Lotus seed skin proanthocyanidin extract exhibits potent antioxidant property via activation of the Nrf2-ARE pathway
作者：
Li, Tao;Li, Qili;Wu, Weiguo;Li, Yong;Hou, De-xing;...
期刊：
生物化学与生物物理学报 ,2018年51(1):31-40 ISSN：1672-9145
通讯作者：
Lu, Xiangyang;Deng, Fangming;Qin, Si
作者机构：
[Lu, Xiangyang; Qin, Si; Li, Yong; Li, Tao; Deng, Fangming; Wu, Weiguo; Hou, De-xing; Lu, XY; Qin, S] Hunan Agr Univ, Coll Food Sci & Technol, Key Lab Food Sci & Biotechnol Hunan Prov, Core Res Program 1515, Changsha 410128, Hunan, Peoples R China.;[Li, Qili; Shan, Yang] Hunan Acad Agr Sci, Hunan Agr Prod Proc Inst, Changsha 410125, Hunan, Peoples R China.;[Qin, Si; Hou, De-xing] Kagoshima Univ, United Grad Sch Agr Sci, Fac Agr, Kagoshima 8900065, Japan.;[Xu, Hua; Qin, Si] Univ Arizona, Dept Pediat, Steele Childrens Res Ctr, Tucson, AZ 85724 USA.;[Zheng, Baodong; Zeng, Shaoxiao] Fujian Agr & Forestry Univ, Dept Food Safety, Coll Food Sci, Fuzhou 350002, Fujian, Peoples R China.
通讯机构：
[Lu, XY; Deng, FM; Qin, S] H;[Qin, Si] K;[Qin, Si] U;Hunan Agr Univ, Coll Food Sci & Technol, Key Lab Food Sci & Biotechnol Hunan Prov, Core Res Program 1515, Changsha 410128, Hunan, Peoples R China.;Kagoshima Univ, United Grad Sch Agr Sci, Fac Agr, Kagoshima 8900065, Japan.
关键词：
lotus seed skin extract;proanthocyanidin;Nrf2-ARE pathway;antioxidant activity
摘要：
Lotus seed is well known as traditional food and medicine, but its skin is usually discarded. Recent studies have shown that lotus seed skin contains a high concentration of proanthocyanidins that have multi-functions, such as antioxidation, anti-inflammation, and anti-cancer effects. In the present study, we aimed to isolate and purify the proanthocyanidins from lotus seed skin by acetone extraction and rotary evaporation, identify their chemical structures by HPLC-MS-MS and NMR, and further investigate the antioxidant properties of the extract purified by macroporous resin (PMR) from lotus seed skin both in vitro and in vivo. The results showed that PMR mainly contained oligomeric proanthocyanidins, especially dimeric procyanidin B1 (PB1), procyanidin B2 and procyanidin B4. Although it had limited ability to directly scavenge radicals in vitro, PMR could significantly enhance the expressions of antioxidant proteins via activation of nuclear factor-E2-related factor 2 (Nrf2)-antioxidant response element (ARE) pathway in HepG2 cells. Molecular data revealed that PB1, a major component in PMR, stabilized Nrf2 by inhibiting the ubiquitination of Nrf2, which led to subsequent activation of the Nrf2-ARE pathway, including the enhancements of Nrf2 nuclear translocation, Nrf2-ARE binding and ARE transcriptional activity. Moreover, the in vivo results in high fat diet-induced mice further verified the powerful antioxidant property of PMR. These results revealed that lotus seed skin is a promising resource for functional food development.
语种：
英文
展开
曲靖烤烟钾含量特征及其与主要生态因子关系研究
作者：
李强;张一扬;程昌新;刘华林;王瑞宝;...
期刊：
核农学报 ,2017年31(5):918-926 ISSN：1000-8551
作者机构：
[李强] 湖南农业大学食品科学技术学院,湖南长沙410128;[李强] 湖南农业大学烟草研究院,湖南长沙410128;湖南农业大学烟草研究院,湖南长沙,410128;红云红河烟草(集团)有限责任公司,云南昆明,650231;云南省烟草公司曲靖市公司,云南曲靖,655000
关键词：
烤烟;钾含量;生态因子;曲靖
摘要：
为了研究曲靖烤烟钾含量特征及其与主要生态因子的关系,在曲靖烟区采集烤烟样品3 506个,测定其钾含量,并采用决策树分析、回归分析等方法探究曲靖烤烟钾含量特征及其主要影响因素。结果表明,曲靖烟区烤烟钾含量平均为1. 65%,且变幅较大( 0. 35% ~ 3. 60%) ,有81. 86%的烟叶钾含量< 2. 0%,钾含量在县域间差异达极显著水平;此外,海拔高度、土壤质地、土壤类型、土壤有机质、土壤速效钾、土壤有效钙、土壤有效镁均影响烤烟钾含量。海拔高度对烤烟钾含量影响的拐点分别为1 689、 1 814和2 023m,且随海拔上升,烤烟钾含量下降;土壤质地粘性增加,烤烟钾含量随之增加;烤烟钾含量在不同土壤类型间差异达极显著水平,其中黄壤中的含量最高( 2. 07%) ,紫色土最低( 1. 59%) ;土壤有机质对烤烟钾含量影响的拐点分别为23. 768和39. 060 g·kg ~(-1),且随土壤有机质增加,烤烟钾含量表现为逐渐增加趋势;土壤速效钾对烤烟钾含量影响的拐点分别为153. 746、205. 000和328. 169 mg·kg ~(-1),随土壤速效钾增加,烤烟钾含量表现为逐渐增加趋势;随土壤有效钙增加,烤烟钾含量呈下降趋势;随土壤有效镁增加,烤烟钾含量呈先上升高后下降的趋势,有效镁含量为300 mg·kg~(-1)左右时,烤烟钾含量达峰值;随着土壤钙钾比和镁钾比升高,烤烟钾含量呈减速下降的趋势。本研究结果对提高曲靖烟区烤烟钾含量具有一定指导意义。
语种：
中文
展开
Histone acetyltransferase inhibitors antagonize AMP-activated protein kinase in postmortem glycolysis
作者：
Li, Qiong;Li, Zhongwen;Lou, Aihua;Wang, Zhenyu;Zhang, Dequan* ;...
期刊：
ANIMAL BIOSCIENCE ,2017年30(6):857-864 ISSN：2765-0189
通讯作者：
Shen, Qingwu W.;Zhang, Dequan
作者机构：
[Shen, Qingwu W.; Lou, Aihua; Li, Qiong] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Zhang, Dequan; Li, Zhongwen; Wang, Zhenyu] Chinese Acad Agr Sci, Key Lab Agroprod Proc, Minist Agr, Inst Food Sci & Technol, Beijing 100193, Peoples R China.
通讯机构：
[Shen, Qingwu W.] H;[Zhang, Dequan] C;Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.;Chinese Acad Agr Sci, Key Lab Agroprod Proc, Minist Agr, Inst Food Sci & Technol, Beijing 100193, Peoples R China.
关键词：
AMP-activated Protein Kinase (AMPK);Pale, Soft, Exudative (PSE);Postmortem Glycolysis;Protein Acetylation
摘要：
Objective: The purpose of this study was to investigate the influence of AMP-activated protein kinase (AMPK) activation on protein acetylation and glycolysis in postmortem muscle to better understand the mechanism by which AMPK regulates postmortem glycolysis and meat quality. Methods: A total of 32 mice were randomly assigned to four groups and intraperitoneally injected with 5-Aminoimidazole-4-carboxamide1-β-D-ribofuranoside (AICAR, a specific activator of AMPK), AICAR and histone acetyltransferase inhibitor II, or AICAR, Trichostatin A (TSA, an inhibitor of histone deacetylase I and II) and Nicotinamide (NAM, an inhibitor of the Sirt family deacetylases). After mice were euthanized, the Longissimus dorsi muscle was collected at 0 h, 45 min, and 24 h postmortem. AMPK activity, protein acetylation and glycolysis in postmortem muscle were measured. Results: Activation of AMPK by AICAR significantly increased glycolysis in postmortem muscle. At the same time, it increased the total acetylated proteins in muscle 45 min postmortem. Inhibition of protein acetylation by histone acetyltransferase inhibitors reduced AMPK activation induced increase in the total acetylated proteins and glycolytic rate in muscle early postmortem, while histone deacetylase inhibitors further promoted protein acetylation and glycolysis. Several bands of proteins were detected to be differentially acetylated in muscle with different glycolytic rates. Conclusion: Protein acetylation plays an important regulatory role in postmortem glycolysis. As AMPK mediates the effects of pre-slaughter stress on postmortem glycolysis, protein acetylation is likely a mechanism by which antemortem stress influenced postmortem metabolism and meat quality though the exact mechanism is to be elucidated. Copyright © 2017 by Asian-Australasian Journal of Animal Sciences.
语种：
英文
展开
3种外源植物生长调节剂对干旱胁迫下烟草生理的影响
作者：
仲晓君;李强;周喜新;刘俊龙;李明昌;...
期刊：
安徽农业大学学报 ,2017年44(6):1139-1143 ISSN：1672-352X
作者机构：
湖南农业大学生物科技学院, 长沙, 410128;湖南农业大学烟草研究院, 长沙, 410128;湖南农业大学农学院, 长沙, 410128;云南省烟草公司曲靖市公司, 曲靖, 655000;红云红河烟草(集团)有限责任公司, 昆明, 650231
关键词：
烤烟;干旱胁迫;水杨酸;甜菜碱;氯化胆碱
摘要：
以K326品种烤烟为试验材料,采用盆栽试验研究干旱胁迫下烤烟生长与生理状况,以及喷施水杨酸、甜菜碱和氯化胆碱对烤烟抗氧化代谢影响。结果表明,干旱胁迫抑制烤烟正常生长,烟株矮小,有效叶片数目少,茎干瘦弱,叶长变短及叶宽变窄。喷施植物生长调节剂可以缓解干旱胁迫症状,喷施氯化胆碱能有效增加株高、最大叶长、有效叶片数,提高烟株干重、丙二醛(MDA)含量、脯氨酸含量及超氧化物歧化酶酶(SOD)活性;喷施甜菜碱对过氧化氢酶(CAT)活性有较好调节效应;喷施水杨酸能显著提高过氧化物酶(POD)活性,提高烤烟茎围和最大叶宽。综上所述,植物生长调节剂能提高烟株耐旱性,增强烟株对干旱环境适应性,其中以喷施氯化胆碱效果最佳。
语种：
中文
展开
曲靖烤烟镁含量特征及其影响因素
作者：
李强;周冀衡;刘晓颖;张一扬;王瑞宝;...
期刊：
土壤 ,2017年49(3):565-571 ISSN：0253-9829
作者机构：
[李强] 湖南农业大学食品科学技术学院,长沙 410128;[李强] 湖南农业大学烟草研究院,长沙 410128;湖南农业大学烟草研究院,长沙,410128;云南省烟草公司曲靖市公司,云南曲靖,655000;红云红河烟草(集团)有限责任公司,昆明,650231
关键词：
烤烟;镁含量;影响因素;曲靖
摘要：
为研究曲靖烤烟镁含量特征及其影响因素,在曲靖烟区采集烤烟样品3 506个,采用决策树分析、回归分析等方法研究了曲靖烤烟镁含量特征及其主要影响因素。结果表明:曲靖烟区烤烟镁含量平均为4.10 g/kg,且变幅较大(0.36 ~ 17.70g/kg),有46.36%面积的烟叶镁含量<4 g/kg,镁含量在县域间差异达极显著水平,海拔高度、土壤类型、质地及有机质、速效钾、有效镁、有效钙含量均影响烤烟镁含量。烤烟镁含量与海拔高度呈良好的线性关系(R~2= 0.720, P=0.000),随海拔增加,烤烟镁含量表现为下降的趋势。土壤质地黏性增加,烤烟镁含量呈下降的趋势;烤烟镁含量在不同土壤类型间差异达极显著水平,最高的是紫色土(4.98g/kg),最低的是红壤(3.82g/kg);随土壤有机质含量增加,烤烟镁含量表现为逐渐下降的趋势;随土壤有效镁和速效钾含量增加,烤烟镁含量表现为逐渐增加的趋势;随土壤有效钙含量增加,烤烟镁含量呈先升高后下降的趋势,土壤有效钙含量2 000 mg/kg左右时,烤烟镁含量达峰值;随着土壤钙镁比和钾镁比升高,烤烟镁含量呈减速下降的趋势。
语种：
中文
展开
高海拔地区光强减弱对烤烟生长发育和产量的影响
作者：
李强;仲晓君;王瑞宝;周喜新;刘加红;...
期刊：
江西农业大学学报 ,2016年38(6):1042-1048 ISSN：1000-2286
作者机构：
[李强] 湖南农业大学 食品科学技术学院，湖南 长沙 410128;湖南农业大学 烟草研究院，湖南 长沙 410128;湖南农业大学 烟草研究院,湖南 长沙,410128;云南省烟草公司曲靖市公司,云南 曲靖,655000;红云红河烟草 集团 有限责任公司,云南 昆明,650231
关键词：
烤烟;光强;生长发育;产量;遮阴
摘要：
为探讨高海拔条件下光强减弱对烤烟生长发育、产量及产量构成的影响,采用裂区设计开展大田试验,并用方差分析和多重比较的方法分析光强减弱对烤烟生长及烟叶产质量的影响。结果表明,光强减弱后烤烟生育进程推迟,烤烟叶长、叶宽和叶面积指数增加,株高和节间距增加,茎粗降低,根颈部直径、最大侧根直径降低,直径≥2 mm侧根数减少;随着光强减弱,各器官干物质积累均减少,干物质在经济器官中的分配比例下降;随着光强减弱,烟叶总产量下降,中上等烟比例下降。光强减弱对烟株生长、产量及产量构成都有显著影响。
语种：
中文
展开
Activator protein-2α functions as a master regulator of multiple transcription factors in the mouse liver
作者：
Li, Qingjie* ;Luo, Cunhui;Loehr, Christiane V.;Dashwood, Roderick H.
期刊：
Hepatology Research ,2011年41(8):776-783 ISSN：1386-6346
通讯作者：
Li, Qingjie
作者机构：
[Li, Qingjie] Univ Texas Med Branch Galveston, Div Gastroenterol, Dept Internal Med, Galveston, TX 77550 USA.;[Loehr, Christiane V.] Oregon State Univ, Coll Vet Med, Corvallis, OR 97331 USA.;[Dashwood, Roderick H.] Oregon State Univ, Linus Pauling Inst, Corvallis, OR 97331 USA.;[Dashwood, Roderick H.] Oregon State Univ, Dept Environm & Mol Toxicol, Corvallis, OR 97331 USA.;[Luo, Cunhui] Hunan Agr Univ, Coll Food Sci & Technol, Changsha, Hunan, Peoples R China.
通讯机构：
[Li, Qingjie] U;Univ Texas Med Branch Galveston, Div Gastroenterol, Dept Internal Med, Galveston, TX 77550 USA.
关键词：
activator protein 2 alpha;CCAAT binding factor;cyclic AMP responsive element binding protein;glycogen synthase kinase 3beta;hepatocyte nuclear factor 1;Myc protein;protein c Myb;protein kinase B;retinoid X receptor;Smad3 protein;Smad4 protein;transcription factor;transcription factor AP 1;transcription factor GATA 4;transcription factor PAX5;unclassified drug;upstream stimulatory factor 1;animal experiment;animal tissue;article;controlled study;DNA microarray;immunoblotting;in vivo gene transfer;liver;liver cell nucleus;male;mouse;nonhuman;priority journal;protein analysis;protein expression;protein function;protein induction;protein microarray;protein synthesis inhibition;transcription regulation;Western blotting
摘要：
Aim: Activator protein 2α (AP-2α) belongs to the AP-2 family of transcription factors that are involved in the regulation of cell proliferation, differentiation, apoptosis and carcinogenesis and has been suggested to function as a tumor suppressor in many cancers. However, the physiological role of AP-2α in hepatocytes is unknown. The present study is to characterize the expression and function of AP-2α in the liver of conscience mouse. Methods: Exogenous AP-2α was overexpressed in the mouse liver by in vivo gene delivery and changes in transcription factor expression were identified by using protein-DNA arrays and immunoblotting. Results: Western blotting and protein/DNA arrays showed that AP-2α is expressed in the nuclei of mouse hepatocytes. Overexpression of AP-2αin vivo significantly suppressed transcription factors AP-1, CREB and c-Myc, and markedly increased CBF, c-Myb, NF-1, Pax-5, RXR, Smad3/4, TR(DR-4), USF-1 and GATA. Among all GATA proteins, only GATA-4 level was dramatically elevated and there was a concomitant loss of phospho-GATA-4. Corresponding changes were detected in upstream kinases Akt, GSK-3β and PKA, which regulates the phosphorylation status and stability of GATA-4 protein. Conclusions: AP-2α is expressed in mouse hepatocytes and it acts as a master regulator of numerous transcription factors in the liver. © 2011 The Japan Society of Hepatology.
语种：
英文
展开
