作者： Jin, Jisu;Li, Youzhi;Zhou, Zhongshi;Zhang, Hong;Guo, Jianying*;...

期刊： Frontiers in Physiology,2020年11:562204 ISSN：1664-042X

通讯作者： Wan, Fanghao;Guo, Jianying

作者机构： [Li, Youzhi; Jin, Jisu; Wan, Fanghao] Hunan Agr Univ, Coll Plant Protect, Changsha, Peoples R China.;[Guo, Jianying; Wan, Fanghao; Zhou, Zhongshi; Jin, Jisu; Zhang, Hong] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing, Peoples R China.

通讯机构： [Wan, Fanghao] H;[Guo, JY; Wan, FH] C;Hunan Agr Univ, Coll Plant Protect, Changsha, Peoples R China.;Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing, Peoples R China.

关键词： Agasicles hygrophila;Heat shock protein (HSP);Heat shock factor (HSF);RNAi;thermotolerance

摘要： Heat shock proteins are molecular chaperones that are involved in numerous normal cellular processes and stress responses, and heat shock factors are transcriptional activators of heat shock proteins. Heat shock factors and heat shock proteins are coordinated in various biological processes. The regulatory function of heat shock factors in the expression of genes encoding heat shock proteins (Hsps) has been documented in some model insects, however, the role of transcription factors in modulating Hsps in other insects is still limited. In this study, one heat shock factor gene (AhHsf) was isolated and its two potential target genes (AhHsp70 and AhsHsp21) were confirmed from Agasicles hygrophila. AhHsf sequence analysis indicated that it belongs to the Hsfs gene family. RT-qPCR showed that expression levels of heat shock factors and of two heat shock proteins significantly increased under heat stress. Injection with double-stranded Hsf RNA in freshly emerged adult beetles significantly inhibited expression of AhHsp70 and AhsHsp21, shortened the adult survival, drastically reduced egg production, and ultimately led to a decrease in fecundity. RNA interference (RNAi)-mediated suppression of AhHsp70 or AhsHsp21 expression also significantly affected expression of AhHsf. Our findings revealed a potential transcriptional function of AhHsf to regulate expression of AhHsp70 and AhsHsp21, which may play a key role in A. hygrophila thermotolerance. Our results improve our understanding of the molecular mechanisms of the AhHsf - AhHsps signaling pathway in A. hygrophila. © Copyright © 2020 Jin, Li, Zhou, Zhang, Guo and Wan.

语种： 英文

作者： Wu, Shaolong;Deng, Wan;Li, Mi;Xiao, Yansong;Li, Jiaying;...

期刊： Frontiers in Physiology,2020年11:588291 ISSN：1664-042X

通讯作者： Li, Youzhi;Zhou, Zhicheng

作者机构： [Wu, Shaolong; Li, Youzhi] Hunan Agr Univ, Coll Plant Protect, Changsha, Peoples R China.;[Wu, Shaolong; Teng, Kai; Xiao, Zhipeng; Zhou, Zhicheng; Xiao, Yansong; Li, Jiaying] Hunan Prov Tobacco Co, Changsha, Peoples R China.;[Li, Mi; Deng, Wan] Hunan Acad Forestry, Changsha, Peoples R China.;[Li, Xiaohong] Shaoyang Univ, Coll Urban & Rural Construct, Shaoyang, Peoples R China.

通讯机构： [Li, Youzhi; Zhou, Zhicheng] H;Hunan Agr Univ, Coll Plant Protect, Changsha, Peoples R China.;Hunan Prov Tobacco Co, Changsha, Peoples R China.

关键词： ：Arma chinensis;Chemoreception genes;Transcriptome;gender;olfactory system

摘要： The predatory insect Arma chinensis (Hemiptera: Pentatomidae) is widely distributed in China, where it is also used to control many agricultural and forest pests. The chemosensory genes expressed in its antennae play crucial roles in food-seeking and mating behaviors. To better understand the olfaction of A. chinensis antennae, we identified the genes related to food-seeking and mating. Sequencing of the antennal transcriptomes of full and hungry male and female A. chinensis revealed 38 odorant-binding proteins (OBPs), 1 chemosensory protein (CSP), 1 Niemann–Pick C2 protein (NPC2), 3 odorant receptors (ORs), 12 ionotropic receptors (IRs), 2 gustatory receptors (GRs), and 3 sensory neuron membrane proteins (SNMPs). These results were used to construct phylogenetic trees. A quantitative real-time PCR (qRT-PCR) analysis showed that the relative transcript levels of AchiGR1, AchiGR2, and AchiOBP28 were higher in female than in male antennae in both full and hungry insects, but that the expression of AchiOBP13 and AchiOBP16 was higher only in full A. chinensis females. Thus, the latter genes may encode proteins involved in oviposition selection behavior. AchiGRs (1 and 2), AchiIR6, and AchiOBPs (6–8, 12, 20–22, 28, and 34) were highly expressed only in the antennae of full males, indicating the participation of these genes in mate-searching or male pheromone recognition. The expression of AchiOBP31 in the antennae of starved males, AchiOBPs (15, 18, and 29) in the antennae of starved females, and AchiOBPs (3, 4, and 24) in the antennae of starved males and females suggested that these genes encode food-seeking functions. Our identification of chemosensory genes in A. chinensis antennae and their differential expression in full and hungry insects provides the basis for further functional studies on the chemoreception system of A. chinensis and the sex hormones of predatory insects. © Copyright © 2020 Wu, Deng, Li, Xiao, Li, Teng, Xiao, Li, Zhou and Li.

语种： 英文

作者： 伍绍龙;邓婉;蔡海林;杨佳蒴;曾维爱;...

期刊： 中国生物防治学报,2020年36(02):169-174 ISSN：2095-039X

作者机构： 湖南农业大学植物保护学院,长沙410128;湖南省烟草公司,长沙410004;湖南省林业科学院,长沙410004;湖南省烟草公司长沙市公司,长沙410011;[周志成; 杨佳蒴] 中国烟草总公司湖南省公司

关键词： 蠋蝽;饥饿;饲养密度;种内互残;发生时期

摘要： 为明确饥饿情况下蠋蝽种内残杀行为的发生时期和影响因素,指导蠋蝽规模化繁育和释放,本文研究了不同发育时期和密度下的蠋蝽种内残杀行为。研究发现:蠋蝽对卵粒的种内互残行为主要发生在饥饿24 h以内,雌性蠋蝽对卵粒的取食行为显著强于雄性蠋蝽,取食比例是雄性的2.80~3.25倍、取食量是雄性的18.75~21.20倍,雌性取食下的卵粒羽化率为70.66%,显著低于雄性和对照的86.59%和87.88%。发育时期和饲养密度均显著影响蠋蝽的种内残杀行为,主要发生在饥饿后的19.63~36.00 h,并且1、2、3龄若虫没有表现出种内残杀行为,4龄以后种内残杀行为显著;低密度下蠋蝽种内互残行为不显著,但高密度会增强种内互残行为,且随密度的增加,种内互残行为显著增强。

语种： 中文

作者： Hu, Kui;Tian, Ping;Yang, Lu;Tang, Yan;Qiu, Lin;...

期刊： Molecular Biology Reports,2020年47(2):1099-1106 ISSN：0301-4851

通讯作者： Li, Youzhi

作者机构： [Tian, Ping; Qiu, Lin; Li, Youzhi; Yang, Lu; Hu, Kui; Tang, Yan; He, Hualiang] Hunan Agr Univ, Coll Plant Protect, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha 410128, Hunan, Peoples R China.;[Ding, Wenbing] Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Hunan, Peoples R China.;[Li, Youzhi; Ding, Wenbing] Hunan Agr Univ, Hunan Prov Engn & Technol Res Ctr Biopesticide &, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Li, Youzhi] H;Hunan Agr Univ, Coll Plant Protect, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Hunan Prov Engn & Technol Res Ctr Biopesticide &, Changsha 410128, Hunan, Peoples R China.

关键词： Kruppel homolog 1 protein;transcription factor;unclassified drug;kruppel like factor;adult;amino acid sequence;animal tissue;Article;controlled study;Delphacidae;DNA synthesis;female;imago;male;molecular phylogeny;nonhuman;open reading frame;ovary development;phylogenetic tree;protein domain;protein expression;RNA interference;RNA isolation;sequence analysis;Sogatella furcifera;animal;classification;DNA sequence;embryology;gene expression regulation;gene knockdown;genetics;Hemiptera;metabolism;molecular cloning;organogenesis;ovary;phylogeny;Amino Acid Sequence;Animals;Cloning, Molecular;Female;Gene Expression Regulation, Developmental;Gene Knockdown Techniques;Hemiptera;Kruppel-Like Transcription Factors;Organogenesis;Ovary;Phylogeny;RNA Interference;Sequence Analysis, DNA

摘要： Juvenile hormone (JH) plays a pivotal role in insect reproduction. The Krüppel-homolog 1 (Kr-h1) is a JH-inducible zinc finger transcription factor that has also been found to play a role in insect reproduction, however, its function varies across species. In this study, we cloned SfKr-h1 from Sogatella furcifera and investigated its role in ovarian development. The open reading frame of SfKr-h1 is 1 800bp encoding 599 amino acids. The putative amino acid sequence of SfKr-h1 contains eight putative C2H2-type zinc finger domains and is highly homologous with the Kr-h1s of other hemipteran species. Expression of SfKr-h1 peaked 96h after adult emergence and was highest in the ovary. RNA interference (RNAi) knockdown of SfKr-h1 substantially reduced the transcription of SfVg, and arrested ovarian development. These results suggest that SfKr-h1 is critical for normal ovarian development in S. furcifera. © 2019, Springer Nature B.V.

语种： 英文

作者： 李叶;刘胜;张凯;张瑶;李有志;...

期刊： 天然产物研究与开发,2019年31(4):633-637 ISSN：1001-6880

作者机构： 湖南农业大学植物保护学院;国家植物功能成分利用工程技术研究中心, 国家植物功能成分利用工程技术研究中心, 长沙, 410128;[张凯; 刘胜; 张瑶; 李叶; 丁文兵; 李有志] 湖南农业大学

关键词： 淡紫拟青霉菌;灰毛豆;内生真菌;抑菌活性

摘要： 从灰毛豆叶片中分离出内生真菌-淡紫拟青霉菌Purureocillium lilacinum (TPL04),对其发酵产物采用硅胶柱色谱、SephadexLH-20凝胶色谱、制备薄层色谱、中压柱色谱及制备液相色谱等方法进行分离纯化,共获得8个单体化合物.通过波谱学方法并结合相关文献数据比对,化合物结构鉴定为5-羟基己酸-4-内酯(1)、(R)-4-苄基-2-噁唑烷酮(2)、2'-脱氧胸苷(3)、对羟基苯甲酸甲酯(4)、光色素(5)、β-胡萝卜苷(6)、尿嘧啶核苷(7)、己六醇(8).其中化合物1、2、4是首次从淡紫拟青霉菌中分离得到,抑菌实验表明化合物4对水稻纹枯菌(Pellicularia sasakii)、辣椒疫霉菌(Phytophthora capsici)、草莓灰霉菌(Botrytis cinerea)、油菜菌核菌(Sclerotinia sclerotiorum)四种植物病原菌生长有抑制作用.

语种： 中文

作者： Tian, Ping;Qiu, Lin;Zhou, Ailin;Chen, Guo;He, Hualiang;...

期刊： JOURNAL OF ECONOMIC ENTOMOLOGY,2019年112(5):2207-2214 ISSN：0022-0493

通讯作者： Li, Youzhi

作者机构： [Tian, Ping; Qiu, Lin; Li, Youzhi; Zhou, Ailin; Chen, Guo; He, Hualiang] Hunan Agr Univ, Coll Plant Protect, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha 410128, Hunan, Peoples R China.;[Li, Youzhi; Ding, Wenbing] Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Hunan, Peoples R China.;[Ding, Wenbing] Hunan Prov Engn & Technol Res Ctr Biopesticide &, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Li, Youzhi] H;Hunan Agr Univ, Coll Plant Protect, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Hunan, Peoples R China.

关键词： Chlorops oryzae;Cadherin;normalization;qRT-PCR;reference genes

摘要： Reverse transcription quantitative polymerase chain reaction (PCR) has become an invaluable technique for analyzing gene expression in many insects. However, this approach requires the use of stable reference genes to normalize the data. Chlorops oryzae causes significant economic damage to rice crops throughout Asia. The lack of suitable reference genes has hindered research on the molecular mechanisms underlying many physiological processes of this species. In this study, we used quantitative real-time PCR to evaluate the expression of eight C. oryzae housekeeping genes glyceraldehyde-3-phosphate dehydrogenase (GAPDH), beta-actin (betaACT), beta-tubulin (betaTUB), Delta Elongation factor-1 (EF1delta), ribosomal protein S11 (RPS11), RPS15, C-terminal-Binding Protein (CtBP), and ribosomal protein 49 (RP49) in different developmental stages and tissues in both larvae and adults. We analyzed the data with four different software packages: geNorm, NormFinder, BestKeeper, and RefFinder and compared the results obtained with each method. The results indicate that PRS15 and RP49 can be used as stable reference genes for quantifying gene expression in different developmental stages and larval tissues. GAPDH and betaACT, which have been considered stable reference genes by previous studies, were the least stable of the candidate genes with respect to larval tissues. GAPDH was, however, the most stable reference gene for adult tissues. We verified the candidate reference genes identified and found that the expression levels of Cadherins (Cads) changed when different reference genes were used to normalize gene expression. This study provides a valuable foundation for future research on gene function, and investigating the molecular basis of physiological processes, in C. oryzae.

语种： 英文

作者： Hu, Kui;Qiu, Lin;Ding, Wenbing;He, Hualiang;Li, Youzhi*

期刊： Molecular Biology Reports,2019年46(4):3945-3953 ISSN：0301-4851

通讯作者： Li, Youzhi

作者机构： [Hu, Kui; Qiu, Lin; He, Hualiang; Li, Youzhi] Hunan Agr Univ, Coll Plant Protect, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha 410128, Hunan, Peoples R China;[Ding, Wenbing; Li, Youzhi] Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Hunan, Peoples R China;[Ding, Wenbing] Hunan Agr Univ, Hunan Prov Engn & Technol Res Ctr Biopesticide &, Changsha 410128, Hunan, Peoples R China

通讯机构： [Li, Youzhi] H;Hunan Agr Univ, Coll Plant Protect, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha 410128, Hunan, Peoples R China. Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Hunan, Peoples R China.

关键词： Southern rice black-streaked dwarf virus;Reference genes;qRT-PCR;Normalization;Isoprenoid metabolic pathway

摘要： The Southern rice black-streaked dwarf virus (SRBSDV), a novel Fijivirus, poses a major threat to rice production in East Asia. Analysis of the gene expression of SRBSDV-infected rice plants may reveal the molecular basis of interactions between the virus, its vector and rice plants. Reliable reference genes are required for accurate qRT-PCR analysis. However, no reliable, valid reference genes for examining gene expression in SRBSDV-infected rice plants have so far been identified. We examined the expression of eight candidate reference genes in the leaves of SRBSDV-infected, and healthy, rice plants at different points in time after virus inoculation. We used four dedicated algorithms, geNorm, BestKeeper, NormFinder and RefFinder, to evaluate the performance of these candidate genes. The RefFinder results indicate that 18S, EF1 alpha and UBQ10 are suitable reference genes. In addition, we used these three reference genes to analyze the expression of key genes involved in the isoprenoid metabolic pathway in rice leaves after infection by SRBSDV. The results of this analysis reveal that SRBSDV may suppress the production of the rice plant volatiles that attract natural enemies of its vector Sogatella furcifera, thereby increasing the likelihood of pathogen transmission.

语种： 英文

作者： Hu, Kui;Yang, Houhong;Liu, Sheng;He, Hualiang;Ding, Wenbing;...

期刊： FLORIDA ENTOMOLOGIST,2019年102(2):353-358 ISSN：0015-4040

通讯作者： Li, Youzhi

作者机构： [Qiu, Lin; Li, Youzhi; Liu, Sheng; Ding, Wenbing; Yang, Houhong; Hu, Kui; He, Hualiang] Hunan Agr Univ, Coll Plant Protect, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha 410128, Hunan, Peoples R China.;[Li, Youzhi; Ding, Wenbing] Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Li, Youzhi] H;Hunan Agr Univ, Coll Plant Protect, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Hunan, Peoples R China.

关键词： gene silencing;host plant choice;olfactory protein;white-backed planthopper

摘要： Southern rice black-streaked dwarf virus, transmitted by the white-backed planthopper (Sogatella furcifera [Horváth]) (Hemiptera: Delphacidae) was first found in Guangdong Province, China. A previous study has demonstrated that the host plant preferences of S. furcifera are altered by infection with the virus, with virus-free S. furcifera preferring virus-infected rice plants over healthy rice plants, whereas viruliferous S. furcifera prefer uninfected plants. To test the hypothesis that odorant-binding proteins (OBPs) are involved in the preference of S. furcifera for virus-infected rice plants, we first compared the expression levels of SfurOBP2 and SfurOBP11 in virus-free and viruliferous S. furcifera. The results show that mRNA transcript of these 2 genes were significantly reduced in viruliferous S. furcifera. We then used RNAi-mediated gene silencing to confirm the function of these 2 odorant-binding proteins in host selection of S. furcifera. The results showed that silencing of the SfurOBP2 gene caused virus-free S. furcifera to no longer prefer virus-infected rice plants over uninfected rice plants, but the ability to locate host plants was maintained. These results indicate that SfurOBP2 appears to play a crucial role in the preference of S. furcifera for virus-infected rice plants. © 2019 Florida Entomological Society. All rights reserved.

语种： 英文

作者： 黄聪;李有志;杨念婉;武强;邢龙生;...

期刊： 植物保护,2019年45(5):112-120,134 ISSN：0529-1542

作者机构： 湖南农业大学植物保护学院;[杨念婉; 武强] 中国农业科学院植物保护研究所;[钱万强; 邢龙生; 席羽] 中国农业科学院深圳农业基因组研究所;浙江大学昆虫科学研究所;[李飞] 浙江大学

关键词： 入侵昆虫;基因组;入侵性

摘要： 随着全球贸易的加速发展,入侵物种对农林业、生态环境及人类健康的威胁日益严重。基因组学研究为阐明外来有害生物入侵的分子机制与生态适应性过程以及研发新型防控技术提供了新手段、新平台与大数据。本文综述了入侵昆虫基因组学研究的发展现状,系统总结了基因/基因家族、转座子/重复序列等基因组信息在决定昆虫入侵性中的重要作用,着力探讨了基因组学研究在助力害虫RNAi、昆虫不育技术(SIT)、化学生态防治和物理防治等防控新技术/新产品开发方面的潜力,并展望了基因组学研究应用于入侵昆虫综合防控的前景。

语种： 中文

作者： 李志文;赵瑞;李有志

期刊： 植物保护,2019年45(5):163-170,201 ISSN：0529-1542

作者机构： 湖南农业大学昆虫研究所,植物病虫害生物学与防控湖南省重点实验室,长沙410128;[李志文; 李有志; 赵瑞] 湖南农业大学

关键词： 油茶象;普通油茶;小果油茶;种群密度;空间格局;最适抽样数

摘要： 油茶象Curculio chinensis Chevrolat是我国特有木本油料树种--油茶Camellia spp.的专性蛀果害虫,常导致其大量落果。本文旨在明确油茶象幼体(卵+幼虫)种群动态、空间格局及其关键影响因子,以便为调查取样、长期监测和科学防控等提供理论依据。2017年在湖南常宁普通油茶林,2016年-2017年在湖南益阳小果油茶林于雌虫产卵季节进行了调查,解剖收集的果实并记录其所含幼体数。用6种聚集度指标(m*/m、I_δ、C、I、C_a、k)和Taylor幂法则、Iwao回归模型分析油茶象幼体的空间格局,并探讨其关键影响因子。调查和分析表明,雌虫于6月上、中旬开始产卵,幼体种群密度呈单峰变化,2016年和2017年高峰期分别在7月25日和7月18日。小果油茶林2016年、2017年高峰期种群密度分别为17.95±1.53和16.27±1.06幼体/样株,高于普通油茶林,分别是普通油茶林种群密度(8.16±1.23幼体/样株)的2.20倍和1.99倍,差异极显著(P<0.001)。聚集度指标分析显示,油茶象在普通油茶林均呈聚集分布,在小果油茶林呈聚集分布或随机分布。回归模型表明,该虫在两种油茶林均呈聚集分布。Blackith种群聚集均数λ>2,表明油茶象幼体聚集是由亲代雌虫本身的产卵行为与寄主果实特征综合作用的结果。给出了最适理论抽样数公式并绘制了3种抽样精度下最适抽样数随种群密度变化的曲线图。综上,初步明确了油茶象幼体在普通油茶林和小果油茶林的空间分布格局和种群动态,并探讨了其关键影响因子。试验结果可为该虫调查取样、长期监测和科学防控等提供理论依据。

语种： 中文

作者： Huang, Shuying;Li, Shanshan;Li, Guanhua;He, Hualiang;Qiu, Lin;...

期刊： Biochemical Systematics and Ecology,2019年83:114-116 ISSN：0305-1978

通讯作者： Li, Youzhi

作者机构： [Qiu, Lin; Li, Guanhua; Li, Youzhi; Ding, Wenbing; Huang, Shuying; Li, Shanshan; He, Hualiang] Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.;[Li, Youzhi; Ding, Wenbing] Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Li, Youzhi] H;Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.

关键词： Berchemiella Nakai;Berchemiella wilsonii;Chemotaxonomy;Megastigmane

摘要： Phytochemical investigation of the aerial parts of Berchemiella wilsonii (Schneid.) Nakai (Rhamnaceae) led to the isolation of four flavonoids (1–4), three phenolic acids (5–7), two megastigmane derivatives (8–9) and one triterpene (10). The structures of these compounds were elucidated as taxifolin (1), (−)-epicatechin (2), quercetin 3-O-a-L-arabinopyranoside (3), vitexin (4), methyl p-hydroxycinnamat (5), 3,4-dihydroxybenzoic acid (6), 2-hydroxy-4-methoxy-3,6-dimethyl benzoic acid (7), (3S,5R,6R,7E,9S)-3,5,6,9-tetrahydroxy-7-en-megastigmane (8), (6S,9R)-roseoside (9) and lupeol (10) on the basis of NMR spectral data and comparison with literature values. These results are the first chemical constituent data of the genus Berchemiella, and the chemotaxonomic significance of these compounds is discussed. © 2019

语种： 英文

作者： Hu, Kui;Liu, Sheng;Qiu, Lin;Li, Youzhi*

期刊： PEERJ,2019年7(3):e6576 ISSN：2167-8359

通讯作者： Li, Youzhi

作者机构： [Qiu, Lin; Li, Youzhi; Liu, Sheng; Hu, Kui] Hunan Agr Univ, Coll Plant Protect, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha, Hunan, Peoples R China.;[Li, Youzhi] Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha, Hunan, Peoples R China.

通讯机构： [Li, Youzhi] H;Hunan Agr Univ, Coll Plant Protect, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha, Hunan, Peoples R China.;Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha, Hunan, Peoples R China.

关键词： acetophenone;beta farnesene;beta ionone;caryophyllene;complementary DNA;limonene;Odorant binding protein;peptides and proteins;unclassified drug;volatile agent;Article;behavior;binding affinity;competitive binding assay;controlled study;Delphacidae;dissociation constant;gene;gene expression;gene knockdown;nonhuman;olfactory system;pest control;protein purification;real time polymerase chain reaction;rice;RNA isolation;sequence alignment;sequence analysis;SfurOBP1 gene;SfurOBP11 gene;SfurOBP2 gene;SfurOBP3 gene;signal transduction;sogatella furcifera

摘要： Plant volatiles play an important role in regulating insect behavior. Odorant binding proteins (OBPs) are involved in the first step of the olfactory signal transduction pathway and plant volatiles recognition. Sogatella furcifera is one of the most destructive pests of rice crops. Understanding the functions of S. furcifera OBPs (SfurOBPs) in the host plant location and the behavioral responses of S. furcifera to rice plant volatiles could lead to improved, more environmentally-friendly, methods for controlling this pest. We found that SfurOBP1 displayed only weak binding with all the tested volatiles. SfurOBP2, SfurOBP3 and SfurOBP11 had different binding affinities to beta-ionone. SfurOBP2 and SfurOBP11 had strong binding affinities to beta-caryophyllene (Ki = 2.23 microM) and plant alcohol (Ki = 2.98 microM), respectively. The results of Y-olfactometer experiments indicate that S. furcifera was significantly repelled by octanal and n-octane but strongly attracted by (+)-limonene, acetophenone, 2-heptanone, n-hendecane, alpha-farnesene and beta-ionone. Furthermore, the dsRNA-mediated gene silencing of SfurOBP2, SfurOBP3 and SfurOBP11 shifted the olfactory behavior of S. furcifera for beta-ionone, alpha-farnesene and plant alcohol, respectively. These results suggest that the SfurOBPs are involved in the recognition of rice plant volatiles, and several potential repellants and lures for controlling this pest.

语种： 英文

作者： Huang Cong;Wu Qiang;Jiang Chun-yan;Xing Long-sheng;Shi Guo-liang;...

期刊： 农业科学学报(英文),2019年18(7):1624-1633 ISSN：2095-3119

通讯作者： Wan Fang-hao

作者机构： [Wan Fang-hao; Li You-zhi; Huang Cong] Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.;[Wan Fang-hao; Wu Qiang; Shi Guo-liang; Jiang Chun-yan; Yang Nian-wan] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China.;[Wan Fang-hao; Xing Long-sheng; Xi Yu; Zhang Bin; Huang Cong; Qian Wan-qiang] Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518120, Peoples R China.;[Shi Guo-liang; Zhang Bin; Jiang Chun-yan] Qingdao Agr Univ, Coll Plant Hlth & Med, Qingdao 266109, Shandong, Peoples R China.

通讯机构： [Wan Fang-hao] H;[Wan Fang-hao] C;Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.;Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China.;Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518120, Peoples R China.

关键词： juvenile hormone esterase;CpJHE-like;codling moth;Cydia pomonella

摘要： Juvenile hormone esterase (JHE) is a key enzyme for insects, playing an important role in the regulation of insect growth, development, diapause and reproduction. We identified a complete putative JHE of Cydia pomonella (CpJHE-like) which is comprised of a 1 761 bp coding sequence (CDS) encoding 587 amino acid residues from the transcriptome data. The deduced protein sequence of CpJHE-like showed the highest identity of 60.44% with the Adoxophyes honmai JHE (AhJHE) and the minimal identity of 25.81% with Aedes aegypti JHE (AaJHE). CpJHE-like exhibited all the seven typical motifs of the functional JHEs and had the highly consistent tertiary structure with Manduca sexta JHE (MsJHE). Phylogenetic analysis showed that the CpJHE-like was close to two JHEs from the family Tortricidae. The CpJHE-like transcript level take a leap in the 3-day-old fifth instar larva, increased about 300-fold compared to the basal level. Tissue-specific expression profile showed that the CpJHE-like transcript was expressed mainly in the fat body. This study indicates that the CpJHE-like is the functional JHE, which may play vital roles in the development and reproduction of C. pomonella. © 2019 CAAS. Publishing services by Elsevier B.V

语种： 英文

作者： Hu, Kui;Tian, Ping;Yang, Lu;Qiu, Lin;He, Hualiang;...

期刊： Journal of Insect Science,2019年19(6) ISSN：0970-3837

通讯作者： Li, Youzhi

作者机构： [Tian, Ping; Qiu, Lin; Li, Youzhi; Yang, Lu; Hu, Kui; He, Hualiang] Hunan Agr Univ, Coll Plant Protect, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha 410128, Peoples R China.;[Ding, Wenbing] Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Peoples R China.;[Li, Youzhi; Ding, Wenbing] Hunan Agr Univ, Hunan Prov Engn & Technol Res Ctr Biopesticide &, Changsha 410128, Peoples R China.;[Li, Zhongcai] Agr Bur Hanshou Cty, Plant Protect & Inspect Stn, Hanshou 415900, Peoples R China.

通讯机构： [Li, Youzhi] H;Hunan Agr Univ, Coll Plant Protect, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha 410128, Peoples R China.;Hunan Agr Univ, Hunan Prov Engn & Technol Res Ctr Biopesticide &, Changsha 410128, Peoples R China.

关键词： basic helix loop helix transcription factor;Drosophila protein;insect protein;MET protein, Drosophila;amino acid sequence;animal;female;gene knockdown;genetics;growth, development and aging;Hemiptera;insect control;metabolism;ovary;Amino Acid Sequence;Animals;Basic Helix-Loop-Helix Transcription Factors;Drosophila Proteins;Female;Gene Knockdown Techniques;Hemiptera;Insect Control;Insect Proteins;Ovary

摘要： Juvenile hormone (JH) is responsible for repressing larval metamorphosis and inducing vitellogenesis and egg production in insects. Methoprene-tolerant (Met) is known to be an intracellular receptor and transducer of JH. We examined the role of Met in ovarian development in the rice pest Sogatella furcifera (Horváth). We first cloned and sequenced S. furcifera Met (SfMet). The SfMet protein belongs to the basic helix-loop-helix/Per-Arnt-Sim (bHLH-PAS) family with a bHLH domain and two PAS domains (PAS-A and PAS-B). SfMet was expressed in all developmental stages and tissues but was most highly expressed in the ovaries of adult females. Furthermore, RNA interference (RNAi) mediated silencing of SfMet substantially reduced the expression of SfVg, decreased yolk protein deposition and blocked oocyte maturation and ovarian development. These results demonstrate that SfMet plays a key role in female reproduction in S. furcifera and suggest that targeting this gene could be an effective way of controlling this pest. © The Author(s) 2019.

语种： 英文

作者： Hu, Kui;Qiu, Lin;Zhang, Yurong;Du, Yu;He, Hualiang;...

期刊： JOURNAL OF ECONOMIC ENTOMOLOGY,2019年112(4):1541-1545 ISSN：0022-0493

通讯作者： Li, Youzhi

作者机构： [Hu, Kui; Qiu, Lin; Zhang, Yurong; Du, Yu; He, Hualiang; Li, Youzhi] Hunan Agr Univ, Coll Plant Protect, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha 410128, Hunan, Peoples R China;[Ding, Wenbing] Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Hunan, Peoples R China;[Ding, Wenbing; Li, Youzhi] Hunan Agr Univ, Hunan Prov Engn & Technol Res Ctr Biopesticide &, Changsha 410128, Hunan, Peoples R China

通讯机构： [Li, Youzhi] H;Hunan Agr Univ, Coll Plant Protect, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha 410128, Hunan, Peoples R China. Hunan Agr Univ, Hunan Prov Engn & Technol Res Ctr Biopesticide &, Changsha 410128, Hunan, Peoples R China.

关键词： animal;Hemiptera;insect vector;microinjection;Oryza;plant disease;Reoviridae;Animals;Hemiptera;Insect Vectors;Microinjections;Oryza;Plant Diseases;Reoviridae

摘要： The southern rice black-streaked dwarf virus (SRBSDV) causes significant economic damage to rice crops. This virus is transmitted to rice plants by the planthopper Sogatella furcifera (Horváth) in a persistent, circular, and propagative manner. Researchers currently lack suitable methods for assaying the activity of SRBSDV in vitro and preserving the virus all year. We used a microinjection method to directly inject SRBSDV extracts into the hemocoel of S. furcifera nymphs. SRBSDV was subsequently detected by Reverse Transcription-Polymerase Chain Reaction in more than 56.7% of the insects after 5 d and 60% of healthy rice plants fed by these insects also became SRBSDV infected. Moreover, injecting planthopper with an extract of SRBSDV-infected rice plant that had been frozen at -80°C for 220 d caused 63.3% to become viruliferous. These results indicate that SRBSDV can be successfully transmitted to S. furcifera by microinjection, and that extracts of SRBSDV-infected rice plants frozen at -80°C for 220 d still contain sufficient active SRBSDV to infect S. furcifera. We provide a novel way to preserve SRBSDV all year by injecting S. furcifera with the SRBSDV extract. © 2019 The Author(s). Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved.

语种： 英文

作者： 孙进军;唐涛;曹杨;李冠华;李有志;...

期刊： 湖南农业科学,2018年(8):56-60 ISSN：1006-060X

作者机构： 湖南农业大学植物保护学院;[唐涛] 湖南省植物保护研究所;[曹杨] 长沙市白蚁防治站;[姚振威] 仲恺农业工程学院;[孙进军; 李有志; 李冠华] 湖南农业大学

关键词： 直播水稻田;杂草;氯氟吡啶酯;五氟磺草胺;氰氟草酯;相对防效;安全性

摘要： 为了明确氯氟吡啶酯及其复配剂对直播稻田杂草的防效和对水稻的安全性,于2016年以H优518为供试水稻品种开展晚稻直播田杂草防除试验。结果表明：播后13 d喷施25 g/L氯氟吡啶酯乳油30～37.5 g/hm^2能有效防除稗草、鸭舌草、陌上菜和异型莎草;喷施32.5 g/L氯氟吡啶酯·五氟磺草胺油悬浮剂48.75～58.5 g/hm^2能有效防除稗草、鸭舌草、陌上菜、异型莎草和牛毛毡;喷施120 g/L氯氟吡啶酯·氰氟草酯乳油144～216 g/hm^2能有效防除稗草、鸭舌草、陌上菜、异型莎草和千金子。常用药剂25 g/L五氟磺草胺油悬浮剂37.5 g/hm^2能有效防除稗草、鸭舌草、陌上菜、异型莎草和牛毛毡;10%噁唑酰草胺乳油与10%氰氟草酯乳油混用150＋150 g/hm^2可有效防除稗草和千金子;10%吡嘧磺隆可湿性粉剂与10%双草醚悬浮剂混用45＋45 g/hm^2可有效防除稗草、鸭舌草、陌上菜、异型莎草和牛毛毡。各药剂均对水稻安全。

语种： 中文

作者： Qiu, Lin;Tao, Shunjie;He, Hualiang;Ding, Wenbing;Li, Youzhi*

期刊： BMC Genomics,2018年19(1):1-12 ISSN：1471-2164

通讯作者： Li, Youzhi

作者机构： [Qiu, Lin; Li, Youzhi; Tao, Shunjie; Ding, Wenbing; He, Hualiang] Hunan Agr Univ, Coll Plant Protect, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha 410128, Hunan, Peoples R China.;[Li, Youzhi; Ding, Wenbing] Hunan Prov Engn & Technol Res Ctr Biopesticide &, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Li, Youzhi] H;Hunan Agr Univ, Coll Plant Protect, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha 410128, Hunan, Peoples R China.;Hunan Prov Engn & Technol Res Ctr Biopesticide &, Changsha 410128, Hunan, Peoples R China.

关键词： Chlorops oryzae;Transcriptome;Chemosensory genes;Olfactory receptors;Expression profiling

摘要： Background: Chemosensory proteins are a family of insect-specific chemical sensors that sense specific chemical cues and regulate insect behavior. Chemosensory proteins have been identified and analyzed in many insect species, such as Drosophila melanogaster, Bactrocera dorsalis and Calliphora stygia. This research has revealed that these proteins play a crucial role in insect orientation, predation and oviposition. However, little is known about the chemosensory proteins of Chlorops oryzae, a major pest of rice crops throughout Asia. Results: Comparative transcription analysis of the genes of Chlorops oryzae larvae, pupae and adults identified a total of 104 chemosensory genes, including 25 odorant receptors (ORs), 26 odorant-binding proteins (OBPs), 19 ionotropic receptors (IRs), 23 gustatory receptors (GRs) and 11 sensory neuron membrane proteins (SNMPs). The sequences of these candidate chemosensory genes were confirmed and used to construct phylogenetic trees. Quantitative real-time PCR (qRT-PCR) confirmed that the expression of candidate OR genes in different developmental stages was consistent with the fragments per kilobase per million fragments (FPKM) values of differentially expressed genes (DEGs). Conclusions: The identification of chemosensory genes in C. oryzae provides a foundation for the investigation of the function of chemosensory proteins in this species, which, in turn, could allow the development of new, improved methods of controlling this pest. © 2018 The Author(s).

语种： 英文

作者： 贺华良;胡岩;叶波;张龙杰;雷振东;...

期刊： 湖南农业大学学报(自然科学版),2018年44(6):613-619 ISSN：1007-1032

作者机构： 湖南农业大学植物保护学院, 湖南, 长沙, 410128;安乡县农业局, 湖南, 安乡, 415600;怀化市农业局, 湖南, 怀化, 418000;湖南省植保植检站, 湖南, 长沙, 410005;[贺华良; 胡岩; 丁文兵; 李有志] 湖南农业大学植物保护学院, 湖南, 长沙, 410128

关键词： 稻水象甲;简单重复序列间区;遗传多样性;扩散;湖南

摘要： 为全面了解湖南省稻水象甲的入侵扩散特点,运用简单重复序列间区(ISSR)分子标记方法及对比COⅠ基因、ITS2片段的序列信息,探讨全省17个地理种群的遗传多样性和扩散模式。结果表明:湖南省稻水象甲种群COⅠ基因、ITS2片段的序列非常保守,不能提供有效的多态性位点信息,而ISSR的多态性信息非常丰富,其多态性位点百分率可达93.24%;基于ISSR的数据分析发现,湖南省稻水象甲的Nei’s基因多样性指数为0.363 0,各地理种群间基因分化系数为0.414 3,种群之间的遗传距离与地理距离无相关性。湖南省稻水象甲地理种群分化不符合地理隔离模式,以人为因素导致的扩散为主,自然扩散为辅。

语种： 中文

作者： 申晓娜;吕志创;李有志;刘万学;万方浩

期刊： 植物保护,2018年44(1):52-58 ISSN：0529-1542

作者机构： 湖南农业大学植物保护学院, 长沙, 410128;中国农业科学院植物保护研究所, 植物病虫害生物学国家重点研究室, 北京, 100193;[申晓娜] 湖南农业大学植物保护学院, 长沙, 410128 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点研究室, 北京, 100193;[吕志创; 刘万学; 万方浩] 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点研究室, 北京, 100193;[李有志] 湖南农业大学植物保护学院, 长沙, 410128

关键词： 烟粉虱;温度胁迫;抗寒基因;定量表达

摘要： 烟粉虱Bemisia tabaci具有强的温度耐受性,但烟粉虱入侵种MED隐种与本地种亚洲Ⅱ3隐种在我国的地理分布上存在巨大的差异。因此本研究以烟粉虱MED隐种和亚洲Ⅱ3隐种为研究对象,采用qRT-PCR技术分别检测两种烟粉虱3个抗寒基因(erythrocyte binding protein,trehalase,dopadecarboxylase)在5个梯度温度胁迫下mRNA水平上的相对表达量,分析其在高低温胁迫过程中表达量的变化,探索抗寒基因在烟粉虱MED隐种成功入侵并广泛分布于各气候区域中所起的作用。研究结果表明这两种烟粉虱隐种体内,3个抗寒基因均显著受低温诱导。但相对于AsiaⅡ3隐种,低温胁迫下烟粉虱MED隐种体内的3种抗寒基因能更快速地做出响应,且基因上调或下调的量也相对较多。

语种： 中文

作者： Liu, Binglei;Ding, Wenbing;Huang, Shuying;Sun, Weihua;Li, Youzhi*

期刊： Biochemical Systematics and Ecology,2018年78:35-38 ISSN：0305-1978

通讯作者： Li, Youzhi

作者机构： [Li, Youzhi; Ding, Wenbing; Sun, Weihua; Huang, Shuying; Liu, Binglei] Hunan Agr Univ, Coll Plant Protect, Hunan Prov Engn & Technol Res Ctr Biopesticide &, Changsha 410128, Hunan, Peoples R China.;[Li, Youzhi; Ding, Wenbing] Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Hunan, Peoples R China.;[Li, Youzhi] Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Li, Youzhi] H;Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.

关键词： Benzofuran;Lignan;Styrax Linn;Styrax suberifolius

摘要： The first phytochemical investigation of the bark of Styrax suberifolius Hook. Et Arn. (Styracaceae) successfully isolated and identified seventeen phenylpropanoids, including ten benzofuran derivatives (1-10), two ditetrahydrofurans (11 and 12), two neolignans (13 and 14) and three phenyl alkanols (15-17). Compounds 2-(3-hydroxy-4-methoxyphenyl)-7-methoxy-5-benzofuranpropa- nol (5), (+)-cedrusin (6), ( - )-(7R,8S)-dihydrodehydrodiconiferyl alcohol 4-O-beta-D- glucopyranoside (8), ( - )-(7S,8R)-dihydrodehydrodiconiferyl alcohol 4-O-beta-D- glucopyranoside (9), ( +)-(7S,8R)-dihydrodehydrodiconiferyl alcohol (10), ( +)-(7S,8R) -erythro-4,7,9,9'tetrahydroxy-3,3'-dimethoxy-8-O-4'-neolignan (13), ( - )-symplocos- neolignan A (14), ( - )-7-O-ethylguaiacylglycerol (15), 2-[4-(3-hydroxypropyl)-2- methoxyphenoxy]-1,3-propanediol (16) and dihydroconiferyl alcohol (17) have not been recorded in Styrax Linn. previously. The chemotaxonomic significance of isolated compounds is discussed.

语种： 英文