作者机构：
[Tan, L.; Wang, T.; Liu, Y.; Liu, YS; Liu, W.; Liu, Y. S.] Hunan Agr Univ, Coll Vet Med, Changsha, Hunan, Peoples R China.;[Wang, T.] Changsha Best Biol Technol Inst, Changsha, Hunan, Peoples R China.;[Liu, X. S.] Hunan Biol & Electromech Acad, Changsha, Hunan, Peoples R China.;[Liu, Y. S.] Hunan Agr Univ, Hunan Key Lab Tradit Chinese Vet Med, Changsha, Hunan, Peoples R China.
通讯机构：
[Liu, Y; Liu, YS; Liu, Y. S.] H;Hunan Agr Univ, Coll Vet Med, Changsha, Hunan, Peoples R China.;Hunan Agr Univ, Hunan Key Lab Tradit Chinese Vet Med, Changsha, Hunan, Peoples R China.
摘要：
Parasite classification and identification are central to controlling parasitosis. Traditional methods for identifying parasite species are based on morphological features, but these are time-consuming and inaccurate, especially for cryptic species. The purpose of the present study was to select molecular markers to promote the development of molecular systematic for parasites. The internal transcribed spacers (ITS) of nuclear ribosomal DNA (rDNA) falls in between 18S, 5.8S, and 28S rDNA sequences, including ITS-1 and ITS-2 sequences. Previous studies have demonstrated that rDNA ITS sequences provide useful genetic markers for identifying parasitic nematodes. With the ultimate goal of controlling parasite transmission, we identified Kalicephalus belonging to three species using ITS rDNA genes. The ITS genes (750-797 bp) of 21 Kalicephalus belonging to 3 species were cloned and sequenced. Intra- and interspecific identities were 98.4% and 80%-89%, respectively. The phylogenetic tree reconstructed with the neighbour-joining (NJ) method revealed that congener Kalicephalus form the same branch, which is far apart from other branches of other nematodes. This is consistent with morphological classifications, demonstrating the accuracy of our molecular method. This is the first report stating that ITS genes can be used to classify Kalicephalus, and it lays the foundation for identification, molecular epidemiology, and phylogenetics of Kalicephalus and related parasitic nematodes.
摘要：
T-2 and HT-2 toxins can cause cytotoxicity and oxidative stress in animals, while DL-Selenomethionine plays an important role in preventing oxidative stress and improving cell viability. However, the role of DL-Selenomethionine in T-2/HT-2 toxins-induced cell damage is still unknown. In this study, we investigated whether DL-Selenomethionine plays a protective role against T-2/HT-2-induced cytotoxicity and oxidative stress in primary hepatocytes. Our results demonstrated that T-2/HT-2 toxins-exposed broiler hepatocytes exhibited significantly decreased cell viability and intracellular glutathione (GSH) concentration while increased Lacate dehydrogenase (LDH) leakage, intracellular reactive oxygen species (ROS), glutathione peroxidase (GSH-PX), malondialdehyde (MDA) and catalase (CAT) levels, as well as elevated expression levels of genes related to oxidative stress, in a toxin dose-dependent manner (P < 0.05). However, the application of DL-Selenomethionine into T-2/HT-2 treated hepatocytes effectively alleviated the adverse effects of T-2/HT-2, as demonstrated by increased cell viability, decreased LDH leakage, declined intracellular ROS and MDA levels, increased expression of oxidative stress-related genes, as well as accordingly enhanced activities of GSH, GSH-PX, SOD and CAT as compared to the control groups (P < 0.05). Therefore, our in vitro data demonstrate that DL-Selenomethionine can function as an effectively protective agent against T-2/HT-2-induced cytotoxicity and oxidative stress.
作者机构：
[Zhou, Yan; Luo, Ziqiang; Zhao, Feiyan; Cheng, Haipeng] Cent South Univ, Dept Physiol, Xiangya Sch Med, Changsha, Hunan, Peoples R China.;[Yang, Lei; Zhao, Feiyan] Hunan Agr Univ, Coll Vet Med, Changsha, Hunan, Peoples R China.;[Liu, Wei; Hua, Qingzhong; Tang, Siyuan] Cent South Univ, Xiangya Nursing Sch, Changsha, Hunan, Peoples R China.;[Yue, Shaojie] Cent South Univ, Xiangya Hosp, Dept Pediat, Changsha, Hunan, Peoples R China.
通讯机构：
[Luo, Ziqiang; Tang, Siyuan] C;Cent South Univ, Dept Physiol, Xiangya Sch Med, Changsha, Hunan, Peoples R China.;Cent South Univ, Xiangya Nursing Sch, Changsha, Hunan, Peoples R China.
摘要：
Granulocyte colony-stimulating factor (G-CSF) can promote the repair of a variety of damaged tissues, but the underlying mechanisms have not yet been fully elucidated. Bone marrow mesenchymal stem cells (BM-MSCs) play an important role in the repair of damaged tissue. The aim of this study was to explore whether pretreating BM-MSCs with G-CSF can promote their ability of homing to the lung after in vitro transplantation via upregulating the CXCR4 expression, potentially markedly increasing the antifibrotic effect of BM-MSCs. The BM-MSCs pretreated with G-CSF were transplanted into a mouse on day 14 after bleomycin injection. The antifibrotic effects of BM-MSCs in mice were tested on day 21 by using pathological examination and collagen content assay. Pretreatment of BM-MSCs with G-CSF significantly promoted their ability of homing to the lung and enhanced their antifibrotic effects. However, knocking down the CXCR4 expression in BM-MSCs significantly inhibited the ability of G-CSF to promote the migration and homing of BM-MSCs to the lung and the resulting antifibrotic effects. We also found that G-CSF significantly increased the CXCR4 expression and AKT phosphorylation in BM-MSCs, and the AKT pathway inhibitor LY294002 significantly diminished the ability of G-CSF to upregulate the CXCR4 expression in BM-MSCs. Pretreatment of BM-MSCs with G-CSF promotes the homing of BM-MSCs to the lung via upregulating the CXCR4 expression, leading to a marked increase in the antifibrotic effects of BM-MSCs. This study provides new avenues for the application of BM-MSCs in the repair of different tissues.
作者机构：
[L.Tan,; A.B.Wang,; J.Yi; J.Y.Li; W.Liu,,] College of Veterinary Medicine,Hunan Agricultural University;[L.Tan,; A.B.Wang,; J.Yi; J.Y.Li; W.Liu,,] The Key Laboratory of Animal Vaccine & Protein Engineering;[L.Tan,; A.B.Wang,; J.Yi; J.Y.Li; W.Liu,,] College of Veterinary Medicine,South China Agricultural University
会议名称：
第十一届全国寄生虫学青年工作者学术研讨会
会议时间：
2018-08-10
会议地点：
中国云南昆明
会议论文集名称：
第十一届全国寄生虫学青年工作者学术研讨会摘要集
关键词：
Trichuris suis;infection;pigs;internal transcribed spacer（ITS）;Hunan province
摘要：
objective:The objective of this research was to investigate the prevalence of T.suis in pigs in Hunan province.methods:Total 2267 fresh fecal samples distributed in 28 pig farms from 7 different administrative regions（Hunan province） were evaluated for the existence of T.suis eggs using saturated Na Cl floating method,the internal transcribed spacer（ITS） regions from nuclear ribosomal DNA（r DNA） of 7 T.suis isolates were cloned and analyzed to determine genetic variation of the gained T.suis isolates in the present study.results:The average infection rate of T.suis in pigs was 8.91% in Hunan province;nucleotide diversities were 1.03.5% and 03.8% for ITS-1 and ITS-2,respectively.Phylogenetic analyses indicated that the collected T.suis isolates were available in the Genbank and clustered in three haplogroups.conclusion:The present investigation revealed high infection rates of T.suis in pigs in Hunan province,which shed light on making effective measures to prevent and control T.suis infection in pigs in Hunan province.
关键词：
Genetic variation;mitochondrial DNA (mtDNA);internal transcribed spacer (ITS);phylogenetic analysis;Taenia multiceps;China
摘要：
Taenia multiceps, one of the most widely distributed zoonotic tapeworm parasites, is able to parasitize the small intestine of canids. The metacestode of T.multiceps is fatal to ruminants and causes important economic losses in livestock. However, molecular characteristics of T.multiceps and coenurus in China are still unclear. In this study, 36 goat isolates of the coenurus stage and 18 dog isolates of the adult stage of T.multiceps were obtained from three geographical areas in China and the isolated parasite above were analyzed by amplifying the partial of cytochrome coxidase subunit 1(pcox1), 12S ribosomal RNA (12S rRNA) from mitochondrial DNA (mtDNA) regions and an internal transcribed spacer (ITS) of ribosomal DNA (rDNA). These DNA sequences obtained from T.multiceps and coenurus were employed to evaluate the nucleotide diversity and confirm the relationship between T.multiceps and coenurus. Sequences variation were 0–1.4%, 0–1.5%, 0–4.2% for pcox1, 12S rRNA and ITS, respectively, among T.multiceps and coenurus isolates obtained in this study. In Sichuan province, sequence variations for Coenurus cerebralis isolated from Yaan city were 0–1.4% for pcox1, 0–1.0% for 12S rRNA and 0–2.1% for ITS. In Hunan province, variations were 0–1.0%, 0–1.5% and 0–3.3% for corresponding genes for non-coenurus cerebralis isolated from Changsha city, while variations of T.multiceps isolates from Xiangxi autonomous prefecture were 0–1.0%, 0–1.1% and 0–3.4% for pcox1, 12S rRNA and ITS, respectively. Phylogenetic analysis based on pcox1 sequences indicated that all cerebral and noncerebral metacestodes belong to T.multiceps. These results provide reference values for future molecular epidemiological and biological study on T.multiceps in dogs and intermediate hosts.
摘要：
Targeted integration of exogenous genes into so-called safe harbors/friend sites, offers the advantages of expressing normal levels of target genes and preventing potentially adverse effects on endogenous genes. However, the ideal genomic loci for this purpose remain limited. Additionally, due to the inherent and unresolved issues with the current genome editing tools, traditional embryonic stem (ES) cell-based targeted transgenesis technology is still preferred in practical applications. Here, we report that a high and repeatable homologous recombination (HR) frequency (>95%) is achieved when an approximate 6kb DNA sequence flanking the MYH9 gene exon 2 site is used to create the homology arms for the knockout/knock-in of diverse nonmuscle myosin II (NM II) isoforms in mouse ES cells. The easily obtained ES clones greatly facilitated the generation of multiple NM II genetic replacement mouse models, as characterized previously. Further investigation demonstrated that though the targeted integration site for exogenous genes is shifted to MYH9 intron 2 (about 500bp downstream exon 2), the high HR efficiency and the endogenous MYH9 gene integrity are not only preserved, but the expected expression of the inserted gene(s) is observed in a pre-designed set of experiments conducted in mouse ES cells. Importantly, we confirmed that the expression and normal function of the endogenous MYH9 gene is not affected by the insertion of the exogenous gene in these cases. Therefore, these findings suggest that like the commonly used ROSA26 site, the MYH9 gene locus may be considered a new safe harbor for high-efficiency targeted transgenesis and for biomedical applications.
期刊：
Korean Journal of Parasitology,2018年56(5):495-500 ISSN：0023-4001
通讯作者：
Liu, Wei;Li, Jiayu
作者机构：
[Liu, Yisong; Wang, Aibing; Liu, Wei; Tan, Lei; Yi, Jing] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Wang, Aibing; Liu, Wei; Tan, Lei] Hunan Agr Univ HUNAU, Coll Vet Med, Key Lab Anim Vaccine & Prot Engn, LAMFG, Changsha 410128, Hunan, Peoples R China.;[Wang, Aibing; Liu, Wei; Tan, Lei] R&D Ctr Anim Reverse Vaccinol Hunan Prov, Changsha, Hunan, Peoples R China.;[Li, Jiayu] South China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China.
通讯机构：
[Liu, Wei] H;[Liu, Wei] R;[Li, Jiayu] S;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ HUNAU, Coll Vet Med, Key Lab Anim Vaccine & Prot Engn, LAMFG, Changsha 410128, Hunan, Peoples R China.
关键词：
Trichuris suis;prevalence;pig;internal transcribed spacer (ITS);Hunan province
摘要：
Trichuris suis infection in pigs is ubiquitous in intensive and extensive farms, which causes potential threat to human health. The objective of this research was to investigate the prevalence of T. suis in pigs in Hunan province. Total 2,267 fresh fecal samples distributed in 28 pig farms from 7 different administrative regions (Hunan province) were evaluated for the existence of T. suis eggs using saturated NaCl floating method. The average infection rate of T. suis in pigs was 8.91% in Hunan province. To determine genetic variation of the gained T. suis isolates in the present study, the internal transcribed spacer (ITS) regions from nuclear ribosomal DNA (rDNA) of 7 T. suis isolates were cloned and analyzed. Nucleotide diversities were 1.0-3.5% and 0-3.8% for ITS-1 and ITS-2, respectively. Phylogenetic analyses indicated that all isolates collected in the present study and T. suis available in Genbank generated a monophyletic clade. The present investigation revealed high infection rates of T. suis in pigs in Hunan province, which shed light on making effective measures to prevent and control T. suis infection in pigs in Hunan province.
