作者： Ma, Wenyue;Zhang, Ya*;Wang, Chong;Liu, Shuangqing;Liao, Xiaolan*

期刊： PLANT PROTECTION SCIENCE,2018年54(2):92-100 ISSN：1212-2580

通讯作者： Zhang, Ya;Liao, Xiaolan

作者机构： [Zhang, Ya; Liu, Shuangqing; Ma, Wenyue; Zhang, Y; Liao, XL; Liao, Xiaolan] Hunan Agr Univ, Coll Plant Protect, Dept Plant Protect, Changsha, Hunan, Peoples R China.;[Wang, Chong] Hunan Agr Univ, Sci Coll, Dept Chem, Changsha, Hunan, Peoples R China.;[Liao, Xiaolan] Hunan Prov Key Lab Biol & Control Plant Dis & Pla, Changsha, Hunan, Peoples R China.

通讯机构： [Zhang, Y; Liao, XL; Liao, Xiaolan] H;Hunan Agr Univ, Coll Plant Protect, Dept Plant Protect, Changsha, Hunan, Peoples R China.;Hunan Prov Key Lab Biol & Control Plant Dis & Pla, Changsha, Hunan, Peoples R China.

关键词： Fragaria ananassa Duch.;new pathogenic fungi;Geotrichum candidum;identification;rDNA-ITS

摘要： A new disease of strawberry (Fragaria ananassa Duch.) was discovered in the Lianqiao strawberry planting base in Shaodong County, in Hunan Province, China. In the early disease stage, leaves showed small black spots surrounded by yellow halos, while in the late stage, a white fluffy layer of mold appeared. Fruits were covered with a white layer of mold. The symptoms were observed using in vitro inoculation experiments. After the spray-inoculation of stabbed leaves, small black spots surrounded by yellow halos occurred on leaves, with no clear boundary between diseased and healthy areas. In the late stage, disease spots gradually expanded and a white fluffy layer of mold formed under humid conditions. Unstabbed leaves had almost no disease occurrence after spray-inoculation. After the spray-inoculation of stabbed fruits, by the late stage, a dense white layer of mold formed. According to Koch’s postulates, the isolated strain was verified as a pathogen. The pathogenic strain, designated SDLQ16, was isolated from diseased fruit by dilution method and tentatively identified as G. candidum based on the culture characteristics, morphologies, physio-biochemical analysis, and phylogenetic analysis of the rDNA-ITS sequence. The fungus was able to grow on different culture mediums, with a broad range of nutrition. The colonies on PDA medium were raised and pale white, with a neat edge and visible hyphae. The hyphae were friable but the spores were developing. Basal hyphae rapidly grew close to the medium to 3.2–4.2 µm in diameter, with septa and forked branches at acute angles. The solitary or beaded spores with smooth surfaces were 3.5–7.5 µm in length and 3.5–4.5 µm in width. This strain was able to gelatin liquefaction, proteolysis, grease, peptonised milk, urea, and so on. The pathogenicity on strawberry from strong to weak was: fruit > leaf > stem. A BLAST algorithm was used to query SDLQ16’s rDNA-ITS sequence (cloned and deposited as GenBank number KU373122) against the NCBI database, and it was located in the Acinetobacter sp. branch of a phylogenetic tree. SDLQ16 was most closely related to Geotrichum candidum ATCC34614 (GQ4580314.1), with a sequence similarity of 99%. © 2018, Czech Academy of Agricultural Sciences. All rights reserved.

语种： 英文

作者： Zhang, Ya;Liu, Shuangqing*;Wang, Chong;Liao, Xiaolan*

期刊： International Journal of Agriculture and Biology,2018年20(11):2378-2386 ISSN：1560-8530

通讯作者： Liu, Shuangqing;Liao, Xiaolan

作者机构： [Zhang, Ya; Liu, Shuangqing; Liao, Xiaolan; Liao, XL] Hunan Agr Univ, Coll Plant Protect, Changsha, Hunan, Peoples R China.;[Liu, Shuangqing] Hunan Prov Key Lab Biol & Control Plant Dis & Pla, Changsha, Hunan, Peoples R China.;[Wang, Chong] Hunan Agr Univ, Sci Coll, Changsha, Hunan, Peoples R China.;[Liao, Xiaolan] Hunan Biol Pesticides & Pesticide Preparat Proc E, Changsha, Hunan, Peoples R China.

通讯机构： [Liu, SQ; Liao, XL; Liu, Shuangqing; Liao, Xiaolan] H;Hunan Agr Univ, Coll Plant Protect, Changsha, Hunan, Peoples R China.;Hunan Prov Key Lab Biol & Control Plant Dis & Pla, Changsha, Hunan, Peoples R China.;Hunan Biol Pesticides & Pesticide Preparat Proc E, Changsha, Hunan, Peoples R China.

关键词： Carboxamidebiological characteristics;Derivative;Fungicidal activity;Phenazine-1-carboxamide;Plant pathogenic fungus;Rhizoctonia solani

摘要： A novel phenazine-l-carboxamide-derived 18-1 (PCND 18-1) was evaluated in terms of its potential for the development and enrichment of new biofungicides for the control of rice sheath blight caused by Rhizoctonia solani. PCND 18-1 exhibited fungicidal activity against R. solani, showing an inhibition rate of 87.64%, with a 50% effective concentration (EC50) 4.25 mu g/mL, regression equation of Y = 0.7105x + 3.8428; and correlation coefficient of 0.9817, which are indicative of its potential as a natural biofungicide. PCND 18-1 also attenuated the pathogenicity of R. solani, which was concentration-dependent. Additionally, the action mode of PCND 18-1 against rice sheath blight (R. solani) was protective better than curative activity under greenhouse condition, as reflected by the corresponding EC50 values of 2.49 and 5.72 mu g/mL. PCND 18-1 can translocate in rice with a low translocation capacity and exhibited a higher capacity for upward (root-leaf) translocation than for downward (leaf-root) translocation. Furthermore, PCND 18-1 demonstrated adhesion to leaves but poor tolerance against rain-washing. The optimal persistence period of PCND 18-1 on rice was 7 d. (C) 2018 Friends Science Publishers

语种： 英文

作者： Xiang, Yaqin;Zhang, Ya*;Wang, Chong;Liu, Shuangqing;Liao, Xiaolan*

期刊： Pesticide Biochemistry and Physiology,2018年147:32-39 ISSN：0048-3575

通讯作者： Zhang, Ya;Liao, Xiaolan

作者机构： [Zhang, Ya; Liu, Shuangqing; Xiang, Yaqin; Zhang, Y; Liao, XL; Liao, Xiaolan] Hunan Agr Univ, Dept Plant Protect, Coll Plant Protect, Changsha, Hunan, Peoples R China.;[Wang, Chong] Hunan Agr Univ, Dept Chem, Sci Coll, Changsha, Hunan, Peoples R China.;[Liao, Xiaolan] Hunan Prov Key Lab Biol & Control Plant Dis & Pla, Changsha, Hunan, Peoples R China.

通讯机构： [Zhang, Y; Liao, XL] H;Hunan Agr Univ, Dept Plant Protect, Coll Plant Protect, Changsha, Hunan, Peoples R China.

关键词： antifungal agent;chitin synthase;phenazine derivative;phenazine-1-carboxamide;reduced nicotinamide adenine dinucleotide dehydrogenase (ubiquinone);antagonists and inhibitors;cell wall;crop;drug effect;enzymology;fungal gene;genetics;growth, development and aging;microbiology;mitochondrion;mycelium;plant disease;plant root;prevention and control;real time polymerase chain reaction;Rhizoctonia;scanning electron microscopy;transmission electron microscopy;ultrastructure;Antifungal Agents;Cell Wall;Chitin Synthase;Crops, Agricultural;Electron Transport Complex I;Genes, Fungal;Microscopy, Electron, Scanning;Microscopy, Electron, Transmission;Mitochondria;Mycelium;Phenazines;Plant Diseases;Plant Roots;Real-Time Polymerase Chain Reaction;Rhizoctonia

摘要： The purpose of this research was to explore the effect of phenazine-1-carboxamide (PCN) on Rhizoctonia solani and to elucidate its mechanisms of action. The toxicity of PCN to R. solani was measured using a growth rate method. The results indicated that PCN inhibited R. solani with a 50% effective concentration (EC50) of 9.0934 μg/mL. The mycelia of R. solani were then exposed to 18.18 μg/mL (2EC50) of PCN. Optical microscopy, scanning electron microscopy (SEM), and transmission electron microscopy (TEM) were used to observe the effects of PCN on mycelial morphology and ultrastructure. Following the PCN treatment, the optical microscopy observations revealed that the mycelia appeared twisted; the branching mycelia grew, but the main mycelia did not grow following branching; and the mycelial roots possessed more vacuoles. SEM observations revealed that the mycelia were locally swollen and exhibited a sharp decrease in prominence. TEM observations showed that the cell wall became thin and deformed; the mitochondria disappeared; the septum twisted; and most of the organelles were difficult to discern. Conversely, all of the organelles could be clearly observed in the control. We then used real-time quantitative PCR and an enzyme activity testing kit to further explore the effects of PCN on the cell wall and mitochondria. Physiological and biochemical results demonstrated that both the cell wall and mitochondria constitute are PCN targets. PCN inhibited the activities of chitin synthetase and complex I of the mitochondria electron transport chain. Molecular experiments demonstrated that PCN controlled the growth of R. solani mycelia by inhibiting the expression level of chitin synthetase genes. Future research on PCN should investigate its influence on metabolic pathways, thereby aiding in the potential development of novel pesticides. © 2017 Elsevier Inc.

语种： 英文

作者： Zhu, Xiaolu;Wang, Canglong*;Liu, Jiajia;Zhang, Xingming;Deng, Huiqiu;...

期刊： RSC Advances,2018年8(62):35735-35743 ISSN：2046-2069

通讯作者： Duan, Wenshan;Wang, Canglong

作者机构： [Duan, Wenshan; Zhu, Xiaolu; Liu, Jiajia] Northwest Normal Univ, Coll Phys & Elect Engn, Lanzhou 730070, Gansu, Peoples R China.;[Wang, Canglong; Yang, Lei; Zhu, Xiaolu; Liu, Jiajia] Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R China.;[Zhang, Xingming] Hunan Agr Univ, Coll Sci, Changsha 410128, Hunan, Peoples R China.;[Deng, Huiqiu] Hunan Univ, Sch Phys & Elect, Dept Appl Phys, Changsha 410082, Hunan, Peoples R China.

通讯机构： [Duan, Wenshan] N;[Wang, Canglong] C;Northwest Normal Univ, Coll Phys & Elect Engn, Lanzhou 730070, Gansu, Peoples R China.;Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R China.

摘要： The beryllide Be12Ti is considered to be the most promising candidate material for advanced plasma facing materials in future fusion reactors because of its excellent performance. In this work, first-principles calculations were conducted to gain insight into the retention and diffusion behavior of transmutation H and He atoms in Be12Ti. The solution energy and migration energy of single impurity H/He atoms were computed to study the behavior of their retention and diffusion. Among seven stable interstitial sites, H atoms preferentially occupy the octahedral interstitial site, Ioct, whereas He atoms preferentially occupy the dodecahedral interstitial site, Idode. The solubility of H is much higher than that of He in Be12Ti. When monovacancy is generated, H atoms preferentially stay in the vicinity of Be1 vacancies, while He atoms tend to reside in the center of Ti vacancies. The migration energy barrier of a single He atom between first near-neighbor Idode sites is 0.35 eV. For H atoms, the migration energy barrier from Idode to Itetra2 is 0.45 eV. The barrier along the paths Itri1-Idode-Itri1 is 0.38 eV. When a Be3 vacancy is introduced as the neighbour of Itri1, the migration energy barrier increases to 0.77 eV. These results indicate that vacancies can trap impurity atoms and may act as seeds for bubble formation. © The Royal Society of Chemistry.

语种： 英文

作者： Xing, Wei;Li, Jinlong;Li, Peng;Wang, Chong;Cao, Yanan;...

期刊： 环境科学学报(英文版),2018年65(03):262-270 ISSN：1001-0742

通讯作者： Zuo, Jiane

作者机构： [Li, Jinlong; Xing, Wei; Li, Desheng] Beijing Jiaotong Univ, Sch Civil Engn, Beijing 100044, Peoples R China.;[Yang, Yunfeng; Zhou, Jizhong; Cao, Yanan; Wang, Chong; Li, Peng; Zuo, Jiane] Tsinghua Univ, Sch Environm, State Key Joint Lab Environm Simulat & Pollut Con, Beijing 100084, Peoples R China.;[Xing, Wei; Li, Desheng] Beijing Key Lab Aqueous Typ Pollutants Control &, Beijing 100044, Peoples R China.;[Wang, Chong] Hunan Agr Univ, Coll Sci, Changsha 410128, Hunan, Peoples R China.;[Zhou, Jizhong] Univ Oklahoma, Inst Environm Genom, Norman, OK 73019 USA.

通讯机构： [Zuo, Jiane] T;Tsinghua Univ, Sch Environm, State Key Joint Lab Environm Simulat & Pollut Con, Beijing 100084, Peoples R China.

关键词： Carbon;Denitrification;Effluent treatment;Effluents;Microorganisms;Nitrogen;RNA;Throughput;Wastewater treatment;Water treatment;Biodegradable organics;Heterotrophic denitrification;High-throughput sequencing;Hydrogenophaga;Hydrogenotrophic denitrification;Municipal wastewater treatment plants;Organics;Removal from municipal wastewaters;Nitrogen removal;autotrophy;denitrification;DNA;effluent;microbial community;RNA;wastewater;Hydrogenophaga;Thauera;Thermomonas;chemistry;denitrification;drug effects;microbiology;procedures;sewage;toxicity;waste water;water pollutant;Denitrification;Waste Disposal, Fluid;Waste Water;Water Microbiology;Water Pollutants

摘要： Hydrogenotrophic denitrification is promising for tertiary nitrogen removal from municipal wastewater. To reveal the influence of residual organics in municipal wastewater on hydrogenotrophic denitrifiers, we adopted high-throughput 16S rRNA gene amplicon sequencing to examine microbial communities in hydrogenotrophic denitrification enrichments. Using effluent from a municipal wastewater treatment plant as water source, COD, nitrate and pH were controlled the same except for a gradient of biodegradable carbon (i.e., primary effluent (PE), secondary effluent (SE), or combined primary and secondary effluent (CE)). Inorganic synthetic water (IW) was used as a control. Hydrogenophaga, a major facultative autotroph, accounted for 17.1%, 5.3%, 32.7% and 12.9% of the sequences in PE, CE, SE and IW, respectively, implicating that Hydrogenophaga grew well with or without organics. Thauera, which contains likely obligate autotrophic denitrifiers, appeared to be the most dominant genera (23.6%) in IW and accounted for 2.5%, 4.6% and 8.9% in PE, CE and SE, respectively. Thermomonas, which is related to heterotrophic denitrification, accounted for 4.2% and 7.9% in PE and CE fed with a higher content of labile organics, respectively. In contrast, Thermomonas was not detected in IW and accounted for only 0.6% in SE. Our results suggest that Thermomonas are more competitive than Thauera in hydrogenotrophic denitrification with biodegradable organics. Moreover, facultative autotrophic denitrifiers, Hydrogenophaga, are accommodating to residual organic in effluent wastewater, thus we propose that hydrogenotrophic denitrification is amenable for tertiary nitrogen removal.

语种： 英文

作者： 刘妍;张亚;项雅琴;王翀;廖晓兰

期刊： 植物保护,2018年44(2):235-240 ISSN：0529-1542

作者机构： [刘妍; 张亚; 项雅琴; 廖晓兰] 湖南农业大学植物保护学院;[刘妍] 植物病虫害生物学与防控湖南省重点实验室;[张亚] 湖南省生物农药与农药制剂加工工程技术研究中心;[王翀] 湖南农业大学理学院

关键词： 啶酰菌胺;氟啶胺;水稻纹枯病菌;草莓灰霉病菌;复配物;增效作用

摘要： 为探明啶酰菌胺与氟啶胺复配物对水稻纹枯病菌和草莓灰霉病菌的增效潜力,采用菌丝生长速率法测定啶酰菌胺和氟啶胺复配物对水稻纹枯病菌和草莓灰霉病菌的抑制效果,并根据Wadley方法评价复配物增效作用。结果表明:啶酰菌胺和氟啶胺对水稻纹枯病菌均具有抑制作用,其EC_(50)分别为0.483 6 μg/mL和0.054 1 μg/mL。将啶酰菌胺和氟啶胺按照1∶5、1∶4、1∶3、1∶2、1∶1、2∶3、3∶2、5∶1、4∶1、3∶1和2∶1的比例进行复配,结果表明:按照1∶2、3∶2、5∶1、4∶1、3∶1和2∶1复配对水稻纹枯病菌均具有增效作用,其中以3∶1和3∶2比例复配增效最佳,增效系数分别为7.7和5.46;以1∶1、1∶3、1∶4、1∶5和2∶3复配对水稻纹枯病菌具有相加作用。啶酰菌胺和氟啶胺对草莓灰霉病菌也具有抑制作用,其EC_(50)分别为1.637 1 μg/mL和0.028 3 μg/mL。将啶酰菌胺和氟啶胺按照上述相同11种比例进行复配,结果显示比例为1∶5、1∶4、1∶1、5∶1、4∶1等8种复配物对草莓灰霉病菌具有相加作用,其中以2∶1和1∶2比例复配相加效果最佳,增效系数分别为1.30和1.00,但比例为3∶2等其他比例复配则对草莓灰霉病菌表现出拮抗作用。

语种： 中文

作者： Liu, Jiajia;Wang, Canglong*;Zhu, Xiaolu;Liu, Jitao;Zhang, Xingming;...

期刊： PHYSICAL CHEMISTRY CHEMICAL PHYSICS,2018年20(27):18766-18774 ISSN：1463-9076

通讯作者： Duan, Wenshan;Wang, Canglong

作者机构： [Duan, Wenshan; Zhu, Xiaolu; Liu, Jiajia; Gou, Xueqiang] NorthWest Normal Univ, Coll Phys & Elect Engn, Lanzhou 730000, Gansu, Peoples R China.;[Wang, Canglong; Yang, Lei; Liu, Jitao] Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R China.;[Zhang, Xingming] Hunan Agr Univ, Coll Sci, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Duan, Wenshan] N;[Wang, Canglong] C;NorthWest Normal Univ, Coll Phys & Elect Engn, Lanzhou 730000, Gansu, Peoples R China.;Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R China.

摘要： First principles calculations have been performed to investigate the synergetic effect of H and He impurities with vacancies in Ti 3 AlC 2 . The configurations and energetics of H n -He-V Al complexes (n ≤ 4) and He-He/He-H/H-H interactions have been studied. It is found that the impurity H atom prefers to occupy the tetrahedral interstitial site (I tetr -3), but the He atom prefers to occupy the octahedral interstitial site (I oct -4) in perfect Ti 3 AlC 2 . Within a pre-existing Al vacancy, the most favorable site for a He atom is close to tetr-site, meanwhile the H atom preferentially deviates from the vacancy center with the separation 1.3 Å along the 〈001〉 direction. He-H and He-He show a weakly attractive interaction, but weak repulsion occurs in the H-H interaction, which is different from the case of Ti 3 SiC 2 . The He-V Al complex plays an important role in the trapping of H atoms. The He-V Al cluster can trap up to three H atoms in the absence of H 2 molecules, which leads to the formation of a H-He hybridized bubble. Thus, the He atom can subsequently suppress further aggregation of H atoms and block hydrogen embrittlement and volume swelling. © the Owner Societies.

语种： 英文

作者： Ji, Yongsheng;Fang, Dong*;Wang, Chang;Zhou, Zhi;Luo, Zhiping;...

期刊： Journal of Alloys and Compounds,2018年742:567-576 ISSN：0925-8388

通讯作者： Fang, Dong;Huang, Jing;Yi, Jianhong

作者机构： [Fang, Dong; Wang, Chang; Huang, Jing; Fang, D; Huang, J; Ji, Yongsheng] Wuhan Text Univ, Key Lab Green Proc & Funct Text New Text Mat, Wuhan 430200, Hubei, Peoples R China.;[Zhou, Zhi] Hunan Agr Univ, Coll Sci, Changsha 410128, Hunan, Peoples R China.;[Luo, Zhiping] Fayetteville State Univ, Dept Chem & Phys, Fayetteville, NC 28301 USA.;[Fang, Dong; Yi, Jianhong] Kunming Univ Sci & Technol, Fac Mat Sci & Engn, Kunming 650093, Yunnan, Peoples R China.

通讯机构： [Fang, D; Huang, J] W;[Yi, Jianhong] K;Wuhan Text Univ, Key Lab Green Proc & Funct Text New Text Mat, Wuhan 430200, Hubei, Peoples R China.;Kunming Univ Sci & Technol, Fac Mat Sci & Engn, Kunming 650093, Yunnan, Peoples R China.

关键词： Cobalt;Cobalt deposits;Cyclic voltammetry;Electron microscopy;Fourier transform infrared spectroscopy;High resolution transmission electron microscopy;Hydrothermal synthesis;Lithium;Lithium-ion batteries;Nanowires;Polyacrylonitriles;Scanning electron microscopy;Storage (materials);Transmission electron microscopy;Vanadium compounds;X ray diffraction;Ammonium vanadate;Charge discharge tests;Electrochemical lithium storages;Hydrothermal methods;Initial discharge capacities;Lithium ion storages;Lithium storage properties;Nanowire arrays;X ray photoelectron spectroscopy

摘要： Cobalt (Co)-doped V2O5 nanowire arrays are prepared using a simple hydrothermal method by controlling appropriate molar ratios of cobaltous acetate (Co(CH3COO)2·4H2O) and ammonium vanadate (NH4VO3). The products are characterized by scanning electron microscopy, X-ray diffraction, transmission electron microscopy and Fourier-transform infrared spectroscopy. The nanowires are identified as cobalt-doped V2O5 when the molar ratio of cobalt/vanadium precursors is less than 0.064 (calculated from raw materials); while as the molar ratio increases to 0.128, the nanowires are composed of a mixture of V2O5 and Co3O4. The valence states of Co, O and V are tested by X-ray photoelectron spectroscopy, and the distribution of these elements is confirmed by elemental mapping. The prepared samples are studied and compared with their lithium-storage properties using cyclic voltammetry, charge-discharge test and rate performances. The Co-doped V2O5 nanowires have a higher electrochemical lithium-storage capacity than that of the mixed V2O5/Co3O4 nanowires. At a current density of 30 mA g−1, an initial discharge capacity of 624.64 mAh g−1 is achieved in a voltage range of 2–4 V. © 2018 Elsevier B.V.

语种： 英文

作者： Zhou, Zhi;Huang, Shengxiong;Luo, Wei;Wang, Chang;Fan, Xin;...

期刊： Current Nanoscience,2017年13(4):342-348 ISSN：1573-4137

通讯作者： Zhou, Nan;Fang, Dong

作者机构： [Huang, Shengxiong; Luo, Wei; Zhou, Zhi; Zhou, Nan] Hunan Agr Univ, Coll Sci, Changsha, Hunan, Peoples R China.;[Fang, Dong; Wang, Chang; Wan, Renzhuo] Wuhan Text Univ, Coll Mat Sci & Engn, Minist Educ, Key Lab New Text Mat & Applicat Hubei Prov, Wuhan, Peoples R China.;[Fan, Xin] Guilin Univ Technol, Coll Mat Sci & Engn, Guangxi Minist Prov Jointly Constructed Cultivat, Guilin 541004, Peoples R China.;[Fang, Dong] Fac Wuhan Text Univ, Dept Mat Sci & Engn, Wuhan, Peoples R China.

通讯机构： [Zhou, Nan] H;[Fang, Dong] F;Hunan Agr Univ, Coll Sci, Changsha, Hunan, Peoples R China.;Fac Wuhan Text Univ, Dept Mat Sci & Engn, Wuhan, Peoples R China.

关键词： Bismuth nanowires;Bi/C nanocomposites;electrochemistry;Li-ion battery;Na-ion battery

摘要： Background: Bismuth (Bi) has been studied due to its high theoretical gravimetric capacity of 385 mAh g^-1, which is as important as gravimetric capacity for the practical application of battery systems in electronic mobile devices. However, there have been limited fundamental explorations on the electrochemical performances of Bi. Furthermore, the mechanism differences for the Bi anodes in lithium-ion batteries (LIBs) and sodium-ion batteries (NIBs) should be further investigated. Methods: The Bi nanowires were fabricated by vacuum melting and pressure injection method. Briefly, a Bi bulk was placed into an injection apparatus and heated up above 275 &deg;C (melt point of Bi: 271.3 &deg;C), and then the melt was injected into the AAO pores by a hydraulic force. After the injection process, the chamber was kept in vacuum to cool down slowly. Subsequently, the AAO template was dissolved away slowly in the etching solution (0.4 M H<inf>3</inf>PO<inf>4</inf>+ 0.2 M CrO<inf>3</inf>) at 60 &deg;C for 72 h to expose Bi nanowires. Finally, after sonication dispersion, centrifugal sedimentation and rinsing with deionized water several times to remove the excess H<inf>3</inf>PO<inf>4</inf>-CrO<inf>3</inf>mixture, the free-standing Bi nanowires were collected. Results: The morphologies of AAO, AAO/Bi and Bi nanowires were tested and presented in detail. It found that the Bi nanowires can be obtained by pressure injection method followed with dissolve the AAO template. After boll milling with C to form Bi/C nanocomposites, the nanocomposites were assembled as an electrode of LIBs or NIBs. It exhibited high capacities in LIBs, while for NIBs, the capacity retention was relatively low. Conclusion: Bi nanowires have been prepared by mechanical pressure injection method and thoroughly dissolution of the AAO template. After successive milling of Bi nanowires with carbon black, Bi/C nanocomposites are obtained. The Bi/C nanocomposites used as electrode in LIBs exhibit high capacities and the initial discharge/charge capacities of Bi/C anode are around 1223.4/571.9, 905.9/412.3, 829.2/ 362.6 mAh g^-1at current densities of 20, 200 and 500 mA g^-1, respectively. The enhanced electrical performances are attributed to the smaller size of Bi nanowires and the introduced carbon black to buffer the volume changes during discharge/charge process. In NIBs, the capacity retention after 50 cycles reaches 284.7, 196.2 and 168.3 mAh g^-1at current densities of 20, 200 and 500 mA g-1, respectively. Furthermore, in LIBs, Bi and Li⁺ions combine together through an alloying process, while in NIBs, only an intercalation process occurs for Bi and Na+ without indication of alloying.<br/> &copy;2017 Bentham Science Publishers.

语种： 英文

作者： 张亚;王翀;刘双清;廖晓兰;戚培培

期刊： 农业灾害研究,2017年7(6):50-57 ISSN：2095-3305

作者机构： 湖南农业大学植物保护学院, 湖南长沙 410128;植物病虫害生物学与防控湖南省重点实验室, 湖南长沙 410128;湖南农业大学理学院,湖南长沙,410128;湖南农业大学植物保护学院,湖南长沙,410128;湖南省生物农药与农药制剂加工工程技术研究中心, 湖南长沙410128

关键词： 农药学;SCI期刊;收录情况;影响因子

摘要： 为了解农药学期刊被SCI收录的情况和影响因予，以及我国农药学工作者在SCI期刊上发表论文的情况，根据美国科学情报研究所（ISI）2016年在网上发布的农药学领域期刊以及全国40多所农科院校农药学工作者发表论文情况，分别从期刊名称、出版地、出版周期、年论文量、影响因子、期刊类型、分区情况等方面系统整理了适合农药学工作者投稿的期刊，以期为我国农药学及其他相关学科工作者向SCI期刊上投稿提供一定的参考。

语种： 中文

作者： Wei, Qiaoling;Zhang, Feng;Zhao, Xiaofang;Wang, Chao;Xiao, Jianliang;...

期刊： Organic & Biomolecular Chemistry,2017年15(26):5468-5471 ISSN：1477-0520

通讯作者： Tang, Weijun

作者机构： [Wei, Qiaoling; Zhao, Xiaofang; Wang, Chao; Tang, Weijun; Xiao, Jianliang] Shaanxi Normal Univ, Key Lab Appl Surface & Colloid Chem, Minist Educ, Xian 710062, Peoples R China.;[Wei, Qiaoling; Zhao, Xiaofang; Wang, Chao; Tang, Weijun; Xiao, Jianliang] Shaanxi Normal Univ, Sch Chem & Chem Engn, Xian 710062, Peoples R China.;[Xiao, Jianliang] Univ Liverpool, Dept Chem, Liverpool L69 7ZD, Merseyside, England.;[Zhang, Feng] Hunan Agr Univ, Coll Sci, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Tang, Weijun] S;Shaanxi Normal Univ, Key Lab Appl Surface & Colloid Chem, Minist Educ, Xian 710062, Peoples R China.;Shaanxi Normal Univ, Sch Chem & Chem Engn, Xian 710062, Peoples R China.

关键词： Amino acids;Esters;Hydrogenation;Diastereoselective hydrogenation;Glycine derivatives;High yield;Ketimines;Keto ester;Rubidium compounds;glycine;ruthenium;sulfonium derivative;analogs and derivatives;catalysis;chemistry;conformation;hydrogenation;stereoisomerism;synthesis;Catalysis;Glycine;Hydrogenation;Molecular Conformation;Ruthenium;Stereoisomerism;Sulfonium Compounds

摘要： A ruthenium pincer catalyst has been shown to be highly efficient for the hydrogenation of a wide range of alpha-ketimino esters derived from alpha-keto esters and chiral 2-methylpropyl-2-sulfinamide, affording chiral aryl glycine derivatives with high yields and diasteroselectivities (20 examples, dr values up to 99 : 1).

语种： 英文

作者： 王翀;张亚

期刊： 科教导刊,2017年(10):147-148 ISSN：1674-6813

作者机构： 湖南农业大学理学院 湖南·长沙 410128;湖南农业大学植物保护学院 湖南·长沙 410128;植物病虫害生物学与防控湖南省重点实验室 湖南·长沙 410128;湖南省生物农药与农药制剂加工工程技术研究中心 湖南·长沙 410128;[张亚; 王翀] 湖南农业大学

关键词： 低头族;手机;网络;方法

摘要： 随着互联网的发展,智能手机的普及,大学生在课堂上低头玩手机的现象越发严重,成为课堂教学质量下降的重要原因之一。由于时代背景不同,现代大学生获取信息的手段和途径多样,传统的教学方法已不能满足课堂需求,迫切需要改革。本文分析了低头的成因,指出了存在的问题,提出了解决问题的对策,旨在为今后课堂教学改革提供理论依据。

语种： 中文

作者： Liu, S. Q.;Zhang, Y.*;Wang, C.;Su, P.;Liao, X. L.;...

期刊： EGYPTIAN JOURNAL OF BIOLOGICAL PEST CONTROL,2016年26(3):643-650 ISSN：1110-1768

通讯作者： Zhang, Y.

作者机构： [Liao, X. L.; Liu, S. Q.; Zhang, Y.; Bai, L. Y.] Hunan Agr Univ, Coll Plant Protect, Changsha, Hunan, Peoples R China.;[Zhang, Y.] Hunan Prov Key Lab Biol & Cont Plant Dis & Plant, Changsha, Hunan, Peoples R China.;[Wang, C.] Hunan Agr Univ, Coll Sci, Changsha, Hunan, Peoples R China.;[Su, P.] Hunan Acad Agric Sci, Inst Plant Prot, Changsha, Hunan, Peoples R China.

通讯机构： [Zhang, Y.] H;Hunan Agr Univ, Coll Plant Protect, Changsha, Hunan, Peoples R China.;Hunan Prov Key Lab Biol & Cont Plant Dis & Plant, Changsha, Hunan, Peoples R China.

关键词： Amaranthus tricolor;Antibacterial activity;Component isolation;Plant pathogenic bacteria;Structure identification

摘要： The aim of this study was to investigate the antibacterial activity of Amaranthus tricolor leaf extracts and to identify its antibacterial components. The antibacterial activities of A. tricolor leaf extracts against Acidovorax avenae subsp. citrulli, Xanthomonas axonopodis pv. citri, Erwinia carotovora, Pseudomonas solanacearum and Xanthomonas oryzae pv. oryzae, using the Oxford cup method was evaluated. The effect of A. tricolor leaf extracts on the structure of A. avenae subsp. citrulli was determined by scanning electron microscopy. The further purified A. tricolor leaf extract was obtained by using column and thin layer chromatography. The components of purified extract were separated by high-performance liquid chromatography. The active component of purified extract was identified by mass spectrum and nuclear magnetic resonance. The ratio of ethyl acetate and the leaf powder of 10:1 showed the best antibacterial activity. The inhibition zone against X.axonopodis pv. citri of (28.25mm) was the largest at p &lt; 0.05 among the five tested bacteria. A. tricolor leaf extracts destroyed the membranes of A. avenae subsp. citrulli. Purified extract showed good stability against thermo, storage, light and pH. Moreover, the active antibacterial component of A. tricolor leaf extracts was palmitic acid (C16H32O2, 256.42). This study proved that A. tricolor leaf extracts have antibacterial activity against plant pathogenic bacteria. © 2016 Egyptian Society for Biological Control of Pests. All rights reserved.

语种： 英文

作者： Xia, R. Z.;Liu, S. Q.;Xie, Y.;Zhang, Y.*;Wang, C.;...

期刊： EGYPTIAN JOURNAL OF BIOLOGICAL PEST CONTROL,2016年26(1):157-162 ISSN：1110-1768

通讯作者： Zhang, Y.;Liao, X. L.

作者机构： [Yin, W.; Xie, Y.; Liu, S. Q.; Xia, R. Z.] Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.;[Zhang, Y.] Hunan Prov Key Lab Biol & Control Plant Dis & Pla, Changsha 410128, Hunan, Peoples R China.;[Liao, X. L.] Hunan Biol Pesticides & Pesticide Preparat Proc E, Changsha 410128, Hunan, Peoples R China.;[Wang, C.] Hunan Agr Univ, Coll Sci, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Zhang, Y.; Liao, X. L.] H;Hunan Prov Key Lab Biol & Control Plant Dis & Pla, Changsha 410128, Hunan, Peoples R China.;Hunan Biol Pesticides & Pesticide Preparat Proc E, Changsha 410128, Hunan, Peoples R China.

关键词： Amaranthus tricolor;Antibacterial substance;Growth inhibition;Phytopathogenic bacteria

摘要： Efficacy of Amaranthus tricolor extracts were investigated as new bacterial antifungal components. Antibacterial substances were successively extracted from A. tricolor by ethyl acetate. Inhibition zone method was used to study the antibacterial activity of the extract against five phytopathogenic bacteria, i.e., Xanthomonas axonopodis pv. citri, Xanthomonas oryzae pv. oryzicola, Ralstonia solanacearum, Acidovorax avenae subsp. sitrulii, and Erwinia carotovora. At the same time, the factors influencing antibacterial activity such as extraction method and extracting solvent were evaluated. Inhibition rate recorded 24.8–62.1%, with the greatest effects against X. axonopodis pv. citri and X. oryzae pv. oryzicola (62.1 and 58.7%, respectively). The extracts also inhibited X. axonopodis pv. citri in vitro. Generally, the antibacterial substances from A. tricolor were effective against all of the phytopathogens tested. Further study found that different solvents had different influences on extracts. The effective inhibition rate varied by solvent as follows: ethyl acetate > ethanol> acetone = petroleum ether; the ethyl acetate extract significantly inhibited X. axonopodis pv. citri, at a rate almost equal to the control medicament of 4 mg/ml streptomycin. Dissolved the extracts in different solvents showed that distilled water was the most stable, and the effective inhibition rate for X. axonopodis pv. citri varied as follows after 72 h: distilled water (41.2%) > ethanol–water mixture (1:5) (32.4%) > ethanol (27.2%). The results showed that the A. tricolor extracts consisted of a fat-soluble component and a water-soluble substance. Different extraction conditions also had different effects, with oscillating waves had greatest effect and ultrasonication had little impact. Obtained results revealed that extracts from leaves had higher levels of antibacterial substances than from roots and stems (leaf > root > stem). © 2016, Egyptian Society for Biological Control of Pests. All rights reserved.

语种： 英文

作者： 张亚;王翀;孙田;刘双清;廖晓兰

期刊： 化工时刊,2016年30(8):38-40 ISSN：1002-154X

作者机构： 湖南农业大学植物保护学院,湖南长沙410128;植物病虫害生物学与防控湖南省重点实验室,湖南长沙410128;湖南农业大学理学院,湖南长沙,410128;湖南农业大学植物保护学院,湖南长沙,410128;湖南省生物农药与农药制剂加工工程技术研究中心,湖南长沙410128

关键词： 互动式课堂;学时;安排;对策

摘要： 互动式课堂教学模式是一种教师与学生之间交流互动的新型教育模式。该教学模式克服了传统教学模式的弊端,极大地调动了学生上课的积极性,提高了教学质量,越来越受师生的喜爱。然而,该教学模式应用尚不成熟,存在不少问题,本文针对课堂教学过程中,学时不足问题,重点讨论了不足的原因及对策,明确了提高课堂效率的其他措施,并对未来教学改革进行了展望。

语种： 中文

作者： Zhang, Y.*;Wang, C.;Su, P.;Liao, X. L.;Liu, S. Q.

期刊： EGYPTIAN JOURNAL OF BIOLOGICAL PEST CONTROL,2016年26(2):387-393 ISSN：1110-1768

通讯作者： Zhang, Y.

作者机构： [Liu, S. Q.; Zhang, Y.] Hunan Agr Univ, Coll Plant Protect, Dept Plant Protect, Changsha, Hunan, Peoples R China.;[Wang, C.] Hunan Agr Univ, Coll Sci, Dept Chem, Changsha, Hunan, Peoples R China.;[Su, P.] Hunan Acad Agr Sci, Inst Plant Protect, Lab Biocontrol, Changsha, Hunan, Peoples R China.;[Liao, X. L.] Hunan Prov Key Lab Biol & Control Plant Dis & Pla, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Zhang, Y.] H;Hunan Agr Univ, Coll Plant Protect, Dept Plant Protect, Changsha, Hunan, Peoples R China.

关键词： Antibiotic compound;Phenazine-1-carboxamide;Pseudomonas aeruginosa;Rhizoctoniasolani;Xanthomonas

摘要： Plant pathogenic fungi, Rhizoctonia solani and bacteria, such as Xanthomonas oryzae pv. oryzicola and X. campestris pv. citri, caused critical loss in agriculture. Developing new alternative biological agents with high efficiency and low toxicity is needed. In this work, An antagonistic Pseudomonas aeruginosa strain SU8 from a farm breeding ducks and growing rice simultaneously was isolated. It was identified according to its 16S rRNA gene and morphological, physiological and biochemical characteristics. Also, an antibiotic compound F125 (phenazine-1-carboxamide) was separated from its ethyl acetate extract. F125 showed 83.63% grow inhibition rate against R. solani at 0.01 mg/ml concentration, meanwhile, EC50 of it was 0.7863 mg/ml and 1.5661 mg/ml against X. oryzae pv. oryzicola and X. campestris pv. citri, respectively. Furthermore, F125 showed high safety for rice and mice. Rice could sprout and grow normally when F125 concentration was below 0.06 mg/ml and 0.1 mg/ml, respectively. Also, acute toxicity of F125 against mice resulted in LD50&gt; 5 mg/g BW (body weight). In a whole, F125 displayed a promising prospect in the crop disease prevention. © 2016 Egyptian Society for Biological Control of Pests. All rights reserved.

语种： 英文

作者： 蒋忠洪;王翀;张亚;廖晓兰;邵占松

期刊： 农业灾害研究,2016年6(8):23-25 ISSN：2095-3305

作者机构： 湖南农业大学植物保护学院,湖南长沙,410128;湖南农业大学理学院,湖南长沙,410128;湖南农业大学植物保护学院,湖南长沙 410128;湖南农业大学理学院,湖南长沙 410128;植物病虫害生物学与防控湖南省重点实验室,湖南长沙 410128

关键词： 啶酰菌胺;氟啶胺;复配物;草莓灰霉病菌;抑制作用

摘要： 为评价啶酰菌胺和氟啶胺复配物对草莓灰霉病菌的增效作用,开发防治草莓灰霉病的新药剂,采用生长速率法测定了啶酰菌胺和氟啶胺复配物对草莓灰霉病菌的室内毒力,并采用Wadley法评价了复配物的增效潜力。结果表明,啶酰菌胺和氟啶胺质量比为1∶1、1∶5、5∶1、3∶1、2∶3时表现为相加作用,其增效系数分别为1.09、0.67、0.73、0.56、0.56;啶酰菌胺和氟啶胺质量比为1∶2、1∶3、2∶1、3∶2时表现为拮抗作用,其增效系数分别为0.44、0.42、0.41、0.48。说明啶酰菌胺和氟啶胺在一定条件下复配能提高对草莓灰霉病菌的控制效果,但复配比例不当则会降低对该病菌的控制效果。

语种： 中文

作者： Zhang, Y. J.*;Wang, C.;Zhang, Y.;Liu, S. Q.;Su, P.;...

期刊： EGYPTIAN JOURNAL OF BIOLOGICAL PEST CONTROL,2016年26(4):727-732 ISSN：1110-1768

通讯作者： Zhang, Y. J.

作者机构： [Liao, X. L.; Zhang, Y. J.; Liu, S. Q.; Zhang, Y.] Hunan Agr Univ, Coll Plant Protect, Changsha, Hunan, Peoples R China.;[Wang, C.] Hunan Agr Univ, Coll Sci, Changsha, Hunan, Peoples R China.;[Zhang, Y.] Hunan Prov Key Lab Biol & Cont Plant Dis & Plant, Changsha, Hunan, Peoples R China.;[Su, P.] Hunan Acad Agr Sci, Inst Plant Prot, Changsha, Hunan, Peoples R China.

通讯机构： [Zhang, Y. J.] H;Hunan Agr Univ, Coll Plant Protect, Changsha, Hunan, Peoples R China.

关键词： Antagonistic bacteria;Identification;Inhibitory effects;Rhizoctonia solani;Screening

摘要： The bacterial strain Alcaligenes faecalis B137W, which has antagonism against Rhizoctonia solani, was firstly screened out from an integrated rice-duck ecosystem field by the dual culture method. Morphological, physiological and biochemical characteristics as well as the sequence of the 16S rDNA gene and phylogenetic analysis showed that the strain B137W, whose 16S rDNA sequence has been registered in Gene Bank (accession number KC199967). It belongs to Alcaligenes faecalis, and has high homology with the model strain Alcaligenes faecalis ATCC8750. This strain obtained one national patent from China: ZL201310305517.7. The fermented filtrate of B137W was used to test its inhibitory effect on fungal plant pathogens. The results showed that it has an inhibitory rate of 60.26% against Rhizoctonia solani within 48 h, without significant differences in the rate of validamycin. It also showed a broad range of inhibitory effects on Pyricularia grisea, Fusarium graminearum, Ustilaginoidea virens and Sclerotinia sclerotiorum. © 2016, Egyptian Society for Biological Control of Pests. All rights reserved.

语种： 英文

作者： 刘双清;王翀;张亚;廖晓兰;马文月;...

期刊： 中国蔬菜,2016年(7):37-43 ISSN：1000-6346

作者机构： 湖南农业大学植物保护学院，湖南长沙 410128;植物病虫害生物学与防控湖南省重点实验室，湖南长沙 410128;湖南农业大学理学院,湖南长沙,410128;湖南农业大学植物保护学院,湖南长沙,410128;湖南省生物农药与农药制剂加工工程技术研究中 心，湖南长沙 410128

关键词： 湖南省;草莓灰霉病菌;遗传多样性;SSR标记

摘要： 为探明湖南省草莓灰霉病菌群体遗传结构、分化及变异规律，采用荧光标记技术对湖南省9个地理菌群的180株草莓灰霉病菌基因组DNA进行SSR分析。结果表明：9对SSR引物共检测到56个观察等位基因（Na），平均值为6.22；有效等位基因（Ne）为1.27～4.89，平均值为3.44；不同位点的基因多样性指数（H）为0.21～0.80，平均值为0.65；不同位点Shannon’s信息指数（I）为0.37～1.83，平均值为1.34。供试180株样品9个SSR多态性位点上多态性信息量PIC变化范围为0.13～0.91，不同位点PIC差异大，这一规律与I和H基本一致。不同SSR位点总的遗传多样性（Ht）为0.21～0.77，平均值为0.65；群内遗传多样性（Hs）为0.19～0.50，平均值为0.40；遗传分化系数（Gst）为0.10～0.46，平均值为0.37；基因流（Nm）为0.58～4.47，平均值为1.16。不同地理菌群平均观察等位基因（Na）、有效等位基因数目（Ne）分别为2.93和2.07，Shannon’s信息指数（I）平均为0.67，基因多样性指数（H）平均为0.40，平均多态性位点数（NP）、多态位点百分率（P）分别为6.78和75%。依据不同地理菌群之间的遗传距离（0.2797～1.9225），将供试湖南省9个地理菌群分为4大类：第Ⅰ大类主要由长沙、邵阳、岳阳、衡阳、张家界、湘潭种群组成；第Ⅱ大类主要由郴州种群组成；第Ⅲ大类主要由株洲种群组成；第Ⅳ大类由常德种群组成。总之，湖南省各地草莓灰霉病菌群体遗传多样性丰富，但地理群体间差异小，病菌遗传变异主要来自群体内部，不同地区间存在病菌的移动。

语种： 中文

作者： 张亚;王翀;杨茜;刘双清;廖晓兰

期刊： 化工时刊,2016年30(5):43-46 ISSN：1002-154X

作者机构： 湖南农业大学植物保护学院,湖南 长沙 410128;植物病虫害生物学与防控湖南省重点实验室,湖南 长沙 410128;湖南农业大学理学院,湖南 长沙,410128;湖南农业大学植物保护学院,湖南 长沙,410128;湖南省生物农药与农药制剂加工工程技术研究中心,湖南 长沙 410128

关键词： 大学生;低头族;危害;成因;对策

摘要： 随着智能手机和网络的普及,越来越多的人喜欢从手机探寻信息,于是"低头族"便形成了,尤其在高校,"低头族"现象十分常见,其危害也很严重。如何能把大学生从低头族变为"抬头族",提高大学生的学习效果和知识素质成为全体高校教师和教育部门关注的重要内容之一。本文介绍了"低头族"的定义,分析了其危害,重点讨论了成因,提出了解决问题的对策,旨在为高校课堂教学改革提供理论依据。

语种： 中文