期刊：
International Journal of Biological Macromolecules,2025年:141860 ISSN：0141-8130
通讯作者：
Bai, Lianyang;Liu, Kailin
作者机构：
[Liu, Kailin; Chen, Hong; Zheng, Qianqi; Tu, Dingdi; Shao, Xiaolan; Yan, Bei; Liu, Xiangying; Bai, Lianyang] College of Plant Protection, Hunan Agricultural University, Changsha 410128, PR China;[Shao, Xiaolan] Hunan Weed Science Key Laboratory, Hunan Academy of Agricultural Sciences, Changsha 410125, PR China;[Deng, Peng] Hunan Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, PR China;[Li, Hui] Department of Crop and Soil Sciences, North Carolina State University, Raleigh, NC 27695, United States;[Dai, Jinfeng] Hunan Provincial Institute of Product and Goods Quality Inspection, Changsha 410007, PR China
摘要：
Fungicide contamination is a critical environmental issue, and the effective removal of fungicide residues from aquatic environments has attracted significant attention from researchers. In this study, the synthesized hyperbranched polysiloxane (TA-HBPSi) was grafted onto TEMPO-oxidized cellulose nanofibers (TOCNF) to fabricate a novel aerogel material (TA-HBPSi@TOCNF) with the aim of enhancing the sorption efficiency of fungicide. The equilibrium maximum adsorption capacity of TA-HBPSi@TOCNF for imazalil (8.04 mg/g) was significantly higher compared to other fungicides, including prochloraz (6.43 mg/g), thiophanate-methyl (4.12 mg/g), carbendazim (0.16 mg/g), and thiabendazole (0.04 mg/g). In the presence of Cd 2+ , the equilibrium adsorption capacity for imazalil increased from 8.04 mg/g to 9.61 mg/g, while the adsorption capacity for Cd 2+ increased from 0.26 mg/g to 0.88 mg/g. Quantum chemical calculations were performed at the density functional theory (DFT) level. The energy gaps between the highest occupied molecular orbital and lowest unoccupied molecular orbital (ΔE( HOMO-LUMO )) for various adsorption mechanisms indicated that electrostatic interactions were the dominant driving force for adsorption, followed by π-π electron donor-acceptor (EDA) interactions and hydrophobic interactions. The developed aerogel demonstrated effective multi-component separation capabilities without inducing secondary environmental pollution, highlighting its potential as a promising candidate for practical water purification applications.
Fungicide contamination is a critical environmental issue, and the effective removal of fungicide residues from aquatic environments has attracted significant attention from researchers. In this study, the synthesized hyperbranched polysiloxane (TA-HBPSi) was grafted onto TEMPO-oxidized cellulose nanofibers (TOCNF) to fabricate a novel aerogel material (TA-HBPSi@TOCNF) with the aim of enhancing the sorption efficiency of fungicide. The equilibrium maximum adsorption capacity of TA-HBPSi@TOCNF for imazalil (8.04 mg/g) was significantly higher compared to other fungicides, including prochloraz (6.43 mg/g), thiophanate-methyl (4.12 mg/g), carbendazim (0.16 mg/g), and thiabendazole (0.04 mg/g). In the presence of Cd 2+ , the equilibrium adsorption capacity for imazalil increased from 8.04 mg/g to 9.61 mg/g, while the adsorption capacity for Cd 2+ increased from 0.26 mg/g to 0.88 mg/g. Quantum chemical calculations were performed at the density functional theory (DFT) level. The energy gaps between the highest occupied molecular orbital and lowest unoccupied molecular orbital (ΔE( HOMO-LUMO )) for various adsorption mechanisms indicated that electrostatic interactions were the dominant driving force for adsorption, followed by π-π electron donor-acceptor (EDA) interactions and hydrophobic interactions. The developed aerogel demonstrated effective multi-component separation capabilities without inducing secondary environmental pollution, highlighting its potential as a promising candidate for practical water purification applications.
通讯机构：
[Chen, H ] H;Hunan Agr Univ, Sch Adm & Law, Changsha 410128, Peoples R China.
关键词：
Mobile internet use;Middle-aged and elderly people;Self-rated health;Internet
摘要：
The rapid development of digital technology has radically changed people’s lives. Simultaneously, as the population is rapidly aging, academic research is focusing on the use of Internet technology to improve middle-aged and older people’s health, particularly owing to the popularity of mobile networks, which has further increased the population’s accessibility to the Internet. However, related studies have not yet reached a consensus. Herein, empirical analysis of the influence of mobile Internet use on the subjective health and chronic disease status of individuals in their Middle Ages and above was conducted utilizing ordered logit, propensity score matching (PSM), and ordered probit models with data from the 2020 China Health and Retirement Longitudinal Study. The study aimed to provide a theoretical basis and reference for exploring technological advances to empower the development of a healthy Chinese population and to advance the process of healthy aging. The health of middle-aged and older adults mobile Internet users was greatly improved, according to our findings. Further, the use of mobile Internet by these persons resulted in improvements to both their self-assessed health and the state of their chronic diseases. As per the findings of the heterogeneity analysis, the impact of mobile Internet use was shown to be more pronounced on the well-being of middle-aged persons aged 45–60 years compared to those aged ≥ 60 years. Further, the endogeneity test revealed that the PSM model could better eliminate bias in sample selection. The results suggest that the estimates are more robust after eliminating endogeneity, and that failure to disentangle sample selectivity bias would overestimate not only the facilitating effect of mobile Internet use on the self-assessed health impacts of middle-aged and older adults, but also the ameliorating effect of mobile Internet use on the chronic diseases of middle-aged and older adults. The results of the mechanistic analysis suggest that social engagement is an important mediating mechanism between mobile Internet use and the health of middle-aged and older adults. This implies that mobile Internet use increases opportunities for social participation among middle-aged and older adults, thereby improving their health.
摘要：
Cis -13, 16-docosadienoic acid (DDA) is an omega -6 polyunsaturated fatty acid with great potential for application in medicine and health. Using microbial cell factories for DDA production is considered a viable alternative to extracting DDA from plant seeds. In this study, using Yarrowia lipolytica Po1f ( Delta ku70) as a chassis, firstly, the adaptation of three elongases in Po1f ( Delta ku70) were explored. Secondly, the DDA biosynthetic pathway was redesigned, resulting in a DDA content of 0.046 % of total fatty acids (TFAs). Thirdly, through the " push-pull " strategy, the DDA content increased to 0.078 % of TFAs. By enhancing the supply of acetyl-CoA, the DDA production in the engineered strain YL-7 reached 0.391 % of the TFAs (3.19 mg/L). Through optimizing the fermentation conditions, the DDA titer of YL-7 reached 29.34 mg/L. This research achieves the sustainable biological production of DDA in Y. lipolytica .
摘要：
Herbicides are the primary pesticides that can easily pollute water bodies, attracting significant attention from researchers regarding the effective removal of herbicide residues from the water environment. In this study, a β-cyclodextrin-reduced graphene oxide (β-CD-BTCA-rGO) aerogel was prepared following the self-assembly hydrothermal method using 1,2,3,4-butane tetracarboxylic acid (BTCA) as crosslinkers. The self-assembly process involved the cross-linking of “soft” β-cyclodextrin (β-CD) with “hard” graphene oxide (GO) nanosheets, forming a stable β-CD-BTCA-rGO aerogel. The prepared β-CD-BTCA-rGO aerogel demonstrated excellent adsorption performance. The adsorption capacities were 62.3 and 46.8 mg/g for sulfentrazone and quinclorac, respectively, surpassing the traditional reduced graphene oxide (rGO) aerogel and citric acid cross-linked (β-CD-CA-rGO) aerogel. Correspondingly, its adsorption capacity for quinclorac was 1.8 times and 12 times that of rGO aerogel and β-CD-CA-rGO aerogel, respectively. Quantum chemical calculations at the density functional theory (DFT) level, utilizing the Fukui function to predict chemical reaction sites, indicated that BTCA, as crosslinkers, formed stable chemical bonds (ester bonds) with GO through hydroxyl and carboxyl surface functional groups, leading to the formation of stable porous aerogels. Furthermore, the highest occupied molecular orbital-lowest unoccupied molecular orbital (HOMO-LUMO) energy gap was quantitatively measured for various adsorption mechanisms, revealing that electrostatic adsorption was the main force of the adsorption on the aerogels. The β-CD-BTCA-rGO aerogels prepared in this study demonstrated economic feasibility as adsorbents and exhibited good reproducibility. Consequently, β-CD-BTCA-rGO aerogels hold promising application prospects in the remediation of pesticide pollution in water systems.
Herbicides are the primary pesticides that can easily pollute water bodies, attracting significant attention from researchers regarding the effective removal of herbicide residues from the water environment. In this study, a β-cyclodextrin-reduced graphene oxide (β-CD-BTCA-rGO) aerogel was prepared following the self-assembly hydrothermal method using 1,2,3,4-butane tetracarboxylic acid (BTCA) as crosslinkers. The self-assembly process involved the cross-linking of “soft” β-cyclodextrin (β-CD) with “hard” graphene oxide (GO) nanosheets, forming a stable β-CD-BTCA-rGO aerogel. The prepared β-CD-BTCA-rGO aerogel demonstrated excellent adsorption performance. The adsorption capacities were 62.3 and 46.8 mg/g for sulfentrazone and quinclorac, respectively, surpassing the traditional reduced graphene oxide (rGO) aerogel and citric acid cross-linked (β-CD-CA-rGO) aerogel. Correspondingly, its adsorption capacity for quinclorac was 1.8 times and 12 times that of rGO aerogel and β-CD-CA-rGO aerogel, respectively. Quantum chemical calculations at the density functional theory (DFT) level, utilizing the Fukui function to predict chemical reaction sites, indicated that BTCA, as crosslinkers, formed stable chemical bonds (ester bonds) with GO through hydroxyl and carboxyl surface functional groups, leading to the formation of stable porous aerogels. Furthermore, the highest occupied molecular orbital-lowest unoccupied molecular orbital (HOMO-LUMO) energy gap was quantitatively measured for various adsorption mechanisms, revealing that electrostatic adsorption was the main force of the adsorption on the aerogels. The β-CD-BTCA-rGO aerogels prepared in this study demonstrated economic feasibility as adsorbents and exhibited good reproducibility. Consequently, β-CD-BTCA-rGO aerogels hold promising application prospects in the remediation of pesticide pollution in water systems.
关键词：
fine mapping;hybrid seed shattering;Oryza glaberrima;rice
摘要：
Seed shattering is an adaptive feature of seed dispersal in wild rice, and it is also an important agronomic trait affecting yield. Reduced seed shattering was a significant progress during rice domestication. However, the evolutionary pathway and molecular mechanism of hybrid seed shattering remain largely unknown. In order to gain a deeper understanding of the regulation of hybrid seed shattering, HS4, a locus conferring hybrid seed shattering between Oryza sativa and Oryza glaberrima, was identified and fine mapped to a 13.5-kb genomic region containing two putative genes during the development of chromosomal segment substitution lines (CSSLs). Expression analysis indicated that the hybrid seed shattering was not related to the expression of HS4. Preliminary research on the molecular mechanism of HS4-mediated hybrid seed shattering indicated that HS4(HJX74) and HS4(HP61) may form a multimer in heterozygotes, achieving the original function of a trihelix transcription factor through protein interaction. The identification and characterization of HS4 in this study not only provides new insights into the molecular mechanisms underlying hybrid seed shattering, but also provides a potential target for genome editing to reduce the difficulty of hybridization between the two species, facilitating hybrid breeding and increasing yield in rice.
期刊：
FRONTIERS IN IMMUNOLOGY,2024年15:1453272 ISSN：1664-3224
作者机构：
[Huang, Pengcheng; Deng, Wuquan] Department of Endocrinology and Metabolism, School of Medicine, Chongqing University Central Hospital, Chongqing Emergency Medical Centre, Chongqing University, China;[Hou, De-Xing] Kagoshima University, Kagoshima, Japan;[Wang, Qiang] Wuhan University of Science and Technology, China;[Chen, Hong] Sichuan Agricultural University, China;[Ding, Zhaoyun] National University of Defense Technology, China
关键词：
bioactive compounds;Inflammation;mechanisms of action;lifestyle interventions;Gut microbes
通讯机构：
[Wang, Y ] H;Hunan Agr Univ, Sch Publ Adm & Law, Changsha, Hunan, Peoples R China.;Hunan Univ Chinese Med, Sch Humanities & Management, Changsha, Hunan, Peoples R China.
关键词：
hierarchical medical system;population health;Self-assessed health;chronic disease status;Primary Care
摘要：
Introduction: The implementation of a hierarchical medical system holds significant practical importance in advancing the Healthy China strategy and elevating the overall health status of the population of China. Methods: This article empirically examines the influence of the implementation of a hierarchical medical system on the health of the population using the latest 2020 China Family Panel Studies database. Furthermore, it investigates the variability of this impact across distinct health insurance participation statuses and literacy groups. Results: The findings of the study demonstrate that the implementation of the hierarchical medical system substantially enhances the health of the population, not only in terms of self-assessed health but also through a notable positive effect on alleviating chronic diseases. These results maintain their validity even after conducting robustness tests utilizing a replacement estimation model. Heterogeneity analysis reveals that the impact of the hierarchical medical system on the population’s health status exhibits significant variation concerning health insurance participation and literacy. Specifically, regarding health insurance participation, the hierarchical medical system effectively improves both self-assessed health and chronic disease status among the insured population. However, for those not enrolled in health insurance, the hierarchical medical system only demonstrates improvement in chronic disease status, with insignificant results observed in enhancing self-assessed health status. Moreover, propensity score matching (PSM) was also used to address endogeneity problems resulting from sample selectivity bias. The findings demonstrate that endogeneity issues can be suitably addressed by the PSM model. Additionally, they point out that an overestimation of the impact of the hierarchical medical system on the population’s self-assessed health state would result from failing to take sample selectivity bias into account. On the other hand, it will lead to the underestimation of the effect of the hierarchical medical system on the status of chronic diseases. Discussion: Moving forward, steadfast efforts should be directed toward further enhancing the implementation of the hierarchical medical system. This includes the comprehensive promotion and using the pivotal role of the hierarchical medical system in improving the health of the population.
摘要：
Squalene is an important triterpene with a wide range of applications. Given the growing market demand for squalene, the development of microbial cell factories capable of squalene production is considered a sustainable method. This study aimed to investigate the squalene production potential of Yarrowia lipolytica. First, HMG-CoA reductase from Saccharomyces cerevisiae and squalene synthase from Y. lipolytica was co-overexpressed in Y. lipolytica. Second, by enhancing the supply of NADPH in the squalene synthesis pathway, the production of squalene in Y. lipolytica was effectively increased. Furthermore, by constructing an isoprenol utilization pathway and overexpressing YlDGA1, the strain YLSQ9, capable of producing 868.1 mg/L squalene, was obtained. Finally, by optimizing the fermentation conditions, the highest squalene concentration of 1628.2 mg/L (81.0 mg/g DCW) in Y. lipolytica to date was achieved. This study demonstrated the potential for achieving high squalene production using Y. lipolytica.
作者机构：
[Yu, Huan; Chen, Hong; Li, Ni; Xiao, Hua-Yan; Chen, Gong; Huang, Guo-Hua] Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha, Hunan, China;Agriculture and Rural Bureau of Xinhuang Dong Autonomous County, Huaihua, Hunan, China;[Yang, Chang-Jin] Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha, Hunan, China<&wdkj&>Agriculture and Rural Bureau of Xinhuang Dong Autonomous County, Huaihua, Hunan, China
通讯机构：
[Gong Chen; Guo-Hua Huang] H;Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha, Hunan, China
通讯机构：
[Liu, ZQ ] ;South China Agr Univ, Coll Agr, Guangdong Prov Key Lab Plant Mol Breeding, Guangzhou 510642, Guangdong, Peoples R China.;South China Agr Univ, State Key Lab Conservat & Utilizat Subtrop Agrobio, Guangzhou 510642, Guangdong, Peoples R China.
摘要：
<jats:title>Abstract</jats:title><jats:p>Understanding the evolutionary forces in speciation is a central goal in evolutionary biology. Asian cultivated rice has two subspecies, <jats:italic>indica</jats:italic> and <jats:italic>japonica</jats:italic>, but the underlying mechanism of the partial reproductive isolation between them remains obscure. Here we show a presence-absence variation (PAV) at the <jats:italic>Se</jats:italic> locus functions as an <jats:italic>indica-japonica</jats:italic> reproductive barrier by causing hybrid sterility (HS) in <jats:italic>indica-japonica</jats:italic> crosses. The locus comprises two adjacent genes: <jats:italic>ORF3</jats:italic> encodes a sporophytic pollen killer, whereas <jats:italic>ORF4</jats:italic> protects pollen in a gametophytic manner. In F<jats:sub>1</jats:sub> of <jats:italic>indica-japonica</jats:italic> crosses, pollen with the <jats:italic>japonica</jats:italic> haplotype, which lacks the sequence containing the protective <jats:italic>ORF4</jats:italic>, is aborted due to the pollen-killing effect of <jats:italic>ORF3</jats:italic> from <jats:italic>indica</jats:italic>. Evolutionary analysis suggests <jats:italic>ORF3</jats:italic> is a gene associated with the Asian cultivated rice species complex, and the PAV has contributed to the reproductive isolation between the two subspecies of Asian cultivated rice. Our analyses provide perspectives on rice inter-subspecies post-zygotic isolation, and will promote efforts to overcome reproductive barriers in <jats:italic>indica-japonica</jats:italic> hybrid rice breeding.</jats:p>
