期刊：
International Journal of Biological Macromolecules,2024年278(Pt 1):134606 ISSN：0141-8130
通讯作者：
Qiang Li<&wdkj&>Xingbo Shi
作者机构：
[Lu, Dai; Wu, Jiahao; Zhao, Qian; Jiang, Ziping; Xie, Xinhui; Chen, Liye] Laboratory of Micro & Nano Biosensing Technology in Food Safety, Hunan Provincial Key Laboratory of Food Science and Biotechnology, College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China;[Li, Qiang] College of Agronomy, Hunan Agricultural University, Changsha 410128, China. Electronic address: zqiangli@hunau.edu.cn;[Shi, Xingbo] Laboratory of Micro & Nano Biosensing Technology in Food Safety, Hunan Provincial Key Laboratory of Food Science and Biotechnology, College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China. Electronic address: shixingbo123@hunau.edu.cn
通讯机构：
[Qiang Li] C;[Xingbo Shi] L;College of Agronomy, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Laboratory of Micro & Nano Biosensing Technology in Food Safety, Hunan Provincial Key Laboratory of Food Science and Biotechnology, College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China
摘要：
To mitigate food spoilage caused by microbial contamination and extend the shelf life of food, antibacterial and eco-friendly biological packaging materials as an alternative to petroleum-based plastics is encouraged. Herein, an innovative and green composite film with triple antibacterial activity has been fabricated by introducing prussian blue nanoparticles (PBNPs) into chitosan (CS)-based films blended with gelatin (Gel) for the preservation of food, named CS/Gel/PB film. Due to the incorporation of PBNPs, CS/Gel/PB film exhibits enhanced mechanical, barrier and water resistance, and thermal abilities. The inherent bacterial trapping and killing capabilities of CS (contact killing), photothermal/photodynamic killing based on the excellent photothermal property of PBNPs under NIR irradiation synergistically facilitate the sterilization against Escherichia coli and Staphylococcus aureus (antibacterial ratio=99.99%). The film exhibits outstanding preservation capability in product storage, significantly extending the shelf life of strawberry and pork to 15 and 7days, respectively. Meanwhile, the cytotoxicity assessment of CS/Gel/PB against HepG2 cells ascertains a cell viability exceeding 96%, indicating a negligible toxicity level. Additionally, this film also exhibits superior biodegradability (preliminary degradation on the 10th day and completion on the 40th day) compared with PE film. Overall, these properties demonstrate great potential of CS/Gel/PB film as a novel packaging material.
摘要：
Lotus seed is well known as traditional food and medicine, but its skin is usually discarded. Recent studies have shown that lotus seed skin contains a high concentration of proanthocyanidins that have multi-functions, such as antioxidation, anti-inflammation, and anti-cancer effects. In the present study, we aimed to isolate and purify the proanthocyanidins from lotus seed skin by acetone extraction and rotary evaporation, identify their chemical structures by HPLC-MS-MS and NMR, and further investigate the antioxidant properties of the extract purified by macroporous resin (PMR) from lotus seed skin both in vitro and in vivo. The results showed that PMR mainly contained oligomeric proanthocyanidins, especially dimeric procyanidin B1 (PB1), procyanidin B2 and procyanidin B4. Although it had limited ability to directly scavenge radicals in vitro, PMR could significantly enhance the expressions of antioxidant proteins via activation of nuclear factor-E2-related factor 2 (Nrf2)-antioxidant response element (ARE) pathway in HepG2 cells. Molecular data revealed that PB1, a major component in PMR, stabilized Nrf2 by inhibiting the ubiquitination of Nrf2, which led to subsequent activation of the Nrf2-ARE pathway, including the enhancements of Nrf2 nuclear translocation, Nrf2-ARE binding and ARE transcriptional activity. Moreover, the in vivo results in high fat diet-induced mice further verified the powerful antioxidant property of PMR. These results revealed that lotus seed skin is a promising resource for functional food development.
作者机构：
[Li, Qingjie] Univ Texas Med Branch Galveston, Div Gastroenterol, Dept Internal Med, Galveston, TX 77550 USA.;[Loehr, Christiane V.] Oregon State Univ, Coll Vet Med, Corvallis, OR 97331 USA.;[Dashwood, Roderick H.] Oregon State Univ, Linus Pauling Inst, Corvallis, OR 97331 USA.;[Dashwood, Roderick H.] Oregon State Univ, Dept Environm & Mol Toxicol, Corvallis, OR 97331 USA.;[Luo, Cunhui] Hunan Agr Univ, Coll Food Sci & Technol, Changsha, Hunan, Peoples R China.
通讯机构：
[Li, Qingjie] U;Univ Texas Med Branch Galveston, Div Gastroenterol, Dept Internal Med, Galveston, TX 77550 USA.
关键词：
activator protein-1;activator protein AP-2;GATA4 transcription factor;liver;protein kinase A
摘要：
<jats:p><jats:bold>Aim: </jats:bold> Activator protein 2α (AP‐2α) belongs to the AP‐2 family of transcription factors that are involved in the regulation of cell proliferation, differentiation, apoptosis and carcinogenesis and has been suggested to function as a tumor suppressor in many cancers. However, the physiological role of AP‐2α in hepatocytes is unknown. The present study is to characterize the expression and function of AP‐2α in the liver of conscience mouse.</jats:p><jats:p><jats:bold>Methods: </jats:bold> Exogenous AP‐2α was overexpressed in the mouse liver by <jats:italic>in vivo</jats:italic> gene delivery and changes in transcription factor expression were identified by using protein‐DNA arrays and immunoblotting.</jats:p><jats:p><jats:bold>Results: </jats:bold> Western blotting and protein/DNA arrays showed that AP‐2α is expressed in the nuclei of mouse hepatocytes. Overexpression of AP‐2α<jats:italic>in vivo</jats:italic> significantly suppressed transcription factors AP‐1, CREB and c‐Myc, and markedly increased CBF, c‐Myb, NF‐1, Pax‐5, RXR, Smad3/4, TR(DR‐4), USF‐1 and GATA. Among all GATA proteins, only GATA‐4 level was dramatically elevated and there was a concomitant loss of phospho‐GATA‐4. Corresponding changes were detected in upstream kinases Akt, GSK‐3β and PKA, which regulates the phosphorylation status and stability of GATA‐4 protein.</jats:p><jats:p><jats:bold>Conclusions: </jats:bold> AP‐2α is expressed in mouse hepatocytes and it acts as a master regulator of numerous transcription factors in the liver.</jats:p>
