作者： Shi, Li*;Shi, Yao;Liu, Meng-Fei;Zhang, Ya;Liao, Xiao-Lan*

期刊： 昆虫科学(英文),2021年28(5):1426-1438 ISSN：1672-9609

通讯作者： Shi, Li;Liao, Xiao-Lan

作者机构： [Liu, Meng-Fei; Zhang, Ya; Liao, Xiao-Lan; Shi, Li; Shi, Yao] Hunan Agr Univ, Hunan Prov Engn & Technol Res Ctr Biopesticide &, Coll Plant Protect, Changsha, Peoples R China.;[Liu, Meng-Fei; Zhang, Ya; Liao, Xiao-Lan; Shi, Li; Shi, Yao] Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha, Peoples R China.;[Shi, Li; Liao, XL] Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Peoples R China.

通讯机构： [Shi, L; Liao, XL] H;Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Peoples R China.

关键词： Spodoptera litura;indoxacarb resistance;transcription factor;detoxification genes;RNA interference;RNA sequencing

摘要： <jats:title>Abstract</jats:title><jats:p>The xenobiotic transcription factor cap ‘n’ collar isoform C (CncC) is considered the central regulator of antioxidant and detoxification genes. Previous research indicated that CncC regulates three‐phase enzymes responsible for insecticide resistance. In this study, the <jats:italic>SlituCncC</jats:italic> gene from <jats:italic>Spodoptera litura</jats:italic> was obtained and characterized. Quantitative polymerase chain reaction (qPCR) analysis showed that <jats:italic>SlituCncC</jats:italic> was expressed in all developmental stages and tissues, but was highly expressed in 3rd‐ and 4th‐instar larvae, and in the Malpighian tubule, fat body, and midgut. In addition, <jats:italic>SlituCncC</jats:italic> was up‐regulated and more highly induced with indoxacarb treatment in the indoxacarb‐resistant strains compared with the susceptible strain. RNA interference‐mediated gene silencing of <jats:italic>SlituCncC</jats:italic> significantly increased mortality of <jats:italic>S.litura</jats:italic> when exposed to indoxacarb. Furthermore, comparative transcriptome analysis showed that 842 genes were down‐regulated and 127 genes were up‐regulated in <jats:italic>SlituCncC</jats:italic> knockdown <jats:italic>S. litura</jats:italic>. Further analysis indicated that 18 three‐phase enzymes were identified in the down‐regulated genes, of which seven were associated with indoxacarb resistance in <jats:italic>S. litura</jats:italic>. qPCR analysis confirmed that expression of six of these seven genes was consistent with RNA sequencing data. All six detoxification genes were induced by indoxacarb, and the expression patterns were similar to that of <jats:italic>SlituCncC</jats:italic>. Finally, the CncC–Maf binding site was predicted in all six gene promoters. This study indicates that the transcription factor <jats:italic>SlituCncC</jats:italic> may regulate multiple detoxification genes that mediate indoxacarb resistance in <jats:italic>S. litura</jats:italic>.</jats:p>

语种： 英文

作者： Peng, Yajun;Cheng, Xiaomei;Liu, Ducai;Liu, Xueyuan;Ma, Guolan;...

期刊： Crop Protection,2021年141:105501 ISSN：0261-2194

通讯作者： Bai, Lianyang;Zhang, Yuping

作者机构： [Peng, Yajun; Bai, Lianyang] Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Peoples R China.;[Zhang, Yuping] Henan Inst Sci & Technol, Xinxiang 453003, Henan, Peoples R China.;[Li, Sifu; Liu, Xueyuan; Liu, Ducai; Cheng, Xiaomei; Peng, Yajun; Ma, Guolan; Bai, Lianyang] Hunan Acad Agr Sci, Changsha 410125, Peoples R China.;[Yang, Yuting] Dingyuan Jiahe Crop Protect Co Ltd, Dingyuan 233200, Peoples R China.

通讯机构： [Bai, Lianyang; Zhang, Yuping] H;Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Peoples R China.;Henan Inst Sci & Technol, Xinxiang 453003, Henan, Peoples R China.

关键词： Paddy rice;Quintrione;Rice yield;Weed

摘要： Weeds present a serious problem in rice paddy fields and severely affect rice (Oryza sativa L.) quality and yield. Quintrione is a new herbicide developed for use in rice. It inhibits p-hydroxyphenylpyruvate dioxygenase and regulates hormone levels to control weeds. In 2014–2016, field plot trials were conducted to evaluate the control efficacies of quintrione at different application rates and times against barnyardgrass, Echinochloa crus-galli (L.) P.Beauv., climbing seedbox, Ludwigia prostrata Roxb., and sheathed monochoria, Monochoria vaginalis (Burm. f.) C.Presl. Its phytotoxic effects on rice were compared with those of pretilachlor, penoxsulam and metamifop in Changsha, China. Quintrione, under the tested conditions, caused various levels of phytotoxicity in rice when applied 3 days after sowing (DAS) only. However, greater than 90% control efficacies against barnyardgrass were achieved by quintrione at 300 g a.i. ha−1 at 3 DAS, 450 g a.i. ha−1 at early post-emergence (EPOST) and 600 g a.i. ha−1 at early tillering (ET), against climbing seedbox at all the tested application rates and times, and against sheathed monochoria at 3 DAS only. Quintrione had a greater control efficacy against climbing seedbox and a similar efficacy against barnyardgrass compared with pretilachlor, penoxsulam and metamifop. Quintrione showed significantly greater, similar and lower efficacies against sheathed monochoria than metamifop, pretilachlor and penoxsulam, respectively. In addition, the rice yields after quintrione treatments were greater than, or similar to, those after pretilachlor, penoxsulam and metamifop treatments. Thus, quintrione appears to be an effective herbicide to control barnyardgrass and climbing seedbox in rice paddies when used at EPOST and ET stages. © 2020 Elsevier Ltd

语种： 英文

作者： Zheng, H.;Xie, W.*;Fu, B.;Xiao, S.;Tan, X.;...

期刊： Pest Management Science,2021年77(6):2990-3001 ISSN：1526-498X

通讯作者： Xie, W.;Zhang, Y.

作者机构： [Xie, W; Zhang, Y.; Xiao, S.; Wang, S.; Xie, W.; Ji, Y.; Yang, X.; Xu, B.; Wu, Q.; Fu, B.; Zheng, H.; Guo, Z.; Cheng, J.; Tan, X.] Chinese Acad Agr Sci, Dept Plant Protect, Inst Vegetables & Flowers, Beijing 100081, Peoples R China.;[Zheng, H.] Hunan Agr Univ, Coll Plant Protect, Changsha, Peoples R China.;[Fu, B.] Chinese Acad Trop Agr Sci, Minist Agr & Rural Affairs, Key Lab Integrated Pest Management Trop Crops, Environm & Plant Protect Inst, Haikou, Hainan, Peoples R China.;[Wang, R.] Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing, Peoples R China.;[Liu, B.] Tianjin Acad Agr Sci, Inst Plant Protect, Tianjin, Peoples R China.

通讯机构： [Xie, W; Zhang, Y] C;Chinese Acad Agr Sci, Dept Plant Protect, Inst Vegetables & Flowers, Beijing 100081, Peoples R China.

关键词： Bemisia tabaci;resistance;insecticides;spirotetramat;cyantraniliprole

摘要： BACKGROUND: Over recent decades, many efficacious insecticides have been applied for control of Bemisia tabaci, one of the most notorious insect pests worldwide. Field-evolved insecticide resistance in B. tabaci has developed globally, but remains poorly understood in China. RESULTS: In this study, a total of 30 field samples of the whitefly Bemisia tabaci from eight provinces of China were collected in 2015 to 2018. Twenty-four of the populations were identified as Mediterranean, ‘Q’ type (MED), three were Middle East-Asia Minor 1, ‘B’ type (MEAM1), and three were mixtures of MED/ MEAM1. After identifying whether they belong to MED or MEAM1, the selected individuals were used in bioassays assessing insecticide resistance to abamectin, thiamethoxam, spirotetramat, cyantraniliprole, and pyriproxyfen. Our results showed that all populations in the eight regions had little or no resistance to abamectin; abamectin resistance was highest in the Hunan (Changsha) and Hubei (Wuhan) regions and was lowest in the island region of Hainan (Sanya). The resistance of B. tabaci to spirotetramat, cyantraniliprole, and pyriproxyfen increased each year. The resistance to thiamethoxam remained low because of the high LC50 value for the laboratory strain. CONCLUSION: These findings suggest that a rotation system using efficacious B. tabaci insecticides with differing mode of actions ought to be implemented for sustainable control to reduce the potential of resistance development. This study provides important data to support the integrated pest management and insecticide resistance management of B. tabaci in China. © 2021 Society of Chemical Industry. © 2021 Society of Chemical Industry.

语种： 英文

作者： Wei, W.;Toe, T.;Ya, Z.;Ke-Cheng, Z.;Ming-Guo, J.;...

期刊： 农业资源与环境学报,2020年37(3):407-412 ISSN：2095-6819

通讯作者： Bei-Bei, G.

作者机构： [Wei, W.; Ke-Cheng, Z.; Wei, Z.; Li-Ming, S.; Bei-Bei, G.] Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing, 100193, China;[Ya, Z.] College of Plant Protection, Hunan Ag⁃ ricultural University, Changsha, 410128, China;[Ming-Guo, J.] Guangxi University for Nationalities, Nanning, 530006, China;Biotechnology Research Center, Department of Research and Innovation, Kyaukse, 05152, Myanmar;[Toe, T.] College of Plant Protection, Hunan Ag⁃ ricultural University, Changsha, 410128, China<&wdkj&>Biotechnology Research Center, Department of Research and Innovation, Kyaukse, 05152, Myanmar

通讯机构： Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing, China

关键词： 植物生长调节剂;分子鉴定;生物测定;肠杆菌;番茄

摘要： 为筛选出一株具有微生物源植物调节剂功能的细菌,并探讨其潜在的生物功能,利用平板涂布法以及16S rRNA鉴定技术对菌株进行分离、鉴定;同时使用该菌株发酵液对番茄的种子和幼苗分别进行浸种和灌根处理,并进行农艺指标测定。结果表明:经鉴定该菌株为烟草肠杆菌,其发酵液对番茄种子的萌发、幼苗的生长具有显著的促进作用;发酵液浓度为1×104cfu·mL-1时促生效果最好,与对照相比,根长、株高、干质量和鲜质量分别增加63.42%、46.17%、150.00%和144.83%。研究结果可为该菌株开发为微生物肥料或微生物源植物生长调节剂提供技术支持。

语种： 中文

作者： Liu, Shaonan;He, Chao;Liang, Jinjin;Su, Qi;Hua, Dengke;...

期刊： Pesticide Biochemistry and Physiology,2020年167:104602 ISSN：0048-3575

通讯作者： Zhang, Youjun

作者机构： [Liu, Shaonan; Hua, Dengke; Su, Qi] Yangtze Univ, Coll Agr, Hubei Engn Technol Ctr Pest Forewarning & Managem, Jingzhou 434025, Hubei, Peoples R China.;[Xie, Wen; Zhang, Youjun; Wang, Shaoli; He, Chao; Wu, Qingjun] Chinese Acad Agr Sci, Inst Vegetables & Flowers, Dept Plant Protect, Beijing 100081, Peoples R China.;[Liang, Jinjin] Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Peoples R China.

通讯机构： [Zhang, Youjun] C;Chinese Acad Agr Sci, Inst Vegetables & Flowers, Dept Plant Protect, Beijing 100081, Peoples R China.

关键词： cytochrome P450;insect protein;insecticide;animal;Hemiptera;insect;phylogeny;Animals;Cytochrome P-450 Enzyme System;Hemiptera;Insect Proteins;Insecta;Insecticides;Phylogeny

摘要： The ecdysteroid hormone 20-hydroxyecdysone (20E), a critical hormone in arthropods, plays an essential role in insect growth, molting and reproduction. A previous study showed that 20E is actually regulated by six P450 genes (five P450 genes belonging to the Halloween family and a CYP18A1 gene) in model insects. However, the role of the six P450 genes in Bemisia tabaci Q (also call Mediterranean, MED), an important pest of field crops, remains unclear. Here, six P450 genes were cloned by RT-PCR, and the phylogenetic tree indicated a close orthologous relationship of these P450 genes between MED and other insects. Spatiotemporal expression profiling revealed that five P450 genes (CYP18A1, CYP306A1, CYP307A2, CYP314A1 and CYP315A1) were expressed at significantly higher levels in the head than in the abdomen and thorax. Four P450 genes (CYP302A1, CYP307A2, CYP314A1 and CYP315A1) were expressed at the highest levels in males, and CYP18A1 was expressed at the highest levels in the 4th nymph stage. The molting process was delayed by approximately 1–3 days after knockdown of these genes at the 4th nymph stage, and the mean proportion of shriveled or dead insects reached 8.3% (CYP18A1), 20.8% (CYP302A1), 7.0% (CYP307A2), 31.8% (CYP306A1), 28.6% (CYP314A1) and 24.1% (CYP315A1). In addition, 20E rescued the negative effect of ds-CYP306A1, ds-CYP314A1 and ds-CYP315A1 on the eclosion rate. We concluded that these Halloween genes and CYP18A1 likely participate in the development of MED, and in particular, CYP306A1 could be used as a putative insecticide target for controlling this piercing-sucking insect. © 2020 Elsevier Inc.

语种： 英文

作者： Liu, Yiping;Liu, Shuangqing;Zhang, Ya;Qin, Dongmei;Zheng, Zuntao;...

期刊： Food Control,2020年107:106754 ISSN：0956-7135

通讯作者： Liao, Xiaolan;Li, Xiaogang

作者机构： [Zhang, Ya; Liu, Shuangqing; Li, Xiaogang; Liao, Xiaolan; Liu, Yiping] Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.;[Zheng, Zuntao; Qin, Dongmei; Zhu, Guangyan] Minist Agr & Rural Affairs, Inst Control Agrochem, Beijing 100125, Peoples R China.;[Liu, Zhaoqing; Lv, Yuntao; Liu, Yiping] Hunan Prov Dept Agr & Rural Affairs, Hunan Prov Inst Control Agrochem, Changsha 410005, Hunan, Peoples R China.;[Dong, Zhe] Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Beijing 100085, Peoples R China.;[Liao, Xiaolan; Li, XG] 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Liao, XL; Li, XG] 1;1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.

关键词： Degradation kinetics;Dietary risk assessment;Gas chromatography;Malathion;Maximum residual limits

摘要： The gas chromatography-flame photometric detector (GC-FPD) was used to detect malathion residues content in vegetables (cherry tomatoes and broccoli) and fruits (mulberries, cranberries, and figs). The recoveries of samples were 76.2 ± 3.5%–103.9 ± 3.6% at a spike level of 0.01–10 mg/kg, the relative standard deviation (RSDs) were 2.1%–7.3%, and the limit of quantification (LOQs) were 0.01–0.02 mg/kg. On the basis of the normative field trials, the degradation half-life of malathion were 1.10–2.15 days, which indicated that malathion degrades quickly. The final distribution of malathion residues in various vegetables and fruits varied from <0.01 to 0.58 mg/kg. The long-term dietary risk assessment results showed that the national estimated daily intake (NEDI) of malathion in various populations of China ranged from 0.000021 to 0.000549 mg/kg bw, and the risk quotient (RQ) ranged from 0.007% to 0.183% and were less than 100%, which indicated that malathion has a low risk for long-term dietary intake these crops. The maximum residual limits (MRLs) of malathion can be set as 1 mg/kg for cherry tomatoes, broccoli, mulberries, cranberries, and 0.2 mg/kg for figs, respectively. These results would be considered as important references for monitoring and assessing the quality safety of agricultural products and protecting consumer health. © 2019 Elsevier Ltd

语种： 英文

作者： Yang, Fengbo;Zhang, Qinghe;Yao, Qixi;Chen, Gong;Tong, Hong;...

期刊： Journal of Pest Science,2020年93(4):1243-1254 ISSN：1612-4758

通讯作者： Su, Qi;Zhang, Youjun

作者机构： [Zhang, Qinghe; Zhang, Jianmin; Yang, Fengbo; Su, Qi; Yao, Qixi; Tong, Hong; Li, Chuanren] Yangtze Univ, Coll Agr, Hubei Engn Technol Ctr Pest Forewarning & Managem, Jingzhou 434025, Hubei, Peoples R China.;[Chen, Gong; Zhang, Youjun] Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China.;[Chen, Gong] Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Su, Qi] Y;[Zhang, Youjun] C;Yangtze Univ, Coll Agr, Hubei Engn Technol Ctr Pest Forewarning & Managem, Jingzhou 434025, Hubei, Peoples R China.;Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China.

关键词： biological control;crop plant;cultivar;fruit;ingestion rate;pest control;plant defense;whitefly;Aleyrodidae;Bemisia tabaci;Encarsia formosa;Hexapoda;Lycopersicon esculentum

摘要： Plants have evolved induced defense mechanisms against insect herbivore attacks. The application of plant elicitors can enhance the biological control of crop pests by inducing plant defense responses, and (Z)-3-hexenol (z3HOL) is recognized as a herbivore-induced plant volatile that plays an important role in the chemical communication between plants and pests. We investigated whether z3HOL can activate the defense mechanism of tomato (Solanum lycopersicum) against the whitefly Bemisia tabaci, one of the major insect pests of cultivated tomato. Exposure to z3HOL vapors in the glass jars decreased weight gain and oviposition in B. tabaci and negatively influenced the feeding behavior of B. tabaci by shortening the total feeding period and phloem ingestion and increasing the frequency of stylet puncture. Furthermore, exposure to z3HOL induced jasmonic acid- and salicylic acid-mediated defense responses and enhanced B. tabaci-induced volatile emissions in tomato. The elevated release of tomato volatiles increased the attraction of the parasitoid Encarsia formosa and improved its parasitism on B. tabaci. Our results indicate that z3HOL can serve as an elicitor that triggers direct and indirect defense responses against B. tabaci by modulating signaling pathways in tomato. These findings will not only help us to gain a better understanding of how z3HOL influences the crop community, but also provide new insights into the potential application of z3HOL in integrated pest management systems. © 2020, Springer-Verlag GmbH Germany, part of Springer Nature.

语种： 英文

作者： Feng, Kaiyang;Ou, Shiyuan;Zhang, Ping;Wen, Xiang;Shi, Li;...

期刊： Pest Management Science,2020年76(2):665-675 ISSN：1526-498X

通讯作者： He, Lin

作者机构： [Feng, Kaiyang; Xu, Zhifeng; He, Lin; Wen, Xiang; Ou, Shiyuan; Hu, Yuan; Yang, Yuwei; Shen, Guangmao; Zhang, Ping] Southwest Univ, Coll Plant Protect, Key Lab Entomol & Pest Control Engn, Chongqing 400716, Peoples R China.;[Feng, Kaiyang; Xu, Zhifeng; He, Lin; Wen, Xiang; Ou, Shiyuan; Hu, Yuan; Yang, Yuwei; Shen, Guangmao; Zhang, Ping] Southwest Univ, Acad Agr Sci, Chongqing 400716, Peoples R China.;[Shi, Li] Hunan Agr Univ, Coll Plant Protect, Changsha, Hunan, Peoples R China.;[Zhang, Yichao] Shanxi Univ, Res Inst Appl Biol, Taiyuan, Shanxi, Peoples R China.;[He, Lin] Southwest Univ, Key Lab Entomol & Pest Control Engn, Coll Plant Protect, Acad Agr Sci, Chongqing 400716, Peoples R China.

通讯机构： [He, Lin] S;Southwest Univ, Key Lab Entomol & Pest Control Engn, Coll Plant Protect, Acad Agr Sci, Chongqing 400716, Peoples R China.

关键词： cytochrome;gene expression;genetic engineering;inhibitor;metabolite;mite;pest resistance;physiological response;Tetranychidae;Tetranychus cinnabarinus;acaricide;arthropod protein;cyflumetofen;cytochrome P450;propionic acid derivative;animal;drug resistance;tandem mass spectrometry;Tetranychidae;Acaricides;Animals;Arthropod Proteins;Cytochrome P-450 Enzyme System;Drug Resistance;Propionates;Tandem Mass Spectrometry;Tetranychidae

摘要： BACKGROUND: Acaricide resistance is a serious problem in spider mites. Cyflumetofen is a new complex II inhibitor, whereas pyridaben acts at complex I and has been used for decades. Although cross-resistance between cyflumetofen and pyridaben has been observed in Tetranychus cinnabarinus, the specific mechanisms at play have not yet been investigated. RESULTS: Investigation into the cross-resistance mechanisms identified five P450s, among which CYP389C16 was evaluated as the most likely candidate conferring cross-resistance. Knockdown of CYP389C16 expression via RNA interference diminished the level of cross-resistance in the cyflumetofen-resistant strain. In addition, recombinant CYP389C16 (40 pmol) effectively metabolized 25.0 ± 0.7% of cyflumetofen, 39.7 ± 1.0% of pyridaben, and 69.3 ± 3.3% of AB-1 (active de-esterified metabolite of cyflumetofen) within 2 h. In addition, hydroxylation metabolite of AB-1 was identified by HPLC-MS/MS. CONCLUSIONS: The study reveals that overexpressed CYP389C16 is involved in the cross-resistance between cyflumetofen and pyridaben in T. cinnabarinus. © 2019 Society of Chemical Industry. © 2019 Society of Chemical Industry

语种： 英文

作者： He, Chao;Liang, Jinjin;Liu, Shaonan;Zeng, Yang;Wang, Shaoli;...

期刊： Pesticide Biochemistry and Physiology,2020年162:29-35 ISSN：0048-3575

通讯作者： Xie, Wen;Zhang, Youjun

作者机构： [Liu, Shaonan; Xie, Wen; Zhang, Youjun; Wang, Shaoli; He, Chao; Wu, Qingjun; Xie, W; Zhang, YJ; Zeng, Yang] Chinese Acad Agr Sci, Inst Vegetables & Flowers, Dept Plant Protect, Beijing 100081, Peoples R China.;[Liang, Jinjin] Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Peoples R China.

通讯机构： [Xie, W; Zhang, YJ] C;Chinese Acad Agr Sci, Inst Vegetables & Flowers, Dept Plant Protect, Beijing 100081, Peoples R China.

关键词： cytochrome P450;imidacloprid;imidazole derivative;insecticide;neonicotinoid;nitro derivative;reduced nicotinamide adenine dinucleotide phosphate ferrihemoprotein reductase;animal;Hemiptera;insecticide resistance;male;Animals;Cytochrome P-450 Enzyme System;Hemiptera;Imidazoles;Insecticide Resistance;Insecticides;Male;NADPH-Ferrihemoprotein Reductase;Neonicotinoids;Nitro Compounds

摘要： NADPH cytochrome P450 reductase (CPR) is an integral component of cytochrome P450-mediated biological reactions, such as the metabolism of xenobiotics, including insecticides. CPR has been reported to be associated with insecticide tolerance in several insects. However, the biochemical characteristics and biological function of CPR in Bemisia tabaci Q (BtCPR) remain undefined. In this study, BtCPR was cloned, and bioinformatic analysis showed that BtCPR is a transmembrane protein with a molecular weight (MW) of 76.73 kDa and contains conserved binding domains (FMN, FAD, and NADPH). Tissue- and developmental stage-dependent expression indicated that the highest expression levels of BtCPR occurred in head tissue and in male adults. Transcripts of BtCPR in the field B. tabaci Q strain were 1.62-fold higher than those of the laboratory B. tabaci Q strain. In both field and laboratory adults, the susceptibility of BtCPR-knockdown B. tabaci Q to imidacloprid substantially increased compared to that of the B. tabaci Q control group. Furthermore, the heterologous expression of BtCPR in Sf9 cells exhibited catalytic activity for cytochrome c reduction, following Michaelis-Menten kinetics. Sf9 cells overexpressing BtCPR had greater viability than the control cells when treated with imidacloprid. The results suggest that BtCPR could affect the susceptibility of B. tabaci Q to imidacloprid and could also be considered a novel target for pest control. © 2019 Elsevier Inc.

语种： 英文

作者： Wei, Peng;Li, Jinhang;Liu, Xinyang;Nan, Can;Shi, Li;...

期刊： Pest Management Science,2019年75(1):252-261 ISSN：1526-498X

通讯作者： He, Lin

作者机构： [Li, Jinhang; He, Lin; Zhang, Yichao; Wei, Peng; Liu, Xinyang; Nan, Can; Li, Chuanzhen; Shi, Li] Southwest Univ, Acad Agr Sci, Chongqing 400716, Peoples R China.;[Li, Jinhang; He, Lin; Zhang, Yichao; Wei, Peng; Liu, Xinyang; Nan, Can; Li, Chuanzhen; Shi, Li] Southwest Univ, Coll Plant Protect, Chongqing 400716, Peoples R China.;[Shi, Li] Hunan Agr Univ, Coll Plant Protect, Changsha, Hunan, Peoples R China.

通讯机构： [He, Lin] S;Southwest Univ, Acad Agr Sci, Chongqing 400716, Peoples R China.;Southwest Univ, Coll Plant Protect, Chongqing 400716, Peoples R China.

关键词： Tetranychus;pyrethroid resistance;carboxylesterase;RNAi;gene expression

摘要： BACKGROUND: Carboxylesterases (CarEs) are important in pesticide resistance. Four overexpressed CarE genes with inducible character were screened out in fenpropathrin-resistant Tetranychus cinnabarinus, but their functional roles remained to be further analyzed by RNAi and protein expression. RESULTS: Feeding a single double-stranded (ds)RNA of each of four genes led to gene-specific downregulation of mRNA, decreased esterase activity and diminished resistance in T. cinnabarinus. More interestingly, feeding four dsRNAs simultaneously led to a more significant decrease in enzymatic activity and fold resistance than feeding a single dsRNA individually, suggesting that these CarE genes were involved in fenpropathrin-resistance and had cooperative roles. The gene CarE6 was regarded as the primary and representative candidate to be functionally expressed, because silencing of CarE6 led to the most significant decrease in resistance level. The activity of CarE6 protein was competitively inhibited by fenpropathrin. It could effectively decompose 41.7 ± 0.09% of fenpropathrin within 3 h, proving that CarE6 protein was capable of metabolizing fenpropathrin effectively in T. cinnabarinus. CONCLUSION: The results confirm that four CarE genes are cooperatively involved in fenpropathrin resistance and the metabolic enzymes encoded by these overexpressed genes do indeed metabolize acaricide in resistant T. cinnabarinus in the evolution of acaricide resistance. © 2018 Society of Chemical Industry. © 2018 Society of Chemical Industry

语种： 英文

作者： Zhang, Ya;Liu, Shuangqing*;Wang, Chong;Liao, Xiaolan*

期刊： International Journal of Agriculture and Biology,2019年22(6):1467-1475 ISSN：1560-8530

通讯作者： Liu, Shuangqing;Liao, Xiaolan

作者机构： [Zhang, Ya; Liu, Shuangqing; Liao, Xiaolan; Liao, XL] Hunan Agr Univ, Coll Plant Protect, Changsha, Hunan, Peoples R China.;[Liu, Shuangqing] Hunan Prov Key Lab Biol & Control Plant Dis & Pla, Changsha, Hunan, Peoples R China.;[Wang, Chong] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China.;[Liao, Xiaolan] Hunan Biol Pesticides & Pesticide Preparat Proc E, Changsha, Hunan, Peoples R China.

通讯机构： [Liu, SQ; Liao, XL; Liu, Shuangqing; Liao, Xiaolan] H;Hunan Agr Univ, Coll Plant Protect, Changsha, Hunan, Peoples R China.;Hunan Prov Key Lab Biol & Control Plant Dis & Pla, Changsha, Hunan, Peoples R China.;Hunan Biol Pesticides & Pesticide Preparat Proc E, Changsha, Hunan, Peoples R China.

关键词： Active components;Antagonistic bacteria;Botrytis cinerea;Control effect;Identification

摘要： To resolve the problem of pesticide resistance in strawberry gray mold Botrytis cinerea and to improve the effectiveness of disease preventive measures by developing biological pesticides, a bacterial strain was identified using plate confrontation culture method, morphological characteristics, physiological and biochemical analyses, and 16S rDNA gene sequence analysis. The antifungal components of the antagonistic bacterial strain were analyzed by GC-MS, and their in vitro control effects were evaluated. One hundred bacterial strains were isolated from the strawberry rhizosphere soil, of which one showed superior antagonistic effect, accounting for 1% of all of the strains, and it was designated as strain ZY86. Further investigation indicated that strain ZY86 exhibited antifungal effects not only against B. cinerea but also against Rhizoctonia solani, Magnaporthe grisea, Colletotrichum capsici, Phytophthora capsici and Sclerotinia sclerotiorum. Strain ZY86 (GenBank Accession number MK111627) was highly similar to model strain Acinetobacter johnsonii (ATCC17909), with a homologous identity of 99%, and thus, strain ZY86 was identified as A. johnsonii. Analyses of the antifungal components showed that the active components produced by strain ZY86 were primarily amines, accounting for 20.80% of the total active components, which were followed, in order, by ketones, acids, esters, alcohols, peptides, hydrocarbons, alkanes, aldehydes, and alkenes in terms of proportion. In vitro testing showed that the control effect of strain ZY86 on strawberry gray mold was 84.14%, and higher the concentration, the better the control effect. In addition, strain ZY86 imparted a strong control effect against strawberry gray mold, and therefore, it is a promising biocontrol agent for agricultural production. (C) 2019 Friends Science Publishers

语种： 英文

作者： Zhang, Yuguang;Zhong, Yuhong;Hong, Yanyun;Xue, Yadong;Li, Diqiang;...

期刊： Conservation Genetics,2019年20(4):817-824 ISSN：1566-0621

通讯作者： Zhou, Chengran;Liu, Shanlin

作者机构： [Zhang, Yuguang; Xue, Yadong; Li, Diqiang] Chinese Acad Forestry, Inst Forest Ecol Environm & Protect, Key Lab Biol Conservat, Natl Forestry & Grassland Adm, Beijing 100091, Peoples R China.;[Zhong, Yuhong; Zhou, Chengran; Liu, Shanlin; Zhou, CR; Liu, SL] BGI Shenzhen, Shenzhen 518083, Peoples R China.;[Zhong, Yuhong; Zhou, Chengran; Liu, Shanlin; Zhou, CR; Liu, SL] BGI Shenzhen, China Natl GeneBank, Shenzhen 518120, Peoples R China.;[Zhong, Yuhong] China Univ Geosci, Sch Environm Studies, Dept Biol Sci & Technol, Wuhan 430074, Hubei, Peoples R China.;[Hong, Yanyun] Hunan Agr Univ, Coll Plant Protect, Hunan Prov Key Lab Biol & Control Plant Pests, Changsha 410125, Hunan, Peoples R China.

通讯机构： [Zhou, CR; Liu, SL] B;[Zhou, Chengran] S;BGI Shenzhen, Shenzhen 518083, Peoples R China.;BGI Shenzhen, China Natl GeneBank, Shenzhen 518120, Peoples R China.;Sichuan Univ, Coll Life Sci, Key Lab Bioresource & Ecoenvironm, Minist Educ, Chengdu 610065, Sichuan, Peoples R China.

关键词： Population structure;Single nucleotide polymorphisms;Heterozygosity rate;Runs of homozygosity;Inbreeding;Hybridization

摘要： The wild Bactrian camel is one of the world’s rarest and most endangered animals and is facing hybridization threat due to human encroachment. Although captive breeding programs of the wild camel have been undertaken to save it from extinction, to succeed, we need to take into account many factors, e.g., maintain genetic diversity of captive populations similar to that found in wild populations. We applied restriction-site associated DNA sequencing (RAD-Seq) on a captive population in the most important wild camel conservation center in China—Gansu endangered animal conservation center—to investigate the genetic diversity, potential hybridization and inbreeding threats, and thus further guide population management. We sequenced a total of 44 Bactrian camels including 13 wild camels and 31 domestic camels and generated a genome-wide polymorphism dataset with 679,195 high-quality SNPs. This enabled us to detect wild camel individuals with recent genetic introgression from domestic camels. In addition, it revealed that the studied captive population suffers from inbreeding. We therefore suggest practical measures to improve the management of the wild camel and to protect its natural gene pool. © 2019, Springer Nature B.V.

语种： 英文

作者： Li, Qin;Li, Yongdi;Luo, Yu;Zhang, Yiyang;Chen, Yuan;...

期刊： Food Microbiology,2019年80:70-76 ISSN：0740-0020

通讯作者： Huang, Jianan;Liu, Zhonghua

作者机构： [Zhang, Yiyang; Liu, Zhonghua; Li, Qin; Wang, Kunbo; Huang, JN; Huang, Jianan; Li, Yongdi] Hunan Agr Univ, Key Lab Tea Sci, Minist Educ, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhonghua; Li, Qin; Huang, Jianan] Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhonghua; Li, Qin; Lin, Haiyan; Wang, Kunbo] Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Funct Ingredie, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhonghua; Li, Qin; Wang, Kunbo; Huang, Jianan] Hunan Agr Univ, Collaborat Innovat Ctr Utilizat Funct Ingredients, Changsha 410128, Hunan, Peoples R China.;[Luo, Yu] Zhejiang Univ, Inst Soil & Water Resources & Environm Sci, Hangzhou 3100058, Zhejiang, Peoples R China.

通讯机构： [Huang, JN; Liu, ZH] H;Hunan Agr Univ, Key Lab Tea Sci, Minist Educ, Changsha 410128, Hunan, Peoples R China.

关键词： Bacterial succession;Core functional bacteria;Food fermentation;Fu brick tea

摘要： To better understand the effects of bacteria on the characteristics of Fu brick tea, we investigated bacterial community structure as well as the predicted functions of identified bacteria and their correlations with chemical compounds during the manufacturing process. Overall, Klebsiella species dominated during the initial stage of processing, but were quickly replaced by Pseudomonas, Lactococcus, Stenotrophomonas, Enterococcus, and Bacillus species, which remained stable until the end of the manufacturing process. Network analysis identified 11 bacterial genera as keystone taxa, which contributed to the stabilization of the microbial community in the co-occurrence network. Bacterial taxa were grouped into eight modules, with the dominant genera mainly distributed amongst modules I and , which were involved in metabolism of carbon and flavor compounds in the Fu brick tea ecosystem. Using bidirectional orthogonal partial least squares analysis, 19 bacterial genera were identified as core functional genera linked to the metabolism of chemical compounds during the manufacturing process, while three genera, namely Klebsiella, Lactococcus, and Bacillus, also dominated the Fu brick tea fermentation process. These findings provide new insights into Fu Brick tea bacterial community variation and increased our understanding of the core functional bacterial genera involved in the manufacture of Fu brick tea.

语种： 英文

作者： Zhang, Ya;Liu, Qing;Wang, Chong*;Liu, Shuangqing;Shi, Li;...

期刊： International Journal of Agriculture and Biology,2019年22(6):1438-1444 ISSN：1560-8530

通讯作者： Liao, Xiaolan;Wang, Chong

作者机构： [Zhang, Ya; Liu, Shuangqing; Liu, Qing; Shi, Li; Liao, Xiaolan] Hunan Agr Univ, Coll Plant Protect, Dept Plant Protect, Changsha, Hunan, Peoples R China.;[Wang, Chong] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China.;[Liao, Xiaolan] Hunan Prov Key Lab Biol & Control Plant Dis & Pla, Changsha, Hunan, Peoples R China.

通讯机构： [Liao, Xiaolan; Wang, Chong] H;Hunan Agr Univ, Coll Plant Protect, Dept Plant Protect, Changsha, Hunan, Peoples R China.;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China.;Hunan Prov Key Lab Biol & Control Plant Dis & Pla, Changsha, Hunan, Peoples R China.

关键词： Botrytis cinerea;Cross-resistance;Mancozeb;New fungicides;Sensitivity

摘要： This study aimed to clarify the sensitivity and cross-resistance of Botrytis cinerea on strawberries to fluazinam (FAN), boscalid (BCI) and phenazine-1-carboxylic acid (PCA) to guide the rational use of these drugs. By measuring the mycelial growth rate, the sensitivity of 126 strains of B. cinerea on strawberries to FAN, BCI and PCA in 9 regions of Hunan Province was determined. The results showed that highest half maximal effective concentration (EC50) values for FAN, BCI and PCA against B. cinerea on strawberries were 0.9840 mu g/mL, 9.0564 mu g/mL and 11.2239 mu g/mL, respectively; the minimum values were 0.0135 mu g/mL, 0.1347 mu g/mL and 1.3540 mu g/mL, respectively, while the mean values were 0.1349 +/- 0.1710 mu g/mL, 2.1238 +/- 1.9095 mu g/mL and 5.2601 +/- 2.2908 mu g/mL, respectively. The highest values were 70.22-, 73.15- and 8.29-times higher, respectively, than the minimum values. The sensitivity frequencies of the three fungicides against B. cinerea on strawberries were not normally distributed, suggesting that sub-populations with reduced sensitivity were present in the B. cinerea population. Cross resistance to FAN, BCI and PCA existed. Field experiment results showed that the best control of B. cinerea occurred with FAN at 84.87%, which was 1.28-times that of the control, followed by BCI. PCA had the lowest control effect, which was lower than that of the control drug. This study provides a basis for decision-making in the future development of new policies for the prevention and control of B. cinerea. (C) 2019 Friends Science Publishers

语种： 英文

作者： Wang, Ping;Wang, Songbo;Chen, Yong;Xu, Xiaomin;Guang, Xuanmin;...

期刊： Computational Biology and Chemistry,2019年80:341-350 ISSN：1476-9271

通讯作者： Zhang, Youhua

作者机构： [Zhang, Youhua; Wang, Ping] Anhui Agr Univ, Sch Informat & Comp, Hefei 230036, Anhui, Peoples R China.;[Wang, Songbo] Hunan Agr Univ, Coll Plant Protect, Changsha 410000, Hunan, Peoples R China.;[Chen, Yong] State Tobacco Monopoly Adm, Sci & Technol Dept, Beijing 100045, Peoples R China.;[Xu, Xiaomin] UCSD, Dept Math, 9500 Gilman Dr, La Jolla, CA 92093 USA.;[Guang, Xuanmin] Zhejiang Univ, Coll Life Sci, Key Lab Conservat Biol Endangered Wildlife, Minist Educ, Hangzhou 310058, Zhejiang, Peoples R China.

通讯机构： [Zhang, Youhua] A;Anhui Agr Univ, Sch Informat & Comp, Hefei 230036, Anhui, Peoples R China.

关键词： Expression analysis;Gene family;MADS-box;Phylogenetic analysis;Watermelon

摘要： MADS-box genes comprise a family of transcription factors that function in the growth and development of plants. To obtain insights into their evolution in watermelon (Citrullus lanatus), we carried out a genome-wide analysis and identified 39 MADS-box genes. These genes were classified into MIKCc (25), MIKC*(3), Mα (5), Mβ (3), and Mγ (3)clades according to their phylogenetic relationship with Arabidopsis thaliana and Cucumis sativus; moreover, these 25 genes in the MIKC clade could be classified into 13 subfamilies, and the Flowering Locus C (FLC)subfamily is absent in watermelon. Analysis of the conserved gene motifs showed similar motifs among clades. Continuing chromosomal localizations analysis indicated that MADS-box genes were distributed across 11 chromosomes in watermelon, and these genes were conditioned to be differentially expressed during plant growth and development. This research provides information that will aid further investigations into the evolution of the MADS-box gene family in plants. © 2019 Elsevier Ltd

语种： 英文

作者： Shi, Li*;Shi, Yao;Zhang, Ya;Liao, Xiaolan*

期刊： Scientific Reports,2019年9(1):14997 ISSN：2045-2322

通讯作者： Shi, Li;Liao, Xiaolan

作者机构： [Shi, Li; Liao, XL; Zhang, Ya; Liao, Xiaolan; Shi, Yao] Hunan Agr Univ, Coll Plant Protect, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha 410128, Hunan, Peoples R China.;[Shi, Li; Liao, XL; Zhang, Ya; Liao, Xiaolan; Shi, Yao] Hunan Prov Engn & Technol Res Ctr Biopesticide &, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Shi, L; Liao, XL] H;Hunan Agr Univ, Coll Plant Protect, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha 410128, Hunan, Peoples R China.;Hunan Prov Engn & Technol Res Ctr Biopesticide &, Changsha 410128, Hunan, Peoples R China.

摘要： The tobacco cutworm, Spodoptera litura, is an important pest of crop and vegetable plants worldwide, and its resistance to insecticides have quickly developed. However, the resistance mechanisms of this pest are still unclear. In this study, the change in mRNA and miRNA profiles in the susceptible, indoxacarb-resistant and field indoxacarb-resistant strains of S. litura were characterized. Nine hundred and ten co-up-regulated and 737 co-down-regulated genes were identified in the resistant strains. Further analysis showed that 126 co-differentially expressed genes (co-DEGs) (cytochrome P450, carboxy/cholinesterase, glutathione S-transferase, ATP-binding cassette transporter, UDP-glucuronosyl transferase, aminopeptidase N, sialin, serine protease and cuticle protein) may play important roles in indoxacarb resistance in S. litura. In addition, a total of 91 known and 52 novel miRNAs were identified, and 10 miRNAs were co-differentially expressed in the resistant strains of S. litura. Furthermore, 10 co-differentially expressed miRNAs (co-DEmiRNAs) had predicted co-DEGs according to the expected miRNA-mRNA negative regulation pattern and 37 indoxacarb resistance-related co-DEGs were predicted to be the target genes. These results not only broadened our understanding of molecular mechanisms of insecticide resistance by revealing complicated profiles, but also provide important clues for further study on the mechanisms of miRNAs involved in indoxacarb resistance in S. litura.

语种： 英文

作者： He, Chao;Liang, Jinjin;Liu, Shaonan;Wang, Shaoli;Wu, Qingjun;...

期刊： Pesticide Biochemistry and Physiology,2019年153:136-143 ISSN：0048-3575

通讯作者： Xie, Wen;Zhang, Youjun

作者机构： [Liu, Shaonan; Xie, Wen; Zhang, Youjun; Wang, Shaoli; He, Chao; Wu, Qingjun; Xie, W; Zhang, YJ] Chinese Acad Agr Sci, Inst Vegetables & Flowers, Dept Plant Protect, Beijing 100081, Peoples R China.;[Liang, Jinjin] Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Xie, W; Zhang, YJ] C;Chinese Acad Agr Sci, Inst Vegetables & Flowers, Dept Plant Protect, Beijing 100081, Peoples R China.

关键词： ABC transporter;imidacloprid;insect protein;insecticide;neonicotinoid;nitro derivative;abdomen;animal;drug effect;female;gene expression regulation;genetics;head;Hemiptera;male;physiology;thorax;Abdomen;Animals;ATP-Binding Cassette Transporters;Female;Gene Expression Regulation;Head;Hemiptera;Insect Proteins;Insecticides;Male;Neonicotinoids;Nitro Compounds;Thorax

摘要： Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), a globally invasive species complex that causes serious damage to field crops, has developed resistance to imidacloprid and many other pesticides. Insect detoxify to pesticides may partially depend on ABC transporters, which contribute to the detoxification of xenobiotics. To determine whether genes in the ABCG subfamily are involved in imidacloprid detoxification in B. tabaci Q, we cloned four ABCG subfamily genes based on the published MED/Q genome and on our previous study of the transcriptional response of ABC transporters in B. tabaci Q adults to imidacloprid. As indicated by the quantification of mRNA levels after a 6-h exposure, the expression level of ABCG3 was 3.3-fold higher in B. tabaci Q adults exposed to 100 μg/mL imidacloprid rather than to the buffer control. The expression level of ABCG3 was higher in females than in males but did not significantly differ among eggs or nymphal stages and did not significantly differ among head, thorax, and abdomen tissues of adults. Knockdown of ABCG3 via RNA interference significantly increased the mortality of imidacloprid-treated laboratory and field-collected adults of B. tabaci Q. These results indicate that the ABCG3 gene may be involved in imidacloprid detoxification by B. tabaci Q. © 2018 Elsevier Inc.

语种： 英文

作者： Liu, Ao;Huang, Xiufang;Gong, Lijun;Guo, Zhaojiang;Zhang, Youjun;...

期刊： Pesticide Biochemistry and Physiology,2019年160:79-86 ISSN：0048-3575

通讯作者： Yang, Zhongxia

作者机构： [Liu, Ao; Huang, Xiufang; Yang, Zhongxia] Hunan Agr Univ, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Coll Plant Protect, Changsha, Hunan, Peoples R China.;[Gong, Lijun; Zhang, Youjun; Guo, Zhaojiang] Chinese Acad Agr Sci, Inst Vegetables & Flowers, Dept Plant Protect, Beijing, Peoples R China.;[Yang, Zhongxia] Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Yang, Zhongxia] H;Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.

关键词： bacterial protein;carrier protein;cytokine;endotoxin;hemolysin;insecticidal crystal protein, Bacillus Thuringiensis;monophenol monooxygenase;peptidoglycan recognition protein;amino acid sequence;animal;Bacillus thuringiensis;chemistry;gene expression;genetics;Lepidoptera;metabolism;molecular cloning;sequence homology;Amino Acid Sequence;Animals;Bacillus thuringiensis;Bacterial Proteins;Carrier Proteins;Cloning, Molecular;Cytokines;Endotoxins;Gene Expression;Hemolysin Proteins;Lepidoptera;Monophenol Monooxygenase;Sequence Homology, Amino Acid

摘要： Peptidoglycan recognition proteins (PGRPs) are important recognition receptors which play a critical role in signal identification and transmission in Toll or immune deficiency (IMD) pathways, particularly when pathogens evade and circumvent reactive oxygen species. Antimicrobial peptides (AMPs) synthesis can be activated by these signals to further eliminate pathogens. In this study, we cloned and characterized three different PGRP genes in Plutella xylostella strains, DBM1Ac-S, DBM1Ac-R and a field strain (DBM[sbnd]F). The results showed that PGRP1 belongs to the PGRP-SA family, PGRP2 to PGRP-LB, and PGRP3 to PGRP-LF. Moreover, PGRP1 expressed the highest transcript level, followed by PGRP3 and PGRP2, in two tissues including the gut and the larval carcass tissues of the DBM1Ac-S strain. Furthermore, altered expression levels of PGRP1-3 genes were detected in both gut and carcass tissues. Moreover, the DBM1Ac-R strain had the highest phenol oxidase (PO) activity among these three strains. The characterization of PGRP gene expression and PO activity in DBM1Ac-S, DBM1Ac-R and DBM-F provides insights into their important physiological roles in the immune system of P. xylostella exposed to Bt Cry1Ac toxin. © 2019 Elsevier Inc.

语种： 英文

作者： Sun, Miaomiao;Liu, Hao;Huang, Junbin;Peng, Jinbo;Fei, Fuhua;...

期刊： International Journal of Molecular Sciences,2019年20(8):1937-null ISSN：1661-6596

通讯作者： Zheng, Lu

作者机构： [Liu, Hao; Sun, Miaomiao; Huang, Junbin; Zheng, Lu] Huazhong Agr Univ, Key Lab Plant Pathol Hubei Prov, Wuhan 430070, Hubei, Peoples R China.;[Peng, Jinbo; Fei, Fuhua] Yichang Acad Agr Sci, Yichang 443004, Peoples R China.;[Zhang, Ya] Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.;[Hsiang, Tom] Univ Guelph, Sch Environm Sci, Guelph, ON N1G 2W1, Canada.

通讯机构： [Zheng, Lu] H;Huazhong Agr Univ, Key Lab Plant Pathol Hubei Prov, Wuhan 430070, Hubei, Peoples R China.

关键词： genomic DNA;primer DNA;Amorphophallus konjac;Article;bacterial soft rot;bacterial strain;bacterium detection;bioinformatics;colony forming unit;disease severity;DNA isolation;gene expression;gene sequence;genomics;limit of detection;loop mediated isothermal amplification;morphotype;nonhuman;pathogenicity;Pectobacterium;Pectobacterium aroidearum;real time polymerase chain reaction;rhizosphere;screening;sensitivity analysis;sensitivity and specificity;soil microflora;virus detection;whole genome sequencing;Amorphophallus;bacterial gene;genetics;isolation and purification;microbiology;nucleic acid amplification;Pectobacterium;plant disease;procedures;Amorphophallus;Genes, Bacterial;Nucleic Acid Amplification Techniques;Pectobacterium;Plant Diseases;Rhizosphere;Sensitivity and Specificity;Soil Microbiology

摘要： Bacterial soft rot caused by Pectobacterium species is a serious disease in konjac (Amorphophallus konjac), a healthy source of starch particularly in East Asia. An effective diagnostic method is crucial to control the disease and reduce losses in konjac production. In this study, we evaluated a loop-mediated isothermal amplification (LAMP) assay with a specific primer set for the rapid and accurate detection of P. Aroidearum. A comparative genomics approach was used to identify the specific genes suitable for the design of LAMP primers. The candidate target genes were determined through a first-round comparison with a 50-genome nucleotide database, and subjected to a second-round screening with the GenBank NR database. As a result, nine specific genes of P. aroidearum were selected for LAMP primer design. After screening of the primers, the primer set 1675-1 was chosen for LAMP detection owing to its high specificity and sensitivity. The LAMP assay could detect the presence of P. aroidearum genomic DNA at a concentration as low as 50 fg and 1.2 × 104 CFU/g artificially infected soil within 40 min at 65 °C. Subsequently, this primer set was successfully used to specifically detect P. aroidearum in naturally infected and non-symptomatic plant samples or soil samples from the field. This study indicates that a comparative genomic approach may facilitate the development of highly specific primers for LAMP assays, and a LAMP diagnostic assay with the specific primer set 1675-1 should contribute to the rapid and accurate detection of soft-rot disease in konjac at an early stage. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.

语种： 英文

作者： Yang, Yuting;Su, Qi;Shi, Linlin;Chen, Gong;Zeng, Yang;...

期刊： JOURNAL OF ECONOMIC ENTOMOLOGY,2019年112(4):1638-1644 ISSN：0022-0493

通讯作者： Zhang, Youjun

作者机构： [Yang, Yuting; Su, Qi; Shi, Caihua] Yangtze Univ, Coll Agr, Inst Insect Sci, Jingzhou 434025, Hubei, Peoples R China;[Yang, Yuting; Zeng, Yang; Zhang, Youjun] Chinese Acad Agr Sci, Inst Vegetables & Flowers, Dept Plant Protect, Beijing 100081, Peoples R China;[Shi, Linlin] Northeast Agr Univ, Harbin, Heilongjiang, Peoples R China;[Chen, Gong] Hunan Agr Univ, Coll Plant Protect, Changsha 410000, Hunan, Peoples R China

通讯机构： [Zhang, Youjun] C;Chinese Acad Agr Sci, Inst Vegetables & Flowers, Dept Plant Protect, Beijing 100081, Peoples R China.

关键词： Bradysia odoriphaga;Chinese chives;methyl allyl disulfide;diallyl disulfide;electroantennography

摘要： Bradysia odoriphaga Yang et Zhang is a serious belowground pest of Chinese chives (Allium tuberosum). Our previous studies have indicated that B. odoriphaga females prefer to oviposit near the roots of Chinese chives rather than the roots of other plants, and that the performance (longevity and fecundity) of B. odoriphaga offspring was better on Chinese chives than on Lettuce (var. ramosa Hort.), Onion (Allium cepa) and Potato (Solanum tuberosum) but little is known about how the volatiles released by Chinese chives affect the host-finding and oviposition behaviors of B. odoriphaga. Here, we used gas chromatography-mass spectrometry and determined that Chinese chives releases the following volatiles: methyl allyl disulfide, β-myrcene, cis-ocimene, diallyl disulfide, nonane, n-dodecane, n-tetradecane, and n-hexadecane; quantities released were highest for methyl allyl disulfide and diallyl disulfide. In addition to eliciting strong responses in females in electroantennography assays, the latter two sulfur compounds and their mixtures attracted females in Y-tube olfactometer assays. The addition of methyl allyl disulfide, diallyl disulfide, or a mixture of the two compounds at a 1:5 ratio to chive plants increased oviposition when compared to control plants. These results indicate that methyl allyl disulfide and diallyl disulfide, either alone or in combination, influence the host-seeking behavior of B. odoriphaga. © 2019 The Author(s). Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved.

语种： 英文