作者机构：
[Zeng, D. L.; Liu, W.; Wang, K. Z.; Liu, Y.] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Zhu, X. Q.] CAAS, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Key Lab Vet Parasitol Gansu Prov, Lanzhou 730046, Gansu, Peoples R China.;[Zhao, G. H.] NW A&F Univ, Coll Vet Med, Yangling 712100, Shaanxi Prov, Peoples R China.;[Tan, M. Y.] Ctr Qual Quarantine Livestock Poultry & Aquacultu, Changsha 410006, Hunan, Peoples R China.;[Zhao, G. H.; Lin, R. Q.; Zhu, X. Q.; Yuan, Z. G.] S China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China.
通讯机构：
[Zhu, X. Q.] C;CAAS, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Key Lab Vet Parasitol Gansu Prov, Lanzhou 730046, Gansu, Peoples R China.
摘要：
The prevalence of spargana infection in frogs (Rana nigromaculata) was investigated in China's central Hunan Province, from March 2007 to October 2009. 59 of 292 (20.2%) wild-caught frogs were found to be infected with plerocercoids (spargana) of the genus Spirometra. Spargana were recovered from the skeletal muscle of the hind limb. The infection rate ranged from 4.5% to 27.4%, and the infection intensity was 1-15 spargana per frog. To identify the species identity of the collected spargana, a portion of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene was amplified, sequenced, and analyzed. Sequence variations for cox1 among all the examined spargana were 0.0-3.1%, with 14 variable sites being identified in sequences obtained (3.1%, 14/446), representing 6 different cox1 sequences. Phylogenetic analysis showed that all the spargana isolates in Hunan province represented Spirometra erinaceieuropaei. This is the first report of S. erinaceieuropaei infection in frogs in Hunan province, China. (C) 2010 Elsevier B.V. All rights reserved.
作者：
Wang, G. P.;Be, Y. Y.;Hu, S. F.;Liu, W.;Liu, G. Y.*
期刊：
JOURNAL OF ANIMAL AND FEED SCIENCES,2009年18(1):61-70 ISSN：1230-1388
通讯作者：
Liu, G. Y.
作者机构：
[Hu, S. F.; Wang, G. P.; Liu, W.; Be, Y. Y.] Hunan Agr Univ, Fac Vet, Changsha 410128, Hunan, Peoples R China.;[Liu, G. Y.] Yunnan Agr Univ, Kell Lab Anim Nutr & Feed Yunnan Prov, Kunming 650201, Peoples R China.
通讯机构：
[Liu, G. Y.] Y;Yunnan Agr Univ, Kell Lab Anim Nutr & Feed Yunnan Prov, Kunming 650201, Peoples R China.
摘要：
The complete coding sequences of three porcine genes - NDP, CLN3 and NCF1 - were amplified using the reverse transcriptase polymerase chain reaction (RT-PCR) based on the conserved sequence information of the human, mouse, and referenced highly homologues pig ESTs. The sequence analyses of these three genes revealed that porcine NDP gene encodes a protein of 133 amino acids which has high homology with the Norrie disease protein homolog (NDP) of four species: bovine (98%) human (96%), crab-eating macaque (96%) and mouse (95%). The porcine CLN3 gene encodes a protein of 438 amino acids which has high homology with the ceroid-lipofuscinosis, neuronal 3 (CLN3) of four species: human (90%), crab-eating macaque (90%), dog (89%) and mouse (83%). The porcine NCF1 gene encodes a protein of 392 amino acids that has high homology with the neutrophil cytosolic factor 1(NCF1) of three species: bovine (87%), human (82%) and mouse (79%). Phylogenetic tree analysis revealed that the porcine NDP and NCF1 has closer genetic relationships with the bovine NDP and NCF1, but the porcine CLN3 has a closer genetic relationship with the CLN3 of human and crab-eating macaque. The tissue transcription profile analyses indicated that pig CLN3 and NCF1 genes were generally expressed in most of tissues, pig NDP gene was expressed in muscle, spleen, brain, lung but not expressed in kidney, liver, backfat and pancreas. These data serve as a foundation for further research on these three genes which have been associated with human disease.
