTs.
作者机构：
College of Horticulture, Hunan Agricultural University , Changsha, China , 410128;Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding, Ministry of Education , Changsha, China , 410128;Key Laboratory for Vegetable Biology of Hunan Province , Changsha, China , 410128;[Jiahui Zhang; Yunsheng Wang] Hunan Provincial Key Laboratory for Biology and Control of Plant Disease and Insect Pests, Hunan Agricultural University , Changsha, China , 410128;[Qiuyun Wu; Shuxiang Mao; Huiping Huang; Juan Liu; Xuan Chen; Linghui Hou; Yuxiao Tian; Junwei Wang; Ke Huang] College of Horticulture, Hunan Agricultural University , Changsha, China , 410128<&wdkj&>Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding, Ministry of Education , Changsha, China , 410128<&wdkj&>Key Laboratory for Vegetable Biology of Hunan Province , Changsha, China , 410128
通讯机构：
[Junwei Wang; Ke Huang] C;[Yunsheng Wang] H;College of Horticulture, Hunan Agricultural University , Changsha, China , 410128<&wdkj&>Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding, Ministry of Education , Changsha, China , 410128<&wdkj&>Key Laboratory for Vegetable Biology of Hunan Province , Changsha, China , 410128<&wdkj&>Hunan Provincial Key Laboratory for Biology and Control of Plant Disease and Insect Pests, Hunan Agricultural University , Changsha, China , 410128
摘要：
Broccoli (Brassica oleracea var. italica Plenck) is an important vegetable crop, as it is rich in health-beneficial glucosinolates (GSLs). However, the genetic basis of the GSL diversity in Brassicaceae remains unclear. Here, we report a chromosome-level genome assembly of broccoli generated using PacBio HiFi reads and Hi-C technology. The final genome assembly is 613.79 Mb in size, with a contig N50 of 14.70 Mb. The GSL profile and content analysis of different Brassicas oleracea varieties, combined with a phylogenetic tree analysis, sequence alignment, and the construction of a three-dimensional model of the methylthioalkylmalate synthase 1 (MAM1) protein, which revealed that the gene copy number and amino acid sequence variation both contributed to the diversity of GSL biosynthesis in Brassica oleracea. The overexpression of BoMAM1 (BolI0108790) in broccoli resulted in high accumulation and a high ratio of C4-GSLs, demonstrating that BoMAM1 is the key enzyme in C4-GSLs biosynthesis. These results provide valuable insights for future genetic studies and nutritive component applications of Brassica crops.
通讯机构：
[Youzhi Li] H;Hunan Provincial Engineering & Technology Research Center for Biopesticide and Formulation Processing, Hunan Agricultural University, Changsha 410128, China<&wdkj&>National Research Center of Engineering & Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, China<&wdkj&>College of Plant Protection, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Author to whom correspondence should be addressed.
摘要：
Chilo suppressalis is one of the most damaging rice pests in China's rice-growing regions. Chemical pesticides are the primary method for pest control; the excessive use of insecticides has resulted in pesticide resistance. C. suppressalis is highly susceptible to cyproflanilide, a novel pesticide with high efficacy. However, the acute toxicity and detoxification mechanisms remain unclear. We carried out a bioassay experiment with C. suppressalis larvae and found that the LD10, LD30 and LD50 of cyproflanilide for 3rd instar larvae was 1.7 ng/per larvae, 6.62 ng/per larvae and 16.92 ng/per larvae, respectively. Moreover, our field trial results showed that cyproflanilide had a 91.24% control efficiency against C. suppressalis. We investigated the effect of cyproflanilide (LD30) treatment on the transcriptome profiles of C. suppressalis larvae and found that 483 genes were up-regulated and 305 genes were down-regulated in response to cyproflanilide exposure, with significantly higher CYP4G90 and CYP4AU10 expression in the treatment group. The RNA interference knockdown of CYP4G90 and CYP4AU10 increased mortality by 20% and 18%, respectively, compared to the control. Our results indicate that cyproflanilide has effective insecticidal toxicological activity, and that the CYP4G90 and CYP4AU10 genes are involved in detoxification metabolism. These findings provide an insight into the toxicological basis of cyproflanilide and the means to develop efficient resistance management tools for C. suppressalis.
摘要：
Rice blast is a globally devastating fungal disease that affects the production of rice (Oryza sativa), and the screening of excellent biocontrol strains is an important direction for the biological control of the fungus that causes rice blast disease (Magnaporthe oryzae). The objectives were to obtain strains that were highly antagonistic to rice blast, analyze the genetic information of the antagonistic bacterium YN-917, and explore the resources of its antagonistic gene cluster. The antagonistic bacteria were isolated and identified by the plate confrontation method, morphological observation, physiological and biochemical identification, and molecular biology methods. In addition, the strains were subjected to whole-genome sequencing, and their sequences were analyzed. Strain YN-917 was screened from healthy rice plants of the variety Xiangzaoxian 24, which is susceptible to rice blast, and it inhibited M. oryzae by 72.63% +/- 1.30%. Additionally, the strain had different degrees of inhibitory effects on various plant pathogenic fungi and was highly resistant to stress. The morphological observation, analysis of physiological and biochemical features, 16S rRNA homology analysis, and wholegenome sequencing analysis revealed that the strain YN-917 was Bacillus cereus (GenBank No.: PRJNA687285). The total length of its whole genome was 5326162 bp, and its average G + C content was 35.37%. It was composed of one circular chromosome and one endoplasmic plasmid. There were 5483 genes encoded on average. They included 105 tRNA genes, 42 self-replicating RNA (sRNA), 178 tandem repeat sequences, three prophages, and nine genomic islands. The prediction of antagonistic gene cluster demonstrated that the genome sequence of YN-917 had six secondary metabolic gene biosynthetic clusters, including those for bacteriocins, siderophores, non-ribosomal peptide synthetases (NRPs), and terpene. This study provides a theoretical basis to further explore microbial resources and their metabolic gene clusters for agricultural biological control.
通讯机构：
[Lianyang Bai; Lang Pan] A;Authors to whom correspondence should be addressed.<&wdkj&>College of Plant Protection, Hunan Agricultural University, No. 1, Nongda Road, Furong District, Changsha 410128, China
摘要：
Asia minor bluegrass (Polypogon fugax) is a common and problematic weed throughout China. P. fugax that is often controlled by acetyl-CoA carboxylase (ACCase) inhibitors in canola fields. Herein, we confirmed a P. fugax population (R) showing resistance to all ACCase inhibitors tested with resistance indexes ranging from 5.4–18.4. We further investigated the resistance mechanisms of this R population. Molecular analyses revealed that an amino acid mutation (Asp-2078-Gly) was present in the R population by comparing ACCase gene sequences of the sensitive population (S). In addition, differences in susceptibility between the R and S population were unlikely to be related to herbicide metabolism. Furthermore, a new derived cleaved amplified polymorphic sequence (dCAPS) method was developed for detecting the Asp-2078-Gly mutation in P. fugax efficiently. We found that 93.75% of plants in the R population carried the Asp-2078-Gly mutation, and all the herbicide-resistant phenotype of this R population is inseparable from this mutation. This is the first report of cross resistance to ACCase inhibitors conferred by the Asp-2078-Gly target-site mutation in P. fugax. The research suggested the urgent need to improve the diversity of weed management practices to prevent the widespread evolution of herbicide resistance in P. fugax in China.
通讯机构：
[Lin Qiu; Lin Qiu Lin Qiu Lin Qiu] H;Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha, China
作者机构：
[Wang, Zihao; Li, Xiaogang; Li, Chaonan; Wang, Ya] Hunan Agr Univ, Coll Plant Protect, Engn & Technol Res Ctr Bio Pesticide & Formulating, Changsha 410128, Peoples R China.
通讯机构：
[Xiaogang Li] E;Engineering & Technology Research Center for Bio Pesticide and Formulating Processing, College of Plant Protection, Hunan Agricultural University, Changsha410128, China
摘要：
Although paraquat is a widely used herbicide, it is toxic to humans if ingested or absorbed through an open wound. To improve the safety of paraquat, a new formulation of paraquat based on photoresponsive polymers was exploited. Photoresponsive β-cyclodextrin polymer microspheres (AZO-CD) were synthesized via a host-guest interaction between β-cyclodextrin and azobenzene. AZO-CD were characterized by Fourier transform infrared spectrometry, circular dichroism, ultraviolet (UV) spectrophotometry, and thermogravimetric analysis, and their photoresponsiveness was also evaluated. AZO-CD were used to load paraquat, which yielded photoresponsive paraquat-loaded microspheres. For the paraquat-loaded microspheres, irradiation with UV light or sunlight induced the isomerization of azobenzene into the cis form. Then, the cis-azobenzene was liberated from the cavities of the β-cyclodextrin. The paraquat-loaded microspheres released paraquat continuously over time. Furthermore, under UV light, the herbicidal capacity of the paraquat-loaded microspheres against barnyard grass was comparable to that of free paraquat at the same dose. Our findings show that loading paraquat into AZO-CD provides a safe and environmentally friendly herbicide formulation.
期刊：
Zoological Journal of the Linnean Society,2023年198(2):476-493 ISSN：0024-4082
通讯作者：
Xing Wang<&wdkj&>Guo-Hua Huang
作者机构：
[Liao, Cheng-Qing; Chen, Qi; Huang, Guo-Hua; Chen, Lu; Wang, Xing] Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.;[Liao, Cheng-Qing; Chen, Qi; Huang, Guo-Hua; Chen, Lu] Hunan Agr Univ, Hunan Prov Key Lab Biol & Control Plant Dis & Inse, Nongda Rd 1, Changsha 410128, Hunan, Peoples R China.;[Yagi, Sadahisa; Hirowatari, Toshiya] Kyushu Univ, Fac Agr, Entomol Lab, 744 Motooka,Nishi Ku, Fukuoka 8190395, Japan.;[Wang, Xing] Qiongtai Normal Univ, Coll Sci, Haikou 571100, Hainan, Peoples R China.;[Wang, Xing] South China Agr Univ, Dept Entomol, Guangzhou 510640, Guangdong, Peoples R China.
通讯机构：
[Xing Wang; Guo-Hua Huang] C;College of Plant Protection, Hunan Agricultural University , Changsha, Hunan 410128 , China<&wdkj&>Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University , Nongda Road 1, Furong District, Changsha, Hunan 410128 , China<&wdkj&>College of Plant Protection, Hunan Agricultural University , Changsha, Hunan 410128 , China<&wdkj&>College of Science, Qiongtai Normal University , Haikou, Hainan 571100 , China
摘要：
As one of the largest single-radiation sources of phytophagous insects, the higher level phylogeny of lepidopteran lineages has been debated for decades, especially the early divergences of nonditrysia. Here, we use 74 mitochondrial genomes representing seven nonditrysian superfamilies, including the newly sequenced mitochondrial genomes of eight families and 30 species. Four datasets were constructed and used for a phylogenetic analysis based on maximum likelihood (ML) and Bayesian inference (BI) methods. The results reveal that (1) nonditrysian lineages presented typically nested relationships with the family Micropterigidae as the sister-group to the remaining Lepidoptera, (2) monophyly of major clades in Lepidoptera, including Glossata, Heteroneura, Eulepidoptera and Ditrysia, was strongly supported, and (3) Neopseustoidea formed the sister of the remaining Glossata. Divergence-time analysis estimated that Lepidoptera originated c. 222 Mya during the Middle Triassic and that most superfamilies/families of nonditrysia originated from the Early Cretaceous to the Early Palaeogene, which largely coincided with the radiation of angiosperms. Additionally, three novel gene rearrangements in the gene cluster 'MIQ' and 'TP' were found in nonditrysian groups. Further mitochondrial genome data will help us to better understand the evolution of early nonditrysian lineages.
通讯机构：
[Huan Yu; Gong Chen; Huan Yu Huan Yu Huan Yu; Gong Chen Gong Chen Gong Chen] H;Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, Hunan, People's Republic of China<&wdkj&>College of Plant Protection, Hunan Agricultural University, Changsha, Hunan, People's Republic of China
关键词：
Surface ion imprinting;support;heavy metal ions;adsorption
摘要：
Surface ion-imprinting technology (SIIT) is a novel and effective alternative to conventional methods of preparing ion-imprinted polymers. SIIT involves the coating of support materials with a polymeric layer that selectively binds with imprinted metal ions, particularly heavy metal ions. Given its specificity, predictability, and stability, SIIT has been quickly adopted in ion detection, separation, enrichment, and sensing. Here, building on the latest advances in this emerging new technology, we carried out a systematic review to summarize the 1) principles and basic components of surface ion-imprinted polymers (SIIPs); 2) preparations, properties, advantages, and disadvantages of three types of SIIP supporting materials, including inorganic (minerals, carbonaceous materials, metal oxides), organic, and composite (organic/organic, organic/inorganic, inorganic/inorganic) supports; 3) current applications of SIIT, and 4) future challenges and opportunities related to SIIT. Finally, perspectives and future research are discussed to address the pressing need for the development of SIIT for heavy metal ion treatment.
