作者机构：
[Juan Huang] School of Mechanical and Electrical Engineering, Hunan Agriculture University, Changsha, 410012, China;[Lairong Yin; Ronghua Du; Long Huang] School of Automotive and Mechanical Engineering, Changsha University of Science and Technology, Changsha, 410114, China;Hunan Provincial Key Laboratory of Intelligent Manufacturing Technology for High-performance Mechanical Equipment, Changsha University of Science and Technology, Changsha, 410114, China;[Jinhang Wang] School of Automotive and Mechanical Engineering, Changsha University of Science and Technology, Changsha, 410114, China<&wdkj&>Hunan Provincial Key Laboratory of Intelligent Manufacturing Technology for High-performance Mechanical Equipment, Changsha University of Science and Technology, Changsha, 410114, China
通讯机构：
[Lairong Yin] S;School of Automotive and Mechanical Engineering, Changsha University of Science and Technology, Changsha, 410114, China
摘要：
A rope-climbing robot (RCR) can reciprocate on a rope. To address the problems of poor load capacity and adaptability of the existing RCR, this study designs a dual-rope crawler type RCR, which can be used as a new type of transportation equipment in hilly, mountainous, and plateau areas. The crawler rope-climbing mechanism is a combination of a chain drive and the rope-climbing foot. Innovatively applying the parabolic theory of overhead rope to kinematically analyze the rope-climbing robot system, the robot motion trajectory model and the tilt angle equation are established. To establish the safe working interval of the rope-climbing robot, the influence of machine load and rope span on robot tilt angle is compared. Furthermore, research on the dynamic characteristics of the rope-climbing robot is carried out, establishing a time-varying system model of the dynamic tension of the rope in the rope-climbing robot system and analyzing the effects of speed and load on the dynamic tension of the rope and system stability. Finally, the prototype test results show that the RCR operates stably and has good load capacity and barrier-crossing capability.
摘要：
Cytochrome P450s play critical roles in the metabolic resistance of insecticides in insects. Previous findings showed that enhanced P450 activity was an important mechanism mediating indoxacarb resistance, and multiple P450 genes were upregulated in indoxacarb resistant strains of Spodoptera litura. However, the functions of these P450 genes in insecticide resistance remain unknown. Here, the P450 inhibitor PBO effectively decreased the resistance of S. litura to indoxacarb. Ten upregulated P450 genes were characterized, all of which were overexpressed in response to indoxacarb induction. Knockdown of nine P450 genes decreased cell viability against indoxacarb, and further silencing of three genes (CYP339A1, CYP340G2, CYP321A19) in larvae enhanced the sensitivity to indoxacarb. Transgenic overexpression of these three genes increased resistance to indoxacarb in Drosophila melanogaster. Moreover, molecular modeling and docking predicted that these three P450 proteins could bind tightly to indoxacarb and N-decarbomethoxylated metabolite (DCJW). Interestingly, these three P450 genes may also mediate cross-resistance to chlorantraniliprole, λ-cyhalothrin and imidacloprid. Additionally, heterologous expression and metabolic assays confirmed that three recombinant P450s could effectively metabolize indoxacarb and DCJW. This study strongly demonstrates that multiple overexpressed mitochondrial and microsomal P450 genes were involved in insecticide resistance in S. litura.
作者机构：
[Yao Zhou; Weisheng Wu; Ying Sun; Yiyu Shen; Lianzhen Mao; Yunhua Dai; Bozhi Yang; Zhoubin Liu] Engineering Research Center of Education Ministry for Germplasm Innovation and Breeding New Varieties of Horticultural Crops, Key Laboratory of Vegetable Biology of Hunan Province, College of Horticulture, Hunan Agricultural University, Changsha, China
通讯机构：
[Bozhi Yang; Zhoubin Liu] E;Engineering Research Center of Education Ministry for Germplasm Innovation and Breeding New Varieties of Horticultural Crops, Key Laboratory of Vegetable Biology of Hunan Province, College of Horticulture, Hunan Agricultural University, Changsha, China<&wdkj&>Engineering Research Center of Education Ministry for Germplasm Innovation and Breeding New Varieties of Horticultural Crops, Key Laboratory of Vegetable Biology of Hunan Province, College of Horticulture, Hunan Agricultural University, Changsha, China
关键词：
Chili pepper;Continuous photoperiod;Anthocyanin biosynthesis;Blue light
摘要：
Different metabolic compounds give pepper leaves and fruits their diverse colors. Anthocyanin accumulation is the main cause of the purple color of pepper leaves. The light environment is a critical factor affecting anthocyanin biosynthesis. It is essential that we understand how to use light to regulate anthocyanin biosynthesis in plants. Pepper leaves were significantly blue–purple only in continuous blue light or white light (with a blue light component) irradiation treatments, and the anthocyanin content of pepper leaves increased significantly after continuous blue light irradiation. This green-to-purple phenotype change in pepper leaves was due to the expression of different genes. We found that the anthocyanin synthesis precursor-related genes PAL and 4CL, as well as the structural genes F3H, DFR, ANS, BZ1, and F3’5’H in the anthocyanin synthesis pathway, had high expression under continuous blue light irradiation. Similarly, the expression of transcription factors MYB1R1-like, MYB48, MYB4-like isoform X1, bHLH143-like, and bHLH92-like isoform X3, and circadian rhythm-related genes LHY and COP1, were significantly increased after continuous blue light irradiation. A correlation network analysis revealed that these transcription factors and circadian rhythm-related genes were positively correlated with structural genes in the anthocyanin synthesis pathway. Metabolomic analysis showed that delphinidin-3-O-glucoside and delphinidin-3-O-rutinoside were significantly higher under continuous blue light irradiation relative to other light treatments. We selected 12 genes involved in anthocyanin synthesis in pepper leaves for qRT-PCR analysis, and the accuracy of the RNA-seq results was confirmed. In this study, we found that blue light and 24-hour irradiation together induced the expression of key genes and the accumulation of metabolites in the anthocyanin synthesis pathway, thus promoting anthocyanin biosynthesis in pepper leaves. These results provide a basis for future study of the mechanisms of light quality and photoperiod in anthocyanin synthesis and metabolism, and our study may serve as a valuable reference for screening light ratios that regulate anthocyanin biosynthesis in plants.
摘要：
Temperature is an important environmental factor affecting plant color, and different temperatures have distinct effects on plant leaf color changes. In order to explore the effects of different temperatures on plant color, we measured the physiological characteristics, transcriptome, and metabolome of the pepper leaf yellowing mutant yl1 and its wild type 6421 under different temperature conditions. Results showed that high temperature treatment compared with the normal temperature treatment significantly increased the contents of chlorophyll a, chlorophyll b, and carotenoids in yl1. At the same time, a total of 28 carotenoid pathway metabolites were detected under three temperature conditions. Among them, beta-carotene, zeaxanthin, violaxanthin, neoxanthin, lutein, and antheraxanthin were higher in pepper leaves. The expression levels of ZEP, bHLH104, MYB1R1, and MYB1R1-like were significantly increased under high temperature compared with under normal temperature, while the expression level of crtZ-2 and VDE were significantly decreased. Co-expression network analysis showed that VDE was positively correlated with the bHLH71-like and negatively correlated with MYB1R1-like and bHLH104. These results led to significant differences in the amount of zeaxanthin and antheraxanthin accumulated at different temperatures, which may be the reason for the color change of pepper leaves. However, under the normal and low temperature treatment, it is the opposite, resulting in leaf yellowing. This study provides a reference for further research on the mechanism underlying the impact of temperature on carotenoid metabolism in pepper leaves.
关键词：
Tourmaline;Bismuth Tungstate;Tetracycline;Photocatalysis;Electric field
摘要：
Bismuth tungstate (Bi2WO6) has been renowned for its affordability, non-toxic nature, and outstanding performance, but rapid carrier recombination has always been its fatal drawback. However, the inherent surface electric field of tourmaline effectively reduces carrier recombination and promotes their rapid separation. Consequently, the successful preparation of tourmaline/Bi2WO6 (T/BWO) composite materials has been achieved. Characterization and experiments indicate that, under the influence of the tourmaline electric field, the photocatalytic performance of Bi2WO6 is significantly enhanced. T/BWO successfully removed 95 % of tetracycline (TC) within 60 min. The reaction rate constant for T/BWO composite materials (0.0467 min -1) is 8.6-fold that of tourmaline (0.0054 min -1) and 4.0-fold that of Bi2WO6 (0.0118 min -1). Additionally, light absorption capability and carrier separation efficiency have been markedly improved. Through simulations of various natural environmental factors, T/BWO exhibits commendable practicality. Considering quenching experiments and the detection of active species, it has been determined that & sdot;O2- and h+ contribute the most, shedding light on its photocatalytic mechanism. Furthermore, the degradation process of tetracycline and the toxicity of its byproducts have been investigated. This study has contributed further innovations and insights to the enhancement of photocatalyst performance and efficient environmental remediation.
摘要：
Arsenic is a ubiquitous environmental pollutant. Microbe-mediated arsenic biotransformations significantly influence arsenic mobility and toxicity. Arsenic transformations by soil and aquatic organisms have been well documented, while little is known regarding effects due to endophytic bacteria. An endophyte Pseudomonas putida ARS1 was isolated from rice grown in arsenic contaminated soil. P. putida ARS1 shows high tolerance to arsenite (As(III)) and arsenate (As(V)), and exhibits efficient As(V) reduction and As(III) efflux activities. When exposed to 0.6 mg/L As(V), As(V) in the medium was completely converted to As(III) by P. putida ARS1 within 4 hr. Genome sequencing showed that P. putida ARS1 has two chromosomal arsenic resistance gene clusters ( arsRCBH ) that contribute to efficient As(V) reduction and As(III) efflux, and result in high resistance to arsenicals. Wolffia globosa is a strong arsenic accumulator with high potential for arsenic phytoremediation, which takes up As(III) more efficiently than As(V). Co-culture of P. putida ARS1 and W. globosa enhanced arsenic accumulation in W. globosa by 69%, and resulted in 91% removal of arsenic (at initial concentration of 0.6 mg/L As(V)) from water within 3 days. This study provides a promising strategy for in situ arsenic phytoremediation through the cooperation of plant and endophytic bacterium. (c) 2023 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.
作者机构：
College of Horticulture, Hunan Agricultural University, Changsha, Hunan 410128, China;Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding, Ministry of Education, Changsha, Hunan 410128, China;Key Laboratory for Vegetable Biology of Hunan Province, Changsha, Hunan 410128, China;Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops, Ministry of Agriculture and Rural Affairs of China, Changsha, Hunan 410128, China;[Qiuyun Wu; Qi Wu; Yuxiao Tian; Chunyan Zhou; Shuxiang Mao; Junwei Wang; Ke Huang] College of Horticulture, Hunan Agricultural University, Changsha, Hunan 410128, China<&wdkj&>Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding, Ministry of Education, Changsha, Hunan 410128, China<&wdkj&>Key Laboratory for Vegetable Biology of Hunan Province, Changsha, Hunan 410128, China<&wdkj&>Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops, Ministry of Agriculture and Rural Affairs of China, Changsha, Hunan 410128, China
通讯机构：
[Junwei Wang; Ke Huang] C;College of Horticulture, Hunan Agricultural University, Changsha, Hunan 410128, China<&wdkj&>Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding, Ministry of Education, Changsha, Hunan 410128, China<&wdkj&>Key Laboratory for Vegetable Biology of Hunan Province, Changsha, Hunan 410128, China<&wdkj&>Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops, Ministry of Agriculture and Rural Affairs of China, Changsha, Hunan 410128, China
关键词：
Brassica oleracea var. italica;Myrosinase;BoMY;Sulforaphane;Selenite treatment
摘要：
Sulforaphane, a naturally specialized metabolite, plays significant roles in human disease prevention and plant defense. Myrosinase (MY) is a key gene responsible for the catalysis of sulforaphane formation, but the molecular mechanisms through which MY regulates sulforaphane biosynthesis in plants remains largely unknown. Here, we discovered that the change of sulforaphane content in broccoli sprouts caused by exogenous selenite treatments is positively related to BoMY expression. BoMY overexpression in the Arabidopsis thaliana tgg1 mutants could dramatically increase myrosinase activity and sulforaphane content in the rosette leaves of 35S::BoMY/tgg1 and rescue its phenotypes. Moreover, an obvious increase of myrosinase activity and sulforaphane content was displayed in transgenic BoMY-overexpressed broccoli lines. In addition, a 2 033 bp promoter fragment of BoMY was isolated. Yeast one-hybrid (Y1H) library screening experiment uncovered that one bHLH transcription factor, BoFAMA, could directly bind to BoMY promoter to activate its expression, which was further evidenced by Y1H assay and dual-luciferase reporter assay. BoFAMA is a selenite-responsive transcription factor that is highly expressed in broccoli leaves; its protein is solely localized to nucleus. Additionally, genetic evidence suggested that the knockdown of FAMA gene in Arabidopsis thaliana could significantly decrease sulforaphane yield by inhibiting the expression of myrosinase genes. Interestingly, exogenous selenite supply could partially restore the low level of sulforaphane content in transgenic Arabidopsis FAMA-silencing plants. Our findings uncover a novel function of FAMA-MY module in the regulation of selenite-mediated sulforaphane synthesis and provide a new insights into the molecular mechanism by which selenite regulates the accumulation of sulforaphane in plants.
摘要：
This letter proposes a novel relaxation linear regression with spectral–spatial constrained locality adaptive regularization (SSLA‐RLR) method for HSI classification. The SSLA‐RLR method not only integrates the locality adaptive graph with relaxation linear regression to adaptively exploit the local geometrical structure for relieving the side effects of noise corruptions, but also takes the spatial correlations between each data point and its neighbours into consideration via spectral–spatial constraint. Abstract Recently, relaxation linear regression has received increasing attention in image analysis. However, the current relaxation linear regression methods fail to consider the local geometrical structure and spatial information while they are applied for hyperspectral image (HSI) classification. To address the above problems, this letter proposes a novel relaxation linear regression with spectral–spatial constrained locality adaptive regularization (SSLA‐RLR) method for HSI classification. The SSLA‐RLR method not only integrates the locality adaptive graph with relaxation linear regression to adaptively exploit the local geometrical structure for relieving the side effects of noise corruptions, but also takes the spatial correlations between each data point and its neighbours into consideration via spectral–spatial constraint. Finally, extensive experiments are conducted on three benchmark HSI datasets to demonstrate the effectiveness of the proposed method.
作者机构：
[Zhou, Ding; Wang, Hui] Hunan Agr Univ, Coll Water Resources & Civil Engn, Changsha 410128, Peoples R China.;[Wang, Xiangxiang] Anhui Jianzhu Univ, Sch Environm & Energy Engn, Hefei 230071, Peoples R China.;[Wang, Fangfang; Ma, Donghao; Zhang, Jiabao] Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, State Expt Stn Agroecosyst Fengqiu, Nanjing 210018, Peoples R China.
通讯机构：
[Ma, DH ] C;Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, State Expt Stn Agroecosyst Fengqiu, Nanjing 210018, Peoples R China.
关键词：
AquaCrop;North China Plain;water-stress simulation
摘要：
To apply AquaCrop to the study of agricultural soil moisture in the North China Plain, a water-treatment experiment on summer maize was carried out at the Fengqiu experimental station of the Chinese Academy of Sciences from 2017 to 2018. A water treatment was used to achieve field water capacities of 20 (W1), 40 (W2), and 60 (W3) cm soil layers under irrigation, and a rain-fed treatment (W0) was added. The model parameters were calibrated using the measured data in 2017. Then, they were applied to 2018 to verify the ability of the model to simulate water stress. The results showed that the variation trends for crop yield, canopy coverage, total soil water content, and the curve in the growth cycle simulated via AquaCrop were consistent with the actual observations; the results of a discrete analysis showed that the values of b (regression coefficient), R2 (determination coefficient), and EF (efficiency) were close to 1, and the values of the RMSE (root mean square error) were close to 0, which proved that the model could simulate dynamic changes in summer maize yield, canopy coverage, and total soil water content well. AquaCrop had good applicability in the North China Plain and could be applied to the study of agricultural water consumption and water-use efficiency in this area. The simulated values obtained can serve as an easily obtainable source of long-term experimental data in areas with frequent non-extreme weather events.
作者机构：
[Gao, Peng] Syracuse Univ, Dept Geog & Environm, Syracuse, NY 13244 USA.;[Gao, Peng] Univ Lyon, EUR Lyon H2O, Site ENS,15 Parvis R Descartes, F-69362 Lyon, France.;[Piegay, Herve; Belletti, Barbara] Univ Lyon, CNRS, EVS, UMR 5600, Site ENS,15 Parvis R Descartes, F-69007 Lyon, France.;[You, Yuchi] Hunan Agr Univ, Coll Water Resources & Civil Engn, Changsha 410128, Peoples R China.;[Li, Zhiwei] Wuhan Univ, State Key Lab Water Resources & Hydropower Engn Sc, Wuhan 430072, Peoples R China.
通讯机构：
[Gao, P ] S;Syracuse Univ, Dept Geog & Environm, Syracuse, NY 13244 USA.;Univ Lyon, EUR Lyon H2O, Site ENS,15 Parvis R Descartes, F-69362 Lyon, France.
关键词：
water-detection index;surface-water area;total mean water width;braiding index;daily discharge
摘要：
The use of water detection (WD) indices to infer daily discharge (Qd) has a great potential to enrich needed hydrological data for understanding fluvial processes driving the morphological changes of braided rivers. However, no consensus has been reached on which one stands out for use in mid-sized braided rivers. In this study, we compared the physical characteristics of three most commonly used WD indices, the Normalized Difference Water Index (NDWI), Modified Normalized Difference Water Index (MNDWI), and Normalized Difference Moisture Index (NDMI), for two mid-sized braided reach segments in the Qinghai-Tibet Plateau, China, that have different morphological structures. Relying on the Google Earth Engine web interface, we calculated the total mean water width (WWt) based on the detected surface-water areas (As) and braiding index (BI), as well as the mean values (m) of these indices over about four decades at the braided corridor scale (cs) (mNDWIcs, mMNDWIcs, and mNDMIcs). We then examined different responses of these indices to water and non-water features and their best threshold values for characterizing channel structures. Our analyses demonstrated that (1) NDWI and MNDWI perform well for detecting braided channel structures with the threshold of zero; (2) WWt is generally better correlated to Qd in a linear style than WD indices do, particularly when calculated from MNDWI; and (3) among WD indices calculated at the braided corridor scale, mMNDWIcs shows a better relationship with Qd than mNDMIcs does. Finally, we provided mechanisms that may explain these differences in terms of photometric discrepancies in calculating WWt and WD indices and the impact of image resolution on their calculations.
摘要：
The color of the seed coat has great diversity and is regarded as a biomarker of metabolic variations. Here we isolated a soybean variant (BLK) from a population of recombinant inbred lines with a black seed coat, while its sibling plants have yellow seed coats (YL). The BLK and YL plants showed no obvious differences in vegetative growth and seed weight. However, the BLK seeds had higher anthocyanins and flavonoids level and showed tolerance to various abiotic stresses including herbicide, oxidation, salt, and alkalinity during germination. Integrated metabolomic and transcriptomic analyses revealed that the upregulation of biosynthetic genes probably contributed to the overaccumulation of flavonoids in BLK seeds. The transient expression of those biosynthetic genes in soybean root hairs increased the levels of total flavonoids or anthocyanins. Our study revealed the molecular basis of flavonoid accumulation in soybean seeds, leveraging genetic engineering for both nutritious and stress-tolerant soybean germplasm.
通讯机构：
[Li, Y ] M;Minist Agr & Rural Affairs, Dept Anim Sci & Vet Med, Key Lab Efficient Utilizat Non Grain Feed Resource, Tai An, Peoples R China.;Dept Biosyst, Div Anim & Human Hlth Engn, Nutr & Anim Microbiota Ecosyst Lab, KU Leuven, Heverlee, Belgium.
摘要：
This study sought to explore the effects and potential mechanisms of dietary supplementation with isoquinoline alkaloids (IA) from Macleaya cordata to alleviate lipopolysaccharide (LPS)-induced intestinal epithelium injury in broilers. A total of 486 1-day-old broilers were assigned at random to a control (CON) group, LPS group, and LPS+IA group in a 21-d study. The CON and LPS groups received a basal diet, while the LPS+IA group received a basal diet supplemented with 0.6 mg/kg IA. At 17, 19, and 21 days of age, the LPS and LPS+BP groups were injected intraperitoneally with LPS, and the CON group was intraperitoneally injected equivalent amount of saline solution. The results manifested that LPS injection caused intestinal inflammation and lipid peroxidation, disrupted intestinal barrier and function, and increased the abundance of harmful microorganisms. However, dietary IA supplementation alleviated LPS-induced adverse changes in intestinal morphology, apoptosis, mucosal barrier integrity, cecum microorganisms, and homeostasis disorder by decreasing inflammatory cytokines and enhancing antioxidant-related genes expressions; inhibited LPS-induced increases in TLR4 and NF-κB expressions and decreases in Nrf2 and GPX1 genes expressions. Our findings indicated that Macleaya cordata IA addition attenuated LPS-induced intestinal epithelium injury and disorder of intestinal homeostasis by enhancing the anti-inflammatory and antioxidant capacity of broiler chickens possibly via co-regulating TLR4/MyD88/NF-κB and Nrf2 signaling pathways.
作者机构：
Hunan Key Laboratory of Traditional Chinese Veterinary Medicine, Hunan Agricultural University , Changsha 410128, Hunan, China;College of Veterinary Medicine, Hunan Agricultural University , Changsha 410128, Hunan, China;[Zhou, Li; Song, Rong] Hunan Institute of Agricultural Environment and Ecology, Hunan Academy of Agricultural Sciences , Changsha 410125, Hunan, China;[Zhong, Xiaohong] College of Horticulture, Hunan Agricultural University , Changsha 410128, Hunan, China;National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resource and Initiative, Hunan Agricultural University , Changsha 410128, Hunan, China
通讯机构：
[Peng Huang] C;[Jianguo Zeng] H;Hunan Key Laboratory of Traditional Chinese Veterinary Medicine, Hunan Agricultural University , Changsha 410128, Hunan, China<&wdkj&>College of Veterinary Medicine, Hunan Agricultural University , Changsha 410128, Hunan, China<&wdkj&>National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resource and Initiative, Hunan Agricultural University , Changsha 410128, Hunan, China<&wdkj&>College of Animal Science and Technology, Hunan Agricultural University , Changsha 410128, Hunan, China
摘要：
The Golden apple snail, Pomacea canaliculata, is one of the world's 100 worst invasive alien species that is best known for its damage to wetland agriculture. We analyzed the genetic variation and population genetics of P. canaliculata populations in seven regions of China based on molecular markers of three mitochondrial (mt) genes. Abstract The Golden apple snail, Pomacea canaliculata, is one of the world's 100 worst invasive alien species that is best known for its damage to wetland agriculture. It also acts as an intermediate host of some zoonotic parasites such as Angiostrongylus cantonensis, posing threats to human public health and safety. Despite is being an important agricultural pest, the genetic information and population expansion history of this snail remains poorly understood in China. In this study, we analyzed the genetic variation and population genetics of P. canaliculata populations in seven regions of China based on molecular markers of three mitochondrial (mt) genes. A total of 15 haplotypes were recognized based on single mt cox1, nad1, and nad4, and eight haplotypes were identified using the concatenated genes. High haplotype diversity, moderate nucleotide diversity, low gene flow, and high rates of gene differentiation among the seven P. canaliculata populations were detected. Shanghai and Yunnan populations showed higher genetic flow and very low genetic differentiation. The results of Tajima's D, Fu's Fs, and mismatch distribution showed that P. canaliculata did not experience population expansion in China. Genetic distance based on haplotypes suggested that nad1 gene was more conserved than cox1 gene within P. canaliculata. The phylogenetic analyses showed there may be two geographical lineages in the Chinese mainland. The present study may provide a new genetic marker to analyze P. canaliculata, and results support more evidence for studying the genetic distribution of P. canaliculata in China and contribute to a deeper understanding of its population genetics and evolutionary biology.
作者机构：
[Liu, Gang; Liu, Naiyuan; Liu, Zhuangzhuang; Zhao, Jinfeng; Fang, Jun; Wang, Keyu] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Liu, Gang; Liu, Naiyuan; Liu, Zhuangzhuang; Zhao, Jinfeng; Fang, Jun; Wang, Keyu] Hunan Engn Lab Pollut Control & Waste Utilizat Swi, Changsha 410128, Peoples R China.;[Yao, Hao] Changsha IMADEK Intelligent Technol Co Ltd, Changsha, Peoples R China.;[Pan, Junting] Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, State Key Lab Efficient Utilizat Arid & Semiarid A, Beijing 100081, Peoples R China.
通讯机构：
[Fang, J ] H;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;Hunan Engn Lab Pollut Control & Waste Utilizat Swi, Changsha 410128, Peoples R China.
关键词：
Meta genomic analysis;Network analysis;Rice straw-mushroom compost;Sawdust-rice husk compost
摘要：
This study aimed to assess the impact of microbial agent and different compost material, on physicochemical parameters dynamic change, nitrogen-transfer gene/bacterial community interaction network during the pig manure composting. Incorporating a microbial agent into rice straw-mushroom compost reduced the NH(3) and total ammonia emissions by 25.52% and 14.41%, respectively. Notably, rice straw-mushroom with a microbial agent reduced the total ammonia emissions by 37.67%. NH(4)(+)-N and pH emerged as primary factors of phylum-level and genus-level microorganisms. Microbial agent increased the expression of narG, nirK, and nosZ genes. Rice straw-mushroom elevated the content of amoA, nirK, nirS, and nosZ genes. Alcanivorax, Luteimonas, Pusillimonas, Lactobacillus, Aequorivita, Clostridium, Moheibacter and Truepera were identified as eight core microbial genera during the nitrogen conversion process. This study provides a strategy for reducing ammonia emissions and analyzes the potential mechanisms underlying compost processes.
