低氮密植对机插双季稻产量形成和氮肥利用率的影响
作者：
杨志长;沈涛;罗卓;彭芝;胡宇倩;...
期刊：
作物杂志 ,2020年(02):71-81 ISSN：1001-7283
作者机构：
湖南农业大学资源环境学院,410128,湖南长沙;[胡宇倩; 罗卓; 宋海星; 资涛; 彭芝; 沈涛; 杨志长; 熊廷浩] 湖南农业大学
关键词：
机插双季稻;低氮密植;产量形成;氮肥利用率
摘要：
在大田试验条件下,采用裂区设计,以株两优819和中嘉早17为早稻材料,泰优390和湘晚籼13为晚稻材料,设置不施氮常密(CK0)、不施氮高密(CK1)、常氮常密(D0N0)、低氮高密(D1N1)4种氮肥密度组合,研究低氮密植对机插早、晚稻产量形成和氮肥利用率的影响.结果 表明,与D0N0相比,D1N1更有利于构建水稻优良群体结构,提高群体分蘖数和主要生育期干物质含量,且叶面积指数、光合势和群体生长率等群体指标均具有一定的优势;同时,D1N1协调穗数与粒数的矛盾,提高群体总颖花量,维持结实率和千粒重,保持产量稳定;D1N1还可提高氮素偏生产力、氮素农学利用率和氮素吸收利用率,但对氮收获指数影响较小.低氮密植对水稻经济效益的影响因品种、稻季和地点的不同而存在差异,有一定的减收风险.综合考虑,低氮密植可兼顾水稻产量的稳定和氮肥利用率的提高,且氮肥投入的减少有利于环保.
语种：
中文
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Balance between nitrogen use efficiency and cadmium tolerance in Brassica napus and Arabidopsis thaliana.
作者：
Liao, Qiong;Jian, Shao-Fen;Song, Hai-Xing;Guan, Chun-Yun;Lepo, Joe Eugene;...
期刊：
Plant Science ,2019年284:57-66 ISSN：0168-9452
通讯作者：
Zhang, Zhen-Hua
作者机构：
[Song, Hai-Xing; Zhang, Zhen-Hua; Liao, Qiong; Jian, Shao-Fen] Hunan Agr Univ, Coll Resources & Environm Sci, Southern Reg Collaborat Innovat Ctr Grain & Oil C, Changsha, Hunan, Peoples R China.;[Song, Hai-Xing; Zhang, Zhen-Hua] Hunan Agr Univ, Natl Engn Lab High Efficiency Utilizat Soil & Fer, Changsha, Hunan, Peoples R China.;[Guan, Chun-Yun] Natl Ctr Oilseed Crops Improvement, Hunan Branch, Changsha, Hunan, Peoples R China.;[Lepo, Joe Eugene] Univ West Florida, Ctr Environm Diagnost & Bioremediat, Pensacola, FL 32514 USA.;[Ismail, Abdelbagi M.] Int Rice Res Inst, DAPO 7777, Manila, Philippines.
通讯机构：
[Zhang, Zhen-Hua] H;Hunan Agr Univ, Coll Resources & Environm Sci, Southern Reg Collaborat Innovat Ctr Grain & Oil C, Changsha, Hunan, Peoples R China.;Hunan Agr Univ, Natl Engn Lab High Efficiency Utilizat Soil & Fer, Changsha, Hunan, Peoples R China.
关键词：
Heavy metal tolerance;Nitrate assimilation;Nitrogen use efficiency;Proton pumps;Vacuolar sequestration capacity (VSC)
摘要：
The transmembrane transport of NO3(-) and Cd(2+) into plant cell vacuoles relies on the energy from their tonoplast proton pumps, V-ATPase and V-PPase. If the activity of these pumps is reduced, it results in less NO3(-) and Cd(2+) being transported into the vacuoles, which contributes to better nitrogen use efficiency (NUE) and lower Cd(2+) tolerance in plants. The physiological mechanisms that regulate the balance between NUE and Cd(2+) tolerance remain unknown. In our study, two Brassica napus genotypes with differential NUEs, xiangyou 15 and 814, and Atclca-2 mutant and AtCAX4 over-expression line (AtCAX4-OE) of Arabidopsis thaliana, were used to investigate Cd(2+) stress responses. We found that the Brassica napus genotype, with higher NUE, was more sensitive to Cd(2+) stress. The AtCAX4-OE mutant, with higher Cd(2+) vacuolar sequestration capacity (VSC), limited NO3(-) sequestration into root vacuoles and promoted NUE. Atclca-2 mutants, with decreased NO3(-) VSC, enhanced Cd(2+) sequestration into root vacuoles and conferred greater Cd(2+) tolerance than the WT. This may be due to the competition between Cd(2+) andNO3(-) in the vacuoles for the energy provided by V-ATPase and V-PPase. Regulating the balance between Cd(2+) and NO3(-) vacuolar accumulation by inhibiting the activity of CLCa transporter and increasing the activity of CAX4 transporter will simultaneously enhance both the NUE and Cd(2+) tolerance of Brassica napus, essential for improving its Cd(2+) phytoremediation potential.
语种：
英文
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湖南省油菜施肥现状与减肥空间分析
作者：
胡宇倩;张秋平;鲁艳红;李旭霞;杨志长;...
期刊：
湖南农业科学 ,2019年(04):31-36 ISSN：1006-060X
作者机构：
湖南农业大学资源环境学院,土壤肥料资源高效利用国家工程实验室,农田污染控制与农业资源利用湖南省重点实验室,植物营养湖南省普通高等学校重点实验室,湖南长沙410128;[张秋平; 胡宇倩; 宋海星; 鲁艳红; 杨志长; 李旭霞] 植物营养湖南省普通高等学校
关键词：
油菜;施肥;减肥空间;湖南
摘要：
通过农户抽样调查的方法,分析研究了湖南省2016-2017年度油菜施肥情况.结果 表明:所调查的农户100％施用了化肥,而仅有11.2％农户施用了有机肥.N∶ P205∶K20的比例为1∶0.42∶0.51,而高化肥氮投入量前50％的样本中,化肥平均投入量的N∶ P2O5∶K2O比例为1∶0.36∶0.36.结合湖南省推荐施肥量分析表明,30％、50％的农户分别有约30％、20％的节氮、磷空间,30％的农户有18.4％的节钾空间,50％的农户有5.7％的节钾空间.油菜施肥次数以2次所占比例最高,基本施肥方式为基肥+1次追肥、苗床肥+基肥,45.6％的氮肥作基肥施用.由此提出湖南省油菜种植的改进措施主要有:提倡有机肥替减化肥;营养元素协同增效减肥;应用新型肥料与改进施肥方法.
语种：
中文
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A Biochar-Based Route for Environmentally Friendly Controlled Release of Nitrogen: Urea-Loaded Biochar and Bentonite Composite
作者：
Liu, Xiangrong;Liao, Jiayuan;Song, Haixing;Yang, Yong;Guan, Chunyun;...
期刊：
Scientific Reports ,2019年9(1) ISSN：2045-2322
通讯作者：
Zhang, Zhenhua
作者机构：
[Guan, Chunyun; Song, Haixing; Liu, Xiangrong; Zhang, Zhenhua; Liao, Jiayuan; Yang, Yong] Hunan Agr Univ, Coll Resources & Environm Sci, Southern Reg Collaborat Innovat Ctr Grain & Oil C, Changsha 410128, Hunan, Peoples R China.;[Liu, Xiangrong] Hunan Qidong Country Tobacco Co, Hengyang 421600, Peoples R China.;[Song, Haixing; Liu, Xiangrong; Zhang, Zhenhua; Liao, Jiayuan; Yang, Yong] Natl Engn Lab Soil & Fertilizer Resources Efficie, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Zhang, Zhenhua] H;[Zhang, Zhenhua] N;Hunan Agr Univ, Coll Resources & Environm Sci, Southern Reg Collaborat Innovat Ctr Grain & Oil C, Changsha 410128, Hunan, Peoples R China.;Natl Engn Lab Soil & Fertilizer Resources Efficie, Changsha 410128, Hunan, Peoples R China.
摘要：
Biochar-based fertilizers have attracted increased attention, because biochar can improve the soil fertility, promote plant growth and crop yield. However, biochar-based controlled release nitrogen fertilizers (BCRNFs) still face problems because of the high cost, inefficient production technology, instability of nitrides, and the challenge associated with the controlled release of nutrients. In this study, we hydrothermally synthesised novel BCRNFs using urea-loaded biochar, bentonite and polyvinyl alcohol for controlled release of nutrients. Scanning electron microscopy and gas adsorption were conducted to identify the urea-loading and storage of bentonite in the inner pores of the biochar particles. X-ray diffraction, Fourier transform infrared spectroscopic and X-ray photoelectron spectroscopic studies demonstrated that strengthening the interactions among biochar, urea, and bentonite, helps control the moisture diffusion and penetration of bentonite, thereby leading to nutrient retention. The BCRNF showed significantly improved nutrient release characteristic compared with that of a mixture of biochar and urea. This urea-bentonite composite loaded with urea provides control over the release of nutrients stored in the biochar. BCRNF, especially those produced hydrothermally, can have potential applications in sustainable food security and green agriculture.
语种：
英文
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甘蓝型油菜钙离子转运蛋白CAX家族基因生物信息学及其对镉胁迫响应表达分析
作者：
廖琼;周婷;肖燕;唐天骄;宋海星;...
期刊：
植物生理与分子生物学学报 ,2019年55(5):596-608 ISSN：1671-3877
通讯作者：
Zhang, Z.-H.
作者机构：
湖南农业大学资源环境学院, 长沙, 410128;南方粮油作物协同创新中心, 南方粮油作物协同创新中心, 长沙, 410128;国家油料作物改良中心湖南分中心, 国家油料作物改良中心湖南分中心, 长沙, 410128;[华营鹏; 肖燕; 唐天骄; 周婷; 张振华; 宋海星; 廖琼] 湖南农业大学;[官春云] 国家油料作物改良中心
通讯机构：
College of Resources and Environmental Sciences, Hunan Agricultural University, Changsha, China
关键词：
甘蓝型油菜;镉胁迫;生物信息学
摘要：
CAXs (Cation/H~+exchanger antiporter)是一类重要的阳离子跨膜转运蛋白,在调控植物Ca~(2+)平衡,参与转运金属离子Cd~(2+)和Mn~(2+)中发挥了重要作用,但在甘蓝型油菜中尚缺乏系统研究。本文利用生物信息学的方法对甘蓝型油菜CAX家族成员的基因拷贝数、系统进化、进化选择压力、分子特征、保守基序、染色体定位、基因结构及其启动子区域所能结合的顺式作用元件进行了预测和分析;同时采用荧光定量PCR分析了甘蓝型油菜BnaCAXs的组织表达模式及其对Cd~(2+)胁迫的响应。结果表明,甘蓝型油菜CAX家族共包含17个成员,系统进化分析结果表明BnaCAXs与拟南芥进化相似,并且明显地分为5个亚家族。BnaCAXs家族所有基因成员的Ka/Ks值均小于0.3,受到强烈的纯化选择作用。大部分BnaCAXs均属于稳定的疏水蛋白,并包含8~11个跨膜结构域。基因结构差异明显,内含子数目从6~11不等,并且Dof、MYB以及W-BOX是其启动子上丰度较大的顺式作用元件,可能参与到BnaCAX抗逆过程的调控。荧光定量PCR结果表明,甘蓝型油菜CAX基因主要在地下部表达; BnaC4.CAX1-2和BnaC3.CAX2-2是该家族的核心基因,并受到Cd~(2+)胁迫的显著诱导。
语种：
中文
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Low concentration of exogenous ethanol promoted biomass and nutrient accumulation in oilseed rape (Brassica napus L.)
作者：
Wu, Zhimin;Yang, Lan;Jiang, Lihong;Zhang, Zhenhua;Song, Haixing;...
期刊：
PLANT SIGNALING & BEHAVIOR ,2019年14(12):1681114 ISSN：1559-2316
通讯作者：
Han, Yongliang
作者机构：
[Song, Haixing; Zhang, Zhenhua; Rong, Xiangmin; Yang, Lan; Han, Yongliang; Wu, Zhimin] Hunan Agr Univ, Coll Resources & Environm, Changsha 410128, Hunan, Peoples R China.;[Jiang, Lihong] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China.
通讯机构：
[Han, Yongliang] H;Hunan Agr Univ, Coll Resources & Environm, Changsha 410128, Hunan, Peoples R China.
关键词：
Ethanol;nutrient accumulation;oilseed rape
摘要：
With hydroponics culture, we monitored the response of the growth and nutrient accumulation of oilseed rape (Brassica napus L.) to five ethanol concentrations: 0 mL•L−1 (control), 0.0125 mL•L−1, 0.025 mL•L−1, 0.05 mL•L−1, and 0.25 mL•L−1, respectively. The results showed that a high concentration of exogenous ethanol (0.25 mL•L−1) significantly inhibited oilseed rape growth by 52.28%. However, the biomass of oilseed rape with a low concentration of exogenous ethanol (0.0125–0.05 mL•L−1) manipulation was raised by 16.62–44.08%. A similar result was found on the total nitrogen, phosphorus, and potassium of the oilseed rape. Results of micro-element determination showed that iron and zinc accumulation in oilseed rape was unchanged, while manganese and copper accumulation was increased first and then decreased with increasing ethanol concentration. This study provided a possibility for improving plant growth with low concentration ethanol application in oilseed rape planting. © 2019, © 2019 Taylor & Francis Group, LLC.
语种：
英文
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A multiomics approach reveals the pivotal role of subcellular reallocation in determining rapeseed resistance to cadmium toxicity
作者：
Zhang, Zhen-Hua;Zhou, Ting;Tang, Tian-Jiao;Song, Hai-Xing;Guan, Chun-Yun;...
期刊：
JOURNAL OF EXPERIMENTAL BOTANY ,2019年70(19):5437-5455 ISSN：0022-0957
通讯作者：
Hua, Ying-Peng
作者机构：
[Zhang, Zhen-Hua; Zhou, Ting; Tang, Tian-Jiao; Song, Hai-Xing; Hua, Ying-Peng] Hunan Agr Univ, Coll Resources & Environm Sci, Southern Reg Collaborat Innovat Ctr Grain & Oil C, Changsha, Hunan, Peoples R China;[Huang, Jin-Yong; Hua, Ying-Peng] Zhengzhou Univ, Sch Agr Sci, Zhengzhou, Henan, Peoples R China;[Guan, Chun-Yun] Natl Ctr Oilseed Crop Improvement, Hunan Branch, Changsha, Hunan, Peoples R China
通讯机构：
[Hua, Ying-Peng] H;[Hua, Ying-Peng] Z;Hunan Agr Univ, Coll Resources & Environm Sci, Southern Reg Collaborat Innovat Ctr Grain & Oil C, Changsha, Hunan, Peoples R China. Zhengzhou Univ, Sch Agr Sci, Zhengzhou, Henan, Peoples R China.
关键词：
Allotetraploid rapeseed;plant cadmium resistance;cadmium toxicity;multiomics;genotypic diversity;subcellular reallocation
摘要：
Oilseed rape (Brassica napus) has great potential for phytoremediation of cadmium (Cd)-polluted soils due to its large plant biomass production and strong metal accumulation. Enhanced plant Cd resistance (PCR) is a crucial prerequisite for phytoremediation through hyper-accumulation of excess Cd. However, the complexity of the allotetraploid genome of rapeseed hinders our understanding of PCR. To explore rapeseed Cd-resistance mechanisms, we examined two genotypes, 'ZS11' (Cd-resistant) and 'W10' (Cd-sensitive), that exhibit contrasting PCR while having similar tissue Cd concentrations, and characterized their different fingerprints in terms of plant morphophysiology (electron microscopy), ion abundance (inductively coupled plasma mass spectrometry), DNA variation (whole-genome resequencing), transcriptomics (high-throughput mRNA sequencing), and metabolomics (ultra-high performance liquid chromatography-mass spectrometry). Fine isolation of cell components combined with ionomics revealed that more Cd accumulated in the shoot vacuoles and root pectins of the resistant genotype than in the sensitive one. Genome and transcriptome sequencing identified numerous DNA variants and differentially expressed genes involved in pectin modification, ion binding, and compartmentalization. Transcriptomics-assisted gene co-expression networks characterized BnaCn.ABCC3 and BnaA8.PME3 as the central members involved in the determination of rapeseed PCR. High-resolution metabolic profiles revealed greater accumulation of shoot Cd chelates, and stronger biosynthesis and higher demethylation of root pectins in the resistant genotype than in the sensitive one. Our comprehensive examination using a multiomics approach has greatly improved our understanding of the role of subcellular reallocation of Cd in the determination of PCR. © 2019 The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology.
语种：
英文
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甘蓝型油菜AVP1、VHA-a2和VHA-a3基因的鉴定及功能性研究
作者：
姚珺玥;华营鹏;周婷;王涛;宋海星;...
期刊：
作物学报 ,2019年45(8):1146-1157 ISSN：0496-3490
通讯作者：
Zhang, Z.-H.
作者机构：
湖南农业大学资源环境学院, 湖南, 长沙, 410128;南方粮油作物协同创新中心, 南方粮油作物协同创新中心, 湖南, 长沙, 410128;国家油料作物改良中心湖南分中心, 国家油料作物改良中心湖南分中心, 湖南, 长沙, 410128;[华营鹏; 周婷; 王涛; 宋海星; 姚珺玥; 张振华] College of Resource and Environment, Hunan Agricultural University, Changsha, Hunan 410128, China, Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China, Changsha, Hunan 410128, China;[官春云] Hunan Branch, National Oil Crops Improvement Center, Changsha, Hunan 410128, China
通讯机构：
[Zhang, Z.-H.] C;College of Resource and Environment, China
关键词：
甘蓝型油菜;AVP基因;VHA基因;生物信息学
摘要：
液泡是调控植物细胞分化、生长发育的重要部位, AVP1、VHA-a2和VHA-a3基因调控植物液泡内外离子平衡、离子运输以及能量供应。本研究利用功能已知的拟南芥AVP1、VHA-a2和VHA-a3基因为参考序列在甘蓝型油菜全基因组数据库、NCBI植物基因组注释数据库等鉴定并筛选出9个BnaAVP1、3个BnaVHA-a2和4个BnaVHA-a3,并分析基因拷贝数变异、分子特征、跨膜结构域、保守基序、染色体定位、系统进化树构建、蛋白二级结构及三维结构预测、高通量转录组测序等。发现甘蓝型油菜BnaAVP1和BnaVHA-a3的基因数量明显多于甘蓝和白菜;甘蓝型油菜AVP1、VHA-a2和VHA-a3蛋白属于由酸性氨基酸组成的稳定蛋白;系统进化选择能力的分析表明,甘蓝型油菜AVP1、VHA-a2和VHA-a3家族基因与甘蓝、白菜关系相近。转录组测序表明,低氮处理后, BnaAVP1s基因主要在地上部表达,且低氮3 h后地上部表达下调,低氮处理72 h根中表达量上调; BnaVHA-a2s和BnaVHA-a3s基因在地上部和根中均有表达, BnaVHA-a2s在低氮处理72 h后表达量基本呈上调趋势, BnaVHA-a3s在低氮3 h后基本呈下调趋势。低磷处理后, BnaAVP1s根中大部分基因表达上调,地上部表达基本无差异; BnaVHA-a2s表达基本无差异; BnaVHA-a3s地上部和根中均基本为上调趋势。该结果为进一步研究甘蓝型油菜AVP1、VHA-a2和VHA-a3基因生物学功能及AVP1、VHA-a2和VHA-a3蛋白水解ATP提供能量供植物代谢的分子机制奠定基础,为已知大量数据的其他物种家族基因生物信息学研究提供参考。
语种：
中文
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低氮密植对机插晚稻产量形成和光合特性的影响
作者：
杨志长;沈涛;胡宇倩;宋海星
期刊：
南方农业学报 ,2019年50(3):546-553 ISSN：2095-1191
作者机构：
湖南农业大学资源环境学院,长沙,410128;[胡宇倩; 宋海星; 沈涛; 杨志长] 湖南农业大学
关键词：
机插晚稻;低氮密植;常规栽培;产量;光合特性
摘要：
【目的】探明低氮密植对机插晚稻产量形成和光合特性的影响,为推广低氮密植栽培技术提供理论依据。 【方法】在大田试验条件下,采用裂区设计,以杂交稻泰优390和常规稻湘晚籼13为材料,设低氮高密(N 120 kg/ha、 36.36万穴/ha)和常规栽培(N 150 kg/ha、28.57万穴/ha)2种氮肥密度水平,测定不同氮肥密度处理下机插晚稻的产量及其构成因素、茎蘖动态、干物质含量、主要生育时期叶面积指数和叶绿素相对含量(SPAD值)及齐穗期叶片的光合特性参数。【结果】与常规栽培相比,低氮高密处理下泰优390产量降低5.56%,但差异不显著(P>0.05,下同),湘晚籼13产量无显著变化;低氮高密处理机插晚稻的有效穗数、单位面积茎蘖数、成穗率、收获指数和生育中后期群体生长率上升,群体干物重和叶面积指数无显著变化;泰优390生育中后期叶片SPAD值和净光合速率(Pn)显著下降(P<0.05),湘晚籼13生育中后期叶片SPAD值和Pn无显著变化。【结论】在氮肥施用量减少20%的条件下(150 kg/ha降至120 kg/ha)适当增加种植密度,机插晚稻仍可稳产。综合考虑产量、肥料成本和环境保护,低氮密植栽培技术值得推广。
语种：
中文
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低氮密植栽培对机插晚稻产量及氮素吸收利用的影响
作者：
沈涛;杨志长;胡宇倩;宋海星
期刊：
西北农业学报 ,2019年28(5):729-734 ISSN：1004-1389
作者机构：
湖南农业大学资源环境学院/土壤肥料资源高效利用国家工程实验室/农田污染控制与农业资源利用湖南省重点实验室/植物营养湖南省普通高等学校重点实验室,长沙,410128;[胡宇倩; 宋海星; 杨志长; 沈涛] 植物营养湖南省普通高等学校
关键词：
晚稻;低氮密植;产量;氮素利用率
摘要：
研究低氮密植栽培对机插晚稻干物质累积与氮素利用效率的影响,为水稻高产高效栽培提供依据,以杂交稻‘泰优390’与常规稻‘湘晚籼13’为材料,在大田栽培条件下,研究机插密度(36.4穴/m~2、28.6穴/m~2)与施氮肥量(0、120、150kg/hm~2)对机插晚稻产量及氮素利用率的影响。结果表明:与常规栽培(28.6穴/m~2,150kg/hm~2)相比,低氮密植(36.4穴/m~2,120kg/hm~2)条件下,晚稻的干物质累积量与氮素累积量显著提升,‘泰优390’与‘湘晚籼13’的收获期干物质累积量分别提高22.8%和15.3%,氮素累积量分别提高10. 6%和16.2%;产量构成因素中,穗粒数、结实率、千粒质量在2种栽培条件下无显著差异,但有效穗数、偏生产力及氮肥吸收利用率显著提高。由此可见,水稻低氮密植栽培保持了稳定高产,同时显著提高氮肥利用率,是一种可实现水稻高产高效的栽培模式。
语种：
中文
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甘蓝型油菜NRT1.5和NRT1.8家族基因的生物信息学分析及其对氮-镉胁迫的响应
作者：
梁桂红;华营鹏;周婷;廖琼;宋海星;...
期刊：
作物学报 ,2019年45(3):365-380 ISSN：0496-3490
通讯作者：
Zhang, Z.-H.
作者机构：
湖南农业大学资源环境学院, 湖南长沙 410128;南方粮油作物协同创新中心, 湖南长沙 410128;[梁桂红; 华营鹏; 廖琼; 宋海星; 张振华; 周婷] College of Resource and Environment, Hunan Agricultural University, Changsha, Hunan 410128, China, Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China, Changsha, Hunan 410128, China
通讯机构：
[Zhang, Z.-H.] C;College of Resource and Environment, China
关键词：
甘蓝型油菜;生物信息学;硝酸盐;镉
摘要：
植物对硝酸盐的吸收和转运需要硝酸盐转运体(nitrate transporters, NRTs)的协助。在拟南芥中,硝酸盐的长途转运及其在根部和地上部的分配,主要受NRT1 家族的两个成员NRT1.5 和NRT1.8 的协同调控,且两者的表达均受到硝酸盐的强烈诱导。本文以AtNRT1.5 和AtNRT1.8 基因序列为基础序列,采用生物信息学方法鉴定了白菜、甘蓝和甘蓝型油菜中NRT1.5 和NRT1.8 同源基因,并对基因结构和分子特性、基因拷贝数变异、基因染色体分布、系统进化树、蛋白保守序列比对和跨膜结构域、基因响应低氮和镉胁迫的转录组测序以及基因共表达网络进行了分析。结果表明,白菜、甘蓝及甘蓝型油菜中NRT1.5 和NRT1.8 蛋白均含有保守的跨膜结构域和保守基序(F-Y-L-A-L-N-LG-S-L),属于MFS (major facilitator superfamily)超家族的小肽转运体PTR (peptide transporter)家族。转录组测序结果表明,甘蓝型油菜低氮处理72 h,根部NRT1.5 基因的表达丰度上调而抑制NRT1.8 的表达;镉处理条件下,乙烯/茉莉酸-硝酸盐转运体介导的信号途径能够促进NRT1.8 表达上调而抑制NRT1.5 的表达,从而使更多的硝酸盐从地上部运输到根部,提高植物抗镉胁迫的能力。本研究为进一步了解甘蓝型油菜NRT1.5 和NRT1.8 家族基因的生物学功能及其对氮-镉胁迫的响应奠定基础,同时为NRT1.5 和NRT1.8 家族基因在其他物种中的生物信息学研究提供参考。
语种：
中文
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生态沟渠吸收氮磷效果研究
作者：
杨勇;田昌;谢桂先;张玉平;宋海星;...
期刊：
湖南农业科学 ,2019年(1):39-42 ISSN：1006-060X
作者机构：
湖南农业大学资源环境学院,南方粮油作物协同创新中心,土壤肥料资源高效利用国家工程实验室,农田污染控制与农业资源利用湖南省重点实验室,植物营养湖南省普通高等学校重点实验室,湖南 长沙 410128;[谢桂先; 荣湘民; 张玉平; 田昌; 杨勇; 宋海星] 湖南农业大学
关键词：
水生植物;生态拦截;生态沟渠
摘要：
采用模拟沟渠试验,研究了9种水生植物吸收氮磷能力的差异,并选择氮磷吸收能力较好品种构建生态沟渠和氧化塘,研究其氮磷吸收效果。结果表明,种植水生植物后模拟沟渠水中氮磷残留量显著减少,美人蕉、狐尾藻、珍珠梅、海寿花和茭白的生物量高,氮磷的吸收量大,其中种植美人蕉和狐尾藻效果最佳。农田排水中氮磷通过生态沟渠水生植物吸收后浓度显著降低;主沟总氮、可溶性氮和总磷浓度平均降低39.6%、40.1%和36.9%;支沟总氮、可溶性氮和总磷浓度可再平均降低6.6%、10.3%和13.6%;氧化塘总氮和总磷浓度可再降低17.7%和13.3%。生态沟渠可有效拦截水体氮磷,具有净化水质的良好生态效益。
语种：
中文
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油菜不同生长期稻田土壤无机氮形态及氮肥利用率对控释氮肥施用的响应
作者：
廖佳元;宋海星;赵东生;王涛;官春云;...
期刊：
水土保持学报 ,2019年33(1):158-164 ISSN：1009-2242
作者机构：
湖南农业大学资源环境学院,南方粮油作物协同创新中心,土壤肥料资源高效利用国家工程实验室,农田污染控制与农业资源利用湖南省重点实验室,植物营养湖南省普通高等学校重点实验室,长沙410128;[官春云; 廖佳元; 王涛; 张振华; 赵东生; 宋海星] 湖南农业大学
关键词：
油菜;控释氮肥;氮素形态;产量;氮肥利用率
摘要：
通过盆栽土培试验研究了尿素、控释氮肥对南方稻田冬油菜生长、产量、土壤肥效和氮肥利用效率的影响,为控释氮肥在油菜生产上的推广应用提供参考。试验选用油菜品种“湘油15”,参考油菜大田种植施氮量,共设4个处理,以不施氮肥(CK)为对照,在施氮量均为200kg/hm~2的水平下,设置了3种氮肥处理:尿素(Urea)、控释氮肥1(CRNF1)和控释氮肥2(CRNF2)。对油菜生物量和产量、不同生育期下土壤无机氮、油菜氮素吸收、油菜生理特性、土壤微生物氮以及土壤酶活性等相关指标进行测定及分析。结果表明:较常规Urea处理相比,控释氮肥处理显著提高了油菜花期、收获期生物量,增产11.2%~20.1%;CRNF1处理显著提高了油菜花期、收获期土壤NO_3~--N含量,相比尿素处理分别提高43.2%和61.8%,CRNF2处理显著提高了油菜花期、收获期土壤NH_4~+-N含量,相比尿素处理分别提高18.7%和64.1%,保证了油菜生育后期土壤氮素供应;与Urea处理相比,控释氮肥显著提高了油菜薹期及生殖生长期油菜总氮吸收,最终氮肥利用率(NUE)提高23.1%~60.2%,氮肥农学利用率(NAE)提高19.1%~30.5%;CRNF1处理显著提高了油菜生长后期SPDA值和总叶绿素含量,相比尿素处理分别提高6.5%,10.1%;CRNF1处理极显著提高了油菜生长后期土壤微生物氮,较尿素处理提高142.5%;此外,控释氮肥显著提高了油菜生长后期土壤脲酶、FDA水解酶活性,相比尿素处理分别提高8.4%~12.9%,24.5%~32.4%。在总氮施用量不变的前提下,施用控释氮肥可提高油菜生殖生长期土壤有效氮含量,改善光合作用,增强土壤微生物量和微生物活性,促进氮素的吸收,提高氮肥的利用效率,进而增加油菜干物质累积,最终提高产量。
语种：
中文
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Long-term rice-rice-rape rotation optimizes 1,2-benzenediol concentration in rhizosphere soil and improves nitrogen-use efficiency and rice growth
作者：
Lu, Sheng;Song, Haixing;Guan, Chunyun;Lepo, Joe Eugene;Wu, Zhimin;...
期刊：
Plant and Soil ,2019年445(1-2):23-37 ISSN：0032-079X
通讯作者：
Zhang, Zhenhua;He, Xinhua
作者机构：
[Song, Haixing; Zhang, Zhenhua; Lu, Sheng; Wu, Zhimin] Hunan Agr Univ, Coll Resources & Environm Sci, Southern Reg Collaborat Innovat Ctr Grain & Oil C, Changsha 410128, Peoples R China.;[Guan, Chunyun] Natl Ctr Oilseed Crops Improvement, Hunan Branch, Changsha 410128, Peoples R China.;[Lepo, Joe Eugene] Univ West Florida, Ctr Environm Diagnost & Bioremediat, Pensacola, FL 32514 USA.;[He, Xinhua] Southwest Univ, Coll Nat Resources & Environm, Chongqing 400715, Peoples R China.;[He, Xinhua] Univ Western Australia, Sch Biol Sci, Crawley, WA 6009, Australia.
通讯机构：
[Zhang, Zhenhua] H;[He, Xinhua] U;Hunan Agr Univ, Coll Resources & Environm Sci, Southern Reg Collaborat Innovat Ctr Grain & Oil C, Changsha 410128, Peoples R China.;Univ Western Australia, Sch Biol Sci, Crawley, WA 6009, Australia.
关键词：
Allelochemical metabolite;Brassica napus;Glutamine synthetase;Nitrate reductase;Oryza sativa
摘要：
Aims: We examined differences in soil metabolites from the rice root rhizosphere of long-term rice-rice-fallow (RRF) and rice-rice-rape (RRR) rotations, and examined the effects of 1,2-benzenediol on nitrogen-use efficiency (NUE) and rice growth. Methods: The metabolite composition of rice rhizospheres was analyzed using the gas chromatography-mass spectrometry (GC-MS). A range of 0.2, 2.0 and 200 μmol L−1 concentrations of external 1,2-benzenediol were applied to examine their effects on rice growth, nitrate reductase (NR) and glutamine synthetase (GS) activities, and physiological nitrogen-use efficiency (PNUE). Results: The metabolite composition of rhizospheres differed significantly between RRR and RRF. Soil total N and 1,2-benzenediol concentrations during the early rice season were significantly lower under RRR than RRF. Rice growth and NUE significantly enhanced at 0.20 μmol 1,2-benzenediol L−1, but inhibited at 2.0 μmol L−1 or higher. Changes in root morphology and uptake associated with 1,2-benzenediol possibly had contributed to a higher NUE of the early season rice under RRR. The NR and GS activities in rice roots were significantly higher with 0.2 μmol L−1 1,2-benzenediol than without 1,2-benzenediol treatment. Conclusions: Crop rotation significantly affected rice rhizosphere metabolites. An optimal soil 1,2-benzenediol concentration under long-term RRR rotation may be associated with an enhanced NUE and root N uptake and assimilation, resulting in an increased rice growth and yield. © 2019, Springer Nature Switzerland AG.
语种：
英文
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Integrated physiologic, genomic and transcriptomic strategies involving the adaptation of allotetraploid rapeseed to nitrogen limitation
作者：
Zhang, Zhen-hua;Zhou, Ting;Liao, Qiong;Yao, Jun-yue;Liang, Gui-hong;...
期刊：
BMC Plant Biology ,2018年18(1):1-18 ISSN：1471-2229
通讯作者：
Hua, Ying-peng
作者机构：
[Zhang, Zhen-hua; Yao, Jun-yue; Liao, Qiong; Liang, Gui-hong; Zhou, Ting; Song, Hai-xing; Hua, Ying-peng] Hunan Agr Univ, Coll Resources & Environm Sci, Southern Reg Collaborat Innovat Ctr Grain & Oil C, Changsha, Hunan, Peoples R China.;[Guan, Chun-yun] Natl Ctr Oilseed Crop Improvement, Hunan Branch, Changsha, Hunan, Peoples R China.
通讯机构：
[Hua, Ying-peng] H;Hunan Agr Univ, Coll Resources & Environm Sci, Southern Reg Collaborat Innovat Ctr Grain & Oil C, Changsha, Hunan, Peoples R China.
关键词：
Allotetraploid rapeseed;Genomic selection;BnamiR827-BnaNLA1s-BnaNRT1.7 s;Nitrogen limitation adaptation;Nitrogen use efficiency;Transcriptional profiling
摘要：
Nitrogen (N) is a macronutrient that is essential for optimal plant growth and seed yield. Allotetraploid rapeseed (AnAnCnCn, 2n = 4x = 38) has a higher requirement for N fertilizers whereas exhibiting a lower N use efficiency (NUE) than cereal crops. N limitation adaptation (NLA) is pivotal for enhancing crop NUE and reducing N fertilizer use in yield production. Therefore, revealing the genetic and molecular mechanisms underlying NLA is urgent for the genetic improvement of NUE in rapeseed and other crop species with complex genomes. In this study, we integrated physiologic, genomic and transcriptomic analyses to comprehensively characterize the adaptive strategies of oilseed rape to N limitation stresses. Under N limitations, we detected accumulated anthocyanin, reduced nitrate (NO3−) and total N concentrations, and enhanced glutamine synthetase activity in the N-starved rapeseed plants. High-throughput transcriptomics revealed that the pathways associated with N metabolism and carbon fixation were highly over-represented. The expression of the genes that were involved in efficient N uptake, translocation, remobilization and assimilation was significantly altered. Genome-wide identification and molecular characterization of the microR827-NLA1-NRT1.7 regulatory circuit indicated the crucial role of the ubiquitin-mediated post-translational pathway in the regulation of rapeseed NLA. Transcriptional analysis of the module genes revealed their significant functional divergence in response to N limitations between allotetraploid rapeseed and the model Arabidopsis. Association analysis in a rapeseed panel comprising 102 genotypes revealed that BnaC5.NLA1 expression was closely correlated with the rapeseed low-N tolerance. We identified the physiologic and genome-wide transcriptional responses of oilseed rape to N limitation stresses, and characterized the global members of the BnamiR827-BnaNLA1s-BnaNRT1.7s regulatory circuit. The transcriptomics-assisted gene co-expression network analysis accelerates the rapid identification of central members within large gene families of plant species with complex genomes. These findings would enhance our comprehensive understanding of the physiologic responses, genomic adaptation and transcriptomic alterations of oilseed rape to N limitations and provide central gene resources for the genetic improvement of crop NLA and NUE.
语种：
英文
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纳米膨润土包膜氮肥对晚稻产量与氮素利用率的影响
作者：
李旭霞;宋海星;廖琼;沈涛
期刊：
中国农学通报 ,2018年34(11):81-85 ISSN：1000-6850
作者机构：
湖南农业大学资源环境学院,农田污染控制与农业资源利用湖南省重点实验室,土壤肥料资源高效利用国家工程实验室,植物营养湖南省普通高等学校重点实验室,长沙410128;[宋海星; 沈涛; 廖琼; 李旭霞] 湖南农业大学
关键词：
纳米膨润土;施氮水平;产量;氮素利用率
摘要：
采用大田试验,研究了不同比例纳米膨润土包膜氮肥及不同施氨水平对晚稻生长、产量和氮素利用率的影响.结果表明:施用纳米膨润土包膜氮肥处理的产量、千粒重、穗粒数等均显著高于空白处理(CK),其中施用氮肥处理比CK处理的产量高出21.53％～28.01％ (P＜ 0.05),不同施氮水平下施用纳米膨润土包膜氮肥对晚稻产量差异有影响,10％纳米膨润土包膜氮肥在不减氮、减氮20％及减氮30％时,产量分别为7178.54、7220.28、7236.95 kg/hm2,与常规氮肥处理(7095.21 kg/hm2)相比,增加了1.17％、1.76％和1.99％,差异均不显著(P＞0.05),15％纳米膨润土包膜氮肥不减氮时产量为7270.30 kg/hm2,增加2.47％(P＞ 0.05),减氮20％～30％时减产0.12％～3.17％(P＞0.05);施用纳米膨润土包膜氮肥的处理,结实率均低于常规氮肥处理,差异不显著(P＞0.05);纳米膨润土包膜氮肥在减氮条件下,SPAD值无显著差异(P＞0.05).成熟期籽粒中氮素累积量,施氮肥处理极显著高于不施肥处理,且以15％纳米膨润土包膜氮肥减氮20％时氮素积累量最高(68.92 kg N/hm2);纳米膨润土包膜氮肥的氮肥利用率(49.3％～67.1％)高于常规氮肥处理(46.5％) 2.8％～20.6％,氮肥利用率均有所增加,综合产量、结实率和氮肥利用率等考虑,10％纳米膨润土减氮20％的效果更佳.
语种：
中文
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NRT1.1-Related NH4+ Toxicity Is Associated with a Disturbed Balance between NH4+ Uptake and Assimilation
作者：
Jian, Shaofen;Liao, Qiong;Song, Haixing;Liu, Qiang;Lepo, Joe Eugene;...
期刊：
PLANT PHYSIOLOGY ,2018年178(4):1473-1488 ISSN：0032-0889
通讯作者：
Zhang, Zhenhua
作者机构：
[Song, Haixing; Zhang, Zhenhua; Liao, Qiong; Liu, Qiang; Jian, Shaofen] Hunan Agr Univ, Coll Resources & Environm Sci, Southern Reg Collaborat Innovat Ctr Grain & Oil C, Changsha 410128, Hunan, Peoples R China.;[Lepo, Joe Eugene] Univ West Florida, Ctr Environm Diagnost & Bioremediat, Pensacola, FL 32514 USA.;[Guan, Chunyun] Natl Ctr Oilseed Crops Improvement, Hunan Branch, Changsha 410128, Hunan, Peoples R China.;[Zhang, Jianhua] Hong Kong Baptist Univ, Dept Biol, SCT704, Hong Kong, Hong Kong, Peoples R China.;[Ismail, Abdelbagi M.] Int Rice Res Inst, DAPO 7777, Manila, Philippines.
通讯机构：
[Zhang, Zhenhua] H;Hunan Agr Univ, Coll Resources & Environm Sci, Southern Reg Collaborat Innovat Ctr Grain & Oil C, Changsha 410128, Hunan, Peoples R China.
摘要：
A high concentration of ammonium (NH4+) as the sole source of nitrogen in the growth medium often is toxic to plants. The nitrate transporter NRT1.1 is involved in mediating the effects of NH4+ toxicity; however, the mechanism remains undefined. In this study, wild-type Arabidopsis (Arabidopsis thaliana Columbia-0 [Col-0]) and NRT1.1 mutants (chl1-1 and chl1-5) were grown hydroponically in NH4NO3 and (NH4)(2)SO4 media to assess the function of NRT1.1 in NH4+ stress responses. All the plants grew normally in medium containing mixed nitrogen sources, but Col-0 displayed more chlorosis and lower biomass and photosynthesis than the NRT1.1 mutants in (NH4)(2)SO4 medium. Grafting experiments between Col-0 and chli-5 further confirmed that NH4+ toxicity is influenced by NRT1.1. In (NHASO, medium, NRT1.1 induced the expression of NH4+ transporters, increasing NH4+ uptake. Additionally, the activities of glutamine synthetase and glutamate synthetase in roots of Col-0 plants decreased and soluble sugar accumulated significantly, whereas pyruvate kinase-mediated glycolysis was not affected, all of which contributed to NH4+ accumulation. By contrast, the NRT1.1 mutants showed reduced NH4+ accumulation and enhanced NH4+ assimilation through glutamine synthetase, glutamate synthetase, and glutamate dehydrogenase. Moreover, the up-regulation of genes involved in ethylene synthesis and senescence in Col-0 plants treated with (NH4)(2)SO4 suggests that ethylene is involved in NH4+ toxicity responses. This study showed that NH4+ toxicity is related to a nitrate-independent signaling function of NRT1.1 in Arabidopsis, characterized by enhanced NH4+ accumulation and altered NH4+ metabolism, which stimulates ethylene synthesis, leading to plant senescence.
语种：
英文
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生物质材料与营养物配施对石油污染土壤的修复
作者：
王艳杰;李法云;荣湘民;陈佳勃;石丽芳
期刊：
农业环境科学学报 ,2018年37(2):232-238 ISSN：1672-2043
作者机构：
[荣湘民] School of Resources and Environmental Science, Hunan Agricultural University, Changsha, 410128, China;[陈佳勃; 石丽芳] Institute of Eco-environmental Sciences, Liaoning Shihua University, Fushun, 113001, China;[王艳杰; 李法云] School of Resources and Environmental Science, Hunan Agricultural University, Changsha, 410128, China<&wdkj&>Institute of Eco-environmental Sciences, Liaoning Shihua University, Fushun, 113001, China
关键词：
石油烃;生物炭;营养物;微生物
摘要：
为研究营养物质、玉米秸秆碎屑和生物炭材料对石油污染土壤修复效果的影响,对4个处理组修复后土壤理化性质、修复中和修复后土壤生物特征和石油烃类物质的去除率,以及上述指标的相关关系进行分析。结果表明:添加玉米秸秆碎屑和生物炭能够显著降低土壤容重,增加土壤孔隙度,并提高土壤田间持水量(P<0.05)。在石油污染土壤中添加营养物和两种疏松材料均能提高土壤中微生物数量,其中生物炭与营养物联合处理组效果最佳,修复90 d微生物数量达到7.24×10~7 CFU·g~(-1)。修复后玉米秸秆碎屑与营养物联合处理组和生物炭与营养物联合处理组总石油烃和不同组分烃类物质明显减少,尤其是生物炭联合处理组,明显高于单独营养物处理组和对照组。土壤理化性质、微生物数量、石油烃去除率三者之间多呈显著相关,其中土壤孔隙度和总氮对微生物影响较大,微生物对石油烃类的去除作用明显。因此,添加生物炭材料并配施营养物质既可以改善土壤物理性质又能够为微生物提供充足的养分,进而增强石油污染土壤的修复效果。
语种：
中文
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Increased rice yield in long-term crop rotation regimes through improved soil structure, rhizosphere microbial communities, and nutrient bioavailability in paddy soil
作者：
Lu, Sheng;Lepo, Joe Eugene;Song, Hai-Xing;Guan, Chun-Yun;Zhang, Zhen-Hua*
期刊：
Biology and Fertility of Soils ,2018年54(8):909-923 ISSN：0178-2762
通讯作者：
Zhang, Zhen-Hua
作者机构：
[Song, Hai-Xing; Lu, Sheng; Zhang, Zhen-Hua] Hunan Agr Univ, Natl Engn Lab Efficient Utilizat Soil & Fertilize, Southern Reg Collaborat Innovat Ctr Grain & Oil C, Coll Resources & Environm Sci,Hunan Prov Key Lab, Changsha, Hunan, Peoples R China.;[Lepo, Joe Eugene] Univ West Florida, Environm Diagnost & Bioremediat, Pensacola, FL 32514 USA.;[Guan, Chun-Yun] Natl Ctr Oilseed Crops Improvement, Hunan Branch, Changsha, Hunan, Peoples R China.
通讯机构：
[Zhang, Zhen-Hua] H;Hunan Agr Univ, Natl Engn Lab Efficient Utilizat Soil & Fertilize, Southern Reg Collaborat Innovat Ctr Grain & Oil C, Coll Resources & Environm Sci,Hunan Prov Key Lab, Changsha, Hunan, Peoples R China.
关键词：
Crop rotation;Soil nutrition;Soil organic matter;Soil microbes;Early rice yield
摘要：
Soil in short-term crop rotation systems (STCR) is still in the initial development stage of farmland soil, whereas after long-term crop rotation treatment (LTCR), soil properties are significantly different. This study compares STCR (4years) and LTCR (30years) rice-rice-fallow, rice-rice-rape rotation practices under the same soil type background and management system. To reveal ecosystem mechanisms within soils and their effects on rice yield following LTCR, we analyzed the physical, chemical, and microbiological properties of soils with different rotations and rotation times. Relative to STCR, LTCR significantly reduced soil water-stable aggregate (WSA) content in the < 0.053-mm range, while > 2mm WSA content significantly increased. Soil organic matter increased in fields under LTCR, mainly in > 2mm, 2–0.25mm, and < 0.053mm soil WSA in 0–10cm soil layer. LTCR was associated with significantly increased total soil organic matter, at the same time being associated with increasing the amount of active organic matter in the 0–20cm soil layer. The two crop rotation regimes significantly differed in soil aggregate composition as well as in soil N and P, microbial biomass, and community composition. Relative to STCR, LTCR field soils had significantly higher soil organic matter, active organic matter content, soil enzyme activities, and overall microbial biomass, while soil WSA and microbial community composition was significantly different. Our results demonstrate that LTCR could significantly improve soil quality and rice yield and suggest that length of rotation time and rice-rice-rape rotation are critical factors for the development of green agriculture. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.
语种：
英文
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Genome-scale characterization of the vacuole nitrate transporter Chloride Channel (CLC) genes and their transcriptional responses to diverse nutrient stresses in allotetraploid rapeseed
作者：
Liao, Qiong;Zhou, Ting;Yao, Jun-yue;Han, Qing-fen;Song, Hai-xing;...
期刊：
PLOS ONE ,2018年13(12):e0208648 ISSN：1932-6203
通讯作者：
Hua, Ying-peng;Zhang, Zhen-hua
作者机构：
[Han, Qing-fen; Zhang, Zhen-hua; Yao, Jun-yue; Hua, Ying-peng; Liao, Qiong; Zhou, Ting; Song, Hai-xing] Hunan Agr Univ, Southern Reg Collaborat Innovat Ctr Grain & Oil C, Coll Resources & Environm Sci, Changsha, Hunan, Peoples R China.;[Guan, Chun-yun] Natl Ctr Oilseed Crops Improvement, Hunan Branch, Changsha, Hunan, Peoples R China.
通讯机构：
[Hua, YP; Zhang, ZH] H;Hunan Agr Univ, Southern Reg Collaborat Innovat Ctr Grain & Oil C, Coll Resources & Environm Sci, Changsha, Hunan, Peoples R China.
关键词：
Brassica;Arabidopsis thaliana;Plant vacuoles;Sequence motif analysis;Crops;Rapeseed;Crop genetics;Gene regulation
摘要：
The Chloride Channel (CLC) gene family is reported to be involved in vacuolar nitrate (NO 3 - ) transport. Nitrate distribution to the cytoplasm is beneficial for enhancing NO 3 - assimilation and plays an important role in the regulation of nitrogen (N) use efficiency (NUE). In this study, genomic information, high-throughput transcriptional profiles, and gene co-expression analysis were integrated to identify the CLCs (BnaCLCs) in Brassica napus. The decreased NO 3 - concentration in the clca-2 mutant up-regulated the activities of nitrate reductase and glutamine synthetase, contributing to increase N assimilation and higher NUE in Arabidopsis thaliana. The genome-wide identification of 22BnaCLC genes experienced strong purifying selection. Segmental duplication was the major driving force in the expansion of the BnaCLC gene family. The most abundant cis-acting regulatory elements in the gene promoters, including DNA-binding One Zinc Finger, W-box, MYB, and GATA-box, might be involved in the transcriptional regulation of BnaCLCs expression. High-throughput transcriptional profiles and quantitative real-time PCR results showed that BnaCLCs responded differentially to distinct NO 3 - regimes. Transcriptomics-assisted gene co-expression network analysis identified BnaA7.CLCa-3 as the core member of the BnaCLC family, and this gene might play a central role in vacuolar NO 3 - transport in crops. The BnaCLC members also showed distinct expression patterns under phosphate depletion and cadmium toxicity. Taken together, our results provide comprehensive insights into the vacuolar BnaCLCs and establish baseline information for future studies on BnaCLCs-mediated vacuolar NO 3 - storage and its effect on NUE. Copyright: © 2018 Liao et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
语种：
英文
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